Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,739,740 A→G M141T (ATG→ACG)  yiaJ ← putative regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,739,7400AG99.6% 791.0 / ‑5.1 248M141T (ATG→ACG) yiaJputative regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (111/136);  total (112/136)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 265 total aligned reads displayed.

CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTC  >  NC_000913/3739648‑3739829
                                                                                            |                                                                                         
  tAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTc                                                                                         >  1:929615/1‑93 (MQ=255)
   aGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTcc                                                                                        <  2:2302045/93‑1 (MQ=255)
    gTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTccc                                                                                       >  1:2101982/1‑93 (MQ=255)
       cGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGt                                                                                    >  1:4464097/1‑93 (MQ=255)
       cGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGt                                                                                    <  1:5217055/93‑1 (MQ=255)
        gggTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGtt                                                                                   <  1:5923572/93‑1 (MQ=255)
         ggTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtg                                                                                  >  2:1468169/1‑93 (MQ=255)
          gTGACTAAACGCCATGCAGATCTTGTCTGTTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATATGCACGGGTTCGCAGCGTCCCGGTtgt                                                                                 <  2:3562083/93‑1 (MQ=255)
          gTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgt                                                                                 <  1:2900503/93‑1 (MQ=255)
           ttaCCAAACGCATTGTAGACCTTGCCCATTGCGGAGCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                <  2:3907640/91‑1 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                >  2:3419366/1‑93 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                >  2:2972803/1‑93 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                >  1:396486/1‑93 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                >  1:2794125/1‑93 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                <  1:4805569/93‑1 (MQ=255)
           tGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTtgtg                                                                                <  2:2127625/93‑1 (MQ=255)
            gACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTgg                                                                               <  2:1181996/93‑1 (MQ=255)
             aCCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTggg                                                                              >  1:4188578/1‑93 (MQ=255)
             aCCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTggg                                                                              <  2:2866491/93‑1 (MQ=255)
               cAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGtt                                                                            <  1:1116911/93‑1 (MQ=255)
                aaaCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTc                                                                           >  2:5353912/1‑93 (MQ=255)
                 aaCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTcc                                                                          >  1:2516809/1‑93 (MQ=255)
                 aaCGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTcc                                                                          <  1:258398/93‑1 (MQ=255)
                    gCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGc                                                                       <  1:1345424/93‑1 (MQ=255)
                    gCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGc                                                                       <  2:5380327/93‑1 (MQ=255)
                     ccATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCt                                                                      >  1:3712331/1‑93 (MQ=255)
                       aTGTAGATCTTGCCCATTGCGGATCAGTAGAGCGGCATATGCGGGCCAATATAGGCACGGGGTCGCAGCGTCCCGGTTGTGGGTTCCAGCTta                                                                    >  1:6301894/1‑93 (MQ=255)
                       aTGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCTGGTTGTGGGTTCCAGCTta                                                                    <  1:5674406/93‑1 (MQ=255)
                       aTGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTta                                                                    >  1:2476253/1‑93 (MQ=255)
                        tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  2:3083269/1‑93 (MQ=255)
                        tGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtat                                                                   >  2:5327222/1‑93 (MQ=255)
                         gTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtata                                                                  <  1:1039515/93‑1 (MQ=255)
                         gTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTtata                                                                  <  1:4258776/93‑1 (MQ=255)
                          tAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaa                                                                 <  2:3870432/93‑1 (MQ=255)
                          tAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaa                                                                 <  2:2444901/93‑1 (MQ=255)
                          tAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaa                                                                 <  1:5287168/93‑1 (MQ=255)
                           aGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaaa                                                                <  2:1741461/93‑1 (MQ=255)
                           aGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATaaa                                                                <  1:1210791/93‑1 (MQ=255)
                             aTCTTGCCCATTGCGGAACAGTAGAGCGGCATATGGTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCCGCTTATAAATc                                                              >  2:4835654/1‑93 (MQ=255)
                             aTCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCCGCTTATAAATc                                                              >  2:1393688/1‑93 (MQ=255)
                             aTCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGATCGCAGCGTCCCGGGTGTGGGTTCCAGCATATAAATc                                                              >  2:4000455/1‑93 (MQ=255)
                              tCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCa                                                             >  1:2136512/1‑93 (MQ=255)
                              tCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCa                                                             <  1:5773789/93‑1 (MQ=255)
