Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,897	A→G	Y354H (TAT→CAT)	ugpB ←	UgpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,897	0	A	G	100.0%	820.7 / NA	251	Y354H (TAT→CAT)	ugpB	UgpB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (127/124); total (127/124)

Only 100 of 261 total aligned reads displayed.

CGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCA  >  NC_000913/3588808‑3588986

cggcaacggcGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATgcgc                                                                                        <  2:4303794/93‑1 (MQ=255)

   caacggcGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCgctgc                                                                                     <  1:4598799/93‑1 (MQ=255)

   caacggcGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCgctgc                                                                                     <  2:5987250/93‑1 (MQ=255)

      cggcggCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCttt                                                                                  <  1:5886602/93‑1 (MQ=255)

       ggcggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTg                                                                                 >  1:1434411/1‑93 (MQ=255)

       ggcggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTg                                                                                 >  2:3905962/1‑93 (MQ=255)

       ggcggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTg                                                                                 <  2:2186193/93‑1 (MQ=255)

         cggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGt                                                                               <  1:5487555/93‑1 (MQ=255)

          ggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTg                                                                              >  2:2589215/1‑93 (MQ=255)

          ggcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGCTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTg                                                                              <  2:740036/93‑1 (MQ=255)

           gcTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGa                                                                             >  1:1924544/1‑93 (MQ=255)

            cTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGAt                                                                            <  2:1882556/93‑1 (MQ=255)

               aTTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTgg                                                                         >  1:408348/1‑93 (MQ=255)

                ttCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTggg                                                                        >  1:5932233/1‑92 (MQ=255)

                 tCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCa                                                                       <  2:5166679/93‑1 (MQ=255)

                 tCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCa                                                                       >  1:6017104/1‑93 (MQ=255)

                  cAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAg                                                                      <  2:5764531/93‑1 (MQ=255)

                  cAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAg                                                                      >  1:425770/1‑93 (MQ=255)

                   aGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGa                                                                     <  2:915540/93‑1 (MQ=255)

                    gCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAt                                                                    <  1:2399110/93‑1 (MQ=255)

                      aTCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGTTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATaa                                                                  <  2:214509/93‑1 (MQ=255)

                      aTCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATaa                                                                  <  2:1034322/93‑1 (MQ=255)

                      aTCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATaa                                                                  <  1:4952667/93‑1 (MQ=255)

                       tCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAc                                                                 <  1:3842378/93‑1 (MQ=255)

                       tCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAc                                                                 >  1:4399588/1‑93 (MQ=255)

                        cTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAcc                                                                <  2:1191526/93‑1 (MQ=255)

                         tGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCg                                                               <  1:3629643/93‑1 (MQ=255)

                         tGACGCGTCGCGGTATCCGCCCCTGGGGTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCg                                                               >  1:3944009/1‑93 (MQ=255)

                          gACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCgg                                                              >  2:1599019/1‑93 (MQ=255)

                            cgcgTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGtt                                                            <  2:3442998/93‑1 (MQ=255)

                            cgcgTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGtt                                                            <  1:5297186/93‑1 (MQ=255)

                               gTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGttttt                                                         >  2:5259650/1‑92 (MQ=255)

                               gTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTc                                                         <  2:4135526/93‑1 (MQ=255)

                               gTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTc                                                         <  1:1090681/93‑1 (MQ=255)

                               gTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTc                                                         <  1:4152875/93‑1 (MQ=255)

                                 cgcgGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTg                                                       <  1:2982837/93‑1 (MQ=255)

                                  gcgGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGa                                                      <  2:1785813/93‑1 (MQ=255)

                                   cgGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGAt                                                     <  2:4999467/93‑1 (MQ=255)

                                    ggTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATg                                                    <  2:5465072/93‑1 (MQ=255)

                                     gTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGc                                                   <  2:4747839/93‑1 (MQ=255)

                                       aTCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCa                                                 >  2:2725699/1‑93 (MQ=255)

                                        tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCAc                                                >  1:222759/1‑93 (MQ=255)

