Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,518,280	T→C	S437G (AGT→GGT)	yjhF ←	putative transport system permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,518,280	0	T	C	99.6%	787.5 / NA	242	S437G (AGT→GGT)	yjhF	putative transport system permease
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (109/132); minor base A (0/1); total (109/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

AATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGG  >  NC_000913/4518190‑4518369

  ttATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATg                                                                                       <  1:3205070/93‑1 (MQ=255)

     tGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGAt                                                                                    <  2:721410/93‑1 (MQ=255)

      gggggACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATa                                                                                   <  2:5420623/93‑1 (MQ=255)

          gACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAagag                                                                               <  2:1368063/93‑1 (MQ=255)

                gAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGa                                                                         <  2:1805721/93‑1 (MQ=255)

                gAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGa                                                                         >  2:308270/1‑93 (MQ=255)

                  acaacaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACa                                                                       <  1:2387355/93‑1 (MQ=255)

                   caacaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACAt                                                                      >  2:5465029/1‑93 (MQ=255)

                    aacaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATc                                                                     <  2:5297998/93‑1 (MQ=255)

                    aacaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATc                                                                     <  2:1001106/93‑1 (MQ=255)

                     acaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCa                                                                    >  2:2358952/1‑93 (MQ=255)

                     acaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCa                                                                    <  2:1739458/93‑1 (MQ=255)

                     acaGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCa                                                                    >  2:2851997/1‑93 (MQ=255)

                      caGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAg                                                                   >  1:1009209/1‑93 (MQ=255)

                      caGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGGGGGGACATCac                                                                   >  2:359174/1‑92 (MQ=255)

                       aGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGc                                                                  <  2:6146056/93‑1 (MQ=255)

                        ggTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCg                                                                 >  2:5585571/1‑93 (MQ=255)

                          gtGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTc                                                               <  2:6078799/92‑1 (MQ=255)

                           tGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATATAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTcc                                                              <  1:3379310/93‑1 (MQ=255)

                            ggCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCa                                                             <  1:3836964/93‑1 (MQ=255)

                            ggCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCa                                                             <  1:469752/93‑1 (MQ=255)

                             gCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAg                                                            >  2:5314543/1‑93 (MQ=255)

                             gCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAg                                                            <  1:4513313/93‑1 (MQ=255)

                             gCTCACTGGCAAGAACCCGATTATAAAACTAAGCTAGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAg                                                            >  2:2694746/1‑93 (MQ=255)

                              cTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAgg                                                           <  2:5899844/93‑1 (MQ=255)

                              cTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAgg                                                           >  2:4365686/1‑93 (MQ=255)

                               tCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGa                                                          <  2:3516930/93‑1 (MQ=255)

                               tCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGa                                                          >  2:3453897/1‑93 (MQ=255)

                               tCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGa                                                          <  2:2457186/93‑1 (MQ=255)

                                cACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGAt                                                         <  2:2253086/93‑1 (MQ=255)

                                cACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGAt                                                         <  1:3765101/93‑1 (MQ=255)

                                cACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGAt                                                         >  1:1591245/1‑93 (MQ=255)

                                 aCTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTTCAGGATa                                                        >  1:5214807/1‑93 (MQ=255)

                                   tGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCGGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATacc                                                      >  1:1419067/1‑91 (MQ=255)

                                   tGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAAc                                                      >  1:1163313/1‑93 (MQ=255)

                                   tGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAAc                                                      >  2:6016086/1‑93 (MQ=255)

                                      cAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGc                                                   <  1:2715221/93‑1 (MQ=255)

                                        aGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGt                                                 >  2:3744012/1‑93 (MQ=255)

                                        aGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGt                                                 <  1:5777252/93‑1 (MQ=255)

                                        aGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGGGTGGACATCAGCGTCCAGGATAACAGCGt                                                 >  2:4088896/1‑93 (MQ=255)

                                          aaCCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACGTCAGCGTCCAGGATAACAGCGttt                                               >  2:6184526/1‑93 (MQ=255)

                                          aaCCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGttt                                               >  1:3645656/1‑93 (MQ=255)

