Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,844 T→C T22A (ACC→GCC)  rpsJ ← 30S ribosomal subunit protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,8440TC100.0% 854.6 / NA 263T22A (ACC→GCC) rpsJ30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/161);  total (102/161)

Only 100 of 266 total aligned reads displayed.

GAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATA  >  NC_000913/3450754‑3450933
                                                                                          |                                                                                         
gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCCGTCTCGACGATTTCCGCGGCTg                                                                                         >  1:1977075/1‑93 (MQ=255)
gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTg                                                                                         >  2:2497099/1‑93 (MQ=255)
gAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTg                                                                                         >  1:3041283/1‑93 (MQ=255)
   cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCtt                                                                                      <  2:4396427/93‑1 (MQ=255)
   cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCtt                                                                                      <  2:1596206/93‑1 (MQ=255)
   cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCtt                                                                                      <  1:5570314/93‑1 (MQ=255)
    aGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTg                                                                                     >  2:5364924/1‑93 (MQ=255)
     gTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTga                                                                                    <  1:1507035/93‑1 (MQ=255)
      tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgat                                                                                   <  2:3913060/93‑1 (MQ=255)
      tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgat                                                                                   <  1:1883952/93‑1 (MQ=255)
       gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGCCCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatc                                                                                  >  2:1404012/1‑93 (MQ=255)
       gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatc                                                                                  <  2:6249478/93‑1 (MQ=255)
         aGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcga                                                                                <  2:1342051/93‑1 (MQ=255)
         aGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcga                                                                                <  1:5425514/93‑1 (MQ=255)
          gcgcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                               <  2:810837/93‑1 (MQ=255)
          gcgcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                               <  2:3749274/93‑1 (MQ=255)
            gcTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCa                                                                             <  1:561799/93‑1 (MQ=255)
             ctctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAg                                                                            >  1:5015238/1‑93 (MQ=255)
             ctctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAg                                                                            <  1:1843016/93‑1 (MQ=255)
             ctctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAg                                                                            >  1:4422282/1‑93 (MQ=255)
              tctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGa                                                                           <  2:4344258/93‑1 (MQ=255)
              tctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGa                                                                           <  1:4891823/93‑1 (MQ=255)
              tctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGa                                                                           >  2:6099168/1‑93 (MQ=255)
               ctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCGCCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGAc                                                                          <  1:5199270/93‑1 (MQ=255)
                tttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCAGCGGCTGCTTGATCGATCAGACg                                                                         >  1:3163981/1‑93 (MQ=255)
                 ttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACga                                                                        <  1:5925637/93‑1 (MQ=255)
                     gtgtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatgat                                                                    >  1:2724945/1‑93 (MQ=255)
                      tgtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATc                                                                   <  1:2612962/93‑1 (MQ=255)
                       gtCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCa                                                                  >  1:957450/1‑93 (MQ=255)
                         cgtcAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCaaa                                                                <  1:703413/90‑1 (MQ=255)
                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCaaa                                                                <  2:1572096/93‑1 (MQ=255)
                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCaaa                                                                <  2:4582169/93‑1 (MQ=255)
                          ggCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAAc                                                               <  2:2586809/93‑1 (MQ=255)
                            cAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGc                                                             <  2:5900029/93‑1 (MQ=255)
                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCtt                                                           >  2:4713507/1‑93 (MQ=255)
                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCtt                                                           >  1:1239775/1‑93 (MQ=255)
                              gCGGGATCGAACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCtt                                                           <  1:614623/93‑1 (MQ=255)
                               cGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCttt                                                          <  2:2064889/93‑1 (MQ=255)
                                gggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTc                                                         <  1:5722564/93‑1 (MQ=255)
                                 ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                        >  1:2836540/1‑93 (MQ=255)
                                 ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                        <  2:2199966/93‑1 (MQ=255)
                                 ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                        <  1:4853277/93‑1 (MQ=255)
                                 ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                        >  1:114562/1‑93 (MQ=255)
                                 ggATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                        <  1:3758111/93‑1 (MQ=255)
                                   aTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAgg                                                      <  2:4831857/93‑1 (MQ=255)
                                    tCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCGGACGATGATCAAACGCTTTCAGGc                                                     >  1:1633312/1‑93 (MQ=255)
