Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,600,078	C→T	W135* (TGG→TAG)	yjjP ←	putative structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,600,078	0	C	T	99.6%	1001.9 / NA	267	W135* (TGG→TAG)	yjjP	putative structural protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (152/114); minor base G (0/1); total (152/115)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599990‑4600167

    aagGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggg                                                                                   <  1:5765400/93‑1 (MQ=255)

     agatgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGt                                                                                  <  1:1044725/90‑1 (MQ=255)

     agGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTggga                                                                                  >  1:5185077/1‑92 (MQ=255)

      ggTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTa                                                                                 <  2:6048240/93‑1 (MQ=255)

        tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                               <  2:5725446/93‑1 (MQ=255)

        tgatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAAc                                                                               <  2:4054473/93‑1 (MQ=255)

         gatgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACg                                                                              >  1:1388950/1‑93 (MQ=255)

          atgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGt                                                                             <  2:439473/93‑1 (MQ=255)

           tgaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTa                                                                            >  1:2543587/1‑93 (MQ=255)

            gaCGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTaa                                                                           >  2:923425/1‑93 (MQ=255)

             aCGCCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAt                                                                          <  2:487135/93‑1 (MQ=255)

              cGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATg                                                                         <  2:3885552/93‑1 (MQ=255)

                  aCCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTg                                                                     >  2:5588745/1‑93 (MQ=255)

                   ccATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGa                                                                    >  1:787154/1‑93 (MQ=255)

                   ccATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGa                                                                    >  1:2180360/1‑93 (MQ=255)

                    cATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGaa                                                                   >  2:1253575/1‑93 (MQ=255)

                    cATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGaa                                                                   <  2:1842190/93‑1 (MQ=255)

                    cATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTAGGTAACGTAATGGCTGaa                                                                   <  2:1421171/93‑1 (MQ=255)

                     aTCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAt                                                                  <  1:2485509/93‑1 (MQ=255)

                     aTCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAt                                                                  <  1:5981889/93‑1 (MQ=255)

                      tCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 >  2:4546245/1‑93 (MQ=255)

                      ccccATCCACCGTTATTTAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAtt                                                                 <  2:1695318/92‑1 (MQ=255)

                       cccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAttt                                                                <  1:1535424/93‑1 (MQ=255)

                        ccAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTg                                                               >  1:1871315/1‑93 (MQ=255)

                          aGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGc                                                             >  2:393939/1‑93 (MQ=255)

                          aGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGc                                                             >  1:2134698/1‑93 (MQ=255)

                           gCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCt                                                            >  1:2603960/1‑93 (MQ=255)

                             cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                          >  1:2747792/1‑93 (MQ=255)

                             cACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaa                                                          >  2:3313479/1‑93 (MQ=255)

                              aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         <  2:2220893/93‑1 (MQ=255)

                              aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         >  2:3811700/1‑93 (MQ=255)

                              aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         <  1:5972841/93‑1 (MQ=255)

                              aCCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTaaa                                                         <  2:5523774/93‑1 (MQ=255)

                               ccGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAt                                                        >  2:5965968/1‑93 (MQ=255)

                                cGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATc                                                       <  2:4169415/93‑1 (MQ=255)

                                 gTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCg                                                      >  2:5783034/1‑93 (MQ=255)

                                  ttATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGt                                                     <  1:4768424/93‑1 (MQ=255)

                                   tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                    <  1:1768793/93‑1 (MQ=255)

                                   tATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGtt                                                    >  2:1786697/1‑93 (MQ=255)

                                    aTTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGttt                                                   >  1:143088/1‑93 (MQ=255)

                                    aTTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGttt                                                   >  1:4228025/1‑93 (MQ=255)

                                     ttGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtc                                                  >  2:3085176/1‑93 (MQ=255)

                                     ttGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtc                                                  <  1:3709992/93‑1 (MQ=255)

                                      tGAGTTTACAGAAACAGGCGCAAGAAAGGCAAACCATTAAGGCTACCAGCGATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTtct                                                 <  2:1162235/93‑1 (MQ=255)

