Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,112,040 T→C A110A (GCA→GCG)  b2972 ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,112,0400TC100.0% 755.8 / NA 238A110A (GCA→GCG) b2972putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (117/121);  total (117/121)

Only 100 of 253 total aligned reads displayed.

CTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAG  >  NC_000913/3111951‑3112129
                                                                                         |                                                                                         
cTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCt                                                                                        >  2:2453260/1‑93 (MQ=255)
 tCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTg                                                                                       >  2:1141036/1‑93 (MQ=255)
  cgcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGt                                                                                      <  1:4866943/93‑1 (MQ=255)
   gcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTc                                                                                     >  1:4604272/1‑93 (MQ=255)
   gcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTc                                                                                     >  2:2939376/1‑93 (MQ=255)
      gCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAAt                                                                                  <  2:5548873/93‑1 (MQ=255)
      gCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAAt                                                                                  >  1:2324155/1‑93 (MQ=255)
       cAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATa                                                                                 >  2:753593/1‑93 (MQ=255)
       cAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATa                                                                                 <  2:398368/93‑1 (MQ=255)
        aaTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATaa                                                                                >  1:5016864/1‑93 (MQ=255)
        aaTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATaa                                                                                <  2:4410857/93‑1 (MQ=255)
         aTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAACAAGCAAGCGCTGTCAATAAc                                                                               >  2:3017425/1‑93 (MQ=255)
         aTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAc                                                                               >  1:1120436/1‑93 (MQ=255)
         aTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAc                                                                               >  1:3822322/1‑93 (MQ=255)
          acAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACt                                                                              <  1:6076570/92‑1 (MQ=255)
           cAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTc                                                                             <  1:5673921/93‑1 (MQ=255)
            aGCCAGGCGGATAATATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTcc                                                                            <  2:4614168/93‑1 (MQ=255)
             gCCAGGCGGATAATATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTcca                                                                           <  1:3880/93‑1 (MQ=255)
               cAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTccacc                                                                         <  1:5471164/93‑1 (MQ=255)
               cAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTccacc                                                                         >  1:1625473/1‑93 (MQ=255)
                aGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTccacca                                                                        <  2:1640356/93‑1 (MQ=255)
                aGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTccacca                                                                        >  1:347609/1‑93 (MQ=255)
                aGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAACGCTGTCAATAACTccacca                                                                        >  2:2079543/1‑93 (MQ=255)
                 ggCGAATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAg                                                                       <  2:3651948/93‑1 (MQ=255)
                  gCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGc                                                                      <  2:2876734/93‑1 (MQ=255)
                     gaaaataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGa                                                                   <  1:4772072/90‑1 (MQ=255)
                     gATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGa                                                                   >  2:2720683/1‑93 (MQ=255)
                      ataataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGAt                                                                  <  1:3676746/93‑1 (MQ=255)
                       taataTCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                 <  2:6301656/93‑1 (MQ=255)
                       taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                 >  2:1395512/1‑93 (MQ=255)
                       taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                 <  1:89581/93‑1 (MQ=255)
                       taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                 >  2:6229429/1‑93 (MQ=255)
                         atatCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAc                                                               <  2:2463777/93‑1 (MQ=255)
                            tcatcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCt                                                            >  2:5799218/1‑93 (MQ=255)
                             catcaCCGCCTGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCtt                                                           <  1:676796/93‑1 (MQ=255)
                              atcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTg                                                          >  2:844411/1‑93 (MQ=255)
                               tcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGc                                                         <  1:4940966/93‑1 (MQ=255)
                               tcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGc                                                         >  2:740089/1‑93 (MQ=255)
                                 cccGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTg                                                       <  1:2664450/92‑1 (MQ=255)
                                   cGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAt                                                     <  1:1884567/93‑1 (MQ=255)
