Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,093,861	T→C	L8P (CTT→CCT)	yggT →	putative resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,093,861	0	T	C	99.6%	834.4 / NA	256	L8P (CTT→CCT)	yggT	putative resistance protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (133/122); total (133/123)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCAC  >  NC_000913/3093769‑3093950

tGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCAATAAATACGTTGATTTTCTTGCt                                                                                           >  1:2151940/1‑93 (MQ=255)

  gTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCctt                                                                                         >  1:3645596/1‑93 (MQ=255)

   ttCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTc                                                                                        >  1:3775772/1‑93 (MQ=255)

     cGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCaa                                                                                      <  1:5034961/93‑1 (MQ=255)

      gTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAAc                                                                                     >  2:2457820/1‑93 (MQ=255)

       tATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACg                                                                                    <  1:3391801/93‑1 (MQ=255)

        aTCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACgg                                                                                   >  2:4156318/1‑93 (MQ=255)

        aTCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACgg                                                                                   >  1:2648264/1‑93 (MQ=255)

         tCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGt                                                                                  >  2:3929790/1‑93 (MQ=255)

         tCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGt                                                                                  >  1:3036203/1‑93 (MQ=255)

           ggCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCa                                                                                <  2:5960729/93‑1 (MQ=255)

           ggCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCa                                                                                >  1:5917598/1‑93 (MQ=255)

           agCACTGCAATTTTTGGTGCGAGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCa                                                                                <  2:1968454/92‑1 (MQ=255)

            gCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCAt                                                                               <  1:3094654/93‑1 (MQ=255)

            gCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCAt                                                                               <  1:5001573/93‑1 (MQ=255)

             cACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCAtt                                                                              >  2:290857/1‑93 (MQ=255)

             cACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCAtt                                                                              >  2:2153590/1‑93 (MQ=255)

              aCTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTg                                                                             >  1:6155376/1‑93 (MQ=255)

              aCTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTg                                                                             <  1:2566105/93‑1 (MQ=255)

              aCTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTg                                                                             <  2:4816636/93‑1 (MQ=255)

               cTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGa                                                                            <  2:1197683/93‑1 (MQ=255)

               cTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGa                                                                            >  2:4975837/1‑93 (MQ=255)

               cTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGa                                                                            <  2:18783/93‑1 (MQ=255)

                tGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAg                                                                           >  2:6202998/1‑93 (MQ=255)

                 gCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGACATGAATACGTTGACTTTCCTGCCTTCTACGGTCATTGAGc                                                                          >  2:5686614/1‑93 (MQ=255)

                    aTTTTTGGAGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGt                                                                       >  2:6138193/1‑93 (MQ=255)

                     tttttGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGta                                                                      <  1:2106800/93‑1 (MQ=255)

                          ggTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCa                                                                 >  1:409379/1‑93 (MQ=255)

                           gTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAAC‑GTCATTGAGCTGTATACCATg                                                               <  2:73233/93‑1 (MQ=255)

                               gcgTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTg                                                            >  2:5150385/1‑93 (MQ=255)

                                cgTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGc                                                           <  2:318528/93‑1 (MQ=255)

                                 gTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCt                                                          >  2:1585624/1‑93 (MQ=255)

                                  tGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTg                                                         >  1:4919897/1‑93 (MQ=255)

                                  tGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTg                                                         >  1:2017002/1‑93 (MQ=255)

                                   gATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGt                                                        >  1:1080954/1‑93 (MQ=255)

                                    aTTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCGTGGTGCTGtt                                                       >  1:5624171/1‑93 (MQ=255)

                                    aTTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGtt                                                       >  1:6032767/1‑93 (MQ=255)

                                    aTTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGtt                                                       <  1:4534068/93‑1 (MQ=255)

                                     ttACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGtta                                                      <  1:1184802/93‑1 (MQ=255)

                                     ttACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGtta                                                      <  1:5353934/93‑1 (MQ=255)

