Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,881,483 Δ1 bp intergenic (‑126/‑482) dnaA ← / → rpmH DnaA/50S ribosomal subunit protein L34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,881,4830G.100.0% 1261.7 / NA 273intergenic (‑126/‑482)dnaA/rpmHDnaA/50S ribosomal subunit protein L34
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (148/125);  total (148/125)

Only 100 of 279 total aligned reads displayed.

GGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAGTCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTA  >  NC_000913/3881395‑3881570
                                                                                        |                                                                                       
ggCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCACGA‑TCAAg                                                                                    >  1:3947363/1‑93 (MQ=255)
  cTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGAt                                                                                  <  1:5181722/93‑1 (MQ=255)
   tGAAACATTCATGATTGTTGACGTACGTCGAAAAGCCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATc                                                                                 <  1:1715901/93‑1 (MQ=255)
   tGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGAGCGAG‑CAAGATc                                                                                 >  2:1602136/1‑93 (MQ=255)
    gAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATcc                                                                                >  2:3590613/1‑93 (MQ=255)
     aaaCATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCt                                                                               >  1:782279/1‑93 (MQ=255)
     aaaCATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCt                                                                               >  1:3502822/1‑93 (MQ=255)
       aCATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGc                                                                             >  1:5939453/1‑93 (MQ=255)
       aCATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGc                                                                             <  2:691772/93‑1 (MQ=255)
       aCATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGc                                                                             >  2:4118706/1‑93 (MQ=255)
        cATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCa                                                                            >  1:616158/1‑93 (MQ=255)
         aTTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaa                                                                           >  2:4849837/1‑93 (MQ=255)
          ttCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaa                                                                          >  1:5718678/1‑93 (MQ=255)
           tCATGATTGTTGACGTACTTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaaa                                                                         <  2:3947943/93‑1 (MQ=255)
           tCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaaa                                                                         <  2:2327115/93‑1 (MQ=255)
           tCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaaa                                                                         <  1:1144609/93‑1 (MQ=255)
           tCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaaa                                                                         <  2:463542/93‑1 (MQ=255)
           tCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCaaaa                                                                         <  1:1982235/93‑1 (MQ=255)
            cATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGAGGTTATATGCCCGA‑TCAAGATCCTGCAAAAc                                                                        >  1:1566260/1‑93 (MQ=255)
            cATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGAGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAc                                                                        >  2:4796766/1‑93 (MQ=255)
              tGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCACAACGa                                                                      >  2:5870151/1‑93 (MQ=255)
              tGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGa                                                                      <  1:5389854/93‑1 (MQ=255)
               gATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGAt                                                                     <  2:2005035/93‑1 (MQ=255)
               gATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGAt                                                                     <  1:3925375/93‑1 (MQ=255)
                aTTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATc                                                                    <  1:4734080/93‑1 (MQ=255)
                aTTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATc                                                                    <  1:5338290/93‑1 (MQ=255)
                  tgttgACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCgg                                                                  <  1:1399283/93‑1 (MQ=255)
                   gttgACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCggg                                                                 >  2:1096732/1‑93 (MQ=255)
                   gttgACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCggg                                                                 >  1:1861/1‑93 (MQ=255)
                     tgACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAc                                                               >  1:6103891/1‑93 (MQ=255)
                     tgACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAc                                                               <  2:2622619/93‑1 (MQ=255)
                      gACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAcc                                                              >  1:4213670/1‑93 (MQ=255)
                      gACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAcc                                                              <  1:2761313/93‑1 (MQ=255)
                       acgtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACcg                                                             >  2:517101/1‑93 (MQ=255)
                        cgtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACcgc                                                            >  1:1050650/1‑93 (MQ=255)
                        cgtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACcgc                                                            >  1:462710/1‑93 (MQ=255)
                        cgtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACcgc                                                            <  2:5801915/93‑1 (MQ=255)
                         gtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAGcgcg                                                           >  2:1607496/1‑93 (MQ=255)
                         gtacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACcgcg                                                           >  1:6230134/1‑93 (MQ=255)
                          tacgtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCgg                                                          <  2:1441174/93‑1 (MQ=255)