                                   cGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAt                                                     <  2:1387643/93‑1 (MQ=255)
                                     ccAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAttt                                                   <  2:5951500/93‑1 (MQ=255)
                                      cAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtttt                                                  <  2:4267035/93‑1 (MQ=255)
                                           cccATCCACTTTCTGGCCAGACCAGCCTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCt                                             <  2:4325870/93‑1 (MQ=255)
                                           cccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCt                                             <  1:2173043/93‑1 (MQ=255)
                                            ccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTg                                            >  2:481877/1‑93 (MQ=255)
                                            ccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTg                                            >  2:5369759/1‑93 (MQ=255)
                                             cATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGa                                           >  2:5792636/1‑93 (MQ=255)
                                             cATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGAAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGa                                           >  1:6005248/1‑93 (MQ=255)
                                              aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                          <  2:5588924/93‑1 (MQ=255)
                                              aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                          >  2:3127243/1‑93 (MQ=255)
                                              aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                          <  1:2618537/93‑1 (MQ=255)
                                               tCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACa                                         >  2:1220621/1‑93 (MQ=255)
                                                 cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                       >  1:197154/1‑93 (MQ=255)
                                                 cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                       <  1:6144044/93‑1 (MQ=255)
                                                   cTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcg                                     >  2:1187894/1‑93 (MQ=255)
                                                    tttCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcgc                                    <  1:5757271/93‑1 (MQ=255)
                                                    tttCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcgc                                    <  2:3356834/93‑1 (MQ=255)
                                                      tCTGGCCAGACCAGACTCGCTAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCgg                                  <  1:4206040/93‑1 (MQ=255)
                                                       cTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGc                                 <  1:1241891/93‑1 (MQ=255)
                                                          gCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgc                              <  1:1679836/93‑1 (MQ=255)
                                                           ccagaccagaCTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcg                             >  1:3580901/1‑93 (MQ=255)
                                                            cagaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcgc                            >  1:144173/1‑93 (MQ=255)
                                                            cagaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcgc                            <  2:5534394/93‑1 (MQ=255)
                                                            cagaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcgc                            >  2:6167845/1‑93 (MQ=255)
                                                            cagaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcgc                            >  1:2458099/1‑93 (MQ=255)
                                                             agaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGcc                           >  2:4484492/1‑93 (MQ=255)
                                                             agaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGAGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAccgac                           >  1:5729340/1‑91 (MQ=255)
                                                              gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                          <  2:3638033/93‑1 (MQ=255)
                                                              gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                          >  1:1455487/1‑93 (MQ=255)
                                                              gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                          >  1:2255213/1‑93 (MQ=255)
                                                              gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                          <  1:722771/93‑1 (MQ=255)
                                                               accagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCt                         <  1:1044498/93‑1 (MQ=255)
                                                                 cacacTCGCCAGAAAAAAAGCAAGCTCTGTCAATATCTCATCCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCttt                       <  2:3822327/90‑1 (MQ=255)
                                                                  agacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTg                      >  1:4050707/1‑93 (MQ=255)
                                                                   gacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGa                     >  2:185382/1‑93 (MQ=255)
                                                                      tCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCa                  >  1:1400864/1‑93 (MQ=255)
                                                                      tCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCa                  <  1:4294056/93‑1 (MQ=255)
                                                                        gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc                <  1:1357544/93‑1 (MQ=255)
                                                                        gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc                <  1:2161634/93‑1 (MQ=255)
                                                                         ccAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCa               >  2:3194256/1‑93 (MQ=255)
                                                                         ccAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCa               >  1:4538658/1‑93 (MQ=255)
