Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,456,098	A→G	Q41Q (CAA→CAG)	yheG →	YheG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,456,098	0	A	G	99.6%	845.0 / NA	262	Q41Q (CAA→CAG)	yheG	YheG
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (136/124); total (138/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

GCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456009‑3456187

  gcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCa                                                                                      <  1:1381235/93‑1 (MQ=255)

   cTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAg                                                                                     <  2:2741575/93‑1 (MQ=255)

   cTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAg                                                                                     >  2:3093458/1‑93 (MQ=255)

   cTGGCACAACGAATTGGTTAACTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAg                                                                                     >  2:3059888/1‑93 (MQ=255)

    tGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAgg                                                                                    >  1:95296/1‑93 (MQ=255)

     ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                   >  1:4607467/1‑93 (MQ=255)

     ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                   <  2:1394017/93‑1 (MQ=255)

      gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                  <  1:5887410/93‑1 (MQ=255)

      gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                  <  2:6212038/93‑1 (MQ=255)

       cacaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTg                                                                                 >  2:2458121/1‑93 (MQ=255)

       cacaACGAATTGGTTAGCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTg                                                                                 <  2:5345607/93‑1 (MQ=255)

          aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                              <  2:4882573/93‑1 (MQ=255)

          aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                              <  1:4445875/93‑1 (MQ=255)

           aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             <  2:411894/93‑1 (MQ=255)

             gAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGt                                                                           <  1:1727477/93‑1 (MQ=255)

              aaTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTg                                                                          >  1:3998178/1‑93 (MQ=255)

              aaTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTg                                                                          >  2:2473804/1‑93 (MQ=255)

               aTTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGc                                                                         <  1:3821449/93‑1 (MQ=255)

                ttGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGcc                                                                        >  1:1676756/1‑93 (MQ=255)

                   gTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGa                                                                     >  1:3152100/1‑93 (MQ=255)

                   gTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGa                                                                     <  1:1297808/93‑1 (MQ=255)

                    ttATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAc                                                                    <  1:4781895/93‑1 (MQ=255)

                    ttATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAc                                                                    <  1:4912388/93‑1 (MQ=255)

                      aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCa                                                                  >  1:1151417/1‑93 (MQ=255)

                      aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCa                                                                  <  2:3533963/93‑1 (MQ=255)

                      aTCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCa                                                                  <  2:4975534/93‑1 (MQ=255)

                       tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                 <  1:2322123/93‑1 (MQ=255)

                         tctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGa                                                               >  1:58339/1‑93 (MQ=255)

                         tctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGa                                                               <  1:5988376/93‑1 (MQ=255)

                         tctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGa                                                               <  1:5083032/93‑1 (MQ=255)

                          ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAc                                                              <  2:4143572/93‑1 (MQ=255)

                           tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             <  2:706941/93‑1 (MQ=255)

                           tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             <  1:1920060/93‑1 (MQ=255)

                            aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCa                                                            <  2:2356546/93‑1 (MQ=255)

                            aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCa                                                            >  2:2927533/1‑93 (MQ=255)

                              tCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGc                                                          >  1:2679869/1‑93 (MQ=255)

                               cAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCt                                                         <  2:2611186/93‑1 (MQ=255)

                                agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        <  2:1719598/93‑1 (MQ=255)

                                agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        >  1:5519880/1‑93 (MQ=255)

                                agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        <  2:3494008/93‑1 (MQ=255)

                                   gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGGTACTCGATATTCAGCGTCAGGTTAGCCAGTGCCAGACCATGACCAGCTTGTc                                                     >  2:1207585/1‑93 (MQ=255)

                                     gCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTcac                                                   >  1:6277324/1‑93 (MQ=255)

                                       gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                 >  2:5255891/1‑93 (MQ=255)

                                       gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                 >  2:4258063/1‑93 (MQ=255)

                                       gagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAg                                                 <  1:4664460/93‑1 (MQ=255)

                                        agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                                >  2:740920/1‑93 (MQ=255)

                                         gaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                               >  1:4348846/1‑93 (MQ=255)

                                         gaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                               <  1:5028687/93‑1 (MQ=255)

