Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,667,150 A→G intergenic (‑332/‑72) yhjA ← / → treF putative cytochrome C peroxidase/cytoplasmic trehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,667,1500AG100.0% 791.0 / NA 245intergenic (‑332/‑72)yhjA/treFputative cytochrome C peroxidase/cytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (143/102);  total (143/102)

Only 100 of 256 total aligned reads displayed.

AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATT  >  NC_000913/3667060‑3667239
                                                                                          |                                                                                         
 aaTCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCccgcca                                                                                        >  1:6117010/1‑93 (MQ=255)
 aaTCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCccgcca                                                                                        >  1:1960974/1‑93 (MQ=255)
   tCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAgg                                                                                      <  1:1396427/93‑1 (MQ=255)
    cGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGc                                                                                     >  2:4303937/1‑93 (MQ=255)
    cGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGc                                                                                     <  2:521709/93‑1 (MQ=255)
      gTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCgg                                                                                   >  2:1879130/1‑93 (MQ=255)
       tATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGa                                                                                  >  1:4283992/1‑93 (MQ=255)
         tttttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGc                                                                                >  1:1005097/1‑93 (MQ=255)
         tttttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGc                                                                                >  2:5540297/1‑93 (MQ=255)
         tttttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGc                                                                                <  1:1516782/93‑1 (MQ=255)
           tttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGa                                                                              >  1:3891553/1‑93 (MQ=255)
            ttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGaa                                                                             >  2:415470/1‑93 (MQ=255)
            ttATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGaa                                                                             <  1:5108569/93‑1 (MQ=255)
             tataAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAAt                                                                            >  2:51730/1‑93 (MQ=255)
              ataAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATg                                                                           >  2:5243535/1‑93 (MQ=255)
               taAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGa                                                                          >  2:5398817/1‑93 (MQ=255)
                aaaTTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATGCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGAc                                                                         >  1:4138096/1‑93 (MQ=255)
                  aTTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTa                                                                       >  1:2973643/1‑93 (MQ=255)
                  aTTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTa                                                                       >  1:2932950/1‑93 (MQ=255)
                     ccATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTAccc                                                                    >  2:2315503/1‑93 (MQ=255)
                      cATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCt                                                                   >  2:5372580/1‑93 (MQ=255)
                        ttGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCTCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTa                                                                 <  1:3377815/93‑1 (MQ=255)
                        ttGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTa                                                                 >  1:2324769/1‑93 (MQ=255)
                         tGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTaa                                                                >  2:1022174/1‑93 (MQ=255)
                          gATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTaaa                                                               <  2:915779/93‑1 (MQ=255)
                          gATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTaaa                                                               >  1:1101550/1‑93 (MQ=255)
                           atatTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAg                                                              >  2:4943742/1‑93 (MQ=255)
                           atatTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAg                                                              >  1:5433535/1‑93 (MQ=255)
                           atatTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAg                                                              >  1:2129893/1‑93 (MQ=255)
                           atatTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAg                                                              <  2:80933/93‑1 (MQ=255)
                             atTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaa                                                            >  1:4169673/1‑93 (MQ=255)
                              ttAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaaa                                                           <  2:3814181/93‑1 (MQ=255)
                              ttAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaaa                                                           >  1:3570928/1‑93 (MQ=255)
                              ttAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaaa                                                           >  2:2033100/1‑93 (MQ=255)
                              ttAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaaa                                                           <  2:2629109/93‑1 (MQ=255)
                               tAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGaaaa                                                          <  1:5374410/93‑1 (MQ=255)
                                aGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAg                                                         <  1:2544019/93‑1 (MQ=255)
                                 gTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGc                                                        <  1:4445947/93‑1 (MQ=255)
                                 gTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGc                                                        <  2:2074763/93‑1 (MQ=255)