                                           aCCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTc                                              >  2:1844290/1‑93 (MQ=255)

                                            cccGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCt                                             >  2:3631606/1‑93 (MQ=255)

                                            cccGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCt                                             <  2:1503637/93‑1 (MQ=255)

                                             ccGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCtt                                            <  1:1881169/93‑1 (MQ=255)

                                             ccGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCtt                                            >  2:331448/1‑93 (MQ=255)

                                             ccGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCtt                                            <  1:597565/93‑1 (MQ=255)

                                              cGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCttt                                           <  1:5524860/93‑1 (MQ=255)

                                              cGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCttt                                           <  1:4304087/93‑1 (MQ=255)

                                               gATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtg                                          <  2:77588/93‑1 (MQ=255)

                                               gATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtg                                          >  2:5970422/1‑93 (MQ=255)

                                               gATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtg                                          <  1:5467510/93‑1 (MQ=255)

                                                 ttATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggt                                        <  2:5693988/93‑1 (MQ=255)

                                                  tataAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggtg                                       <  2:2955304/93‑1 (MQ=255)

                                                  tataAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggtg                                       <  2:4586994/93‑1 (MQ=255)

                                                  tataAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggtg                                       >  2:3381920/1‑93 (MQ=255)

                                                   ataAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggtgg                                      >  2:898151/1‑93 (MQ=255)

                                                   ataAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTtggtgg                                      <  1:1805159/93‑1 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     <  2:4695599/93‑1 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     <  1:2094358/93‑1 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     >  1:4909215/1‑93 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     <  2:1548864/93‑1 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     >  1:2226428/1‑93 (MQ=255)

                                                    taAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTggtggt                                     >  2:807290/1‑93 (MQ=255)

                                                     aaaaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTc                                    <  1:5104565/93‑1 (MQ=255)

                                                     aaaaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTc                                    >  2:2915910/1‑93 (MQ=255)

                                                      aaaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCa                                   <  2:4902511/93‑1 (MQ=255)

                                                      aaaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCa                                   <  1:5818057/93‑1 (MQ=255)

                                                      aaaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCa                                   >  2:1210582/1‑93 (MQ=255)

                                                       aaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAg                                  >  1:5928551/1‑93 (MQ=255)

                                                       aaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAg                                  <  1:3100165/93‑1 (MQ=255)

                                                       aaCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAg                                  <  2:1979416/93‑1 (MQ=255)

                                                        aCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAgg                                 <  1:713309/93‑1 (MQ=255)

                                                        aCTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAgg                                 >  1:5325104/1‑93 (MQ=255)

                                                         cTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGc                                <  2:323922/93‑1 (MQ=255)

                                                          tAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGcc                               >  1:3327739/1‑93 (MQ=255)

                                                          tAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGcc                               >  2:1882777/1‑93 (MQ=255)

                                                              cTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCaaaa                           <  2:1586380/93‑1 (MQ=255)

                                                               tGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCaaaaa                          <  2:4506868/93‑1 (MQ=255)

                                                               tGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCaaaaa                          <  2:5131571/93‑1 (MQ=255)

                                                                 gCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAg                        <  1:4340261/93‑1 (MQ=255)

                                                                 gCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAg                        <  1:2983910/93‑1 (MQ=255)

                                                                  cGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGt                       <  1:2276621/93‑1 (MQ=255)

                                                                   gAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTc                      <  1:289166/93‑1 (MQ=255)

                                                                    aGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCt                     <  1:4632501/93‑1 (MQ=255)

                                                                    aGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCt                     >  1:3994589/1‑93 (MQ=255)

                                                                    aGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCt                     >  2:2119930/1‑93 (MQ=255)

                                                                       aGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTa                  >  1:3283569/1‑93 (MQ=255)

                                                                        aaaTAAAAATCAGCCCACCGATGGATAAGAGTGTTGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTaa                 <  2:3909789/92‑1 (MQ=255)

                                                                        aaaTAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTaa                 <  1:4091128/92‑1 (MQ=255)

