Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,170	T→C	I502I (ATT→ATC)	fimD →	FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,170	0	T	C	99.6%	793.0 / NA	243	I502I (ATT→ATC)	fimD	FimD
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/111); minor base A (1/0); total (132/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  2:891017/1‑93 (MQ=255)

 aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaa                                                                                        >  1:5271471/1‑93 (MQ=255)

  atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaaa                                                                                       >  2:3919011/1‑93 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGCATGGCTAGAACATCGAAac                                                                                      >  1:2569209/1‑93 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      <  2:168428/93‑1 (MQ=255)

     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacac                                                                                    >  2:714634/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATTGCTACAACATCGAAACACAg                                                                                  >  2:6294717/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  >  1:503744/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  1:1419816/93‑1 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  2:1109358/93‑1 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  1:2475304/93‑1 (MQ=255)

        aGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAgg                                                                                 <  2:4876477/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  1:4630638/1‑93 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                <  2:1901434/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  2:3977773/1‑93 (MQ=255)

           tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACg                                                                              <  1:2615883/93‑1 (MQ=255)

            aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACgg                                                                             >  1:3765361/1‑93 (MQ=255)

            aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACgg                                                                             >  1:4150033/1‑93 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:1923080/93‑1 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:4102798/93‑1 (MQ=255)

               tggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGt                                                                          <  1:703694/92‑1 (MQ=255)

                  ttACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAt                                                                       >  2:2616114/1‑93 (MQ=255)

                    aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTc                                                                     >  1:4107422/1‑93 (MQ=255)

                     ccGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCa                                                                    <  2:2347104/93‑1 (MQ=255)

                      cGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAg                                                                   <  2:3306565/93‑1 (MQ=255)

                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAAGACGGAGTTATTCAgg                                                                  >  2:5826228/1‑93 (MQ=255)

                        ttATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGt                                                                 <  1:1233887/93‑1 (MQ=255)

                         tATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGtt                                                                >  1:1610247/1‑93 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              <  1:1000714/93‑1 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              >  1:5159406/1‑93 (MQ=255)

                             cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGc                                                            >  1:5426609/1‑93 (MQ=255)

                                ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGa                                                         >  1:1486542/1‑93 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACTGAGGTATTCAGGTTAAGCCGaaa                                                       >  2:2199546/1‑93 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       <  2:918865/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      <  1:4732050/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      >  1:4073989/1‑93 (MQ=255)

                                    cGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaact                                                     >  1:2011336/1‑91 (MQ=255)

                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    >  1:4933701/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  1:2132126/93‑1 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  1:5594838/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  1:2736070/93‑1 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  2:1418805/1‑93 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               <  1:5850160/93‑1 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATAGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTTACTGa                                               >  2:3072066/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:1091248/93‑1 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              >  2:4606534/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:5323577/93‑1 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACATAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  2:6215975/1‑93 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  2:1741607/1‑93 (MQ=255)

                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        <  2:1990806/93‑1 (MQ=255)

                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        <  1:3315294/93‑1 (MQ=255)

                                                  tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACa                                       >  2:4414785/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      >  1:2285737/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      <  2:1770692/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  1:907913/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  1:3777888/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  2:5001280/93‑1 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:3738129/93‑1 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:4745106/93‑1 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  >  1:3027296/1‑93 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  <  2:1291071/93‑1 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                <  2:2949021/93‑1 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  1:1840192/1‑93 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:1851969/1‑93 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:6247339/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            <  2:5773018/93‑1 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  1:1712686/1‑93 (MQ=255)

                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           <  2:3694376/93‑1 (MQ=255)

                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGCTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           >  2:2862303/1‑93 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          >  2:181397/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:4348753/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:1050063/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGGTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:5407993/1‑93 (MQ=255)

                                                                 aCAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACa                        >  1:4888072/1‑93 (MQ=255)

                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       >  1:2528900/1‑93 (MQ=255)

                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       >  1:1957768/1‑93 (MQ=255)

                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      <  1:973225/93‑1 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     >  1:3908831/1‑93 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     <  2:5537958/93‑1 (MQ=255)

