Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,410,192 T→C intergenic (+18/‑66) b3262 → / → yhdU putative methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,1920TC100.0% 876.5 / NA 269intergenic (+18/‑66)b3262/yhdUputative methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (141/128);  total (141/128)

Only 100 of 281 total aligned reads displayed.

AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410102‑3410281
                                                                                          |                                                                                         
aaaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGctt                                                                                         <  1:2979267/93‑1 (MQ=255)
aaaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGctt                                                                                         >  1:553937/1‑93 (MQ=255)
 aaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGcttt                                                                                        <  2:6208810/93‑1 (MQ=255)
  aaagaaagACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTc                                                                                       <  1:5363222/93‑1 (MQ=255)
   aagaaagaCCGGCAACCTGTCAAAACGAAGCCTGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCa                                                                                      <  1:326456/93‑1 (MQ=255)
   aagaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCa                                                                                      >  2:1287308/1‑93 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    <  2:4290927/93‑1 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    <  1:2904264/93‑1 (MQ=255)
       aagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGatt                                                                                  <  1:3671114/93‑1 (MQ=255)
        agaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGatta                                                                                 >  2:1181317/1‑93 (MQ=255)
        agaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGatta                                                                                 >  1:4460885/1‑93 (MQ=255)
         gaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattat                                                                                >  2:286624/1‑93 (MQ=255)
         gaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattat                                                                                >  1:5958944/1‑93 (MQ=255)
          aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               <  2:2492787/93‑1 (MQ=255)
           ccGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTc                                                                              >  1:2406518/1‑93 (MQ=255)
            cGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCa                                                                             >  2:3226552/1‑93 (MQ=255)
             ggCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAt                                                                            <  1:4194410/93‑1 (MQ=255)
             ggCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAt                                                                            >  2:3860854/1‑93 (MQ=255)
             ggCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGACGTGTAAGCCTGGCTTTCAGATTATTCAt                                                                            >  1:5850843/1‑93 (MQ=255)
               cAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTTGCTTTCAGATTATTCAttt                                                                          <  2:6083887/93‑1 (MQ=255)
               cAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAttt                                                                          <  2:2028207/93‑1 (MQ=255)
                aaCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTc                                                                         <  1:5446345/93‑1 (MQ=255)
                aaCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTc                                                                         >  2:2439074/1‑93 (MQ=255)
                  ccTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgt                                                                       <  1:3147674/93‑1 (MQ=255)
                    tGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgtgt                                                                     >  2:2772607/1‑93 (MQ=255)
                    tGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgtgt                                                                     >  2:2120639/1‑93 (MQ=255)
                     gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  1:2338536/93‑1 (MQ=255)
                     gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  2:5522277/93‑1 (MQ=255)
                     gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  2:5750853/93‑1 (MQ=255)
                     gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  2:3733309/93‑1 (MQ=255)
                      tCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTTATTTAGTGtaa                                                                   >  2:5484623/1‑92 (MQ=255)
                      tCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGtat                                                                   >  1:6103042/1‑93 (MQ=255)
                      tCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGtat                                                                   >  1:346233/1‑93 (MQ=255)
                         aaaCGAAGCCGGTTAAGCTAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                <  1:3972340/93‑1 (MQ=255)
                           aCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTc                                                              >  2:2594037/1‑93 (MQ=255)
                              aaGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTg                                                           >  1:360869/1‑93 (MQ=255)
                               aGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGc                                                          <  2:3292035/93‑1 (MQ=255)
                                gCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGcc                                                         >  1:3707074/1‑93 (MQ=255)
                                  cGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAg                                                       >  2:1814833/1‑93 (MQ=255)
                                  cGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAg                                                       >  2:668690/1‑93 (MQ=255)
                                   ggTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGa                                                      >  2:3621925/1‑93 (MQ=255)
                                   ggTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGa                                                      >  1:5594213/1‑93 (MQ=255)
                                      tAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACtt                                                   <  2:4040523/93‑1 (MQ=255)
                                       aaGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  <  2:2661107/93‑1 (MQ=255)
                                       aaGAGAAGTTGCCCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  <  2:5362943/93‑1 (MQ=255)
                                        aGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTgg                                                 <  1:4419149/93‑1 (MQ=255)
                                         gCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGt                                                >  2:3379828/1‑93 (MQ=255)
                                         gCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGt                                                <  2:760477/93‑1 (MQ=255)
                                         gCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGt                                                >  1:5076421/1‑93 (MQ=255)
