Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,768,519	T→C	D163G (GAC→GGC)	yibH ←	putative membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,768,519	0	T	C	99.6%	781.7 / NA	245	D163G (GAC→GGC)	yibH	putative membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (107/137); minor base A (1/0); total (108/137)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

TCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT  >  NC_000913/3768429‑3768609

tCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCa                                                                                          <  1:5770659/93‑1 (MQ=255)

 cGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAc                                                                                         <  2:867262/93‑1 (MQ=255)

 cGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAc                                                                                         <  2:3157991/93‑1 (MQ=255)

   aGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGt                                                                                       >  2:5616964/1‑93 (MQ=255)

     cTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTc                                                                                     >  1:5138014/1‑93 (MQ=255)

      tGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTcc                                                                                    >  1:4563068/1‑93 (MQ=255)

       ggCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCg                                                                                   >  1:4745591/1‑93 (MQ=255)

        gCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCga                                                                                  <  2:5213814/93‑1 (MQ=255)

         ctctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCgag                                                                                 >  1:1937482/1‑93 (MQ=255)

          tctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCgaga                                                                                <  1:387532/93‑1 (MQ=255)

          tctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCgaga                                                                                >  2:4270123/1‑93 (MQ=255)

           ctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGaa                                                                               <  1:4135245/93‑1 (MQ=255)

           ctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGaa                                                                               <  1:1990611/93‑1 (MQ=255)

           ctGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGaa                                                                               >  1:2370428/1‑93 (MQ=255)

            tGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAAc                                                                              <  2:253978/93‑1 (MQ=255)

             tgATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACg                                                                             <  2:5011506/92‑1 (MQ=255)

             ggATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACg                                                                             >  2:1994231/1‑93 (MQ=255)

             ggATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACg                                                                             <  2:4953520/93‑1 (MQ=255)

              gATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACgg                                                                            >  1:2152151/1‑93 (MQ=255)

              gATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACgg                                                                            <  1:5689398/93‑1 (MQ=255)

              gATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACgg                                                                            <  2:3126185/93‑1 (MQ=255)

              gATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGCCGATGCCACGTTCCGAGAACgg                                                                            >  2:5711473/1‑93 (MQ=255)

                 cTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGAtt                                                                         >  1:5720453/1‑93 (MQ=255)

                   gcgcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCa                                                                       <  1:6019710/93‑1 (MQ=255)

                   gcgcCTGCACCACCACCGAGCCTTTCGCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATCCCACGTTCCGAGAACGGATTCa                                                                       <  1:2802743/93‑1 (MQ=255)

                    cgcCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAc                                                                      <  1:6274232/93‑1 (MQ=255)

                     gcgtGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAcc                                                                     <  1:4858660/90‑1 (MQ=255)

                      ccTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccg                                                                    <  2:707186/93‑1 (MQ=255)

                       cTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgc                                                                   >  1:3380308/1‑93 (MQ=255)

                        tGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgcc                                                                  <  2:6096970/93‑1 (MQ=255)

                        tGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgcc                                                                  >  2:10535/1‑93 (MQ=255)

                         gCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAccgccg                                                                 >  1:1204132/1‑93 (MQ=255)

                          cTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACcgccgc                                                                <  2:1239262/93‑1 (MQ=255)

                           tCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCgccgcc                                                               >  1:4690453/1‑93 (MQ=255)

                            ccGCCACTGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCt                                                              <  2:1287628/93‑1 (MQ=255)

                             cGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTg                                                             <  2:3638528/93‑1 (MQ=255)

                             cGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTg                                                             <  2:6125993/93‑1 (MQ=255)

                             cGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTg                                                             <  2:5946711/93‑1 (MQ=255)

                             cGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTg                                                             >  1:2362850/1‑93 (MQ=255)

                                cACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCt                                                          <  1:3481437/93‑1 (MQ=255)

                                  ccGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGc                                                        >  2:1276600/1‑93 (MQ=255)

