Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,945,896 T→C V63A (GTA→GCA)  yifE → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,945,8960TC99.7% 2112.8 / ‑4.4 658V63A (GTA→GCA) yifEhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (342/314);  total (344/314)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 683 total aligned reads displayed.

AACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACA  >  NC_000913/3945806‑3945985
                                                                                          |                                                                                         
  cTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGc                                                                                       >  1:4818848/1‑93 (MQ=255)
   tGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGcc                                                                                      >  2:1884102/1‑93 (MQ=255)
   tGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGcc                                                                                      >  1:5148090/1‑93 (MQ=255)
   tGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGcc                                                                                      >  1:2909841/1‑93 (MQ=255)
     cTTGAGCGTCGTGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    <  1:2589569/93‑1 (MQ=255)
     cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCgg                                                                                    >  2:303133/1‑92 (MQ=255)
     cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    >  1:1117676/1‑93 (MQ=255)
     cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    <  1:9696756/93‑1 (MQ=255)
     cTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGt                                                                                    >  2:7238776/1‑93 (MQ=255)
      ttGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTg                                                                                   >  2:318943/1‑93 (MQ=255)
       tGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTgg                                                                                  >  2:2366807/1‑93 (MQ=255)
           cGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGaa                                                                              <  1:9096067/93‑1 (MQ=255)
           cGTCAGTGTTATTCCTTCAATGAGTTTGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGaa                                                                              <  1:7701564/93‑1 (MQ=255)
            gTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAc                                                                             <  1:2433600/93‑1 (MQ=255)
             tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACg                                                                            >  2:4912117/1‑93 (MQ=255)
             tCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACg                                                                            >  2:3785805/1‑93 (MQ=255)
              cATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGt                                                                           >  2:5210361/1‑93 (MQ=255)
              cATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGt                                                                           >  2:4725800/1‑93 (MQ=255)
               aTGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTg                                                                          >  2:2783969/1‑93 (MQ=255)
               aTGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTg                                                                          <  2:434924/93‑1 (MQ=255)
                  gTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGc                                                                       <  1:5742142/93‑1 (MQ=255)
                   ttATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGcc                                                                      >  2:6375116/1‑93 (MQ=255)
                     aTGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAg                                                                    >  1:468923/1‑93 (MQ=255)
                     aTGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAg                                                                    >  2:7767128/1‑93 (MQ=255)
                      tGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGt                                                                   <  2:6212334/93‑1 (MQ=255)
                        ccTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGa                                                                 <  2:7029097/93‑1 (MQ=255)
                        ccTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGa                                                                 <  1:7029361/93‑1 (MQ=255)
                          ttCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACa                                                               >  2:6899941/1‑93 (MQ=255)
                            cAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGa                                                             >  2:4395008/1‑93 (MQ=255)
                             aaTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGaa                                                            <  2:9663875/93‑1 (MQ=255)
                              aTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAg                                                           <  2:8417485/93‑1 (MQ=255)
                              aTGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGCAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAg                                                           >  1:3865823/1‑93 (MQ=255)
                                gAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCa                                                         <  1:8896828/93‑1 (MQ=255)
                                 aGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAg                                                        <  1:6281862/93‑1 (MQ=255)
                                    tGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCTTAGCAGCATGCCGTGGCGAACGTGAGCTAGTGACAGAAGCAGAAc                                                     >  1:8981407/1‑93 (MQ=255)
                                      gATCTTGGCAAGCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgc                                                   <  2:9008121/93‑1 (MQ=255)
                                      gATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgg                                                   >  1:6651766/1‑92 (MQ=255)
                                       aTCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgcg                                                  >  2:8771502/1‑93 (MQ=255)
                                       aTCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgcg                                                  <  1:9475114/93‑1 (MQ=255)
                                       aTCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAcgcg                                                  >  1:1204955/1‑93 (MQ=255)
                                        tCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgt                                                 <  1:2291841/93‑1 (MQ=255)
                                         cTTGTCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgtg                                                <  2:4696988/93‑1 (MQ=255)
                                         cTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgtg                                                <  2:1647441/93‑1 (MQ=255)
                                          ttGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCgtgt                                               <  2:3454497/93‑1 (MQ=255)
                                           tGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGtgtg                                              >  1:5894177/1‑93 (MQ=255)
                                           tGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGtgtg                                              <  1:8761585/93‑1 (MQ=255)
                                            ggCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTgg                                             <  2:7563757/93‑1 (MQ=255)
                                            ggCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTgg                                             <  1:3946133/93‑1 (MQ=255)
                                              cAACCGCGAGCCGGTTCCCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAACCAGTGACAGAAGCAGAACGCGTGTGGTc                                           <  2:2189261/93‑1 (MQ=255)
                                              cAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTc                                           <  1:6403365/93‑1 (MQ=255)
