Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,686,775	+T	intergenic (‑23/+9)	b3530 ← / ← yhjM	putative oxidoreductase subunit/putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,775	1	.	T	99.6%	861.3 / ‑6.3	236	intergenic (‑23/+9)	b3530/yhjM	putative oxidoreductase subunit/putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (0/0); major base T (92/143); minor base A (1/0); total (93/143)
Fisher's exact test for biased strand distribution p-value = 3.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 262 total aligned reads displayed.

TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686683‑3686861

tCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCg                                                                                         <  1:5340582/93‑1 (MQ=255)

tCGCGTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCg                                                                                         <  1:1239296/93‑1 (MQ=255)

 cGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgt                                                                                        <  1:3970378/93‑2 (MQ=255)

  gCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGGCAGACCGAGGGAAAGCgtg                                                                                       >  1:257688/1‑91 (MQ=255)

   cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtga                                                                                      >  1:1134912/1‑90 (MQ=255)

   cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtga                                                                                      >  2:5900262/1‑90 (MQ=255)

   cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtga                                                                                      <  2:551917/93‑4 (MQ=255)

    ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCgtgaa                                                                                     >  1:5494803/1‑89 (MQ=255)

      cGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAata                                                                                   <  2:1577582/93‑1 (MQ=255)

          tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGtt                                                                               >  2:5186093/1‑93 (MQ=255)

          tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGtt                                                                               >  2:2744169/1‑93 (MQ=255)

          tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGtt                                                                               <  2:4971318/93‑1 (MQ=255)

          tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGACGGAAAGCGTGAATATGtt                                                                               >  1:4496409/1‑93 (MQ=255)

           aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGttt                                                                              >  1:2480797/1‑93 (MQ=255)

           aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGttt                                                                              >  2:4723044/1‑93 (MQ=255)

           aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGttt                                                                              <  1:3326580/93‑1 (MQ=255)

            aCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTa                                                                             >  2:2651748/1‑93 (MQ=255)

             ccGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAg                                                                            <  1:1030359/93‑1 (MQ=255)

             ccGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAg                                                                            >  1:792916/1‑93 (MQ=255)

              cGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgt                                                                           <  1:2498316/93‑1 (MQ=255)

              cGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgt                                                                           <  2:787086/93‑1 (MQ=255)

                aaCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt                                                                         >  1:4027200/1‑93 (MQ=255)

                aaCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAgtgt                                                                         >  1:1223356/1‑93 (MQ=255)

                   ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGaa                                                                      >  1:5884295/1‑93 (MQ=255)

                   ctGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGaa                                                                      <  1:5181957/92‑1 (MQ=255)

                    tGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAt                                                                     <  1:1403950/93‑1 (MQ=255)

                     gCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAtt                                                                    <  2:3998477/93‑1 (MQ=255)

                      cTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAttt                                                                   >  2:3428764/1‑93 (MQ=255)

                      cTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAttt                                                                   <  1:3606638/93‑1 (MQ=255)

                      cTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAttt                                                                   >  1:3785864/1‑93 (MQ=255)

                      cTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAttt                                                                   <  1:1887806/93‑1 (MQ=255)

                       tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTg                                                                  <  2:3112797/93‑1 (MQ=255)

                       tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTg                                                                  <  2:4126308/93‑1 (MQ=255)

                       cccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTg                                                                  <  1:242445/92‑1 (MQ=255)

                        ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTgc                                                                 <  1:5192651/93‑1 (MQ=255)

                        ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTgc                                                                 >  2:4458254/1‑93 (MQ=255)

                           gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCa                                                              <  1:3353997/93‑1 (MQ=255)

                           gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCa                                                              >  2:5607609/1‑93 (MQ=255)

                           gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCa                                                              <  2:3565817/93‑1 (MQ=255)

                            cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAt                                                             <  2:6058910/93‑1 (MQ=255)

                             aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAtt                                                            >  2:575506/1‑93 (MQ=255)

                             aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGAGTGAATTTGCGCttt                                                            >  1:4994718/1‑93 (MQ=255)