                                          attattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              <  1:348341/93‑1 (MQ=255)

                                            tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGa                                            >  2:6097052/1‑93 (MQ=255)

                                              tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                          >  1:3118169/1‑93 (MQ=255)

                                               ttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAttt                                         >  2:1514859/1‑93 (MQ=255)

                                                  tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGa                                      <  1:2253178/93‑1 (MQ=255)

                                                  tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGa                                      >  1:3745359/1‑93 (MQ=255)

                                                   ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  1:5739512/93‑1 (MQ=255)

                                                   ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  2:3715303/93‑1 (MQ=255)

                                                   ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  2:5568958/93‑1 (MQ=255)

                                                   ctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  1:6151338/93‑1 (MQ=255)

                                                    tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAg                                    >  2:1976282/1‑93 (MQ=255)

                                                     cGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGc                                   <  2:5500421/93‑1 (MQ=255)

                                                      gCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCt                                  >  1:4182946/1‑93 (MQ=255)

                                                         gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:3659343/1‑93 (MQ=255)

                                                          aTACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGc                              >  2:4032683/1‑93 (MQ=255)

                                                            aCGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTg                            >  1:6202474/1‑93 (MQ=255)

                                                             cGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg                           <  1:5414811/93‑1 (MQ=255)

                                                             cGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg                           >  1:3269458/1‑93 (MQ=255)

                                                              gCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGa                          <  2:1126012/93‑1 (MQ=255)

                                                               ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  2:4585840/93‑1 (MQ=255)

                                                               ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCCGCTTGaa                         >  1:713898/1‑93 (MQ=255)

                                                                cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAg                        >  1:5259061/1‑93 (MQ=255)

                                                                 aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGGAGc                       >  2:5921375/1‑93 (MQ=255)

                                                                 aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       <  1:5995574/93‑1 (MQ=255)

                                                                 aTTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGc                       >  2:3208365/1‑93 (MQ=255)

                                                                   tCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCgg                     >  1:1812129/1‑93 (MQ=255)

                                                                    cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                    <  2:5298943/93‑1 (MQ=255)

                                                                     gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTTAAGCTCGGCTGGGAGCggga                   >  2:3327686/1‑91 (MQ=255)

                                                                     gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTa                   <  1:1487112/93‑1 (MQ=255)

                                                                      aGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAt                  >  2:1467617/1‑93 (MQ=255)

                                                                      aGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAt                  >  2:658293/1‑93 (MQ=255)

                                                                          gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              >  2:393459/1‑93 (MQ=255)

                                                                          gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              <  1:5691025/93‑1 (MQ=255)

                                                                          gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              >  2:5982619/1‑93 (MQ=255)

                                                                           ttACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAt             <  2:874766/93‑1 (MQ=255)

                                                                            tACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATc            >  2:4490423/1‑93 (MQ=255)

                                                                            tACTCGATATTCACCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGCTTTTGAAGCTCAGCTGGGAGCGGGATTCCATc            >  2:467649/1‑93 (MQ=255)

                                                                             aCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCa           <  1:5773373/93‑1 (MQ=255)

                                                                             aCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCa           <  2:1884385/93‑1 (MQ=255)

                                                                              cTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAg          <  2:5691242/93‑1 (MQ=255)

                                                                               tCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGa         >  1:1997100/1‑93 (MQ=255)

                                                                               tCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGa         >  2:5774755/1‑93 (MQ=255)

                                                                                 gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       <  1:3059028/93‑1 (MQ=255)

                                                                                 gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       >  1:1096917/1‑93 (MQ=255)

                                                                                  atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      <  1:2849738/93‑1 (MQ=255)

                                                                                   tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc     <  2:3980432/93‑1 (MQ=255)

                                                                                   tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc     <  2:3287567/93‑1 (MQ=255)

                                                                                   tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAc     <  1:1236350/93‑1 (MQ=255)

                                                                                   tatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGATGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGAATAc     >  1:3242474/1‑93 (MQ=255)

                                                                                     ttCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCg   <  2:19859/93‑1 (MQ=255)

                                                                                      tCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCgg  <  2:3416649/93‑1 (MQ=255)

                                                                                      tCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCgg  >  2:1689212/1‑93 (MQ=255)

GCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456009‑3456187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  >  NC_000913/3456005‑3456191

TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAG                                                                                               <  SRR4302533.512264/94‑1 (MQ=60)

      GCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAG                                                                                         >  SRR4302533.3270132/1‑94 (MQ=60)

      GCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAG                                                                                         <  SRR4302533.1389551/94‑1 (MQ=60)

       CTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGG                                                                                        >  SRR4302533.95893/1‑94 (MQ=60)

        TGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGT                                                                                       >  SRR4302533.4635372/1‑94 (MQ=60)

          GCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTG                                                                                     <  SRR4302533.5922981/94‑1 (MQ=60)

            ACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGC                                                                                   <  SRR4302533.6165540/94‑1 (MQ=60)

              AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCA                                                                                 <  SRR4302533.5685375/94‑1 (MQ=60)

              AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCA                                                                                 <  SRR4302533.4472767/94‑1 (MQ=60)

               ACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAG                                                                                <  SRR4302533.439159/94‑1 (MQ=60)

                CGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGT                                                                               <  SRR4302533.1819104/94‑1 (MQ=60)

                CGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGT                                                                               <  SRR4302533.2665917/94‑1 (MQ=60)

                 GAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTG                                                                              >  SRR4302533.4022520/1‑94 (MQ=60)

                 GAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTG                                                                              <  SRR4302533.1737959/94‑1 (MQ=60)

                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302533.550789/1‑94 (MQ=60)

                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            <  SRR4302533.3844816/94‑1 (MQ=60)

                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302533.1686909/1‑94 (MQ=60)

                   ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                            >  SRR4302533.4410796/1‑94 (MQ=60)

                     TGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAG                                                                          <  SRR4302533.358440/94‑1 (MQ=60)

                      GGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGA                                                                         >  SRR4302533.3171479/1‑94 (MQ=60)

                       GTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGAC                                                                        <  SRR4302533.1305621/94‑1 (MQ=60)

                        TTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACC                                                                       <  SRR4302533.4810867/94‑1 (MQ=60)

                        TTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACC                                                                       <  SRR4302533.4942146/94‑1 (MQ=60)

                        TTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACC                                                                       >  SRR4302533.2119378/1‑94 (MQ=60)

                         TATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCA                                                                      >  SRR4302533.1697304/1‑94 (MQ=60)

                         TATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCA                                                                      >  SRR4302533.1158314/1‑94 (MQ=60)

                         TATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCA                                                                      >  SRR4302533.1217468/1‑94 (MQ=60)

                          ATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAT                                                                     <  SRR4302533.3880715/94‑1 (MQ=60)

                           TCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATG                                                                    >  SRR4302533.5660724/1‑94 (MQ=60)

                           TCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATG                                                                    <  SRR4302533.2336495/94‑1 (MQ=60)

                            CTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGA                                                                   <  SRR4302533.5725795/94‑1 (MQ=60)

                            CTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGA                                                                   >  SRR4302533.58724/1‑94 (MQ=60)

                             TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGCCCATGAC                                                                  >  SRR4302533.687998/1‑94 (MQ=60)

                             TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAC                                                                  <  SRR4302533.5113753/94‑1 (MQ=60)

                             TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAC                                                                  <  SRR4302533.6024642/94‑1 (MQ=60)

                             TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAC                                                                  <  SRR4302533.860154/94‑1 (MQ=60)

                              CTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACC                                                                 <  SRR4302533.1437495/94‑1 (MQ=60)

                               TATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCA                                                                <  SRR4302533.1931782/94‑1 (MQ=60)

                               TATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCA                                                                >  SRR4302533.4554743/1‑94 (MQ=60)

                                ATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAG                                                               >  SRR4302533.5830649/1‑94 (MQ=60)

                                ATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAG                                                               <  SRR4302533.3303239/94‑1 (MQ=60)

                                 TTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGC                                                              >  SRR4302533.2696294/1‑94 (MQ=60)

                                   CAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTT                                                            >  SRR4302533.5553210/1‑94 (MQ=60)

                                    AGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTG                                                           <  SRR4302533.3706200/94‑1 (MQ=60)

                                     GAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT                                                          >  SRR4302533.5843920/1‑94 (MQ=60)