                                    tCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGc                                                     <  1:5470423/93‑1 (MQ=255)
                                       gACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCggt                                                  >  1:3464237/1‑92 (MQ=255)
                                        aCCACGGACCTGCGCACCAGTGCGCTTGGAAGTCTCGACGATTTCCGCGGCTGCTTGATAGATCAGACGATGATCAAACGCTTTCAGGCGGAt                                                 >  2:3335233/1‑93 (MQ=255)
                                         ccACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATa                                                <  2:1164261/93‑1 (MQ=255)
                                          cACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                               >  1:514724/1‑93 (MQ=255)
                                          cACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                               >  2:2910721/1‑93 (MQ=255)
                                          cACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                               >  2:6087226/1‑93 (MQ=255)
                                           aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACg                                              >  2:4875452/1‑93 (MQ=255)
                                            cGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACgg                                             <  2:5503806/93‑1 (MQ=255)
                                             ggACCTGCGCACCAGTGCGCGTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGa                                            <  1:4029539/93‑1 (MQ=255)
                                              gACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAt                                           <  2:1648191/93‑1 (MQ=255)
                                              gACCTGCGCACCAGCGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAt                                           <  2:4088090/93‑1 (MQ=255)
                                                 cTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCt                                        <  1:2116521/93‑1 (MQ=255)
                                                   gcgcACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCttt                                      <  2:3574842/93‑1 (MQ=255)
                                                     gcACCAGTGCTCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                    <  1:4398074/93‑1 (MQ=255)
                                                        ccAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                                 <  2:5175290/93‑1 (MQ=255)
                                                        ccAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                                 <  1:4661592/93‑1 (MQ=255)
                                                         cAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCt                                >  2:1719710/1‑93 (MQ=255)
                                                           gTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGATTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGc                              <  1:4229218/93‑1 (MQ=255)
                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                             <  2:1852979/93‑1 (MQ=255)
                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                             <  2:161506/93‑1 (MQ=255)
                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                             <  2:4976425/93‑1 (MQ=255)
                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                             <  2:2920824/93‑1 (MQ=255)
                                                             gcgcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCAt                            <  1:3964326/93‑1 (MQ=255)
                                                              cgcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                           <  1:6270527/93‑1 (MQ=255)
                                                              cgcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                           >  2:3841135/1‑93 (MQ=255)
                                                              cgcTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                           >  2:688607/1‑93 (MQ=255)
                                                                 ttGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATgaga                        >  1:2333716/1‑93 (MQ=255)
                                                                  tGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAc                       >  2:2169895/1‑93 (MQ=255)
                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAcc                      <  2:4184882/93‑1 (MQ=255)
                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAcc                      <  2:2677576/93‑1 (MQ=255)
                                                                    gCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCa                     <  1:435355/93‑1 (MQ=255)
                                                                       gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCagag                  >  2:31950/1‑93 (MQ=255)
                                                                       gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCagag                  <  2:4054709/93‑1 (MQ=255)
                                                                       gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCagag                  <  2:723865/93‑1 (MQ=255)
                                                                       gTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCagag                  >  2:2784380/1‑93 (MQ=255)
                                                                          tcGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTc               >  2:3235648/1‑93 (MQ=255)
                                                                             acgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCaa            >  2:3372154/1‑93 (MQ=255)
                                                                              cgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAat           <  2:5147181/93‑1 (MQ=255)
                                                                               gaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatt          >  1:3133854/1‑93 (MQ=255)
                                                                               gaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatt          >  1:2954684/1‑93 (MQ=255)
                                                                                aTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatta         <  1:3995044/93‑1 (MQ=255)
                                                                                 tttCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAattat        >  1:79588/1‑93 (MQ=255)
                                                                                 tttCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAattat        >  1:4968291/1‑93 (MQ=255)
                                                                                  ttCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAattatt       <  1:3642454/93‑1 (MQ=255)
                                                                                   tCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt      <  1:1093502/93‑1 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     <  1:857322/93‑1 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     <  2:23222/93‑1 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     <  1:4126681/93‑1 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     >  2:2538470/1‑93 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     >  2:3041244/1‑93 (MQ=255)
                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTCCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAtttt     <  1:6027547/93‑1 (MQ=255)
                                                                                      gcgGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTtat   >  2:2470048/1‑93 (MQ=255)