                                  tGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGcc                                                       >  1:905595/1‑93 (MQ=255)
                                  tGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGcc                                                       >  2:5980807/1‑93 (MQ=255)
                                  tGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGcc                                                       >  1:37538/1‑93 (MQ=255)
                                     gTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGAt                                                    >  2:5288877/1‑93 (MQ=255)
                                     gTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGAt                                                    >  2:1171630/1‑93 (MQ=255)
                                      ttATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATa                                                   >  2:3416483/1‑93 (MQ=255)
                                       tataTTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATaa                                                  >  2:797955/1‑93 (MQ=255)
                                       tataTTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATaa                                                  <  2:3597447/93‑1 (MQ=255)
                                          atTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTa                                               <  1:5257941/93‑1 (MQ=255)
                                          atTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTa                                               >  2:24340/1‑93 (MQ=255)
                                             ttttGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGcg                                            >  1:5631876/1‑93 (MQ=255)
                                                tGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGcgacg                                         >  1:3876202/1‑93 (MQ=255)
                                                  aCAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGaa                                       <  2:2400684/93‑1 (MQ=255)
                                                   cAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAAt                                      <  2:6202446/93‑1 (MQ=255)
                                                    aGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAAtt                                     >  1:2893887/1‑93 (MQ=255)
                                                    aGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAAtt                                     <  2:1312039/93‑1 (MQ=255)
                                                      gTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTc                                   <  1:5007582/93‑1 (MQ=255)
                                                      gTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTc                                   <  2:4030356/93‑1 (MQ=255)
                                                      gTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTc                                   >  2:1537455/1‑93 (MQ=255)
                                                       ttAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCg                                  <  2:6053415/93‑1 (MQ=255)
                                                       ttAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCg                                  >  2:4533635/1‑93 (MQ=255)
                                                         aataaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGa                                <  1:242590/93‑1 (MQ=255)
                                                         aataaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGa                                >  1:1783822/1‑93 (MQ=255)
                                                          ataaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAg                               >  1:3719756/1‑93 (MQ=255)
                                                          ataaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAg                               >  2:1283061/1‑93 (MQ=255)
                                                           taaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAgg                              <  2:5667008/93‑1 (MQ=255)
                                                           taaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAgg                              >  2:2507968/1‑93 (MQ=255)
                                                           taaACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAgg                              >  1:1853930/1‑93 (MQ=255)
                                                            aaaCGTGATCCCCCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGt                             <  1:5213524/93‑1 (MQ=255)
                                                            aaaCGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGt                             >  2:1742147/1‑93 (MQ=255)
                                                            aaaCGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGt                             >  2:306175/1‑93 (MQ=255)
                                                             aaCGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGtt                            >  1:531580/1‑93 (MQ=255)
                                                              aCGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTg                           <  1:1730943/93‑1 (MQ=255)
                                                              aCGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTg                           >  1:4956809/1‑93 (MQ=255)
                                                               cGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTgg                          <  1:3550204/93‑1 (MQ=255)
                                                                gTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGa                         >  1:4421000/1‑93 (MQ=255)
                                                                gTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGa                         >  1:2375260/1‑93 (MQ=255)
                                                                gTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGa                         <  1:4820457/93‑1 (MQ=255)
                                                                  gATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATc                       >  2:2653954/1‑93 (MQ=255)
                                                                  gATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATc                       >  1:4522029/1‑93 (MQ=255)
                                                                   aTCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATcc                      >  2:2823826/1‑93 (MQ=255)
                                                                   aTCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATcc                      <  2:698193/93‑1 (MQ=255)
                                                                      cACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTa                   >  1:6308259/1‑93 (MQ=255)
                                                                      cACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTa                   <  2:4135273/93‑1 (MQ=255)
