Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,123,585 +G coding (953/2286 nt) glcF ← glycolate oxidase iron‑sulfur subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,123,5821.G99.5% 1627.2 / ‑1.8 427coding (956/2286 nt)glcFglycolate oxidase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (226/199);  total (226/201)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 487 total aligned reads displayed.

CAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCG  >  NC_000913/3123487‑3123672
                                                                                                |                                                                                          
cAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGc                                                                                             >  1:10032418/1‑96 (MQ=255)
 aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                           >  1:792108/1‑96 (MQ=255)
 aaaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑g                                                                                           <  2:8257184/96‑1 (MQ=255)
  aaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑gg                                                                                          >  2:4791960/1‑96 (MQ=255)
     ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggc                                                                                       >  1:3126431/1‑94 (MQ=255)
     ttCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggc                                                                                       >  2:4134160/1‑94 (MQ=255)
      tCCGCGTACATGCGACCGGGGGTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggca                                                                                      >  1:8207522/1‑93 (MQ=255)
       ccGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑ggggcaa                                                                                     >  2:6338047/1‑92 (MQ=255)
         gcgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAg                                                                                    >  1:14143674/1‑96 (MQ=255)
          cgTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGc                                                                                   >  2:1493372/1‑96 (MQ=255)
          cgTACATGCCACCGGGGTTAAACAAGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGTTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGc                                                                                   <  1:6315819/96‑1 (MQ=255)
           gTACAGGCGCCCGGGGTTAAACTCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGct                                                                                  <  1:11348522/96‑2 (MQ=255)
               aTGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGccacca                                                                              >  2:300976/1‑96 (MQ=255)
                tGCGACCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAt                                                                             <  1:11973713/96‑1 (MQ=255)
                 gCGACCGGGGTTAAACCCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtc                                                                            <  2:3122983/96‑1 (MQ=255)
                  cGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtct                                                                           >  1:5654054/1‑96 (MQ=255)
                   gACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATGGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCGACCAtctg                                                                          >  1:4084883/1‑95 (MQ=255)
                   gACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtctc                                                                          <  1:12099654/96‑1 (MQ=255)
                   gACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtctc                                                                          >  1:10860902/1‑96 (MQ=255)
                   gACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCAtctc                                                                          >  2:8976071/1‑96 (MQ=255)
                    cccGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTcc                                                                         <  2:13507465/95‑1 (MQ=255)
                     cgggggTTAAACACGCCGCAAGGGCCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                        >  1:11822734/3‑96 (MQ=255)
                     ccGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCg                                                                        <  2:6177485/96‑1 (MQ=255)
                      cGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCgg                                                                       <  1:9401101/96‑1 (MQ=255)
                        gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCa                                                                     >  2:5981448/1‑96 (MQ=255)
                          tttAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACt                                                                   <  1:12361126/95‑1 (MQ=255)
                             aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTata                                                                <  1:10668943/96‑3 (MQ=255)
                             aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTaaa                                                                >  1:9250374/1‑96 (MQ=255)
                             aaaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTaaa                                                                >  1:7640215/1‑96 (MQ=255)
                             aaaCACGCCGCAAGGGTCGAGCTGCTGTGTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTaaa                                                                >  2:13902551/1‑96 (MQ=255)
                              aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAg                                                               >  1:6636664/1‑96 (MQ=255)
                              aaCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAg                                                               >  2:9726087/1‑96 (MQ=255)
                                cacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCg                                                             >  2:7138050/1‑96 (MQ=255)
                                 acGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCgg                                                            <  1:9880104/96‑1 (MQ=255)
                                 acGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCgg                                                            >  2:7994803/1‑96 (MQ=255)
                                   gccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGt                                                          <  1:7785359/96‑1 (MQ=255)
                                    ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGGAAAGCCACCATCTCCGGCACTACAGCggacc                                                         >  1:3377712/1‑93 (MQ=255)
                                    ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTc                                                         <  2:13076389/96‑1 (MQ=255)
                                    ccgcAAGGGTCGAGCGGCTGTTTAAGCTGCTGGTGATAGCGAAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTc                                                         <  1:8771248/96‑1 (MQ=255)
                                      gcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGGGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGc                                                       >  2:11701127/1‑96 (MQ=255)
                                       cAAGGGTGTAGCTGCCGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCa                                                      <  1:6549998/96‑1 (MQ=255)
                                       cAAGGGTCGAGCTGCTGTTTAAGCTGCTGGGGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCa                                                      >  2:14030655/1‑96 (MQ=255)
                                        aaGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAt                                                     >  2:5865650/1‑96 (MQ=255)
                                            gTCGAGCTGCTGGTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                                 >  1:6169131/1‑96 (MQ=255)
                                            gTCGAGCTGCGGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAcc                                                 <  2:1249083/96‑1 (MQ=255)
                                             tgcaGCTGCTGTTTAAGCTGCTGGTCATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGCCCg                                                <  1:9112120/93‑1 (MQ=255)
                                             tCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCg                                                <  1:5859146/96‑1 (MQ=255)
                                              ggAGCTGCTGTTTCAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc                                               >  1:13858918/2‑96 (MQ=255)
