Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,665,586 T→C A411A (GCA→GCG)  yhjA ← putative cytochrome C peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,5860TC99.5% 598.2 / ‑4.8 188A411A (GCA→GCG) yhjAputative cytochrome C peroxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (89/98);  minor base A (0/1);  total (89/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 199 total aligned reads displayed.

CTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGC  >  NC_000913/3665496‑3665675
                                                                                          |                                                                                         
ctcctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGgcgc                                                                                         >  2:5307510/1‑93 (MQ=255)
 tcctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGcc                                                                                        >  1:1048966/1‑93 (MQ=255)
  cctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCg                                                                                       >  1:5695323/1‑93 (MQ=255)
  cctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCg                                                                                       <  1:4169301/93‑1 (MQ=255)
   ctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGt                                                                                      <  1:5463963/93‑1 (MQ=255)
   ctGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGt                                                                                      >  1:2649531/1‑93 (MQ=255)
      cGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGttca                                                                                   <  2:5011248/93‑3 (MQ=255)
       ggCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTaaa                                                                                  <  1:1758037/93‑1 (MQ=255)
        gCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAg                                                                                 >  2:4270849/1‑93 (MQ=255)
          aGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCa                                                                               >  2:5371025/1‑93 (MQ=255)
          aGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCa                                                                               <  2:5283231/93‑1 (MQ=255)
          aGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCa                                                                               <  1:5937253/93‑1 (MQ=255)
           gCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCaa                                                                              >  2:3109720/1‑93 (MQ=255)
            ctctTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAAc                                                                             <  2:2765162/93‑1 (MQ=255)
              ctTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGt                                                                           >  1:4411119/1‑93 (MQ=255)
              ctTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGt                                                                           <  1:153119/93‑1 (MQ=255)
               tttGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGtt                                                                          <  1:4717336/93‑1 (MQ=255)
                    cTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTa                                                                     <  2:3561131/93‑1 (MQ=255)
                    cTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTa                                                                     >  2:2382686/1‑93 (MQ=255)
                     tACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTaa                                                                    <  2:1052400/93‑1 (MQ=255)
                     tACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTaa                                                                    >  2:6224826/1‑93 (MQ=255)
                     tACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTGACGTaa                                                                    >  1:4948889/1‑93 (MQ=255)
                     tACCTGATAGCGCAGCATCAGTTTCACAGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGtga                                                                    >  1:4899564/1‑91 (MQ=255)
                      aCCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTaaa                                                                   >  1:3475413/1‑93 (MQ=255)
                      aCCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTaaa                                                                   >  2:2720575/1‑93 (MQ=255)
                       ccTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAAc                                                                  <  2:941050/93‑1 (MQ=255)
                       ccTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAAc                                                                  <  2:5799894/93‑1 (MQ=255)
                         tGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCg                                                                >  1:4390141/1‑93 (MQ=255)
                         tGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCg                                                                >  2:825098/1‑93 (MQ=255)
                           aTAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCggg                                                              <  2:1239150/93‑1 (MQ=255)
                             aGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTa                                                            <  2:2783544/93‑1 (MQ=255)
                                gcagcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTAcgt                                                         >  2:3255341/1‑91 (MQ=255)
                                 cagcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAAc                                                                  <  1:94606/83‑1 (MQ=255)
                                  agcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTc                                                       <  2:2071307/93‑1 (MQ=255)
                                   gcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCt                                                      <  2:422046/93‑1 (MQ=255)
                                   gcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCt                                                      <  2:1623106/93‑1 (MQ=255)
                                     aTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGa                                                    <  2:3882890/93‑1 (MQ=255)
                                      tCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGAc                                                   >  2:260440/1‑93 (MQ=255)
                                        aGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGc                                                 <  2:1919244/93‑1 (MQ=255)