                              aCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTc                                                           >  1:6056299/1‑93 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  2:5136976/93‑1 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  1:2734725/93‑1 (MQ=255)

                                tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt                                                         <  2:5245541/93‑1 (MQ=255)

                                tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt                                                         <  2:2421703/93‑1 (MQ=255)

                                tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCt                                                         <  2:1335971/93‑1 (MQ=255)

                                  ttGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg                                                       >  1:3027924/1‑93 (MQ=255)

                                    gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGCTTAGTGTGAATTTGCGCATTCCTGGcc                                                     >  1:4884555/1‑93 (MQ=255)

                                      tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc                                                   >  2:2550480/1‑93 (MQ=255)

                                      tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc                                                   >  2:2033654/1‑93 (MQ=255)

                                       gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                                  <  2:932427/93‑1 (MQ=255)

                                       gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                                  >  1:202774/1‑93 (MQ=255)

                                       gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                                  <  2:5367710/93‑1 (MQ=255)

                                        aGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAg                                                 >  1:3192801/1‑93 (MQ=255)

                                           ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCa                                              <  2:4314985/93‑1 (MQ=255)

                                            gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTACTGGCCCCAGTCAg                                             >  2:1068074/1‑93 (MQ=255)

                                              tGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGt                                           <  1:5555944/93‑1 (MQ=255)

                                               ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGCTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta                                          >  2:4014506/1‑93 (MQ=255)

                                                gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa                                         <  1:2962080/93‑1 (MQ=255)

                                                  gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata                                       >  1:753748/1‑93 (MQ=255)

                                                  gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata                                       <  1:6141396/93‑1 (MQ=255)

                                                  gctgcCGCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATGCCTGGCCCCAGTCAGGtaata                                       <  2:1419409/93‑1 (MQ=255)

                                                   ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa                                      >  1:56808/1‑93 (MQ=255)

                                                   ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa                                      <  2:373624/93‑1 (MQ=255)

                                                      ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc                                   <  1:1380172/93‑1 (MQ=255)

                                                      ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc                                   <  1:4641011/93‑1 (MQ=255)

                                                       cTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCa                                  <  1:3263195/93‑1 (MQ=255)

                                                        tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc                                 >  1:5091793/1‑93 (MQ=255)

                                                          gACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt                               <  1:407346/93‑1 (MQ=255)

                                                           aCCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt                              <  1:1533400/93‑1 (MQ=255)

                                                           aCCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCACTCCTGGCCCCAGTCAGGTTATAACTCACCtt                              <  2:5042254/93‑1 (MQ=255)

                                                             cATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtttt                            <  1:306117/93‑1 (MQ=255)

                                                               tCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          <  1:6260819/93‑1 (MQ=255)

                                                               tCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          <  1:2320751/93‑1 (MQ=255)

                                                                 ggCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg                        >  2:3783260/1‑93 (MQ=255)

                                                                 ggCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg                        <  2:2142301/93‑1 (MQ=255)

                                                                   gaTGACGCCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgag                      <  2:745771/92‑1 (MQ=255)

                                                                     tGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa                    >  2:3349051/1‑93 (MQ=255)

                                                                      gACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAg                   <  1:6085823/93‑1 (MQ=255)

                                                                       gcGGCCAGACCGAGGGATAGCGTAAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGc                  <  2:1404812/92‑1 (MQ=255)

                                                                       aCGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGc                  <  1:2374251/93‑1 (MQ=255)

                                                                       aCGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGc                  <  1:3101165/93‑1 (MQ=255)

                                                                             aGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAc            <  1:4153/93‑1 (MQ=255)

                                                                             aGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAc            <  1:4270495/93‑1 (MQ=255)

                                                                              gACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           >  2:435744/1‑93 (MQ=255)

                                                                                ccGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgt         >  2:165962/1‑93 (MQ=255)

                                                                                ccGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgt         >  2:2358361/1‑93 (MQ=255)

                                                                                ccGAGAGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgt         <  1:5723489/93‑1 (MQ=255)