                                                                          aTAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAatc               <  1:2363595/93‑1 (MQ=255)

                                                                             aaaaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAatcatc            >  1:834417/1‑93 (MQ=255)

                                                                             aaaaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAatcatc            <  2:2240396/93‑1 (MQ=255)

                                                                              aaaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATcc           <  1:1574591/93‑1 (MQ=255)

                                                                              aaaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATcc           <  2:5436050/93‑1 (MQ=255)

                                                                               aaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCa          <  2:3976230/93‑1 (MQ=255)

                                                                               aaTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCa          >  1:6297788/1‑93 (MQ=255)

                                                                                aTCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAg         >  2:1719817/1‑93 (MQ=255)

                                                                                    gCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAAc     >  1:4872260/1‑93 (MQ=255)

AATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGG  >  NC_000913/4518190‑4518369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCATC  >  NC_000913/4518187‑4518373

CTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACC                                                                                               <  SRR4302533.3552689/94‑1 (MQ=60)

CTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACC                                                                                               >  SRR4302533.2279925/1‑94 (MQ=60)

 TAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCG                                                                                              <  SRR4302533.1083243/94‑1 (MQ=60)

 TAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCG                                                                                              <  SRR4302533.4334540/94‑1 (MQ=60)

 TAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCG                                                                                              <  SRR4302533.529015/94‑1 (MQ=60)

     TTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGG                                                                                          <  SRR4302533.3224803/94‑1 (MQ=60)

      TATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGA                                                                                         <  SRR4302533.3236364/94‑1 (MQ=60)

        TGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATA                                                                                       >  SRR4302533.5165318/1‑94 (MQ=60)

         GGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAA                                                                                      <  SRR4302533.2998096/94‑1 (MQ=60)

          GGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAG                                                                                     >  SRR4302533.2286031/1‑94 (MQ=60)

          GGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAG                                                                                     <  SRR4302533.45744/94‑1 (MQ=60)

                AGTGAACACCAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTG                                                                               <  SRR4302533.1472761/94‑1 (MQ=60)

                AGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTG                                                                               <  SRR4302533.5841591/94‑1 (MQ=60)

                AGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTG                                                                               <  SRR4302533.4780931/94‑1 (MQ=60)

                    AACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACA                                                                           <  SRR4302533.4735463/94‑1 (MQ=60)

                     ACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACAT                                                                          <  SRR4302533.2402105/94‑1 (MQ=60)

                     ACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACAT                                                                          >  SRR4302533.1878983/1‑94 (MQ=60)

                     ACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACAT                                                                          >  SRR4302533.4287398/1‑94 (MQ=60)

                        ACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAG                                                                       <  SRR4302533.740184/94‑1 (MQ=60)

                        ACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAG                                                                       >  SRR4302533.1015113/1‑94 (MQ=60)

                          AGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCG                                                                     >  SRR4302533.5525239/1‑94 (MQ=60)

                          AGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCG                                                                     <  SRR4302533.2319428/94‑1 (MQ=60)

                             TTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCC                                                                  <  SRR4302533.3798748/94‑1 (MQ=60)

                             TTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCC                                                                  >  SRR4302533.3342600/1‑94 (MQ=60)

                              TGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATATAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCA                                                                 <  SRR4302533.3400171/94‑1 (MQ=60)

                              TGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCA                                                                 >  SRR4302533.5981613/1‑94 (MQ=60)

                               GGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAG                                                                <  SRR4302533.472559/94‑1 (MQ=60)

                               GGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAG                                                                <  SRR4302533.3860405/94‑1 (MQ=60)

                                GCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGG                                                               <  SRR4302533.4336014/94‑1 (MQ=60)

                                GCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGG                                                               <  SRR4302533.4540645/94‑1 (MQ=60)

                                 CTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGA                                                              >  SRR4302533.1532079/1‑94 (MQ=60)

                                 CTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGA                                                              <  SRR4302533.533841/94‑1 (MQ=60)

                                  TCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGAT                                                             >  SRR4302533.1677536/1‑94 (MQ=60)

                                  TCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGAT                                                             >  SRR4302533.1600870/1‑94 (MQ=60)