                                                                         ccAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCa               >  1:5185464/1‑93 (MQ=255)
                                                                          cAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAg              <  2:4883290/93‑1 (MQ=255)
                                                                          cAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAg              <  2:2162384/93‑1 (MQ=255)
                                                                           aGCAAAAAAGCAAGCGCTGTCAATAACTCCCCCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGc             <  1:3745944/93‑1 (MQ=255)
                                                                           aGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGc             >  1:5123770/1‑93 (MQ=255)
                                                                             cAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCa           >  2:2319920/1‑93 (MQ=255)
                                                                               aaaaaGCAAGCGCGGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAgg         <  1:1922543/93‑1 (MQ=255)
                                                                                aaaaGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGga        >  1:4329928/1‑93 (MQ=255)
                                                                                 aaaGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGgag       <  2:2636696/93‑1 (MQ=255)
                                                                                  aagcaagcGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGgaga      >  1:3197388/1‑93 (MQ=255)
                                                                                   aGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGaa     >  1:2768547/1‑93 (MQ=255)
                                                                                    gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc    >  2:1696517/1‑93 (MQ=255)
                                                                                    gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc    <  1:1186592/93‑1 (MQ=255)
                                                                                    gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc    <  1:351144/93‑1 (MQ=255)
                                                                                    gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc    <  2:4381783/93‑1 (MQ=255)
                                                                                    gCAAGCGCGGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc    <  2:4162366/93‑1 (MQ=255)
                                                                                     cAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACa   >  2:3513443/1‑93 (MQ=255)
                                                                                      aaGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAg  >  1:4114642/1‑93 (MQ=255)
                                                                                         |                                                                                         
CTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAG  >  NC_000913/3111951‑3112129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGCG  >  NC_000913/3111947‑3112131
                                                                                             |                                                                                           
GACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGC                                                                                             <  SRR4302533.692067/94‑1 (MQ=60)
GACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGC                                                                                             <  SRR4302533.4097721/94‑1 (MQ=60)
GACACTCGCGGCAATCAGCCAGCCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGC                                                                                             <  SRR4302533.2405583/94‑1 (MQ=60)
    CTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTG                                                                                         >  SRR4302533.3788809/1‑94 (MQ=60)
      CGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTC                                                                                       >  SRR4302533.4632165/1‑94 (MQ=60)
      CGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTC                                                                                       <  SRR4302533.4896435/94‑1 (MQ=60)
       GCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCA                                                                                      >  SRR4302533.5108850/1‑94 (MQ=60)
        CGGAAATCAGCCAGGCGGATAATATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAA                                                                                     <  SRR4302533.5718408/94‑1 (MQ=60)
         GGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAAT                                                                                    >  SRR4302533.2338543/1‑94 (MQ=60)
           CAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAA                                                                                  >  SRR4302533.2926363/1‑94 (MQ=60)
           CAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAA                                                                                  >  SRR4302533.5047256/1‑94 (MQ=60)
            AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                                 >  SRR4302533.4204429/1‑94 (MQ=60)
            AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                                 <  SRR4302533.5930169/94‑1 (MQ=60)
            AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                                 >  SRR4302533.1127083/1‑94 (MQ=60)
            AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                                 >  SRR4302533.3845692/1‑94 (MQ=60)
            AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                                 >  SRR4302533.3522129/1‑94 (MQ=60)
             ATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACT                                                                                >  SRR4302533.2184896/1‑94 (MQ=60)
             ATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACT                                                                                <  SRR4302533.382497/94‑1 (MQ=60)
              TCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTC                                                                               >  SRR4302533.6299296/1‑94 (MQ=60)
              TCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTC                                                                               <  SRR4302533.4928657/94‑1 (MQ=60)
              ACAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTC                                                                               <  SRR4302533.6113322/94‑1 (MQ=60)
               CAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCC                                                                              <  SRR4302533.5708187/94‑1 (MQ=60)
               CAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCC                                                                              >  SRR4302533.1298773/1‑94 (MQ=60)
               CAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCC                                                                              <  SRR4302533.1932619/94‑1 (MQ=60)
                AGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCA                                                                             <  SRR4302533.5649371/94‑1 (MQ=60)
                AGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCA                                                                             >  SRR4302533.4266821/1‑94 (MQ=60)
                 GCCAGGCGGATAATATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCAC                                                                            <  SRR4302533.3913/94‑1 (MQ=60)
                  CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                           >  SRR4302533.5845659/1‑94 (MQ=60)
                  CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                           >  SRR4302533.1635320/1‑94 (MQ=60)
                   CAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGNNAGCGCTGTCAATAACNCCANCA                                                                          >  SRR4302533.4625680/1‑94 (MQ=60)
                   CAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCA                                                                          >  SRR4302533.349729/1‑94 (MQ=60)
                   CAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCA                                                                          <  SRR4302533.5504135/94‑1 (MQ=60)
                   CAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCA                                                                          <  SRR4302533.5136607/94‑1 (MQ=60)
                    AGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAG                                                                         <  SRR4302533.1286833/94‑1 (MQ=60)
                    AGGCGGATAATATCACCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAG                                                                         <  SRR4302533.1211422/94‑1 (MQ=60)
                      GCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCG                                                                       >  SRR4302533.1559599/1‑94 (MQ=60)
                       GGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGG                                                                      <  SRR4302533.1439852/94‑1 (MQ=60)
                       CGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGG                                                                      >  SRR4302533.710405/1‑94 (MQ=60)
                         GAAAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGAT                                                                    <  SRR4302533.4800992/94‑1 (MQ=60)
                          ATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATA                                                                   <  SRR4302533.3699343/94‑1 (MQ=60)
                          ATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATA                                                                   >  SRR4302533.1111920/1‑94 (MQ=60)
                           TAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAA                                                                  <  SRR4302533.5193016/94‑1 (MQ=60)
                           TAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAA                                                                  <  SRR4302533.90148/94‑1 (MQ=60)
                           TAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAA                                                                  <  SRR4302533.3504377/94‑1 (MQ=60)
                            AATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAC                                                                 <  SRR4302533.1687873/94‑1 (MQ=60)
                             ATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACG                                                                <  SRR4302533.4198275/94‑1 (MQ=60)
                             ATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACG                                                                <  SRR4302533.4334170/94‑1 (MQ=60)
                               ATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCT                                                              >  SRR4302533.1150267/1‑94 (MQ=60)
                                 CATCACCGCCTGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTG                                                            <  SRR4302533.680795/94‑1 (MQ=60)
                                   TCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCT                                                          <  SRR4302533.4970878/94‑1 (MQ=60)
                                    CCCCGCGAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTG                                                         <  SRR4302533.3425908/94‑1 (MQ=60)
                                     CCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGA                                                        <  SRR4302533.2680804/94‑1 (MQ=60)
                                      CCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAT                                                       <  SRR4302533.1555809/94‑1 (MQ=60)
                                       CGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATT                                                      <  SRR4302533.1896076/94‑1 (MQ=60)
                                         CCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTT                                                    <  SRR4302533.4845351/94‑1 (MQ=60)
                                          CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                   >  SRR4302533.2138581/1‑94 (MQ=60)
                                           AGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCG                                                  <  SRR4302533.1962758/94‑1 (MQ=60)
                                              CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCT                                               >  SRR4302533.1371824/1‑94 (MQ=60)
                                               CCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTG                                              >  SRR4302533.736868/1‑94 (MQ=60)
                                               CCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTG                                              <  SRR4302533.2186429/94‑1 (MQ=60)
                                                CCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGAAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGA                                             >  SRR4302533.6041634/1‑94 (MQ=60)
