Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,205	A→G	F345S (TTC→TCC)	yieO ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,205	0	A	G	99.7%	930.8 / NA	284	F345S (TTC→TCC)	yieO	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (140/143); total (140/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAT  >  NC_000913/3937115‑3937294

aTCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAc                                                                                         <  2:928060/93‑1 (MQ=255)

aTCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAc                                                                                         <  1:1556608/93‑1 (MQ=255)

aTCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAc                                                                                         >  1:2174146/1‑93 (MQ=255)

 tCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACt                                                                                        <  2:2256033/93‑1 (MQ=255)

 tCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACt                                                                                        >  2:2190429/1‑93 (MQ=255)

 tCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACt                                                                                        >  1:4525288/1‑93 (MQ=255)

   gcgGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCCTTGCTGGTGATTGCAAAGAGGACTGa                                                                                      >  2:4752847/1‑93 (MQ=255)

   gcgGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGa                                                                                      <  1:101015/93‑1 (MQ=255)

   gcgGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGa                                                                                      >  1:5812917/1‑93 (MQ=255)

   gccctAAACAGCGACGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGa                                                                                      <  1:1156155/89‑1 (MQ=255)

     ggTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGc                                                                                    <  1:3094029/93‑1 (MQ=255)

      gTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCg                                                                                   <  2:1380270/93‑1 (MQ=255)

      gTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCg                                                                                   >  1:2285145/1‑93 (MQ=255)

       tAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGa                                                                                  >  2:2900159/1‑93 (MQ=255)

       tAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGa                                                                                  <  1:4827754/93‑1 (MQ=255)

        aaaCTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGAt                                                                                 <  1:945566/93‑1 (MQ=255)

          aCTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCa                                                                               >  1:519690/1‑93 (MQ=255)

               gTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGc                                                                          <  2:691967/93‑1 (MQ=255)

               ctCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGc                                                                          <  2:3812621/92‑1 (MQ=255)

                 cGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGccc                                                                        <  1:602715/93‑1 (MQ=255)

                  gACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCa                                                                       >  1:794001/1‑93 (MQ=255)

                  gACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCa                                                                       <  1:590896/93‑1 (MQ=255)

                   aCATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCaa                                                                      >  1:2137452/1‑93 (MQ=255)

                      tAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCaataa                                                                   >  2:2679648/1‑93 (MQ=255)

                        gCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATc                                                                 <  2:5657208/93‑1 (MQ=255)

                        gCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATc                                                                 >  2:194163/1‑93 (MQ=255)

                          cATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCAc                                                               <  2:2401738/93‑1 (MQ=255)

                           aTCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCAcc                                                              <  1:5014778/93‑1 (MQ=255)

                            tCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCg                                                             <  1:5383757/93‑1 (MQ=255)

                            tCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCg                                                             <  2:4307329/93‑1 (MQ=255)

                             cccTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGt                                                            >  1:3884591/1‑93 (MQ=255)

                             cccTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGt                                                            <  1:4868612/93‑1 (MQ=255)

                                   tataAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGTGCGATCATTAGCCCAATAATCACCGTGATccc                                                      >  1:253096/1‑93 (MQ=255)

                                   tataAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATccc                                                      >  2:6092000/1‑93 (MQ=255)

                                   tataAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATccc                                                      >  1:2071959/1‑93 (MQ=255)

                                   tataAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATccc                                                      >  1:2504689/1‑93 (MQ=255)

                                     taAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCa                                                    >  1:3498481/1‑93 (MQ=255)

                                     taAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCa                                                    <  2:1627792/93‑1 (MQ=255)

                                     taAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCa                                                    >  2:3381621/1‑93 (MQ=255)

                                       aacaacGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACt                                                  >  1:2376069/1‑93 (MQ=255)

                                       aacaacGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACt                                                  >  2:4833265/1‑93 (MQ=255)

                                          aacGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAAc                                               >  1:5763125/1‑93 (MQ=255)

