Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,575,446 +G coding (912/942 nt) gntR ← GntR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,575,4451.G99.6% 800.1 / ‑4.0 251coding (913/942 nt)gntRGntR
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (145/105);  total (145/106)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 276 total aligned reads displayed.

ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑G‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC  >  NC_000913/3575353‑3575532
                                                                                             |                                                                                        
aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                           <  2:3091696/93‑1 (MQ=255)
aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                           <  2:3082404/93‑1 (MQ=255)
aTTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca                                                                                           >  1:711634/1‑93 (MQ=255)
 tttGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑g                                                                                         >  1:6205755/1‑93 (MQ=255)
  ttGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑gg                                                                                        <  2:879053/93‑2 (MQ=255)
    gtgGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑ggcg                                                                                      <  1:2699536/93‑4 (MQ=255)
     tgGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCcagca‑ggcgt                                                                                     >  1:5944595/1‑89 (MQ=255)
        gCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCa                                                                                  >  2:5701646/1‑93 (MQ=255)
         cTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAg                                                                                 >  2:1680599/1‑93 (MQ=255)
          ttAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAgc                                                                                >  2:2082604/1‑93 (MQ=255)
          ttAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GTCGTTCAgc                                                                                <  2:6125786/93‑1 (MQ=255)
            aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAgcgc                                                                              >  2:5268638/1‑93 (MQ=255)
            aaaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAgcgc                                                                              >  2:5171629/1‑93 (MQ=255)
             aaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGcc                                                                             >  2:4871605/1‑93 (MQ=255)
             aaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGcc                                                                             <  1:5961551/93‑1 (MQ=255)
             aaTAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGcc                                                                             >  1:1383661/1‑93 (MQ=255)
               tAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGccca                                                                           >  1:4033247/1‑91 (MQ=255)
                aGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAAt                                                                          <  1:2812456/93‑1 (MQ=255)
                aGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAAt                                                                          >  1:396013/1‑93 (MQ=255)
                 gATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATa                                                                         >  1:5638386/1‑93 (MQ=255)
                    ccGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTg                                                                      >  1:2969634/1‑93 (MQ=255)
                    ccGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTg                                                                      <  2:4936526/93‑1 (MQ=255)
                     cGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGc                                                                     >  1:3111097/1‑93 (MQ=255)
                     cGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGc                                                                     >  2:1181168/1‑93 (MQ=255)
                       cccGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGccc                                                                   >  1:5023492/1‑93 (MQ=255)
                       cccGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGccc                                                                   >  2:3032930/1‑93 (MQ=255)
                        ccGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCa                                                                  >  2:3966306/1‑93 (MQ=255)
                         cGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCAt                                                                 >  1:2480999/1‑93 (MQ=255)
                          ggTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATc                                                                <  2:3599530/93‑1 (MQ=255)
                          ggTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATc                                                                <  2:41344/93‑1 (MQ=255)
                          ggTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATc                                                                >  2:5087883/1‑93 (MQ=255)
                             gACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGc                                                             <  2:3182988/93‑1 (MQ=255)
                                aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCa                                                          <  1:2342026/93‑1 (MQ=255)
                                aaGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAAAACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCa                                                          >  2:1849249/1‑93 (MQ=255)
                                 aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                         <  1:101334/93‑1 (MQ=255)
                                 aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                         >  2:3695870/1‑93 (MQ=255)
                                 aGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCAc                                                         >  1:254710/1‑93 (MQ=255)
                                    tGAAACCTAAATCTAACATAATCGGAGTCACAGATTCTCCACTTATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCAcgcg                                                      >  2:6296469/1‑93 (MQ=255)
                                     gAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACgcgg                                                     >  2:5343335/1‑93 (MQ=255)
                                         ccgaaaTCAAACATGTTCGTGGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCTCCAAGACTGCCCATCCGCTCACGCGGCGTc                                                 <  1:222535/90‑1 (MQ=255)
                                         ccTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTc                                                 >  1:3345344/1‑93 (MQ=255)
                                          cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                <  2:5072686/93‑1 (MQ=255)
                                          cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                <  2:3842082/93‑1 (MQ=255)
                                          cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                >  1:4060980/1‑93 (MQ=255)
                                          cTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCa                                                <  1:4137211/93‑1 (MQ=255)
                                           tAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAg                                               >  1:815552/1‑93 (MQ=255)
                                              aTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCAc                                            >  2:4685861/1‑93 (MQ=255)
                                              aTCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCAc                                            >  2:2765990/1‑93 (MQ=255)