                                                                     tCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                    <  1:421968/93‑1 (MQ=255)

                                                                       gaatgaatGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgcg                  <  2:1565183/93‑1 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:3456183/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  1:2214103/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:4201107/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 <  2:1253733/93‑1 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  1:2986521/1‑93 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCggg                <  2:3418645/93‑1 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCggg                <  2:5068327/93‑1 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAACTCGCTTATAACAAACGCggg                <  2:4561716/93‑1 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  1:6160798/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  2:5740334/1‑93 (MQ=255)

                                                                           gaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaa              <  2:704515/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  1:4561292/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  1:3628108/93‑1 (MQ=255)

                                                                              tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           <  2:2625855/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:2834625/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:375040/93‑1 (MQ=255)

                                                                                 cTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACa        >  1:2937399/1‑93 (MQ=255)

                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  >  2:2796333/1‑93 (MQ=255)

                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  <  1:5672467/93‑1 (MQ=255)

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGT  >  NC_000913/4544077‑4544262

CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATC                                                                                              >  SRR4302533.2654186/1‑94 (MQ=60)

  CGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGA                                                                                            >  SRR4302533.2375951/1‑94 (MQ=60)

   GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAA                                                                                           <  SRR4302533.2110140/94‑1 (MQ=60)

   GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAA                                                                                           >  SRR4302533.5303086/1‑94 (MQ=60)

   GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAA                                                                                           >  SRR4302533.2539692/1‑94 (MQ=60)

     ATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGCATGGCTAGAACATCGAAAC                                                                                         >  SRR4302533.2585019/1‑94 (MQ=60)

      TATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACA                                                                                        <  SRR4302533.2223821/94‑1 (MQ=60)

      TATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACA                                                                                        <  SRR4302533.3069052/94‑1 (MQ=60)

        TTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACA                                                                                      <  SRR4302533.6239331/94‑1 (MQ=60)

         TCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAG                                                                                     <  SRR4302533.2602856/94‑1 (MQ=60)

         TCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAG                                                                                     >  SRR4302533.506733/1‑94 (MQ=60)

          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                    <  SRR4302533.2490571/94‑1 (MQ=60)

          CAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGG                                                                                    <  SRR4302533.1428406/94‑1 (MQ=60)

           AGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGA                                                                                   >  SRR4302533.4658702/1‑94 (MQ=60)

            GTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGAC                                                                                  <  SRR4302533.4154962/94‑1 (MQ=60)

              TAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGG                                                                                >  SRR4302533.3788445/1‑94 (MQ=60)

              TAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGG                                                                                >  SRR4302533.4175174/1‑94 (MQ=60)

              TAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGG                                                                                <  SRR4302533.2631940/94‑1 (MQ=60)

                  TGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTT                                                                            <  SRR4302533.707854/94‑1 (MQ=60)

                      TACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTC                                                                        >  SRR4302533.4132395/1‑94 (MQ=60)

                       ACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCA                                                                       >  SRR4302533.2308270/1‑94 (MQ=60)

                        CCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAG                                                                      <  SRR4302533.4154518/94‑1 (MQ=60)

                           TTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTT                                                                   >  SRR4302533.1620011/1‑94 (MQ=60)

                           TTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTT                                                                   <  SRR4302533.1241267/94‑1 (MQ=60)

                            TATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTA                                                                  <  SRR4302533.651442/94‑1 (MQ=60)

                             ATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAA                                                                 >  SRR4302533.5190547/1‑94 (MQ=60)

                              TTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAG                                                                <  SRR4302533.925141/94‑1 (MQ=60)

                              TTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAG                                                                <  SRR4302533.1006556/94‑1 (MQ=60)

                               TCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGC                                                               >  SRR4302533.5459289/1‑94 (MQ=60)

                                  ACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGA                                                            >  SRR4302533.1495509/1‑94 (MQ=60)

                                   CCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGCAACACAGGACGGAGTTATTCAGGTTAAGCCGAA                                                           >  SRR4302533.4381554/1‑94 (MQ=60)

