Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,107,469 +C intergenic (‑296/‑101) b2965 ← / → yqgA ornithine decarboxylase isozyme/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,4691.C99.1% 681.4 / ‑1.4 215intergenic (‑296/‑101)b2965/yqgAornithine decarboxylase isozyme/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (77/136);  total (77/138)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 234 total aligned reads displayed.

GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555
                                                                                               |                                                                                      
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑t                                                                                         <  2:1115070/93‑1 (MQ=255)
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑t                                                                                         <  1:6177683/93‑1 (MQ=255)
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑t                                                                                         <  1:1628180/93‑1 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑tc                                                                                        <  2:4718957/93‑2 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑tc                                                                                        >  1:5559948/1‑92 (MQ=255)
  aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑tcg                                                                                       >  2:5846415/1‑91 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGGGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCt‑t‑tcgc                                                                                      >  1:1205285/1‑90 (MQ=255)
      gCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTaa                                                                                   <  1:1078459/93‑1 (MQ=255)
      gCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTaa                                                                                   >  2:1315096/1‑93 (MQ=255)
      gCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTaa                                                                                   <  1:4748682/93‑1 (MQ=255)
       cGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTaaa                                                                                  <  1:6119406/93‑1 (MQ=255)
       cGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTaaa                                                                                  <  2:4406939/93‑1 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAg                                                                                 <  1:5880829/93‑1 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAg                                                                                 >  1:5301049/1‑93 (MQ=255)
            aGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTg                                                                             <  1:1311930/93‑1 (MQ=255)
            aGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTg                                                                             <  2:4854318/93‑1 (MQ=255)
             gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGc                                                                            >  1:5249812/1‑93 (MQ=255)
             gCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGc                                                                            <  2:5908417/93‑1 (MQ=255)
               gagagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCaa                                                                          >  2:149781/1‑93 (MQ=255)
                 gagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGc                                                                        <  2:3312796/93‑1 (MQ=255)
                  agaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGcc                                                                       <  1:3784719/93‑1 (MQ=255)
                   gaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCa                                                                      <  2:1086959/93‑1 (MQ=255)
                   gaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCa                                                                      <  2:4586184/93‑1 (MQ=255)
                    aaTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAg                                                                     <  2:1417399/93‑1 (MQ=255)
                      tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGa                                                                   <  2:3950125/93‑1 (MQ=255)
                       gTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGAc                                                                  >  2:5505315/1‑93 (MQ=255)
                         aaGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACtt                                                                <  1:100954/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACttt                                                               <  1:4347915/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACttt                                                               >  2:1029633/1‑93 (MQ=255)
                               aTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaa                                                          <  1:3456301/93‑1 (MQ=255)
                                 ggCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                        <  2:30662/93‑1 (MQ=255)
                                 ggCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                        >  1:179464/1‑93 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                     <  1:1861256/93‑1 (MQ=255)
                                    gTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaac                                                     >  2:3052300/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCTCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    <  1:3835457/93‑1 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    >  1:1484464/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    >  1:3566331/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    <  1:2173700/93‑1 (MQ=255)
                                      gCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAacaaacaa                                                   <  2:2710226/93‑1 (MQ=255)
                                       cATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAg                                                  <  2:2383069/93‑1 (MQ=255)
                                       cATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAg                                                  <  2:1676859/93‑1 (MQ=255)
                                        aTCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGc                                                 >  2:3916100/1‑93 (MQ=255)
                                         tCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                >  1:1713248/1‑93 (MQ=255)
                                         agtggCAACCCCCGTACAGAGAGGAGAAAATTCGCGAAATCAGGGCCCCT‑T‑TCGCAAAGGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                <  2:1861368/90‑1 (MQ=255)
                                          cGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCa                                               <  2:1275273/93‑1 (MQ=255)
                                          cGGGAAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCa                                               <  1:78057/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              >  1:5147549/1‑93 (MQ=255)
                                             gCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCg                                            <  2:2443627/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  1:1477145/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          >  1:2520683/1‑93 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  2:5465201/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  1:4952305/93‑1 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         <  1:592227/93‑1 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         >  2:2579403/1‑93 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         <  1:340552/93‑1 (MQ=255)
                                                 aCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACgg                                        <  2:3127520/93‑1 (MQ=255)