                                                                       aCCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAt                  >  1:1379396/1‑93 (MQ=255)
                                                                       aCCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAt                  >  2:3878087/1‑93 (MQ=255)
                                                                        ccGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATg                 <  2:228840/93‑1 (MQ=255)
                                                                         cGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGc                >  1:2626679/1‑93 (MQ=255)
                                                                          gCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCt               <  1:648590/93‑1 (MQ=255)
                                                                           cACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTc              >  2:2716766/1‑93 (MQ=255)
                                                                           cACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTc              <  1:4897577/93‑1 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         <  1:2177461/93‑1 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         >  2:4072310/1‑93 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         <  1:274412/93‑1 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         <  1:5711319/93‑1 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         >  1:3146196/1‑93 (MQ=255)
                                                                                tttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCa         <  1:3287410/93‑1 (MQ=255)
                                                                                 ttGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAg        <  1:2805379/93‑1 (MQ=255)
                                                                                   gTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGaa      >  1:903692/1‑93 (MQ=255)
                                                                                    tCGCCCGCAAGGCGAAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGATTTTCGGAGGTTGGATCCTAATGCTCAATCAGaaa     <  2:5583684/93‑1 (MQ=255)
                                                                                      gCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAAt   <  1:1660635/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATT  >  NC_000913/3667060‑3667239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAA  >  NC_000913/3667057‑3667243
                                                                                             |                                                                                             
ACCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCG                                                                                               <  SRR4302533.3653338/94‑1 (MQ=60)
 CCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGC                                                                                              >  SRR4302533.2959814/1‑94 (MQ=60)
 CCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGC                                                                                              >  SRR4302533.365503/1‑94 (MQ=60)
 CCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGC                                                                                              >  SRR4302533.3032033/1‑94 (MQ=60)
   AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCA                                                                                            >  SRR4302533.1972944/1‑94 (MQ=60)
   AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCA                                                                                            >  SRR4302533.6153910/1‑94 (MQ=60)
   AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCA                                                                                            <  SRR4302533.514634/94‑1 (MQ=60)
   AAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCA                                                                                            <  SRR4302533.5297396/94‑1 (MQ=60)
    AATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAG                                                                                           <  SRR4302533.610686/94‑1 (MQ=60)
     ATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGG                                                                                          >  SRR4302533.1402483/1‑94 (MQ=60)
      TCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGC                                                                                         <  SRR4302533.1404845/94‑1 (MQ=60)
        GGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGG                                                                                       >  SRR4302533.2748021/1‑94 (MQ=60)
         GTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGA                                                                                      >  SRR4302533.4309736/1‑94 (MQ=60)
         GTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGA                                                                                      >  SRR4302533.1949054/1‑94 (MQ=60)
         GTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGA                                                                                      >  SRR4302533.4911130/1‑94 (MQ=60)
         GTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGA                                                                                      <  SRR4302533.2784119/94‑1 (MQ=60)
           ATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGC                                                                                    >  SRR4302533.1010975/1‑94 (MQ=60)
            TTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCG                                                                                   <  SRR4302533.1525915/94‑1 (MQ=60)
             TTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGA                                                                                  >  SRR4302533.3915300/1‑94 (MQ=60)
              TTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAA                                                                                 >  SRR4302533.5467052/1‑94 (MQ=60)
               TTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAAT                                                                                <  SRR4302533.1700036/94‑1 (MQ=60)
               TTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAAT                                                                                <  SRR4302533.5139436/94‑1 (MQ=60)
                TATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATG                                                                               >  SRR4302533.1960734/1‑94 (MQ=60)
                 ATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGA                                                                              >  SRR4302533.2506751/1‑94 (MQ=60)
                  TAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATGCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGAC                                                                             >  SRR4302533.4163200/1‑94 (MQ=60)
                    AATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTA                                                                           >  SRR4302533.2991837/1‑94 (MQ=60)