                                         gTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCa                                                <  1:5615859/93‑1 (MQ=255)
                                          tttCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCaa                                               <  1:4191764/93‑1 (MQ=255)
                                          tttCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCaa                                               >  2:3675375/1‑93 (MQ=255)
                                                cGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATc                                         >  1:2218060/1‑93 (MQ=255)
                                                 gCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCa                                        <  1:712802/93‑1 (MQ=255)
                                                  ccccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCAc                                       >  2:2979654/1‑93 (MQ=255)
                                                  ccccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCAc                                       <  2:1704234/93‑1 (MQ=255)
                                                   cccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACg                                      >  1:4541293/1‑93 (MQ=255)
                                                    ccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGc                                     <  2:1394393/93‑1 (MQ=255)
                                                    ccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGc                                     >  1:4026260/1‑93 (MQ=255)
                                                    ccGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGc                                     >  1:2183362/1‑93 (MQ=255)
                                                      gTCCGGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGctc                                   <  1:5573745/93‑1 (MQ=255)
                                                      gTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAACCCGGGTACTTTCTGACGCAATTTATCACGctc                                   <  2:5139460/93‑1 (MQ=255)
                                                       tCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGctct                                  >  2:154774/1‑93 (MQ=255)
                                                         cAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCtcttc                                >  1:269951/1‑93 (MQ=255)
                                                          aGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCtcttct                               >  2:5848147/1‑93 (MQ=255)
                                                           gCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTcttctt                              <  1:6111686/93‑1 (MQ=255)
                                                           gCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTcttctt                              >  1:3079607/1‑93 (MQ=255)
                                                           gCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTcttctt                              >  2:6172409/1‑93 (MQ=255)
                                                            cGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTGTCACGCTCTTCttt                             <  2:3850354/93‑1 (MQ=255)
                                                            cGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCttt                             <  2:3396161/93‑1 (MQ=255)
                                                             gTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTa                            <  1:2567303/93‑1 (MQ=255)
                                                             gTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTa                            <  2:1850572/93‑1 (MQ=255)
                                                                ggCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTc                         >  2:5608404/1‑93 (MQ=255)
                                                                    cGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACAt                     <  2:5915852/93‑1 (MQ=255)
                                                                     gTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACAtt                    >  1:487869/1‑93 (MQ=255)
                                                                     gTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACAtt                    >  2:3721340/1‑93 (MQ=255)
                                                                      tCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTc                   <  2:3961075/93‑1 (MQ=255)
                                                                        aCCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAt                 <  1:3839735/93‑1 (MQ=255)
                                                                        aCCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAt                 <  2:745312/93‑1 (MQ=255)
                                                                        aCCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAt                 <  2:261904/93‑1 (MQ=255)
                                                                        aCCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAt                 <  1:5871307/93‑1 (MQ=255)
                                                                         ccGCGATGGAAGTACGGCGCCGTTAAGGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATa                <  1:4989976/93‑1 (MQ=255)
                                                                          cgcgATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAc               >  1:4571420/1‑93 (MQ=255)
                                                                          cgcgATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAc               <  2:4160326/93‑1 (MQ=255)
                                                                            cgATGGAAGGACGGCGCAGTAAAAGCAACGTTAAGTAAGCCGGTTTCTTTCTGACTTAATTTATCACGCTTTTCTTTAGTCACATTCATACGa             <  1:5225671/93‑1 (MQ=255)
                                                                             gATGGACGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAc            <  1:1249570/93‑1 (MQ=255)
                                                                             gATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAc            >  1:2876819/1‑93 (MQ=255)
                                                                             gATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAc            >  2:601455/1‑93 (MQ=255)
                                                                              aTGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAct           <  2:6092067/93‑2 (MQ=255)
                                                                              aTGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAcc           >  1:447424/1‑93 (MQ=255)
                                                                              aTGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAcc           >  1:1352978/1‑93 (MQ=255)