                                          aacGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAAGAATCACCGTGATCCCCACTAAc                                               <  2:454508/93‑1 (MQ=255)

                                           acGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACg                                              >  2:1200502/1‑93 (MQ=255)

                                             ggCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCTCCGTGATCCCCACTAACGTa                                            <  1:3424470/93‑1 (MQ=255)

                                             ggCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTa                                            <  2:3231835/93‑1 (MQ=255)

                                               cAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTagg                                          <  2:3295375/93‑3 (MQ=255)

                                                aaGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATgg                                         <  1:613018/93‑1 (MQ=255)

                                                 aGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGc                                        <  2:4596518/93‑1 (MQ=255)

                                                   aTCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGa                                      <  2:4159964/93‑1 (MQ=255)

                                                    tCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAt                                     <  1:3343948/93‑1 (MQ=255)

                                                    tCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAt                                     <  2:4943915/93‑1 (MQ=255)

                                                    tCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAt                                     >  1:4772598/1‑93 (MQ=255)

                                                     cAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATa                                    <  2:3538841/93‑1 (MQ=255)

                                                      aGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAg                                   <  1:397191/93‑1 (MQ=255)

                                                      aGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAg                                   >  1:2825195/1‑93 (MQ=255)

                                                       gCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGc                                  >  2:3163780/1‑93 (MQ=255)

                                                         aTCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGccc                                <  2:4427506/93‑1 (MQ=255)

                                                          tCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCa                               >  2:5304288/1‑93 (MQ=255)

                                                          tCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCa                               <  2:3947352/93‑1 (MQ=255)

                                                          tCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCa                               >  1:4239715/1‑93 (MQ=255)

                                                            cATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAga                             <  1:1826872/93‑1 (MQ=255)

                                                            cATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAga                             <  2:4256040/93‑1 (MQ=255)

                                                            cATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAga                             <  1:4113416/93‑1 (MQ=255)

                                                             atatAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCGCCGTGATCCCCACTAACGTATGGCGATAGCCCAgac                            <  2:2488598/93‑1 (MQ=255)

                                                             atatAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgac                            <  1:2336943/93‑1 (MQ=255)

                                                             atatAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgac                            <  1:1618477/93‑1 (MQ=255)

                                                             atatAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgac                            <  1:3602428/93‑1 (MQ=255)

                                                              tataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacg                           >  2:1631519/1‑93 (MQ=255)

                                                              tataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacg                           >  2:4700172/1‑93 (MQ=255)

                                                              tataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacg                           >  2:2610608/1‑93 (MQ=255)

                                                              tataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacg                           >  1:4912094/1‑93 (MQ=255)

                                                               ataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacga                          <  2:77987/93‑1 (MQ=255)

                                                               ataGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAgacga                          >  2:5484170/1‑93 (MQ=255)

                                                                  gCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGt                       >  2:1722470/1‑93 (MQ=255)

                                                                    cATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTaa                     >  2:3635726/1‑93 (MQ=255)

                                                                    cATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTaa                     <  2:3462666/93‑1 (MQ=255)

                                                                    cATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTaa                     <  2:5182967/93‑1 (MQ=255)

                                                                     aTTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGACCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAg                    <  1:6235674/93‑1 (MQ=255)

                                                                      ttGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGa                   >  1:2795672/1‑93 (MQ=255)

                                                                       tGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGAc                  <  1:5373545/93‑1 (MQ=255)

                                                                        gCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACt                 <  1:5520817/93‑1 (MQ=255)

                                                                         cTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACtt                <  2:5069130/93‑1 (MQ=255)

                                                                          tGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTg               <  2:2357973/93‑1 (MQ=255)

                                                                           ggTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTgg              >  1:549334/1‑93 (MQ=255)

                                                                             tGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGt            >  2:578121/1‑93 (MQ=255)

                                                                             tGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGt            <  1:3706818/93‑1 (MQ=255)