                                              cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGTACTAAAGCGGGTCGCATGACCGc                                               >  1:12565190/1‑96 (MQ=255)
                                              cGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGc                                               >  1:70648/1‑96 (MQ=255)
                                               gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCggc                                              >  1:10433057/1‑94 (MQ=255)
                                               gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGcc                                              >  2:7881757/1‑96 (MQ=255)
                                                 gctgctGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAg                                            >  1:2202058/1‑96 (MQ=255)
                                                  ctgctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGc                                           >  1:7340770/1‑96 (MQ=255)
                                                    gctgTTTAAGCTGCTGGTGGTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGGCCCATGACCGCCAGCGt                                         >  1:2781193/1‑96 (MQ=255)
                                                      tgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCCCCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTg                                       <  1:2672743/96‑1 (MQ=255)
                                                      tgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTg                                       <  2:8162356/96‑1 (MQ=255)
                                                        tttAAGCTGCTGTTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCCCCCTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                     <  2:13269685/96‑1 (MQ=255)
                                                        tttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCg                                     <  2:683510/96‑1 (MQ=255)
                                                         ttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                    >  2:5194723/1‑96 (MQ=255)
                                                         ttAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCgg                                    <  2:10292176/96‑1 (MQ=255)
                                                          tAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                   <  2:879074/96‑1 (MQ=255)
                                                          tAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                   >  2:4406303/1‑96 (MQ=255)
                                                          tAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                   <  1:8390435/96‑1 (MQ=255)
                                                          tAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCggg                                   <  2:7633582/96‑1 (MQ=255)
                                                           aaGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgc                                  >  1:11635416/1‑96 (MQ=255)
                                                            aGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcg                                 <  2:11993680/96‑1 (MQ=255)
                                                               tgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatg                              <  1:503786/96‑1 (MQ=255)
                                                                gctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgc                             >  2:12304150/1‑96 (MQ=255)
                                                                 ctgGTGATTGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                            >  1:12973792/1‑96 (MQ=255)
                                                                 ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                            >  2:12678458/1‑96 (MQ=255)
                                                                 ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcg                            <  2:3844101/96‑1 (MQ=255)
                                                                  tgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGgcgatgcga                           >  2:6935579/1‑96 (MQ=255)
                                                                    gTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGcgatgcgatg                         >  2:2568009/1‑96 (MQ=255)
                                                                     tGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg                        <  1:747885/96‑1 (MQ=255)
                                                                     tGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATgg                        <  2:535861/96‑1 (MQ=255)
                                                                       aTAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAt                      <  2:5068337/96‑1 (MQ=255)
                                                                         aGCGAAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGAttt                    <  2:3501103/96‑1 (MQ=255)
                                                                             gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAtt                <  1:10307576/96‑1 (MQ=255)
                                                                             gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAtt                <  2:11024074/96‑1 (MQ=255)
                                                                              aaTAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTg               >  2:9330619/1‑96 (MQ=255)
                                                                               tcaaaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGt              <  1:833503/94‑1 (MQ=255)
                                                                                tAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTc             >  1:4749733/1‑96 (MQ=255)
                                                                                  aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt           >  2:4504825/1‑96 (MQ=255)
                                                                                  aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt           >  1:5378929/1‑96 (MQ=255)
                                                                                  aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt           <  2:2417260/96‑1 (MQ=255)
                                                                                  aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt           >  1:44773/1‑96 (MQ=255)
                                                                                  aaGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCt           <  1:4406303/96‑1 (MQ=255)
                                                                                   gggAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc          <  1:6866329/95‑1 (MQ=255)
                                                                                   aagAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc          >  1:14319197/3‑96 (MQ=255)
                                                                                   aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTc          <  1:1278351/96‑1 (MQ=255)
                                                                                   aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGAGGGGTTTGATTGGCCTc          >  1:5262656/1‑96 (MQ=255)
                                                                                    ggAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg         >  1:383442/1‑96 (MQ=255)
                                                                                    ggAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCg         <  2:10494838/96‑1 (MQ=255)
                                                                                     gAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCgg        >  2:10773952/1‑96 (MQ=255)
                                                                                      aGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGc       <  2:11478468/96‑1 (MQ=255)
                                                                                      aGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGc       >  1:13240093/1‑96 (MQ=255)
                                                                                         cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGt    <  1:1853632/96‑1 (MQ=255)
                                                                                         cGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGt    <  2:7491424/96‑1 (MQ=255)
                                                                                          gATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTc   <  2:11902666/96‑1 (MQ=255)
                                                                                                |                                                                                          
CAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCG  >  NC_000913/3123487‑3123672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 305 total aligned reads displayed.
CTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCG  >  NC_000913/3123485‑3123685
                                                                                                      |                                                                                                     
 TCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GG‑G                                                                                                     >  SRR4302518.285910/1‑101 (MQ=60)
 TCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GG‑G                                                                                                     >  SRR4302518.4810113/1‑101 (MQ=60)
   nnngtcCCGCGTACATGCGCCGGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgca                                                                                                    <  SRR4302518.8315156/95‑4 (MQ=60)
   cattttccgcgttcgtgccACCGGGGTTAAACCCGCCGCAAGGGTCGGGCTGCTGTTTAAGCTGCGGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgca                                                                                                    <  SRR4302518.3727147/82‑4 (MQ=60)
   TAAGTTCCGCGTATATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGGGCCGATAGC‑G‑GG‑NNN                                                                                                   >  SRR4302518.11923422/1‑101 (MQ=60)
   NNNGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgca                                                                                                    <  SRR4302518.10944914/101‑4 (MQ=60)
   AAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgca                                                                                                    >  SRR4302518.822860/1‑98 (MQ=60)
   AAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GG‑NNN                                                                                                   >  SRR4302518.13246446/1‑101 (MQ=60)
   AAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGACAGC‑G‑GG‑NNN                                                                                                   >  SRR4302518.14921598/1‑101 (MQ=60)
     nnngccgcgtacgtgctgccggtGTTAAACCCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAA                                                                                                  <  SRR4302518.12990673/78‑1 (MQ=60)
       TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGC                                                                                                >  SRR4302518.6924835/1‑101 (MQ=60)
       TTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGC                                                                                                >  SRR4302518.3272849/1‑101 (MQ=60)
       TTCCCCGTACATGCCACCGGGGTTAAACAAGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGTTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGC                                                                                                <  SRR4302518.6637804/101‑1 (MQ=60)
        TCCGCGTACATGCGACCGGGGGTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgcaaannn                                                                                               >  SRR4302518.8613074/1‑93 (MQ=60)
        NNNGCGTACAGGCGCCCGGGGTTAAACTCGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GGgcaaagct                                                                                               <  SRR4302518.11880408/101‑9 (MQ=60)
         CCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGAAGCCGATAGC‑G‑GGggcaagnnn                                                                                              >  SRR4302518.9102770/1‑92 (MQ=60)
           GGGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCNNN                                                                                            >  SRR4302518.11629214/1‑101 (MQ=60)
           GGGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCNNN                                                                                            >  SRR4302518.14950222/1‑101 (MQ=60)
           GCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACC                                                                                            >  SRR4302518.1375685/1‑101 (MQ=60)
           GCGTACATGCGACCGGGGGTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCNNN                                                                                            >  SRR4302518.12699276/1‑101 (MQ=60)
           GAGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCNNN                                                                                            >  SRR4302518.11204324/1‑101 (MQ=60)
            CGTACATGCGCCCGGGGTTAAACACGCCGCAAGGGTCGAGCTCCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCA                                                                                           <  SRR4302518.5943427/101‑1 (MQ=60)
             GTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCAT                                                                                          >  SRR4302518.6954650/1‑101 (MQ=60)
             GTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCAT                                                                                          <  SRR4302518.200019/101‑1 (MQ=60)
             GTACATGCGACCGGGGGTAAACACGCCGCAAGGGGCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAGTAAAGGAGCCGATAGC‑G‑GGgcacagccacnnn                                                                                          >  SRR4302518.9864644/1‑88 (MQ=60)
              TACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATC                                                                                         >  SRR4302518.711672/1‑101 (MQ=60)
              TACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATC                                                                                         >  SRR4302518.4970160/1‑101 (MQ=60)
               nnnttcgtccgggctaAAACACGCCGAAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAACGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCT                                                                                        <  SRR4302518.12724896/85‑1 (MQ=60)
               ACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCT                                                                                        >  SRR4302518.473279/1‑101 (MQ=60)
                NNNGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTC                                                                                       <  SRR4302518.7967744/101‑1 (MQ=60)
                NNNGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTC                                                                                       <  SRR4302518.12674966/101‑1 (MQ=60)
                 ctccgaccggggttaaccaacctgcaagtgtagcgctgcatTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCC                                                                                      <  SRR4302518.649147/60‑1 (MQ=60)