                                                                              aTGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAcc           >  1:4651301/1‑93 (MQ=255)
                                                                              aTGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAcc           >  2:313507/1‑93 (MQ=255)
                                                                                ggAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCaa         <  2:4631462/93‑1 (MQ=255)
                                                                                ggAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCaa         <  2:5621924/93‑1 (MQ=255)
                                                                                 gAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAat        >  1:3561632/1‑93 (MQ=255)
                                                                                 gAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAat        <  2:4392639/93‑1 (MQ=255)
                                                                                 gAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAat        <  2:3440209/93‑1 (MQ=255)
                                                                                  aaGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAata       >  1:2753421/1‑93 (MQ=255)
                                                                                   aGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAatat      >  1:4283845/1‑93 (MQ=255)
                                                                                   aGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAatat      >  2:808881/1‑93 (MQ=255)
                                                                                    gTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATc     <  1:1455451/93‑1 (MQ=255)
                                                                                    gTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATc     >  2:2466652/1‑93 (MQ=255)
                                                                                    gTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATc     <  2:3309512/93‑1 (MQ=255)
                                                                                    gTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATc     <  1:1990339/93‑1 (MQ=255)
                                                                                    gTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATc     <  2:4880902/93‑1 (MQ=255)
                                                                                     tACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATcc    <  1:6180097/93‑1 (MQ=255)
                                                                                     tACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATcc    <  2:2678511/93‑1 (MQ=255)
                                                                                      aCGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATccg   >  1:1288396/1‑93 (MQ=255)
                                                                                       cGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATccgc  <  1:3127059/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGC  >  NC_000913/3665496‑3665675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCC  >  NC_000913/3665493‑3665679
                                                                                             |                                                                                             
ATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGC                                                                                               >  SRR4302533.6291466/1‑94 (MQ=60)
ATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGC                                                                                               >  SRR4302533.6128880/1‑94 (MQ=60)
 TCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCG                                                                                              >  SRR4302533.789403/1‑94 (MQ=60)
 TCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCG                                                                                              <  SRR4302533.1837671/94‑1 (MQ=60)
  CCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGC                                                                                             >  SRR4302533.4539413/1‑94 (MQ=60)
   CTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCC                                                                                            >  SRR4302533.1055134/1‑94 (MQ=60)
   CTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCC                                                                                            >  SRR4302533.4579435/1‑94 (MQ=60)
   CTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCC                                                                                            <  SRR4302533.5634906/94‑1 (MQ=60)
    TCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCG                                                                                           >  SRR4302533.5729719/1‑94 (MQ=60)
     CCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGT                                                                                          <  SRR4302533.4194567/94‑1 (MQ=60)
     CCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGT                                                                                          >  SRR4302533.2665806/1‑94 (MQ=60)
      CTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTT                                                                                         <  SRR4302533.5496889/94‑1 (MQ=60)
       TGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTA                                                                                        >  SRR4302533.4694220/1‑94 (MQ=60)
          GGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAG                                                                                     <  SRR4302533.1768754/94‑1 (MQ=60)
          GGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAG                                                                                     <  SRR4302533.727321/94‑1 (MQ=60)
            CAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGCACGGCGCCGTTAAAGCA                                                                                   >  SRR4302533.528146/1‑94 (MQ=60)
             AGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAA                                                                                  <  SRR4302533.5973151/94‑1 (MQ=60)
              GCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAAC                                                                                 <  SRR4302533.6177522/94‑1 (MQ=60)
               CTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACG                                                                                >  SRR4302533.134338/1‑94 (MQ=60)
                TCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGT                                                                               >  SRR4302533.4437761/1‑94 (MQ=60)
                TCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGT                                                                               >  SRR4302533.2204536/1‑94 (MQ=60)
