Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,370,778 A→G V145A (GTA→GCA)  nanA ← N‑acetylneuraminate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,370,7780AG100.0% 747.3 / NA 232V145A (GTA→GCA) nanAN‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (134/98);  total (134/98)

Only 100 of 234 total aligned reads displayed.

TCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA  >  NC_000913/3370688‑3370867
                                                                                          |                                                                                         
    tCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCAc                                                                                     >  1:304045/1‑93 (MQ=255)
      tGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTc                                                                                   >  2:95072/1‑93 (MQ=255)
      tGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTc                                                                                   >  2:5088372/1‑93 (MQ=255)
      tGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTc                                                                                   <  1:4373600/93‑1 (MQ=255)
       gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  <  2:4810590/93‑1 (MQ=255)
       gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  2:3579414/1‑93 (MQ=255)
       gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  1:737427/1‑93 (MQ=255)
       gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  1:4578910/1‑93 (MQ=255)
       gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  1:251116/1‑93 (MQ=255)
         tAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAgg                                                                                <  1:6172664/93‑1 (MQ=255)
         tAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGGGTTGATCTGATGCAGGGTCAGTTTTGCCCCACTCAgg                                                                                >  2:1953480/1‑93 (MQ=255)
            agaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  1:3128748/1‑88 (MQ=255)
            agaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  <  2:3128748/88‑1 (MQ=255)
             gaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTg                                                                            >  1:3311303/1‑93 (MQ=255)
             gaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTg                                                                            <  2:4053422/93‑1 (MQ=255)
             gaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTg                                                                            >  1:2777148/1‑93 (MQ=255)
              aTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTgg                                                                           >  2:1638494/1‑93 (MQ=255)
               tCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGa                                                                          >  1:2254727/1‑93 (MQ=255)
                 gCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAt                                                                        <  1:1634009/93‑1 (MQ=255)
                  ccAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtg                                                                       >  1:337494/1‑93 (MQ=255)
                  ccAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtg                                                                       >  2:837789/1‑93 (MQ=255)
                    agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     >  1:4269810/1‑93 (MQ=255)
                    agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     >  1:289158/1‑93 (MQ=255)
                    agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     >  2:6096474/1‑93 (MQ=255)
                     gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    <  2:4713770/93‑1 (MQ=255)
                     gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    <  1:102456/93‑1 (MQ=255)
                     gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    >  1:201613/1‑93 (MQ=255)
                     gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    >  1:1709996/1‑93 (MQ=255)
                     gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    >  1:368954/1‑93 (MQ=255)
                      agGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttgt                                                                   <  1:5295794/93‑1 (MQ=255)
                       ggTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTa                                                                  <  1:531839/93‑1 (MQ=255)
                       ggTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTa                                                                  >  1:4312269/1‑93 (MQ=255)
                        gTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTac                                                                 >  1:1117952/1‑93 (MQ=255)
                          cTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTacac                                                               >  2:2171070/1‑93 (MQ=255)
                          cTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTacac                                                               >  2:1356712/1‑93 (MQ=255)
                           tGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAcacc                                                              <  2:2925351/93‑1 (MQ=255)
                            gTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAcacca                                                             >  1:1970371/1‑93 (MQ=255)
                              ttCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACaccacc                                                           <  1:1542882/93‑1 (MQ=255)
                               tCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACAccacca                                                          <  1:4645867/93‑1 (MQ=255)
                               tCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACAccacca                                                          >  1:4714756/1‑93 (MQ=255)
                                 aGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                                        <  1:3621575/93‑1 (MQ=255)
                                 aGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                                        <  1:4623335/93‑1 (MQ=255)
                                 aGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                                        <  2:1831575/93‑1 (MQ=255)
                                 aGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                                        <  1:2350070/93‑1 (MQ=255)
                                  gcgcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCg                                                       <  2:2686136/93‑1 (MQ=255)
                                   cgcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCgg                                                      <  1:1839971/93‑1 (MQ=255)
                                    gcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGc                                                     >  2:3901117/1‑93 (MQ=255)
                                      aCCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaa                                                   >  2:3717966/1‑93 (MQ=255)
                                      aCCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaa                                                   >  2:4431315/1‑93 (MQ=255)