                           acgtCGAAAAGACCCTGCTTGAGGGGGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAGAACGATCGGGACCGCggg                                                         >  2:1171019/1‑92 (MQ=255)
                             gtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATc                                                       >  1:4865711/1‑93 (MQ=255)
                             gtCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATc                                                       >  1:2209085/1‑93 (MQ=255)
                              tCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCa                                                      <  1:1383485/93‑1 (MQ=255)
                                gAAAAGCCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    <  1:4329806/93‑1 (MQ=255)
                                gAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    >  2:4928969/1‑93 (MQ=255)
                                gAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    <  2:1353775/93‑1 (MQ=255)
                                gAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    >  1:248615/1‑93 (MQ=255)
                                gAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    <  2:556774/93‑1 (MQ=255)
                                gAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATa                                                    >  2:6102546/1‑93 (MQ=255)
                                 aaaaGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAg                                                   >  1:3294258/1‑93 (MQ=255)
                                  aaaGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGc                                                  <  1:3697862/93‑1 (MQ=255)
                                   aaGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGcc                                                 >  1:3434144/1‑93 (MQ=255)
                                    aGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAGACGATCGGGACCGCGGATCATAGCCt                                                >  2:91705/1‑93 (MQ=255)
                                      gcccTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTaa                                              <  2:6210831/92‑1 (MQ=255)
                                       cccTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTaaa                                             >  1:2394061/1‑93 (MQ=255)
                                       cccTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTaaa                                             <  1:2889819/93‑1 (MQ=255)
                                       cccTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTaaa                                             >  2:4452378/1‑93 (MQ=255)
                                        ccTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAAc                                            >  2:3497392/1‑93 (MQ=255)
                                         cTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACt                                           >  1:2853146/1‑93 (MQ=255)
                                         cTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACt                                           <  2:4752799/93‑1 (MQ=255)
                                         cTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACt                                           <  1:3730996/93‑1 (MQ=255)
                                          tGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTg                                          >  2:4260333/1‑93 (MQ=255)
                                          tGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTg                                          >  2:2149386/1‑93 (MQ=255)
                                           gCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTgc                                         >  2:2278295/1‑93 (MQ=255)
                                             ttGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTgcgc                                       >  2:4806702/1‑93 (MQ=255)
                                             ttGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTgcgc                                       >  1:4152256/1‑93 (MQ=255)
                                                  ggTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAagag                                  >  1:5541504/1‑93 (MQ=255)
                                                   gTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAgaga                                 <  1:4959142/93‑1 (MQ=255)
                                                    tGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAt                                <  1:4074443/93‑1 (MQ=255)
                                                    ggACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAt                                <  1:3819964/92‑1 (MQ=255)
                                                     gACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAtc                               <  2:2690707/93‑1 (MQ=255)
                                                     gACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAtc                               >  1:2336836/1‑93 (MQ=255)
                                                      aCGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAtct                              <  2:4332214/93‑1 (MQ=255)
                                                        gCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGACAtcttc                            <  2:5241992/93‑1 (MQ=255)
                                                         cACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAtcttct                           <  2:3645638/93‑1 (MQ=255)
                                                          aCGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTg                          >  1:948997/1‑93 (MQ=255)
                                                           cGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGt                         <  1:2097849/93‑1 (MQ=255)
                                                             aaCCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGttt                       <  1:2130301/93‑1 (MQ=255)
                                                              aCCGCTGTCTGCGGTTATATGCCGGA‑TCAAGATCCTGCAAAACGATAGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTtc                      >  2:5073171/1‑93 (MQ=255)
                                                                cGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTtctc                    >  2:569596/1‑93 (MQ=255)
                                                                 gCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTca                   >  1:3071207/1‑93 (MQ=255)
                                                                  cTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTcac                  >  1:647329/1‑93 (MQ=255)
                                                                   tGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTcaca                 <  2:1831027/93‑1 (MQ=255)
                                                                   tGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTcaca                 >  1:4943102/1‑93 (MQ=255)
                                                                   tGTCTGCGGTTATATGCCC‑ATTAAAGATCCTGCAAACCGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTATCTcaca                 <  2:3194097/93‑1 (MQ=255)
                                                                    gTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAg                >  2:497205/1‑93 (MQ=255)
