Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,915,043 G→C A284G (GCC→GGC)  atpG ← membrane‑bound ATP synthase, F1 sector, gamma‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,915,0430GC99.6% 882.9 / ‑4.3 281A284G (GCC→GGC) atpGmembrane‑bound ATP synthase, F1 sector, gamma‑subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (153/127);  total (154/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 303 total aligned reads displayed.

TTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCG  >  NC_000913/3914953‑3915132
                                                                                          |                                                                                          
ttCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGccc                                                                                          >  2:1811121/1‑93 (MQ=255)
   gACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGcccccc                                                                                       >  1:3183243/1‑93 (MQ=255)
    aCGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCg                                                                                      <  2:459560/93‑1 (MQ=255)
     cGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCga                                                                                     <  1:790763/93‑1 (MQ=255)
     cGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCga                                                                                     >  1:4185212/1‑93 (MQ=255)
       tCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCgaga                                                                                   >  2:153946/1‑93 (MQ=255)
       tCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGAGCCCCCCgcgc                                                                                   >  2:1620115/1‑90 (MQ=255)
        cAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGAc                                                                                  <  1:5239901/93‑1 (MQ=255)
        cAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGAc                                                                                  >  1:5035148/1‑93 (MQ=255)
         aaCTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACg                                                                                 >  2:227923/1‑93 (MQ=255)
          aCTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGa                                                                                >  1:5742350/1‑93 (MQ=255)
          aCTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGa                                                                                >  1:2954171/1‑93 (MQ=255)
           cTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGAt                                                                               >  2:3655085/1‑93 (MQ=255)
           cTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGAt                                                                               <  1:4320260/93‑1 (MQ=255)
               ggCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCg                                                                           >  2:2326230/1‑93 (MQ=255)
                gcgcCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCgg                                                                          <  2:3192230/93‑1 (MQ=255)
                 cgcCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGt                                                                         <  1:2321778/93‑1 (MQ=255)
                 cgcCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGt                                                                         <  2:609144/93‑1 (MQ=255)
                   ccGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGa                                                                       >  1:2694535/1‑93 (MQ=255)
                     gATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGt                                                                     >  1:3647918/1‑93 (MQ=255)
                     gATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGt                                                                     >  2:6201001/1‑93 (MQ=255)
                     gATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGt                                                                     >  1:3763407/1‑93 (MQ=255)
                       ttACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTc                                                                   >  1:2376851/1‑93 (MQ=255)
                        tACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTcc                                                                  >  1:136918/1‑93 (MQ=255)
                        tACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTcc                                                                  >  2:5982142/1‑93 (MQ=255)
                           cTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGa                                                               <  1:3355023/93‑1 (MQ=255)
                            tGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGCTCCTGAg                                                              >  1:2563435/1‑93 (MQ=255)
                             ggACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGt                                                             >  1:4108106/1‑93 (MQ=255)
                               aCAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTaa                                                           >  2:5615378/1‑93 (MQ=255)
                               aCAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTaa                                                           <  1:1684472/93‑1 (MQ=255)
                                cAATCTTTCCAGTAGCCATCTTAAATCCTCTGCGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAt                                                          <  1:4224176/93‑1 (MQ=255)
                                    cTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTg                                                      >  1:5410034/1‑93 (MQ=255)
                                    cTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTg                                                      <  1:681744/93‑1 (MQ=255)
                                        ccAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCt                                                  <  2:5483979/93‑1 (MQ=255)
                                          aGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGa                                                <  1:3964834/93‑1 (MQ=255)
                                          aGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGa                                                >  1:4880569/1‑93 (MQ=255)
                                            tAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACg                                              <  2:1427815/93‑1 (MQ=255)
                                            tAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACg                                              >  2:5205435/1‑93 (MQ=255)
                                                cATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑Ct                                         >  2:1713477/1‑93 (MQ=255)
                                                 aTCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑Ctt                                        >  1:5871469/1‑93 (MQ=255)
                                                 aTCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑Ctt                                        >  2:5151236/1‑93 (MQ=255)
                                                 aTCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑Ctt                                        >  2:5819027/1‑93 (MQ=255)