                 NNNCGACAGGGGTAAAACACGTCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCC                                                                                      <  SRR4302518.10824558/101‑1 (MQ=60)
                   ccatCCGGGGTTAAACAATACGCAAGGGTCAAGCTGCTGTTTAAACTGCTGGTGGTAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CATAGCCACCATCTCCGG                                                                                    <  SRR4302518.6687841/97‑1 (MQ=60)
                   NNNACCGGGGTTAACCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGG                                                                                    <  SRR4302518.9855731/101‑1 (MQ=60)
                   GCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCNNN                                                                                    >  SRR4302518.8319309/1‑101 (MQ=60)
                    CGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGC                                                                                   >  SRR4302518.5941076/1‑101 (MQ=60)
                    CGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCAGGC                                                                                   >  SRR4302518.1616622/1‑101 (MQ=60)
                     GACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGNNN                                                                                  >  SRR4302518.11370510/1‑101 (MQ=60)
                     GACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑GCGG‑CAAAGCCACCATCTCCGNNN                                                                                  >  SRR4302518.14816098/1‑101 (MQ=60)
                      GCCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCAC                                                                                 <  SRR4302518.4081613/101‑1 (MQ=60)
                      CCCGGTGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGAAAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCAC                                                                                 <  SRR4302518.1080779/101‑1 (MQ=60)
                      ACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGNNN                                                                                 >  SRR4302518.10238794/1‑101 (MQ=60)
                       nnnggtttaacCACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACT                                                                                <  SRR4302518.12944425/90‑1 (MQ=60)
                       CGGGGGTTAAACACGCCGCAAGGGCCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCNNN                                                                                >  SRR4302518.12385281/1‑101 (MQ=60)
                        CGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTA                                                                               >  SRR4302518.5467123/1‑101 (MQ=60)
                          GGGTTAAACACGCCGCAAGGGTAGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTNNN                                                                             >  SRR4302518.7358799/1‑101 (MQ=60)
                           NNNTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAG                                                                            <  SRR4302518.9680926/101‑1 (MQ=60)
                            nnnagacaccgcgcagGGGTCGAGCTGCTGTTTAAGTTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGC                                                                           <  SRR4302518.7646533/85‑1 (MQ=60)
                            GTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGC                                                                           <  SRR4302518.6577116/101‑1 (MQ=60)
                              NNNACACGCCGCACGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGG                                                                         <  SRR4302518.8640251/101‑1 (MQ=60)
                              NNNACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGG                                                                         <  SRR4302518.10351927/101‑1 (MQ=60)
                               AAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCNNN                                                                        >  SRR4302518.8029479/1‑101 (MQ=60)
                               AAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGG                                                                        >  SRR4302518.913073/1‑101 (MQ=60)
                                 ACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTC                                                                      >  SRR4302518.5840170/1‑101 (MQ=60)
                                  CCCGCCGCAAGGGTGGAGCTGCTGTTTACGCTGCTGGTGATAGTGGAATAAAGGAGCCGTTAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCG                                                                     <  SRR4302518.1254759/101‑1 (MQ=60)
                                    CGCCGCAGGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCA                                                                   <  SRR4302518.5604376/101‑1 (MQ=60)
                                    CGCCGCAAGGGTGTAGCTGCCGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCA                                                                   <  SRR4302518.6886287/101‑1 (MQ=60)
                                    CGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCNNN                                                                   >  SRR4302518.8156853/1‑101 (MQ=60)
                                    CGCCGCAAGGGTCGAGCTGCTGTTTAAGATGCTGGTGATAGCGGAATAAAGGAGCCGATAGCTA‑GG‑CAAAGCCACCAGCTCCGGCACTAAAGCGGaccgct                                                                   >  SRR4302518.4925922/1‑95 (MQ=60)
                                    CGCAGCAAGGGTAGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCA                                                                   <  SRR4302518.1254176/101‑1 (MQ=60)
                                     NNNGCAGGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAT                                                                  <  SRR4302518.9781686/101‑1 (MQ=60)
                                     NNNGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAT                                                                  <  SRR4302518.12020667/101‑1 (MQ=60)
                                     NCAGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAT                                                                  <  SRR4302518.2803145/101‑1 (MQ=60)
                                     GCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCAACTCCGGCACTAAAGggggacgnnn                                                                  >  SRR4302518.9698594/1‑91 (MQ=60)
                                      nnncagGGGTCGAGCTGCTGTTTAAGGTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCGCCATCTCCGGCACTAAAGCGGGTCGCATG                                                                 <  SRR4302518.7573948/95‑1 (MQ=60)