                               cTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaa                                                            >  1:2323447/1‑93 (MQ=255)
                               cTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaa                                                            <  2:5004496/93‑1 (MQ=255)
                                ttGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaaa                                                           >  2:4594949/1‑93 (MQ=255)
                                ttGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCaaa                                                           <  1:5041210/93‑1 (MQ=255)
                                  gCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAt                                                         <  2:2361103/93‑1 (MQ=255)
                                  gCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAt                                                         <  1:1021828/93‑1 (MQ=255)
                                    ccATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAg                                                       <  1:3801356/93‑1 (MQ=255)
                                     cATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGc                                                      <  1:2982006/93‑1 (MQ=255)
                                      aTTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCg                                                     <  1:2304058/93‑1 (MQ=255)
                                       ttGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGt                                                    >  1:989097/1‑93 (MQ=255)
                                          cGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGAt                                                 <  2:1601965/93‑1 (MQ=255)
                                          cGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCAGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGAt                                                 <  2:4726949/93‑1 (MQ=255)
                                           ggAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATc                                                >  2:3640278/1‑93 (MQ=255)
                                           ggAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATc                                                >  2:3741450/1‑93 (MQ=255)
                                            gAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCg                                               >  1:2055844/1‑93 (MQ=255)
                                            gAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCg                                               >  2:1975745/1‑93 (MQ=255)
                                             aaCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGt                                              <  2:2218258/93‑1 (MQ=255)
                                              aCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTc                                             >  1:3336959/1‑93 (MQ=255)
                                                aGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCtt                                           <  2:2686131/93‑1 (MQ=255)
                                                 gTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTc                                          <  1:4816698/93‑1 (MQ=255)
                                                 gTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTc                                          <  2:1648909/93‑1 (MQ=255)
                                                   agagCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTcgc                                        <  2:4294969/93‑1 (MQ=255)
                                                        ggCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTg                                   <  2:5868923/93‑1 (MQ=255)
                                                          cATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGga                                 <  2:1758066/93‑1 (MQ=255)
                                                          cATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGATggg                                 >  1:1495317/1‑92 (MQ=255)
                                                            tatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGgaga                               >  1:4032242/1‑93 (MQ=255)
                                                            tatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGgaga                               <  1:2711150/93‑1 (MQ=255)
                                                            tatGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGgaga                               >  1:1431925/1‑93 (MQ=255)
                                                             atGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGaa                              >  2:6019192/1‑93 (MQ=255)
                                                              tGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAg                             >  2:4431826/1‑93 (MQ=255)
                                                                 tGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTa                          >  1:1656123/1‑93 (MQ=255)
                                                                  ggCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTaa                         >  2:3819622/1‑93 (MQ=255)
                                                                   gCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAAt                        <  1:75741/93‑1 (MQ=255)
                                                                    ccAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATg                       <  1:4355636/93‑1 (MQ=255)
                                                                    ccAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATg                       >  1:2203743/1‑93 (MQ=255)
                                                                     cAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGTAGTTAATgg                      >  1:4563292/1‑93 (MQ=255)
                                                                     cAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATgg                      >  1:6081928/1‑93 (MQ=255)
                                                                      aaTATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGt                     <  2:2842863/93‑1 (MQ=255)
                                                                      aaTATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGt                     >  1:3476099/1‑93 (MQ=255)
                                                                         ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTTATGGTTTc                  <  2:6124934/93‑1 (MQ=255)
                                                                         ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTc                  <  2:6286770/93‑1 (MQ=255)
                                                                         ataGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTc                  >  1:6130196/1‑93 (MQ=255)
                                                                          taGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCa                 <  1:1746738/93‑1 (MQ=255)
                                                                          taGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCa                 >  1:5611674/1‑93 (MQ=255)
                                                                            ggCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCAcc               <  1:5398021/93‑1 (MQ=255)