                                           gAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTa                                              >  1:1647625/1‑93 (MQ=255)
                                           gAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTa                                              >  1:2303858/1‑93 (MQ=255)
                                             aGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTaaa                                            <  1:4615973/93‑1 (MQ=255)
                                              gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           <  1:1307607/93‑1 (MQ=255)
                                              gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           <  1:410450/93‑1 (MQ=255)
                                              gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           <  2:1855441/93‑1 (MQ=255)
                                              gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTATCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           >  1:868934/1‑93 (MQ=255)
                                               ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          >  2:2880557/1‑93 (MQ=255)
                                               ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          <  2:5016818/93‑1 (MQ=255)
                                                 gACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATg                                        <  1:1932839/93‑1 (MQ=255)
                                                     ccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcaca                                    <  2:467550/93‑1 (MQ=255)
                                                     ccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcaca                                    <  2:3239231/93‑1 (MQ=255)
                                                      cGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAg                                   >  1:3836606/1‑93 (MQ=255)
                                                      cGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAg                                   <  1:2727023/93‑1 (MQ=255)
                                                       gATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAgg                                  >  1:5910503/1‑93 (MQ=255)
                                                        aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 <  2:4655673/93‑1 (MQ=255)
                                                         tctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCt                                >  1:6264686/1‑93 (MQ=255)
                                                          ctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               <  1:1060981/93‑1 (MQ=255)
                                                          ctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               <  1:1145419/93‑1 (MQ=255)
                                                           tcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCa                              <  1:3531175/93‑1 (MQ=255)
                                                           tcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCa                              >  1:1755577/1‑93 (MQ=255)
                                                            cATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAg                             <  1:2593906/93‑1 (MQ=255)
                                                            cATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAg                             >  2:3170977/1‑93 (MQ=255)
                                                            cATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAg                             >  1:3912583/1‑93 (MQ=255)
                                                             aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            >  1:2124485/1‑93 (MQ=255)
                                                             aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            >  1:4352576/1‑93 (MQ=255)
                                                             aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTCCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            <  2:1432235/93‑1 (MQ=255)
                                                              ttACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGt                           >  2:5950315/1‑93 (MQ=255)
                                                                aCAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTaa                         >  2:6175447/1‑93 (MQ=255)
                                                                 cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        <  1:657474/93‑1 (MQ=255)
                                                                  aaaGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATg                       <  2:4928211/93‑1 (MQ=255)
                                                                    aGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAt                     >  2:308798/1‑93 (MQ=255)
                                                                     gTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAtt                    <  1:1012247/93‑1 (MQ=255)
                                                                      tAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTc                   >  1:4711258/1‑93 (MQ=255)
                                                                      tAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTc                   <  2:1066640/93‑1 (MQ=255)
                                                                          ggATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCaa               <  1:5205619/93‑1 (MQ=255)
                                                                           gATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAg              >  1:3227169/1‑93 (MQ=255)
                                                                             tgtgTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTa            >  2:4742963/1‑93 (MQ=255)
                                                                              gtgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAt           >  2:1603177/1‑93 (MQ=255)
                                                                                gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         <  1:3830330/93‑1 (MQ=255)
                                                                                gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         >  2:5044010/1‑93 (MQ=255)
                                                                                gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         <  1:4548604/93‑1 (MQ=255)
                                                                                 tAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtgg        <  1:5689412/93‑1 (MQ=255)
                                                                                    gCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtgg     >  2:2345625/1‑93 (MQ=255)
                                                                                    gCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtgg     >  1:1721872/1‑93 (MQ=255)
                                                                                    gCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtgg     >  2:1791986/1‑93 (MQ=255)
                                                                                     ccTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGc    <  1:1784213/93‑1 (MQ=255)
                                                                                      cTGGCTTTCAGATTCTTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   >  2:3918405/1‑93 (MQ=255)
                                                                                      cTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   <  2:5282524/93‑1 (MQ=255)
                                                                                       tGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCtc  >  1:2773102/1‑93 (MQ=255)
                                                                                       tGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCtc  >  2:3490524/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410102‑3410281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410099‑3410285