                    AATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTA                                                                           >  SRR4302533.2950885/1‑94 (MQ=60)
                     ATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTAC                                                                          >  SRR4302533.5392999/1‑94 (MQ=60)
                      TTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACC                                                                         <  SRR4302533.1477547/94‑1 (MQ=60)
                      TTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACC                                                                         <  SRR4302533.3550303/94‑1 (MQ=60)
                      TTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACC                                                                         >  SRR4302533.1861132/1‑94 (MQ=60)
                      TTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACC                                                                         <  SRR4302533.1138649/94‑1 (MQ=60)
                       TCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCC                                                                        >  SRR4302533.2163871/1‑94 (MQ=60)
                         CATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTT                                                                      >  SRR4302533.4535938/1‑94 (MQ=60)
                          ATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTA                                                                     >  SRR4302533.2339160/1‑94 (MQ=60)
                          ATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTA                                                                     >  SRR4302533.3445939/1‑94 (MQ=60)
                           TTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCTCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAA                                                                    <  SRR4302533.3398669/94‑1 (MQ=60)
                            TGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAA                                                                   >  SRR4302533.1108077/1‑94 (MQ=60)
                             GATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAG                                                                  >  SRR4302533.4159323/1‑94 (MQ=60)
                             GATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAG                                                                  >  SRR4302533.5466257/1‑94 (MQ=60)
                             GATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAG                                                                  >  SRR4302533.2143027/1‑94 (MQ=60)
                             GATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAG                                                                  >  SRR4302533.4333563/1‑94 (MQ=60)
                              ATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGA                                                                 >  SRR4302533.2119204/1‑94 (MQ=60)
                              ATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATACACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGA                                                                 >  SRR4302533.3395302/1‑94 (MQ=60)
                               TATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAA                                                                >  SRR4302533.2592877/1‑94 (MQ=60)
                               TATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAA                                                                >  SRR4302533.4764820/1‑94 (MQ=60)
                               TATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAA                                                                >  SRR4302533.4194940/1‑94 (MQ=60)
                                ATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAA                                                               >  SRR4302533.3592934/1‑94 (MQ=60)
                                  TAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAG                                                             <  SRR4302533.5406708/94‑1 (MQ=60)
                                   AGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGC                                                            <  SRR4302533.2559675/94‑1 (MQ=60)
                                   AGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGC                                                            <  SRR4302533.4165908/94‑1 (MQ=60)
                                    GTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCC                                                           <  SRR4302533.4472839/94‑1 (MQ=60)
                                    GTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCC                                                           >  SRR4302533.37786/1‑94 (MQ=60)
                                    GTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCC                                                           >  SRR4302533.910902/1‑94 (MQ=60)
                                      GCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGA                                                         >  SRR4302533.2158603/1‑94 (MQ=60)
                                        GTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATA                                                       <  SRR4302533.636816/94‑1 (MQ=60)
                                        GTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATA                                                       <  SRR4302533.3332064/94‑1 (MQ=60)
                                          TATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAAT                                                     >  SRR4302533.280424/1‑94 (MQ=60)
                                           ATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATT                                                    <  SRR4302533.2404951/94‑1 (MQ=60)
                                           ATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATT                                                    <  SRR4302533.5240353/94‑1 (MQ=60)
                                            TATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTA                                                   <  SRR4302533.3573634/94‑1 (MQ=60)
                                            TATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTA                                                   <  SRR4302533.4013596/94‑1 (MQ=60)
                                            TATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTA                                                   <  SRR4302533.2357764/94‑1 (MQ=60)
                                            TATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTA                                                   >  SRR4302533.5274977/1‑94 (MQ=60)
                                             ATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAG                                                  <  SRR4302533.5289513/94‑1 (MQ=60)
                                             ATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAG                                                  <  SRR4302533.2930601/94‑1 (MQ=60)
