Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,109 G→A P152L (CCG→CTG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1090GA100.0% 1034.8 / NA 274P152L (CCG→CTG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (135/139);  total (135/139)

Only 100 of 276 total aligned reads displayed.

GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198
                                                                                          |                                                                                         
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         <  2:3512533/93‑1 (MQ=255)
  gTCAACGCGTACTACGTTAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       <  1:1737848/93‑1 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  1:4064076/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  2:2629445/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  2:1802480/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  2:5638873/1‑93 (MQ=255)
     aaCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTg                                                                                    <  2:3274264/93‑1 (MQ=255)
     aaCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTg                                                                                    <  2:2109651/93‑1 (MQ=255)
       cgcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGt                                                                                  >  2:1323525/1‑93 (MQ=255)
        gcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGTGTCTGGtt                                                                                 <  2:1956541/93‑1 (MQ=255)
        gcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGtt                                                                                 <  1:29076/93‑1 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                <  2:643263/93‑1 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                >  1:4772826/1‑93 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                <  1:5011563/93‑1 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              <  2:373295/93‑1 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              <  2:5914800/93‑1 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              >  1:4354864/1‑93 (MQ=255)
            actacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGGTCTGa                                                                             >  1:2694265/1‑93 (MQ=255)
             ctacGTCAAGGCTCTGAACGGTTACGCGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAc                                                                            <  2:3407508/93‑1 (MQ=255)
              tacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAcc                                                                           >  2:1027883/1‑93 (MQ=255)
              tacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAcc                                                                           >  2:2930679/1‑93 (MQ=255)
              tacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAcc                                                                           <  1:3958549/93‑1 (MQ=255)
                 gTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAt                                                                        >  2:4649060/1‑93 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAg                                                                      >  1:5335479/1‑93 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAg                                                                      >  2:5304185/1‑93 (MQ=255)
                    aaGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGa                                                                     >  2:685178/1‑93 (MQ=255)
                     aGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGaa                                                                    >  1:2037002/1‑93 (MQ=255)
                        ctctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAccc                                                                 <  1:2742953/93‑1 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             >  2:4779464/1‑93 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             >  1:661083/1‑93 (MQ=255)
                             aaCGGTTACACTTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  1:5822213/93‑1 (MQ=255)
                             aaCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  2:3189556/93‑1 (MQ=255)
                               cGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  2:317440/93‑1 (MQ=255)
                                 gTTACCCGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        <  2:803613/93‑1 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        <  1:177680/93‑1 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        <  1:158829/93‑1 (MQ=255)
                                  ttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGt                                                       >  2:3656198/1‑93 (MQ=255)
                                   tACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTg                                                      <  1:5600327/93‑1 (MQ=255)
                                    acacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGCACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTga                                                     >  2:3353565/1‑93 (MQ=255)
                                      acGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgaga                                                   <  1:4674042/93‑1 (MQ=255)
                                      acGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgaga                                                   >  2:2464540/1‑93 (MQ=255)
                                       cgttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAc                                                  >  2:4575403/1‑93 (MQ=255)
                                         ttcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACaa                                                >  1:2470164/1‑93 (MQ=255)
                                          tcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAg                                               <  1:2172660/93‑1 (MQ=255)
                                            gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGa                                             <  2:4462561/93‑1 (MQ=255)
                                            gttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGa                                             >  2:1270289/1‑93 (MQ=255)
                                             ttACCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  2:3910211/93‑1 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  2:4098252/93‑1 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            >  2:5776708/1‑93 (MQ=255)
                                             ttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAg                                            <  1:3842127/93‑1 (MQ=255)
                                              tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGt                                           <  2:1575146/93‑1 (MQ=255)
                                              tACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGt                                           >  1:362629/1‑93 (MQ=255)