                                                                                             |                                                                                             
GAAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGC                                                                                               >  SRR4302533.4376712/1‑94 (MQ=60)
 AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              <  SRR4302533.485637/94‑1 (MQ=60)
 AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              <  SRR4302533.6292465/94‑1 (MQ=60)
  AAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTT                                                                                             >  SRR4302533.557221/1‑94 (MQ=60)
   AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTT                                                                                            <  SRR4302533.2997497/94‑1 (MQ=60)
   AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTT                                                                                            <  SRR4302533.1125162/94‑1 (MQ=60)
    AAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTC                                                                                           <  SRR4302533.31928/94‑1 (MQ=60)
     AAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCA                                                                                          <  SRR4302533.4554467/94‑1 (MQ=60)
     AAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCA                                                                                          >  SRR4302533.3582288/1‑94 (MQ=60)
     AAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCA                                                                                          <  SRR4302533.5395435/94‑1 (MQ=60)
      AAGAAAGACCGGCAACCTGTCAAAACGAAGCCTGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAG                                                                                         <  SRR4302533.328442/94‑1 (MQ=60)
       AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGA                                                                                        <  SRR4302533.2715135/94‑1 (MQ=60)
        GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       <  SRR4302533.2922101/94‑1 (MQ=60)
        GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       <  SRR4302533.1922711/94‑1 (MQ=60)
        GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       >  SRR4302533.6165086/1‑94 (MQ=60)
          tagtCCGGCAACCTGTCAAAACGAAGCCGGTTTAGCGAAGTTGACCCCGATCTCATTTCAAAGTATATGATGTGTAAGCCTGGCTTTCAGtttt                                                                                     >  SRR4302533.6333233/5‑90 (MQ=60)
          AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     <  SRR4302533.3693677/94‑1 (MQ=60)
          AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302533.4487868/1‑94 (MQ=60)
          AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302533.4200016/1‑94 (MQ=60)
           AGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTAT                                                                                    >  SRR4302533.5994989/1‑94 (MQ=60)
            GACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATT                                                                                   >  SRR4302533.5124065/1‑94 (MQ=60)
             ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  >  SRR4302533.2421383/1‑94 (MQ=60)
             ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  >  SRR4302533.5912115/1‑94 (MQ=60)
             ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  >  SRR4302533.656305/1‑94 (MQ=60)
               CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGCCTTTCAGATTATTCAT                                                                                >  SRR4302533.2036155/1‑94 (MQ=60)
               CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGACGTGTAAGCCTGGCTTTCAGATTATTCAT                                                                                >  SRR4302533.5886216/1‑94 (MQ=60)
                GGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATT                                                                               >  SRR4302533.3093376/1‑94 (MQ=60)
                GGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATT                                                                               >  SRR4302533.2760171/1‑94 (MQ=60)
                GGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATT                                                                               <  SRR4302533.4219798/94‑1 (MQ=60)
                 GCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTT                                                                              <  SRR4302533.3315996/94‑1 (MQ=60)
                  CAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTC                                                                             >  SRR4302533.4446882/1‑94 (MQ=60)
                   AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            <  SRR4302533.5989768/94‑1 (MQ=60)
                   AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            <  SRR4302533.441351/94‑1 (MQ=60)
                   AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            <  SRR4302533.5479156/94‑1 (MQ=60)
                     CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          <  SRR4302533.3167027/94‑1 (MQ=60)
                     CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          >  SRR4302533.2503639/1‑94 (MQ=60)
                     CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          >  SRR4302533.683075/1‑94 (MQ=60)
                      CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         <  SRR4302533.4704544/94‑1 (MQ=60)
                      CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302533.4096441/1‑94 (MQ=60)
                      CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302533.400501/1‑94 (MQ=60)
                      CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302533.957343/1‑94 (MQ=60)
                       TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        >  SRR4302533.3943566/1‑94 (MQ=60)
                       TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        >  SRR4302533.5898119/1‑94 (MQ=60)
                        GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       >  SRR4302533.6139884/1‑94 (MQ=60)
                        GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       <  SRR4302533.2353016/94‑1 (MQ=60)
                        GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       >  SRR4302533.348345/1‑94 (MQ=60)
                          CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302533.1666855/94‑1 (MQ=60)
                          CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302533.5511232/94‑1 (MQ=60)
                          CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302533.2294048/94‑1 (MQ=60)
                          CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     >  SRR4302533.3339193/1‑94 (MQ=60)
                           AAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGTCTTTCAGATTATTCATTTCGTGTATATT                                                                    <  SRR4302533.367468/94‑1 (MQ=60)
                           AAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATT                                                                    >  SRR4302533.3865303/1‑94 (MQ=60)
                            AAACGAAGCCGGTTAAGCTAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   <  SRR4302533.3996530/94‑1 (MQ=60)
                            AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302533.5378805/1‑94 (MQ=60)
                            AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302533.4849229/1‑94 (MQ=60)
                             AACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTC                                                                  <  SRR4302533.5812107/94‑1 (MQ=60)
                             AACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTC                                                                  <  SRR4302533.5513301/94‑1 (MQ=60)