                                     GAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT                                                          >  SRR4302533.1316428/1‑94 (MQ=60)

                                      AGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTC                                                         >  SRR4302533.1735292/1‑94 (MQ=60)

                                        GGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCAC                                                       >  SRR4302533.6314804/1‑94 (MQ=60)

                                        GGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCAC                                                       >  SRR4302533.4073498/1‑94 (MQ=60)

                                          CGATATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAG                                                     <  SRR4302533.5459938/94‑1 (MQ=60)

                                          CGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAG                                                     >  SRR4302533.4711017/1‑94 (MQ=60)

                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    <  SRR4302533.1208077/94‑1 (MQ=60)

                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    >  SRR4302533.3167005/1‑94 (MQ=60)

                                           GAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGG                                                    <  SRR4302533.4692721/94‑1 (MQ=60)

                                            AGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGC                                                   >  SRR4302533.4848348/1‑94 (MQ=60)

                                            AGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGC                                                   >  SRR4302533.4375063/1‑94 (MQ=60)

                                             GATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCT                                                  <  SRR4302533.5059135/94‑1 (MQ=60)

                                              ATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                 >  SRR4302533.174163/1‑94 (MQ=60)

                                              ATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                 <  SRR4302533.350468/94‑1 (MQ=60)

                                                TATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAT                                               >  SRR4302533.597435/1‑94 (MQ=60)

                                                TATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAT                                               >  SRR4302533.2204045/1‑94 (MQ=60)

                                                TATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAT                                               >  SRR4302533.125838/1‑94 (MQ=60)

                                                 ATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATT                                              >  SRR4302533.3137342/1‑94 (MQ=60)

                                                  TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTT                                             >  SRR4302533.1649924/1‑94 (MQ=60)

                                                  TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTT                                             >  SRR4302533.1969386/1‑94 (MQ=60)

                                                  TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTT                                             >  SRR4302533.4664069/1‑94 (MQ=60)

                                                    TATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           >  SRR4302533.280581/1‑94 (MQ=60)

                                                    TATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTG                                           >  SRR4302533.4753120/1‑94 (MQ=60)

                                                     ATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGA                                          >  SRR4302533.4028797/1‑94 (MQ=60)

                                                     ATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGA                                          >  SRR4302533.3768327/1‑94 (MQ=60)

                                                     ATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGA                                          <  SRR4302533.3265444/94‑1 (MQ=60)

                                                      TCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAA                                         <  SRR4302533.2267113/94‑1 (MQ=60)

                                                      TCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAA                                         <  SRR4302533.5793744/94‑1 (MQ=60)

                                                      TCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAA                                         <  SRR4302533.6153078/94‑1 (MQ=60)

                                                      TCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACAAGCTTGTCACAGGCTGGTTTTGAA                                         >  SRR4302533.1142695/1‑94 (MQ=60)

                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        >  SRR4302533.4485944/1‑94 (MQ=60)

                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        <  SRR4302533.5774148/94‑1 (MQ=60)

                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        <  SRR4302533.3149984/94‑1 (MQ=60)

                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        <  SRR4302533.6188360/94‑1 (MQ=60)

                                                       CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                        >  SRR4302533.5093876/1‑94 (MQ=60)

                                                        TCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCCGCTTGTCACAGGCTGATTTTGAAGC                                       <  SRR4302533.5890874/94‑1 (MQ=60)

                                                         CGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCT                                      >  SRR4302533.4208277/1‑94 (MQ=60)

                                                          GCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTC                                     >  SRR4302533.134970/1‑94 (MQ=60)

                                                           CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                    <  SRR4302533.336896/94‑1 (MQ=60)

                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   >  SRR4302533.3681841/1‑94 (MQ=60)

                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   <  SRR4302533.5789995/94‑1 (MQ=60)

                                                            CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                   <  SRR4302533.3230731/94‑1 (MQ=60)

                                                             GATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGC                                  >  SRR4302533.6232964/1‑94 (MQ=60)

                                                             GATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGC                                  >  SRR4302533.3109292/1‑94 (MQ=60)

                                                              ATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCT                                 >  SRR4302533.6312703/1‑94 (MQ=60)