                                        tCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCAc                                                >  2:2383140/1‑93 (MQ=255)

                                         ccGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACt                                               <  2:619390/93‑1 (MQ=255)

                                          cGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTc                                              <  2:1345594/93‑1 (MQ=255)

                                          cGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTc                                              <  2:4552090/93‑1 (MQ=255)

                                           gCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCg                                             >  2:4814887/1‑93 (MQ=255)

                                             cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGc                                           <  2:306583/93‑1 (MQ=255)

                                             cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGc                                           >  1:5690005/1‑93 (MQ=255)

                                             cccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGc                                           <  1:1825490/93‑1 (MQ=255)

                                              ccTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCa                                          >  1:3469385/1‑93 (MQ=255)

                                               cTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAg                                         >  1:613274/1‑93 (MQ=255)

                                               cTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAg                                         >  1:1782239/1‑93 (MQ=255)

                                               cTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAg                                         >  1:3874905/1‑93 (MQ=255)

                                                 gggTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTTGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCg                                       >  1:630423/1‑93 (MQ=255)

                                                 gggTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCg                                       <  1:2630047/93‑1 (MQ=255)

                                                 gggTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCg                                       >  2:3171374/1‑93 (MQ=255)

                                                   gTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGtt                                     >  2:1522934/1‑93 (MQ=255)

                                                   gTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGtt                                     >  1:719207/1‑93 (MQ=255)

                                                    tttttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGttt                                    >  1:151285/1‑93 (MQ=255)

                                                    tttttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAAAACCGGTTTTCTGATGCCACTCGGCAGCGttt                                    >  1:6083182/1‑93 (MQ=255)

                                                     ttttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGtttt                                   <  1:3598576/93‑1 (MQ=255)

                                                     ttttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGtttt                                   >  1:6204404/1‑93 (MQ=255)

                                                       ttCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCt                                 <  2:4421277/93‑1 (MQ=255)

                                                        tctcATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTg                                >  1:138735/1‑93 (MQ=255)

                                                        tctcATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTg                                <  1:335476/93‑1 (MQ=255)

                                                         ctcATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTgg                               >  2:3117956/1‑93 (MQ=255)

                                                          tcATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGc                              >  1:3771909/1‑93 (MQ=255)

                                                           cATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCt                             <  1:4010930/93‑1 (MQ=255)

                                                            aTAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCtt                            >  2:3619169/1‑93 (MQ=255)

                                                            aTAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCtt                            <  2:4006046/93‑1 (MQ=255)

                                                             tAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTc                           <  2:2907404/93‑1 (MQ=255)

                                                               aaaGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTcgc                         <  2:1949316/93‑1 (MQ=255)

                                                                aaGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTcgcg                        <  1:748733/93‑1 (MQ=255)

                                                                aaGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTcgcg                        >  2:5032003/1‑93 (MQ=255)

                                                                 aGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGa                       >  1:3374518/1‑93 (MQ=255)

                                                                 aGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGa                       <  1:4189082/93‑1 (MQ=255)

                                                                  gCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAg                      <  2:422509/93‑1 (MQ=255)

                                                                   cccTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAgg                     <  1:2456238/93‑1 (MQ=255)

                                                                    ccTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGa                    >  1:51632/1‑93 (MQ=255)

                                                                    ccTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGa                    >  1:2352299/1‑93 (MQ=255)

                                                                     cTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGaa                   >  2:6268191/1‑93 (MQ=255)

                                                                     cTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGaa                   >  1:1036612/1‑93 (MQ=255)

                                                                      tGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGaaa                  >  1:4524891/1‑93 (MQ=255)

                                                                      tGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGaaa                  <  2:5846185/93‑1 (MQ=255)

                                                                      tGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTCTTCTGGCTTCGCGAGGaaa                  >  1:881143/1‑93 (MQ=255)

                                                                       gCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAAt                 <  2:4692817/93‑1 (MQ=255)

                                                                       gCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAAt                 <  2:4506990/93‑1 (MQ=255)

                                                                        cTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATc                >  1:4314391/1‑93 (MQ=255)