                                      aCCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaa                                                   >  1:389148/1‑93 (MQ=255)
                                       ccTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaaa                                                  >  2:4668349/1‑93 (MQ=255)
                                       ccTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaaa                                                  <  1:5375198/93‑1 (MQ=255)
                                        cTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAc                                                 >  2:3406770/1‑93 (MQ=255)
                                         tACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAcc                                                <  2:5877925/93‑1 (MQ=255)
                                           cGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTCTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCAt                                              >  2:464966/1‑93 (MQ=255)
                                            gCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATc                                             <  2:5189477/93‑1 (MQ=255)
                                             ccAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCAtac                                            >  2:5347571/1‑91 (MQ=255)
                                               aGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgc                                          >  2:4881994/1‑93 (MQ=255)
                                               aGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgc                                          <  1:3799176/93‑1 (MQ=255)
                                                 gCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgccg                                        >  2:434101/1‑93 (MQ=255)
                                                   attGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      >  2:1139424/3‑93 (MQ=255)
                                                   aaTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      >  1:341104/1‑93 (MQ=255)
                                                   aaTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      <  2:5391260/93‑1 (MQ=255)
                                                   aaTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      >  1:5080460/1‑93 (MQ=255)
                                                     tGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                                    >  2:6066305/1‑93 (MQ=255)
                                                     tGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                                    >  1:4851136/1‑93 (MQ=255)
                                                       tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  <  1:2063739/93‑1 (MQ=255)
                                                       tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  <  1:884158/93‑1 (MQ=255)
                                                       tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  >  2:6012438/1‑93 (MQ=255)
                                                       tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  >  2:1426431/1‑93 (MQ=255)
                                                        aaCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaat                                 <  1:2632456/93‑1 (MQ=255)
                                                         aCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata                                >  1:1644555/1‑93 (MQ=255)
                                                           aaGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                              <  2:2025304/93‑1 (MQ=255)
                                                             gtgtgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTg                            >  1:3944614/1‑93 (MQ=255)
                                                               gtgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGcc                          <  1:399119/93‑1 (MQ=255)
                                                                 gtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg                        >  2:2386847/1‑93 (MQ=255)
                                                                 gtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg                        >  1:2808778/1‑93 (MQ=255)
                                                                   tgatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt                      <  2:3332446/93‑1 (MQ=255)
                                                                    gatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATa                     <  2:2034132/93‑1 (MQ=255)
                                                                    gatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATa                     >  1:408961/1‑93 (MQ=255)
                                                                    gatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATa                     <  1:2444061/93‑1 (MQ=255)
                                                                     atctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAg                    >  1:1169958/1‑93 (MQ=255)
                                                                      tctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGt                   >  1:1951784/1‑93 (MQ=255)
                                                                      tctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGt                   >  2:5619462/1‑93 (MQ=255)
                                                                        tgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGa                 <  1:3585800/93‑1 (MQ=255)
                                                                        tgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGa                 <  2:285742/93‑1 (MQ=255)
                                                                         gatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAt                >  1:3028093/1‑93 (MQ=255)
                                                                         gatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAt                <  1:5716073/93‑1 (MQ=255)
                                                                          atcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATc               >  2:3221535/1‑93 (MQ=255)
                                                                             cAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa            <  2:5128832/93‑1 (MQ=255)
                                                                             cAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa            <  2:3530152/93‑1 (MQ=255)
                                                                             cAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa            >  1:58263/1‑93 (MQ=255)
                                                                              aGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAg           >  2:360107/1‑93 (MQ=255)
                                                                               gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:4895327/1‑93 (MQ=255)
                                                                               gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:5478098/1‑93 (MQ=255)
                                                                               gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:5774461/1‑93 (MQ=255)