                                                  tCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑Cttt                                       >  2:4743560/1‑93 (MQ=255)
                                                    ttAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgt                                     <  2:511866/93‑1 (MQ=255)
                                                    ttAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgt                                     >  2:4039584/1‑93 (MQ=255)
                                                    ttAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgt                                     >  2:5709712/1‑93 (MQ=255)
                                                     tAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgtt                                    <  1:122278/93‑1 (MQ=255)
                                                     tAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgtt                                    >  1:1478082/1‑93 (MQ=255)
                                                     tAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgtt                                    <  2:3979023/93‑1 (MQ=255)
                                                     tAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgtt                                    >  2:719855/1‑93 (MQ=255)
                                                      aaaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgttg                                   <  1:5495901/93‑1 (MQ=255)
                                                      aaaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgttg                                   <  2:5642977/93‑1 (MQ=255)
                                                      aaaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTttgttg                                   <  1:4836240/93‑1 (MQ=255)
                                                      aaaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTCAGTAATGCTGGCCTGACGAG‑CTttgttg                                   >  1:3035887/1‑93 (MQ=255)
                                                       aaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTtgttgt                                  <  2:2436190/93‑1 (MQ=255)
                                                       aaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTtgttgt                                  >  2:1195614/1‑93 (MQ=255)
                                                       aaTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTtgttgt                                  <  2:3385261/93‑1 (MQ=255)
                                                        aTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGta                                 <  2:85231/93‑1 (MQ=255)
                                                        aTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGta                                 <  2:4155566/93‑1 (MQ=255)
                                                        aTCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGta                                 <  2:2174206/93‑1 (MQ=255)
                                                         tCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGtat                                >  1:1055860/1‑93 (MQ=255)
                                                         tCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGtat                                >  1:3731428/1‑93 (MQ=255)
                                                          ccTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGtata                               >  2:5336638/1‑93 (MQ=255)
                                                           ctctACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATAc                              >  2:2046618/1‑93 (MQ=255)
                                                            tctACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATTTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATAcc                             <  1:872895/93‑1 (MQ=255)
                                                            tctACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATAcc                             >  1:2088183/1‑93 (MQ=255)
                                                             ctACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCa                            >  2:6055442/1‑93 (MQ=255)
                                                              tACGAAATAACCTGTTTAAACCGCAGCGCCCCCCGAGACGATCTCGGTGAGATCCTGAGTAATGCTGGCCTGACGAA‑CTTTGTTGTATAccca                           >  2:4815376/1‑91 (MQ=255)
                                                               aCGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAAc                          >  2:1915119/1‑93 (MQ=255)
                                                               aCGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAAc                          <  1:5358129/93‑1 (MQ=255)
                                                                cGAAATACCCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACt                         <  2:2736678/93‑1 (MQ=255)
                                                                cGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACt                         >  2:3000842/1‑93 (MQ=255)
                                                                cGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACt                         <  2:4649885/93‑1 (MQ=255)
                                                                  aaaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGc                       <  2:5056007/93‑1 (MQ=255)
                                                                  aaaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGc                       >  2:5863548/1‑93 (MQ=255)
                                                                  aaaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGc                       <  1:769502/93‑1 (MQ=255)
                                                                  aaaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGc                       >  1:998585/1‑93 (MQ=255)
                                                                   aaTATCCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCa                      <  2:1318751/93‑1 (MQ=255)
                                                                   aaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCa                      >  2:3462862/1‑93 (MQ=255)
                                                                   aaTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCa                      >  1:4814260/1‑93 (MQ=255)
                                                                   aaTAACCTGTTTAAACCGCGGC‑GCCCCCGGGGCGGTCTCGGGGGGGTTCTTAGGAATGGTGGGCTTAAGAGCCTTTGTT‑TATACCCAATGCAg                     >  2:4995756/1‑93 (MQ=255)
                                                                    aTAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAg                     >  1:2651657/1‑93 (MQ=255)
                                                                     tAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGc                    <  1:3884374/93‑1 (MQ=255)
                                                                        ccTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGctct                 >  1:1936906/1‑93 (MQ=255)
                                                                        ccTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGctct                 >  1:6165568/1‑93 (MQ=255)
                                                                          tGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCttt               >  2:4284889/1‑93 (MQ=255)
                                                                          tGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCttt               >  1:6077950/1‑93 (MQ=255)