                                                                          cACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGa              <  1:977053/93‑1 (MQ=255)

                                                                          cACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGa              <  2:1272078/93‑1 (MQ=255)

                                                                           aCGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAg             <  2:2973000/93‑1 (MQ=255)

                                                                           aCGGGTCAGGTCATGCGCTGCTTGGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAg             <  1:2565135/93‑1 (MQ=255)

                                                                            cGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAgg            >  1:2243335/1‑93 (MQ=255)

                                                                                tcaggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACt        >  1:4736541/1‑93 (MQ=255)

                                                                                 caggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACtt       >  1:2078457/1‑93 (MQ=255)

                                                                                 caggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACtt       <  2:799728/93‑1 (MQ=255)

                                                                                  aggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTc      <  1:2023330/93‑1 (MQ=255)

                                                                                  aggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTc      <  1:4367308/93‑1 (MQ=255)

                                                                                   ggtcaTGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCg     >  1:6260630/1‑93 (MQ=255)

CGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCA  >  NC_000913/3588808‑3588986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAACGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACC  >  NC_000913/3588804‑3588990

TGAACGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATG                                                                                               <  SRR4302533.5623131/94‑1 (MQ=60)

 GAACGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGC                                                                                              <  SRR4302533.3731047/94‑1 (MQ=60)

 GAACGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGC                                                                                              >  SRR4302533.3878422/1‑94 (MQ=60)

 GAACGGCAACGGCCGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGC                                                                                              <  SRR4302533.5286984/94‑1 (MQ=60)

  AACGGCAACGTCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCG                                                                                             <  SRR4302533.5189578/94‑1 (MQ=60)

    CGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCT                                                                                           >  SRR4302533.4372397/1‑94 (MQ=60)

    CGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCT                                                                                           <  SRR4302533.2241563/94‑1 (MQ=60)

    CGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCT                                                                                           <  SRR4302533.20403/94‑1 (MQ=60)

     GGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTG                                                                                          <  SRR4302533.4413939/94‑1 (MQ=60)

       CAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCT                                                                                        <  SRR4302533.4626660/94‑1 (MQ=60)

       CAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCT                                                                                        >  SRR4302533.5898593/1‑94 (MQ=60)

        AACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTT                                                                                       >  SRR4302533.6225989/1‑94 (MQ=60)

         ACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTT                                                                                      >  SRR4302533.4733497/1‑94 (MQ=60)

          CGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTG                                                                                     <  SRR4302533.5922170/94‑1 (MQ=60)

          CGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTG                                                                                     <  SRR4302533.5443677/94‑1 (MQ=60)

          CGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTG                                                                                     <  SRR4302533.4052389/94‑1 (MQ=60)

          CGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTG                                                                                     >  SRR4302533.1443072/1‑94 (MQ=60)

           GGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGG                                                                                    <  SRR4302533.1894618/94‑1 (MQ=60)

            GCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGT                                                                                   <  SRR4302533.6337590/94‑1 (MQ=60)

             CGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTG                                                                                  <  SRR4302533.2326803/94‑1 (MQ=60)

             CGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTG                                                                                  <  SRR4302533.5520636/94‑1 (MQ=60)

             CGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTG                                                                                  <  SRR4302533.4841726/94‑1 (MQ=60)

              GGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGA                                                                                 >  SRR4302533.1936284/1‑94 (MQ=60)

              GGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGA                                                                                 >  SRR4302533.3969091/1‑94 (MQ=60)

                CTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATT                                                                               >  SRR4302533.2610836/1‑94 (MQ=60)

                 TTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATATAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTG                                                                              <  SRR4302533.4467177/94‑1 (MQ=60)

                 TTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCATGGGTCAGGTCATGCGCTGCTTTGGTGATTG                                                                              >  SRR4302533.1927443/1‑94 (MQ=60)

                  TATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGG                                                                             >  SRR4302533.410801/1‑94 (MQ=60)

                  TATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGG                                                                             <  SRR4302533.15067/94‑1 (MQ=60)

                   ATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGG                                                                            >  SRR4302533.5968096/1‑94 (MQ=60)