                                                                               gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  1:1760885/1‑93 (MQ=255)
                                                                                 gTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtgt        <  2:4569265/93‑1 (MQ=255)
                                                                                       tttGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCa  <  2:2610814/93‑1 (MQ=255)
                                                                                       tttGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCa  <  1:3315839/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA  >  NC_000913/3370688‑3370867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGC  >  NC_000913/3370687‑3370871
                                                                                           |                                                                                             
CTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCC                                                                                             <  SRR4302533.3101553/94‑1 (MQ=60)
CTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCC                                                                                             >  SRR4302533.174568/1‑94 (MQ=60)
CTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCC                                                                                             >  SRR4302533.1644427/1‑94 (MQ=60)
  CCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCC                                                                                           >  SRR4302533.4678222/1‑94 (MQ=60)
    ATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCAC                                                                                         >  SRR4302533.305902/1‑94 (MQ=60)
     TCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACT                                                                                        >  SRR4302533.1447758/1‑94 (MQ=60)
      CTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTC                                                                                       >  SRR4302533.1105055/1‑94 (MQ=60)
       TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                      <  SRR4302533.4400000/94‑1 (MQ=60)
       TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                      >  SRR4302533.4606661/1‑94 (MQ=60)
       TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                      >  SRR4302533.1679794/1‑94 (MQ=60)
       TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                      >  SRR4302533.252664/1‑94 (MQ=60)
       TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                      >  SRR4302533.741769/1‑94 (MQ=60)
        GATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAG                                                                                     <  SRR4302533.2834214/94‑1 (MQ=60)
         ATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGG                                                                                    >  SRR4302533.2041636/1‑94 (MQ=60)
          TAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGG                                                                                   <  SRR4302533.3958455/94‑1 (MQ=60)
          TAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGG                                                                                   <  SRR4302533.6209756/94‑1 (MQ=60)
           AGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGC                                                                                  <  SRR4302533.2612652/94‑1 (MQ=60)
            GAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGatac                                                                                 >  SRR4302533.3147982/1‑90 (MQ=60)
            GAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCT                                                                                 >  SRR4302533.163044/1‑94 (MQ=60)
            GAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCT                                                                                 <  SRR4302533.2311573/94‑1 (MQ=60)
            GAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCT                                                                                 <  SRR4302533.2974940/94‑1 (MQ=60)
            GAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCT                                                                                 <  SRR4302533.1854629/94‑1 (MQ=60)
             AGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTG                                                                                >  SRR4302533.2794209/1‑94 (MQ=60)
             AGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTG                                                                                >  SRR4302533.3331750/1‑94 (MQ=60)
              GATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGG                                                                               >  SRR4302533.3682092/1‑94 (MQ=60)
               ATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGA                                                                              >  SRR4302533.3400716/1‑94 (MQ=60)
               ATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGA                                                                              >  SRR4302533.2268671/1‑94 (MQ=60)
                TCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAA                                                                             >  SRR4302533.31660/1‑94 (MQ=60)
                 CGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAT                                                                            >  SRR4302533.4391766/1‑94 (MQ=60)
                  GCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATG                                                                           >  SRR4302533.339556/1‑94 (MQ=60)
                  GCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATG                                                                           <  SRR4302533.1643906/94‑1 (MQ=60)
                    CAGAGGTGTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                         <  SRR4302533.2062804/94‑1 (MQ=60)
                    CAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                         <  SRR4302533.1151569/94‑1 (MQ=60)
                    CAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                         >  SRR4302533.4295513/1‑94 (MQ=60)
                    CAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                         <  SRR4302533.2467618/94‑1 (MQ=60)
                    CAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                         >  SRR4302533.290922/1‑94 (MQ=60)
                     AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                        >  SRR4302533.1720359/1‑94 (MQ=60)
                     AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                        >  SRR4302533.371182/1‑94 (MQ=60)
                     AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                        >  SRR4302533.202893/1‑94 (MQ=60)
                      GAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGT                                                                       <  SRR4302533.103094/94‑1 (MQ=60)
                       AGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTA                                                                      <  SRR4302533.5327513/94‑1 (MQ=60)