                 CTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTT                                                                              >  SRR4302533.962281/1‑94 (MQ=60)
                 CTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTT                                                                              <  SRR4302533.154098/94‑1 (MQ=60)
                  TTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTA                                                                             <  SRR4302533.4745941/94‑1 (MQ=60)
                   TTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTAC                                                                            <  SRR4302533.350334/94‑1 (MQ=60)
                    TGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACG                                                                           <  SRR4302533.4498686/94‑1 (MQ=60)
                       CTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAA                                                                        >  SRR4302533.3131409/1‑94 (MQ=60)
                       CTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTGACGTAA                                                                        >  SRR4302533.4978859/1‑94 (MQ=60)
                       CTACCTGATAGCGCAGCATCAGTTTCACAGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTGA                                                                        >  SRR4302533.4929248/1‑94 (MQ=60)
                        TACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAA                                                                       >  SRR4302533.2622968/1‑94 (MQ=60)
                        TACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAA                                                                       >  SRR4302533.3496859/1‑94 (MQ=60)
                          ctgctgtatGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACC                                                                     <  SRR4302533.95202/85‑1 (MQ=60)
                           CTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCG                                                                    >  SRR4302533.4416647/1‑94 (MQ=60)
                              ATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGT                                                                 >  SRR4302533.4851606/1‑94 (MQ=60)
                                      GCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTG                                                         >  SRR4302533.2746780/1‑94 (MQ=60)
                                        ATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGAC                                                       >  SRR4302533.4027474/1‑94 (MQ=60)
                                        ATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGAC                                                       >  SRR4302533.614525/1‑94 (MQ=60)
                                           AGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCA                                                    >  SRR4302533.5014542/1‑94 (MQ=60)
                                            GTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAA                                                   <  SRR4302533.5649806/94‑1 (MQ=60)
                                             TTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAAT                                                  <  SRR4302533.4217134/94‑1 (MQ=60)
                                             TTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAAT                                                  >  SRR4302533.5893392/1‑94 (MQ=60)
                                               TCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTT                                                <  SRR4302533.3707985/94‑1 (MQ=60)
                                                CACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTA                                               >  SRR4302533.4924728/1‑94 (MQ=60)
                                                  CCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATC                                             >  SRR4302533.2231753/1‑94 (MQ=60)
                                                    GCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCAC                                           <  SRR4302533.717009/94‑1 (MQ=60)
                                                    GCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCAC                                           >  SRR4302533.2029905/1‑94 (MQ=60)
                                                     CCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACG                                          >  SRR4302533.4568791/1‑94 (MQ=60)
                                                      CCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGC                                         >  SRR4302533.4050770/1‑94 (MQ=60)
                                                      CCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGC                                         >  SRR4302533.2196825/1‑94 (MQ=60)
                                                       CCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCT                                        <  SRR4302533.4400367/94‑1 (MQ=60)
                                                         GTCCGGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCT                                      <  SRR4302533.5607430/94‑1 (MQ=60)
                                                           CCAGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTC                                    >  SRR4302533.271591/1‑94 (MQ=60)
                                                             AGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTT                                  >  SRR4302533.4649345/1‑94 (MQ=60)
                                                             AGCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTT                                  >  SRR4302533.3098504/1‑94 (MQ=60)
                                                              GCGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTT                                 <  SRR4302533.6148576/94‑1 (MQ=60)
                                                               CGTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTA                                <  SRR4302533.5084795/94‑1 (MQ=60)
                                                                GTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAG                               >  SRR4302533.5936574/1‑94 (MQ=60)
                                                                GTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAG                               <  SRR4302533.199875/94‑1 (MQ=60)
                                                                GTCGGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAG                               <  SRR4302533.2583101/94‑1 (MQ=60)
                                                                   GGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCA                            <  SRR4302533.2860002/94‑1 (MQ=60)
                                                                   GGCACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCA                            <  SRR4302533.64447/94‑1 (MQ=60)