                                               aaaCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTcc                                          >  2:3903265/1‑93 (MQ=255)
                                                aaCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCa                                         >  1:6201446/1‑93 (MQ=255)
                                                aaCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCa                                         >  2:9221602/1‑93 (MQ=255)
                                                 aCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCaa                                        >  1:8208665/1‑93 (MQ=255)
                                                    cgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGta                                     <  2:6756638/93‑1 (MQ=255)
                                                    cgAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGta                                     <  1:6849154/93‑1 (MQ=255)
                                                     gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtat                                    >  2:6126665/1‑93 (MQ=255)
                                                     gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtat                                    >  1:10034822/1‑93 (MQ=255)
                                                     gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtat                                    >  2:4077792/1‑93 (MQ=255)
                                                     gAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtat                                    <  1:6531675/93‑1 (MQ=255)
                                                      aGCCGGTTACCGAGGAAGAGAACCTCGTCATAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   <  2:1014649/93‑1 (MQ=255)
                                                      aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   <  1:7356990/93‑1 (MQ=255)
                                                      aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   <  1:4456865/93‑1 (MQ=255)
                                                      aGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGtata                                   >  2:3304282/1‑93 (MQ=255)
                                                       gCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTatat                                  <  1:1572743/93‑1 (MQ=255)
                                                       gCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTatat                                  >  2:5006722/1‑93 (MQ=255)
                                                        ccGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATg                                 <  1:7936401/93‑1 (MQ=255)
                                                         cGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGa                                <  2:9815087/93‑1 (MQ=255)
                                                         cGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGa                                <  1:9937852/93‑1 (MQ=255)
                                                          ggTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAc                               <  2:1743236/93‑1 (MQ=255)
                                                          ggTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAc                               >  2:6607042/1‑93 (MQ=255)
                                                           gTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAcg                              >  1:4996693/1‑93 (MQ=255)
                                                           gTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAcg                              >  2:5861735/1‑93 (MQ=255)
                                                            ttACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAcgc                             <  1:4229569/93‑1 (MQ=255)
                                                             tACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAcgcg                            >  2:1352352/1‑93 (MQ=255)
                                                              aCCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGt                           <  2:8173988/93‑1 (MQ=255)
                                                                 gAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAtt                        <  1:8596042/93‑1 (MQ=255)
                                                                  aGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGTAGAACGCGTGCGGTTCAAGTATATGACGCGTATTa                       >  1:3498642/1‑93 (MQ=255)
                                                                  aGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTa                       <  1:3166307/93‑1 (MQ=255)
                                                                   ggAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTaa                      <  2:1460923/93‑1 (MQ=255)
                                                                    gAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAg                     <  1:1662076/93‑1 (MQ=255)
                                                                    gAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAg                     <  1:7809062/93‑1 (MQ=255)
                                                                     aaGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGc                    <  1:3094155/93‑1 (MQ=255)
                                                                      agagAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCg                   >  1:7548743/1‑93 (MQ=255)
                                                                      agagAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCg                   <  2:5490604/93‑1 (MQ=255)
                                                                       gagaAGCTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGt                  <  1:6084477/93‑1 (MQ=255)
                                                                       gagaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGt                  >  1:1337962/1‑93 (MQ=255)
                                                                        agaAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTCAGCGTc                 >  2:7937467/1‑93 (MQ=255)
                                                                          aaaCTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCa               >  1:2025210/1‑93 (MQ=255)
                                                                           aaCTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCaa              <  1:5385189/93‑1 (MQ=255)
                                                                            aCTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCaaa             <  1:2164735/93‑1 (MQ=255)
                                                                                ttCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGt         >  2:5586731/1‑93 (MQ=255)
                                                                                   gTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtttt      <  2:2498415/93‑1 (MQ=255)
                                                                                   gTAGCAGCATGCCGTGGCGAACGTGAGCCAGCGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGtttt      <  1:2771368/93‑1 (MQ=255)
                                                                                    tAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTc     >  1:3700790/1‑93 (MQ=255)
                                                                                    gagcagcATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTc     <  1:1811918/92‑1 (MQ=255)
                                                                                     agcagcATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTca    <  2:4232320/93‑1 (MQ=255)
                                                                                     agcagcATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTca    >  1:93520/1‑93 (MQ=255)
                                                                                     agcagcATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTca    <  2:9609379/93‑1 (MQ=255)
                                                                                      gcagcaTGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGATTTcac   >  1:5447975/1‑93 (MQ=255)
                                                                                          |                                                                                         
AACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACA  >  NC_000913/3945806‑3945985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 406 total aligned reads displayed.
CACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCT  >  NC_000913/3945803‑3945989
                                                                                             |                                                                                             
CACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGC                                                                                               <  SRR4302540.5818329/94‑1 (MQ=60)
CACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGC                                                                                               >  SRR4302540.5597505/1‑94 (MQ=60)
CACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGC                                                                                               <  SRR4302540.267632/94‑1 (MQ=60)
 ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCA                                                                                              >  SRR4302540.2236782/1‑94 (MQ=60)
 ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCA                                                                                              >  SRR4302540.7960126/1‑94 (MQ=60)
 ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCA                                                                                              <  SRR4302540.8689516/94‑1 (MQ=60)
 ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCA                                                                                              >  SRR4302540.5973842/1‑94 (MQ=60)
 ACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTGGGATCTTGGCAAGCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCA                                                                                              <  SRR4302540.2163544/94‑1 (MQ=60)
  CAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAT                                                                                             <  SRR4302540.4777014/94‑1 (MQ=60)
  CAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAT                                                                                             <  SRR4302540.5330776/94‑1 (MQ=60)
  CAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAT                                                                                             <  SRR4302540.3797901/94‑1 (MQ=60)
  CAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAT                                                                                             <  SRR4302540.5863487/94‑1 (MQ=60)
  CAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTAGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCAT                                                                                             <  SRR4302540.9741564/94‑1 (MQ=60)
   AACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATG                                                                                            <  SRR4302540.6213468/94‑1 (MQ=60)
   AACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATG                                                                                            >  SRR4302540.9965746/1‑94 (MQ=60)
    ACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGC                                                                                           >  SRR4302540.4870431/1‑94 (MQ=60)
    ACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGC                                                                                           <  SRR4302540.9342643/94‑1 (MQ=60)
     CTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCC                                                                                          <  SRR4302540.4411329/94‑1 (MQ=60)
     CTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCC                                                                                          <  SRR4302540.1790036/94‑1 (MQ=60)
     CTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCC                                                                                          >  SRR4302540.1285002/1‑94 (MQ=60)
      TGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCG                                                                                         <  SRR4302540.3988567/94‑1 (MQ=60)
       GCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGT                                                                                        <  SRR4302540.1390998/94‑1 (MQ=60)
       GCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGT                                                                                        >  SRR4302540.1129266/1‑94 (MQ=60)
        CTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTG                                                                                       >  SRR4302540.4545670/1‑94 (MQ=60)
        CTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTG                                                                                       >  SRR4302540.6353790/1‑94 (MQ=60)
        CTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTG                                                                                       >  SRR4302540.2693599/1‑94 (MQ=60)
        CTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTG                                                                                       >  SRR4302540.6529804/1‑94 (MQ=60)
        CTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTG                                                                                       <  SRR4302540.9810772/94‑1 (MQ=60)
         TTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGG                                                                                      <  SRR4302540.8090521/94‑1 (MQ=60)
          TGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGC                                                                                     <  SRR4302540.1098763/94‑1 (MQ=60)
          TGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGC                                                                                     >  SRR4302540.6245229/1‑94 (MQ=60)
           GAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCG                                                                                    <  SRR4302540.9144864/94‑1 (MQ=60)
           GAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCG                                                                                    >  SRR4302540.5255025/1‑94 (MQ=60)
              CGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAC                                                                                 >  SRR4302540.3389918/1‑94 (MQ=60)
              CGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAC                                                                                 <  SRR4302540.9205354/94‑1 (MQ=60)
              CGTCAGTGTTATTCCTTCAATGAGTTTGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAC                                                                                 <  SRR4302540.7789586/94‑1 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                <  SRR4302540.2459819/94‑1 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.6574946/1‑94 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.9616203/1‑94 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.2391548/1‑94 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.4994244/1‑94 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.5671307/1‑94 (MQ=60)
               GTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACG                                                                                >  SRR4302540.7440560/1‑94 (MQ=60)