                                   CACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTTCAGGATA                                                            >  SRR4302533.5246208/1‑94 (MQ=60)

                                   CACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATA                                                            <  SRR4302533.3788179/94‑1 (MQ=60)

                                    ACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAA                                                           >  SRR4302533.2679342/1‑94 (MQ=60)

                                     CTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCGGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATACC                                                          >  SRR4302533.1427654/1‑94 (MQ=60)

                                     CTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAAC                                                          >  SRR4302533.1170291/1‑94 (MQ=60)

                                       GGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAG                                                        <  SRR4302533.3390401/94‑1 (MQ=60)

                                        GCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAcg                                                       >  SRR4302533.4013582/1‑92 (MQ=60)

                                         CAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCG                                                      <  SRR4302533.2731892/94‑1 (MQ=60)

                                         CAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCG                                                      <  SRR4302533.6015589/94‑1 (MQ=60)

                                           AGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTT                                                    <  SRR4302533.5812147/94‑1 (MQ=60)

                                           AGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTT                                                    <  SRR4302533.238727/94‑1 (MQ=60)

                                            GAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTT                                                   >  SRR4302533.3668069/1‑94 (MQ=60)

                                            GAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTT                                                   <  SRR4302533.5786742/94‑1 (MQ=60)

                                               CCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTT                                                >  SRR4302533.230960/1‑94 (MQ=60)

                                                CCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTT                                               <  SRR4302533.601088/94‑1 (MQ=60)

                                                CCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTT                                               <  SRR4302533.1892625/94‑1 (MQ=60)

                                                 CGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTG                                              <  SRR4302533.4329983/94‑1 (MQ=60)

                                                 CGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTG                                              <  SRR4302533.397994/94‑1 (MQ=60)

                                                 CGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTG                                              <  SRR4302533.5558214/94‑1 (MQ=60)

                                                 CGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTG                                              <  SRR4302533.906972/94‑1 (MQ=60)

                                                  GATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGG                                             >  SRR4302533.3380955/1‑94 (MQ=60)

                                                  GATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGG                                             >  SRR4302533.5740138/1‑94 (MQ=60)

                                                  GATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGG                                             <  SRR4302533.2112813/94‑1 (MQ=60)

                                                  GATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGG                                             <  SRR4302533.5900103/94‑1 (MQ=60)

                                                  GATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGG                                             <  SRR4302533.5500462/94‑1 (MQ=60)

                                                   ATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGT                                            <  SRR4302533.5108034/94‑1 (MQ=60)

                                                     TATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGG                                          >  SRR4302533.3071169/1‑94 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         >  SRR4302533.3520200/1‑94 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         <  SRR4302533.1919383/94‑1 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         >  SRR4302533.4938957/1‑94 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         >  SRR4302533.2240172/1‑94 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         <  SRR4302533.1816148/94‑1 (MQ=60)

                                                      ATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGT                                         >  SRR4302533.3191806/1‑94 (MQ=60)

                                                       TAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTC                                        <  SRR4302533.2107256/94‑1 (MQ=60)

                                                       TAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTC                                        <  SRR4302533.908126/94‑1 (MQ=60)

                                                       TAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTC                                        >  SRR4302533.4179276/1‑94 (MQ=60)

                                                        AAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA                                       <  SRR4302533.5135401/94‑1 (MQ=60)

                                                        AAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA                                       <  SRR4302533.2953849/94‑1 (MQ=60)

                                                        AAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA                                       >  SRR4302533.5485087/1‑94 (MQ=60)

                                                        AAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCA                                       <  SRR4302533.1085919/94‑1 (MQ=60)

                                                         AAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAG                                      <  SRR4302533.5853203/94‑1 (MQ=60)

                                                         AAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAG                                      >  SRR4302533.5964386/1‑94 (MQ=60)

                                                          AACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGG                                     <  SRR4302533.3119225/94‑1 (MQ=60)

                                                          AACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGG                                     >  SRR4302533.5357017/1‑94 (MQ=60)

                                                           ACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGC                                    <  SRR4302533.717517/94‑1 (MQ=60)