                                                                                 cGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtg        <  1:3650750/93‑1 (MQ=255)

                                                                                   aGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      <  2:4608377/93‑1 (MQ=255)

                                                                                   aGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      >  1:3416367/1‑93 (MQ=255)

                                                                                    gggAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTg     <  2:2867932/93‑1 (MQ=255)

                                                                                     ggAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGa    <  2:2012572/93‑1 (MQ=255)

                                                                                     ggAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGa    <  1:1628056/93‑1 (MQ=255)

                                                                                      gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAt   <  2:3037573/93‑1 (MQ=255)

                                                                                      gAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAt   >  1:2171308/1‑93 (MQ=255)

                                                                                       aaaGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATc  >  1:1667884/1‑93 (MQ=255)

                                                                                       aaaGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATc  >  1:4507340/1‑93 (MQ=255)

TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686683‑3686861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG                                                                                                >  SRR4302533.436340/1‑94 (MQ=60)

 TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑T                                                                                              <  SRR4302533.4056298/94‑1 (MQ=60)

 TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑T                                                                                              <  SRR4302533.5372608/94‑1 (MQ=60)

 TCGCGTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑T                                                                                              <  SRR4302533.1246702/94‑1 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGGCAGACCGAGGGAAAGCGtg                                                                                              >  SRR4302533.259254/1‑92 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtg                                                                                              <  SRR4302533.3994555/94‑3 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtg                                                                                              >  SRR4302533.1412057/1‑92 (MQ=60)

   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGtga                                                                                             >  SRR4302533.1141661/1‑91 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAA                                                                                            >  SRR4302533.5527950/1‑94 (MQ=60)

     TTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAAT                                                                                           <  SRR4302533.1139891/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCTTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATA                                                                                          <  SRR4302533.4470144/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATA                                                                                          <  SRR4302533.4889132/94‑1 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGT                                                                                       >  SRR4302533.3046926/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTT                                                                                      >  SRR4302533.674401/1‑94 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGACGGAAAGCGTGAATATGTT                                                                                      >  SRR4302533.4523638/1‑94 (MQ=60)

           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTT                                                                                     >  SRR4302533.2496096/1‑94 (MQ=60)

           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTT                                                                                     <  SRR4302533.2564213/94‑1 (MQ=60)

            AACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTA                                                                                    <  SRR4302533.40903/94‑1 (MQ=60)

            AACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTA                                                                                    <  SRR4302533.3347135/94‑1 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAG                                                                                   >  SRR4302533.797583/1‑94 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAG                                                                                   <  SRR4302533.2745799/94‑1 (MQ=60)

              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGT                                                                                  <  SRR4302533.1036419/94‑1 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 >  SRR4302533.3421582/1‑94 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTG                                                                                 <  SRR4302533.2513728/94‑1 (MQ=60)

                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGT                                                                                >  SRR4302533.1230693/1‑94 (MQ=60)

                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGT                                                                                >  SRR4302533.4051716/1‑94 (MQ=60)

                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGT                                                                                <  SRR4302533.299214/94‑1 (MQ=60)

                 AACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTG                                                                               >  SRR4302533.5513836/1‑94 (MQ=60)

                   CTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAA                                                                             >  SRR4302533.5919854/1‑94 (MQ=60)

                    TTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAT                                                                            <  SRR4302533.2210710/94‑1 (MQ=60)

                    CTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAAT                                                                            <  SRR4302533.5213245/94‑1 (MQ=60)

                     TGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATT                                                                           >  SRR4302533.3363751/1‑94 (MQ=60)

                     TGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATT                                                                           <  SRR4302533.1412448/94‑1 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTT                                                                          >  SRR4302533.3809043/1‑94 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302533.3269259/94‑1 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302533.3628842/94‑1 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302533.776723/94‑1 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302533.1899335/94‑1 (MQ=60)

                        CCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGC                                                                        <  SRR4302533.243947/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302533.5547255/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302533.5223973/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       >  SRR4302533.4576671/1‑94 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGCAAAGCGTGAATATGTTTAGTGTGAATTTGCG                                                                       <  SRR4302533.336025/94‑1 (MQ=60)