                gcgcgctttTGCCTTCAATGATTTGGTTGTTGGAAACCGGGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGT                                                                               <  SRR4302540.4988842/85‑1 (MQ=60)
                TCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGT                                                                               <  SRR4302540.3870137/94‑1 (MQ=60)
                 CATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTG                                                                              >  SRR4302540.8823399/1‑94 (MQ=60)
                  ATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAAGTCTTCGTAGCAGCATGCCGTGGCGCACGTGG                                                                             >  SRR4302540.9432769/1‑94 (MQ=60)
                  ATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGA                                                                             >  SRR4302540.9229100/1‑94 (MQ=60)
                   TGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAG                                                                            <  SRR4302540.7164969/94‑1 (MQ=60)
                   TGGTTATGCCTTCAATGAGTTGAATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAG                                                                            <  SRR4302540.3801853/94‑1 (MQ=60)
                    GGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGC                                                                           >  SRR4302540.2799773/1‑94 (MQ=60)
                    GGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGC                                                                           >  SRR4302540.8831288/1‑94 (MQ=60)
                     GTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCC                                                                          <  SRR4302540.5805440/94‑1 (MQ=60)
                      TTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCA                                                                         >  SRR4302540.7944506/1‑94 (MQ=60)
                       TATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAG                                                                        >  SRR4302540.473944/1‑94 (MQ=60)
                        ATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGT                                                                       <  SRR4302540.8321994/94‑1 (MQ=60)
                         TGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTG                                                                      <  SRR4302540.3185297/94‑1 (MQ=60)
                          GCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGA                                                                     >  SRR4302540.548444/1‑94 (MQ=60)
                           CCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGAC                                                                    >  SRR4302540.6917955/1‑94 (MQ=60)
                           CCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGAC                                                                    <  SRR4302540.7107466/94‑1 (MQ=60)
                            CTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACA                                                                   >  SRR4302540.8087889/1‑94 (MQ=60)
                            CTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACA                                                                   >  SRR4302540.7605759/1‑94 (MQ=60)
                            CTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACA                                                                   >  SRR4302540.5461484/1‑94 (MQ=60)
                            CTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACA                                                                   >  SRR4302540.5467538/1‑94 (MQ=60)
                             TTCAATGAGTTGGATCTTGGCAACCGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAG                                                                  <  SRR4302540.2655037/94‑1 (MQ=60)
                             TTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAG                                                                  <  SRR4302540.3458749/94‑1 (MQ=60)
                             TTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAG                                                                  <  SRR4302540.6986304/94‑1 (MQ=60)
                              TCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTCGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGA                                                                 >  SRR4302540.2107868/1‑94 (MQ=60)
                              TCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGA                                                                 >  SRR4302540.4956017/1‑94 (MQ=60)
                              TCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGA                                                                 <  SRR4302540.7651896/94‑1 (MQ=60)
                              TCAATGAGTTGCAGCGTGGAAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGA                                                                 <  SRR4302540.6607592/94‑1 (MQ=60)
                               TAATGATTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAA                                                                <  SRR4302540.3585149/94‑1 (MQ=60)
                               CAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAA                                                                <  SRR4302540.1985202/94‑1 (MQ=60)
                               CAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAA                                                                >  SRR4302540.9513834/1‑94 (MQ=60)
                                AATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGCAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGGCAGTGACAGAAG                                                               >  SRR4302540.4069368/1‑94 (MQ=60)
                                AATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAG                                                               >  SRR4302540.8923451/1‑94 (MQ=60)
                                AATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGCAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAG                                                               >  SRR4302540.3907844/1‑94 (MQ=60)
                                 ATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGC                                                              >  SRR4302540.903034/1‑94 (MQ=60)
                                   GAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAG                                                            <  SRR4302540.1204253/94‑1 (MQ=60)
                                   GAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAG                                                            <  SRR4302540.8999815/94‑1 (MQ=60)
                                     GTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAA                                                          <  SRR4302540.7021786/94‑1 (MQ=60)
                                     GTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAA                                                          <  SRR4302540.6257087/94‑1 (MQ=60)