                                                                          tGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCttt               <  2:3270406/93‑1 (MQ=255)
                                                                           gTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTa              >  2:2952294/1‑93 (MQ=255)
                                                                              tAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATc           >  2:4939580/1‑93 (MQ=255)
                                                                               aaaCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCa          >  1:5620916/1‑93 (MQ=255)
                                                                                aaCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAg         <  1:3597749/93‑1 (MQ=255)
                                                                                aaCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAg         >  1:2820951/1‑93 (MQ=255)
                                                                                aaCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAg         <  2:1296855/93‑1 (MQ=255)
                                                                                aaCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAg         <  1:1861302/93‑1 (MQ=255)
                                                                                 aCCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAgg        >  2:2653079/1‑93 (MQ=255)
                                                                                  ccGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGc       >  1:553173/1‑93 (MQ=255)
                                                                                   cgcgGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCt      >  1:4458591/1‑93 (MQ=255)
                                                                                     cggcgcCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgc    <  1:2269050/93‑1 (MQ=255)
                                                                                      ggcgccCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTgcc   >  2:830594/1‑93 (MQ=255)
                                                                                          |                                                                                          
TTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG‑CTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCG  >  NC_000913/3914953‑3915132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAT  >  NC_000913/3914950‑3915136
                                                                                             |                                                                                             
GAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGC                                                                                               <  SRR4302533.1078384/94‑1 (MQ=60)
 AATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCC                                                                                              <  SRR4302533.5353272/94‑1 (MQ=60)
    TCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTGCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCC                                                                                           <  SRR4302533.6192969/94‑1 (MQ=60)
     CGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCC                                                                                          <  SRR4302533.3486012/94‑1 (MQ=60)
     CGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCC                                                                                          >  SRR4302533.3202825/1‑94 (MQ=60)
     CGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCC                                                                                          >  SRR4302533.1481460/1‑94 (MQ=60)
     CGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCC                                                                                          >  SRR4302533.2754980/1‑94 (MQ=60)
       ACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGA                                                                                        >  SRR4302533.4210552/1‑94 (MQ=60)
        CGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAG                                                                                       <  SRR4302533.2391169/94‑1 (MQ=60)
        CGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAG                                                                                       <  SRR4302533.795418/94‑1 (MQ=60)
        CGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAG                                                                                       >  SRR4302533.3310988/1‑94 (MQ=60)
          TCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGAC                                                                                     >  SRR4302533.5065646/1‑94 (MQ=60)
           CAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACG                                                                                    <  SRR4302533.2677042/94‑1 (MQ=60)
           CAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACG                                                                                    <  SRR4302533.5271404/94‑1 (MQ=60)
            AACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGA                                                                                   >  SRR4302533.5777000/1‑94 (MQ=60)
            AACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGA                                                                                   <  SRR4302533.490712/94‑1 (MQ=60)
            AACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGA                                                                                   >  SRR4302533.2972238/1‑94 (MQ=60)
              CTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATC                                                                                 <  SRR4302533.4346280/94‑1 (MQ=60)
              CTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATC                                                                                 >  SRR4302533.25390/1‑94 (MQ=60)
               TACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCT                                                                                <  SRR4302533.3663501/94‑1 (MQ=60)
               TACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCT                                                                                <  SRR4302533.2068523/94‑1 (MQ=60)
                 CGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCG                                                                              <  SRR4302533.5405882/94‑1 (MQ=60)
                   GCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGT                                                                            >  SRR4302533.2039681/1‑94 (MQ=60)
                    CGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTG                                                                           <  SRR4302533.2336148/94‑1 (MQ=60)
                    CGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTG                                                                           <  SRR4302533.1083199/94‑1 (MQ=60)
                    CGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAAGCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTG                                                                           <  SRR4302533.2697979/94‑1 (MQ=60)
                     GCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGA                                                                          >  SRR4302533.4103764/1‑94 (MQ=60)
                     GCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGA                                                                          >  SRR4302533.2711047/1‑94 (MQ=60)