                                       nnnaagggcccggtcGCTGTTTAAGCTGCTGGTCAGAGCGGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCCCTAAAGCGGGTCGCATGC                                                                <  SRR4302518.9246683/86‑1 (MQ=60)
                                       NNNAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGA                                                                <  SRR4302518.8198671/101‑1 (MQ=60)
                                        GCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATNNN                                                               >  SRR4302518.8022711/1‑101 (MQ=60)
                                        GCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAC                                                               >  SRR4302518.133493/1‑101 (MQ=60)
                                        GCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGAC                                                               >  SRR4302518.3427330/1‑101 (MQ=60)
                                         NNNGGGCCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                              <  SRR4302518.14765253/101‑1 (MQ=60)
                                         CAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGNNN                                                              >  SRR4302518.13881752/1‑101 (MQ=60)
                                         CAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                              >  SRR4302518.711041/1‑101 (MQ=60)
                                         CAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                              >  SRR4302518.4026049/1‑101 (MQ=60)
                                         CAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACC                                                              >  SRR4302518.4334540/1‑101 (MQ=60)
                                          nnnggtgcAGCTGCTGTTTAAGCTGCTGGTCATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGCCCG                                                             <  SRR4302518.9552017/93‑1 (MQ=60)
                                          AAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGANNN                                                             >  SRR4302518.8113732/1‑101 (MQ=60)
                                          AAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCG                                                             <  SRR4302518.6156005/101‑1 (MQ=60)
                                          AAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCG                                                             >  SRR4302518.1244694/1‑101 (MQ=60)
                                          AAGGATCGAGCTGCTGTTGAAGCTGCTGGTGATAGCGGAGTAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGANNN                                                             >  SRR4302518.13225801/1‑101 (MQ=60)
                                           AGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGC                                                            <  SRR4302518.123026/101‑1 (MQ=60)
                                            nnngcgagctgcgtTTTAAGATGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCA                                                           <  SRR4302518.9084346/87‑1 (MQ=60)
                                            NNNTCGAGCTGCTGTTTAAGCAGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCC                                                           <  SRR4302518.13380281/101‑1 (MQ=60)
                                            GGGTCGGGCGCCTGTTTCAGCTGCTGGTGATAGGGGAATAAAGGAGCCGATAGC‑G‑GGTCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCC                                                           <  SRR4302518.2853781/101‑1 (MQ=60)
                                             GGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCA                                                          >  SRR4302518.6772935/1‑101 (MQ=60)
                                             GGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCA                                                          <  SRR4302518.3453605/101‑1 (MQ=60)
                                              GTCTAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCNNN                                                         >  SRR4302518.14692453/1‑101 (MQ=60)
                                              GTCGAGCTGCTGGTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAG                                                         >  SRR4302518.6483310/1‑101 (MQ=60)
                                               TCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCCCTAAAGCGGGTCGCATGACCGCCAAC                                                        >  SRR4302518.4500144/1‑101 (MQ=60)
                                               NNNAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCCCTAAAGCGGGTCGCATGACCGCCAAC                                                        <  SRR4302518.11036928/101‑1 (MQ=60)
                                               NNNAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGC                                                        <  SRR4302518.9354647/101‑1 (MQ=60)
                                                CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGTACTAAAGCGGGTCGCATGACCGCCANNN                                                       >  SRR4302518.13154542/1‑101 (MQ=60)
                                                CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCG                                                       >  SRR4302518.330619/1‑101 (MQ=60)
                                                CGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCG                                                       >  SRR4302518.73472/1‑101 (MQ=60)
                                                 GAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGNNN                                                      >  SRR4302518.8890660/1‑101 (MQ=60)
                                                  nnntgaTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTT                                                     <  SRR4302518.9703042/95‑1 (MQ=60)
                                                  agcgcctgttgaggcgGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCACGACCGCCAGCGTT                                                     <  SRR4302518.2324070/85‑1 (MQ=60)
                                                  NNNTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTT                                                     <  SRR4302518.12981751/101‑1 (MQ=60)
                                                  AGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCNNN                                                     >  SRR4302518.10956112/1‑101 (MQ=60)
                                                  AGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCNNN                                                     >  SRR4302518.9665771/1‑101 (MQ=60)