                                      gtggctgtcgccaaagcggacgcggttacataggaaggaaacctcgtaGTAGCAGTAGGCCGGGGCGAACGTGAGCCAGTGACAGAAGCAGAAC                                                         <  SRR4302540.6018778/46‑1 (MQ=60)
                                      TTGGATCTTGGCAAACGCGAGCCGGTTACCGAGTAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAC                                                         <  SRR4302540.9794831/94‑1 (MQ=60)
                                      TTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCTTAGCAGCATGCCGTGGCGAACGTGAGCTAGTGACAGAAGCAGAAC                                                         >  SRR4302540.9088480/1‑94 (MQ=60)
                                      TTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAC                                                         <  SRR4302540.9493377/94‑1 (MQ=60)
                                      TTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAC                                                         >  SRR4302540.5129309/1‑94 (MQ=60)
                                      TTGGATCTTGACAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAAC                                                         <  SRR4302540.5909438/94‑1 (MQ=60)
                                       TGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACG                                                        <  SRR4302540.7074239/94‑1 (MQ=60)
                                        GGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGG                                                       >  SRR4302540.6725343/1‑94 (MQ=60)
                                        GGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGC                                                       >  SRR4302540.2094288/1‑94 (MQ=60)
                                         GATCTTGGCAAACGCGAGCCGGTTCCCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCG                                                      <  SRR4302540.2852470/94‑1 (MQ=60)
                                         GATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCG                                                      <  SRR4302540.2965096/94‑1 (MQ=60)
                                          ATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGT                                                     >  SRR4302540.7793697/1‑94 (MQ=60)
                                          ATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGT                                                     <  SRR4302540.8992041/94‑1 (MQ=60)
                                          ATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAAAGTGAGCCAGTGACAGAAGCAGAACGCGG                                                     >  SRR4302540.5075518/1‑94 (MQ=60)
                                           TCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTG                                                    <  SRR4302540.2316507/94‑1 (MQ=60)
                                            CTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGG                                                   >  SRR4302540.580522/1‑94 (MQ=60)
                                             TTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTG                                                  >  SRR4302540.3066027/1‑94 (MQ=60)
                                             TTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTG                                                  >  SRR4302540.5959275/1‑94 (MQ=60)
                                             TTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTG                                                  <  SRR4302540.6297446/94‑1 (MQ=60)
                                              TGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGG                                                 <  SRR4302540.786826/94‑1 (MQ=60)
                                              TGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGG                                                 <  SRR4302540.1188507/94‑1 (MQ=60)
                                              TGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGG                                                 >  SRR4302540.2064389/1‑94 (MQ=60)
                                              TGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGG                                                 <  SRR4302540.5755514/94‑1 (MQ=60)
                                                GCAAACGCGAGCAGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTC                                               >  SRR4302540.2490915/1‑94 (MQ=60)
                                                 CAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCC                                              <  SRR4302540.4615579/94‑1 (MQ=60)
                                                  AAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCA                                             >  SRR4302540.9181358/1‑94 (MQ=60)
                                                  AAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCA                                             <  SRR4302540.1903666/94‑1 (MQ=60)
                                                   AACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAA                                            >  SRR4302540.8882244/1‑94 (MQ=60)
                                                   AACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGAAGAACGCGTGTGGTCCAA                                            >  SRR4302540.2186108/1‑94 (MQ=60)
                                                    ACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAG                                           <  SRR4302540.748811/94‑1 (MQ=60)
                                                    ACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAG                                           <  SRR4302540.2940842/94‑1 (MQ=60)
                                                     CGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGT                                          <  SRR4302540.7577475/94‑1 (MQ=60)
                                                     CGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGA                                          >  SRR4302540.6221851/1‑94 (MQ=60)
                                                      GCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTA                                         >  SRR4302540.7805302/1‑94 (MQ=60)
                                                       CGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTAT                                        >  SRR4302540.176233/1‑94 (MQ=60)
                                                       CGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTAT                                        <  SRR4302540.5051921/94‑1 (MQ=60)
                                                       CGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTAT                                        <  SRR4302540.6925034/94‑1 (MQ=60)
                                                       CGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTAT                                        >  SRR4302540.9892176/1‑94 (MQ=60)
                                                        GAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATA                                       <  SRR4302540.7135962/94‑1 (MQ=60)
                                                        GAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATA                                       <  SRR4302540.3961013/94‑1 (MQ=60)