                                                                     CACGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACA                          >  SRR4302533.150130/1‑94 (MQ=60)
                                                                       CGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATT                        >  SRR4302533.490786/1‑94 (MQ=60)
                                                                       CGTCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATT                        <  SRR4302533.1715512/94‑1 (MQ=60)
                                                                         TCACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCA                      <  SRR4302533.4950355/94‑1 (MQ=60)
                                                                          CACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAT                     <  SRR4302533.1379056/94‑1 (MQ=60)
                                                                          CACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCAT                     <  SRR4302533.2548159/94‑1 (MQ=60)
                                                                           ACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATA                    <  SRR4302533.5906793/94‑1 (MQ=60)
                                                                           ACCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATA                    <  SRR4302533.3863193/94‑1 (MQ=60)
                                                                            CCGCGATGGAAGTACGGCGCCGTTAAGGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAC                   <  SRR4302533.5020229/94‑1 (MQ=60)
                                                                            CCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAC                   <  SRR4302533.5698416/94‑1 (MQ=60)
                                                                            CCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAC                   >  SRR4302533.4599124/1‑94 (MQ=60)
                                                                            CCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAC                   <  SRR4302533.4198803/94‑1 (MQ=60)
                                                                            CCGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATAC                   <  SRR4302533.4423246/94‑1 (MQ=60)
                                                                             CGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACG                  <  SRR4302533.3433610/94‑1 (MQ=60)
                                                                             CGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACG                  <  SRR4302533.1295368/94‑1 (MQ=60)
                                                                             CGCGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACG                  <  SRR4302533.4092809/94‑1 (MQ=60)
                                                                               CGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAC                >  SRR4302533.2894522/1‑94 (MQ=60)
                                                                               CGATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGAC                >  SRR4302533.42210/1‑94 (MQ=60)
                                                                               CGATGGAAGGACGGCGCAGTAAAAGCAACGTTAAGTAAGCCGGTTTCTTTCTGACTTAATTTATCACGCTTTTCTTTAGTCACATTCATACGAC                <  SRR4302533.5257117/94‑1 (MQ=32)
                                                                                GATGGACGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACC               <  SRR4302533.1257033/94‑1 (MQ=60)
                                                                                GATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACC               >  SRR4302533.450100/1‑94 (MQ=60)
                                                                                GATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACC               >  SRR4302533.1361114/1‑94 (MQ=60)
                                                                                GATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACC               >  SRR4302533.4679483/1‑94 (MQ=60)
                                                                                GATGGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACC               >  SRR4302533.2711007/1‑94 (MQ=60)
                                                                                   GGAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAAT            >  SRR4302533.3583578/1‑94 (MQ=60)
                                                                                    GAAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATA           >  SRR4302533.2770305/1‑94 (MQ=60)
                                                                                     AAGTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATAT          >  SRR4302533.4309589/1‑94 (MQ=60)
                                                                                       GTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCC        <  SRR4302533.2919870/94‑1 (MQ=60)
                                                                                       GTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCC        <  SRR4302533.1464225/94‑1 (MQ=60)
                                                                                       GTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCC        <  SRR4302533.2002528/94‑1 (MQ=60)
                                                                                       GTACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCC        <  SRR4302533.5553513/94‑1 (MQ=60)
                                                                                        TACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCG       <  SRR4302533.6217212/94‑1 (MQ=60)
                                                                                        TACGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCG       >  SRR4302533.1296149/1‑94 (MQ=60)
                                                                                          CGGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCC     <  SRR4302533.3146283/94‑1 (MQ=60)
                                                                                           GGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCG    >  SRR4302533.5661356/1‑94 (MQ=60)
                                                                                           GGCGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCG    <  SRR4302533.2673802/94‑1 (MQ=60)
                                                                                             CGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCC  >  SRR4302533.6124640/1‑94 (MQ=60)
                                                                                             CGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCC  <  SRR4302533.2046336/94‑1 (MQ=60)
                                                                                             CGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCC  <  SRR4302533.2434296/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCC  >  NC_000913/3665493‑3665679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