Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,544,170 T→C I502I (ATT→ATC)  fimD → FimD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,544,1700TC99.8% 1925.5 / ‑5.8 589I502I (ATT→ATC) fimDFimD
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (315/273);  total (315/274)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 600 total aligned reads displayed.

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259
                                                                                          |                                                                                         
gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  2:825873/1‑93 (MQ=255)
gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  1:9121847/1‑93 (MQ=255)
 aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaa                                                                                        >  2:8393834/1‑93 (MQ=255)
   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      >  2:8873638/1‑93 (MQ=255)
   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      >  1:7633198/1‑93 (MQ=255)
   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      <  1:3009201/93‑1 (MQ=255)
    atTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAaca                                                                                     <  1:6713606/93‑1 (MQ=255)
     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacaa                                                                                    >  1:8347424/1‑92 (MQ=255)
      tCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAAcaca                                                                                   <  2:7377209/93‑1 (MQ=255)
       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  >  2:429687/1‑93 (MQ=255)
         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                <  2:9033764/93‑1 (MQ=255)
              tGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAg                                                                           <  2:6060867/93‑1 (MQ=255)
               gggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGt                                                                          >  1:5687414/1‑93 (MQ=255)
                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  1:9454511/93‑1 (MQ=255)
                   tACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAtt                                                                      <  1:6177466/93‑1 (MQ=255)
                    aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTc                                                                     >  1:1405484/1‑93 (MQ=255)
                     ccGTTATTCGACCAGCTGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCa                                                                    <  1:9000396/93‑1 (MQ=255)
                      cGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAg                                                                   >  1:2447269/1‑93 (MQ=255)
                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAgg                                                                  >  1:3742132/1‑93 (MQ=255)
                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAgg                                                                  >  2:1209578/1‑93 (MQ=255)
                            tCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAg                                                             <  1:7359279/93‑1 (MQ=255)
                             cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGc                                                            <  2:318923/93‑1 (MQ=255)
                             cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGc                                                            <  1:1295156/93‑1 (MQ=255)
                                 cAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGCATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGACGaa                                                        >  2:6757079/1‑93 (MQ=255)
                                 cAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaa                                                        >  2:7685714/1‑93 (MQ=255)
                                 cAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaa                                                        >  1:2653271/1‑93 (MQ=255)
                                 cAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaa                                                        <  2:7820909/93‑1 (MQ=255)
                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       <  2:6962545/93‑1 (MQ=255)
                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       >  1:4934781/1‑93 (MQ=255)
                                    cGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAtt                                                     <  2:5469536/93‑1 (MQ=255)
                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    <  2:2095396/93‑1 (MQ=255)
                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    <  1:9992582/93‑1 (MQ=255)
                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  2:7304286/1‑93 (MQ=255)
                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  1:7881206/1‑93 (MQ=255)
                                      gATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCa                                                   >  1:3482339/1‑93 (MQ=255)
                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  2:367675/1‑93 (MQ=255)
                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  2:2810205/93‑1 (MQ=255)
                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  1:2162710/1‑93 (MQ=255)
                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 <  2:6243253/93‑1 (MQ=255)
                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 <  2:4726752/93‑1 (MQ=255)
                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 >  1:3939646/1‑93 (MQ=255)
                                        tatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcc                                                 >  1:8559811/1‑93 (MQ=255)
                                         atTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAcca                                                >  2:9163630/1‑92 (MQ=255)
                                         atTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCg                                                >  2:5699887/1‑93 (MQ=255)
                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               >  1:5117072/1‑93 (MQ=255)
                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              >  2:5153483/1‑93 (MQ=255)
                                             tAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTa                                            <  2:170355/93‑1 (MQ=255)