                                                                             gCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCa              >  1:2281668/1‑93 (MQ=255)
                                                                             gCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCa              <  2:2791382/93‑1 (MQ=255)
                                                                              cACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAg             <  2:1837833/93‑1 (MQ=255)
                                                                               gcGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGt            <  2:4493680/92‑1 (MQ=255)
                                                                                cGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTg           <  1:1590210/93‑1 (MQ=255)
                                                                                 gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          <  1:5903315/93‑1 (MQ=255)
                                                                                 gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          >  2:5721863/1‑93 (MQ=255)
                                                                                 gggTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTgg          >  1:4112061/1‑93 (MQ=255)
                                                                                     tCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGAt      <  2:4829528/93‑1 (MQ=255)
                                                                                       gcagcgTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGt    <  2:1269273/93‑1 (MQ=255)
                                                                                       gcagcgTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGt    <  2:707925/93‑1 (MQ=255)
                                                                                        cagcgTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGtt   <  1:473711/93‑1 (MQ=255)
                                                                                            |                                                                                         
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTC  >  NC_000913/3739648‑3739829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTG  >  NC_000913/3739648‑3739833
                                                                                            |                                                                                             
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGT                                                                                              <  SRR4302533.6145242/94‑1 (MQ=60)
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGT                                                                                              >  SRR4302533.3773960/1‑94 (MQ=60)
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGT                                                                                              >  SRR4302533.6322265/1‑94 (MQ=60)
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGT                                                                                              >  SRR4302533.1698133/1‑94 (MQ=60)
 GTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTC                                                                                             >  SRR4302533.2547617/1‑94 (MQ=60)
 GTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTC                                                                                             >  SRR4302533.935073/1‑94 (MQ=60)
  TAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCC                                                                                            <  SRR4302533.4321196/94‑1 (MQ=60)
   AGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCC                                                                                           <  SRR4302533.2383047/94‑1 (MQ=60)
   AGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCC                                                                                           >  SRR4302533.2114944/1‑94 (MQ=60)
   AGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCC                                                                                           <  SRR4302533.2608192/94‑1 (MQ=60)
    GTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCG                                                                                          <  SRR4302533.4496197/94‑1 (MQ=60)
      CCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGT                                                                                        >  SRR4302533.4491097/1‑94 (MQ=60)
       CGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTT                                                                                       <  SRR4302533.5248463/94‑1 (MQ=60)
       CGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTT                                                                                       <  SRR4302533.1340554/94‑1 (MQ=60)
        GGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTG                                                                                      <  SRR4302533.5959378/94‑1 (MQ=60)
         GGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCTTATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGT                                                                                     <  SRR4302533.3410725/94‑1 (MQ=60)
          GTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTG                                                                                    >  SRR4302533.2811312/1‑94 (MQ=60)
          GTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTG                                                                                    >  SRR4302533.398886/1‑94 (MQ=60)
          GTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTG                                                                                    <  SRR4302533.2918322/94‑1 (MQ=60)
           TGACCAAACGCCATGTAGATCTTGCCCATTGCGGAGCAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGG                                                                                   <  SRR4302533.4816456/94‑1 (MQ=60)
           TGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGG                                                                                   >  SRR4302533.4711736/1‑94 (MQ=60)
           TGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGG                                                                                   <  SRR4302533.4834666/94‑1 (MQ=60)
            GACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGG                                                                                  >  SRR4302533.4213940/1‑94 (MQ=60)
              CCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTT                                                                                <  SRR4302533.2160141/94‑1 (MQ=60)
               CAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTC                                                                               <  SRR4302533.4767650/94‑1 (MQ=60)
               CAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTC                                                                               <  SRR4302533.1123539/94‑1 (MQ=60)
               CAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTC                                                                               <  SRR4302533.1659490/94‑1 (MQ=60)
                AAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCC                                                                              >  SRR4302533.2532316/1‑94 (MQ=60)