                                               tCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACg                                           >  2:2482468/1‑93 (MQ=255)
                                                 tAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                         >  2:3144149/1‑93 (MQ=255)
                                                 tAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                         >  2:1621933/1‑93 (MQ=255)
                                                 tAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCt                                         <  1:5827893/93‑1 (MQ=255)
                                                  aaCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                        >  1:450518/1‑93 (MQ=255)
                                                  aaCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                        >  1:1285244/1‑93 (MQ=255)
                                                  aaCATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                        >  1:1825075/1‑93 (MQ=255)
                                                  aaCATTTTCGGGGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTc                                        <  1:4383216/93‑1 (MQ=255)
                                                    cATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGc                                      <  1:623821/93‑1 (MQ=255)
                                                     aTTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCa                                     >  1:3245268/1‑93 (MQ=255)
                                                      ttttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCaa                                    <  1:5571617/93‑1 (MQ=255)
                                                       tttCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAg                                   >  1:4288998/1‑93 (MQ=255)
                                                         tCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTc                                 >  1:3814006/1‑93 (MQ=255)
                                                         tCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTc                                 >  2:5461034/1‑93 (MQ=255)
                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               >  1:528007/1‑93 (MQ=255)
                                                           ggTGTCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               >  2:422207/1‑93 (MQ=255)
                                                           cgtgtCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGt                               <  1:1448680/92‑1 (MQ=255)
                                                            gtgtCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTg                              >  2:2367739/1‑93 (MQ=255)
                                                            gtgtCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTg                              <  2:6207607/93‑1 (MQ=255)
                                                               tCACAGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCt                           >  1:5122259/1‑93 (MQ=255)
                                                                 acaGATTCGCCACGAATACGCGCCAGCAGG‑GGTTAAGCGCCATAATTGCCATTCGGCTCACGCGGGGCCAGAACGCCCGCAAGCCGTGGcccc                         >  2:42670/1‑93 (MQ=255)
                                                                 acaGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTcc                         >  2:4187695/1‑93 (MQ=255)
                                                                  caGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCa                        >  1:2886280/1‑93 (MQ=255)
                                                                  caGATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCa                        <  2:3901489/93‑1 (MQ=255)
                                                                    gATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATc                      >  1:918651/1‑93 (MQ=255)
                                                                    gATTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATc                      >  2:6033309/1‑93 (MQ=255)
                                                                     aTTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                     <  2:136453/93‑1 (MQ=255)
                                                                     aTTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                     >  2:3513910/1‑93 (MQ=255)
                                                                     aTTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                     <  1:551471/93‑1 (MQ=255)
                                                                     aTTCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCa                     >  2:2857121/1‑93 (MQ=255)
                                                                      ttCGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCAc                    >  1:1087320/1‑93 (MQ=255)
                                                                        cGCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCt                  <  2:4316340/93‑1 (MQ=255)
                                                                         gCCACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTg                 >  2:5848548/1‑93 (MQ=255)
                                                                          ccACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                >  2:1887212/1‑93 (MQ=255)
                                                                          ccACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                >  2:813633/1‑93 (MQ=255)
                                                                          ccACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGa                >  1:3038456/1‑93 (MQ=255)
                                                                           cACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAc               >  2:4706082/1‑93 (MQ=255)
                                                                           cACGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAc               <  1:374248/93‑1 (MQ=255)
                                                                            aCGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAcc              >  2:1080807/1‑93 (MQ=255)
                                                                            aCGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAcc              >  1:5371347/1‑93 (MQ=255)
                                                                            aCGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAcc              >  1:4830738/1‑93 (MQ=255)
                                                                             cGAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCa             >  2:1520815/1‑93 (MQ=255)
                                                                              gAATACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCaa            >  2:5915357/1‑93 (MQ=255)
                                                                                aTACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATg          >  1:3585579/1‑93 (MQ=255)
                                                                                 tACGCGCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGt         <  1:1605512/93‑1 (MQ=255)
                                                                                   cgcgCCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCa       <  2:3248882/93‑1 (MQ=255)
                                                                                   cgcgCCAGCAGG‑CGTTCAGCGCCAACACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCa       >  2:5760063/1‑93 (MQ=255)
                                                                                     cgcCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATg     >  2:4761355/1‑93 (MQ=255)
                                                                                      gcCAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGa    <  1:4520867/93‑1 (MQ=255)
                                                                                       ccAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAc   >  2:5521761/1‑93 (MQ=255)
                                                                                       ccAGCAGG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAc   >  1:2327655/1‑93 (MQ=255)
                                                                                        cagcagG‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAcc  >  1:4648223/1‑93 (MQ=255)
