Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,948,221	A→G	A13A (GCA→GCG)	ilvG →	acetolactate synthase II large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,948,221	0	A	G	100.0%	694.2 / NA	216	A13A (GCA→GCG)	ilvG	acetolactate synthase II large subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (104/112); total (104/112)

Only 100 of 224 total aligned reads displayed.

TGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCT  >  NC_000913/3948131‑3948310

tGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGcgca                                                                                         >  1:3597001/1‑93 (MQ=255)

tGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGcgca                                                                                         >  1:5430115/1‑93 (MQ=255)

   aTAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAggg                                                                                      <  1:340522/93‑1 (MQ=255)

    tAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgt                                                                                     >  2:3427961/1‑93 (MQ=255)

     aaCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgtg                                                                                    <  2:5209490/93‑1 (MQ=255)

     aaCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgtg                                                                                    >  1:6226583/1‑93 (MQ=255)

      aCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgtgt                                                                                   <  2:792071/93‑1 (MQ=255)

      aCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgtgt                                                                                   >  1:4391764/1‑93 (MQ=255)

      aCAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGgtgt                                                                                   >  1:4861343/1‑93 (MQ=255)

        aGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGa                                                                                 <  1:4165122/93‑1 (MQ=255)

         gCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGaa                                                                                >  1:2200206/1‑93 (MQ=255)

         gCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGaa                                                                                <  1:525556/93‑1 (MQ=255)

         gCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGaa                                                                                <  2:307963/93‑1 (MQ=255)

           acaAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAaca                                                                              <  1:2590416/93‑1 (MQ=255)

            caAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAacac                                                                             >  1:5947423/1‑93 (MQ=255)

             aaaaTTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACAcc                                                                            >  1:4232634/1‑93 (MQ=255)

              aaaTTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCg                                                                           <  2:5636644/93‑1 (MQ=255)

              aaaTTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCg                                                                           >  1:3366324/1‑93 (MQ=255)

                aTTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGtt                                                                         <  1:5202558/93‑1 (MQ=255)

                  tCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGtttt                                                                       >  1:2476595/1‑93 (MQ=255)

                    tGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCg                                                                     >  2:4313969/1‑93 (MQ=255)

                     gTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCgg                                                                    >  2:83785/1‑93 (MQ=255)

                      tttCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGt                                                                   <  1:647923/93‑1 (MQ=255)

                       ttCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGtt                                                                  >  1:110597/1‑93 (MQ=255)

                        tctcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTa                                                                 >  2:2436471/1‑93 (MQ=255)

                         ctcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTAt                                                                >  2:2445151/1‑93 (MQ=255)

                         ctcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTAt                                                                >  2:2973435/1‑93 (MQ=255)

                          tcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATc                                                               <  1:3329067/93‑1 (MQ=255)

                          tcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATc                                                               >  2:1145167/1‑93 (MQ=255)

                          tcAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATc                                                               <  1:2034902/93‑1 (MQ=255)

                           cAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATcc                                                              <  1:1364870/93‑1 (MQ=255)

                           cAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATcc                                                              <  1:513947/93‑1 (MQ=255)

                           cAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATcc                                                              <  2:5000163/93‑1 (MQ=255)

                            aaGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCg                                                             >  2:4001345/1‑93 (MQ=255)

                            aaGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCg                                                             <  1:1446975/93‑1 (MQ=255)

                             aGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCgg                                                            >  2:3644069/1‑93 (MQ=255)

                             aGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGCGTGAACACCGTTTTCGGTTATCCgg                                                            >  2:4509648/1‑93 (MQ=255)

                              gATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCATGGTGTGAACACCGTTTTCCGTTATCCggg                                                           >  1:2859317/1‑93 (MQ=255)

                              gATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCggg                                                           <  1:4003525/93‑1 (MQ=255)

                              gATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCggg                                                           >  1:3143838/1‑93 (MQ=255)

                              gATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCggg                                                           >  2:1370326/1‑93 (MQ=255)

                               aTTCAGGACGGGGATCTATCTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACTGTTTTCGGTTATCCGGGt                                                          <  1:5731988/93‑1 (MQ=255)

                                ttCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTg                                                         <  2:3570946/93‑1 (MQ=255)

                                  cAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGgc                                                       <  1:3183218/93‑1 (MQ=255)

                                     gACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCa                                                    >  2:3334466/1‑93 (MQ=255)

                                     gACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCa                                                    >  2:1853728/1‑93 (MQ=255)

                                     gACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGACGCa                                                    >  1:4688917/1‑93 (MQ=255)