                                         gTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTa                                              <  1:3044189/93‑1 (MQ=255)

                                          tttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAc                                             <  2:5268870/93‑1 (MQ=255)

                                          tttACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAc                                             >  2:5401710/1‑93 (MQ=255)

                                              cAGAAACAAGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCt                                         >  2:6155310/1‑93 (MQ=255)

                                                gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       >  2:2961459/1‑93 (MQ=255)

                                                gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       >  2:4842051/1‑93 (MQ=255)

                                                gAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCttt                                       >  1:4882303/1‑93 (MQ=255)

                                                 aaaCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTg                                      <  1:1243373/93‑1 (MQ=255)

                                                   aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTa                                    >  1:5291691/1‑93 (MQ=255)

                                                   aCAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTa                                    >  1:2928173/1‑93 (MQ=255)

                                                     aGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAAt                                  <  2:5670506/93‑1 (MQ=255)

                                                      ggCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATc                                 >  1:3450265/1‑93 (MQ=255)

                                                      ggCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATc                                 >  2:1427547/1‑93 (MQ=255)

                                                       gcgcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATcc                                <  1:4444942/93‑1 (MQ=255)

                                                        cgcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCca                               <  1:5368646/93‑1 (MQ=255)

                                                         gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcag                              <  1:6227307/93‑1 (MQ=255)

                                                         gcAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcac                              >  2:4489906/1‑92 (MQ=255)

                                                          cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagc                             >  1:5133555/1‑93 (MQ=255)

                                                          cAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagc                             <  1:5527721/93‑1 (MQ=255)

                                                           aaGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagca                            >  1:283987/1‑93 (MQ=255)

                                                            aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           >  1:655526/1‑93 (MQ=255)

                                                            aGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCcagcag                           >  1:5911940/1‑93 (MQ=255)

                                                             gAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAga                          <  1:4997428/93‑1 (MQ=255)

                                                              aaaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgat                         <  1:2959600/93‑1 (MQ=255)

                                                              aaaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgat                         <  1:5252903/93‑1 (MQ=255)

                                                              aaaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgat                         >  1:5596271/1‑93 (MQ=255)

                                                               aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatg                        <  2:2080014/93‑1 (MQ=255)

                                                               aaGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAgatg                        >  1:3231712/1‑93 (MQ=255)

                                                                     aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                  <  2:2459742/93‑1 (MQ=255)

                                                                     aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                  >  1:2371280/1‑93 (MQ=255)

                                                                     aaCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTc                  >  2:2549289/1‑93 (MQ=255)

                                                                      aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                 >  2:1945424/1‑93 (MQ=255)

                                                                      aCCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTcc                 <  2:1645373/93‑1 (MQ=255)

                                                                        cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               >  1:2768912/1‑93 (MQ=255)

                                                                        cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               >  2:1545281/1‑93 (MQ=255)

                                                                        cATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGc               >  1:6168922/1‑93 (MQ=255)

                                                                         aTTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa              >  2:1186073/1‑93 (MQ=255)

                                                                         aTTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCa              >  2:5095941/1‑93 (MQ=255)

                                                                          ttAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCAACGCCTTTGTAATCCAGCAGATGATGCTCCGCaa             <  2:2794056/93‑1 (MQ=255)

                                                                           tAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAg            >  2:1046171/1‑93 (MQ=255)

                                                                           tAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAg            >  2:5999016/1‑93 (MQ=255)

                                                                           tAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAg            <  2:4991853/93‑1 (MQ=255)

                                                                            aaGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGa           <  1:729549/93‑1 (MQ=255)

                                                                              ggCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAt         >  2:3491427/1‑93 (MQ=255)

                                                                              ggCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAt         >  1:4876388/1‑93 (MQ=255)

                                                                                cTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATca       <  2:3700057/93‑1 (MQ=255)

                                                                                 tACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcac      >  2:237314/1‑93 (MQ=255)