                                                                                       cgGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTtata  <  2:5490628/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATA  >  NC_000913/3450754‑3450933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACG  >  NC_000913/3450751‑3450937
                                                                                             |                                                                                             
TCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGC                                                                                               >  SRR4302533.2394545/1‑94 (MQ=60)
TCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGC                                                                                               <  SRR4302533.2930951/94‑1 (MQ=60)
 CAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCT                                                                                              >  SRR4302533.834531/1‑94 (MQ=60)
  AGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCCGTCTCGACGATTTCCGCGGCTG                                                                                             >  SRR4302533.1989184/1‑94 (MQ=60)
  AGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTG                                                                                             >  SRR4302533.5004329/1‑94 (MQ=60)
  AGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTG                                                                                             >  SRR4302533.5561370/1‑94 (MQ=60)
  AGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTG                                                                                             >  SRR4302533.3059897/1‑94 (MQ=60)
  AGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTG                                                                                             >  SRR4302533.6327228/1‑94 (MQ=60)
    AACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCT                                                                                           >  SRR4302533.3165776/1‑94 (MQ=60)
     ACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTT                                                                                          <  SRR4302533.4068068/94‑1 (MQ=60)
      CAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTG                                                                                         <  SRR4302533.5603984/94‑1 (MQ=60)
       AGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGA                                                                                        >  SRR4302533.836032/1‑94 (MQ=60)
        GTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGAT                                                                                       <  SRR4302533.1516118/94‑1 (MQ=60)
         TGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATC                                                                                      >  SRR4302533.5224750/1‑94 (MQ=60)
         TGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATC                                                                                      <  SRR4302533.1895458/94‑1 (MQ=60)
           AAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGA                                                                                    <  SRR4302533.3968051/94‑1 (MQ=60)
           AAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGA                                                                                    >  SRR4302533.89655/1‑94 (MQ=60)
           AAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGA                                                                                    >  SRR4302533.5212794/1‑94 (MQ=60)
            AGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGAT                                                                                   <  SRR4302533.6227653/94‑1 (MQ=60)
            AGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGAT                                                                                   <  SRR4302533.2378980/94‑1 (MQ=60)
            AGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGAT                                                                                   <  SRR4302533.5458189/94‑1 (MQ=60)
             GCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATC                                                                                  <  SRR4302533.5612931/94‑1 (MQ=60)
              CGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCA                                                                                 >  SRR4302533.6267598/1‑94 (MQ=60)
               GCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAG                                                                                <  SRR4302533.565131/94‑1 (MQ=60)
               GCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAG                                                                                >  SRR4302533.5045623/1‑94 (MQ=60)
               GCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAG                                                                                <  SRR4302533.1136199/94‑1 (MQ=60)
               GCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAG                                                                                >  SRR4302533.4449021/1‑94 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.4064632/94‑1 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.3149092/94‑1 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.6331387/94‑1 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.5472198/94‑1 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.1854256/94‑1 (MQ=60)
                CTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGA                                                                               <  SRR4302533.5874984/94‑1 (MQ=60)
                 TCTTTGCGTGTCGGCAGGGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGAC                                                                              <  SRR4302533.3173467/94‑1 (MQ=60)
                 TCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGAC                                                                              <  SRR4302533.4970190/94‑1 (MQ=60)
                 TCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGAC                                                                              <  SRR4302533.4921462/94‑1 (MQ=60)
                 TCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGAA                                                                              >  SRR4302533.588735/1‑94 (MQ=60)
                  CTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCGCCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACG                                                                             <  SRR4302533.5230618/94‑1 (MQ=60)
                  CTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACG                                                                             >  SRR4302533.5178222/1‑94 (MQ=60)
                  CTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCAGCGGCTGCTTGATCGATCAGACG                                                                             >  SRR4302533.3183455/1‑94 (MQ=60)
                    TTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGAT                                                                           <  SRR4302533.5961459/94‑1 (MQ=60)
                    TTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGAT                                                                           <  SRR4302533.1662537/94‑1 (MQ=60)
                    TTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGAT                                                                           <  SRR4302533.4483581/94‑1 (MQ=60)
                     TGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATG                                                                          >  SRR4302533.780417/1‑94 (MQ=60)
                     TGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATG                                                                          >  SRR4302533.1884372/1‑94 (MQ=60)
                     TGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATG                                                                          <  SRR4302533.2105178/94‑1 (MQ=60)