                 AACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCA                                                                             <  SRR4302533.259971/94‑1 (MQ=60)
                  ACGCCATGTAGATCTTGCTCATTGCGGAACATTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAG                                                                            <  SRR4302533.98586/94‑1 (MQ=60)
                  ACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAG                                                                            <  SRR4302533.5523198/94‑1 (MQ=60)
                    GCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCT                                                                          >  SRR4302533.3202972/1‑94 (MQ=60)
                    GCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCT                                                                          >  SRR4302533.3735127/1‑94 (MQ=60)
                    GCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCT                                                                          <  SRR4302533.1353520/94‑1 (MQ=60)
                      CATGTAGATCTTGCCCATTGCGGATCAGTAGAGCGGCATATGCGGGCCAATATAGGCACGGGGTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTA                                                                        >  SRR4302533.6339539/1‑94 (MQ=60)
                      CATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTA                                                                        >  SRR4302533.4312195/1‑94 (MQ=60)
                      CATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTA                                                                        >  SRR4302533.2491526/1‑94 (MQ=60)
                       ATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCTGGTTGTGGGTTCCAGCTTAT                                                                       <  SRR4302533.5708675/94‑1 (MQ=60)
                       ATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGGTCCAGCTTAT                                                                       >  SRR4302533.4445633/1‑94 (MQ=60)
                        TGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCCGCTTATA                                                                      >  SRR4302533.1523853/1‑94 (MQ=60)
                         GTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAA                                                                     <  SRR4302533.1045625/94‑1 (MQ=60)
                         GTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAA                                                                     <  SRR4302533.2985126/94‑1 (MQ=60)
                         GTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAA                                                                     <  SRR4302533.4284444/94‑1 (MQ=60)
                         GTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAA                                                                     <  SRR4302533.1380466/94‑1 (MQ=60)
                          TAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAA                                                                    <  SRR4302533.6034549/94‑1 (MQ=60)
                          TAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAA                                                                    <  SRR4302533.5318836/94‑1 (MQ=60)
                          TAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAA                                                                    <  SRR4302533.4153168/94‑1 (MQ=60)
                           AGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAAT                                                                   <  SRR4302533.1218060/94‑1 (MQ=60)
                            GATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATC                                                                  <  SRR4302533.2809884/94‑1 (MQ=60)
                             ATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCA                                                                 >  SRR4302533.2149686/1‑94 (MQ=60)
                             ATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCA                                                                 <  SRR4302533.2116941/94‑1 (MQ=60)
                              TCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAA                                                                >  SRR4302533.2337828/1‑94 (MQ=60)
                              TCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAA                                                                <  SRR4302533.5808660/94‑1 (MQ=60)
                               CTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAA                                                               >  SRR4302533.568852/1‑94 (MQ=60)
                                TTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCCGCTTATAAATCAAAA                                                              >  SRR4302533.4202747/1‑94 (MQ=60)
                                TTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAA                                                              <  SRR4302533.5071721/94‑1 (MQ=60)
                                 TGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAAT                                                             >  SRR4302533.4094464/1‑94 (MQ=60)
                                  GCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATA                                                            <  SRR4302533.1027831/94‑1 (MQ=60)
                                  GCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATA                                                            >  SRR4302533.2717928/1‑94 (MQ=60)
                                   CCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAG                                                           >  SRR4302533.4961906/1‑94 (MQ=60)
                                   CCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAG                                                           <  SRR4302533.6162061/94‑1 (MQ=60)
                                   CCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAG                                                           <  SRR4302533.740496/94‑1 (MQ=60)
                                   CCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGCTGCGGGTTCCAGCTTATAAATCAAAATAG                                                           >  SRR4302533.5771022/1‑94 (MQ=60)
                                    CCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGC                                                          <  SRR4302533.3824620/94‑1 (MQ=60)
                                    CCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGC                                                          <  SRR4302533.1482392/94‑1 (MQ=60)
                                     CATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCG                                                         <  SRR4302533.3000247/94‑1 (MQ=60)
                                     CATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCG                                                         <  SRR4302533.233512/94‑1 (MQ=60)
                                     CATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCG                                                         <  SRR4302533.2289762/94‑1 (MQ=60)
                                      ATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGT                                                        >  SRR4302533.994883/1‑94 (MQ=60)