                                             tAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTa                                            <  1:8004262/93‑1 (MQ=255)
                                              aaTTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAt                                           <  2:8512790/93‑1 (MQ=255)
                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        >  1:4536528/1‑93 (MQ=255)
                                                  tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACa                                       >  2:9404665/1‑93 (MQ=255)
                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      <  1:9790372/93‑1 (MQ=255)
                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     >  1:9640454/1‑93 (MQ=255)
                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  1:5962454/93‑1 (MQ=255)
                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:2540031/93‑1 (MQ=255)
                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  >  2:584959/1‑93 (MQ=255)
                                                        aTACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCg                                 <  2:4226795/93‑1 (MQ=255)
                                                          acaacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCt                               >  1:8204917/1‑93 (MQ=255)
                                                           caacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCtt                              <  1:6585687/93‑1 (MQ=255)
                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             <  1:1575876/93‑1 (MQ=255)
                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             <  2:8124561/93‑1 (MQ=255)
                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:1662898/1‑93 (MQ=255)
                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            <  2:4604623/93‑1 (MQ=255)
                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            <  1:4187797/93‑1 (MQ=255)
                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  1:7504530/1‑93 (MQ=255)
                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           <  2:2664985/93‑1 (MQ=255)
                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           <  2:4055126/93‑1 (MQ=255)
                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           <  2:4864733/93‑1 (MQ=255)
                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          <  1:6839450/93‑1 (MQ=255)
                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:3458042/1‑93 (MQ=255)
                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:6544129/1‑93 (MQ=255)
                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  1:307122/1‑93 (MQ=255)
                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:4486370/1‑93 (MQ=255)
                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       >  2:2482514/1‑93 (MQ=255)
                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       <  1:7198226/93‑1 (MQ=255)
                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      >  2:1473681/1‑93 (MQ=255)
                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      >  1:10020786/1‑93 (MQ=255)
                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      <  1:4699160/93‑1 (MQ=255)
                                                                     tCGAATGAATGGCTACAACCTCGAAACACAGGACGGAGTTATTCCGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAAAAAcg                    >  2:3565676/1‑93 (MQ=255)
                                                                     tCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                    >  1:1203251/1‑93 (MQ=255)
                                                                     tCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                    >  1:71006/1‑93 (MQ=255)
                                                                     tCGAATGAATGGCTACAAAATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                    <  2:9709369/93‑1 (MQ=255)
                                                                       gaatgaatGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgcg                  >  1:5410210/1‑93 (MQ=255)
                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:1892795/1‑93 (MQ=255)
                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  2:7904148/1‑93 (MQ=255)
                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  1:630979/1‑93 (MQ=255)
                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  2:8838761/93‑1 (MQ=255)
                                                                             atgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAt            >  2:2962562/1‑93 (MQ=255)
                                                                              tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           >  1:5584060/1‑93 (MQ=255)
                                                                              tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           >  1:4108429/1‑93 (MQ=255)
                                                                              tgGCGACAACATCAAAACACAGGACTGAGTTATTCAGGTTAAGACGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           <  1:2886357/93‑1 (MQ=255)
                                                                               ggCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTa          >  2:2220673/1‑93 (MQ=255)
                                                                               ggCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTa          <  1:9932952/93‑1 (MQ=255)
                                                                                 cTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACa        <  2:6609759/93‑1 (MQ=255)
                                                                                  tACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa       >  1:5299805/1‑93 (MQ=255)
                                                                                     aacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTc    >  2:7103943/1‑93 (MQ=255)
                                                                                     aacaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTc    >  1:9374814/1‑93 (MQ=255)
                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCa   >  1:1910318/1‑93 (MQ=255)
                                                                                      acaTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC‑ACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  <  1:6381362/93‑1 (MQ=255)
                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  <  1:8797928/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 325 total aligned reads displayed.
CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTT  >  NC_000913/4544077‑4544263
                                                                                             |                                                                                             
CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATC                                                                                               <  SRR4302540.251102/94‑1 (MQ=60)
CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATC                                                                                               <  SRR4302540.6770245/94‑1 (MQ=60)
CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATC                                                                                               <  SRR4302540.6145701/94‑1 (MQ=60)
 ACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCG                                                                                              <  SRR4302540.433804/94‑1 (MQ=60)
  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                             <  SRR4302540.10076980/94‑1 (MQ=60)
  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                             <  SRR4302540.7268179/94‑1 (MQ=60)
  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                             >  SRR4302540.7062378/1‑94 (MQ=60)
  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                             <  SRR4302540.6934006/94‑1 (MQ=60)
  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                             >  SRR4302540.9231495/1‑94 (MQ=60)
   GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAA                                                                                            >  SRR4302540.8473202/1‑94 (MQ=60)
     ATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAAC                                                                                          >  SRR4302540.29817/1‑94 (MQ=60)
     ATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAAC                                                                                          >  SRR4302540.7719071/1‑94 (MQ=60)
     ATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAAC                                                                                          >  SRR4302540.7867891/1‑94 (MQ=60)
      TATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACA                                                                                         <  SRR4302540.4750017/94‑1 (MQ=60)
      TATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACA                                                                                         <  SRR4302540.446735/94‑1 (MQ=60)
      TATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACA                                                                                         <  SRR4302540.3042313/94‑1 (MQ=60)
       ATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACAC                                                                                        <  SRR4302540.6787685/94‑1 (MQ=60)
       ATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACAA                                                                                        >  SRR4302540.8444303/1‑94 (MQ=60)
        TTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACA                                                                                       <  SRR4302540.3702100/94‑1 (MQ=60)
        TTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACA                                                                                       >  SRR4302540.2999138/1‑94 (MQ=60)
          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACATG                                                                                     >  SRR4302540.9591564/1‑94 (MQ=60)
          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                     >  SRR4302540.8325400/1‑94 (MQ=60)
          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                     <  SRR4302540.1494588/94‑1 (MQ=60)
          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                     >  SRR4302540.129204/1‑94 (MQ=60)
          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                     >  SRR4302540.7550617/1‑94 (MQ=60)
           AGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGA                                                                                    >  SRR4302540.3390033/1‑94 (MQ=60)
           AGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGA                                                                                    >  SRR4302540.1857042/1‑94 (MQ=60)
            GTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGAG                                                                                   >  SRR4302540.1331367/1‑94 (MQ=60)
            GTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGAC                                                                                   >  SRR4302540.8887405/1‑94 (MQ=60)
            GTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGAC                                                                                   <  SRR4302540.6393106/94‑1 (MQ=60)
             TTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACG                                                                                  <  SRR4302540.5756351/94‑1 (MQ=60)
              TAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGG                                                                                 <  SRR4302540.6081179/94‑1 (MQ=60)
              TAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGG                                                                                 >  SRR4302540.2908280/1‑94 (MQ=60)
               AGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGACACACAGGACGGA                                                                                <  SRR4302540.9994464/94‑1 (MQ=60)
               AGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGA                                                                                <  SRR4302540.4337255/94‑1 (MQ=60)
                 TGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGT                                                                              >  SRR4302540.5750018/1‑94 (MQ=60)
                  GGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACGTACAGTCGAATGAGTGGCTACAACATCGAAACACAGGACGGAGTT                                                                             <  SRR4302540.171310/94‑1 (MQ=60)
                  GGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTT                                                                             >  SRR4302540.8627132/1‑94 (MQ=60)
                  GGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTT                                                                             <  SRR4302540.5978730/94‑1 (MQ=60)
                   GGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTA                                                                            >  SRR4302540.10078582/1‑94 (MQ=60)
                    GTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAT                                                                           >  SRR4302540.1615871/1‑94 (MQ=60)
                    GTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAT                                                                           <  SRR4302540.6770934/94‑1 (MQ=60)
                    GTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAT                                                                           >  SRR4302540.1327588/1‑94 (MQ=60)
                    GTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAT                                                                           <  SRR4302540.6588443/94‑1 (MQ=60)
                     TTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATT                                                                          <  SRR4302540.242415/94‑1 (MQ=60)
                     TTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATT                                                                          >  SRR4302540.6876768/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.4646813/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         <  SRR4302540.4839243/94‑1 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         <  SRR4302540.1447752/94‑1 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.1420621/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.8153545/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.2115602/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.2230587/1‑94 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         <  SRR4302540.1075709/94‑1 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         <  SRR4302540.10100927/94‑1 (MQ=60)
