Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,430,453	+C	coding (745/747 nt)	smf ←	putative Rossmann‑fold nucleotide‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,430,453	1	.	C	99.5%	689.2 / ‑3.9	215	coding (745/747 nt)	smf	putative Rossmann‑fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (125/89); total (126/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 238 total aligned reads displayed.

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539

gAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTTTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAgg                                                                                         >  2:380159/1‑93 (MQ=255)

   tttCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaagg‑caa                                                                                     >  2:6282168/1‑93 (MQ=255)

     tcttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaaa                                                                                   >  1:2933820/1‑93 (MQ=255)

       ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAc                                                                                  <  1:1312311/93‑1 (MQ=255)

       ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaaaac                                                                                 >  2:2403561/1‑91 (MQ=255)

        tcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCCAGGCAAAAACt                                                                                 >  1:2177617/1‑93 (MQ=255)

         ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACtt                                                                                <  2:1428297/93‑1 (MQ=255)

          cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtc                                                                               <  1:6040695/93‑1 (MQ=255)

          cGGTCCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGTCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtc                                                                               <  2:4324689/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             <  2:6216695/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             >  2:5244988/1‑93 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             >  1:6251441/1‑93 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             <  2:2242581/93‑1 (MQ=255)

            gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctc                                                                             <  2:2061909/93‑1 (MQ=255)

             ttCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCg                                                                            >  2:3286486/1‑93 (MQ=255)

              tCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGc                                                                           >  1:5385035/1‑93 (MQ=255)

               ccGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcc                                                                          <  2:114500/93‑1 (MQ=255)

                   cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTg                                                                      >  2:2897285/1‑93 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                     >  2:1572462/1‑93 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                     <  2:3750847/93‑1 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                     <  2:880671/93‑1 (MQ=255)

                    acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                     >  1:1136363/1‑93 (MQ=255)

                     caAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                    >  2:353614/1‑93 (MQ=255)

                     caAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                    >  1:5852544/1‑93 (MQ=255)

                       aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCa                                                                  >  2:4003097/1‑93 (MQ=255)

                       aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCa                                                                  >  1:4500142/1‑93 (MQ=255)

                         aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAg                                                                <  1:3201996/93‑1 (MQ=255)

                         aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAg                                                                >  1:2731883/1‑93 (MQ=255)

                          aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgc                                                               <  1:3603448/93‑1 (MQ=255)

                          aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgc                                                               >  1:4816733/1‑93 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  2:5586090/1‑93 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  2:93157/1‑93 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  1:4262911/1‑93 (MQ=255)

                             gCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                            >  2:3626164/1‑93 (MQ=255)

                              cACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGcac                                                           >  1:4320475/1‑93 (MQ=255)

                               aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  1:1496273/1‑93 (MQ=255)

                               aCCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGcaca                                                          >  2:468491/1‑93 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         >  1:4227599/1‑93 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         <  2:2643006/93‑1 (MQ=255)

                                ccTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACaa                                                         <  1:1607918/93‑1 (MQ=255)

                                 cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAc                                                        <  1:3514345/93‑1 (MQ=255)

                                  ttGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACg                                                       >  1:4198410/1‑93 (MQ=255)

                                  ttGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACg                                                       <  1:2797600/93‑1 (MQ=255)

                                   tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      >  2:4385713/1‑93 (MQ=255)

                                   tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      <  1:4766651/93‑1 (MQ=255)

                                   tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCAGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGt                                                      >  2:269965/1‑93 (MQ=255)

                                    gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                     >  2:4373826/1‑93 (MQ=255)

                                     ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGc                                                    <  1:3370723/93‑1 (MQ=255)

                                       ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTg                                                  <  2:5513968/93‑1 (MQ=255)

                                          ttaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCa                                               <  1:6056719/93‑1 (MQ=255)

                                            aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAcc                                             <  2:5998652/93‑1 (MQ=255)