                                               aCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGtt                                          >  1:2526764/1‑93 (MQ=255)
                                                cccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTa                                         >  2:2391150/1‑93 (MQ=255)
                                                 ccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        <  2:4897831/93‑1 (MQ=255)
                                                  cATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAcc                                       >  2:3792260/1‑93 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     <  1:127871/93‑1 (MQ=255)
                                                     cTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTg                                    <  1:3096364/93‑1 (MQ=255)
                                                      tGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGa                                   <  2:5210912/93‑1 (MQ=255)
                                                       gACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAg                                  >  1:3774816/1‑93 (MQ=255)
                                                        cccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 <  2:5482135/92‑1 (MQ=255)
                                                         ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTa                                >  1:3144436/1‑93 (MQ=255)
                                                          cTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAg                               >  1:1387012/1‑93 (MQ=255)
                                                          cTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAg                               <  1:262229/93‑1 (MQ=255)
                                                          cTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCCGAACGCGGTGAGACAAGGAGTTACCGTGAGTAg                               >  2:5342293/1‑93 (MQ=255)
                                                             ccATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGt                            >  1:390816/1‑93 (MQ=255)
                                                              cATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTc                           >  1:113675/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         >  2:914044/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         <  2:6002541/93‑1 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGATACCGTGAGTAGCGTCCTg                        >  1:618944/1‑93 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGATCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  1:2994452/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  2:841653/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  1:5984318/93‑1 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  2:2560439/1‑93 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAACCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  1:2409243/1‑93 (MQ=255)
                                                                    cTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGt                     <  1:903279/93‑1 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTa                    <  2:3863414/93‑1 (MQ=255)
                                                                      tGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGATAAGGAGTTACCGTGAGTAGCGTCCTGGGTAc                   <  2:5039931/93‑1 (MQ=255)
                                                                       gCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACg                  <  2:6149729/93‑1 (MQ=255)
                                                                        ccTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACgg                 >  1:3248143/1‑93 (MQ=255)
                                                                        ccTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACgg                 >  2:3413779/1‑93 (MQ=255)
                                                                         cTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGa                >  2:2234895/1‑93 (MQ=255)
                                                                         cTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGa                >  2:2321747/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               >  1:5403234/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               >  2:2998917/1‑93 (MQ=255)
                                                                           ttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAg              <  2:3714361/93‑1 (MQ=255)
                                                                            tGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGt             <  1:119881/93‑1 (MQ=255)
                                                                             gAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGtt            <  1:4654239/93‑1 (MQ=255)
                                                                             gAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGtt            >  2:777992/1‑93 (MQ=255)
                                                                             gAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGtt            >  1:2138333/1‑93 (MQ=255)
                                                                              aaCACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTc           >  2:1147605/1‑93 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  2:1766607/93‑1 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  1:3371978/93‑1 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  1:1867940/93‑1 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        <  1:4517989/93‑1 (MQ=255)
                                                                                  cTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgc       >  2:5551207/1‑93 (MQ=255)
                                                                                  cTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgc       <  1:2831634/93‑1 (MQ=255)
                                                                                     tGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCt    >  2:4337914/1‑93 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  <  2:4079054/93‑1 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  <  1:2390129/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450016‑3450202
                                                                                             |                                                                                             
TCAGCGTCAACGCGTACTACGTCAGGGCTCTGAACGGTTACACGTTCGTTACCCACCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCA                                                                                               <  SRR4302533.110624/94‑1 (MQ=60)
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCA                                                                                               >  SRR4302533.1046280/1‑94 (MQ=60)
 CAGCGTCAACGCGTATTACGTCAAGGCTCTGACCGGTTCCACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACCCTTTGCCCAG                                                                                              <  SRR4302533.3931107/94‑1 (MQ=60)
 CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAG                                                                                              <  SRR4302533.5836368/94‑1 (MQ=60)
 CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAG                                                                                              <  SRR4302533.3931104/94‑1 (MQ=60)
 CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAG                                                                                              >  SRR4302533.2747116/1‑94 (MQ=60)
   GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAG                                                                                            <  SRR4302533.1765322/94‑1 (MQ=60)
    CGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGT                                                                                           >  SRR4302533.4088813/1‑94 (MQ=60)
     GTCAACGCGTACTACGTTAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          <  SRR4302533.1748391/94‑1 (MQ=60)
     GTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          >  SRR4302533.1313125/1‑94 (MQ=60)
       CAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTG                                                                                        >  SRR4302533.44176/1‑94 (MQ=60)
        AACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGG                                                                                       <  SRR4302533.3135643/94‑1 (MQ=60)
         ACGCGTACTACGTCAAGGCTCTGATCGGTTACACGTGCGTTCCCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      <  SRR4302533.3982672/94‑1 (MQ=60)
         ACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      <  SRR4302533.3217418/94‑1 (MQ=60)
         ACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      <  SRR4302533.6228047/94‑1 (MQ=60)
         ACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      <  SRR4302533.125337/94‑1 (MQ=60)
          CGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTT                                                                                     <  SRR4302533.762242/94‑1 (MQ=60)
           GCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTC                                                                                    <  SRR4302533.29273/94‑1 (MQ=60)
           GCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTC                                                                                    >  SRR4302533.4801749/1‑94 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   <  SRR4302533.3792263/94‑1 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   >  SRR4302533.1864806/1‑94 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   <  SRR4302533.5041928/94‑1 (MQ=60)
             GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302533.4381150/1‑94 (MQ=60)
              TACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGGTCTGA                                                                                 >  SRR4302533.2710775/1‑94 (MQ=60)
               ACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAC                                                                                <  SRR4302533.4568211/94‑1 (MQ=60)
                CTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACC                                                                               <  SRR4302533.1866307/94‑1 (MQ=60)
                 TACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCG                                                                              <  SRR4302533.3982673/94‑1 (MQ=60)
                    GTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATA                                                                           >  SRR4302533.3035951/1‑94 (MQ=60)
                    GTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATA                                                                           >  SRR4302533.2399859/1‑94 (MQ=60)
                     TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          <  SRR4302533.5140629/94‑1 (MQ=60)
                     TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          >  SRR4302533.5367460/1‑94 (MQ=60)
                      CAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGA                                                                         >  SRR4302533.100304/1‑94 (MQ=60)
                      CAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGA                                                                         >  SRR4302533.409907/1‑94 (MQ=60)
                       AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302533.2049563/1‑94 (MQ=60)
                       AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302533.3154304/1‑94 (MQ=60)
                       AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        <  SRR4302533.275667/94‑1 (MQ=60)
                        AGGCTCTGAACGGTTACACGTTCGTTACTCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAC                                                                       <  SRR4302533.5391891/94‑1 (MQ=60)
                        AGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAC                                                                       <  SRR4302533.2068883/94‑1 (MQ=60)
                         GGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACC                                                                      <  SRR4302533.3483388/94‑1 (MQ=60)
                         GGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACC                                                                      <  SRR4302533.3934008/94‑1 (MQ=60)
                           CTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCG                                                                    <  SRR4302533.2759771/94‑1 (MQ=60)
                           CTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCG                                                                    <  SRR4302533.4686084/94‑1 (MQ=60)
                           CTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCG                                                                    >  SRR4302533.604584/1‑94 (MQ=60)
                            TCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGG                                                                   >  SRR4302533.2674419/1‑94 (MQ=60)
                              TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 >  SRR4302533.2035809/1‑94 (MQ=60)
                              TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 >  SRR4302533.664983/1‑94 (MQ=60)
                               GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                >  SRR4302533.1356778/1‑94 (MQ=60)
                               GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                <  SRR4302533.4730540/94‑1 (MQ=60)
                               GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                <  SRR4302533.2609202/94‑1 (MQ=60)
                                AACGGTTACACTTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               <  SRR4302533.5857382/94‑1 (MQ=60)
                                AACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               >  SRR4302533.1672847/1‑94 (MQ=60)
                                   GGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGG                                                            >  SRR4302533.3843215/1‑94 (MQ=60)
                                   GGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGG                                                            >  SRR4302533.363541/1‑94 (MQ=60)