                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                         >  SRR4302540.4646812/1‑94 (MQ=60)
                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                        >  SRR4302540.141470/1‑94 (MQ=60)
                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                        <  SRR4302540.6687712/94‑1 (MQ=60)
                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                        <  SRR4302540.77988/94‑1 (MQ=60)
                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                        <  SRR4302540.7762072/94‑1 (MQ=60)
                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                        >  SRR4302540.9125679/1‑94 (MQ=60)
                        CCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAG                                                                       >  SRR4302540.2473622/1‑94 (MQ=60)
                        CCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAG                                                                       >  SRR4302540.5220799/1‑94 (MQ=60)
                         CGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGG                                                                      >  SRR4302540.3299935/1‑94 (MQ=60)
                         CGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGG                                                                      >  SRR4302540.4664613/1‑94 (MQ=60)
                         CGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGG                                                                      <  SRR4302540.8098121/94‑1 (MQ=60)
                          GTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGT                                                                     <  SRR4302540.5422252/94‑1 (MQ=60)
                          GTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGT                                                                     <  SRR4302540.7615230/94‑1 (MQ=60)
                          GTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGT                                                                     >  SRR4302540.8895603/1‑94 (MQ=60)
                          GTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGT                                                                     >  SRR4302540.9553917/1‑94 (MQ=60)
                             ATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAA                                                                  <  SRR4302540.5068101/94‑1 (MQ=60)
                             ATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGGCGAATGAATGGCTAAAACATCGAAACAAAGGACGGcgtactccagattcg                                                                  >  SRR4302540.5375383/1‑79 (MQ=60)
                               TCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGC                                                                >  SRR4302540.6272384/1‑94 (MQ=60)
                               TCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGC                                                                <  SRR4302540.5211849/94‑1 (MQ=60)
                               TCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGC                                                                <  SRR4302540.7441347/94‑1 (MQ=60)
                               TCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGC                                                                <  SRR4302540.7254820/94‑1 (MQ=60)
                                CGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGTAGTTATTCAGGTTAAGCC                                                               <  SRR4302540.242698/94‑1 (MQ=60)
                                 GACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCG                                                              <  SRR4302540.3976286/94‑1 (MQ=60)
                                  ACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGA                                                             <  SRR4302540.8402342/94‑1 (MQ=60)
                                   CCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAA                                                            <  SRR4302540.4060329/94‑1 (MQ=60)
                                   CCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAA                                                            >  SRR4302540.2682057/1‑94 (MQ=60)
                                    CAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAA                                                           >  SRR4302540.6191906/1‑94 (MQ=60)
                                    CAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAA                                                           >  SRR4302540.4988006/1‑94 (MQ=60)
                                     AGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAT                                                          >  SRR4302540.3764526/1‑94 (MQ=60)
                                      GCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACACCATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATC                                                         >  SRR4302540.766729/1‑94 (MQ=60)
                                      GCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATT                                                         >  SRR4302540.8219267/1‑94 (MQ=60)
                                      GCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATT                                                         >  SRR4302540.766730/1‑94 (MQ=60)
                                       CGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTC                                                        <  SRR4302540.6408070/94‑1 (MQ=60)
                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCC                                                       >  SRR4302540.2038754/1‑94 (MQ=60)
                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCA                                                       >  SRR4302540.7973233/1‑94 (MQ=60)
                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCA                                                       >  SRR4302540.3520257/1‑94 (MQ=60)
                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCA                                                       <  SRR4302540.9813138/94‑1 (MQ=60)
                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCA                                                       >  SRR4302540.5456169/1‑94 (MQ=60)
                                         GATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAC                                                      >  SRR4302540.2186005/1‑94 (MQ=60)
                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGTCTACAACATCGAAACACAGGACGGAGGTATTCAGGTTAAGCCGAAATTCACC                                                     >  SRR4302540.2811091/1‑94 (MQ=60)
                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                     >  SRR4302540.1210909/1‑94 (MQ=60)
                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                     <  SRR4302540.7248918/94‑1 (MQ=60)
                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                     >  SRR4302540.3982209/1‑94 (MQ=60)
                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                     >  SRR4302540.4853650/1‑94 (MQ=60)
                                           TATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCG                                                    >  SRR4302540.3618888/1‑94 (MQ=60)
                                           TATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCG                                                    >  SRR4302540.5196690/1‑94 (MQ=60)
                                            ATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGA                                                   >  SRR4302540.94781/1‑94 (MQ=60)