                                      ATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGT                                                        <  SRR4302533.739418/94‑1 (MQ=60)
                                      ATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGT                                                        <  SRR4302533.2318329/94‑1 (MQ=60)
                                        TGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGA                                                      <  SRR4302533.3398358/94‑1 (MQ=60)
                                        TGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCCGTTGTGGGGTCCAGCTTATAAATCAAAATAGCGTGA                                                      >  SRR4302533.5379637/1‑94 (MQ=60)
                                         GCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGAT                                                     <  SRR4302533.5735528/94‑1 (MQ=60)
                                           GGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCG                                                   <  SRR4302533.4393131/94‑1 (MQ=60)
                                           GGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCG                                                   >  SRR4302533.2068525/1‑94 (MQ=60)
                                            GAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGT                                                  >  SRR4302533.5368433/1‑94 (MQ=60)
                                             AACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTC                                                 >  SRR4302533.3357581/1‑94 (MQ=60)
                                             AACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTC                                                 >  SRR4302533.3262041/1‑94 (MQ=60)
                                              ACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCT                                                <  SRR4302533.1102135/94‑1 (MQ=60)
                                                AGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTC                                              <  SRR4302533.1643846/94‑1 (MQ=60)
                                                 GTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCG                                             <  SRR4302533.4845892/94‑1 (MQ=60)
                                                  TAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGC                                            <  SRR4302533.2824143/94‑1 (MQ=60)
                                                   AGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCG                                           <  SRR4302533.525456/94‑1 (MQ=60)
                                                   AGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCG                                           <  SRR4302533.2315585/94‑1 (MQ=60)
                                                    GAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGG                                          <  SRR4302533.5959182/94‑1 (MQ=60)
                                                     AGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGC                                         <  SRR4302533.2183539/94‑1 (MQ=60)
                                                      GCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCT                                        <  SRR4302533.1895566/94‑1 (MQ=60)
                                                         GCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGatggg                                     >  SRR4302533.1504327/1‑89 (MQ=60)
                                                         GCATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGA                                     >  SRR4302533.5151445/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.4265342/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.6228395/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.23287/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.1261140/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.4056788/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.4738701/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.1440576/1‑94 (MQ=60)
                                                           ATATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGCTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGA                                   >  SRR4302533.4844749/1‑94 (MQ=60)
                                                            TATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAA                                  <  SRR4302533.2727794/94‑1 (MQ=60)
                                                            TATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAA                                  >  SRR4302533.1400353/1‑94 (MQ=60)
                                                             ATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAG                                 >  SRR4302533.5676404/1‑94 (MQ=60)
                                                             ATGCTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAG                                 <  SRR4302533.4903372/94‑1 (MQ=60)
                                                                CTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGGAGTTA                              >  SRR4302533.1961388/1‑94 (MQ=60)
                                                                CTGGCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTA                              >  SRR4302533.1666146/1‑94 (MQ=60)
                                                                   GCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATG                           <  SRR4302533.76233/94‑1 (MQ=60)
                                                                   GCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATG                           >  SRR4302533.2217326/1‑94 (MQ=60)
                                                                   GCCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGACTTCGCGGCTGGAGAAGTTAATG                           <  SRR4302533.4066956/94‑1 (MQ=60)
                                                                    CCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGTAGTTAATGG                          >  SRR4302533.4590963/1‑94 (MQ=60)
                                                                    CCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGG                          >  SRR4302533.5930258/1‑94 (MQ=60)
                                                                    CCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGG                          <  SRR4302533.4381929/94‑1 (MQ=60)
                                                                    CCAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGG                          >  SRR4302533.6118705/1‑94 (MQ=60)
                                                                     CAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGT                         >  SRR4302533.3497548/1‑94 (MQ=60)
                                                                     CAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGT                         >  SRR4302533.6332755/1‑94 (MQ=60)
                                                                     CAATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGGTAATGGT                         >  SRR4302533.1029292/1‑94 (MQ=60)
                                                                      AATATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTT                        >  SRR4302533.947846/1‑94 (MQ=60)
                                                                        TATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTC                      <  SRR4302533.1135677/94‑1 (MQ=60)