                              ACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCC                                                                 <  SRR4302533.5955342/94‑1 (MQ=60)
                              ACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCC                                                                 >  SRR4302533.3592325/1‑94 (MQ=60)
                              ACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCC                                                                 <  SRR4302533.4636808/94‑1 (MQ=60)
                               CGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCT                                                                <  SRR4302533.3723366/94‑1 (MQ=60)
                                GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTG                                                               >  SRR4302533.363065/1‑94 (MQ=60)
                                 AAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGC                                                              >  SRR4302533.1202950/1‑94 (MQ=60)
                                  AGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCC                                                             >  SRR4302533.3729842/1‑94 (MQ=60)
                                    CCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAG                                                           <  SRR4302533.5281590/94‑1 (MQ=60)
                                     CGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGA                                                          >  SRR4302533.5628008/1‑94 (MQ=60)
                                      GGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAC                                                         <  SRR4302533.1323451/94‑1 (MQ=60)
                                           AGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGT                                                    >  SRR4302533.2548442/1‑94 (MQ=60)
                                           AGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGT                                                    >  SRR4302533.5107107/1‑94 (MQ=60)
                                           AGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGT                                                    <  SRR4302533.4445868/94‑1 (MQ=60)
                                           AGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGT                                                    >  SRR4302533.1930332/1‑94 (MQ=60)
                                           AGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGT                                                    <  SRR4302533.3263857/94‑1 (MQ=60)
                                             CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  >  SRR4302533.1657605/1‑94 (MQ=60)
                                             CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  >  SRR4302533.2318128/1‑94 (MQ=60)
                                               AAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAA                                                >  SRR4302533.4520798/1‑94 (MQ=60)
                                                AGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAC                                               <  SRR4302533.2340334/94‑1 (MQ=60)
                                                AGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAC                                               <  SRR4302533.4643926/94‑1 (MQ=60)
                                                AGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTATCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAC                                               >  SRR4302533.874033/1‑94 (MQ=60)
                                                 GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACA                                              <  SRR4302533.1315459/94‑1 (MQ=60)
                                                 GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACA                                              <  SRR4302533.412910/94‑1 (MQ=60)
                                                  TTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACAT                                             <  SRR4302533.3892584/94‑1 (MQ=60)
                                                    GACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGC                                           <  SRR4302533.1944629/94‑1 (MQ=60)
                                                     ACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCA                                          >  SRR4302533.4172524/1‑94 (MQ=60)
                                                      CCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCAC                                         >  SRR4302533.56180/1‑94 (MQ=60)
                                                       CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        >  SRR4302533.1783449/1‑94 (MQ=60)
                                                        CCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       <  SRR4302533.2397672/94‑1 (MQ=60)
                                                        CCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       >  SRR4302533.3060787/1‑94 (MQ=60)
                                                        CCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       >  SRR4302533.3860047/1‑94 (MQ=60)
                                                        CCGAGCACATTACAAACTAAAGGATGAATAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       <  SRR4302533.2695799/94‑1 (MQ=60)
                                                         CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      <  SRR4302533.4428689/94‑1 (MQ=60)
                                                         CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      >  SRR4302533.5946209/1‑94 (MQ=60)
                                                         CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      >  SRR4302533.3806639/1‑94 (MQ=60)
                                                         CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      <  SRR4302533.2743751/94‑1 (MQ=60)
                                                          GATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGC                                     >  SRR4302533.3674140/1‑94 (MQ=60)
                                                          GATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGC                                     <  SRR4302533.510766/94‑1 (MQ=60)
                                                           ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302533.6302127/1‑94 (MQ=60)
                                                            TCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTC                                   >  SRR4302533.21144/1‑94 (MQ=60)
                                                             CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  <  SRR4302533.1152255/94‑1 (MQ=60)
                                                             CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  >  SRR4302533.1766273/1‑94 (MQ=60)
                                                             CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  >  SRR4302533.2971827/1‑94 (MQ=60)
                                                             CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  >  SRR4302533.1766272/1‑94 (MQ=60)
                                                             CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  <  SRR4302533.1067216/94‑1 (MQ=60)
                                                              TCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAG                                 <  SRR4302533.3552937/94‑1 (MQ=60)
                                                              TCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAG                                 >  SRR4302533.3936479/1‑94 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                <  SRR4302533.2609844/94‑1 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                <  SRR4302533.4774670/94‑1 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                <  SRR4302533.1170969/94‑1 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                <  SRR4302533.3712372/94‑1 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302533.4378842/1‑94 (MQ=60)