                       CGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGT                                                                        >  SRR4302533.3786477/1‑94 (MQ=60)
                       CGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGT                                                                        >  SRR4302533.558135/1‑94 (MQ=60)
                       CGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGT                                                                        >  SRR4302533.3670346/1‑94 (MQ=60)
                       CGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGT                                                                        >  SRR4302533.1094791/1‑94 (MQ=60)
                        GATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTT                                                                       >  SRR4302533.4061109/1‑94 (MQ=60)
                         ATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTC                                                                      >  SRR4302533.2391558/1‑94 (MQ=60)
                          TTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCC                                                                     >  SRR4302533.6061923/1‑94 (MQ=60)
                          TTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCC                                                                     >  SRR4302533.137784/1‑94 (MQ=60)
                          TTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCC                                                                     <  SRR4302533.4431886/94‑1 (MQ=60)
                           TACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCT                                                                    <  SRR4302533.5377320/94‑1 (MQ=60)
                            ACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTG                                                                   >  SRR4302533.1417984/1‑94 (MQ=60)
                             CCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGA                                                                  >  SRR4302533.4963377/1‑94 (MQ=60)
                              CTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAG                                                                 <  SRR4302533.3375754/94‑1 (MQ=60)
                              CTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAG                                                                 <  SRR4302533.5133414/94‑1 (MQ=60)
                              CTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGCTCCTGAG                                                                 >  SRR4302533.2579214/1‑94 (MQ=60)
                               TGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGT                                                                <  SRR4302533.3929981/94‑1 (MQ=60)
                               TGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGT                                                                >  SRR4302533.4133083/1‑94 (MQ=60)
                               TGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGT                                                                <  SRR4302533.3168586/94‑1 (MQ=60)
                                GGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTA                                                               >  SRR4302533.5591301/1‑94 (MQ=60)
                                GGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTA                                                               <  SRR4302533.1715385/94‑1 (MQ=60)
                                 GACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAA                                                              <  SRR4302533.3139778/94‑1 (MQ=60)
                                  ACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAT                                                             <  SRR4302533.2454980/94‑1 (MQ=60)
                                  ACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAT                                                             >  SRR4302533.2857031/1‑94 (MQ=60)
                                  ACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAT                                                             <  SRR4302533.2124845/94‑1 (MQ=60)
                                  ACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAT                                                             <  SRR4302533.1694676/94‑1 (MQ=60)
                                  ACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAAT                                                             <  SRR4302533.4241271/94‑1 (MQ=60)
                                   CAATCTTTCCAGTAGCCATCTTAAATCCTCTGCGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATG                                                            <  SRR4302533.4249713/94‑1 (MQ=60)
                                   CAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATG                                                            >  SRR4302533.3318184/1‑94 (MQ=60)
                                    AATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGC                                                           >  SRR4302533.5465766/1‑94 (MQ=60)
                                    AATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGC                                                           <  SRR4302533.3757025/94‑1 (MQ=60)
                                    AATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGC                                                           >  SRR4302533.948735/1‑94 (MQ=60)
                                    AATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGC                                                           >  SRR4302533.5761565/1‑94 (MQ=60)
                                     ATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCT                                                          <  SRR4302533.4354829/94‑1 (MQ=60)
                                     ATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCT                                                          >  SRR4302533.5185392/1‑94 (MQ=60)
                                      TCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTG                                                         >  SRR4302533.5442559/1‑94 (MQ=60)
                                       CTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGG                                                        <  SRR4302533.685775/94‑1 (MQ=60)
                                        TTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGC                                                       <  SRR4302533.1639365/94‑1 (MQ=60)
                                         TTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCC                                                      >  SRR4302533.1241946/1‑94 (MQ=60)
                                            CAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGA                                                   >  SRR4302533.4910135/1‑94 (MQ=60)
                                             AGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGAC                                                  >  SRR4302533.782311/1‑94 (MQ=60)
                                             AGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGAC                                                  <  SRR4302533.4885953/94‑1 (MQ=60)
                                             AGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGAC                                                  <  SRR4302533.3988987/94‑1 (MQ=60)
                                             AGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGAGCACCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGAC                                                  >  SRR4302533.5421296/1‑94 (MQ=60)