                                                                              gATTGCAAAGAGGACTGAGTGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTa           >  2:6139773/1‑93 (MQ=255)

                                                                              gATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTa           >  2:2095494/1‑93 (MQ=255)

                                                                                ttGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAAc         >  2:4110667/1‑93 (MQ=255)

                                                                                   cAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCAt      <  2:5943730/93‑1 (MQ=255)

                                                                                   cAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCAt      <  2:293549/93‑1 (MQ=255)

                                                                                    aaaGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATc     >  1:5587173/1‑93 (MQ=255)

                                                                                     aaGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCg    >  2:1280106/1‑93 (MQ=255)

                                                                                     aaGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCg    >  1:1445946/1‑93 (MQ=255)

                                                                                      agagGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGa   >  1:4173578/1‑93 (MQ=255)

                                                                                      agagGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGa   >  1:6268838/1‑93 (MQ=255)

                                                                                      agagGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGa   <  1:3204739/93‑1 (MQ=255)

                                                                                       gaggACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAt  >  2:5569/1‑93 (MQ=255)

                                                                                       gaggACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAt  >  1:2846719/1‑93 (MQ=255)

ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAT  >  NC_000913/3937115‑3937294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGATTT  >  NC_000913/3937112‑3937296

TTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGG                                                                                             <  SRR4302533.2704284/94‑1 (MQ=60)

TTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGG                                                                                             <  SRR4302533.2266804/94‑1 (MQ=60)

 TCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGA                                                                                            <  SRR4302533.1131767/94‑1 (MQ=60)

 TCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGA                                                                                            <  SRR4302533.5607828/94‑1 (MQ=60)

  CATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAC                                                                                           <  SRR4302533.2673728/94‑1 (MQ=60)

  CATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAC                                                                                           <  SRR4302533.4198701/94‑1 (MQ=60)

  CATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAC                                                                                           <  SRR4302533.5057144/94‑1 (MQ=60)

  CATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGAC                                                                                           >  SRR4302533.2187543/1‑94 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          <  SRR4302533.4139972/94‑1 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          >  SRR4302533.4552684/1‑94 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          <  SRR4302533.1565979/94‑1 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          <  SRR4302533.5411785/94‑1 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          <  SRR4302533.5583157/94‑1 (MQ=60)

   ATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACT                                                                                          >  SRR4302533.5693594/1‑94 (MQ=60)

    TCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTG                                                                                         <  SRR4302533.3013230/94‑1 (MQ=60)

     CGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGA                                                                                        >  SRR4302533.5848025/1‑94 (MQ=60)

     CGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGA                                                                                        >  SRR4302533.49193/1‑94 (MQ=60)

     CGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGA                                                                                        >  SRR4302533.3677619/1‑94 (MQ=60)

      gccctaaacagcgaCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAG                                                                                       <  SRR4302533.1163088/80‑1 (MQ=60)

      GCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAG                                                                                       <  SRR4302533.101643/94‑1 (MQ=60)

      GCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAG                                                                                       >  SRR4302533.2142846/1‑94 (MQ=60)

        GGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCG                                                                                     <  SRR4302533.5810618/94‑1 (MQ=60)

        GGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCG                                                                                     >  SRR4302533.2396868/1‑94 (MQ=60)

        GGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCG                                                                                     <  SRR4302533.648600/94‑1 (MQ=60)

        GGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCG                                                                                     >  SRR4302533.2299290/1‑94 (MQ=60)

        GGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCG                                                                                     <  SRR4302533.3113052/94‑1 (MQ=60)

          TAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGAT                                                                                   <  SRR4302533.4857009/94‑1 (MQ=60)

           AAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATC                                                                                  <  SRR4302533.951127/94‑1 (MQ=60)

            AACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCA                                                                                 >  SRR4302533.522777/1‑94 (MQ=60)

            AACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCA                                                                                 >  SRR4302533.1588343/1‑94 (MQ=60)

            AACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCA                                                                                 >  SRR4302533.3484942/1‑94 (MQ=60)

            AACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCA                                                                                 >  SRR4302533.6041359/1‑94 (MQ=60)

             ACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCAT                                                                                <  SRR4302533.3562425/94‑1 (MQ=60)

                  GTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCC                                                                           <  SRR4302533.2386462/94‑1 (MQ=60)

                    CGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCA                                                                         <  SRR4302533.4828062/94‑1 (MQ=60)

                    CGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCA                                                                         >  SRR4302533.798672/1‑94 (MQ=60)

                    CGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCA                                                                         <  SRR4302533.606258/94‑1 (MQ=60)

                     GACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAA                                                                        >  SRR4302533.2150634/1‑94 (MQ=60)

                     GACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAA                                                                        <  SRR4302533.594385/94‑1 (MQ=60)

                      ACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAAT                                                                       >  SRR4302533.6162453/1‑94 (MQ=60)

                       CATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATA                                                                      >  SRR4302533.5024522/1‑94 (MQ=60)

                       CATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGAGATCATTAGCCCAATA                                                                      >  SRR4302533.5519271/1‑94 (MQ=60)

                        ATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAA                                                                     >  SRR4302533.3838890/1‑94 (MQ=60)

                          AGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATC                                                                   >  SRR4302533.3158557/1‑94 (MQ=60)

                          AGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATC                                                                   <  SRR4302533.1847488/94‑1 (MQ=60)

                           GCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCA                                                                  >  SRR4302533.6117731/1‑94 (MQ=60)

                            CCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCAC                                                                 >  SRR4302533.4698178/1‑94 (MQ=60)

                              ATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCG                                                               <  SRR4302533.5045161/94‑1 (MQ=60)

                              ATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCG                                                               <  SRR4302533.1082371/94‑1 (MQ=60)

                               TCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCCCCGT                                                              <  SRR4302533.5416099/94‑1 (MQ=60)

                               TCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGT                                                              <  SRR4302533.5416101/94‑1 (MQ=60)

                               TCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGT                                                              <  SRR4302533.6302709/94‑1 (MQ=60)

                               TCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGT                                                              >  SRR4302533.3908292/1‑94 (MQ=60)

                                CCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTG                                                             >  SRR4302533.2967352/1‑94 (MQ=60)

                                CCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTG                                                             <  SRR4302533.4898113/94‑1 (MQ=60)

                                CCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTG                                                             >  SRR4302533.3925413/1‑94 (MQ=60)

                                 CCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGA                                                            <  SRR4302533.2323696/94‑1 (MQ=60)

                                 CCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGA                                                            >  SRR4302533.2544896/1‑94 (MQ=60)

                                 CCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGA                                                            >  SRR4302533.5865355/1‑94 (MQ=60)

                                 CATAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGA                                                            <  SRR4302533.2541059/94‑1 (MQ=60)

                                    AATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCC                                                         <  SRR4302533.6185895/94‑1 (MQ=60)

                                     ATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGTGCGATCATTAGCCCAATAATCACCGTGATCCC                                                        >  SRR4302533.254650/1‑94 (MQ=60)

                                     ATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCC                                                        >  SRR4302533.2520130/1‑94 (MQ=60)

                                     ATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCC                                                        <  SRR4302533.1729695/94‑1 (MQ=60)

                                     ATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCC                                                        >  SRR4302533.326462/1‑94 (MQ=60)

                                     ATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCC                                                        >  SRR4302533.2084723/1‑94 (MQ=60)

                                      TATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCC                                                       >  SRR4302533.6016356/1‑94 (MQ=60)

                                       ATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCA                                                      >  SRR4302533.4086275/1‑94 (MQ=60)

                                       ATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCA                                                      >  SRR4302533.3520055/1‑94 (MQ=60)

                                         AAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACT                                                    <  SRR4302533.4894542/94‑1 (MQ=60)

                                         AAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACT                                                    >  SRR4302533.2390770/1‑94 (MQ=60)

                                          AACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTA                                                   <  SRR4302533.883818/94‑1 (MQ=60)