                                                                        TATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTC                      >  SRR4302533.6167134/1‑94 (MQ=60)
                                                                        TATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTC                      <  SRR4302533.22906/94‑1 (MQ=60)
                                                                        TATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTC                      <  SRR4302533.2105198/94‑1 (MQ=60)
                                                                         ATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGGGTAGTTAATGGTTTCA                     >  SRR4302533.355556/1‑94 (MQ=60)
                                                                         ATAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCA                     >  SRR4302533.5645592/1‑94 (MQ=60)
                                                                          TAGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCAC                    <  SRR4302533.1757363/94‑1 (MQ=60)
                                                                           AGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACC                   >  SRR4302533.2379205/1‑94 (MQ=60)
                                                                           AGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACC                   <  SRR4302533.3838715/94‑1 (MQ=60)
                                                                           AGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACC                   >  SRR4302533.389616/1‑94 (MQ=60)
                                                                           AGGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACC                   >  SRR4302533.1538382/1‑94 (MQ=60)
                                                                            GGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCA                  <  SRR4302533.5430458/94‑1 (MQ=60)
                                                                            GGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCA                  <  SRR4302533.6001162/94‑1 (MQ=60)
                                                                            GGCACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCA                  >  SRR4302533.2295792/1‑94 (MQ=60)
                                                                              CACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGT                >  SRR4302533.594829/1‑94 (MQ=60)
                                                                              CACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGT                >  SRR4302533.2592037/1‑94 (MQ=60)
                                                                               ACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTG               >  SRR4302533.169394/1‑94 (MQ=60)
                                                                               ACGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTG               <  SRR4302533.777104/94‑1 (MQ=60)
                                                                                CGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGG              <  SRR4302533.1599829/94‑1 (MQ=60)
                                                                                CGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGG              >  SRR4302533.2110199/1‑94 (MQ=60)
                                                                                CGGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGG              >  SRR4302533.4137055/1‑94 (MQ=60)
                                                                                 GGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGC             >  SRR4302533.3778151/1‑94 (MQ=60)
                                                                                 GGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGC             <  SRR4302533.5938965/94‑1 (MQ=60)
                                                                                 GGGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGCCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGG             >  SRR4302533.1052295/1‑94 (MQ=60)
                                                                                  GGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCG            <  SRR4302533.2511319/94‑1 (MQ=60)
                                                                                  GGTTCGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCG            <  SRR4302533.5085564/94‑1 (MQ=60)
                                                                                      CGCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGT        >  SRR4302533.4045069/1‑94 (MQ=60)
                                                                                       GCAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTT       <  SRR4302533.2880385/94‑1 (MQ=60)
                                                                                        CAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTC      <  SRR4302533.476541/94‑1 (MQ=60)
                                                                                        CAGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGGTTCACCAGGGGCGGTGTTG      >  SRR4302533.3809233/1‑94 (MQ=60)
                                                                                         AGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGGAGTTAATGGTTTCACCAGTGGCGATGTTCG     >  SRR4302533.5376944/1‑94 (MQ=60)
                                                                                         AGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCA     >  SRR4302533.3978230/1‑94 (MQ=60)
                                                                                         AGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCA     >  SRR4302533.5503037/1‑94 (MQ=60)
                                                                                         AGCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCA     >  SRR4302533.6137792/1‑94 (MQ=60)
                                                                                          GCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAG    <  SRR4302533.3765467/94‑1 (MQ=60)
                                                                                          GCGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAG    <  SRR4302533.742069/94‑1 (MQ=60)
                                                                                           CGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGT   >  SRR4302533.4205270/1‑94 (MQ=60)
                                                                                           CGTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGT   <  SRR4302533.4372262/94‑1 (MQ=60)
                                                                                            GTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTG  >  SRR4302533.2589652/1‑94 (MQ=60)
                                                                                            GTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTG  >  SRR4302533.6257160/1‑94 (MQ=60)
                                                                                            GTCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTG  >  SRR4302533.159819/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGTAGTCCGGGTGACCAAACGCCATGTAGATCTTGCCCATTGCGGAACAGTAGAGCGGCATATGCTGGCCAATATAGGCACGGGTTCGCAGCATCCCGGTTGTGGGTTCCAGCTTATAAATCAAAATAGCGTGATCGTCTTCGCGGCTGGAGAAGTTAATGGTTTCACCAGTGGCGATGTTCAGTG  >  NC_000913/3739648‑3739833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