                       AGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTA                                                                      >  SRR4302533.4338228/1‑94 (MQ=60)
                        GGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAC                                                                     >  SRR4302533.1124584/1‑94 (MQ=60)
                        GGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAC                                                                     <  SRR4302533.4832580/94‑1 (MQ=60)
                        GGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAC                                                                     <  SRR4302533.535002/94‑1 (MQ=60)
                         GTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACA                                                                    <  SRR4302533.2439323/94‑1 (MQ=60)
                           CTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACC                                                                  >  SRR4302533.3752458/1‑94 (MQ=60)
                            TGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCA                                                                 >  SRR4302533.1982426/1‑94 (MQ=60)
                              TTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACC                                                               >  SRR4302533.3931783/1‑94 (MQ=60)
                               TTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCA                                                              <  SRR4302533.1552175/94‑1 (MQ=60)
                               TTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCA                                                              >  SRR4302533.4743330/1‑94 (MQ=60)
                               TTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCA                                                              >  SRR4302533.3704479/1‑94 (MQ=60)
                                TCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCAT                                                             <  SRR4302533.4674023/94‑1 (MQ=60)
                                 CAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATC                                                            >  SRR4302533.4544829/1‑94 (MQ=60)
                                  AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                           <  SRR4302533.4651359/94‑1 (MQ=60)
                                  AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                           <  SRR4302533.3643861/94‑1 (MQ=60)
                                  AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                           >  SRR4302533.3468689/1‑94 (MQ=60)
                                  AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                           <  SRR4302533.958923/94‑1 (MQ=60)
                                  AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                           <  SRR4302533.2364607/94‑1 (MQ=60)
                                    CGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGC                                                         <  SRR4302533.1851191/94‑1 (MQ=60)
                                      CACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAA                                                       >  SRR4302533.391497/1‑94 (MQ=60)
                                      CACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAA                                                       >  SRR4302533.5757046/1‑94 (MQ=60)
                                        CCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAC                                                     <  SRR4302533.5407500/94‑1 (MQ=60)
                                         CTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACC                                                    >  SRR4302533.5147131/1‑94 (MQ=60)
                                         CTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACC                                                    >  SRR4302533.1680214/1‑94 (MQ=60)
                                             GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCC                                                >  SRR4302533.3056364/1‑94 (MQ=60)
                                             GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAACATCC                                                >  SRR4302533.1894029/1‑94 (MQ=60)
                                             GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACAACCATCGGCAAACCATCC                                                >  SRR4302533.4650691/1‑94 (MQ=60)
                                              CCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCG                                               <  SRR4302533.723816/94‑1 (MQ=60)
                                               CAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCTCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGC                                              >  SRR4302533.4114750/1‑94 (MQ=60)
                                               CAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGC                                              >  SRR4302533.4333868/1‑94 (MQ=60)
                                               CAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGC                                              >  SRR4302533.1489143/1‑94 (MQ=60)
                                                AGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCC                                             <  SRR4302533.2760410/94‑1 (MQ=60)
                                                AGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCC                                             >  SRR4302533.338373/1‑94 (MQ=60)
                                                AGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCC                                             <  SRR4302533.3822435/94‑1 (MQ=60)
                                                AGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCC                                             <  SRR4302533.3134325/94‑1 (MQ=60)
                                                 GGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCG                                            <  SRR4302533.6091437/94‑1 (MQ=60)
                                                  GCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACACAGGGCTGGAATGTTGTACACCACCAGCGGCAAACCATCCGCCGA                                           <  SRR4302533.2401291/94‑1 (MQ=60)
                                                   CAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAA                                          >  SRR4302533.343188/1‑94 (MQ=60)
                                                   CAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAA                                          >  SRR4302533.5111166/1‑94 (MQ=60)
                                                     ATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATC                                        >  SRR4302533.5393166/1‑94 (MQ=60)
                                                     ATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATC                                        >  SRR4302533.4880535/1‑94 (MQ=60)
                                                      TGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCA                                       >  SRR4302533.3302587/1‑94 (MQ=60)
                                                        TAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAAT                                     <  SRR4302533.889350/94‑1 (MQ=60)
                                                        TAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAAT                                     <  SRR4302533.2076461/94‑1 (MQ=60)