                      GCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGA                                                                         <  SRR4302533.4696505/94‑1 (MQ=60)
                      GCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGA                                                                         <  SRR4302533.4283239/94‑1 (MQ=60)
                       CGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGAT                                                                        >  SRR4302533.2741665/1‑94 (MQ=60)
                         TGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCA                                                                      >  SRR4302533.963079/1‑94 (MQ=60)
                         TGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCA                                                                      <  SRR4302533.2629000/94‑1 (MQ=60)
                           TCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAA                                                                    <  SRR4302533.4895019/94‑1 (MQ=60)
                           TCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAA                                                                    <  SRR4302533.5597184/94‑1 (MQ=60)
                            CGTCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAAC                                                                   <  SRR4302533.707571/94‑1 (MQ=60)
                            CGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAAC                                                                   <  SRR4302533.4419663/94‑1 (MQ=60)
                            CGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAAC                                                                   <  SRR4302533.3367503/94‑1 (MQ=60)
                             GGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACG                                                                  <  SRR4302533.5711247/94‑1 (MQ=60)
                                AGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTT                                                               >  SRR4302533.4964587/1‑94 (MQ=60)
                                AGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTT                                                               >  SRR4302533.4027958/1‑94 (MQ=60)
                                AGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTT                                                               >  SRR4302533.1247182/1‑94 (MQ=60)
                                 GCGGGATCGAACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTT                                                              <  SRR4302533.618235/94‑1 (MQ=60)
                                   GGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCA                                                            >  SRR4302533.115290/1‑94 (MQ=60)
                                   GGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCA                                                            <  SRR4302533.5757116/94‑1 (MQ=60)
                                   GGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCA                                                            <  SRR4302533.1930963/94‑1 (MQ=60)
                                   GGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCA                                                            >  SRR4302533.5006844/1‑94 (MQ=60)
                                   GGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCA                                                            >  SRR4302533.2853997/1‑94 (MQ=60)
                                    GGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAG                                                           <  SRR4302533.4882688/94‑1 (MQ=60)
                                    GGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAG                                                           <  SRR4302533.3781150/94‑1 (MQ=60)
                                      ATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCGGACGATGATCAAACGCTTTCAGGC                                                         >  SRR4302533.1643204/1‑94 (MQ=60)
                                       TCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCG                                                        <  SRR4302533.2839331/94‑1 (MQ=60)
                                       TCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCG                                                        <  SRR4302533.5503392/94‑1 (MQ=60)
                                       TCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCG                                                        <  SRR4302533.1969521/94‑1 (MQ=60)
                                        CGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGG                                                       >  SRR4302533.839519/1‑94 (MQ=60)
                                         GGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGT                                                      >  SRR4302533.3485622/1‑94 (MQ=60)
                                          GACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGAT                                                     <  SRR4302533.271543/94‑1 (MQ=60)
                                          GACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGAT                                                     <  SRR4302533.3016598/94‑1 (MQ=60)
                                           GCCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATA                                                    <  SRR4302533.2499710/94‑1 (MQ=60)
                                            CCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAC                                                   >  SRR4302533.517781/1‑94 (MQ=60)
                                            CCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAC                                                   <  SRR4302533.6015449/94‑1 (MQ=60)
                                                GGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAT                                               <  SRR4302533.4007197/94‑1 (MQ=60)
                                                GGACCTGCGCACCAGTGCGCGTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGAT                                               <  SRR4302533.4054067/94‑1 (MQ=60)
                                                  ACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTC                                             >  SRR4302533.5607306/1‑94 (MQ=60)
                                                    CTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTT                                           <  SRR4302533.2129575/94‑1 (MQ=60)
                                                     TGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTT                                          >  SRR4302533.4657550/1‑94 (MQ=60)
                                                       CGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGG                                        <  SRR4302533.2884957/94‑1 (MQ=60)
                                                       CGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGG                                        <  SRR4302533.3776147/94‑1 (MQ=60)
                                                       CGCACCAGTGCGCGTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACAATGATCAAACGCTTTCAGTCGGATACGGATTCTTTGG                                        <  SRR4302533.1586989/94‑1 (MQ=60)
                                                        GCACCAGTGCTCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGT                                       <  SRR4302533.4424629/94‑1 (MQ=60)
                                                        GCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCCGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGT                                       >  SRR4302533.486905/1‑94 (MQ=60)
                                                        GCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGT                                       <  SRR4302533.2989399/94‑1 (MQ=60)
                                                         CACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTT                                      >  SRR4302533.1215924/1‑94 (MQ=60)