                                     AGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAT                                                         >  SRR4302533.4098792/1‑94 (MQ=60)

                                      GCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATT                                                        <  SRR4302533.4760738/94‑1 (MQ=60)

                                      GCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAACT                                                        >  SRR4302533.2023728/1‑94 (MQ=60)

                                       CGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTC                                                       <  SRR4302533.5220760/94‑1 (MQ=60)

                                       CGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTC                                                       >  SRR4302533.4963576/1‑94 (MQ=60)

                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCC                                                      >  SRR4302533.3788739/1‑94 (MQ=60)

                                        GGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCA                                                      >  SRR4302533.1521091/1‑94 (MQ=60)

                                         GATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAC                                                     >  SRR4302533.500713/1‑94 (MQ=60)

                                         GATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAC                                                     >  SRR4302533.5628638/1‑94 (MQ=60)

                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGATTGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                    <  SRR4302533.3686888/94‑1 (MQ=60)

                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                    <  SRR4302533.1809461/94‑1 (MQ=60)

                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                    <  SRR4302533.2752853/94‑1 (MQ=60)

                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                    >  SRR4302533.4951221/1‑94 (MQ=60)

                                          ATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACC                                                    <  SRR4302533.2145274/94‑1 (MQ=60)

                                           TATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCG                                                   <  SRR4302533.2563094/94‑1 (MQ=60)

                                           TATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCG                                                   <  SRR4302533.1144466/94‑1 (MQ=60)

                                            ATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGA                                                  >  SRR4302533.5682910/1‑94 (MQ=60)

                                            ATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGA                                                  >  SRR4302533.1388010/1‑94 (MQ=60)

                                            ATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGA                                                  <  SRR4302533.5084427/94‑1 (MQ=60)

                                             TTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAC                                                 <  SRR4302533.4788296/94‑1 (MQ=60)

                                             TTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAC                                                 <  SRR4302533.5885532/94‑1 (MQ=60)

                                              TTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACT                                                <  SRR4302533.5355480/94‑1 (MQ=60)

                                              TTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACT                                                <  SRR4302533.1097695/94‑1 (MQ=60)

                                                TAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTAT                                              <  SRR4302533.1483962/94‑1 (MQ=60)

                                                  ATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTA                                            >  SRR4302533.5382022/1‑94 (MQ=60)

                                                    TTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                          <  SRR4302533.3335763/94‑1 (MQ=60)

                                                    TTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACA                                          <  SRR4302533.5351798/94‑1 (MQ=60)

                                                     TCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAA                                         >  SRR4302533.2299885/1‑94 (MQ=60)

                                                     TCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAA                                         <  SRR4302533.4942893/94‑1 (MQ=60)

                                                     TCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAA                                         <  SRR4302533.3707130/94‑1 (MQ=60)

                                                      CGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAC                                        <  SRR4302533.1852170/94‑1 (MQ=60)

                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                       <  SRR4302533.3801036/94‑1 (MQ=60)

                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                       <  SRR4302533.913238/94‑1 (MQ=60)

                                                       GCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACC                                       <  SRR4302533.198269/94‑1 (MQ=60)

                                                         TGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTC                                     >  SRR4302533.3045822/1‑94 (MQ=60)

                                                          GATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCG                                    <  SRR4302533.918842/94‑1 (MQ=60)

                                                           ATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGC                                   >  SRR4302533.1851412/1‑94 (MQ=60)

                                                             ACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTT                                 >  SRR4302533.6293666/1‑94 (MQ=60)

                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                >  SRR4302533.1863261/1‑94 (MQ=60)

                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                >  SRR4302533.6284725/1‑94 (MQ=60)

                                                              CAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTA                                >  SRR4302533.594949/1‑94 (MQ=60)

                                                               AACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTAT                               >  SRR4302533.1723063/1‑94 (MQ=60)

                                                               AACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTAT                               >  SRR4302533.2682055/1‑94 (MQ=60)

                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                              >  SRR4302533.1037253/1‑94 (MQ=60)

                                                                ACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATA                              >  SRR4302533.3812440/1‑94 (MQ=60)