                                                                                             |                                                                                        
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑G‑CGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC  >  NC_000913/3575353‑3575532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAAA  >  NC_000913/3575353‑3575539
                                                                                              |                                                                                             
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑G                                                                                               >  SRR4302533.6242973/1‑94 (MQ=60)
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑G                                                                                               <  SRR4302533.4359050/94‑1 (MQ=60)
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑G                                                                                               <  SRR4302533.4831354/94‑1 (MQ=60)
    TTGGGCTTAATTAGATCCGCCCGGTGACAAGGTGAAACTTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGT                                                                                            <  SRR4302533.2461701/94‑1 (MQ=60)
    GTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGT                                                                                            >  SRR4302533.5980545/1‑94 (MQ=60)
    GTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGT                                                                                            <  SRR4302533.2716096/94‑1 (MQ=60)
       GGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCA                                                                                         <  SRR4302533.3877680/94‑1 (MQ=60)
       GGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCA                                                                                         <  SRR4302533.6247940/94‑1 (MQ=60)
       GGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCA                                                                                         <  SRR4302533.3555558/94‑1 (MQ=60)
       GGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCA                                                                                         <  SRR4302533.1374004/94‑1 (MQ=60)
        GCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAG                                                                                        <  SRR4302533.4115570/94‑1 (MQ=60)
         CTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGC                                                                                       >  SRR4302533.3811680/1‑94 (MQ=60)
         CTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGC                                                                                       >  SRR4302533.268403/1‑94 (MQ=60)
          TTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCG                                                                                      >  SRR4302533.453931/1‑94 (MQ=60)
            AAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCC                                                                                    >  SRR4302533.1418169/1‑94 (MQ=60)
            AAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCC                                                                                    >  SRR4302533.5320480/1‑94 (MQ=60)
            AAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCC                                                                                    >  SRR4302533.1391986/1‑94 (MQ=60)
             AATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCA                                                                                   <  SRR4302533.5997618/94‑1 (MQ=60)
              ATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCCA                                                                                  >  SRR4302533.4057798/1‑94 (MQ=60)
               TAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAAT                                                                                 >  SRR4302533.398412/1‑94 (MQ=60)
                AGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATA                                                                                >  SRR4302533.5672476/1‑94 (MQ=60)
                AGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATA                                                                                <  SRR4302533.2829767/94‑1 (MQ=60)
                AGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATA                                                                                <  SRR4302533.225826/94‑1 (MQ=60)
                 GATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAC                                                                               <  SRR4302533.5624686/94‑1 (MQ=60)
                 GATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATAC                                                                               >  SRR4302533.3339569/1‑94 (MQ=60)
                  ATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACT                                                                              >  SRR4302533.5069717/1‑94 (MQ=60)
                  ATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACT                                                                              <  SRR4302533.2121907/94‑1 (MQ=60)
                   TCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTG                                                                             >  SRR4302533.2343903/1‑94 (MQ=60)
                   TCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTG                                                                             >  SRR4302533.2987790/1‑94 (MQ=60)
                    CCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGC                                                                            >  SRR4302533.3130222/1‑94 (MQ=60)
                      GCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCC                                                                          >  SRR4302533.5053915/1‑94 (MQ=60)
                        CCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCAT                                                                        >  SRR4302533.2496300/1‑94 (MQ=60)
                        CCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCAT                                                                        <  SRR4302533.3211265/94‑1 (MQ=60)
                         CGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATC                                                                       >  SRR4302533.2520860/1‑94 (MQ=60)
                          GGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCC                                                                      <  SRR4302533.911999/94‑1 (MQ=60)
                           GTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCG                                                                     >  SRR4302533.3756135/1‑94 (MQ=60)
                             GACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCT                                                                   >  SRR4302533.5981026/1‑94 (MQ=60)
                             GACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCT                                                                   <  SRR4302533.2419859/94‑1 (MQ=60)
                              ACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTC                                                                  <  SRR4302533.2959719/94‑1 (MQ=60)
                               CAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCA                                                                 >  SRR4302533.2503509/1‑94 (MQ=60)
                                AAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAC                                                                >  SRR4302533.256271/1‑94 (MQ=60)
                                AAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCAC                                                                <  SRR4302533.2356526/94‑1 (MQ=60)
                                 AGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACG                                                               <  SRR4302533.101963/94‑1 (MQ=60)
                                 AGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACG                                                               >  SRR4302533.678952/1‑94 (MQ=60)