                                      tACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttat                                                     >  1:620960/1‑93 (MQ=255)

                                        ctctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttatta                                                   >  2:2517724/1‑93 (MQ=255)

                                        ctctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGttatta                                                   <  2:3769838/93‑1 (MQ=255)

                                         tctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTAc                                                  >  1:2525181/1‑93 (MQ=255)

                                          ctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACg                                                 >  2:4718655/1‑93 (MQ=255)

                                          ctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACg                                                 >  2:819183/1‑93 (MQ=255)

                                          ctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACg                                                 >  1:857685/1‑93 (MQ=255)

                                          ctAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACg                                                 >  2:3966965/1‑93 (MQ=255)

                                           tAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGc                                                <  1:1323725/93‑1 (MQ=255)

                                           tAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGc                                                >  2:1515336/1‑93 (MQ=255)

                                              aaaaTAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATc                                             <  2:4196509/93‑1 (MQ=255)

                                               aaaTAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCt                                            <  2:4447929/93‑1 (MQ=255)

                                                aaTAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTg                                           <  1:2938414/93‑1 (MQ=255)

                                                 aTAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTgg                                          >  2:360051/1‑93 (MQ=255)

                                                   aaGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGAt                                        <  1:585556/93‑1 (MQ=255)

                                                   aaGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGAt                                        >  1:2479323/1‑93 (MQ=255)

                                                    aGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATg                                       <  2:2381060/93‑1 (MQ=255)

                                                     ggAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGc                                      >  1:921174/1‑93 (MQ=255)

                                                         ttAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTg                                  >  2:5077016/1‑93 (MQ=255)

                                                          tAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTgg                                 <  1:2902879/93‑1 (MQ=255)

                                                          tAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTgg                                 >  1:165950/1‑93 (MQ=255)

                                                            aaGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGc                               >  1:188517/1‑93 (MQ=255)

                                                             aGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCt                              >  1:4566734/1‑93 (MQ=255)

                                                             aGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCt                              >  2:2802040/1‑93 (MQ=255)

                                                              ggAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTc                             >  2:490018/1‑93 (MQ=255)

                                                              ggAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTc                             >  2:3648868/1‑93 (MQ=255)

                                                              ggAACACCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACAATGGCGCTGTTATTACGCATCTGGCTGCAGTGGGCTc                             >  2:1994381/1‑93 (MQ=255)

                                                               gAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCa                            >  1:2697462/1‑93 (MQ=255)

                                                                aaCGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCAt                           <  2:2367118/93‑1 (MQ=255)

                                                                aaCGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCAt                           <  1:2226868/93‑1 (MQ=255)

                                                                aaCGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCAt                           <  2:4247899/93‑1 (MQ=255)

                                                                aaCGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCAt                           >  1:425591/1‑93 (MQ=255)

                                                                 aCGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCGGTGGGCTCAtt                          <  2:121115/93‑1 (MQ=255)

                                                                   gCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGTTCATtgt                        >  2:1342735/1‑93 (MQ=255)

                                                                   gCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATtgt                        <  2:5612553/93‑1 (MQ=255)

                                                                   gCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATtgt                        >  1:1770392/1‑93 (MQ=255)

                                                                   gCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATtgt                        <  2:6279765/93‑1 (MQ=255)

                                                                     cATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGa                      <  1:1866510/93‑1 (MQ=255)

                                                                     cATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGa                      <  1:4373637/93‑1 (MQ=255)

                                                                     cATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGa                      <  2:5176584/93‑1 (MQ=255)

                                                                     cATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGa                      <  2:1117477/93‑1 (MQ=255)

                                                                      aTGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAt                     >  1:5499358/1‑93 (MQ=255)

                                                                       tGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAtt                    >  1:3870284/1‑93 (MQ=255)

                                                                       tGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAtt                    <  2:4749992/93‑1 (MQ=255)

                                                                          aTACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAttttt                 >  1:4626522/1‑93 (MQ=255)

                                                                          aTACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAttttt                 >  2:1821101/1‑93 (MQ=255)