                    TTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCA                                                                           >  SRR4302533.6053563/1‑94 (MQ=60)

                     TCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAG                                                                          >  SRR4302533.428311/1‑94 (MQ=60)

                     TCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAG                                                                          <  SRR4302533.738869/94‑1 (MQ=60)

                       ATCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAT                                                                        <  SRR4302533.6089436/94‑1 (MQ=60)

                       AGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAT                                                                        >  SRR4302533.3603144/1‑94 (MQ=60)

                       AGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAT                                                                        >  SRR4302533.5939510/1‑94 (MQ=60)

                        GCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATA                                                                       <  SRR4302533.2413923/94‑1 (MQ=60)

                         CATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAA                                                                      <  SRR4302533.3725391/94‑1 (MQ=60)

                          ATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAC                                                                     <  SRR4302533.4982664/94‑1 (MQ=60)

                          ATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAC                                                                     >  SRR4302533.4426151/1‑94 (MQ=60)

                          ATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAAC                                                                     >  SRR4302533.2996189/1‑94 (MQ=60)

                           TCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACC                                                                    <  SRR4302533.3865853/94‑1 (MQ=60)

                            CTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCG                                                                   <  SRR4302533.2392068/94‑1 (MQ=60)

                            CTGACGCGTCGCGGTATCCGCCCCTGGGGTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCG                                                                   >  SRR4302533.3968068/1‑94 (MQ=60)

                             TGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGG                                                                  <  SRR4302533.3651976/94‑1 (MQ=60)

                               ACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTT                                                                <  SRR4302533.5240564/94‑1 (MQ=60)

                                CGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTT                                                               <  SRR4302533.5328912/94‑1 (MQ=60)

                                  CGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTC                                                             >  SRR4302533.4321030/1‑94 (MQ=60)

                                  CGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTC                                                             >  SRR4302533.3341197/1‑94 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTTACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            >  SRR4302533.4473709/1‑94 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            <  SRR4302533.3861019/94‑1 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            >  SRR4302533.1057149/1‑94 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            <  SRR4302533.6322394/94‑1 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            <  SRR4302533.4178039/94‑1 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            <  SRR4302533.1097124/94‑1 (MQ=60)

                                   GTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTAATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCT                                                            <  SRR4302533.3379926/94‑1 (MQ=60)

                                     CGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGA                                                          >  SRR4302533.6050565/1‑94 (MQ=60)

                                     CGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGA                                                          <  SRR4302533.3001083/94‑1 (MQ=60)

                                        GGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGC                                                       <  SRR4302533.41509/94‑1 (MQ=60)

                                           ATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCAC                                                    >  SRR4302533.224150/1‑94 (MQ=60)

                                            TCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACT                                                   <  SRR4302533.6220216/94‑1 (MQ=60)

                                              CGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCG                                                 <  SRR4302533.1722910/94‑1 (MQ=60)

                                              CGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCG                                                 >  SRR4302533.339382/1‑94 (MQ=60)

                                               GCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGG                                                <  SRR4302533.5273374/94‑1 (MQ=60)

                                                CCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGC                                               >  SRR4302533.4182205/1‑94 (MQ=60)

                                                CCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGC                                               >  SRR4302533.5724371/1‑94 (MQ=60)

                                                CCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGC                                               >  SRR4302533.2443820/1‑94 (MQ=60)

                                                 CCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCA                                              >  SRR4302533.6269034/1‑94 (MQ=60)

                                                 CCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCA                                              <  SRR4302533.1836640/94‑1 (MQ=60)

                                                 CCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCA                                              <  SRR4302533.932948/94‑1 (MQ=60)

                                                 CCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCA                                              >  SRR4302533.3490802/1‑94 (MQ=60)

                                                  CCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAG                                             >  SRR4302533.3898559/1‑94 (MQ=60)

                                                  CCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAG                                             <  SRR4302533.4346771/94‑1 (MQ=60)

                                                  CCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAG                                             >  SRR4302533.1793083/1‑94 (MQ=60)

                                                  CCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAG                                             >  SRR4302533.616879/1‑94 (MQ=60)

                                                   CTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGC                                            <  SRR4302533.5864840/94‑1 (MQ=60)