                                                                                  aCCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATcaca     >  1:3961865/1‑93 (MQ=255)

                                                                                   ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    >  1:158802/1‑93 (MQ=255)

                                                                                   ccAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACaa    >  1:1719572/1‑93 (MQ=255)

                                                                                    cAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAt   >  1:4530407/1‑93 (MQ=255)

                                                                                     aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  >  1:401667/1‑93 (MQ=255)

                                                                                     aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  <  1:3994220/93‑1 (MQ=255)

                                                                                     aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  <  1:6105989/93‑1 (MQ=255)

                                                                                     aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  >  2:2190231/1‑93 (MQ=255)

                                                                                     aGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAtg  >  1:882448/1‑93 (MQ=255)

AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG  >  NC_000913/4599990‑4600167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  >  NC_000913/4599985‑4600171

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCT                                                                                               >  SRR4302533.1633217/1‑94 (MQ=60)

     AAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTT                                                                                          >  SRR4302533.5593073/1‑94 (MQ=60)

      AAGAAGGTGACGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTG                                                                                         <  SRR4302533.5956389/94‑1 (MQ=60)

       AGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGG                                                                                        <  SRR4302533.5164665/94‑1 (MQ=60)

       AGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGG                                                                                        <  SRR4302533.4152589/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGT                                                                                      <  SRR4302533.5800191/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGT                                                                                      <  SRR4302533.427519/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGT                                                                                      <  SRR4302533.3176004/94‑1 (MQ=60)

         AAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGA                                                                                      >  SRR4302533.5216376/1‑94 (MQ=60)

          AGATGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTA                                                                                     <  SRR4302533.1050866/94‑1 (MQ=60)

             TGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACG                                                                                  >  SRR4302533.1397316/1‑94 (MQ=60)

               ATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTA                                                                                >  SRR4302533.2559238/1‑94 (MQ=60)

                TGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAA                                                                               <  SRR4302533.5468213/94‑1 (MQ=60)

                TGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAA                                                                               <  SRR4302533.2419392/94‑1 (MQ=60)

                 GACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAAT                                                                              <  SRR4302533.1591031/94‑1 (MQ=60)

                  ACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATG                                                                             <  SRR4302533.1620389/94‑1 (MQ=60)

                    GGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGC                                                                           <  SRR4302533.2889592/94‑1 (MQ=60)

                     GCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCT                                                                          >  SRR4302533.3657965/1‑94 (MQ=60)

                     GCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCT                                                                          >  SRR4302533.717681/1‑94 (MQ=60)

                      CACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTG                                                                         <  SRR4302533.630316/94‑1 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        >  SRR4302533.791779/1‑94 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        >  SRR4302533.2193803/1‑94 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        <  SRR4302533.5945070/94‑1 (MQ=60)

                       ACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGA                                                                        >  SRR4302533.5350378/1‑94 (MQ=60)

                        CCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAA                                                                       <  SRR4302533.1673433/94‑1 (MQ=60)

                         CATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAT                                                                      >  SRR4302533.3689555/1‑94 (MQ=60)

                         CATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAAT                                                                      >  SRR4302533.2725973/1‑94 (MQ=60)

                         CATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGCCTACCAGCTATCTTGGGTAACGTAATGGCTGAAT                                                                      <  SRR4302533.6178812/94‑1 (MQ=60)

                          ATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     <  SRR4302533.2873838/94‑1 (MQ=60)

                          ATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     <  SRR4302533.2500824/94‑1 (MQ=60)

                          ATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATT                                                                     <  SRR4302533.6018120/94‑1 (MQ=60)

                            CCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTG                                                                   >  SRR4302533.1882723/1‑94 (MQ=60)

                            CCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTG                                                                   <  SRR4302533.1544650/94‑1 (MQ=60)

                            CCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCCTGGGTAACGTAATGGCTGAATTTG                                                                   >  SRR4302533.3220266/1‑94 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  >  SRR4302533.6263405/1‑94 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  >  SRR4302533.1751478/1‑94 (MQ=60)