                                   cGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGcc                                                       <  2:3881688/93‑1 (MQ=255)

                                    gAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCt                                                      <  1:5321209/93‑1 (MQ=255)

                                    gAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCt                                                      <  1:989705/93‑1 (MQ=255)

                                    gAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCt                                                      >  1:21245/1‑93 (MQ=255)

                                     aGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCtt                                                     >  2:5370452/1‑93 (MQ=255)

                                      gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTTGCTGCCtgt                                                    >  1:2646531/1‑91 (MQ=255)

                                      gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCttt                                                    <  2:5025729/93‑1 (MQ=255)

                                      gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCttt                                                    <  1:1237483/93‑1 (MQ=255)

                                      gCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCttt                                                    <  1:457547/93‑1 (MQ=255)

                                       ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTc                                                   <  1:2309583/93‑1 (MQ=255)

                                       ccTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTc                                                   <  1:84111/93‑1 (MQ=255)

                                           tCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGt                                               <  2:3490102/93‑1 (MQ=255)

                                               cAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACg                                           <  2:2201151/93‑1 (MQ=255)

                                               cAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACg                                           >  1:3104919/1‑93 (MQ=255)

                                                 gcgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGtt                                         <  1:642275/93‑1 (MQ=255)

                                                  cgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTg                                        <  1:4870640/93‑1 (MQ=255)

                                                  cgcATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTg                                        <  2:2352571/93‑1 (MQ=255)

                                                    cATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGAt                                      <  2:2365212/93‑1 (MQ=255)

                                                      tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATaa                                    >  1:2003107/1‑93 (MQ=255)

                                                      tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATaa                                    >  1:4472080/1‑93 (MQ=255)

                                                      tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATaa                                    <  1:4299860/93‑1 (MQ=255)

                                                      tCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATaa                                    <  1:2246526/93‑1 (MQ=255)

                                                       ccTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAt                                   <  2:442390/93‑1 (MQ=255)

                                                        cTGCGCGAGGAAATTTTGCCGCGCATCGTCGATTCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAtt                                  <  1:1911228/93‑1 (MQ=255)

                                                        cTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAtt                                  >  1:1806781/1‑93 (MQ=255)

                                                        cTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAtt                                  <  2:3044494/93‑1 (MQ=255)

                                                           cgcgAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAttttt                               <  2:5122275/93‑1 (MQ=255)

                                                            gcgAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTa                              >  1:2702634/1‑93 (MQ=255)

                                                             cgAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTaa                             >  2:2672370/1‑93 (MQ=255)

                                                              gAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTaaa                            <  2:1817768/93‑1 (MQ=255)

                                                              gAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTaaa                            >  2:2084367/1‑93 (MQ=255)

                                                               aGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAc                           >  1:359816/1‑93 (MQ=255)

                                                               aGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAc                           >  1:1521045/1‑93 (MQ=255)

                                                                  aaaaTTTGCCGCGCATCGTCGATTCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGa                        <  1:783372/93‑1 (MQ=255)

                                                                  aaaTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGa                        <  1:866139/93‑1 (MQ=255)

                                                                  aaaTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGa                        <  2:4529491/93‑1 (MQ=255)

                                                                    aTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACg                      <  1:4415277/93‑1 (MQ=255)

                                                                    aTTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACg                      <  2:6294336/93‑1 (MQ=255)

                                                                     ttttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                     <  1:1441560/93‑1 (MQ=255)

                                                                     ttttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACgg                     <  2:1020844/93‑1 (MQ=255)

                                                                      tttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                    <  2:4634856/93‑1 (MQ=255)

                                                                      tttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                    <  1:2529506/93‑1 (MQ=255)

                                                                      tttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGt                    <  2:5444028/93‑1 (MQ=255)

                                                                       ttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTc                   >  2:2947609/1‑93 (MQ=255)

                                                                       ttGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTc                   >  1:6066005/1‑93 (MQ=255)

                                                                         gCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTcgc                 >  2:6005371/1‑93 (MQ=255)