                                  GGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGC                                                              >  SRR4302533.1677662/1‑94 (MQ=60)
                                    TGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGG                                                            >  SRR4302533.1002598/1‑94 (MQ=60)
                                     GAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGC                                                           <  SRR4302533.1768492/94‑1 (MQ=60)
                                     GAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGC                                                           >  SRR4302533.549407/1‑94 (MQ=60)
                                        ACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTC                                                        >  SRR4302533.6185808/1‑94 (MQ=60)
                                        ACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTC                                                        >  SRR4302533.3366012/1‑94 (MQ=60)
                                         CCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCA                                                       >  SRR4302533.4085701/1‑94 (MQ=60)
                                         CCGAAATCAAACATGTTCGTGGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCTCCAAGACTGCCCATCCGCTCACGCGGCGTCA                                                       <  SRR4302533.223926/94‑1 (MQ=60)
                                          CTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAG                                                      <  SRR4302533.4162315/94‑1 (MQ=60)
                                          CTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAG                                                      >  SRR4302533.820332/1‑94 (MQ=60)
                                           TAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGC                                                     >  SRR4302533.4051568/1‑94 (MQ=60)
                                            AAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCA                                                    <  SRR4302533.4484408/94‑1 (MQ=60)
                                            AAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCA                                                    >  SRR4302533.70977/1‑94 (MQ=60)
                                            AAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCA                                                    >  SRR4302533.4319150/1‑94 (MQ=60)
                                             AATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCAC                                                   <  SRR4302533.3826264/94‑1 (MQ=60)
                                             AATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCAC                                                   <  SRR4302533.5891104/94‑1 (MQ=60)
                                               TCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGC                                                 >  SRR4302533.6260829/1‑94 (MQ=60)
                                                CTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCT                                                >  SRR4302533.5946281/1‑94 (MQ=60)
                                                CTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCT                                                >  SRR4302533.4748215/1‑94 (MQ=60)
                                                CTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCT                                                <  SRR4302533.5346808/94‑1 (MQ=60)
                                                CTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCT                                                >  SRR4302533.1988176/1‑94 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               >  SRR4302533.453215/1‑94 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               >  SRR4302533.3089236/1‑94 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               >  SRR4302533.1292974/1‑94 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               >  SRR4302533.1385902/1‑94 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               <  SRR4302533.5863108/94‑1 (MQ=60)
                                                 TAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTC                                               >  SRR4302533.1836224/1‑94 (MQ=60)
                                                  AACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCG                                              <  SRR4302533.139521/94‑1 (MQ=60)
                                                  AACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCG                                              <  SRR4302533.2683989/94‑1 (MQ=60)
                                                  AACATTTTCGGGGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCG                                              <  SRR4302533.4409678/94‑1 (MQ=60)
                                                    CATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCA                                            >  SRR4302533.103661/1‑94 (MQ=60)
                                                    CATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCA                                            >  SRR4302533.3265273/1‑94 (MQ=60)
                                                    CATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCA                                            <  SRR4302533.627483/94‑1 (MQ=60)
                                                     ATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAA                                           <  SRR4302533.4898361/94‑1 (MQ=60)
                                                     ATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAA                                           >  SRR4302533.4884730/1‑94 (MQ=60)
                                                     ATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAA                                           >  SRR4302533.321744/1‑94 (MQ=60)
                                                      TTTTCGGTGTCGCAGATTCGCCACGAATCCGCGCCAGCAGGCGTTCAGCGCCAATCCTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAG                                          <  SRR4302533.5572848/94‑1 (MQ=60)
                                                      TTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAG                                          <  SRR4302533.5605290/94‑1 (MQ=60)
                                                      TTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAG                                          >  SRR4302533.4314788/1‑94 (MQ=60)
                                                        TTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTC                                        >  SRR4302533.3837332/1‑94 (MQ=60)
                                                         TCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCG                                       <  SRR4302533.3989857/94‑1 (MQ=60)
                                                         TCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCG                                       <  SRR4302533.3933316/94‑1 (MQ=60)
                                                          CGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGT                                      >  SRR4302533.531149/1‑94 (MQ=60)
                                                           GGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTG                                     <  SRR4302533.4516937/94‑1 (MQ=60)
                                                           CGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTG                                     <  SRR4302533.1457420/94‑1 (MQ=60)
                                                            GTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGG                                    <  SRR4302533.781003/94‑1 (MQ=60)
                                                              GTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCT                                  >  SRR4302533.3041131/1‑94 (MQ=60)
                                                              GTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCT                                  >  SRR4302533.5153206/1‑94 (MQ=60)
                                                                 ACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCA                               >  SRR4302533.2295742/1‑94 (MQ=60)