                                                  cTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGc                                       <  1:2147240/93‑1 (MQ=255)
                                                   tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCa                                      >  1:3045182/1‑93 (MQ=255)
                                                      gTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCa                                   <  2:2227842/93‑1 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 >  1:4676776/1‑93 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 >  1:5434328/1‑93 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  1:5505315/93‑1 (MQ=255)
                                                          agagagGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACg                               <  1:4974004/93‑1 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              >  2:1268805/1‑93 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              <  2:2764914/93‑1 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              >  1:1092237/1‑93 (MQ=255)
                                                            agagGAGAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGt                             >  2:1771887/1‑93 (MQ=255)
                                                             gaggagAAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGta                            >  1:4857497/1‑92 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCCCCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         <  1:4280450/93‑1 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         >  1:417599/1‑93 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         <  1:397451/93‑1 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         >  1:804823/1‑93 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         <  1:1266842/93‑1 (MQ=255)
                                                                 agaAAATACGCGCAATCAAGGCTCCTGT‑C‑GCTAAAGCGTGCAAGCCAGGACTTGGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataa                        <  2:3852709/93‑1 (MQ=255)
                                                                   aaaaTTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataata                      >  2:5339166/1‑93 (MQ=255)
                                                                   aaaaTTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataata                      <  2:610257/93‑1 (MQ=255)
                                                                   aaaaTTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataata                      <  2:5133983/93‑1 (MQ=255)
                                                                    aaaTTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAtaataa                     <  1:211889/93‑1 (MQ=255)
                                                                     aaTTCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAg                    <  1:3695765/93‑1 (MQ=255)
                                                                      atgcgcgCAATCATGGCACCT‑TCC‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGa                   <  2:4618794/90‑1 (MQ=255)
                                                                        tCGCGCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaaa                 <  2:1735082/93‑1 (MQ=255)
                                                                         cgcgCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaaaa                <  2:2099045/93‑1 (MQ=255)
                                                                         cgcgCAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaaaa                <  2:4451652/93‑1 (MQ=255)
                                                                          gcgcAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt               <  1:2844791/93‑1 (MQ=255)
                                                                          gcgcAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt               >  1:6158056/1‑93 (MQ=255)
                                                                            gcAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCa             >  1:3209048/1‑93 (MQ=255)
                                                                             cAATCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaa            >  2:682423/1‑93 (MQ=255)
                                                                              aaTCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaaa           <  2:3195431/93‑1 (MQ=255)
                                                                                tCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         <  2:5126825/93‑1 (MQ=255)
                                                                                tCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         <  2:2027493/93‑1 (MQ=255)
                                                                                tCATGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         <  1:3721705/93‑1 (MQ=255)
                                                                                   tGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa      >  2:4302557/1‑93 (MQ=255)
                                                                                   tGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa      <  1:656346/93‑1 (MQ=255)
                                                                                   tGGCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATa      <  1:225858/93‑1 (MQ=255)
                                                                                    ggCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAc     <  1:5684653/93‑1 (MQ=255)
                                                                                    ggCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAc     >  1:2928364/1‑93 (MQ=255)
                                                                                    gccACCT‑T‑TCGTTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAc     <  2:5372396/91‑1 (MQ=255)
                                                                                     gCACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  2:33997/93‑1 (MQ=255)
                                                                                      cACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAg   >  2:1481623/1‑93 (MQ=255)
                                                                                      cACCT‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAg   <  1:364242/93‑1 (MQ=255)
                                                                                               |                                                                                      
GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCT‑T‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562
                                                                                             |                                                                                              
CGACACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT                                                                                                <  SRR4302533.6094666/94‑1 (MQ=60)
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT                                                                                                <  SRR4302533.2366209/94‑1 (MQ=60)
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT                                                                                                <  SRR4302533.4458896/94‑1 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑C                                                                                              <  SRR4302533.6214794/94‑1 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑C                                                                                              >  SRR4302533.3544608/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑C                                                                                              >  SRR4302533.5593551/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑C                                                                                              <  SRR4302533.1638040/94‑1 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑C                                                                                              >  SRR4302533.1492131/1‑94 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTcg                                                                                              <  SRR4302533.2820831/94‑3 (MQ=60)