                                                                    gTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAg                >  2:5026250/1‑93 (MQ=255)
                                                                    gTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAg                >  1:5106235/1‑93 (MQ=255)
                                                                     tCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGa               >  2:2500096/1‑93 (MQ=255)
                                                                     tCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGa               <  1:1330853/93‑1 (MQ=255)
                                                                     tCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGa               >  2:5966572/1‑93 (MQ=255)
                                                                     tCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGa               >  1:2556664/1‑93 (MQ=255)
                                                                      cTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGAt              >  2:1922327/1‑93 (MQ=255)
                                                                      cTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGAt              >  2:6141836/1‑93 (MQ=255)
                                                                       tGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGAtt             <  1:2993557/93‑1 (MQ=255)
                                                                          ggTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTc          >  2:5457889/1‑93 (MQ=255)
                                                                           gTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTcc         >  1:1238269/1‑93 (MQ=255)
                                                                                  gCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTa  >  2:2899976/1‑93 (MQ=255)
                                                                                  gCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTa  >  1:3357560/1‑93 (MQ=255)
                                                                                        |                                                                                       
GGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAGTCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTA  >  NC_000913/3881395‑3881570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTAAGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAGTCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTATCC  >  NC_000913/3881390‑3881573
                                                                                             |                                                                                          
ACTAAGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAT                                                                                            >  SRR4302533.2124075/1‑94 (MQ=60)
ACTAAGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAT                                                                                            >  SRR4302533.5909094/1‑94 (MQ=60)
   AAGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAA                                                                                        >  SRR4302533.5153778/1‑94 (MQ=60)
    AGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGGCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAG                                                                                       <  SRR4302533.2245597/94‑1 (MQ=60)
    AGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCACGA‑TCAAG                                                                                       >  SRR4302533.3971435/1‑94 (MQ=60)
     GGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGA                                                                                      <  SRR4302533.3729915/94‑1 (MQ=60)
     GGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGA                                                                                      >  SRR4302533.2432103/1‑94 (MQ=60)
     GGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGA                                                                                      >  SRR4302533.1470970/1‑94 (MQ=60)
      GCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGAT                                                                                     <  SRR4302533.2183838/94‑1 (MQ=60)
      GCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGAT                                                                                     >  SRR4302533.6023267/1‑94 (MQ=60)
       CTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATC                                                                                    >  SRR4302533.1399050/1‑94 (MQ=60)
       CTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATC                                                                                    <  SRR4302533.4875773/94‑1 (MQ=60)
       CTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATC                                                                                    <  SRR4302533.5213010/94‑1 (MQ=60)
       CTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATC                                                                                    <  SRR4302533.2367564/94‑1 (MQ=60)
       CTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATC                                                                                    <  SRR4302533.922531/94‑1 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGCCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCC                                                                                   <  SRR4302533.1726304/94‑1 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCC                                                                                   >  SRR4302533.3723858/1‑94 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCC                                                                                   >  SRR4302533.934292/1‑94 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCC                                                                                   >  SRR4302533.2878301/1‑94 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCC                                                                                   >  SRR4302533.744835/1‑94 (MQ=60)
        TGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATAC                                                                                   >  SRR4302533.135428/1‑94 (MQ=60)
         GAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCT                                                                                  >  SRR4302533.3524419/1‑94 (MQ=60)
         GAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCT                                                                                  <  SRR4302533.3916288/94‑1 (MQ=60)
         GAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCT                                                                                  >  SRR4302533.786882/1‑94 (MQ=60)
          AAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTG                                                                                 >  SRR4302533.3518786/1‑94 (MQ=60)
          AAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTG                                                                                 >  SRR4302533.5365302/1‑94 (MQ=60)
          AAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTG                                                                                 <  SRR4302533.730616/94‑1 (MQ=60)
           AACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGC                                                                                >  SRR4302533.5975366/1‑94 (MQ=60)
           AACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGC                                                                                >  SRR4302533.2569094/1‑94 (MQ=60)
            ACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCA                                                                               <  SRR4302533.4124217/94‑1 (MQ=60)
            ACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCA                                                                               >  SRR4302533.619777/1‑94 (MQ=60)
              ATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAA                                                                             >  SRR4302533.5159958/1‑94 (MQ=60)
              ATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAA                                                                             >  SRR4302533.3321274/1‑94 (MQ=60)
              ATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAA                                                                             >  SRR4302533.3808784/1‑94 (MQ=60)
              ATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAA                                                                             >  SRR4302533.5753209/1‑94 (MQ=60)
               TTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAA                                                                            >  SRR4302533.2801757/1‑94 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           >  SRR4302533.1687565/1‑94 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           >  SRR4302533.2118537/1‑94 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           <  SRR4302533.1994368/94‑1 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           <  SRR4302533.6267836/94‑1 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           <  SRR4302533.1151441/94‑1 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           <  SRR4302533.2716268/94‑1 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           >  SRR4302533.131497/1‑94 (MQ=60)
                TCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGAGGTTATATGCCCGA‑TCAAGATCCTGCAAAAC                                                                           >  SRR4302533.1575691/1‑94 (MQ=60)
                 CATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACG                                                                          >  SRR4302533.3086458/1‑94 (MQ=60)
                   TGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGAT                                                                        <  SRR4302533.5422242/94‑1 (MQ=60)
                    GATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATC                                                                       <  SRR4302533.3949345/94‑1 (MQ=60)
                    GATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATC                                                                       <  SRR4302533.3294755/94‑1 (MQ=60)
                     ATTGTTGCCGTACGTCGAAAAGACCCTGCTTGAGGGTGACGGACGAACCGCTGTCTGCGGTTATCTGCCCGA‑TCAAGATCCTGCAAAACGATCG                                                                      <  SRR4302533.2702674/94‑1 (MQ=60)
                     ATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCG                                                                      <  SRR4302533.4762779/94‑1 (MQ=60)
                     ATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCG                                                                      >  SRR4302533.5182595/1‑94 (MQ=60)
                     ATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCG                                                                      <  SRR4302533.5370298/94‑1 (MQ=60)
                      TTGTTGACGTACGTCGAAAAGCCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGG                                                                     <  SRR4302533.3992052/94‑1 (MQ=60)
                       TGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGG                                                                    >  SRR4302533.1876/1‑94 (MQ=60)
                       TGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGG                                                                    <  SRR4302533.1407717/94‑1 (MQ=60)
                        GTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGA                                                                   >  SRR4302533.3653155/1‑94 (MQ=60)
                        GTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGA                                                                   >  SRR4302533.2797135/1‑94 (MQ=60)
                        GTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGA                                                                   <  SRR4302533.5405024/94‑1 (MQ=60)
                         TTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAC                                                                  <  SRR4302533.2887931/94‑1 (MQ=60)
                         TTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAC                                                                  >  SRR4302533.6140734/1‑94 (MQ=60)
                         TTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAC                                                                  <  SRR4302533.6036178/94‑1 (MQ=60)
                         TTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGAC                                                                  <  SRR4302533.1932534/94‑1 (MQ=60)
                          TGACGTACGTCGAAAAGTCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACC                                                                 <  SRR4302533.1076041/94‑1 (MQ=60)
                          TGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACC                                                                 >  SRR4302533.4239161/1‑94 (MQ=60)
                           GACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCG                                                                <  SRR4302533.2778239/94‑1 (MQ=60)
                            ACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGC                                                               >  SRR4302533.465468/1‑94 (MQ=60)
                            ACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGC                                                               >  SRR4302533.1056828/1‑94 (MQ=60)
                             CGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCG                                                              >  SRR4302533.6267447/1‑94 (MQ=60)
                                 CGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATC                                                          >  SRR4302533.2222710/1‑94 (MQ=60)
                                 CGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATC                                                          <  SRR4302533.35108/94‑1 (MQ=60)
                                 CGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATC                                                          <  SRR4302533.3951656/94‑1 (MQ=60)