                                                         AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                    >  SRR4302533.1654512/1‑94 (MQ=60)
                                                         AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                    <  SRR4302533.2648636/94‑1 (MQ=60)
                                                          ACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAA                                   >  SRR4302533.2823533/1‑94 (MQ=60)
                                                           CAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAT                                  <  SRR4302533.4537881/94‑1 (MQ=60)
                                                            AAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATT                                 >  SRR4302533.2005620/1‑94 (MQ=60)
                                                             AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                                >  SRR4302533.3968676/1‑94 (MQ=60)
                                                             AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                                <  SRR4302533.5243536/94‑1 (MQ=60)
                                                             AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                                <  SRR4302533.4656217/94‑1 (MQ=60)
                                                                GTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCC                             <  SRR4302533.401530/94‑1 (MQ=60)
                                                                GTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCC                             <  SRR4302533.3020713/94‑1 (MQ=60)
                                                                 TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                            <  SRR4302533.2240285/94‑1 (MQ=60)
                                                                 TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                            >  SRR4302533.3333304/1‑94 (MQ=60)
                                                                 TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                            >  SRR4302533.2826062/1‑94 (MQ=60)
                                                                  GTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGA                           >  SRR4302533.3994524/1‑94 (MQ=60)
                                                                   TTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAT                          <  SRR4302533.3631427/94‑1 (MQ=60)
                                                                    TGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATA                         <  SRR4302533.2709709/94‑1 (MQ=60)
                                                                    TGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATA                         >  SRR4302533.411415/1‑94 (MQ=60)
                                                                     GATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAG                        >  SRR4302533.1176980/1‑94 (MQ=60)
                                                                     GATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAG                        <  SRR4302533.2459145/94‑1 (MQ=60)
                                                                     GATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAG                        >  SRR4302533.5058321/1‑94 (MQ=60)
                                                                      ATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGT                       <  SRR4302533.533736/94‑1 (MQ=60)
                                                                      ATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGT                       >  SRR4302533.1963680/1‑94 (MQ=60)
                                                                       TCTGATCCAGGGTCAGTCTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTG                      <  SRR4302533.467741/94‑1 (MQ=60)
                                                                         TGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAT                    <  SRR4302533.3607885/94‑1 (MQ=60)
                                                                         TGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAT                    >  SRR4302533.3046624/1‑94 (MQ=60)
                                                                          GATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATC                   <  SRR4302533.5750596/94‑1 (MQ=60)
                                                                           ATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCG                  >  SRR4302533.4032305/1‑94 (MQ=60)
                                                                            TCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGC                 >  SRR4302533.5567515/1‑94 (MQ=60)
                                                                            TCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGGGATCGC                 >  SRR4302533.42081/1‑94 (MQ=60)
                                                                             CCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA                >  SRR4302533.58648/1‑94 (MQ=60)
                                                                             CCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA                <  SRR4302533.799738/94‑1 (MQ=60)
                                                                             CCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA                >  SRR4302533.1977482/1‑94 (MQ=60)
                                                                               AGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGT              >  SRR4302533.1771616/1‑94 (MQ=60)
                                                                                 GGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT            >  SRR4302533.3431200/1‑94 (MQ=60)
                                                                                 GGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT            <  SRR4302533.5583335/94‑1 (MQ=60)
                                                                                       TTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA      <  SRR4302533.1845901/94‑1 (MQ=60)
                                                                                        TTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAA     <  SRR4302533.6103919/94‑1 (MQ=60)
                                                                                        TTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAA     <  SRR4302533.3336313/94‑1 (MQ=60)
                                                                                        TTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAA     <  SRR4302533.3833702/94‑1 (MQ=60)
                                                                                           GCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGC  <  SRR4302533.2171347/94‑1 (MQ=60)
                                                                                           GCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGC  <  SRR4302533.308104/94‑1 (MQ=60)
                                                                                           |                                                                                             
CTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGC  >  NC_000913/3370687‑3370871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