                                      aCGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCaa                                                   <  1:4759876/93‑1 (MQ=255)

                                      aCGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCaa                                                   >  1:2701513/1‑93 (MQ=255)

                                        ggggAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAAtt                                                 >  1:3832214/1‑93 (MQ=255)

                                         gggAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTa                                                <  1:1513388/93‑1 (MQ=255)

                                         gggAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTa                                                <  2:2130440/93‑1 (MQ=255)

                                          ggAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAt                                               <  1:420178/93‑1 (MQ=255)

                                          ggAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAt                                               <  2:6178751/93‑1 (MQ=255)

                                           gAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATg                                              >  2:2894624/1‑93 (MQ=255)

                                           gAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATg                                              >  2:2655779/1‑93 (MQ=255)

                                           gAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATg                                              >  2:5812920/1‑93 (MQ=255)

                                            aactaactATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGc                                             <  1:2085299/93‑1 (MQ=255)

                                             actaactaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACCCCGTTTTCGGTTATCCGGGTGGCGCAATTATGcc                                            <  1:4079606/93‑1 (MQ=255)

                                             actaactaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGCTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGcc                                            <  1:4446252/93‑1 (MQ=255)

                                              ctaactaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCg                                           >  2:5262195/1‑93 (MQ=255)

                                               taactaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCgg                                          <  2:5134326/93‑1 (MQ=255)

                                                aactaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGt                                         <  2:699183/93‑1 (MQ=255)

                                                 actaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGtt                                        >  2:4609679/1‑93 (MQ=255)

                                                  ctaTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGttt                                       >  2:712115/1‑93 (MQ=255)

                                                    aTGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTAc                                     <  2:1031181/93‑1 (MQ=255)

                                                     tGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACg                                    >  2:3832098/1‑93 (MQ=255)

                                                      gAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGa                                   >  1:3469939/1‑93 (MQ=255)

                                                        aTGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATg                                 <  2:2697060/93‑1 (MQ=255)

                                                        aTGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATg                                 <  1:702713/93‑1 (MQ=255)

                                                         tGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGc                                <  1:2491370/93‑1 (MQ=255)

                                                         tGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGc                                <  1:1725732/93‑1 (MQ=255)

                                                          ggCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCa                               >  1:5546429/1‑93 (MQ=255)

                                                          ggCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCa                               <  2:5541026/93‑1 (MQ=255)

                                                              cacaGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGt                           >  2:2742535/1‑93 (MQ=255)

                                                               acaGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTa                          <  1:385893/93‑1 (MQ=255)

                                                               acaGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTa                          <  1:1819682/93‑1 (MQ=255)

                                                                caGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTAt                         >  2:281898/1‑93 (MQ=255)

                                                                 aGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATg                        <  2:4211437/93‑1 (MQ=255)

                                                                 aGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATg                        <  1:5229100/93‑1 (MQ=255)

                                                                   tgggtggTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAc                      <  2:4927942/93‑1 (MQ=255)

                                                                     ggtggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcgg                    <  1:4843181/93‑1 (MQ=255)

                                                                     ggtggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcgg                    <  2:4395225/93‑1 (MQ=255)

                                                                     ggtggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcgg                    <  2:2254703/93‑1 (MQ=255)

                                                                      gtggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcggc                   <  1:2498528/93‑1 (MQ=255)

                                                                      gtggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcggc                   <  1:3986953/93‑1 (MQ=255)

                                                                        ggtACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGAcggcgg                 >  1:6058505/1‑93 (MQ=255)

                                                                            cATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGGTTACGATGCATTGTATGACGGCGGGGTg             >  2:3142750/1‑93 (MQ=255)

                                                                             aTGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTgg            >  2:470316/1‑93 (MQ=255)

                                                                             aTGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTgg            >  1:293619/1‑93 (MQ=255)

                                                                             aTGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTgg            <  1:1743650/93‑1 (MQ=255)

                                                                             aTGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTgg            <  2:5744766/93‑1 (MQ=255)

                                                                              tGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGa           <  1:5367296/93‑1 (MQ=255)

                                                                              tGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGa           >  1:1881293/1‑93 (MQ=255)

                                                                               gCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAg          >  1:1072979/1‑93 (MQ=255)

                                                                                  ttGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCAc       >  1:3009412/1‑93 (MQ=255)

                                                                                   tGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACt      <  1:745944/93‑1 (MQ=255)

                                                                                      gggCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGc   <  1:2963856/93‑1 (MQ=255)

                                                                                      gggCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGc   >  2:1539694/1‑93 (MQ=255)