                                                           CCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCT                                    <  SRR4302533.4689841/94‑1 (MQ=60)
                                                             AGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGC                                  >  SRR4302533.878463/1‑94 (MQ=60)
                                                              GTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCA                                 <  SRR4302533.4169968/94‑1 (MQ=60)
                                                              GTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGATTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCA                                 <  SRR4302533.4254786/94‑1 (MQ=60)
                                                                GCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATG                               <  SRR4302533.3988477/94‑1 (MQ=60)
                                                                GCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATG                               >  SRR4302533.4396157/1‑94 (MQ=60)
                                                                 CGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGA                              >  SRR4302533.3667514/1‑94 (MQ=60)
                                                                 CGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGA                              <  SRR4302533.6307987/94‑1 (MQ=60)
                                                                  GCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAG                             >  SRR4302533.1774393/1‑94 (MQ=60)
                                                                  GCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGAGCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAG                             >  SRR4302533.2344756/1‑94 (MQ=60)
                                                                   CTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGA                            >  SRR4302533.2348165/1‑94 (MQ=60)
                                                                    TTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAC                           <  SRR4302533.2108534/94‑1 (MQ=60)
                                                                      GGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCA                         <  SRR4302533.6139064/94‑1 (MQ=60)
                                                                      GGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCA                         >  SRR4302533.3377025/1‑94 (MQ=60)
                                                                       GCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAG                        <  SRR4302533.15016/94‑1 (MQ=60)
                                                                       GCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAG                        <  SRR4302533.437950/94‑1 (MQ=60)
                                                                        CAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGA                       <  SRR4302533.1565928/94‑1 (MQ=60)
                                                                          GTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGC                     >  SRR4302533.3863023/1‑94 (MQ=60)
                                                                              CGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCA                 <  SRR4302533.1236179/94‑1 (MQ=60)
                                                                                ACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAAT               <  SRR4302533.2648424/94‑1 (MQ=60)
                                                                                ACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATACAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCCAT               >  SRR4302533.6062273/1‑94 (MQ=60)
                                                                                 CGATTTCGGCGGCTGCTTGAAAGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCACAGCTCCAATA              >  SRR4302533.1047055/1‑94 (MQ=60)
                                                                                 CGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATT              >  SRR4302533.2972752/1‑94 (MQ=60)
                                                                                 CGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATT              >  SRR4302533.3153124/1‑94 (MQ=60)
                                                                                   ATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAT            <  SRR4302533.5416835/94‑1 (MQ=60)
                                                                                   ATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAT            >  SRR4302533.4998413/1‑94 (MQ=60)
                                                                                   ATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAT            >  SRR4302533.80096/1‑94 (MQ=60)
                                                                                   ATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAT            <  SRR4302533.4019369/94‑1 (MQ=60)
                                                                                    TTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATT           <  SRR4302533.5415359/94‑1 (MQ=60)
                                                                                     TTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCTGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTT          <  SRR4302533.3636425/94‑1 (MQ=60)
                                                                                     TTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTT          <  SRR4302533.3664851/94‑1 (MQ=60)
                                                                                      TCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTT         <  SRR4302533.1099971/94‑1 (MQ=60)
                                                                                      TCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTT         >  SRR4302533.2941045/1‑94 (MQ=60)
                                                                                       CCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTA        <  SRR4302533.4151731/94‑1 (MQ=60)
                                                                                       CCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTA        <  SRR4302533.4041760/94‑1 (MQ=60)
                                                                                       CCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTA        <  SRR4302533.862343/94‑1 (MQ=60)
                                                                                       CCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTCCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTA        <  SRR4302533.6064073/94‑1 (MQ=60)
                                                                                        CGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTAT       <  SRR4302533.4292887/94‑1 (MQ=60)
                                                                                         GCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATA      <  SRR4302533.4438020/94‑1 (MQ=60)
                                                                                            GCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAAC   <  SRR4302533.709265/94‑1 (MQ=60)
                                                                                            GCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAAC   <  SRR4302533.2063300/94‑1 (MQ=60)
                                                                                             CTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACG  <  SRR4302533.1180532/94‑1 (MQ=60)
                                                                                             |                                                                                             
TCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACG  >  NC_000913/3450751‑3450937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