                                                                           tACGTTGCCTTCCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTa                <  2:2977685/93‑1 (MQ=255)

                                                                           tACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGAATCTGGATGCAGTGGGCTCATTGTGATTTTTa                >  1:5410719/1‑93 (MQ=255)

                                                                             cGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACa              >  2:3311408/1‑93 (MQ=255)

                                                                              gTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACaa             <  2:4465698/93‑1 (MQ=255)

                                                                               ttGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATGCCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAc            <  1:5737443/93‑1 (MQ=255)

                                                                               ttGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAc            <  1:4118659/93‑1 (MQ=255)

                                                                               ttGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAc            <  2:5380812/93‑1 (MQ=255)

                                                                                tGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAcc           <  2:3378311/93‑1 (MQ=255)

                                                                                 gACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAccc          <  1:1035386/93‑1 (MQ=255)

                                                                                 gACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAccc          >  1:4327732/1‑93 (MQ=255)

                                                                                     ttCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTtc      >  1:703751/1‑93 (MQ=255)

                                                                                     ttCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTtc      <  1:6051723/93‑1 (MQ=255)

                                                                                      tCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTtct     >  1:1456549/1‑93 (MQ=255)

                                                                                         tGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTcac  >  1:4847583/1‑93 (MQ=255)

TGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCAC  >  NC_000913/3093769‑3093950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAGTT  >  NC_000913/3093768‑3093954

ATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCC                                                                                               <  SRR4302533.5485172/94‑1 (MQ=60)

ATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCAATAAATACGTTGATTTTCTTGCT                                                                                               >  SRR4302533.2165194/1‑94 (MQ=60)

 TGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCT                                                                                              <  SRR4302533.2157635/94‑1 (MQ=60)

  GGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTT                                                                                             >  SRR4302533.5896786/1‑94 (MQ=60)

  GGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTT                                                                                             >  SRR4302533.3668009/1‑94 (MQ=60)

   GTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTC                                                                                            >  SRR4302533.5179076/1‑94 (MQ=60)

   GTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTC                                                                                            <  SRR4302533.1322104/94‑1 (MQ=60)

   GTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTC                                                                                            >  SRR4302533.3798917/1‑94 (MQ=60)

    TTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCA                                                                                           <  SRR4302533.3012282/94‑1 (MQ=60)

      CGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAAC                                                                                         <  SRR4302533.5065457/94‑1 (MQ=60)

       GTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACG                                                                                        >  SRR4302533.6157698/1‑94 (MQ=60)

        TATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGTCTTTCCTGCCTTCAACGG                                                                                       <  SRR4302533.6028946/94‑1 (MQ=60)

        TATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGG                                                                                       <  SRR4302533.3412747/94‑1 (MQ=60)

        TATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGG                                                                                       >  SRR4302533.2664532/1‑94 (MQ=60)

        TATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGG                                                                                       >  SRR4302533.30965/1‑94 (MQ=60)

         ATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGT                                                                                      >  SRR4302533.3054781/1‑94 (MQ=60)

         ATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGT                                                                                      >  SRR4302533.2489482/1‑94 (MQ=60)

         ATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGT                                                                                      <  SRR4302533.1391781/94‑1 (MQ=60)

           CGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCA                                                                                    >  SRR4302533.1539611/1‑94 (MQ=60)

           CGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCA                                                                                    >  SRR4302533.5953360/1‑94 (MQ=60)

             GCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATT                                                                                  <  SRR4302533.3113679/94‑1 (MQ=60)

             GCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATT                                                                                  <  SRR4302533.5031892/94‑1 (MQ=60)

              CACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTG                                                                                 >  SRR4302533.6192408/1‑94 (MQ=60)

              CACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTG                                                                                 >  SRR4302533.2560696/1‑94 (MQ=60)

              CACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTG                                                                                 <  SRR4302533.4297248/94‑1 (MQ=60)

               ACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGA                                                                                <  SRR4302533.2581896/94‑1 (MQ=60)

                CTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAG                                                                               >  SRR4302533.5910432/1‑94 (MQ=60)

                CTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAG                                                                               <  SRR4302533.3360751/94‑1 (MQ=60)

                CTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAG                                                                               >  SRR4302533.4361665/1‑94 (MQ=60)

                 TGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGC                                                                              <  SRR4302533.1060672/94‑1 (MQ=60)

                    AATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGT                                                                           <  SRR4302533.501219/94‑1 (MQ=60)

                      TTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTAT                                                                         <  SRR4302533.3760617/94‑1 (MQ=60)

                      TTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTAT                                                                         <  SRR4302533.2119790/94‑1 (MQ=60)

                        TTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATAC                                                                       >  SRR4302533.4061803/1‑94 (MQ=60)

                        TTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATAC                                                                       <  SRR4302533.2068863/94‑1 (MQ=60)

                          TGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCA                                                                     >  SRR4302533.411835/1‑94 (MQ=60)

                          TGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCA                                                                     <  SRR4302533.252265/94‑1 (MQ=60)

                              GCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGT                                                                 <  SRR4302533.5192942/94‑1 (MQ=60)

                                 CGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCT                                                              >  SRR4302533.2823761/1‑94 (MQ=60)

                                 CGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCT                                                              >  SRR4302533.2208539/1‑94 (MQ=60)

                                 CGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCT                                                              <  SRR4302533.2001076/94‑1 (MQ=60)

                                  GTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTG                                                             >  SRR4302533.4949700/1‑94 (MQ=60)

                                  GTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTG                                                             >  SRR4302533.5515733/1‑94 (MQ=60)

                                  GTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTG                                                             >  SRR4302533.2029429/1‑94 (MQ=60)

                                  GTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTG                                                             >  SRR4302533.4769123/1‑94 (MQ=60)

                                  GTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTG                                                             <  SRR4302533.3542032/94‑1 (MQ=60)

                                   TGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGT                                                            >  SRR4302533.722759/1‑94 (MQ=60)

                                   TGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGT                                                            >  SRR4302533.2773069/1‑94 (MQ=60)

                                   TGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGT                                                            >  SRR4302533.4506685/1‑94 (MQ=60)

                                   TGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGT                                                            >  SRR4302533.1087331/1‑94 (MQ=60)

                                    GATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCGTGGTGCTGTT                                                           >  SRR4302533.5658165/1‑94 (MQ=60)

                                    GATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTT                                                           >  SRR4302533.6069328/1‑94 (MQ=60)

                                     ATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTA                                                          <  SRR4302533.4561522/94‑1 (MQ=60)

                                      TTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTAT                                                         <  SRR4302533.5386069/94‑1 (MQ=60)

                                      TTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTAT                                                         <  SRR4302533.1191914/94‑1 (MQ=60)

                                      TTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTAT                                                         >  SRR4302533.624609/1‑94 (MQ=60)

                                        ACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTA                                                       <  SRR4302533.4355879/94‑1 (MQ=60)

                                        ACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTA                                                       >  SRR4302533.774251/1‑94 (MQ=60)

                                        ACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTA                                                       >  SRR4302533.1579794/1‑94 (MQ=60)

                                         CTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTAC                                                      >  SRR4302533.5638170/1‑94 (MQ=60)

                                         CTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTAC                                                      >  SRR4302533.2540731/1‑94 (MQ=60)

                                          TCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACG                                                     >  SRR4302533.862707/1‑94 (MQ=60)

                                            TAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCA                                                   <  SRR4302533.1331663/94‑1 (MQ=60)

                                                 AATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAATGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGG                                              <  SRR4302533.4470344/94‑1 (MQ=60)

                                                 AATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGG                                              <  SRR4302533.2223713/94‑1 (MQ=60)

                                                 AATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGG                                              <  SRR4302533.2956369/94‑1 (MQ=60)

                                                 AATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTCCAACGGTCATTGAGCTGAATACCATGGTGCTGTTATTACGCATCTGG                                              >  SRR4302533.1830194/1‑94 (MQ=60)