                                            aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAcc                                             <  2:5751400/93‑1 (MQ=255)

                                            aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAcc                                             >  1:331405/1‑93 (MQ=255)

                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            <  1:2052108/93‑1 (MQ=255)

                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            <  2:4774126/93‑1 (MQ=255)

                                             aCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                            >  2:1310841/1‑93 (MQ=255)

                                              ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAg                                           >  2:1462893/1‑93 (MQ=255)

                                                aGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCg                                         >  2:4086728/1‑93 (MQ=255)

                                                aGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCg                                         >  2:1620386/1‑93 (MQ=255)

                                                 gTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGa                                        >  1:1751672/1‑93 (MQ=255)

                                                  tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGaa                                       >  2:5416203/1‑93 (MQ=255)

                                                  tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGaa                                       >  1:387386/1‑93 (MQ=255)

                                                   gAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAc                                      >  1:4634974/1‑93 (MQ=255)

                                                     ggCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCa                                    >  2:6087215/1‑93 (MQ=255)

                                                       cccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACt                                  <  2:4453232/93‑1 (MQ=255)

                                                         cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                >  1:5917177/1‑93 (MQ=255)

                                                          ttCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                               >  1:2381839/1‑93 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              <  2:409071/93‑1 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              >  2:3033329/1‑93 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                              >  1:1013698/1‑93 (MQ=255)

                                                           tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGNGCTGTCACCAGCGNACCACTACGc                              >  2:3082689/1‑93 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           >  1:3710911/1‑93 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  1:2562861/93‑1 (MQ=255)

                                                              cTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaaa                           <  2:2973179/93‑1 (MQ=255)

                                                               ttCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAg                          >  1:5193753/1‑93 (MQ=255)

                                                                tCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         >  1:4096522/1‑93 (MQ=255)

                                                                tCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         >  1:3941579/1‑93 (MQ=255)

                                                                tCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGc                         <  2:3233353/93‑1 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:2148683/1‑93 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       <  1:5080889/93‑1 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:1711490/1‑93 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  2:1249698/1‑93 (MQ=255)

                                                                  ccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                       >  1:3678624/1‑93 (MQ=255)

                                                                   cGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGc                      >  1:1900027/1‑93 (MQ=255)

                                                                    ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                     >  1:5629986/1‑93 (MQ=255)

                                                                           cTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacca              >  1:5092887/1‑93 (MQ=255)

                                                                           cTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacca              >  1:1445880/1‑93 (MQ=255)

                                                                            tATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccac             >  2:4900640/1‑93 (MQ=255)

                                                                             aTTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacc            <  1:1322867/93‑1 (MQ=255)

                                                                             aTTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacc            <  1:5761294/93‑1 (MQ=255)

                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           <  2:1001746/93‑1 (MQ=255)

                                                                              ttGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca           <  2:5710015/93‑1 (MQ=255)

                                                                                ggACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGt         <  1:4169552/93‑1 (MQ=255)

                                                                                 gACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        <  1:4896541/93‑1 (MQ=255)

                                                                                 gACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        >  1:2307094/1‑93 (MQ=255)

                                                                                 gACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa        >  1:34638/1‑93 (MQ=255)

                                                                                    cTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac     >  2:1850807/1‑93 (MQ=255)

                                                                                     tGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTCCGCAAAGCCGCTTCCCGCACCAGTacact    >  1:1270395/1‑92 (MQ=255)

                                                                                      ggcaaggcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  1:1176674/1‑93 (MQ=255)

                                                                                      ggcaaggcaaAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt   >  2:2819010/1‑93 (MQ=255)

GAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTT  >  NC_000913/3430361‑3430539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430362‑3430546

AATTTCTTCCGGTTCCGTCACACGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑CA                                                                                             <  SRR4302533.5108406/94‑1 (MQ=60)

  TTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑CAAA                                                                                           >  SRR4302533.6087878/1‑94 (MQ=60)

   TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑CAAAA                                                                                          >  SRR4302533.2951759/1‑94 (MQ=60)

   TTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑CAAAA                                                                                          >  SRR4302533.5871012/1‑94 (MQ=60)

     CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAAC                                                                                         >  SRR4302533.2017208/1‑94 (MQ=60)

     CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAAC                                                                                         <  SRR4302533.3985892/94‑1 (MQ=60)

     CTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAAC                                                                                         <  SRR4302533.4806593/94‑1 (MQ=60)

      TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑CAGGCAAAAACT                                                                                        >  SRR4302533.2191040/1‑94 (MQ=60)

      TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACT                                                                                        >  SRR4302533.5903775/1‑94 (MQ=60)

      TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACT                                                                                        <  SRR4302533.1320186/94‑1 (MQ=60)

      TTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACT                                                                                        <  SRR4302533.4721783/94‑1 (MQ=60)

        CCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTC                                                                                      >  SRR4302533.5510314/1‑94 (MQ=60)

        CCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTC                                                                                      <  SRR4302533.1417495/94‑1 (MQ=60)

         CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCT                                                                                     <  SRR4302533.6077301/94‑1 (MQ=60)

         CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCT                                                                                     >  SRR4302533.3496685/1‑94 (MQ=60)

         CGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCT                                                                                     <  SRR4302533.3959545/94‑1 (MQ=60)

          GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTC                                                                                    >  SRR4302533.424660/1‑94 (MQ=60)

          GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTC                                                                                    <  SRR4302533.3314751/94‑1 (MQ=60)

          GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTC                                                                                    <  SRR4302533.3834260/94‑1 (MQ=60)

          GGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTC                                                                                    >  SRR4302533.6288837/1‑94 (MQ=60)

           GTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCG                                                                                   >  SRR4302533.162987/1‑94 (MQ=60)

            TTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGC                                                                                  >  SRR4302533.5417388/1‑94 (MQ=60)

                GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCT                                                                              >  SRR4302533.3823694/1‑94 (MQ=60)

                GTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCT                                                                              <  SRR4302533.4195941/94‑1 (MQ=60)

                  CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGC                                                                            <  SRR4302533.1175971/94‑1 (MQ=60)

                  CACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGC                                                                            >  SRR4302533.1143121/1‑94 (MQ=60)

                   ACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCT                                                                           <  SRR4302533.6039034/94‑1 (MQ=60)

                   ACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCT                                                                           >  SRR4302533.5887926/1‑94 (MQ=60)

                   ACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCT                                                                           >  SRR4302533.2704575/1‑94 (MQ=60)

                     AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCTAGGATTCCATATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCA                                                                         >  SRR4302533.1089529/1‑94 (MQ=60)

                     AAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCA                                                                         >  SRR4302533.4527397/1‑94 (MQ=60)

                       GAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAG                                                                       >  SRR4302533.2748647/1‑94 (MQ=60)

                        AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      >  SRR4302533.3158848/1‑94 (MQ=60)

                        AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      >  SRR4302533.4845927/1‑94 (MQ=60)

                        AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      <  SRR4302533.1315390/94‑1 (MQ=60)

                        AATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGC                                                                      <  SRR4302533.3221702/94‑1 (MQ=60)

                         ATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCG                                                                     <  SRR4302533.3625626/94‑1 (MQ=60)

                          TCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGC                                                                    <  SRR4302533.1460339/94‑1 (MQ=60)

                           CGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAAGC‑AAAAACTTCTCGCCCCTGCTCAAGCGCA                                                                   >  SRR4302533.4300278/1‑94 (MQ=60)

                           CGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCA                                                                   >  SRR4302533.4288592/1‑94 (MQ=60)

                            GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302533.4400985/1‑94 (MQ=60)

                            GCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCAC                                                                  >  SRR4302533.4346497/1‑94 (MQ=60)

                             CACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACA                                                                 >  SRR4302533.1505287/1‑94 (MQ=60)