                                                                 CATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAA                             <  SRR4302533.4236128/94‑1 (MQ=60)

                                                                 CATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAA                             <  SRR4302533.4580111/94‑1 (MQ=60)

                                                                 CATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAA                             <  SRR4302533.1959849/94‑1 (MQ=60)

                                                                 CATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAA                             >  SRR4302533.6195653/1‑94 (MQ=60)

                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAC                            <  SRR4302533.2277567/94‑1 (MQ=60)

                                                                  ATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTAACCGACTATTACAACCTCGCTTATAAA                            >  SRR4302533.2977080/1‑94 (MQ=60)

                                                                   TACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACA                           >  SRR4302533.4917689/1‑94 (MQ=60)

                                                                    ACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAA                          >  SRR4302533.1969717/1‑94 (MQ=60)

                                                                    ACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAA                          >  SRR4302533.2544470/1‑94 (MQ=60)

                                                                     CAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAA                         >  SRR4302533.881268/1‑94 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        <  SRR4302533.978939/94‑1 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        <  SRR4302533.2070643/94‑1 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        >  SRR4302533.5713937/1‑94 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        <  SRR4302533.2995113/94‑1 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        >  SRR4302533.3038701/1‑94 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        >  SRR4302533.461771/1‑94 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        >  SRR4302533.3932705/1‑94 (MQ=60)

                                                                      AGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTAAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAC                        <  SRR4302533.3410611/94‑1 (MQ=60)

                                                                        TCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGC                      <  SRR4302533.424492/94‑1 (MQ=60)

                                                                         CGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCG                     >  SRR4302533.1882751/1‑94 (MQ=60)

                                                                         CGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCG                     >  SRR4302533.5535190/1‑94 (MQ=60)

                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                    >  SRR4302533.4019/1‑94 (MQ=60)

                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                    >  SRR4302533.1774567/1‑94 (MQ=60)

                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                    >  SRR4302533.2227766/1‑94 (MQ=60)

                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                    >  SRR4302533.3004791/1‑94 (MQ=60)

                                                                          GAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATACAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGG                    >  SRR4302533.1546569/1‑94 (MQ=60)

                                                                           AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGG                   <  SRR4302533.4662657/94‑1 (MQ=60)

                                                                            ATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGA                  >  SRR4302533.6197848/1‑94 (MQ=60)

                                                                             TGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAA                 >  SRR4302533.5190018/1‑94 (MQ=60)

                                                                             AGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAA                 <  SRR4302533.5683797/94‑1 (MQ=60)

                                                                              GAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAA                >  SRR4302533.5369275/1‑94 (MQ=60)

                                                                               AATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAT               <  SRR4302533.3650432/94‑1 (MQ=60)

                                                                               AATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAT               <  SRR4302533.4588955/94‑1 (MQ=60)

                                                                               AATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAT               <  SRR4302533.1701343/94‑1 (MQ=60)

                                                                                 TGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTA             >  SRR4302533.4741647/1‑94 (MQ=60)

                                                                                 TGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTA             <  SRR4302533.2546683/94‑1 (MQ=60)

                                                                                   GCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACA           >  SRR4302533.2955352/1‑94 (MQ=60)

                                                                                    CTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAA          >  SRR4302533.1402072/1‑94 (MQ=60)

                                                                                     TACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAC         >  SRR4302533.4038518/1‑94 (MQ=60)

                                                                                     TACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAC         <  SRR4302533.332622/94‑1 (MQ=60)

                                                                                     TACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAAC         >  SRR4302533.2829428/1‑94 (MQ=60)

                                                                                        AACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAGCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCA      >  SRR4302533.4112078/1‑94 (MQ=60)

                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC    <  SRR4302533.2752093/94‑1 (MQ=60)

                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC    <  SRR4302533.5706727/94‑1 (MQ=60)

                                                                                          CATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACC    >  SRR4302533.3170668/1‑94 (MQ=60)

                                                                                            TCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGT  >  SRR4302533.6021812/1‑94 (MQ=60)

CACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGT  >  NC_000913/4544077‑4544262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`