                                    GTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGT                                                           <  SRR4302533.159841/94‑1 (MQ=60)
                                    GTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGT                                                           <  SRR4302533.178793/94‑1 (MQ=60)
                                      TACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGA                                                         <  SRR4302533.5634163/94‑1 (MQ=60)
                                       ACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAG                                                        <  SRR4302533.2163639/94‑1 (MQ=60)
                                        ctggtgctttcCCCATCTGCCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGA                                                       <  SRR4302533.2497885/83‑1 (MQ=60)
                                         ACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAC                                                      >  SRR4302533.2575699/1‑94 (MQ=60)
                                         ACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGAC                                                      <  SRR4302533.4702364/94‑1 (MQ=60)
                                           GTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAA                                                    >  SRR4302533.2485401/1‑94 (MQ=60)
                                            TTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAG                                                   >  SRR4302533.2421679/1‑94 (MQ=60)
                                            TTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAG                                                   >  SRR4302533.5652601/1‑94 (MQ=60)
                                            TTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAG                                                   <  SRR4302533.1936607/94‑1 (MQ=60)
                                             TCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGG                                                  <  SRR4302533.2186020/94‑1 (MQ=60)
                                             TCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGG                                                  <  SRR4302533.748109/94‑1 (MQ=60)
                                              CGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGA                                                 <  SRR4302533.6309838/94‑1 (MQ=60)
                                                TTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGT                                               <  SRR4302533.3865599/94‑1 (MQ=60)
                                                TTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGT                                               >  SRR4302533.364835/1‑94 (MQ=60)
                                                 TACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTT                                              >  SRR4302533.2542320/1‑94 (MQ=60)
                                                 TACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTT                                              <  SRR4302533.3875468/94‑1 (MQ=60)
                                                  ACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTA                                             <  SRR4302533.3984743/94‑1 (MQ=60)
                                                     CATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCG                                          <  SRR4302533.1154378/94‑1 (MQ=60)
                                                      ATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGT                                         <  SRR4302533.4451722/94‑1 (MQ=60)
                                                      ATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGT                                         >  SRR4302533.2478359/1‑94 (MQ=60)
                                                      ATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGT                                         >  SRR4302533.2513449/1‑94 (MQ=60)
                                                       TCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTG                                        <  SRR4302533.4873206/94‑1 (MQ=60)
                                                       TCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTG                                        <  SRR4302533.128669/94‑1 (MQ=60)
                                                        CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       <  SRR4302533.3115396/94‑1 (MQ=60)
                                                         TGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAG                                      <  SRR4302533.3297841/94‑1 (MQ=60)
                                                         TGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAG                                      >  SRR4302533.5545935/1‑94 (MQ=60)
                                                         TGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAG                                      >  SRR4302533.3797955/1‑94 (MQ=60)
                                                          GACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGT                                     >  SRR4302533.1484334/1‑94 (MQ=60)
                                                           ACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTA                                    >  SRR4302533.4772870/1‑94 (MQ=60)
                                                           ACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTA                                    >  SRR4302533.3163768/1‑94 (MQ=60)
                                                            CCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAG                                   >  SRR4302533.1395360/1‑94 (MQ=60)
                                                             CTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGC                                  <  SRR4302533.3916081/94‑1 (MQ=60)
                                                             CTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGC                                  <  SRR4302533.263819/94‑1 (MQ=60)
                                                             CTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGC                                  >  SRR4302533.3229018/1‑94 (MQ=60)
                                                              TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCG                                 <  SRR4302533.2422683/94‑1 (MQ=60)
                                                              TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGGTACCGTGAGTAGCG                                 >  SRR4302533.2789463/1‑94 (MQ=60)
                                                               GCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGT                                <  SRR4302533.566100/94‑1 (MQ=60)
                                                               GCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGT                                >  SRR4302533.393181/1‑94 (MQ=60)
                                                               GCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGT                                >  SRR4302533.3304721/1‑94 (MQ=60)