                                 CGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATC                                                          >  SRR4302533.4879912/1‑94 (MQ=60)
                                 CGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATC                                                          >  SRR4302533.4895197/1‑94 (MQ=60)
                                   TCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCAT                                                        <  SRR4302533.1391810/94‑1 (MQ=60)
                                    CGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATA                                                       >  SRR4302533.3086808/1‑94 (MQ=60)
                                    CGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATA                                                       >  SRR4302533.250150/1‑94 (MQ=60)
                                     GAAAAGCCCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAG                                                      <  SRR4302533.4355891/94‑1 (MQ=60)
                                     GAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAG                                                      >  SRR4302533.3314590/1‑94 (MQ=60)
                                     GAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAG                                                      >  SRR4302533.220870/1‑94 (MQ=60)
                                       AAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCC                                                    <  SRR4302533.3720575/94‑1 (MQ=60)
                                       AAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCC                                                    >  SRR4302533.3455358/1‑94 (MQ=60)
                                          GACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAA                                                 >  SRR4302533.6339306/1‑94 (MQ=60)
                                           ACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAA                                                >  SRR4302533.2408838/1‑94 (MQ=60)
                                            CCCTGCTTGAGGGTGACGCACTAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAAC                                               >  SRR4302533.4750029/1‑94 (MQ=60)
                                            CCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAAC                                               <  SRR4302533.2907584/94‑1 (MQ=60)
                                             CCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACT                                              >  SRR4302533.2870698/1‑94 (MQ=60)
                                             CCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACT                                              <  SRR4302533.3364077/94‑1 (MQ=60)
                                              CTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTG                                             <  SRR4302533.3753880/94‑1 (MQ=60)
                                              CTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTG                                             <  SRR4302533.1380476/94‑1 (MQ=60)
                                                 CTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGC                                          >  SRR4302533.4177414/1‑94 (MQ=60)
                                                  TTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCA                                         <  SRR4302533.23776/94‑1 (MQ=60)
                                                  TTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCA                                         <  SRR4302533.2107850/94‑1 (MQ=60)
                                                    GAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAG                                       <  SRR4302533.122939/94‑1 (MQ=60)
                                                    GAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAG                                       >  SRR4302533.1582782/1‑94 (MQ=60)
                                                    GAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAG                                       >  SRR4302533.1734063/1‑94 (MQ=60)
                                                      GGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAG                                     >  SRR4302533.5574969/1‑94 (MQ=60)
                                                       GGTGACGCACGAGCCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGA                                    <  SRR4302533.498364/94‑1 (MQ=60)
                                                        GTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAT                                   <  SRR4302533.4288159/94‑1 (MQ=60)
                                                        GTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAT                                   <  SRR4302533.5753970/94‑1 (MQ=60)
                                                        GTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAT                                   <  SRR4302533.422236/94‑1 (MQ=60)
                                                        GTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAT                                   <  SRR4302533.4989186/94‑1 (MQ=60)
                                                        GTCACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGAT                                   <  SRR4302533.1466544/94‑1 (MQ=60)
                                                         TGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATC                                  >  SRR4302533.2351299/1‑94 (MQ=60)
                                                         TGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATC                                  <  SRR4302533.4099247/94‑1 (MQ=60)
                                                         GGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATC                                  <  SRR4302533.6198847/94‑1 (MQ=60)
                                                         GGACGCACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATC                                  <  SRR4302533.3843323/94‑1 (MQ=60)
                                                              CACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTG                             >  SRR4302533.5629100/1‑94 (MQ=60)
                                                              CACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTG                             <  SRR4302533.1892226/94‑1 (MQ=60)
                                                              CACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTG                             <  SRR4302533.2114361/94‑1 (MQ=60)
                                                              CACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTG                             >  SRR4302533.954570/1‑94 (MQ=60)
                                                               ACGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGT                            <  SRR4302533.4963922/94‑1 (MQ=60)
                                                                CGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTT                           <  SRR4302533.1255221/94‑1 (MQ=60)
                                                                CGAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTT                           <  SRR4302533.2110764/94‑1 (MQ=60)