                                            CAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAAC                                                 >  SRR4302533.5797905/1‑94 (MQ=60)

                                             AACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACG                                                >  SRR4302533.422162/1‑94 (MQ=60)

                                             AACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACG                                                >  SRR4302533.3202499/1‑94 (MQ=60)

                                               CGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTA                                              >  SRR4302533.1481798/1‑94 (MQ=60)

                                               CGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTA                                              <  SRR4302533.6247313/94‑1 (MQ=60)

                                                GGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCTCCGTGATCCCCACTAACGTAT                                             <  SRR4302533.3445626/94‑1 (MQ=60)

                                                GGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTAT                                             <  SRR4302533.1432556/94‑1 (MQ=60)

                                                GGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTAT                                             <  SRR4302533.4893614/94‑1 (MQ=60)

                                                 GCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATG                                            <  SRR4302533.3574548/94‑1 (MQ=60)

                                                 GCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATG                                            <  SRR4302533.5794150/94‑1 (MQ=60)

                                                   AAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGC                                          <  SRR4302533.616620/94‑1 (MQ=60)

                                                    AGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCG                                         >  SRR4302533.5602634/1‑94 (MQ=60)

                                                    AGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCG                                         >  SRR4302533.2380777/1‑94 (MQ=60)

                                                      ATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAT                                       >  SRR4302533.4367169/1‑94 (MQ=60)

                                                      ATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAT                                       <  SRR4302533.214002/94‑1 (MQ=60)

                                                      ATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAT                                       >  SRR4302533.4801521/1‑94 (MQ=60)

                                                      ATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAT                                       >  SRR4302533.3431587/1‑94 (MQ=60)

                                                      ATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGAT                                       >  SRR4302533.3409200/1‑94 (MQ=60)

                                                       TCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATA                                      <  SRR4302533.3364610/94‑1 (MQ=60)

                                                       TCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATA                                      <  SRR4302533.4351980/94‑1 (MQ=60)

                                                       TCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATA                                      <  SRR4302533.1520808/94‑1 (MQ=60)

                                                        CAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAG                                     >  SRR4302533.2842580/1‑94 (MQ=60)

                                                         AGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGC                                    <  SRR4302533.399592/94‑1 (MQ=60)

                                                          GCATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCC                                   <  SRR4302533.4079301/94‑1 (MQ=60)

                                                           CATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCC                                  >  SRR4302533.2829205/1‑94 (MQ=60)

                                                            ATCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCA                                 >  SRR4302533.4265320/1‑94 (MQ=60)

                                                             TCCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAG                                >  SRR4302533.4208539/1‑94 (MQ=60)

                                                              CCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGC                               >  SRR4302533.414774/1‑94 (MQ=60)

                                                              CCATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGA                               >  SRR4302533.346845/1‑94 (MQ=60)

                                                               CATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGAC                              <  SRR4302533.4138414/94‑1 (MQ=60)

                                                               CATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGAC                              <  SRR4302533.1838030/94‑1 (MQ=60)

                                                                ATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACG                             <  SRR4302533.1628282/94‑1 (MQ=60)

                                                                ATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACG                             >  SRR4302533.1400249/1‑94 (MQ=60)

                                                                ATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACG                             >  SRR4302533.4941848/1‑94 (MQ=60)

                                                                ATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACG                             <  SRR4302533.3624597/94‑1 (MQ=60)

                                                                ATATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACG                             <  SRR4302533.2351408/94‑1 (MQ=60)

                                                                  ATAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGAC                           >  SRR4302533.5938087/1‑94 (MQ=60)

                                                                   TAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACG                          <  SRR4302533.2167871/94‑1 (MQ=60)

                                                                   TAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACG                          >  SRR4302533.1626702/1‑94 (MQ=60)

                                                                   TAGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACG                          <  SRR4302533.499482/94‑1 (MQ=60)

                                                                    AGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGT                         <  SRR4302533.5843813/94‑1 (MQ=60)