                                               TAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGA                                                >  SRR4302533.4845724/1‑94 (MQ=60)
                                               TAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGA                                                >  SRR4302533.4187636/1‑94 (MQ=60)
                                                AGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAG                                               >  SRR4302533.2298699/1‑94 (MQ=60)
                                                 GCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGC                                              >  SRR4302533.4976935/1‑94 (MQ=60)
                                                   CATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTT                                            >  SRR4302533.5906958/1‑94 (MQ=60)
                                                   CATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTT                                            >  SRR4302533.5922046/1‑94 (MQ=60)
                                                   CATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTT                                            <  SRR4302533.4249954/94‑1 (MQ=60)
                                                     TCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTG                                          <  SRR4302533.45727/94‑1 (MQ=60)
                                                      CTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGT                                         <  SRR4302533.2129856/94‑1 (MQ=60)
                                                      CTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGT                                         <  SRR4302533.2661338/94‑1 (MQ=60)
                                                       TTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTT                                        >  SRR4302533.1487011/1‑94 (MQ=60)
                                                        TAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTG                                       <  SRR4302533.123048/94‑1 (MQ=60)
                                                        TAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTG                                       <  SRR4302533.720661/94‑1 (MQ=60)
                                                        TAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTG                                       <  SRR4302533.5330467/94‑1 (MQ=60)
                                                        TAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTCAGTAATGCTGGCCTGACGAGCTTTGTTG                                       >  SRR4302533.3054463/1‑94 (MQ=60)
                                                         AAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGT                                      <  SRR4302533.4865553/94‑1 (MQ=60)
                                                         AAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGT                                      <  SRR4302533.5529049/94‑1 (MQ=60)
                                                          AATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTA                                     <  SRR4302533.2319800/94‑1 (MQ=60)
                                                          AATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTA                                     >  SRR4302533.3829953/1‑94 (MQ=60)
                                                           ATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTAT                                    <  SRR4302533.5823301/94‑1 (MQ=60)
                                                           ATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTAT                                    >  SRR4302533.663668/1‑94 (MQ=60)
                                                           ATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTAT                                    >  SRR4302533.1062068/1‑94 (MQ=60)
                                                           ATCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTAT                                    >  SRR4302533.3754316/1‑94 (MQ=60)
                                                            TCCTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATA                                   <  SRR4302533.4213620/94‑1 (MQ=60)
                                                            TCCTCTACGAAATAACCGGTTTAAACCGCCGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATA                                   <  SRR4302533.237358/94‑1 (MQ=60)
                                                              CTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACT                                 >  SRR4302533.5444422/1‑94 (MQ=60)
                                                              CTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACC                                 >  SRR4302533.2101042/1‑94 (MQ=60)
                                                              CTCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACC                                 >  SRR4302533.5792858/1‑94 (MQ=60)
                                                               TCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATTTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCA                                <  SRR4302533.878020/94‑1 (MQ=60)
                                                               TCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCA                                <  SRR4302533.2872915/94‑1 (MQ=60)
                                                               TCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCA                                >  SRR4302533.5548793/1‑94 (MQ=60)
                                                               TCTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCA                                >  SRR4302533.2583338/1‑94 (MQ=60)
                                                                CTACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAA                               >  SRR4302533.802509/1‑94 (MQ=60)
                                                                  ACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACT                             <  SRR4302533.4862590/94‑1 (MQ=60)
                                                                  ACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACT                             <  SRR4302533.5390292/94‑1 (MQ=60)
                                                                  ACGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACT                             <  SRR4302533.3918300/94‑1 (MQ=60)
                                                                   CGAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCCACTG                            >  SRR4302533.190684/1‑94 (MQ=60)
                                                                    GAAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGC                           >  SRR4302533.1004414/1‑94 (MQ=60)
                                                                     AAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCA                          <  SRR4302533.774033/94‑1 (MQ=60)
                                                                     AAATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCA                          >  SRR4302533.4843440/1‑94 (MQ=60)
                                                                      AATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAG                         >  SRR4302533.3705230/1‑94 (MQ=60)
                                                                      AATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAG                         >  SRR4302533.2667947/1‑94 (MQ=60)