                                                         AGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATAT                                      >  SRR4302540.9575173/1‑94 (MQ=60)
                                                         AGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATAT                                      <  SRR4302540.5102571/94‑1 (MQ=60)
                                                         AGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATAT                                      <  SRR4302540.5985684/94‑1 (MQ=60)
                                                          GCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATG                                     <  SRR4302540.1589971/94‑1 (MQ=60)
                                                          GCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGGCCAAGTATATG                                     >  SRR4302540.2574357/1‑94 (MQ=60)
                                                           CCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGA                                    <  SRR4302540.8028898/94‑1 (MQ=60)
                                                           CCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGA                                    >  SRR4302540.2612845/1‑94 (MQ=60)
                                                            CGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAC                                   <  SRR4302540.10053830/94‑1 (MQ=60)
                                                            CGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAC                                   <  SRR4302540.9015573/94‑1 (MQ=60)
                                                            CGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGCGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGAC                                   >  SRR4302540.1514136/1‑94 (MQ=60)
                                                             GGTTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACG                                  >  SRR4302540.6392447/1‑94 (MQ=60)
                                                               TTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCG                                <  SRR4302540.6272107/94‑1 (MQ=60)
                                                               TTACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCG                                <  SRR4302540.4275611/94‑1 (MQ=60)
                                                                 ACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTA                              >  SRR4302540.3169700/1‑94 (MQ=60)
                                                                 ACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTA                              >  SRR4302540.4803226/1‑94 (MQ=60)
                                                                 ACCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTA                              <  SRR4302540.2028136/94‑1 (MQ=60)
                                                                  CCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAT                             >  SRR4302540.6492390/1‑94 (MQ=60)
                                                                  CCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAT                             <  SRR4302540.9046598/94‑1 (MQ=60)
                                                                  CCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAT                             <  SRR4302540.6435756/94‑1 (MQ=60)
                                                                  CCGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTAT                             <  SRR4302540.4111020/94‑1 (MQ=60)
                                                                   CGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATT                            >  SRR4302540.172649/1‑94 (MQ=60)
                                                                   CGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATT                            <  SRR4302540.3494757/94‑1 (MQ=60)
                                                                   CGAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATT                            <  SRR4302540.4432148/94‑1 (MQ=60)
                                                                    GAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTA                           >  SRR4302540.1630868/1‑94 (MQ=60)
                                                                    GAGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTA                           <  SRR4302540.8695964/94‑1 (MQ=60)
                                                                     AGGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAA                          <  SRR4302540.3200738/94‑1 (MQ=60)
                                                                      GGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAG                         >  SRR4302540.1341064/1‑94 (MQ=60)
                                                                      GGAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAG                         <  SRR4302540.4341057/94‑1 (MQ=60)
                                                                       GAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGC                        <  SRR4302540.1015148/94‑1 (MQ=60)
                                                                       GAAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGC                        >  SRR4302540.1160508/1‑94 (MQ=60)
                                                                        AAGAGAATATCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCG                       <  SRR4302540.1264865/94‑1 (MQ=60)
                                                                        AAGAGAAACTCTTCGTAGCAGCATGCTGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCG                       <  SRR4302540.6311939/94‑1 (MQ=60)
                                                                        AAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCG                       <  SRR4302540.3128079/94‑1 (MQ=60)
                                                                        AAGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCG                       >  SRR4302540.6097076/1‑94 (MQ=60)
                                                                        AAGAGAAACTCGTCGTAGCAGCATGCGGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGGGTGGTCCAAGTATATGACGCGTATTAAGCG                       <  SRR4302540.6840009/94‑1 (MQ=60)
                                                                         AGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGT                      >  SRR4302540.7679398/1‑94 (MQ=60)
                                                                         AGAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGT                      >  SRR4302540.1541435/1‑94 (MQ=60)
                                                                          GAGAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTC                     <  SRR4302540.2410075/94‑1 (MQ=60)
                                                                            GAAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCA                   <  SRR4302540.3601369/94‑1 (MQ=60)
                                                                             AAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTCTTAAGCGTCaca                  >  SRR4302540.6466669/1‑91 (MQ=60)