                                                   GCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCCCCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTG                                                    <  SRR4302518.2800280/101‑1 (MQ=60)
                                                   GCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTG                                                    >  SRR4302518.6285938/1‑101 (MQ=60)
                                                    CTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTNNN                                                   >  SRR4302518.7722082/1‑101 (MQ=60)
                                                     TGCTGTTTAAGCTGCTGGTGGTAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTCAAGCGGGGCGCATGACCGCCCGCGTTTCG                                                  >  SRR4302518.4137142/1‑101 (MQ=60)
                                                      TCTGTTTAAGCTGCTGGTGATAGCGGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCTCCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGG                                                 <  SRR4302518.4226980/101‑1 (MQ=60)
                                                      NNNGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCATCGTTGCCG                                                 <  SRR4302518.13598037/101‑1 (MQ=60)
                                                      NNNGTTTAAGCTGCTGGGGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGG                                                 <  SRR4302518.9986221/101‑1 (MQ=60)
                                                      NNNGTTAAAGCTGCTGGTGATAGCGGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGG                                                 <  SRR4302518.9511430/101‑1 (MQ=60)
                                                      GTTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGNNN                                                 >  SRR4302518.12203970/1‑101 (MQ=60)
                                                      GCTGTTTATGCTGGTGGTGATGGCGGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGG                                                 <  SRR4302518.5638205/101‑1 (MQ=60)
                                                       CTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGCCCGCCAGCGTTGCGGG                                                <  SRR4302518.5436813/101‑1 (MQ=60)
                                                       CTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGG                                                <  SRR4302518.313136/101‑1 (MQ=60)
                                                           nnnatcgGCTGGTGATAGCGGAAGAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGTT                                            <  SRR4302518.8725484/94‑1 (MQ=60)
                                                            taacatgtTGGTGATAGCGGAATAACGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATG                                           <  SRR4302518.5785684/93‑1 (MQ=60)
                                                            TAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATG                                           <  SRR4302518.4653830/101‑1 (MQ=60)
                                                            TAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATG                                           <  SRR4302518.523600/101‑1 (MQ=60)
                                                            NNNGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATG                                           <  SRR4302518.8787949/101‑1 (MQ=60)
                                                             nnncgGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGC                                          <  SRR4302518.11363150/96‑1 (MQ=60)
                                                             ACGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGC                                          <  SRR4302518.5323153/101‑1 (MQ=60)
                                                             AAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGANNN                                          >  SRR4302518.12183286/1‑101 (MQ=60)
                                                             AAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGANNN                                          >  SRR4302518.10510268/1‑101 (MQ=60)
                                                              AGCTGCTGGTGATAGCGGAAAAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCG                                         <  SRR4302518.7336301/101‑1 (MQ=60)
                                                                CTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGAG                                       >  SRR4302518.386375/1‑101 (MQ=60)
                                                                  NNNGGTGATAGCGAAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGG                                     <  SRR4302518.12583423/101‑1 (MQ=60)
                                                                  GCGGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGG                                     <  SRR4302518.777194/101‑1 (MQ=60)
                                                                   NNNGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA                                    <  SRR4302518.12397620/101‑1 (MQ=60)
                                                                   NNNGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA                                    <  SRR4302518.13997882/101‑1 (MQ=60)
                                                                   CTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CGAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATNNN                                    >  SRR4302518.13538358/1‑101 (MQ=60)
                                                                   CTGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCAACTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGA                                    <  SRR4302518.185718/101‑1 (MQ=60)
                                                                    TGGTGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGATCGCCAGCGATGTGGGCGATGCGATTGAT                                   >  SRR4302518.6460199/1‑101 (MQ=60)
                                                                      GGGATAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CGAAGCCACCATCTCCGGCACTAAAGCGGGAGGCATGACGGCCAGCGTTCCGGGCGATGCGGTGGANNN                                 >  SRR4302518.11292786/1‑101 (MQ=60)
                                                                        GATAGCGGAAAAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGA                               <  SRR4302518.1975572/101‑1 (MQ=60)
                                                                        GAAAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGA                               <  SRR4302518.4645903/101‑1 (MQ=60)