                                                                            gcgcATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCt              >  2:3838502/1‑93 (MQ=255)

                                                                             cgcATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTc             <  1:1021116/93‑1 (MQ=255)

                                                                               cATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCg           >  2:4088438/1‑93 (MQ=255)

                                                                                 tcgtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCt         >  1:4510477/1‑93 (MQ=255)

                                                                                   gtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGa       >  1:1072066/1‑93 (MQ=255)

                                                                                   gtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGa       >  1:2936533/1‑93 (MQ=255)

                                                                                   gtcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGa       >  1:38094/1‑93 (MQ=255)

                                                                                    tcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGaa      >  1:1637921/1‑93 (MQ=255)

                                                                                    tcgATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGaa      >  2:1329950/1‑93 (MQ=255)

                                                                                      gATGCCACGTTCCGAGATCGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAc    <  1:5616964/93‑1 (MQ=255)

                                                                                      gATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTTAACAGACGGTCGCGCTCCGCTGAAAc    >  2:3278401/1‑93 (MQ=255)

                                                                                      gATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAc    <  1:4095129/93‑1 (MQ=255)

                                                                                      gATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAc    <  2:1605363/93‑1 (MQ=255)

TCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT  >  NC_000913/3768429‑3768609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGG  >  NC_000913/3768427‑3768612

TGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCC                                                                                              >  SRR4302533.5841969/1‑94 (MQ=60)

  TCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAC                                                                                            <  SRR4302533.2557714/94‑1 (MQ=60)

  TCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCAC                                                                                            <  SRR4302533.5805501/94‑1 (MQ=60)

   GGAGCTGGCTTTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACG                                                                                           <  SRR4302533.185411/94‑1 (MQ=60)

      GCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATTCCACGTTC                                                                                        <  SRR4302533.2144428/94‑1 (MQ=60)

      GCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTC                                                                                        >  SRR4302533.5169063/1‑94 (MQ=60)

       CTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCC                                                                                       >  SRR4302533.993020/1‑94 (MQ=60)

       CTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCC                                                                                       >  SRR4302533.4590737/1‑94 (MQ=60)

        TGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCG                                                                                      >  SRR4302533.5949793/1‑94 (MQ=60)

        TGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCG                                                                                      <  SRR4302533.3499207/94‑1 (MQ=60)

        TGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCG                                                                                      >  SRR4302533.4774364/1‑94 (MQ=60)

          GCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAG                                                                                    <  SRR4302533.2745090/94‑1 (MQ=60)

          GCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAG                                                                                    <  SRR4302533.4506759/94‑1 (MQ=60)

          GCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAG                                                                                    >  SRR4302533.1949296/1‑94 (MQ=60)

           CTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGA                                                                                   >  SRR4302533.3149540/1‑94 (MQ=60)

            TCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAA                                                                                  <  SRR4302533.389868/94‑1 (MQ=60)

            TCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAA                                                                                  >  SRR4302533.2385091/1‑94 (MQ=60)

             CTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAAC                                                                                 <  SRR4302533.4160344/94‑1 (MQ=60)

             CTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAAC                                                                                 <  SRR4302533.2002804/94‑1 (MQ=60)

              TGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACG                                                                                >  SRR4302533.3854422/1‑94 (MQ=60)

               GGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGG                                                                               >  SRR4302533.2165407/1‑94 (MQ=60)

                GATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGA                                                                              <  SRR4302533.5723761/94‑1 (MQ=60)

                GATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGA                                                                              <  SRR4302533.1357315/94‑1 (MQ=60)

                  TCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATT                                                                            >  SRR4302533.5754992/1‑94 (MQ=60)

                   CTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTC                                                                           <  SRR4302533.2540308/94‑1 (MQ=60)

                    TGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCA                                                                          <  SRR4302533.2712463/94‑1 (MQ=60)

                    TGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCA                                                                          >  SRR4302533.4865906/1‑94 (MQ=60)

                     GCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCAC                                                                         <  SRR4302533.6056187/94‑1 (MQ=60)