                                                               CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302533.2137582/1‑94 (MQ=60)
                                                                 TTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTA                              >  SRR4302533.2865056/1‑94 (MQ=60)
                                                                 TTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTA                              <  SRR4302533.113443/94‑1 (MQ=60)
                                                                  TACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAA                             >  SRR4302533.6108223/1‑94 (MQ=60)
                                                                  TACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAA                             <  SRR4302533.1323508/94‑1 (MQ=60)
                                                                   ACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAT                            <  SRR4302533.4616581/94‑1 (MQ=60)
                                                                    CAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATG                           <  SRR4302533.2201720/94‑1 (MQ=60)
                                                                    CAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATG                           <  SRR4302533.661351/94‑1 (MQ=60)
                                                                      AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAT                         >  SRR4302533.3209053/1‑94 (MQ=60)
                                                                      AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAT                         <  SRR4302533.4267320/94‑1 (MQ=60)
                                                                       AGTAAAGGATGTGTAAGCCTGTCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGCCTTGGTTAAACATGCACAGGCTCAGGTAATGATT                        <  SRR4302533.5681510/94‑1 (MQ=60)
                                                                       AGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATT                        <  SRR4302533.5876924/94‑1 (MQ=60)
                                                                        GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       <  SRR4302533.2930572/94‑1 (MQ=60)
                                                                        GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       >  SRR4302533.4739812/1‑94 (MQ=60)
                                                                        GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       <  SRR4302533.1018170/94‑1 (MQ=60)
                                                                        GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       <  SRR4302533.5669112/94‑1 (MQ=60)
                                                                           AAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCA                    >  SRR4302533.5428707/1‑94 (MQ=60)
                                                                           AAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCA                    >  SRR4302533.3501018/1‑94 (MQ=60)
                                                                            AGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAA                   >  SRR4302533.6260022/1‑94 (MQ=60)
                                                                             GGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAG                  <  SRR4302533.5236986/94‑1 (MQ=60)
                                                                             GGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAG                  >  SRR4302533.3247064/1‑94 (MQ=60)
                                                                              GATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGT                 <  SRR4302533.916498/94‑1 (MQ=60)
                                                                                  TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             >  SRR4302533.5402242/1‑94 (MQ=60)
                                                                                   GTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGG            <  SRR4302533.815871/94‑1 (MQ=60)
                                                                                   GTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGG            <  SRR4302533.3853746/94‑1 (MQ=60)
                                                                                   GTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGG            <  SRR4302533.4576152/94‑1 (MQ=60)
                                                                                    TAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGT           <  SRR4302533.5723776/94‑1 (MQ=60)
                                                                                     AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          <  SRR4302533.175863/94‑1 (MQ=60)
                                                                                     AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGCG          <  SRR4302533.3804400/94‑1 (MQ=60)
                                                                                      AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         <  SRR4302533.2416358/94‑1 (MQ=60)
                                                                                      AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         <  SRR4302533.445901/94‑1 (MQ=60)
                                                                                      AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         >  SRR4302533.1732315/1‑94 (MQ=60)
                                                                                       GCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGC        >  SRR4302533.1029310/1‑94 (MQ=60)
                                                                                        CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       <  SRR4302533.1795061/94‑1 (MQ=60)
                                                                                        CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGGATTGGTGGCT       >  SRR4302533.1715796/1‑94 (MQ=60)
                                                                                         CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302533.2790124/1‑94 (MQ=60)
                                                                                         CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302533.2790123/1‑94 (MQ=60)
                                                                                         CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302533.3858004/1‑94 (MQ=60)
                                                                                         CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302533.3984713/1‑94 (MQ=60)
                                                                                          TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     <  SRR4302533.328066/94‑1 (MQ=60)
                                                                                          TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302533.656679/1‑94 (MQ=60)
                                                                                           GGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGT    >  SRR4302533.6188897/1‑94 (MQ=60)
                                                                                           GGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGT    >  SRR4302533.4072083/1‑94 (MQ=60)
                                                                                             CTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  SRR4302533.5813060/1‑94 (MQ=60)
                                                                                             CTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  <  SRR4302533.5672053/94‑1 (MQ=60)
                                                                                             |                                                                                             
GAAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410099‑3410285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