                                                                          aagggcaataagggagctgttcgcgG‑GG‑CAAAGCCAACATCTCCGGCATTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATT                             <  SRR4302518.2895695/76‑1 (MQ=60)
                                                                          TAGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATT                             >  SRR4302518.3555607/1‑101 (MQ=60)
                                                                          NNNCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATT                             <  SRR4302518.10793421/101‑1 (MQ=60)
                                                                           AGCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGTACTAAAGCTGGTCGCATGACCGCCAGCGTagcgatcctcggaaggcaattgattg                            >  SRR4302518.5697033/1‑75 (MQ=60)
                                                                           AGCGAAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTG                            <  SRR4302518.843598/101‑1 (MQ=60)
                                                                            NNNGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGT                           <  SRR4302518.8782775/101‑1 (MQ=60)
                                                                            GCGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATNNN                           >  SRR4302518.13628794/1‑101 (MQ=60)
                                                                             NNNAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCCATGCGATGGATTTGATTGTC                          <  SRR4302518.10037220/101‑1 (MQ=60)
                                                                             CGGAATAAGTGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTC                          <  SRR4302518.5144445/101‑1 (MQ=60)
                                                                             CGGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTC                          >  SRR4302518.6086931/1‑101 (MQ=60)
                                                                              GGAATAAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCC                         <  SRR4302518.2607147/101‑1 (MQ=60)
                                                                              GGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCC                         >  SRR4302518.769393/1‑101 (MQ=60)
                                                                              GGAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACAGCCAGCGGTGCGGGCGATGCGATGGGTTTGATTGNNN                         >  SRR4302518.8208653/1‑101 (MQ=60)
                                                                               nnncacAGGAGCCGATAGCGG‑GG‑CAAAGCCGCCATCTCCGGCACTCAAGCGGGTCGCATGACCGCCTGCGTTGCGGTCGATGCGATGGATTTGATTGTCTT                        <  SRR4302518.12322288/95‑1 (MQ=60)
                                                                               NNNTAAAGGAGCCGATAGCGG‑GG‑CAAAGACACCATCTCCGGCACTAAAGCGGGTCGCATGATCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTCTCCT                        <  SRR4302518.10639358/101‑1 (MQ=60)
                                                                               GAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCT                        <  SRR4302518.7269365/101‑1 (MQ=60)
                                                                               GAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCT                        <  SRR4302518.257204/101‑1 (MQ=60)
                                                                               GAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCT                        <  SRR4302518.4629952/101‑1 (MQ=60)
                                                                               GAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCT                        <  SRR4302518.6965706/101‑1 (MQ=60)
                                                                               GAATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCAACATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGGTGCGAGGGATTTtatggtnnn                        >  SRR4302518.14295495/1‑92 (MQ=60)
                                                                                AATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTC                       <  SRR4302518.1330727/101‑1 (MQ=60)
                                                                                AATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTC                       <  SRR4302518.6429150/101‑1 (MQ=60)
                                                                                AATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTC                       <  SRR4302518.5195922/101‑1 (MQ=60)
                                                                                 NNNAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCG                      <  SRR4302518.12804631/101‑1 (MQ=60)
                                                                                 ATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCG                      >  SRR4302518.5181649/1‑101 (MQ=60)
                                                                                 ATAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATACGATGGATTTGATTGTCCNNN                      >  SRR4302518.10816075/1‑101 (MQ=60)
                                                                                  TAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGG                     >  SRR4302518.4998795/1‑101 (MQ=60)
                                                                                  AAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGG                     <  SRR4302518.6983795/101‑1 (MQ=60)
                                                                                  AAAAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGG                     <  SRR4302518.6845248/101‑1 (MQ=60)
                                                                                   AAGGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGAGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGC                    <  SRR4302518.817443/101‑1 (MQ=60)
                                                                                    AAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCT                   >  SRR4302518.5654108/1‑101 (MQ=60)
                                                                                    AAGGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCT                   >  SRR4302518.46537/1‑101 (MQ=60)
                                                                                    AAGGAGCCGATAGCGG‑GG‑CAAAGCCACCAGCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGAGTGTCCTCGNNN                   >  SRR4302518.8685337/1‑101 (MQ=60)
                                                                                     AGGAGCCGATAGC‑GCGG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTG                  <  SRR4302518.3796897/101‑1 (MQ=60)