                                                                             AAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAA                  >  SRR4302540.7694867/1‑94 (MQ=60)
                                                                             AAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAA                  >  SRR4302540.1626748/1‑94 (MQ=60)
                                                                             AAACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAA                  >  SRR4302540.1224733/1‑94 (MQ=60)
                                                                              AACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAA                 <  SRR4302540.7176168/94‑1 (MQ=60)
                                                                              AACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAA                 <  SRR4302540.2294376/94‑1 (MQ=60)
                                                                              AACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAA                 <  SRR4302540.5444419/94‑1 (MQ=60)
                                                                              AACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAA                 <  SRR4302540.8277859/94‑1 (MQ=60)
                                                                               ACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAA                >  SRR4302540.8386443/1‑94 (MQ=60)
                                                                               ACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAA                >  SRR4302540.967471/1‑94 (MQ=60)
                                                                               ACTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAA                >  SRR4302540.2255513/1‑94 (MQ=60)
                                                                                CTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAAC               >  SRR4302540.16670/1‑94 (MQ=60)
                                                                                CTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAAC               <  SRR4302540.3050545/94‑1 (MQ=60)
                                                                                CTCTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAAC               >  SRR4302540.3629394/1‑94 (MQ=60)
                                                                                  CTTCGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGT             >  SRR4302540.884886/1‑94 (MQ=60)
                                                                                     CGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTT          <  SRR4302540.7946487/94‑1 (MQ=60)
                                                                                     CGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTT          >  SRR4302540.6335911/1‑94 (MQ=60)
                                                                                     CGTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTT          <  SRR4302540.6710884/94‑1 (MQ=60)
                                                                                      GTAGGAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTC         <  SRR4302540.2801318/94‑1 (MQ=60)
                                                                                      GTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTC         <  SRR4302540.6689351/94‑1 (MQ=60)
                                                                                      GTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTC         >  SRR4302540.3741189/1‑94 (MQ=60)
                                                                                      GTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTC         >  SRR4302540.458463/1‑94 (MQ=60)
                                                                                      GTAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGCCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTC         >  SRR4302540.1063798/1‑94 (MQ=60)
                                                                                       TAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCA        >  SRR4302540.94513/1‑94 (MQ=60)
                                                                                       TAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCA        >  SRR4302540.3908775/1‑94 (MQ=60)
                                                                                       TAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCA        <  SRR4302540.4762844/94‑1 (MQ=60)
                                                                                       GAGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCA        <  SRR4302540.1831691/94‑1 (MQ=60)
                                                                                        AGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCAC       >  SRR4302540.2350607/1‑94 (MQ=60)
                                                                                        AGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCAC       >  SRR4302540.4247624/1‑94 (MQ=60)
                                                                                        AGCAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGATTTCAC       >  SRR4302540.5507923/1‑94 (MQ=60)
                                                                                          CAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACAC     >  SRR4302540.354342/1‑94 (MQ=60)
                                                                                          CAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACAC     <  SRR4302540.8620860/94‑1 (MQ=60)
                                                                                          CAGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACAC     <  SRR4302540.5392223/94‑1 (MQ=60)
                                                                                           AGCATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACC    <  SRR4302540.2559130/94‑1 (MQ=60)
                                                                                             CATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAAGGTTTTCACACCCT  >  SRR4302540.3156477/1‑94 (MQ=60)
                                                                                             CATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCT  >  SRR4302540.2838483/1‑94 (MQ=60)
                                                                                             CATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCT  >  SRR4302540.4395125/1‑94 (MQ=60)
                                                                                             CATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCT  <  SRR4302540.6958104/94‑1 (MQ=60)
                                                                                             CATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGAGTGTGGTCAAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCAACCCCT  >  SRR4302540.2991932/1‑94 (MQ=60)
                                                                                             |                                                                                             
CACAACTGCTTGAGCGTCATGGTTATGCCTTCAATGAGTTGGATCTTGGCAAACGCGAGCCGGTTACCGAGGAAGAGAAACTCTTCGTAGCAGTATGCCGTGGCGAACGTGAGCCAGTGACAGAAGCAGAACGCGTGTGGTCCAAGTATATGACGCGTATTAAGCGTCCAAAACGTTTTCACACCCT  >  NC_000913/3945803‑3945989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