                                            ATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCCAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGA                                                   >  SRR4302540.94782/1‑94 (MQ=60)
                                              TTTAATTTCGCTGATACAACATACAGTCGAATGAATGGTTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACT                                                 <  SRR4302540.4175008/94‑1 (MQ=60)
                                               TTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTA                                                >  SRR4302540.1291613/1‑94 (MQ=60)
                                               TTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTA                                                >  SRR4302540.1025180/1‑94 (MQ=60)
                                               TTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTA                                                <  SRR4302540.1845696/94‑1 (MQ=60)
                                                TAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAT                                               <  SRR4302540.2037130/94‑1 (MQ=60)
                                                TAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAT                                               <  SRR4302540.9869074/94‑1 (MQ=60)
                                                TAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAT                                               >  SRR4302540.5001225/1‑94 (MQ=60)
                                                 AATTTCGCTGATACAACATACAGTCGAATGAATGGCCACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATT                                              <  SRR4302540.6306357/94‑1 (MQ=60)
                                                  ATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTA                                             >  SRR4302540.7997743/1‑94 (MQ=60)
                                                    TTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                           <  SRR4302540.6124885/94‑1 (MQ=60)
                                                    TTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                           >  SRR4302540.3038249/1‑94 (MQ=60)
                                                    TTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                           <  SRR4302540.6349055/94‑1 (MQ=60)
                                                    TTCGCTGATACAACATACAGTCGAATCAATGGCTACAACATCTAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                           <  SRR4302540.5418745/94‑1 (MQ=60)
                                                     TCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAA                                          >  SRR4302540.5224801/1‑94 (MQ=60)
                                                      tcCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAC                                         <  SRR4302540.5677628/92‑1 (MQ=60)
                                                      CGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAC                                         >  SRR4302540.9754019/1‑94 (MQ=60)
                                                      CGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAC                                         <  SRR4302540.6017717/94‑1 (MQ=60)
                                                      CGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAC                                         <  SRR4302540.9905065/94‑1 (MQ=60)
                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                        <  SRR4302540.2633813/94‑1 (MQ=60)
                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                        >  SRR4302540.7339722/1‑94 (MQ=60)
                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                        <  SRR4302540.1306950/94‑1 (MQ=60)
                                                          GATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCG                                     <  SRR4302540.9115056/94‑1 (MQ=60)
                                                          GATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCG                                     >  SRR4302540.420332/1‑94 (MQ=60)
                                                           ATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGC                                    >  SRR4302540.8934441/1‑94 (MQ=60)
                                                           ATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGC                                    >  SRR4302540.7077898/1‑94 (MQ=60)
                                                           ATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGC                                    >  SRR4302540.5778862/1‑94 (MQ=60)
                                                            TACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCT                                   <  SRR4302540.9361734/94‑1 (MQ=60)
                                                            TACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCT                                   >  SRR4302540.8300264/1‑94 (MQ=60)
                                                             ACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGTAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTT                                  >  SRR4302540.3149395/1‑94 (MQ=60)
                                                             ACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTT                                  <  SRR4302540.2519676/94‑1 (MQ=60)
                                                             ACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTT                                  >  SRR4302540.4042796/1‑94 (MQ=60)
                                                             ACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTT                                  >  SRR4302540.5675876/1‑94 (MQ=60)
                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                 >  SRR4302540.9669370/1‑94 (MQ=60)
                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                 <  SRR4302540.6853669/94‑1 (MQ=60)
                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                 <  SRR4302540.6658633/94‑1 (MQ=60)
                                                               AACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTAT                                >  SRR4302540.9723284/1‑94 (MQ=60)
                                                               AACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTAT                                <  SRR4302540.1593120/94‑1 (MQ=60)
                                                               AACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTAT                                <  SRR4302540.9450241/94‑1 (MQ=60)
                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                               <  SRR4302540.7326905/94‑1 (MQ=60)
                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                               <  SRR4302540.8995547/94‑1 (MQ=60)
                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                               <  SRR4302540.4233325/94‑1 (MQ=60)
                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                               >  SRR4302540.3916163/1‑94 (MQ=60)
                                                                 CATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAA                              <  SRR4302540.8066600/94‑1 (MQ=60)
                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGTAATTCACCGACTATTACAACCTCGCTTATAAC                             >  SRR4302540.310171/1‑94 (MQ=60)
                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAC                             <  SRR4302540.10073979/94‑1 (MQ=60)
                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAC                             >  SRR4302540.8596188/1‑94 (MQ=60)
                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAC                             >  SRR4302540.7234733/1‑94 (MQ=60)
                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAC                             <  SRR4302540.6915204/94‑1 (MQ=60)
                                                                   CACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACA                            <  SRR4302540.6636309/94‑1 (MQ=60)