                                                    TGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTTGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCG                                           >  SRR4302533.634131/1‑94 (MQ=60)

                                                     GGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGT                                          <  SRR4302533.2646210/94‑1 (MQ=60)

                                                      GGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTT                                         >  SRR4302533.646586/1‑94 (MQ=60)

                                                      GGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTT                                         >  SRR4302533.723449/1‑94 (MQ=60)

                                                       GTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTT                                        >  SRR4302533.152254/1‑94 (MQ=60)

                                                       GTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTT                                        >  SRR4302533.2480039/1‑94 (MQ=60)

                                                       GTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGAAAACCGGTTTTCTGATGCCACTCGGCAGCGTTT                                        >  SRR4302533.6119965/1‑94 (MQ=60)

                                                        TTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTT                                       >  SRR4302533.6241612/1‑94 (MQ=60)

                                                        TTTTTCTCATAAAAGCCCTGCTCACGGGACAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTT                                       >  SRR4302533.4008065/1‑94 (MQ=60)

                                                         TTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTC                                      >  SRR4302533.2117112/1‑94 (MQ=60)

                                                         TTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTC                                      <  SRR4302533.3620714/94‑1 (MQ=60)

                                                         TTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGGTTTCTGATGCCACTCGGCAGCGTTTTT                                      >  SRR4302533.4306849/1‑94 (MQ=60)

                                                          TTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCT                                     >  SRR4302533.180287/1‑94 (MQ=60)

                                                          TTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCT                                     >  SRR4302533.5969635/1‑94 (MQ=60)

                                                           TTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTG                                    <  SRR4302533.2580587/94‑1 (MQ=60)

                                                           TTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTG                                    >  SRR4302533.273044/1‑94 (MQ=60)

                                                           TTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTG                                    >  SRR4302533.139617/1‑94 (MQ=60)

                                                            TCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGG                                   <  SRR4302533.337526/94‑1 (MQ=60)

                                                            TCTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGG                                   <  SRR4302533.4072150/94‑1 (MQ=60)

                                                             CTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGC                                  >  SRR4302533.4248779/1‑94 (MQ=60)

                                                             CTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGC                                  >  SRR4302533.3795030/1‑94 (MQ=60)

                                                             CTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGC                                  >  SRR4302533.833611/1‑94 (MQ=60)

                                                             CTCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGC                                  <  SRR4302533.1719457/94‑1 (MQ=60)

                                                              TCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCT                                 >  SRR4302533.3788286/1‑94 (MQ=60)

                                                              TCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCT                                 <  SRR4302533.3649638/94‑1 (MQ=60)

                                                              TCATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTCGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTGGGCAGCGTTTTCTCGCT                                 >  SRR4302533.1232644/1‑94 (MQ=60)

                                                               CATAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTT                                <  SRR4302533.4035348/94‑1 (MQ=60)

                                                                 TAAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCG                              >  SRR4302533.844576/1‑94 (MQ=60)

                                                                  AAAAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGC                             >  SRR4302533.1997114/1‑94 (MQ=60)

                                                                    AAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGA                           <  SRR4302533.6128624/94‑1 (MQ=60)

                                                                    AAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGA                           <  SRR4302533.753143/94‑1 (MQ=60)

                                                                    AAGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGA                           >  SRR4302533.3395355/1‑94 (MQ=60)

                                                                     AGCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAG                          <  SRR4302533.4214445/94‑1 (MQ=60)

                                                                      GCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGG                         <  SRR4302533.5697711/94‑1 (MQ=60)

                                                                      GCCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGG                         >  SRR4302533.5477704/1‑94 (MQ=60)

                                                                       CCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGA                        <  SRR4302533.2471398/94‑1 (MQ=60)

                                                                       CCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGA                        >  SRR4302533.51982/1‑94 (MQ=60)

                                                                       CCCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGA                        >  SRR4302533.2366851/1‑94 (MQ=60)

                                                                        CCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAA                       >  SRR4302533.1327181/1‑94 (MQ=60)

                                                                        CCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAA                       >  SRR4302533.1042706/1‑94 (MQ=60)