                          CAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGC                                                                      <  SRR4302533.1184777/94‑1 (MQ=60)

                           AGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCA                                                                     <  SRR4302533.87112/94‑1 (MQ=60)

                            GCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAT                                                                    <  SRR4302533.6111523/94‑1 (MQ=60)

                            GCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAT                                                                    <  SRR4302533.3374723/94‑1 (MQ=60)

                            GCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCAT                                                                    >  SRR4302533.5563609/1‑94 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   >  SRR4302533.4786042/1‑94 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGAGTGAATTTGCGCTTT                                                                   >  SRR4302533.5024997/1‑94 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302533.6092972/1‑94 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  <  SRR4302533.3308273/94‑1 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  <  SRR4302533.2470940/94‑1 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 >  SRR4302533.4757605/1‑94 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302533.1978743/94‑1 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302533.4153256/94‑1 (MQ=60)

                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302533.3178814/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                >  SRR4302533.6232947/1‑94 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302533.2751500/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302533.784006/94‑1 (MQ=60)

                                 TGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTG                                                               >  SRR4302533.3706525/1‑94 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302533.5708279/94‑1 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              >  SRR4302533.3046455/1‑94 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGCGAATATGTTTAGTGTGAATTTGCGCATTCCTGC                                                              >  SRR4302533.2548371/1‑94 (MQ=60)

                                   TTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGC                                                             <  SRR4302533.1245647/94‑1 (MQ=60)

                                    TGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCC                                                            <  SRR4302533.1493420/94‑1 (MQ=60)

                                    TGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGCTTAGTGTGAATTTGCGCATTCCTGGCC                                                            >  SRR4302533.4914148/1‑94 (MQ=60)

                                       TGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCA                                                         >  SRR4302533.5704672/1‑94 (MQ=60)

                                       TGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCA                                                         >  SRR4302533.204063/1‑94 (MQ=60)

                                       TGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCA                                                         >  SRR4302533.4514279/1‑94 (MQ=60)

                                       TGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCA                                                         >  SRR4302533.5182029/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        >  SRR4302533.3212440/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        <  SRR4302533.5971839/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302533.4245231/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302533.1162065/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302533.2999782/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302533.1352284/94‑1 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302533.5937988/94‑1 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302533.6145667/94‑1 (MQ=60)

                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGGCCGAGGGCAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAAGTA                                                 >  SRR4302533.4552821/1‑94 (MQ=60)

                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302533.5748917/94‑1 (MQ=60)

                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302533.5589519/94‑1 (MQ=60)

                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                >  SRR4302533.4042499/1‑94 (MQ=60)

                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               >  SRR4302533.2353612/1‑94 (MQ=60)

                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               <  SRR4302533.3048280/94‑1 (MQ=60)

                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               <  SRR4302533.2980188/94‑1 (MQ=60)

                                                  TGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATA                                              <  SRR4302533.3081165/94‑1 (MQ=60)

                                                  TGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATA                                              >  SRR4302533.758186/1‑94 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             <  SRR4302533.5076267/94‑1 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             <  SRR4302533.6178384/94‑1 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             >  SRR4302533.57184/1‑94 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             <  SRR4302533.4983929/94‑1 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            <  SRR4302533.1991883/94‑1 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            <  SRR4302533.5084956/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302533.2650225/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302533.1388484/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302533.4669130/94‑1 (MQ=60)

                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         >  SRR4302533.4531079/1‑94 (MQ=60)

                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        <  SRR4302533.3283325/94‑1 (MQ=60)

                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        >  SRR4302533.5122553/1‑94 (MQ=60)

                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGAGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        >  SRR4302533.5169990/1‑94 (MQ=60)

                                                         TCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACC                                       <  SRR4302533.5355144/94‑1 (MQ=60)

                                                         TCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACC                                       <  SRR4302533.5881480/94‑1 (MQ=60)

                                                         TCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACC                                       >  SRR4302533.615816/1‑94 (MQ=60)

                                                           GACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTT                                     >  SRR4302533.3658703/1‑94 (MQ=60)