                                                                    AGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGT                         >  SRR4302533.1701709/1‑94 (MQ=60)

                                                                    AGCCATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGT                         <  SRR4302533.2645381/94‑1 (MQ=60)

                                                                       CATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAG                      >  SRR4302533.5319239/1‑94 (MQ=60)

                                                                        ATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGA                     >  SRR4302533.2812865/1‑94 (MQ=60)

                                                                        ATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGA                     >  SRR4302533.4904197/1‑94 (MQ=60)

                                                                        ATTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGACCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGA                     <  SRR4302533.6273017/94‑1 (MQ=60)

                                                                         TTGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGAC                    <  SRR4302533.2861391/94‑1 (MQ=60)

                                                                          TGCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACT                   <  SRR4302533.5405833/94‑1 (MQ=60)

                                                                           GCTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTT                  <  SRR4302533.5554149/94‑1 (MQ=60)

                                                                            CTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTG                 <  SRR4302533.3234420/94‑1 (MQ=60)

                                                                            CTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTG                 <  SRR4302533.2339920/94‑1 (MQ=60)

                                                                            CTGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTG                 >  SRR4302533.5552330/1‑94 (MQ=60)

                                                                             TGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGG                <  SRR4302533.262831/94‑1 (MQ=60)

                                                                             TGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGG                >  SRR4302533.552593/1‑94 (MQ=60)

                                                                             TGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGG                <  SRR4302533.813709/94‑1 (MQ=60)

                                                                             TGGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGG                <  SRR4302533.3649105/94‑1 (MQ=60)

                                                                              GGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGG               <  SRR4302533.2004183/94‑1 (MQ=60)

                                                                              GGTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGG               <  SRR4302533.671678/94‑1 (MQ=60)

                                                                               GTGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGT              <  SRR4302533.3951397/94‑1 (MQ=60)

                                                                                TGATTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTA             <  SRR4302533.283611/94‑1 (MQ=60)

                                                                                TGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTA             <  SRR4302533.3729585/94‑1 (MQ=60)

                                                                                   TTGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACC          >  SRR4302533.2244844/1‑94 (MQ=60)

                                                                                    TGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCA         <  SRR4302533.4632014/94‑1 (MQ=60)

                                                                                    TGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCA         >  SRR4302533.2490390/1‑94 (MQ=60)

                                                                                    TGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCA         >  SRR4302533.2221694/1‑94 (MQ=60)

                                                                                    TGCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCA         >  SRR4302533.5286979/1‑94 (MQ=60)

                                                                                     GCAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCAT        >  SRR4302533.5182267/1‑94 (MQ=60)

                                                                                      CAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATC       >  SRR4302533.5620929/1‑94 (MQ=60)

                                                                                      CAAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATC       <  SRR4302533.5448421/94‑1 (MQ=60)

                                                                                       AAAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCG      >  SRR4302533.1454670/1‑94 (MQ=60)

                                                                                        AAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGA     >  SRR4302533.4198868/1‑94 (MQ=60)

                                                                                        AAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGA     >  SRR4302533.6306296/1‑94 (MQ=60)

                                                                                        AAGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGA     >  SRR4302533.5202697/1‑94 (MQ=60)

                                                                                         AGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAT    <  SRR4302533.3224470/94‑1 (MQ=60)

                                                                                         AGAGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGAT    >  SRR4302533.2864241/1‑94 (MQ=60)

                                                                                           AGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGATTT  <  SRR4302533.3897332/94‑1 (MQ=60)

                                                                                           AGGACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGATTT  >  SRR4302533.3043937/1‑94 (MQ=60)

TTCATCGCGGTAAACTGCGTCGACATAGCCATCCCTAATATAAACAACGGCAAGATCAGCATCCATATAGCCATTGCTGGTGATTGCAAAGAGAACTGAGCGATCATTAGCCCAATAATCACCGTGATCCCCACTAACGTATGGCGATAGCCCAGACGACGTAAGACTTGGGTAACCATCGATTT  >  NC_000913/3937112‑3937296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`