   AACGGCGATAAGCGAGAGAATGTAAGGAGATGGGGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTcgc                                                                                             >  SRR4302533.1212528/1‑91 (MQ=60)
      GGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAA                                                                                          >  SRR4302533.6181277/1‑94 (MQ=60)
       GCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAA                                                                                         <  SRR4302533.1084814/94‑1 (MQ=60)
       GCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAA                                                                                         <  SRR4302533.4777474/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAG                                                                                        <  SRR4302533.3728274/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAG                                                                                        <  SRR4302533.6156310/94‑1 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAG                                                                                        >  SRR4302533.3624084/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAG                                                                                        >  SRR4302533.5332807/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAG                                                                                        >  SRR4302533.5240232/1‑94 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGC                                                                                       <  SRR4302533.377034/94‑1 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGC                                                                                       <  SRR4302533.5916366/94‑1 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGC                                                                                   >  SRR4302533.5281363/1‑94 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGC                                                                                   <  SRR4302533.2953172/94‑1 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGC                                                                                   <  SRR4302533.1319803/94‑1 (MQ=60)
              GCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCA                                                                                  <  SRR4302533.4513541/94‑1 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAG                                                                                <  SRR4302533.6341245/94‑1 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAG                                                                                <  SRR4302533.307967/94‑1 (MQ=60)
                  GAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCC                                                                              <  SRR4302533.4186349/94‑1 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCGAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCA                                                                             >  SRR4302533.1121178/1‑94 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCA                                                                             <  SRR4302533.1738235/94‑1 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCA                                                                             >  SRR4302533.3244989/1‑94 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCA                                                                             <  SRR4302533.3807893/94‑1 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCA                                                                             >  SRR4302533.1602309/1‑94 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302533.5899754/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302533.5996252/94‑1 (MQ=60)
                      ATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGggcatgcacgga                                                                          >  SRR4302533.1180219/1‑82 (MQ=60)
                      ATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGA                                                                          >  SRR4302533.5291596/1‑94 (MQ=60)
                      ATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGA                                                                          >  SRR4302533.1978911/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGAC                                                                         >  SRR4302533.497807/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGAC                                                                         <  SRR4302533.1944553/94‑1 (MQ=60)
                         TAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTT                                                                       <  SRR4302533.674785/94‑1 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302533.101581/94‑1 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302533.6188101/94‑1 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302533.915437/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     <  SRR4302533.5064943/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     <  SRR4302533.4374127/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     <  SRR4302533.2936011/94‑1 (MQ=60)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    >  SRR4302533.4536757/1‑94 (MQ=60)
                             GAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCG                                                                   <  SRR4302533.4033692/94‑1 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                <  SRR4302533.1672294/94‑1 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                <  SRR4302533.1146800/94‑1 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                >  SRR4302533.426018/1‑94 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                <  SRR4302533.3477634/94‑1 (MQ=60)
                                 TGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACA                                                               >  SRR4302533.180587/1‑94 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           >  SRR4302533.1493423/1‑94 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           <  SRR4302533.1872604/94‑1 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           >  SRR4302533.3588309/1‑94 (MQ=60)
                                      TGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCTCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAA                                                          <  SRR4302533.3858894/94‑1 (MQ=60)
                                      TGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAA                                                          <  SRR4302533.2187094/94‑1 (MQ=60)
                                      TGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAA                                                          <  SRR4302533.3671040/94‑1 (MQ=60)
                                       GCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAG                                                         <  SRR4302533.4367715/94‑1 (MQ=60)
                                        CATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACACACAAGC                                                        >  SRR4302533.2904989/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       <  SRR4302533.5707967/94‑1 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       >  SRR4302533.1723630/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       <  SRR4302533.2200714/94‑1 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       <  SRR4302533.3813054/94‑1 (MQ=60)
                                           CGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAT                                                     >  SRR4302533.5178653/1‑94 (MQ=60)
                                           CGGGAAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAT                                                     <  SRR4302533.78559/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCGCCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302533.2244904/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    >  SRR4302533.1470946/1‑94 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 >  SRR4302533.2536214/1‑94 (MQ=60)