                              ACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302533.4253160/1‑94 (MQ=60)

                              ACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAA                                                                >  SRR4302533.2880403/1‑94 (MQ=60)

                               CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               <  SRR4302533.1617674/94‑1 (MQ=60)

                               CCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAAC                                                               <  SRR4302533.1271113/94‑1 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302533.4277181/1‑94 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302533.1327589/94‑1 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302533.4223820/1‑94 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302533.2640810/1‑94 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              >  SRR4302533.1510774/1‑94 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302533.537671/94‑1 (MQ=60)

                                CTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACG                                                              <  SRR4302533.3536007/94‑1 (MQ=60)

                                 TTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGT                                                             <  SRR4302533.2814805/94‑1 (MQ=60)

                                  TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            <  SRR4302533.4795552/94‑1 (MQ=60)

                                  TGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTG                                                            >  SRR4302533.2241809/1‑94 (MQ=60)

                                   GTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGC                                                           >  SRR4302533.2189674/1‑94 (MQ=60)

                                    TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          <  SRR4302533.3695117/94‑1 (MQ=60)

                                    TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          >  SRR4302533.5163171/1‑94 (MQ=60)

                                    TTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCT                                                          <  SRR4302533.3391540/94‑1 (MQ=60)

                                     TTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTG                                                         >  SRR4302533.153134/1‑94 (MQ=60)

                                       TATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTC                                                       <  SRR4302533.5122546/94‑1 (MQ=60)

                                       TATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTC                                                       <  SRR4302533.2023388/94‑1 (MQ=60)

                                         TTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAC                                                     <  SRR4302533.2377354/94‑1 (MQ=60)

                                         TTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAC                                                     <  SRR4302533.6093393/94‑1 (MQ=60)

                                          TAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACC                                                    >  SRR4302533.333434/1‑94 (MQ=60)

                                           AACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCA                                                   >  SRR4302533.4384801/1‑94 (MQ=60)

                                            ACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAG                                                  <  SRR4302533.2064771/94‑1 (MQ=60)

                                             CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 <  SRR4302533.1185385/94‑1 (MQ=60)

                                             CCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGC                                                 >  SRR4302533.4627798/1‑94 (MQ=60)

                                              CAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑ACGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCG                                                >  SRR4302533.3404599/1‑94 (MQ=60)

                                              CAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCG                                                <  SRR4302533.3391510/94‑1 (MQ=60)

                                               AGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGA                                               >  SRR4302533.1762339/1‑94 (MQ=60)

                                                GTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAA                                              >  SRR4302533.389722/1‑94 (MQ=60)

                                                 TGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAC                                             >  SRR4302533.5684000/1‑94 (MQ=60)

                                                 TGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAC                                             >  SRR4302533.4663060/1‑94 (MQ=60)

                                                      CCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTA                                        >  SRR4302533.2324780/1‑94 (MQ=60)

                                                       CCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302533.3974231/1‑94 (MQ=60)

                                                       CCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302533.5952937/1‑94 (MQ=60)

                                                       CCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAC                                       >  SRR4302533.424918/1‑94 (MQ=60)

                                                        CTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      >  SRR4302533.2396570/1‑94 (MQ=60)

                                                        CTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACG                                      <  SRR4302533.3569703/94‑1 (MQ=60)

                                                         TTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGC                                     >  SRR4302533.1019634/1‑94 (MQ=60)

                                                           CGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAA                                   <  SRR4302533.1890958/94‑1 (MQ=60)

                                                            GCTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAA                                  >  SRR4302533.3733700/1‑94 (MQ=60)

                                                             CTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302533.1414767/1‑94 (MQ=60)

                                                             CTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 <  SRR4302533.2578639/94‑1 (MQ=60)

                                                             CTTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAG                                 >  SRR4302533.5225081/1‑94 (MQ=60)

                                                              TTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGC                                >  SRR4302533.4121459/1‑94 (MQ=60)