                                                   TAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGAT                                            >  SRR4302533.2494613/1‑94 (MQ=60)

                                                   TAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGAT                                            <  SRR4302533.4139490/94‑1 (MQ=60)

                                                   AAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGAT                                            <  SRR4302533.2283118/94‑1 (MQ=60)

                                                    AAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATG                                           <  SRR4302533.2693145/94‑1 (MQ=60)

                                                    AAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATG                                           <  SRR4302533.589008/94‑1 (MQ=60)

                                                    AAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATG                                           <  SRR4302533.1894538/94‑1 (MQ=60)

                                                     AGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGC                                          >  SRR4302533.926582/1‑94 (MQ=60)

                                                     AGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGC                                          >  SRR4302533.619491/1‑94 (MQ=60)

                                                     AGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGC                                          >  SRR4302533.4231820/1‑94 (MQ=60)

                                                      GGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCA                                         >  SRR4302533.3885011/1‑94 (MQ=60)

                                                       GAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAG                                        <  SRR4302533.562448/94‑1 (MQ=60)

                                                        AATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGT                                       <  SRR4302533.876461/94‑1 (MQ=60)

                                                         ATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTG                                      <  SRR4302533.5014530/94‑1 (MQ=60)

                                                         ATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTG                                      >  SRR4302533.2697259/1‑94 (MQ=60)

                                                         ATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTG                                      <  SRR4302533.1869175/94‑1 (MQ=60)

                                                          TTAATGGAACGCCATGAATACGTTGACTTTCCTGCCTCCAACGGTCATTGAGCTGTATGCCGTGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     <  SRR4302533.2762182/94‑1 (MQ=60)

                                                          TTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     >  SRR4302533.5699580/1‑94 (MQ=60)

                                                          TTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     >  SRR4302533.1728883/1‑94 (MQ=60)

                                                          TTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     >  SRR4302533.167003/1‑94 (MQ=60)

                                                          TTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     <  SRR4302533.505656/94‑1 (MQ=60)

                                                          TTAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGG                                     <  SRR4302533.3033564/94‑1 (MQ=60)

                                                           TAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGG                                    <  SRR4302533.2920709/94‑1 (MQ=60)

                                                           TAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGG                                    <  SRR4302533.4608658/94‑1 (MQ=60)

                                                           TAAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGG                                    <  SRR4302533.3063545/94‑1 (MQ=60)

                                                            AAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGC                                   >  SRR4302533.189690/1‑94 (MQ=60)

                                                            AAAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGC                                   >  SRR4302533.5394485/1‑94 (MQ=60)

                                                             AAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCT                                  >  SRR4302533.4594420/1‑94 (MQ=60)

                                                             AAGGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCT                                  <  SRR4302533.4742213/94‑1 (MQ=60)

                                                               GGAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCA                                >  SRR4302533.2713989/1‑94 (MQ=60)

                                                                GAACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCAT                               >  SRR4302533.428129/1‑94 (MQ=60)

                                                                 AACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATT                              <  SRR4302533.2240615/94‑1 (MQ=60)

                                                                  ACGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTG                             <  SRR4302533.956331/94‑1 (MQ=60)

                                                                   CGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGT                            >  SRR4302533.1781172/1‑94 (MQ=60)

                                                                   CGCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGT                            >  SRR4302533.2111602/1‑94 (MQ=60)

                                                                    GCCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTG                           >  SRR4302533.4599530/1‑94 (MQ=60)

                                                                     CCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGA                          <  SRR4302533.4572724/94‑1 (MQ=60)

                                                                     CCATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGA                          >  SRR4302533.4051602/1‑94 (MQ=60)

                                                                      CATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAT                         <  SRR4302533.1877894/94‑1 (MQ=60)

                                                                      CATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAT                         >  SRR4302533.3880633/1‑94 (MQ=60)

                                                                      CATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAT                         <  SRR4302533.4400037/94‑1 (MQ=60)