                     GCGCCTGCACCACCACCGAGCCTTTCGCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATCCCACGTTCCGAGAACGGATTCAC                                                                         <  SRR4302533.2819992/94‑1 (MQ=60)

                      CGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACC                                                                        <  SRR4302533.4776064/94‑1 (MQ=60)

                      CGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACC                                                                        >  SRR4302533.292260/1‑94 (MQ=60)

                      CGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACC                                                                        >  SRR4302533.5658517/1‑94 (MQ=60)

                      CGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACC                                                                        <  SRR4302533.6311706/94‑1 (MQ=60)

                       GCGTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCG                                                                       <  SRR4302533.4888109/94‑1 (MQ=60)

                       GCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCG                                                                       >  SRR4302533.2002872/1‑94 (MQ=60)

                        CCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGC                                                                      >  SRR4302533.3401175/1‑94 (MQ=60)

                         CTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCC                                                                     >  SRR4302533.4130954/1‑94 (MQ=60)

                         CTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCC                                                                     <  SRR4302533.1990823/94‑1 (MQ=60)

                         CTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCC                                                                     <  SRR4302533.4574463/94‑1 (MQ=60)

                         CTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCC                                                                     >  SRR4302533.1610576/1‑94 (MQ=60)

                          TGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCG                                                                    >  SRR4302533.1211370/1‑94 (MQ=60)

                           GCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGC                                                                   >  SRR4302533.2103630/1‑94 (MQ=60)

                            CTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCC                                                                  <  SRR4302533.6081434/94‑1 (MQ=60)

                            CTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCC                                                                  >  SRR4302533.4718886/1‑94 (MQ=60)

                              CCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTG                                                                >  SRR4302533.2377469/1‑94 (MQ=60)

                               CGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGG                                                               <  SRR4302533.399766/94‑1 (MQ=60)

                               CGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGG                                                               <  SRR4302533.6041587/94‑1 (MQ=60)

                                 CCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCT                                                             <  SRR4302533.2709342/94‑1 (MQ=60)

                                  CACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTG                                                            <  SRR4302533.3502912/94‑1 (MQ=60)

                                   ACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGC                                                           >  SRR4302533.4238191/1‑94 (MQ=60)

                                     CGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCT                                                         >  SRR4302533.21386/1‑94 (MQ=60)

                                      GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTT                                                        <  SRR4302533.5353098/94‑1 (MQ=60)

                                      GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTT                                                        >  SRR4302533.1065951/1‑94 (MQ=60)

                                      GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTT                                                        <  SRR4302533.6192808/94‑1 (MQ=60)

                                      GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTT                                                        <  SRR4302533.995496/94‑1 (MQ=60)

                                      GAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTT                                                        <  SRR4302533.2299048/94‑1 (MQ=60)

                                       AGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTTGCTGCCTGT                                                       >  SRR4302533.2662792/1‑94 (MQ=60)

                                        GCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTC                                                      >  SRR4302533.1487193/1‑94 (MQ=60)

                                        GCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTC                                                      <  SRR4302533.460284/94‑1 (MQ=60)

                                        GCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTC                                                      <  SRR4302533.1244874/94‑1 (MQ=60)

                                        GCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTC                                                      <  SRR4302533.854833/94‑1 (MQ=60)

                                         CCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCA                                                     <  SRR4302533.1713242/94‑1 (MQ=60)

                                         CCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCA                                                     <  SRR4302533.2323890/94‑1 (MQ=60)

                                         CCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCA                                                     <  SRR4302533.2348954/94‑1 (MQ=60)

                                         CCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCA                                                     <  SRR4302533.84646/94‑1 (MQ=60)

                                          CTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAA                                                    >  SRR4302533.6190270/1‑94 (MQ=60)

                                          CTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAA                                                    >  SRR4302533.2168862/1‑94 (MQ=60)

                                          CTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAA                                                    >  SRR4302533.2358879/1‑94 (MQ=60)

                                           TTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAG                                                   <  SRR4302533.4476841/94‑1 (MQ=60)

                                           TTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAG                                                   <  SRR4302533.3432992/94‑1 (MQ=60)