                                                  ATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACA                                           >  SRR4302533.6105998/1‑94 (MQ=60)
                                                  ATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACA                                           <  SRR4302533.2634612/94‑1 (MQ=60)
                                                   TCCACTTTCTGGCCAGACCAGACGCGCCAGCAAAAAAGCAAGCGCCGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAG                                          <  SRR4302533.1778409/94‑1 (MQ=60)
                                                    CCGCTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                         <  SRR4302533.1462803/94‑1 (MQ=60)
                                                    CCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                         >  SRR4302533.198397/1‑94 (MQ=60)
                                                    CCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                         >  SRR4302533.2784402/1‑94 (MQ=60)
                                                     CACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTC                                        <  SRR4302533.6181039/94‑1 (MQ=60)
                                                     CACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTC                                        <  SRR4302533.4906820/94‑1 (MQ=60)
                                                     CACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTC                                        >  SRR4302533.3684879/1‑94 (MQ=60)
                                                        TTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCG                                     <  SRR4302533.5792015/94‑1 (MQ=60)
                                                          TCTGGCCAGACCAGACTCGCTAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGC                                   <  SRR4302533.4231486/94‑1 (MQ=60)
                                                           CTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCA                                  <  SRR4302533.1249311/94‑1 (MQ=60)
                                                            cgccgAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTCTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAG                                 <  SRR4302533.2346023/89‑1 (MQ=60)
                                                             GGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGC                                <  SRR4302533.3697949/94‑1 (MQ=60)
                                                              GCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCG                               <  SRR4302533.1690011/94‑1 (MQ=60)
                                                              GCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCG                               >  SRR4302533.3602963/1‑94 (MQ=60)
                                                              GCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCG                               <  SRR4302533.413368/94‑1 (MQ=60)
                                                               CCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGC                              >  SRR4302533.2473266/1‑94 (MQ=60)
                                                               CCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGC                              >  SRR4302533.145096/1‑94 (MQ=60)
                                                                CAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCC                             >  SRR4302533.2667626/1‑94 (MQ=60)
                                                                CAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGAGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAccgac                             >  SRR4302533.5763937/1‑89 (MQ=60)
                                                                 AGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCC                            >  SRR4302533.2269162/1‑94 (MQ=60)
                                                                 AGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCC                            >  SRR4302533.1464261/1‑94 (MQ=60)
                                                                  GACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCT                           <  SRR4302533.727045/94‑1 (MQ=60)
                                                                  GACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCT                           <  SRR4302533.3536182/94‑1 (MQ=60)
                                                                   ACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTT                          <  SRR4302533.1050638/94‑1 (MQ=60)
                                                                   ACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTT                          <  SRR4302533.4784518/94‑1 (MQ=60)
                                                                    CCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTT                         <  SRR4302533.4046301/94‑1 (MQ=60)
                                                                    CCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTT                         <  SRR4302533.2627911/94‑1 (MQ=60)
                                                                    CCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTT                         >  SRR4302533.4368039/1‑94 (MQ=60)
                                                                     CAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTG                        >  SRR4302533.4075383/1‑94 (MQ=60)
                                                                     CAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTG                        <  SRR4302533.1150023/94‑1 (MQ=60)
                                                                      AGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGA                       <  SRR4302533.4322501/94‑1 (MQ=60)
                                                                        ACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGC                     >  SRR4302533.3718726/1‑94 (MQ=60)
                                                                        ACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGC                     >  SRR4302533.3886609/1‑94 (MQ=60)
                                                                         CTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCTCCAGCGGATAACGCTTGCTGATTTTCTCCTGACAGTCGCGGCAGCGCCCTTTGAGCA                    <  SRR4302533.886930/94‑1 (MQ=60)
                                                                         CTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCA                    >  SRR4302533.2658652/1‑94 (MQ=60)
                                                                         CTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCA                    >  SRR4302533.1409309/1‑94 (MQ=60)