                                                               TACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTG                                >  SRR4302533.6239673/1‑94 (MQ=60)

                                                                ACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGG                               >  SRR4302533.3289630/1‑94 (MQ=60)

                                                                 CGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGA                              <  SRR4302533.5447370/94‑1 (MQ=60)

                                                                 CGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGA                              <  SRR4302533.5985494/94‑1 (MQ=60)

                                                                  GCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAA                             <  SRR4302533.387913/94‑1 (MQ=60)

                                                                  GCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCCGCTTGAA                             >  SRR4302533.718113/1‑94 (MQ=60)

                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            >  SRR4302533.5290637/1‑94 (MQ=60)

                                                                   CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                            <  SRR4302533.5092014/94‑1 (MQ=60)

                                                                     ATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCG                          <  SRR4302533.6031887/94‑1 (MQ=60)

                                                                      TTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGG                         >  SRR4302533.1823199/1‑94 (MQ=60)

                                                                       TCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGT                        <  SRR4302533.1406905/94‑1 (MQ=60)

                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       <  SRR4302533.5224115/94‑1 (MQ=60)

                                                                        CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                       >  SRR4302533.733721/1‑94 (MQ=60)

                                                                         GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                      <  SRR4302533.1496083/94‑1 (MQ=60)

                                                                         GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                      <  SRR4302533.4820471/94‑1 (MQ=60)

                                                                         GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                      >  SRR4302533.2633212/1‑94 (MQ=60)

                                                                         GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                      <  SRR4302533.1674148/94‑1 (MQ=60)

                                                                          AGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATT                     >  SRR4302533.2282951/1‑94 (MQ=60)

                                                                             GGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCA                  >  SRR4302533.284047/1‑94 (MQ=60)

                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 <  SRR4302533.5725396/94‑1 (MQ=60)

                                                                              GTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCAT                 <  SRR4302533.3091675/94‑1 (MQ=60)

                                                                               TTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATC                >  SRR4302533.5380095/1‑94 (MQ=60)

                                                                                TACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCA               >  SRR4302533.4961116/1‑94 (MQ=60)

                                                                                TACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCA               >  SRR4302533.382786/1‑94 (MQ=60)

                                                                                 ACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAG              <  SRR4302533.5808241/94‑1 (MQ=60)

                                                                                 ACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAG              >  SRR4302533.1925048/1‑94 (MQ=60)

                                                                                 ACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGCATTCCCTCAG              >  SRR4302533.6215874/1‑94 (MQ=60)

                                                                                  CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGA             >  SRR4302533.6031637/1‑94 (MQ=60)

                                                                                  CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGA             >  SRR4302533.2009384/1‑94 (MQ=60)

                                                                                  CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGA             >  SRR4302533.2009383/1‑94 (MQ=60)

                                                                                   TCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAA            >  SRR4302533.3699707/1‑94 (MQ=60)

                                                                                    CGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAT           >  SRR4302533.1103414/1‑94 (MQ=60)

                                                                                    CGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAT           <  SRR4302533.4322574/94‑1 (MQ=60)

                                                                                     GATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATA          <  SRR4302533.3077799/94‑1 (MQ=60)

                                                                                      ATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAC         >  SRR4302533.5157855/1‑94 (MQ=60)

                                                                                      ATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAC         <  SRR4302533.2867271/94‑1 (MQ=60)

                                                                                      ATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGATGATTTTGAAGCTCGGCTGGAAGCGGGATTCCATCAGAATAC         >  SRR4302533.3262458/1‑94 (MQ=60)

                                                                                       TATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACC        <  SRR4302533.1243738/94‑1 (MQ=60)

                                                                                        ATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCG       >  SRR4302533.695907/1‑94 (MQ=60)

                                                                                         TTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG      >  SRR4302533.298428/1‑94 (MQ=60)

                                                                                             GCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302533.6131219/94‑1 (MQ=60)

                                                                                             GCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302533.194447/94‑1 (MQ=60)

                                                                                             GCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302533.4197156/94‑1 (MQ=60)

                                                                                             GCGTCAGGTGGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  <  SRR4302533.688950/94‑1 (MQ=60)

TTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAG  >  NC_000913/3456005‑3456191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`