                                              TTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGC                                                 <  SRR4302533.1412969/94‑1 (MQ=60)
                                              TTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGC                                                 <  SRR4302533.2370417/94‑1 (MQ=60)
                                               TTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCG                                                <  SRR4302533.2218535/94‑1 (MQ=60)
                                               TTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCG                                                >  SRR4302533.5665934/1‑94 (MQ=60)
                                                  TTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACG                                             >  SRR4302533.3899863/1‑94 (MQ=60)
                                                   TGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGA                                            <  SRR4302533.2938595/94‑1 (MQ=60)
                                                   TGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGA                                            >  SRR4302533.3035221/1‑94 (MQ=60)
                                                   TGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGA                                            >  SRR4302533.4998386/1‑94 (MQ=60)
                                                   TGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGACGATAATTAGCGACGA                                            >  SRR4302533.5556583/1‑94 (MQ=60)
                                                     ACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAAT                                          >  SRR4302533.6302934/1‑94 (MQ=60)
                                                     ACAGGTTAATAAACGTGATCCACCGCACGCTTGGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAAT                                          <  SRR4302533.3704635/94‑1 (MQ=60)
                                                      CAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATT                                         >  SRR4302533.2911669/1‑94 (MQ=60)
                                                       AGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTT                                        <  SRR4302533.654663/94‑1 (MQ=60)
                                                        GGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTC                                       <  SRR4302533.3026541/94‑1 (MQ=60)
                                                        GGTTAATAAACGTAATCCACCGCACTCCTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTC                                       <  SRR4302533.2877510/94‑1 (MQ=60)
                                                         GTTAATAAGCGTGATCCACCGCACGCTTTGTCTCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCG                                      <  SRR4302533.1135822/94‑1 (MQ=60)
                                                         GTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCG                                      <  SRR4302533.5037925/94‑1 (MQ=60)
                                                          TTAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGG                                     <  SRR4302533.5921626/94‑1 (MQ=60)
                                                           TAATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGA                                    >  SRR4302533.1794669/1‑94 (MQ=60)
                                                            AATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAG                                   <  SRR4302533.244094/94‑1 (MQ=60)
                                                            AATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAG                                   >  SRR4302533.3742588/1‑94 (MQ=60)
                                                            AATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAG                                   <  SRR4302533.5380497/94‑1 (MQ=60)
                                                             ATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGG                                  >  SRR4302533.1865232/1‑94 (MQ=60)
                                                             ATAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGG                                  <  SRR4302533.3771315/94‑1 (MQ=60)
                                                              TAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGT                                 >  SRR4302533.662955/1‑94 (MQ=60)
                                                              TAAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGT                                 >  SRR4302533.1545991/1‑94 (MQ=60)
                                                               AAACGTGATCCCCCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                <  SRR4302533.5244920/94‑1 (MQ=60)
                                                               AAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                <  SRR4302533.254505/94‑1 (MQ=60)
                                                               AAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                >  SRR4302533.4443363/1‑94 (MQ=60)
                                                               AAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                >  SRR4302533.3783332/1‑94 (MQ=60)
                                                               AAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                >  SRR4302533.3832783/1‑94 (MQ=60)
                                                               AAACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTT                                >  SRR4302533.534741/1‑94 (MQ=60)
                                                                AACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTG                               >  SRR4302533.4986834/1‑94 (MQ=60)
                                                                AACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTG                               <  SRR4302533.2977541/94‑1 (MQ=60)
                                                                AACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTG                               <  SRR4302533.1680767/94‑1 (MQ=60)
                                                                 ACGTGATCCACCGCGCGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGG                              <  SRR4302533.4509691/94‑1 (MQ=60)
                                                                 ACGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGG                              <  SRR4302533.1741438/94‑1 (MQ=60)
                                                                  CGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGA                             >  SRR4302533.4447729/1‑94 (MQ=60)
                                                                  CGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGA                             >  SRR4302533.2389959/1‑94 (MQ=60)
                                                                  CGTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGA                             <  SRR4302533.3572076/94‑1 (MQ=60)
                                                                   GTGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGAT                            <  SRR4302533.4849666/94‑1 (MQ=60)