                                                                 GAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTT                          <  SRR4302533.3967284/94‑1 (MQ=60)
                                                                 GAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTT                          >  SRR4302533.2529109/1‑94 (MQ=60)
                                                                 GAACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTT                          <  SRR4302533.22576/94‑1 (MQ=60)
                                                                  AACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTC                         >  SRR4302533.4608707/1‑94 (MQ=60)
                                                                  AACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTC                         <  SRR4302533.2143436/94‑1 (MQ=60)
                                                                   ACCGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCT                        >  SRR4302533.4925663/1‑94 (MQ=60)
                                                                     CGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCA                      >  SRR4302533.6177074/1‑94 (MQ=60)
                                                                     CGCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCA                      >  SRR4302533.3090061/1‑94 (MQ=60)
                                                                      GCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCAC                     >  SRR4302533.651142/1‑94 (MQ=60)
                                                                      GCTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCAC                     >  SRR4302533.3992358/1‑94 (MQ=60)
                                                                       CTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACA                    >  SRR4302533.3807050/1‑94 (MQ=60)
                                                                       CTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACA                    >  SRR4302533.4973026/1‑94 (MQ=60)
                                                                       CTGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGAAAGAGATCTTCTGTTTCTCACA                    >  SRR4302533.3161158/1‑94 (MQ=60)
                                                                        TGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAG                   >  SRR4302533.5137081/1‑94 (MQ=60)
                                                                        TGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAG                   >  SRR4302533.422915/1‑94 (MQ=60)
                                                                        TGTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAG                   <  SRR4302533.567141/94‑1 (MQ=60)
                                                                         GTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGA                  >  SRR4302533.2572387/1‑94 (MQ=60)
                                                                         GTCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGA                  <  SRR4302533.554868/94‑1 (MQ=60)
                                                                          TCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGAT                 <  SRR4302533.1338837/94‑1 (MQ=60)
                                                                          TCTGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGAT                 <  SRR4302533.5128902/94‑1 (MQ=60)
                                                                            TGCGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTT               <  SRR4302533.3011860/94‑1 (MQ=60)
                                                                              CGGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTC             <  SRR4302533.180619/94‑1 (MQ=60)
                                                                               GGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCC            <  SRR4302533.3662516/94‑1 (MQ=60)
                                                                               GGTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCC            >  SRR4302533.1245668/1‑94 (MQ=60)
                                                                                GTTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCC           <  SRR4302533.626225/94‑1 (MQ=60)
                                                                                 TTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCG          >  SRR4302533.5856105/1‑94 (MQ=60)
                                                                                 TTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCG          >  SRR4302533.5271304/1‑94 (MQ=60)
                                                                                 TTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCG          >  SRR4302533.2308475/1‑94 (MQ=60)
                                                                                 TTATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCG          >  SRR4302533.5069410/1‑94 (MQ=60)
                                                                                   ATATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGAT        <  SRR4302533.5503663/94‑1 (MQ=60)
                                                                                    TATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATT       >  SRR4302533.4119495/1‑94 (MQ=60)
                                                                                    TATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATT       <  SRR4302533.4466651/94‑1 (MQ=60)
                                                                                    TATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATT       >  SRR4302533.2997574/1‑94 (MQ=60)
                                                                                     ATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTT      >  SRR4302533.3445175/1‑94 (MQ=60)
                                                                                     ATGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTT      >  SRR4302533.4754229/1‑94 (MQ=60)
                                                                                      TGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTA     >  SRR4302533.3378308/1‑94 (MQ=60)
                                                                                      TGCCCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTA     <  SRR4302533.1204892/94‑1 (MQ=60)
                                                                                         CCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTATCC  >  SRR4302533.2511304/1‑94 (MQ=60)
                                                                                         CCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTATCC  >  SRR4302533.1313650/1‑94 (MQ=60)
                                                                                         CCGA‑TCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTATCC  >  SRR4302533.4939773/1‑94 (MQ=60)
                                                                                             |                                                                                          
ACTAAGGCTGAAACATTCATGATTGTTGACGTACGTCGAAAAGACCCTGCTTGAGGGTGACGCACGAACCGCTGTCTGCGGTTATATGCCCGAGTCAAGATCCTGCAAAACGATCGGGACCGCGGATCATAGCCTAAACTGCGCAAGAGATCTTCTGTTTCTCACAGATTTTCCCGATTTATCC  >  NC_000913/3881390‑3881573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