                                                                 ACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCA                               >  SRR4302533.2904029/1‑94 (MQ=60)
                                                                 ACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCA                               >  SRR4302533.2904030/1‑94 (MQ=60)
                                                                 ACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCA                               <  SRR4302533.4068521/94‑1 (MQ=60)
                                                                   AGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATC                             >  SRR4302533.924049/1‑94 (MQ=60)
                                                                    GATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGatacagcagat                            >  SRR4302533.422160/1‑83 (MQ=60)
                                                                     ATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCAC                           >  SRR4302533.1093726/1‑94 (MQ=60)
                                                                     ATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCAC                           <  SRR4302533.554740/94‑1 (MQ=60)
                                                                        CGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTG                        >  SRR4302533.2808384/1‑94 (MQ=60)
                                                                         GCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGA                       >  SRR4302533.1553588/1‑94 (MQ=60)
                                                                         GCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGA                       >  SRR4302533.6142669/1‑94 (MQ=60)
                                                                         GCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGA                       >  SRR4302533.3335254/1‑94 (MQ=60)
                                                                         GCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGA                       >  SRR4302533.3057049/1‑94 (MQ=60)
                                                                          CCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAC                      >  SRR4302533.3577327/1‑94 (MQ=60)
                                                                          CCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGAC                      <  SRR4302533.5860383/94‑1 (MQ=60)
                                                                           CACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACC                     <  SRR4302533.376509/94‑1 (MQ=60)
                                                                           CACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACC                     >  SRR4302533.5403618/1‑94 (MQ=60)
                                                                           CACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACC                     >  SRR4302533.4860016/1‑94 (MQ=60)
                                                                            ACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCA                    <  SRR4302533.5266477/94‑1 (MQ=60)
                                                                              GAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAAT                  <  SRR4302533.4635924/94‑1 (MQ=60)
                                                                              GAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAAT                  >  SRR4302533.4586274/1‑94 (MQ=60)
                                                                              GAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAAT                  >  SRR4302533.5220880/1‑94 (MQ=60)
                                                                               AATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATG                 >  SRR4302533.3607662/1‑94 (MQ=60)
                                                                               AATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATG                 <  SRR4302533.4081961/94‑1 (MQ=60)
                                                                                 TACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTC               <  SRR4302533.1615231/94‑1 (MQ=60)
                                                                                   CGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCAT             <  SRR4302533.564675/94‑1 (MQ=60)
                                                                                   CGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCAT             <  SRR4302533.4238262/94‑1 (MQ=60)
                                                                                   CGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCAT             <  SRR4302533.289491/94‑1 (MQ=60)
                                                                                    GCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATG            <  SRR4302533.305154/94‑1 (MQ=60)
                                                                                    GCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATG            <  SRR4302533.1200123/94‑1 (MQ=60)
                                                                                      GCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAC          <  SRR4302533.92233/94‑1 (MQ=60)
                                                                                      GCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAC          <  SRR4302533.1100985/94‑1 (MQ=60)
                                                                                      GCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAC          <  SRR4302533.4548241/94‑1 (MQ=60)
                                                                                      GCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGAC          >  SRR4302533.2342063/1‑94 (MQ=60)
                                                                                       CCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC         >  SRR4302533.4676387/1‑94 (MQ=60)
                                                                                       CCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC         >  SRR4302533.5421009/1‑94 (MQ=60)
                                                                                       CCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACC         <  SRR4302533.5831629/94‑1 (MQ=60)
                                                                                        CAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCG        <  SRR4302533.2851335/94‑1 (MQ=60)
                                                                                         AGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGG       >  SRR4302533.5857828/1‑94 (MQ=60)
                                                                                           cagGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGG     <  SRR4302533.2543927/91‑1 (MQ=60)
                                                                                            agGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGA    >  SRR4302533.610731/3‑94 (MQ=60)
                                                                                            agGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGA    >  SRR4302533.4738413/3‑94 (MQ=60)
                                                                                            G‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAA   >  SRR4302533.2428189/1‑94 (MQ=60)
                                                                                            G‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAA   <  SRR4302533.4629900/94‑1 (MQ=60)
                                                                                            G‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAA   >  SRR4302533.3400169/1‑94 (MQ=60)
                                                                                            G‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAA   >  SRR4302533.2866576/1‑94 (MQ=60)
                                                                                              GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAAA  >  SRR4302533.750226/1‑94 (MQ=60)
                                                                                              |                                                                                             
ATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCA‑GCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAAA  >  NC_000913/3575353‑3575539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