                                            TTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGT                                                  >  SRR4302533.1447226/1‑94 (MQ=60)

                                             TCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTA                                                 <  SRR4302533.409430/94‑1 (MQ=60)

                                             TCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTA                                                 >  SRR4302533.243711/1‑94 (MQ=60)

                                               CCCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAAC                                               <  SRR4302533.196478/94‑1 (MQ=60)

                                                CCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACG                                              >  SRR4302533.3124005/1‑94 (MQ=60)

                                                 CAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGT                                             >  SRR4302533.3749801/1‑94 (MQ=60)

                                                  AGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTT                                            <  SRR4302533.4658049/94‑1 (MQ=60)

                                                  AGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTT                                            >  SRR4302533.3242663/1‑94 (MQ=60)

                                                   GCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTG                                           <  SRR4302533.646052/94‑1 (MQ=60)

                                                   GCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTG                                           <  SRR4302533.4605097/94‑1 (MQ=60)

                                                    CGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGA                                          >  SRR4302533.5743011/1‑94 (MQ=60)

                                                    CGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGA                                          <  SRR4302533.4900156/94‑1 (MQ=60)

                                                     GCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGAT                                         >  SRR4302533.5632130/1‑94 (MQ=60)

                                                     GCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGAT                                         >  SRR4302533.4591822/1‑94 (MQ=60)

                                                       ATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAA                                       >  SRR4302533.4499129/1‑94 (MQ=60)

                                                       ATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAA                                       >  SRR4302533.2015445/1‑94 (MQ=60)

                                                       ATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAA                                       <  SRR4302533.4578559/94‑1 (MQ=60)

                                                       ATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAA                                       >  SRR4302533.1138635/1‑94 (MQ=60)

                                                        TCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAT                                      <  SRR4302533.4325718/94‑1 (MQ=60)

                                                        TCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAAT                                      <  SRR4302533.2260409/94‑1 (MQ=60)

                                                         CCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATT                                     >  SRR4302533.6344515/1‑94 (MQ=60)

                                                         CCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATT                                     >  SRR4302533.1817777/1‑94 (MQ=60)

                                                          CTGCGCGAGGAAATTTTGCCGCGCATCGTCGATTCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTT                                    <  SRR4302533.1922891/94‑1 (MQ=60)

                                                             CGCGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTA                                 >  SRR4302533.2719211/1‑94 (MQ=60)

                                                               CGAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAA                               >  SRR4302533.5728042/1‑94 (MQ=60)

                                                                GAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAC                              >  SRR4302533.362006/1‑94 (MQ=60)

                                                                GAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAC                              <  SRR4302533.944121/94‑1 (MQ=60)

                                                                GAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAC                              <  SRR4302533.2016557/94‑1 (MQ=60)

                                                                GAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAC                              >  SRR4302533.1530202/1‑94 (MQ=60)

                                                                GAGGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAAC                              >  SRR4302533.4178492/1‑94 (MQ=60)

                                                                  GGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAG                            <  SRR4302533.2212689/94‑1 (MQ=60)

                                                                  GGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAG                            <  SRR4302533.1912276/94‑1 (MQ=60)

                                                                  GGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAG                            <  SRR4302533.5803902/94‑1 (MQ=60)

                                                                  GGAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAG                            >  SRR4302533.4894597/1‑94 (MQ=60)

                                                                   GAAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGA                           <  SRR4302533.5368826/94‑1 (MQ=60)

                                                                    AAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAC                          <  SRR4302533.871212/94‑1 (MQ=60)

                                                                    AAATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGACTGGATGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAC                          <  SRR4302533.6334682/94‑1 (MQ=60)

                                                                    AAAATTTGCCGCGCATCGTCGATTCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGAC                          <  SRR4302533.787979/94‑1 (MQ=60)

                                                                      ATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGG                        <  SRR4302533.5005468/94‑1 (MQ=60)

                                                                      ATTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGG                        <  SRR4302533.4441940/94‑1 (MQ=60)

                                                                       TTTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGT                       <  SRR4302533.1450260/94‑1 (MQ=60)