                                                                                       ggCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCt  <  2:5892630/93‑1 (MQ=255)

TGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCT  >  NC_000913/3948131‑3948310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGC  >  NC_000913/3948128‑3948314

CAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCG                                                                                               >  SRR4302533.1621271/1‑94 (MQ=60)

CAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCG                                                                                               >  SRR4302533.5529153/1‑94 (MQ=60)

 AATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGC                                                                                              >  SRR4302533.1427588/1‑94 (MQ=60)

  ATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCA                                                                                             >  SRR4302533.3619134/1‑94 (MQ=60)

  ATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCA                                                                                             >  SRR4302533.5462819/1‑94 (MQ=60)

   TGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAG                                                                                            >  SRR4302533.1504004/1‑94 (MQ=60)

   TGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAG                                                                                            <  SRR4302533.57437/94‑1 (MQ=60)

    GAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGG                                                                                           >  SRR4302533.4213374/1‑94 (MQ=60)

     AATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGG                                                                                          >  SRR4302533.6151229/1‑94 (MQ=60)

      ATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGT                                                                                         <  SRR4302533.342601/94‑1 (MQ=60)

       TAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTG                                                                                        >  SRR4302533.6263886/1‑94 (MQ=60)

       TAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGCGGTACATGCGTTGCGGGCGCAGGGTG                                                                                        >  SRR4302533.995724/1‑94 (MQ=60)

        AACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGT                                                                                       >  SRR4302533.4890808/1‑94 (MQ=60)

        AACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGT                                                                                       >  SRR4302533.4418275/1‑94 (MQ=60)

         ACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTG                                                                                      >  SRR4302533.4124867/1‑94 (MQ=60)

          CAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACCGTGGGCGGTACATGCGTTGCGGGCGCAGGGTGTGA                                                                                     >  SRR4302533.5060480/1‑94 (MQ=60)

           AGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAA                                                                                    <  SRR4302533.4190358/94‑1 (MQ=60)

           AGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAA                                                                                    >  SRR4302533.2213760/1‑94 (MQ=60)

            GCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAAC                                                                                   <  SRR4302533.528670/94‑1 (MQ=60)

             CACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCGCAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACA                                                                                  <  SRR4302533.714392/94‑1 (MQ=60)

             CACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACA                                                                                  <  SRR4302533.73055/94‑1 (MQ=60)

              ACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACAC                                                                                 <  SRR4302533.2606335/94‑1 (MQ=60)

              ACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACAC                                                                                 >  SRR4302533.5983396/1‑94 (MQ=60)

               CAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACC                                                                                >  SRR4302533.4258215/1‑94 (MQ=60)

               CAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACC                                                                                <  SRR4302533.3412875/94‑1 (MQ=60)

                AAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCG                                                                               >  SRR4302533.3387117/1‑94 (MQ=60)

                AAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCG                                                                               >  SRR4302533.5349356/1‑94 (MQ=60)

                  AATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTT                                                                             <  SRR4302533.5990474/94‑1 (MQ=60)

                   ATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTT                                                                            >  SRR4302533.3101568/1‑94 (MQ=60)

                   ATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTT                                                                            <  SRR4302533.5233912/94‑1 (MQ=60)

                    TTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTT                                                                           >  SRR4302533.2491870/1‑94 (MQ=60)

                    TTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTT                                                                           >  SRR4302533.2496954/1‑94 (MQ=60)

                      CTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCG                                                                         >  SRR4302533.4083174/1‑94 (MQ=60)

                      CTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCG                                                                         <  SRR4302533.43073/94‑1 (MQ=60)

                      CTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCG                                                                         <  SRR4302533.1703528/94‑1 (MQ=60)

                         TTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTT                                                                      <  SRR4302533.651742/94‑1 (MQ=60)

                         TTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTT                                                                      >  SRR4302533.111298/1‑94 (MQ=60)

                          TTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTA                                                                     >  SRR4302533.2052304/1‑94 (MQ=60)

                          TTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTA                                                                     >  SRR4302533.2805494/1‑94 (MQ=60)

                          TTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTA                                                                     >  SRR4302533.2805496/1‑94 (MQ=60)

                             TCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCC                                                                  <  SRR4302533.3349643/94‑1 (MQ=60)

                             TCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCC                                                                  <  SRR4302533.1996028/94‑1 (MQ=60)

                             TCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCC                                                                  <  SRR4302533.2047454/94‑1 (MQ=60)