                                                                        CCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAA                       >  SRR4302533.2462655/1‑94 (MQ=60)

                                                                        CCTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAA                       <  SRR4302533.1500534/94‑1 (MQ=60)

                                                                         CTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAA                      >  SRR4302533.4552286/1‑94 (MQ=60)

                                                                         CTGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTCTTCTGGCTTCGCGAGGAAA                      >  SRR4302533.886317/1‑94 (MQ=60)

                                                                          TGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAAT                     >  SRR4302533.2041678/1‑94 (MQ=60)

                                                                          TGCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAAT                     <  SRR4302533.717121/94‑1 (MQ=60)

                                                                           GCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATC                    >  SRR4302533.4634274/1‑94 (MQ=60)

                                                                           GCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATC                    <  SRR4302533.4188411/94‑1 (MQ=60)

                                                                           GCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATC                    >  SRR4302533.4340367/1‑94 (MQ=60)

                                                                           GCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATC                    >  SRR4302533.1430472/1‑94 (MQ=60)

                                                                           GCTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATC                    >  SRR4302533.6061093/1‑94 (MQ=60)

                                                                            CTCACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCG                   <  SRR4302533.3456023/94‑1 (MQ=60)

                                                                            CTCACGGGTCAGGTCATGCGCTGCTTTGGTGATGGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCG                   <  SRR4302533.364237/94‑1 (MQ=60)

                                                                              CACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAG                 <  SRR4302533.982785/94‑1 (MQ=60)

                                                                               ACGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGG                >  SRR4302533.2257201/1‑94 (MQ=60)

                                                                               ACGGGTCAGGTCATGCGCTGCTTGGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGG                <  SRR4302533.2580920/94‑1 (MQ=60)

                                                                                CGGGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGA               >  SRR4302533.2301421/1‑94 (MQ=60)

                                                                                  GGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAAC             <  SRR4302533.3889166/94‑1 (MQ=60)

                                                                                  GGTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAAC             >  SRR4302533.4590328/1‑94 (MQ=60)

                                                                                   GTCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACT            >  SRR4302533.4765261/1‑94 (MQ=60)

                                                                                    TCAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTT           >  SRR4302533.2091249/1‑94 (MQ=60)

                                                                                     CAGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTC          <  SRR4302533.3436647/94‑1 (MQ=60)

                                                                                      AGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCG         <  SRR4302533.4393673/94‑1 (MQ=60)

                                                                                      AGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCG         <  SRR4302533.2035825/94‑1 (MQ=60)

                                                                                      AGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCG         >  SRR4302533.6298060/1‑94 (MQ=60)

                                                                                      AGGTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCG         >  SRR4302533.502780/1‑94 (MQ=60)

                                                                                        GTCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCC       <  SRR4302533.3929819/94‑1 (MQ=60)

                                                                                         TCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCA      <  SRR4302533.2217011/94‑1 (MQ=60)

                                                                                         TCATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCA      <  SRR4302533.4966229/94‑1 (MQ=60)

                                                                                          CATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCAC     <  SRR4302533.3893436/94‑1 (MQ=60)

                                                                                          CATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCAC     >  SRR4302533.851573/1‑94 (MQ=60)

                                                                                           ATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACA    <  SRR4302533.1722712/94‑1 (MQ=60)

                                                                                           ATGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACA    <  SRR4302533.2537311/94‑1 (MQ=60)

                                                                                            TGCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACAC   <  SRR4302533.6190035/94‑1 (MQ=60)

                                                                                             GCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACC  <  SRR4302533.4491593/94‑1 (MQ=60)

                                                                                             GCGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACC  <  SRR4302533.2933997/94‑1 (MQ=60)

TGAACGGCAACGGCGGCTTATTCAGCATCTGACGCGTCGCGGTATCCGCCCCTGGGTTTTTCTCATAAAAGCCCTGCTCACGGGTCAGGTCATACGCTGCTTTGGTGATTGGCAGATAACCGGTTTTCTGATGCCACTCGGCAGCGTTTTCTGGCTTCGCGAGGAAATCGAGGAACTTCGCCACACC  >  NC_000913/3588804‑3588990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`