                                                                    TGATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATC                           >  SRR4302533.4549407/1‑94 (MQ=60)
                                                                     GATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCC                          >  SRR4302533.5279146/1‑94 (MQ=60)
                                                                     GATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCC                          <  SRR4302533.4252605/94‑1 (MQ=60)
                                                                     GATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCC                          >  SRR4302533.5014417/1‑94 (MQ=60)
                                                                      ATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCT                         >  SRR4302533.5685578/1‑94 (MQ=60)
                                                                      ATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCT                         >  SRR4302533.3305241/1‑94 (MQ=60)
                                                                      ATCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCT                         <  SRR4302533.3904615/94‑1 (MQ=60)
                                                                       TCCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTT                        >  SRR4302533.5411893/1‑94 (MQ=60)
                                                                        CCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTGCCCTTGAAGAAAAGCCGATAATTAGCGACGACGTTCGGGGGGTGGATCCGTA                       >  SRR4302533.1141513/1‑94 (MQ=60)
                                                                        CCACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTA                       >  SRR4302533.6346119/1‑94 (MQ=60)
                                                                         CACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAT                      >  SRR4302533.1387695/1‑94 (MQ=60)
                                                                         CACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAT                      <  SRR4302533.1844088/94‑1 (MQ=60)
                                                                         CACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAT                      <  SRR4302533.1782796/94‑1 (MQ=60)
                                                                         CACCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTAT                      <  SRR4302533.4954636/94‑1 (MQ=60)
                                                                           CCGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGC                    >  SRR4302533.2642794/1‑94 (MQ=60)
                                                                            CGCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCT                   >  SRR4302533.4861526/1‑94 (MQ=60)
                                                                             GCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTC                  >  SRR4302533.1070816/1‑94 (MQ=60)
                                                                             GCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTC                  <  SRR4302533.652414/94‑1 (MQ=60)
                                                                             GCACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATCAGCGACGAATTTCGGAGGTTGGATCCTTATGCTC                  <  SRR4302533.6181471/94‑1 (MQ=60)
                                                                              CACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAACGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCA                 >  SRR4302533.4464652/1‑94 (MQ=60)
                                                                              CACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCA                 <  SRR4302533.4927253/94‑1 (MQ=60)
                                                                               ACGCTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAA                >  SRR4302533.5052371/1‑94 (MQ=60)
                                                                                  CTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCA             <  SRR4302533.910361/94‑1 (MQ=60)
                                                                                  CTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCA             >  SRR4302533.3165542/1‑94 (MQ=60)
                                                                                  CTTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCA             <  SRR4302533.4262773/94‑1 (MQ=60)
                                                                                   TTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAG            <  SRR4302533.5745818/94‑1 (MQ=60)
                                                                                   TTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAG            <  SRR4302533.2190881/94‑1 (MQ=60)
                                                                                   TTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAG            <  SRR4302533.276082/94‑1 (MQ=60)
                                                                                   TTTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAG            <  SRR4302533.3307703/94‑1 (MQ=60)
                                                                                    TTGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGA           <  SRR4302533.2822647/94‑1 (MQ=60)
                                                                                     TGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAA          >  SRR4302533.908991/1‑94 (MQ=60)
                                                                                     TGTCGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAA          <  SRR4302533.264879/94‑1 (MQ=60)
                                                                                        CGCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAAT       <  SRR4302533.3629545/94‑1 (MQ=60)
                                                                                         GCCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATT      <  SRR4302533.1670676/94‑1 (MQ=60)
                                                                                          CCCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTC     >  SRR4302533.5552796/1‑94 (MQ=60)
                                                                                           CCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCA    <  SRR4302533.6014220/94‑1 (MQ=60)
                                                                                           CCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCA    >  SRR4302533.5724681/1‑94 (MQ=60)
                                                                                           CCGCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCA    <  SRR4302533.4150608/94‑1 (MQ=60)
                                                                                             GCCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAA  <  SRR4302533.2486990/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACCAAATCGGTATTTTTATAAATTCCATTGATATTAGTGCGTTATATTTTTTGACAGGTTAATAAACGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCGATAATTAGCGACGAATTTCGGAGGTTGGATCCTTATGCTCAATCAGAAAATTCAAA  >  NC_000913/3667057‑3667243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