                             TCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGGGTGAACACCGTTTTCGGTTATCC                                                                  >  SRR4302533.5405429/1‑94 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 >  SRR4302533.4647996/1‑94 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 <  SRR4302533.517002/94‑1 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 <  SRR4302533.1373070/94‑1 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 >  SRR4302533.4725890/1‑94 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 <  SRR4302533.3360385/94‑1 (MQ=60)

                              CAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACAGGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCG                                                                 <  SRR4302533.3051431/94‑1 (MQ=60)

                               AAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTTTCCGG                                                                >  SRR4302533.4882350/1‑94 (MQ=60)

                               AAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGG                                                                <  SRR4302533.1743573/94‑1 (MQ=60)

                               AAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGG                                                                <  SRR4302533.1455706/94‑1 (MQ=60)

                                AGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCATGGTGTGAACACCGTTTTCCGTTATCCGGG                                                               >  SRR4302533.2876907/1‑94 (MQ=60)

                                AGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGG                                                               >  SRR4302533.5242045/1‑94 (MQ=60)

                                AGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGG                                                               <  SRR4302533.1239826/94‑1 (MQ=60)

                                AGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGG                                                               >  SRR4302533.3163164/1‑94 (MQ=60)

                                 GATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGT                                                              <  SRR4302533.4027901/94‑1 (MQ=60)

                                 GATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGG                                                              >  SRR4302533.5915470/1‑94 (MQ=60)

                                  ATTCAGGACGGGGATCTATCTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACTGTTTTCGGTTATCCGGGTG                                                             <  SRR4302533.5766593/94‑1 (MQ=60)

                                  ATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTG                                                             <  SRR4302533.481620/94‑1 (MQ=60)

                                  ATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTG                                                             >  SRR4302533.2923172/1‑94 (MQ=60)

                                   TTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGG                                                            >  SRR4302533.2492624/1‑94 (MQ=60)

                                     CAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCG                                                          <  SRR4302533.3202800/94‑1 (MQ=60)

                                     CAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCG                                                          >  SRR4302533.3978064/1‑94 (MQ=60)

                                      AGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGC                                                         >  SRR4302533.3940351/1‑94 (MQ=60)

                                       GGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGACGCA                                                        >  SRR4302533.4717341/1‑94 (MQ=60)

                                        GACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAA                                                       >  SRR4302533.1885973/1‑94 (MQ=60)

                                        GACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAA                                                       >  SRR4302533.2718087/1‑94 (MQ=60)

                                         ACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAAT                                                      <  SRR4302533.4788752/94‑1 (MQ=60)

                                          CGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATT                                                     >  SRR4302533.3855638/1‑94 (MQ=60)

                                           GGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTA                                                    >  SRR4302533.2507210/1‑94 (MQ=60)

                                            GGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAT                                                   <  SRR4302533.3188435/94‑1 (MQ=60)

                                            GGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAT                                                   >  SRR4302533.4025808/1‑94 (MQ=60)

                                            GGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAT                                                   >  SRR4302533.556122/1‑94 (MQ=60)

                                            GGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAT                                                   <  SRR4302533.1522507/94‑1 (MQ=60)

                                            GGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTAT                                                   <  SRR4302533.2428540/94‑1 (MQ=60)

                                             GGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATG                                                  <  SRR4302533.3464089/94‑1 (MQ=60)

                                             GGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATG                                                  <  SRR4302533.422693/94‑1 (MQ=60)

                                              GAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGC                                                 >  SRR4302533.3014449/1‑94 (MQ=60)

                                               AACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCC                                                <  SRR4302533.4447287/94‑1 (MQ=60)

                                               AACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCC                                                <  SRR4302533.2098138/94‑1 (MQ=60)

                                                ACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACCCCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCG                                               <  SRR4302533.4104440/94‑1 (MQ=60)

                                                ACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCG                                               <  SRR4302533.2034407/94‑1 (MQ=60)

                                                ACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCG                                               <  SRR4302533.2698832/94‑1 (MQ=60)

                                                ACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGCTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCG                                               <  SRR4302533.4473144/94‑1 (MQ=60)

                                                  TAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGT                                             <  SRR4302533.3882393/94‑1 (MQ=60)

                                                  TAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGT                                             >  SRR4302533.3782670/1‑94 (MQ=60)

                                                    ACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTT                                           >  SRR4302533.49147/1‑94 (MQ=60)

                                                        TGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGTTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGA                                       <  SRR4302533.4233780/94‑1 (MQ=60)

                                                        TGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGA                                       >  SRR4302533.3491359/1‑94 (MQ=60)

                                                          AATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATG                                     >  SRR4302533.1822654/1‑94 (MQ=60)

                                                           ATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGC                                    <  SRR4302533.706869/94‑1 (MQ=60)