                                                                   AACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACA                            <  SRR4302540.6899673/94‑1 (MQ=60)
                                                                    ACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAA                           >  SRR4302540.412424/1‑94 (MQ=60)
                                                                     CAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAGCAAA                          >  SRR4302540.9506739/1‑94 (MQ=60)
                                                                     CAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAA                          >  SRR4302540.7546494/1‑94 (MQ=60)
                                                                     CAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAA                          <  SRR4302540.8717823/94‑1 (MQ=60)
                                                                     CAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAA                          >  SRR4302540.10137406/1‑94 (MQ=60)
                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                         <  SRR4302540.1682930/94‑1 (MQ=60)
                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                         <  SRR4302540.4749388/94‑1 (MQ=60)
                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                         <  SRR4302540.2880400/94‑1 (MQ=60)
                                                                       GTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACG                        >  SRR4302540.1215618/1‑94 (MQ=60)
                                                                       GTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACG                        >  SRR4302540.71729/1‑94 (MQ=60)
                                                                         CGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCG                      >  SRR4302540.7481638/1‑94 (MQ=60)
                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                     <  SRR4302540.3104120/94‑1 (MQ=60)
                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                     <  SRR4302540.5561202/94‑1 (MQ=60)
                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                     <  SRR4302540.5561198/94‑1 (MQ=60)
                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                     <  SRR4302540.8065148/94‑1 (MQ=60)
                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTCATAACACACGCGG                     >  SRR4302540.3926334/1‑94 (MQ=60)
                                                                           AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGG                    >  SRR4302540.6331769/1‑94 (MQ=60)
                                                                           AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGG                    <  SRR4302540.5602574/94‑1 (MQ=60)
                                                                           AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGG                    >  SRR4302540.6648081/1‑94 (MQ=60)
                                                                              GAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAA                 <  SRR4302540.9825118/94‑1 (MQ=60)
                                                                               AATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAT                >  SRR4302540.6436552/1‑94 (MQ=60)
                                                                                ATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATT               >  SRR4302540.5645523/1‑94 (MQ=60)
                                                                                ATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATT               >  SRR4302540.4152769/1‑94 (MQ=60)
                                                                                 TGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTA              <  SRR4302540.9139005/94‑1 (MQ=60)
                                                                                  GGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAC             <  SRR4302540.10048875/94‑1 (MQ=60)
                                                                                   GCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACA            <  SRR4302540.3084841/94‑1 (MQ=60)
                                                                                   GCGACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACA            <  SRR4302540.7302833/94‑1 (MQ=60)
                                                                                    CTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAA           <  SRR4302540.9033945/94‑1 (MQ=60)
                                                                                    CTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAA           >  SRR4302540.4286064/1‑94 (MQ=60)
                                                                                    CTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAA           <  SRR4302540.1616668/94‑1 (MQ=60)
                                                                                     TACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAC          >  SRR4302540.1658605/1‑94 (MQ=60)
                                                                                      ACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACT         >  SRR4302540.9947620/1‑94 (MQ=60)
                                                                                       CAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTC        >  SRR4302540.9486413/1‑94 (MQ=60)
                                                                                        AACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCA       <  SRR4302540.8515766/94‑1 (MQ=60)
                                                                                        AACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCA       >  SRR4302540.3051596/1‑94 (MQ=60)
                                                                                        AACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCA       >  SRR4302540.2671566/1‑94 (MQ=60)
                                                                                        AACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCA       >  SRR4302540.1931189/1‑94 (MQ=60)
                                                                                         ACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC      >  SRR4302540.1518003/1‑94 (MQ=60)
                                                                                         ACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC‑ACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC     <  SRR4302540.6452407/94‑1 (MQ=60)
                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC     <  SRR4302540.8899658/94‑1 (MQ=60)
                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC     <  SRR4302540.5125538/94‑1 (MQ=60)
                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC     >  SRR4302540.7508524/1‑94 (MQ=60)
                                                                                           ATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCG    <  SRR4302540.9586167/94‑1 (MQ=60)
                                                                                           ATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCG    <  SRR4302540.4174869/94‑1 (MQ=60)
                                                                                           ATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCG    <  SRR4302540.8743258/94‑1 (MQ=60)
                                                                                           ATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCG    <  SRR4302540.5620902/94‑1 (MQ=60)
                                                                                             CGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTT  >  SRR4302540.3212296/1‑94 (MQ=60)
                                                                                             |                                                                                             
CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTT  >  NC_000913/4544077‑4544263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