                                                                          TCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCAT                   <  SRR4302533.4319882/94‑1 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 <  SRR4302533.5031306/94‑1 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 <  SRR4302533.6148694/94‑1 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 >  SRR4302533.5216764/1‑94 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 <  SRR4302533.2174939/94‑1 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 <  SRR4302533.1365704/94‑1 (MQ=60)
                                                                            GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA                 >  SRR4302533.4566137/1‑94 (MQ=60)
                                                                             GCAGCAAAAAAGCAAGCGCTGTCATTACCTCCACCAGCGGATATCGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAG                <  SRR4302533.5361359/94‑1 (MQ=60)
                                                                             CCAGCAAAAAAGCAAGCGCTGTCATTACCTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAG                <  SRR4302533.2329552/94‑1 (MQ=60)
                                                                              CAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGC               <  SRR4302533.2182634/94‑1 (MQ=60)
                                                                              CAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGC               >  SRR4302533.5154724/1‑94 (MQ=60)
                                                                               AGCAAAAAAGCAAGCGCTGTCAATAACTCCCCCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCC              <  SRR4302533.3768913/94‑1 (MQ=60)
                                                                               AGCAAAAAAGCAAGCGCTGTCAATAACTCCCCCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCC              <  SRR4302533.2300288/94‑1 (MQ=60)
                                                                               AGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCC              >  SRR4302533.1949196/1‑94 (MQ=60)
                                                                                  AAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGG           >  SRR4302533.309496/1‑94 (MQ=60)
                                                                                  AAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGG           >  SRR4302533.573787/1‑94 (MQ=60)
                                                                                  AAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGG           <  SRR4302533.2190206/94‑1 (MQ=60)
                                                                                   AAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGA          >  SRR4302533.4356013/1‑94 (MQ=60)
                                                                                   AAAAAGCAAGCGCGGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGA          <  SRR4302533.1934277/94‑1 (MQ=60)
                                                                                    AAAATCAAGCGCTGTCAATAGCTCAACCAGCGGATAACGCTTGCTGATTTTCACCTGACAGTCGCGGCAGCGCCCTTTGGGCATCAGCCAGGAG         <  SRR4302533.1712482/94‑1 (MQ=60)
                                                                                    AAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAG         <  SRR4302533.5457596/94‑1 (MQ=60)
                                                                                    AAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAG         <  SRR4302533.6142027/94‑1 (MQ=60)
                                                                                    AAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAG         >  SRR4302533.4534016/1‑94 (MQ=60)
                                                                                     AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA        >  SRR4302533.4735320/1‑94 (MQ=60)
                                                                                     AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA        >  SRR4302533.3217066/1‑94 (MQ=60)
                                                                                      AAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAA       >  SRR4302533.2785532/1‑94 (MQ=60)
                                                                                      AAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAA       >  SRR4302533.724030/1‑94 (MQ=60)
                                                                                      AAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAA       <  SRR4302533.40068/94‑1 (MQ=60)
                                                                                        GCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA     <  SRR4302533.2435933/94‑1 (MQ=60)
                                                                                        GCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA     <  SRR4302533.353285/94‑1 (MQ=60)
                                                                                        GCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA     <  SRR4302533.1193713/94‑1 (MQ=60)
                                                                                         CAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAG    >  SRR4302533.2471853/1‑94 (MQ=60)
                                                                                         CAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAG    >  SRR4302533.4139645/1‑94 (MQ=60)
                                                                                          AAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCCGGAGAACAGC   >  SRR4302533.2054292/1‑94 (MQ=60)
                                                                                          AAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGC   <  SRR4302533.5775877/94‑1 (MQ=60)
                                                                                          AAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGC   <  SRR4302533.14435/94‑1 (MQ=60)
                                                                                          AAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACACC   >  SRR4302533.3546672/1‑94 (MQ=60)
                                                                                           AGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGCG  <  SRR4302533.217624/94‑1 (MQ=60)
                                                                                           AGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGCG  <  SRR4302533.4099206/94‑1 (MQ=60)
                                                                                           AGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGCG  >  SRR4302533.912822/1‑94 (MQ=60)
                                                                                             |                                                                                           
GACACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACAGCG  >  NC_000913/3111947‑3112131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