                                                           ATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGC                                    <  SRR4302533.3208996/94‑1 (MQ=60)

                                                            TGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCA                                   <  SRR4302533.3564442/94‑1 (MQ=60)

                                                            TGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCA                                   <  SRR4302533.2506715/94‑1 (MQ=60)

                                                            TGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCA                                   >  SRR4302533.5579928/1‑94 (MQ=60)

                                                            TGGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCA                                   <  SRR4302533.1736205/94‑1 (MQ=60)

                                                             GGCGCACAGTGGGTGGTACATGCGTTGCGGGCGCAGCGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCAT                                  <  SRR4302533.5269301/94‑1 (MQ=60)

                                                                GCACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGT                               >  SRR4302533.4226995/1‑94 (MQ=60)

                                                                  ACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTAT                             <  SRR4302533.388222/94‑1 (MQ=60)

                                                                  ACAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTAT                             <  SRR4302533.1830798/94‑1 (MQ=60)

                                                                   CAGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATG                            <  SRR4302533.2972218/94‑1 (MQ=60)

                                                                    AGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGA                           <  SRR4302533.5260555/94‑1 (MQ=60)

                                                                    AGTGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGA                           <  SRR4302533.4580021/94‑1 (MQ=60)

                                                                      TGGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACG                         <  SRR4302533.6337140/94‑1 (MQ=60)

                                                                       GGGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGG                        >  SRR4302533.1726609/1‑94 (MQ=60)

                                                                        GGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGC                       <  SRR4302533.1088953/94‑1 (MQ=60)

                                                                        GGTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGC                       <  SRR4302533.4872536/94‑1 (MQ=60)

                                                                         GTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCG                      <  SRR4302533.4011237/94‑1 (MQ=60)

                                                                         GTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCG                      >  SRR4302533.5365470/1‑94 (MQ=60)

                                                                         GTGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCG                      <  SRR4302533.2513942/94‑1 (MQ=60)

                                                                          TGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCTTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGG                     <  SRR4302533.2549787/94‑1 (MQ=60)

                                                                          TGGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGG                     >  SRR4302533.6095185/1‑94 (MQ=60)

                                                                           GGTACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGC                    <  SRR4302533.1407202/94‑1 (MQ=60)

                                                                              ACATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTG                 >  SRR4302533.5891931/1‑94 (MQ=60)

                                                                               CATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGG                >  SRR4302533.295412/1‑94 (MQ=60)

                                                                                ATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGA               <  SRR4302533.1754252/94‑1 (MQ=60)

                                                                                ATGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGA               >  SRR4302533.1892749/1‑94 (MQ=60)

                                                                                 TGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAG              <  SRR4302533.5186071/94‑1 (MQ=60)

                                                                                 TGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAG              >  SRR4302533.1079306/1‑94 (MQ=60)

                                                                                 TGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAG              <  SRR4302533.5399541/94‑1 (MQ=60)

                                                                                 TGCGTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAG              <  SRR4302533.2112171/94‑1 (MQ=60)

                                                                                    GTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCAC           >  SRR4302533.3027825/1‑94 (MQ=60)

                                                                                    GTTGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAACTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCAC           <  SRR4302533.3395077/94‑1 (MQ=60)

                                                                                      TGCGGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTT         <  SRR4302533.750334/94‑1 (MQ=60)

                                                                                         GGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCT      <  SRR4302533.670755/94‑1 (MQ=60)

                                                                                         GGGCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCT      <  SRR4302533.2981975/94‑1 (MQ=60)

                                                                                           GCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTAT    >  SRR4302533.6051076/1‑94 (MQ=60)

                                                                                           GCGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTAT    <  SRR4302533.3330151/94‑1 (MQ=60)

                                                                                            CGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGGGGCGTGGAGCACTTGCTATG   >  SRR4302533.2082248/1‑94 (MQ=60)

                                                                                            CGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATG   <  SRR4302533.2458013/94‑1 (MQ=60)

                                                                                            CGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATG   >  SRR4302533.5749157/1‑94 (MQ=60)

                                                                                            CGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATG   <  SRR4302533.701431/94‑1 (MQ=60)

                                                                                            CGCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATG   <  SRR4302533.4739050/94‑1 (MQ=60)

                                                                                             GCAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGC  <  SRR4302533.5021200/94‑1 (MQ=60)

CAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGGCACAGGGTGTGAACACCGTTTTCGGTTATCCGGGTGGCGCAATTATGCCGGTTTACGATGCATTGTATGACGGCGGCGTGGAGCACTTGCTATGC  >  NC_000913/3948128‑3948314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`