                                                                CCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTC                               >  SRR4302533.114398/1‑94 (MQ=60)
                                                                CCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTC                               <  SRR4302533.4659454/94‑1 (MQ=60)
                                                                 CATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCC                              >  SRR4302533.2873272/1‑94 (MQ=60)
                                                                  ATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCT                             <  SRR4302533.5917277/94‑1 (MQ=60)
                                                                   TTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGATACCGTGAGTAGCGTCCTG                            >  SRR4302533.622578/1‑94 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGATCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302533.3012761/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          >  SRR4302533.2391968/1‑94 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302533.6016018/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302533.6020560/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAACCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          >  SRR4302533.2424125/1‑94 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        <  SRR4302533.908576/94‑1 (MQ=60)
                                                                        TTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTAC                       <  SRR4302533.2973708/94‑1 (MQ=60)
                                                                         TGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACG                      >  SRR4302533.2514020/1‑94 (MQ=60)
                                                                          GCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGG                     >  SRR4302533.3268172/1‑94 (MQ=60)
                                                                            CTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAA                   <  SRR4302533.5002835/94‑1 (MQ=60)
                                                                            CTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAA                   >  SRR4302533.5435701/1‑94 (MQ=60)
                                                                            CTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAA                   >  SRR4302533.435080/1‑94 (MQ=60)
                                                                            CTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAA                   <  SRR4302533.385769/94‑1 (MQ=60)
                                                                              TTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGT                 >  SRR4302533.5357510/1‑94 (MQ=60)
                                                                               TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTT                <  SRR4302533.120641/94‑1 (MQ=60)
                                                                               TGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTT                >  SRR4302533.2151517/1‑94 (MQ=60)
                                                                                GAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTC               <  SRR4302533.4682442/94‑1 (MQ=60)
                                                                                 AACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCA              >  SRR4302533.5538178/1‑94 (MQ=60)
                                                                                  ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302533.3392800/94‑1 (MQ=60)
                                                                                  ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302533.1879327/94‑1 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            >  SRR4302533.1781548/1‑94 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            <  SRR4302533.2934007/94‑1 (MQ=60)
                                                                                    ACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGC           <  SRR4302533.2793885/94‑1 (MQ=60)
                                                                                    ACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGC           >  SRR4302533.2698277/1‑94 (MQ=60)
                                                                                    ACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGC           >  SRR4302533.128043/1‑94 (MQ=60)
                                                                                    ACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGC           <  SRR4302533.4545349/94‑1 (MQ=60)
                                                                                     CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          <  SRR4302533.2849056/94‑1 (MQ=60)
                                                                                      TTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGC         <  SRR4302533.2892219/94‑1 (MQ=60)
                                                                                        TGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTT       >  SRR4302533.1551120/1‑94 (MQ=60)
                                                                                        TGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTT       <  SRR4302533.2156969/94‑1 (MQ=60)
                                                                                          CCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAA     <  SRR4302533.2404889/94‑1 (MQ=60)
                                                                                           CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTCAC    >  SRR4302533.4933217/1‑94 (MQ=60)
                                                                                           CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAG    >  SRR4302533.2799680/1‑94 (MQ=60)
                                                                                           CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAC    >  SRR4302533.3465583/1‑94 (MQ=60)
                                                                                           CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAC    >  SRR4302533.4625213/1‑94 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCGAGCGCTTAACG   >  SRR4302533.3324154/1‑94 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302533.5057508/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302533.896618/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   >  SRR4302533.281374/1‑94 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   >  SRR4302533.4352139/1‑94 (MQ=60)
                                                                                             AGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  SRR4302533.2427983/1‑94 (MQ=60)
                                                                                             AGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  SRR4302533.2440716/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450016‑3450202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