                                                            CTAAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCC                                   >  SRR4302533.3348303/1‑94 (MQ=60)

                                                              AAGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAA                                 >  SRR4302533.5459625/1‑94 (MQ=60)

                                                               AGCTGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAA                                >  SRR4302533.3121064/1‑94 (MQ=60)

                                                                  TGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAA                             >  SRR4302533.6023968/1‑94 (MQ=60)

                                                                  TGGCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAA                             <  SRR4302533.4509812/94‑1 (MQ=60)

                                                                    GCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGT                           <  SRR4302533.3002161/94‑1 (MQ=60)

                                                                    GCGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGT                           <  SRR4302533.4366422/94‑1 (MQ=60)

                                                                     CGAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTC                          <  SRR4302533.2290719/94‑1 (MQ=60)

                                                                      GAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCT                         <  SRR4302533.290930/94‑1 (MQ=60)

                                                                      GAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCT                         >  SRR4302533.1587128/1‑94 (MQ=60)

                                                                      GAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCT                         >  SRR4302533.4018910/1‑94 (MQ=60)

                                                                      GAGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCT                         <  SRR4302533.3755335/94‑1 (MQ=60)

                                                                       AGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTT                        <  SRR4302533.4660575/94‑1 (MQ=60)

                                                                       AGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTT                        <  SRR4302533.3696885/94‑1 (MQ=60)

                                                                       AGCAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTT                        <  SRR4302533.2776598/94‑1 (MQ=60)

                                                                         CAGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTA                      >  SRR4302533.3303839/1‑94 (MQ=60)

                                                                          AGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAA                     <  SRR4302533.458930/94‑1 (MQ=60)

                                                                          AGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAA                     <  SRR4302533.1917726/94‑1 (MQ=60)

                                                                          AGAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAA                     >  SRR4302533.1403904/1‑94 (MQ=60)

                                                                           AAATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAAT                    <  SRR4302533.4116032/94‑1 (MQ=60)

                                                                            AATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATC                   >  SRR4302533.5012256/1‑94 (MQ=60)

                                                                             ATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCA                  >  SRR4302533.2624605/1‑94 (MQ=60)

                                                                             ATAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCA                  <  SRR4302533.2378216/94‑1 (MQ=60)

                                                                              AAAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCAT                 <  SRR4302533.4902626/94‑1 (MQ=60)

                                                                               AAAAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATC                >  SRR4302533.839308/1‑94 (MQ=60)

                                                                                 AAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCA              >  SRR4302533.6335376/1‑94 (MQ=60)

                                                                                 AAATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCA              <  SRR4302533.1584075/94‑1 (MQ=60)

                                                                                   ATCAGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGA            <  SRR4302533.1682514/94‑1 (MQ=60)

                                                                                      AGCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAAC         >  SRR4302533.4901783/1‑94 (MQ=60)

                                                                                       GCCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACT        <  SRR4302533.5929347/94‑1 (MQ=60)

                                                                                        CCCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTG       >  SRR4302533.4049284/1‑94 (MQ=60)

                                                                                         CCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGG      >  SRR4302533.4695429/1‑94 (MQ=60)

                                                                                         CCACCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGG      >  SRR4302533.653273/1‑94 (MQ=60)

                                                                                            CCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCAT   <  SRR4302533.2483582/94‑1 (MQ=60)

                                                                                            CCGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCAT   >  SRR4302533.1535430/1‑94 (MQ=60)

                                                                                             CGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCATC  >  SRR4302533.2039376/1‑94 (MQ=60)

                                                                                             CGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCATC  <  SRR4302533.5660838/94‑1 (MQ=60)

                                                                                             CGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCATC  <  SRR4302533.1855253/94‑1 (MQ=60)

CTAAATTATGGGGGACAGTGAACAACAGGTTGGCTCACTGGCAAGAACCCGATTATAAAACTAAGCTGGCGAGCAGAATAAAAATCAGCCCACTGATGGATAAGAGTGTGGACATCAGCGTCCAGGATAACAGCGTTTCTTTGGTGGTCAGGCCAAAAAAGTCTTTAATCATCCAGAAACTGGCATC  >  NC_000913/4518187‑4518373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`