                                                           GACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTT                                     <  SRR4302533.409795/94‑1 (MQ=60)

                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    <  SRR4302533.1542610/94‑1 (MQ=60)

                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    <  SRR4302533.3537363/94‑1 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   >  SRR4302533.5213561/1‑94 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   >  SRR4302533.1809863/1‑94 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  <  SRR4302533.307987/94‑1 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  >  SRR4302533.1400151/1‑94 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  >  SRR4302533.2144393/1‑94 (MQ=60)

                                                                TCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302533.6298250/94‑1 (MQ=60)

                                                                TCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302533.2335116/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302533.1810695/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               >  SRR4302533.3979809/1‑94 (MQ=60)

                                                                   GCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAG                             <  SRR4302533.4165976/94‑1 (MQ=60)

                                                                   GCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAG                             <  SRR4302533.2378774/94‑1 (MQ=60)

                                                                    CATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGA                            <  SRR4302533.3156211/94‑1 (MQ=60)

                                                                     ATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           <  SRR4302533.2785629/94‑1 (MQ=60)

                                                                     ATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           >  SRR4302533.66162/1‑94 (MQ=60)

                                                                     ATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           >  SRR4302533.1635821/1‑94 (MQ=60)

                                                                     ATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           <  SRR4302533.3970056/94‑1 (MQ=60)

                                                                      TGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          >  SRR4302533.1612608/1‑94 (MQ=60)

                                                                       GACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGC                         <  SRR4302533.6122616/94‑1 (MQ=60)

                                                                        ACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCG                        <  SRR4302533.2388944/94‑1 (MQ=60)

                                                                        ACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCG                        <  SRR4302533.2471180/94‑1 (MQ=60)

                                                                        ACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCG                        <  SRR4302533.3120228/94‑1 (MQ=60)

                                                                        ACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCG                        <  SRR4302533.2499375/94‑1 (MQ=60)

                                                                             CAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   >  SRR4302533.5788917/1‑94 (MQ=60)

                                                                             CAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCACTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302533.2396528/94‑1 (MQ=60)

                                                                              AGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  >  SRR4302533.3283540/1‑94 (MQ=60)

                                                                              AGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  <  SRR4302533.4188/94‑1 (MQ=60)

                                                                              AGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  <  SRR4302533.4296201/94‑1 (MQ=60)

                                                                               GACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGG                 <  SRR4302533.5364240/94‑1 (MQ=60)

                                                                                 CCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTG               <  SRR4302533.4593424/94‑1 (MQ=60)

                                                                                 CCGAGAGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTG               <  SRR4302533.5758042/94‑1 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302533.2217468/1‑94 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              <  SRR4302533.3673197/94‑1 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302533.3576561/1‑94 (MQ=60)

                                                                                  CGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              <  SRR4302533.1354846/94‑1 (MQ=60)

                                                                                   GAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302533.3437474/1‑94 (MQ=60)

                                                                                     GGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGA           <  SRR4302533.1676820/94‑1 (MQ=60)

                                                                                      GGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          <  SRR4302533.3802498/94‑1 (MQ=60)

                                                                                      GGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          >  SRR4302533.2184662/1‑94 (MQ=60)

                                                                                      GGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          <  SRR4302533.1637915/94‑1 (MQ=60)

                                                                                       GAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         >  SRR4302533.4534638/1‑94 (MQ=60)

                                                                                       GAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         >  SRR4302533.1677960/1‑94 (MQ=60)

                                                                                         AAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       <  SRR4302533.5174129/94‑1 (MQ=60)

                                                                                          AGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      <  SRR4302533.3513768/94‑1 (MQ=60)

                                                                                          AGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302533.23176/1‑94 (MQ=60)

                                                                                           GCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAG     <  SRR4302533.3475591/94‑1 (MQ=60)

                                                                                           GCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAG     <  SRR4302533.4675726/94‑1 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302533.1545824/1‑94 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302533.4789969/1‑94 (MQ=60)

                                                                                             T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302533.2849502/1‑94 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCG‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`