                                                                      AATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAG                         >  SRR4302533.783690/1‑94 (MQ=60)
                                                                       ATAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATATCGGTGAGTACCTGAGTAATGCTGGTCTGACGAGCTTTGTTGTATACCAACCGCAGC                        >  SRR4302533.2006138/1‑94 (MQ=60)
                                                                        TAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCT                       <  SRR4302533.271475/94‑1 (MQ=60)
                                                                        TAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCT                       <  SRR4302533.3908074/94‑1 (MQ=60)
                                                                        TAACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCT                       >  SRR4302533.3815859/1‑94 (MQ=60)
                                                                         AACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTC                      >  SRR4302533.4149420/1‑94 (MQ=60)
                                                                         AACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCCGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTC                      >  SRR4302533.3670337/1‑94 (MQ=60)
                                                                          ACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCT                     >  SRR4302533.6202629/1‑94 (MQ=60)
                                                                          ACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCT                     >  SRR4302533.1948718/1‑94 (MQ=60)
                                                                          ACCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCT                     <  SRR4302533.3769963/94‑1 (MQ=60)
                                                                           CCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTT                    >  SRR4302533.1881104/1‑94 (MQ=60)
                                                                           CCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTT                    <  SRR4302533.5237591/94‑1 (MQ=60)
                                                                           CCTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTT                    >  SRR4302533.841158/1‑94 (MQ=60)
                                                                            CTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTT                   >  SRR4302533.1342402/1‑94 (MQ=60)
                                                                            CTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTT                   >  SRR4302533.6114710/1‑94 (MQ=60)
                                                                            CTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTT                   <  SRR4302533.1697393/94‑1 (MQ=60)
                                                                            CTGTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTT                   <  SRR4302533.907787/94‑1 (MQ=60)
                                                                              GTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAA                 >  SRR4302533.5690831/1‑94 (MQ=60)
                                                                              GTTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAA                 >  SRR4302533.353966/1‑94 (MQ=60)
                                                                               TTTAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAAT                <  SRR4302533.6231455/94‑1 (MQ=60)
                                                                                 TAAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCA              >  SRR4302533.5654890/1‑94 (MQ=60)
                                                                                  AAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCCACTGCAGCTCTTTAATCAG             >  SRR4302533.754099/1‑94 (MQ=60)
                                                                                  AAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAG             <  SRR4302533.3939869/94‑1 (MQ=60)
                                                                                  AAACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAG             >  SRR4302533.2838309/1‑94 (MQ=60)
                                                                                  AAACCGCGGCGCCCCCCGAGACGATCTCGGGGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAG             <  SRR4302533.1634094/94‑1 (MQ=60)
                                                                                  AAACCGCGGCGCCCCCCGAGACGATCTAGGTGAGTTCCTGAGTAATGGTGGCCTGACGAGGTTTGTTGTATACCAACTGCAGCTCTTTAATTAG             >  SRR4302533.2838311/1‑94 (MQ=60)
                                                                                   AACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGG            <  SRR4302533.1872650/94‑1 (MQ=60)
                                                                                   AACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGG            <  SRR4302533.3041505/94‑1 (MQ=60)
                                                                                   AACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGG            >  SRR4302533.6201859/1‑94 (MQ=60)
                                                                                   AACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGG            <  SRR4302533.3619886/94‑1 (MQ=60)
                                                                                    ACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGC           >  SRR4302533.556453/1‑94 (MQ=60)
                                                                                    ACCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGC           >  SRR4302533.5558155/1‑94 (MQ=60)
                                                                                     CCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCT          >  SRR4302533.4485558/1‑94 (MQ=60)
                                                                                     CCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCT          <  SRR4302533.4786142/94‑1 (MQ=60)
                                                                                     CCGCGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCT          <  SRR4302533.300462/94‑1 (MQ=60)
                                                                                        CGGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCC       <  SRR4302533.2283089/94‑1 (MQ=60)
                                                                                         GGCGCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCG      >  SRR4302533.4345087/1‑94 (MQ=60)
                                                                                            GCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCA   >  SRR4302533.4278961/1‑94 (MQ=60)
                                                                                            GCCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCA   >  SRR4302533.3355727/1‑94 (MQ=60)
                                                                                             CCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAT  >  SRR4302533.6269033/1‑94 (MQ=60)
                                                                                             |                                                                                             
GAATTCGACGTCAACTACGGCGCCGATTACCTGGACAATCTTTCCAGTAGCCATCTTAAATCCTCTACGAAATAACCTGTTTAAACCGCGGCGGCCCCCGAGACGATCTCGGTGAGTTCCTGAGTAATGCTGGCCTGACGAGCTTTGTTGTATACCAACTGCAGCTCTTTAATCAGGCTGCCGCCAT  >  NC_000913/3914950‑3915136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