                                               CAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAC                                                 <  SRR4302533.130128/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302533.4982298/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302533.4982297/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302533.1486072/94‑1 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302533.6197619/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               <  SRR4302533.595725/94‑1 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               <  SRR4302533.342631/94‑1 (MQ=60)
                                                  ACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGC                                              >  SRR4302533.5916546/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             <  SRR4302533.2160464/94‑1 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302533.4744154/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302533.3063823/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             <  SRR4302533.1840185/94‑1 (MQ=60)
                                                     CCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACC                                           >  SRR4302533.2183389/1‑94 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAA                                         <  SRR4302533.6243895/94‑1 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAA                                         >  SRR4302533.5593180/1‑94 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGACATCGACGGCACCAA                                         >  SRR4302533.5510619/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302533.4705120/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302533.5467054/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       >  SRR4302533.1085955/1‑94 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302533.5538534/94‑1 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     >  SRR4302533.1098697/1‑94 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     <  SRR4302533.5004163/94‑1 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   <  SRR4302533.796274/94‑1 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTA                                   >  SRR4302533.4886936/1‑94 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                <  SRR4302533.3591871/94‑1 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                >  SRR4302533.420104/1‑94 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                >  SRR4302533.809551/1‑94 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCCCCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302533.4306184/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               >  SRR4302533.1083890/1‑94 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302533.1274421/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302533.399854/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302533.3470678/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               >  SRR4302533.3928060/1‑94 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302533.5238255/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302533.213225/94‑1 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGA                          >  SRR4302533.4787875/1‑94 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGA                          <  SRR4302533.3718467/94‑1 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACCAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         >  SRR4302533.926693/1‑94 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         <  SRR4302533.5371950/94‑1 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         >  SRR4302533.5743789/1‑94 (MQ=60)
                                                                          CGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      <  SRR4302533.1694276/94‑1 (MQ=60)
                                                                          CGCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAT                      >  SRR4302533.6195096/1‑94 (MQ=60)
                                                                           GCGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATC                     <  SRR4302533.2862296/94‑1 (MQ=60)
                                                                            CGCAATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCA                    >  SRR4302533.3228813/1‑94 (MQ=60)
                                                                                ATCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                >  SRR4302533.5525354/1‑94 (MQ=60)
                                                                                 TCATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302533.3744545/94‑1 (MQ=60)
                                                                                  CATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGTGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAT              <  SRR4302533.4956388/94‑1 (MQ=60)
                                                                                  CATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAT              >  SRR4302533.2133482/1‑94 (MQ=60)
                                                                                   ATGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATA             <  SRR4302533.1222750/94‑1 (MQ=60)
                                                                                    TGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302533.660219/94‑1 (MQ=60)
                                                                                    TGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            >  SRR4302533.2946277/1‑94 (MQ=60)
                                                                                    TGGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302533.227265/94‑1 (MQ=60)
                                                                                     GGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           <  SRR4302533.229602/94‑1 (MQ=60)
                                                                                     GGCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           <  SRR4302533.5718989/94‑1 (MQ=60)
                                                                                      GCACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          >  SRR4302533.379113/1‑94 (MQ=60)
                                                                                       CACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         <  SRR4302533.3390966/94‑1 (MQ=60)
                                                                                       CACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         <  SRR4302533.366451/94‑1 (MQ=60)
                                                                                        ACCTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        <  SRR4302533.1363086/94‑1 (MQ=60)
                                                                                          CTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA      >  SRR4302533.1857238/1‑94 (MQ=60)
                                                                                          CTTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA      <  SRR4302533.3139144/94‑1 (MQ=60)
                                                                                           TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     <  SRR4302533.2306951/94‑1 (MQ=60)
                                                                                           TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     >  SRR4302533.620014/1‑94 (MQ=60)
                                                                                           TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     <  SRR4302533.4529078/94‑1 (MQ=60)
                                                                                           TTTCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     >  SRR4302533.3030659/1‑94 (MQ=60)
                                                                                           TTC‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACA    <  SRR4302533.2514594/94‑1 (MQ=60)
                                                                                             C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302533.6234830/1‑94 (MQ=60)
                                                                                             C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302533.4426600/1‑94 (MQ=60)
                                                                                             C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  <  SRR4302533.2995118/94‑1 (MQ=60)
                                                                                             |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTTT‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