                                                                      CATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGAT                         >  SRR4302533.5532531/1‑94 (MQ=60)

                                                                       ATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATT                        <  SRR4302533.112638/94‑1 (MQ=60)

                                                                       ATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATT                        >  SRR4302533.3893909/1‑94 (MQ=60)

                                                                       ATGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGCCTCATTGTGATT                        >  SRR4302533.544276/1‑94 (MQ=60)

                                                                        TGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTT                       >  SRR4302533.626295/1‑94 (MQ=60)

                                                                        TGAATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTT                       <  SRR4302533.4885324/94‑1 (MQ=60)

                                                                          AATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTT                     >  SRR4302533.6078626/1‑94 (MQ=60)

                                                                          AATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTT                     >  SRR4302533.4654561/1‑94 (MQ=60)

                                                                           ATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTA                    >  SRR4302533.6304500/1‑94 (MQ=60)

                                                                           ATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTA                    >  SRR4302533.5778863/1‑94 (MQ=60)

                                                                           ATACGTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGAATCTGGATGCAGTGGGCTCATTGTGATTTTTA                    >  SRR4302533.5443250/1‑94 (MQ=60)

                                                                               GTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAC                <  SRR4302533.3558063/94‑1 (MQ=60)

                                                                               GTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAC                <  SRR4302533.1383384/94‑1 (MQ=60)

                                                                               GTTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAAC                >  SRR4302533.1963127/1‑94 (MQ=60)

                                                                                TTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATGCCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACC               <  SRR4302533.5772072/94‑1 (MQ=60)

                                                                                TTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACC               <  SRR4302533.5716290/94‑1 (MQ=60)

                                                                                TTGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACC               <  SRR4302533.4143674/94‑1 (MQ=60)

                                                                                 TGACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCC              >  SRR4302533.4353801/1‑94 (MQ=60)

                                                                                  GACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCC             <  SRR4302533.1041474/94‑1 (MQ=60)

                                                                                  GACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCC             <  SRR4302533.2140591/94‑1 (MQ=60)

                                                                                   ACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCT            >  SRR4302533.6084798/1‑94 (MQ=60)

                                                                                   ACTTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCT            <  SRR4302533.2662649/94‑1 (MQ=60)

                                                                                     TTTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTC          >  SRR4302533.707911/1‑94 (MQ=60)

                                                                                      TTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCT         <  SRR4302533.6088380/94‑1 (MQ=60)

                                                                                      TTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCT         <  SRR4302533.2541094/94‑1 (MQ=60)

                                                                                      TTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCT         >  SRR4302533.1465330/1‑94 (MQ=60)

                                                                                      TTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCT         <  SRR4302533.1182894/94‑1 (MQ=60)

                                                                                      TTCCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCT         <  SRR4302533.4210955/94‑1 (MQ=60)

                                                                                        CCTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCA       >  SRR4302533.6088401/1‑94 (MQ=60)

                                                                                         CTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCAC      >  SRR4302533.2928251/1‑94 (MQ=60)

                                                                                         CTGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCAC      >  SRR4302533.4876961/1‑94 (MQ=60)

                                                                                          TGCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACA     >  SRR4302533.4078301/1‑94 (MQ=60)

                                                                                           GCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAG    >  SRR4302533.617081/1‑94 (MQ=60)

                                                                                           GCCTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAG    >  SRR4302533.2771079/1‑94 (MQ=60)

                                                                                             CTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAGTT  >  SRR4302533.2946006/1‑94 (MQ=60)

                                                                                             CTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAGTT  <  SRR4302533.3679295/94‑1 (MQ=60)

ATGGTTCGTATCGGCACTGCAATTTTTGGTGCGCGTGATTACTCTAAAAAATAAGGAATTAAAGGAACGCCATGAATACGTTGACTTTCCTGCTTTCAACGGTCATTGAGCTGTATACCATGGTGCTGTTATTACGCATCTGGATGCAGTGGGCTCATTGTGATTTTTACAACCCCTTCTCACAGTT  >  NC_000913/3093768‑3093954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`