                                                                        TTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTC                      >  SRR4302533.6102717/1‑94 (MQ=60)

                                                                        TTTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTC                      <  SRR4302533.2545079/94‑1 (MQ=60)

                                                                         TTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                     <  SRR4302533.5331012/94‑1 (MQ=60)

                                                                         TTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                     <  SRR4302533.1926735/94‑1 (MQ=60)

                                                                         TTGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCG                     <  SRR4302533.2114489/94‑1 (MQ=60)

                                                                          TGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGC                    <  SRR4302533.1621671/94‑1 (MQ=60)

                                                                          TGCCGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGATGATAATTTTTAAACAGACGGTCGC                    >  SRR4302533.2876630/1‑94 (MQ=60)

                                                                             CGCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCT                 >  SRR4302533.4737928/1‑94 (MQ=60)

                                                                              GCGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTCGATAATTTTTAAACAGACGGTCGCGCTC                >  SRR4302533.772359/1‑94 (MQ=60)

                                                                               CGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCC               <  SRR4302533.1027116/94‑1 (MQ=60)

                                                                               CGCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCC               <  SRR4302533.3823341/94‑1 (MQ=60)

                                                                                GCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCG              <  SRR4302533.1118845/94‑1 (MQ=60)

                                                                                GCATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCG              <  SRR4302533.3533705/94‑1 (MQ=60)

                                                                                  ATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT            >  SRR4302533.2378681/1‑94 (MQ=60)

                                                                                  ATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT            >  SRR4302533.4537793/1‑94 (MQ=60)

                                                                                  ATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT            >  SRR4302533.4935548/1‑94 (MQ=60)

                                                                                  ATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT            >  SRR4302533.1221369/1‑94 (MQ=60)

                                                                                  ATCGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCT            >  SRR4302533.448360/1‑94 (MQ=60)

                                                                                    CGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGA          >  SRR4302533.1078382/1‑94 (MQ=60)

                                                                                    CGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGA          >  SRR4302533.2954485/1‑94 (MQ=60)

                                                                                    CGTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGA          >  SRR4302533.38345/1‑94 (MQ=60)

                                                                                     GTCGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAA         >  SRR4302533.1647837/1‑94 (MQ=60)

                                                                                       CGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAC       <  SRR4302533.799358/94‑1 (MQ=60)

                                                                                       CGATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAAC       >  SRR4302533.80060/1‑94 (MQ=60)

                                                                                        GATGCCACGTTCCGAGATCGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACC      <  SRR4302533.5650918/94‑1 (MQ=60)

                                                                                        GATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACC      <  SRR4302533.4120061/94‑1 (MQ=60)

                                                                                        GATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACC      >  SRR4302533.3067269/1‑94 (MQ=60)

                                                                                         ATGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCT     >  SRR4302533.1240772/1‑94 (MQ=60)

                                                                                         ATGCCACGTCCCGAGAACGAATTCACCG‑CGGCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG    <  SRR4302533.4349052/94‑1 (MQ=60)

                                                                                          TGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG    >  SRR4302533.6034131/1‑94 (MQ=60)

                                                                                          TGCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTG    <  SRR4302533.5735756/94‑1 (MQ=60)

                                                                                           GCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGG   <  SRR4302533.3721982/94‑1 (MQ=60)

                                                                                           GCCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGG   <  SRR4302533.1367343/94‑1 (MQ=60)

                                                                                            CCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGG  >  SRR4302533.2334850/1‑94 (MQ=60)

                                                                                            CCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGG  <  SRR4302533.1338196/94‑1 (MQ=60)

TGTCGAGCTGGCTCTGGATCTGCGCCTGCTCCGCCACCGAGCCTTTCACCAGCGCATCCTGCGCGAGGAAATTTTGCCGCGCATCGTCGATGTCACGTTCCGAGAACGGATTCACCGCCGCCTGGCTGCCTTTCAAGTAACGTTGATAATTTTTAAACAGACGGTCGCGCTCCGCTGAAACCTGGG  >  NC_000913/3768427‑3768612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`