                                                              TTCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGC                                >  SRR4302533.3965628/1‑94 (MQ=60)

                                                               TCCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCC                               <  SRR4302533.4872718/94‑1 (MQ=60)

                                                                CCCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCG                              >  SRR4302533.3701236/1‑94 (MQ=60)

                                                                 CCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGC                             <  SRR4302533.5111595/94‑1 (MQ=60)

                                                                 CCGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGC                             >  SRR4302533.1911632/1‑94 (MQ=60)

                                                                  CGGATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCT                            >  SRR4302533.5664031/1‑94 (MQ=60)

                                                                    GATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          >  SRR4302533.6026020/1‑94 (MQ=60)

                                                                    GATTCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTC                          <  SRR4302533.5665150/94‑1 (MQ=60)

                                                                       TCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCAC                       >  SRR4302533.3565061/1‑94 (MQ=60)

                                                                       TCCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACCACGCAAAGCCGCTTCCAC                       >  SRR4302533.2474164/1‑94 (MQ=60)

                                                                        CCCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACC                      >  SRR4302533.4693519/1‑94 (MQ=60)

                                                                         CCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     <  SRR4302533.2777112/94‑1 (MQ=60)

                                                                         CCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     >  SRR4302533.5123655/1‑94 (MQ=60)

                                                                         CCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     >  SRR4302533.1454604/1‑94 (MQ=60)

                                                                         CCTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCA                     >  SRR4302533.5364929/1‑94 (MQ=60)

                                                                          CTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302533.6112526/1‑94 (MQ=60)

                                                                          CTATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCAC                    >  SRR4302533.6252586/1‑94 (MQ=60)

                                                                            ATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCA                  <  SRR4302533.5796060/94‑1 (MQ=60)

                                                                            ATTGGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCA                  <  SRR4302533.1330804/94‑1 (MQ=60)

                                                                               GGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               <  SRR4302533.4194819/94‑1 (MQ=60)

                                                                               GGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               <  SRR4302533.5078276/94‑1 (MQ=60)

                                                                               GGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               >  SRR4302533.34871/1‑94 (MQ=60)

                                                                               GGACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTA               >  SRR4302533.2321387/1‑94 (MQ=60)

                                                                                GACCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTAC              <  SRR4302533.4926208/94‑1 (MQ=60)

                                                                                  CCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302533.945107/1‑94 (MQ=60)

                                                                                  CCTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAC            >  SRR4302533.347091/1‑94 (MQ=60)

                                                                                   CTGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTCCGCAAAGCCGCTTCCCGCACCAGTACACT           >  SRR4302533.1277995/1‑94 (MQ=60)

                                                                                    TGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302533.417421/1‑94 (MQ=60)

                                                                                    TGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          <  SRR4302533.3724738/94‑1 (MQ=60)

                                                                                    TGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          <  SRR4302533.3067609/94‑1 (MQ=60)

                                                                                    TGGC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCT          >  SRR4302533.1183735/1‑94 (MQ=60)

                                                                                      GC‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT        >  SRR4302533.123041/1‑94 (MQ=60)

                                                                                       C‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       <  SRR4302533.5456740/94‑1 (MQ=60)

                                                                                       C‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       <  SRR4302533.3440254/94‑1 (MQ=60)

                                                                                       C‑AAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTA       >  SRR4302533.2249532/1‑94 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     <  SRR4302533.5786919/94‑1 (MQ=60)

                                                                                          AGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACT     >  SRR4302533.6113204/1‑94 (MQ=60)

                                                                                           GC‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTA   >  SRR4302533.5578309/1‑94 (MQ=60)

                                                                                           GC‑AAAAACTCCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTA   <  SRR4302533.3325139/94‑1 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302533.5796179/94‑1 (MQ=60)

                                                                                            C‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  <  SRR4302533.2771447/94‑1 (MQ=60)

AATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGC‑AAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAG  >  NC_000913/3430362‑3430546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`