                                                                                     AAGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGNNN                  >  SRR4302518.14981488/1‑101 (MQ=60)
                                                                                      GGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGT                 >  SRR4302518.398504/1‑101 (MQ=60)
                                                                                      GGAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGT                 <  SRR4302518.1937003/101‑1 (MQ=60)
                                                                                       gcgccgatagggG‑GG‑CAAAGCCCCCCTCTCCGGCACTAAAGCGGGTCGCATGACCCCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTC                <  SRR4302518.4491273/89‑1 (MQ=60)
                                                                                       GAGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTC                <  SRR4302518.3045834/101‑1 (MQ=60)
                                                                                        AGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGNNN               >  SRR4302518.13850544/1‑101 (MQ=60)
                                                                                        AGCCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGNNN               >  SRR4302518.7621379/1‑101 (MQ=60)
                                                                                          CCGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGAT             >  SRR4302518.2718646/1‑101 (MQ=60)
                                                                                          CCGATAGCGG‑GG‑CAAAGCCACCATCTCCCGCACTAAAGCGGGGCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGGTTTGATTGTCCTCGGCTGTCNNN             >  SRR4302518.9468992/1‑101 (MQ=60)
                                                                                           caatagcgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGNNN            >  SRR4302518.11655671/9‑101 (MQ=60)
                                                                                           CGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGNNN            >  SRR4302518.7888928/1‑101 (MQ=60)
                                                                                           CGATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATT            <  SRR4302518.2943153/101‑1 (MQ=60)
                                                                                            nnnagcgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTT           <  SRR4302518.9271529/94‑1 (MQ=60)
                                                                                            GATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGGCGCATGACCGCCAGCGTTGCGGGGGATGCGAAGGATTTGATTGTCCTtggcgcacaatta           >  SRR4302518.1539730/1‑88 (MQ=60)
                                                                                             nnngcgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTC          <  SRR4302518.13522480/95‑1 (MQ=60)
                                                                                             nnngcgG‑GG‑CAAAGCCACCACCTCCGGCACTAAAGCGCGTCTCATGACCGACAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTC          <  SRR4302518.10320678/95‑1 (MQ=60)
                                                                                             ATAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCAAGACCGCCAGCGTTGCGGTCGATTCGATGGAATTTATTGTCCTAGGCTGTCGATGTC          >  SRR4302518.2070038/1‑101 (MQ=60)
                                                                                              TAGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCA         <  SRR4302518.5901493/101‑1 (MQ=60)
                                                                                               AGCGG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAG        >  SRR4302518.2918293/1‑101 (MQ=60)
                                                                                                gggG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGGCGATTTCNNN       >  SRR4302518.9786833/4‑101 (MQ=60)
                                                                                                gcgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCNNN       >  SRR4302518.13787163/4‑101 (MQ=60)
                                                                                                 cgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCANNN      >  SRR4302518.8995445/3‑101 (MQ=60)
                                                                                                 cgG‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCC      <  SRR4302518.4249830/99‑1 (MQ=60)
                                                                                                 G‑G‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGNNN     >  SRR4302518.14591726/1‑101 (MQ=60)
                                                                                                 G‑G‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTAAGCCG     >  SRR4302518.2093919/1‑101 (MQ=60)
                                                                                                   N‑NN‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAG    <  SRR4302518.8106476/101‑1 (MQ=60)
                                                                                                   G‑GG‑CAGAGCCGTCATCTCCGACACTAAAGCGGGCCGCATAGCCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGGTTTCAGCCAG    <  SRR4302518.6011349/101‑1 (MQ=60)
                                                                                                   G‑GG‑CAAAGCCACCATCTCCGGCACTACAGCGGCACGCACGACCGCCAGCGTTACGGGCGATGCGATGGATTTGATTactctcgggtgtcgatttcaaccac    >  SRR4302518.7014564/1‑76 (MQ=60)
                                                                                                     NN‑NAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGC   <  SRR4302518.14398960/101‑1 (MQ=60)
                                                                                                     GG‑CAAAGCCACCAGCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGGCGCTTTCAGCCNNN   >  SRR4302518.14060565/1‑101 (MQ=60)
                                                                                                      G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCANNN  >  SRR4302518.7577171/1‑101 (MQ=60)
                                                                                                      G‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCANNN  >  SRR4302518.11032325/1‑101 (MQ=60)
                                                                                                      |                                                                                                     
CTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGC‑G‑GG‑CAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCG  >  NC_000913/3123485‑3123685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