                             CCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGG                                                                  <  SRR4302533.733732/94‑1 (MQ=60)

                              CAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGC                                                                 <  SRR4302533.3061988/94‑1 (MQ=60)

                              CAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGC                                                                 >  SRR4302533.2147861/1‑94 (MQ=60)

                               AGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCT                                                                >  SRR4302533.2564017/1‑94 (MQ=60)

                               AGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCT                                                                >  SRR4302533.2619956/1‑94 (MQ=60)

                               AGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCT                                                                >  SRR4302533.5409807/1‑94 (MQ=60)

                                GCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTA                                                               >  SRR4302533.1411744/1‑94 (MQ=60)

                                 CCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAA                                                              >  SRR4302533.2764637/1‑94 (MQ=60)

                                 CCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAA                                                              >  SRR4302533.3755006/1‑94 (MQ=60)

                                  CACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAA                                                             <  SRR4302533.278652/94‑1 (MQ=60)

                                   ACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAT                                                            >  SRR4302533.1399178/1‑94 (MQ=60)

                                   ACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAAT                                                            <  SRR4302533.6008994/94‑1 (MQ=60)

                                    CCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATC                                                           >  SRR4302533.4538712/1‑94 (MQ=60)

                                      GTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGT                                                         <  SRR4302533.6032429/94‑1 (MQ=60)

                                      GTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGT                                                         >  SRR4302533.5586070/1‑94 (MQ=60)

                                      GTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGT                                                         <  SRR4302533.3317615/94‑1 (MQ=60)

                                      GTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGT                                                         <  SRR4302533.2793570/94‑1 (MQ=60)

                                       TTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTT                                                        <  SRR4302533.4797328/94‑1 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       >  SRR4302533.4253587/1‑94 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       >  SRR4302533.788968/1‑94 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       >  SRR4302533.144001/1‑94 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       >  SRR4302533.2967679/1‑94 (MQ=60)

                                        TATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTT                                                       <  SRR4302533.1779563/94‑1 (MQ=60)

                                         ATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTC                                                      >  SRR4302533.4880189/1‑94 (MQ=60)

                                          TTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCT                                                     <  SRR4302533.6015798/94‑1 (MQ=60)

                                          TTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCT                                                     <  SRR4302533.5828542/94‑1 (MQ=60)

                                          TTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCT                                                     <  SRR4302533.3732777/94‑1 (MQ=60)

                                          ATGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCT                                                     <  SRR4302533.5016746/94‑1 (MQ=60)

                                           TGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTC                                                    <  SRR4302533.4151280/94‑1 (MQ=60)

                                            GAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCT                                                   >  SRR4302533.6292885/1‑94 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 >  SRR4302533.1802646/1‑94 (MQ=60)

                                              GTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTAC                                                 <  SRR4302533.3062823/94‑1 (MQ=60)

                                                TTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGC                                               >  SRR4302533.4921320/1‑94 (MQ=60)

                                                   CAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTT                                            >  SRR4302533.3039561/1‑94 (MQ=60)

                                                    AGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTT                                           >  SRR4302533.4911880/1‑94 (MQ=60)

                                                      AAACAGGCGCAAGACAAGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTACTGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGT                                         <  SRR4302533.2191395/94‑1 (MQ=60)

                                                      AAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGT                                         <  SRR4302533.1250798/94‑1 (MQ=60)

                                                       AACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTA                                        >  SRR4302533.5323373/1‑94 (MQ=60)

                                                       AACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTA                                        >  SRR4302533.2946086/1‑94 (MQ=60)

                                                        ACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAA                                       >  SRR4302533.2571892/1‑94 (MQ=60)

                                                          AGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATC                                     >  SRR4302533.4445329/1‑94 (MQ=60)

                                                          AGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATC                                     >  SRR4302533.2776142/1‑94 (MQ=60)

                                                          AGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATC                                     >  SRR4302533.3471569/1‑94 (MQ=60)

                                                          AGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATC                                     >  SRR4302533.874067/1‑94 (MQ=60)

                                                           GGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCC                                    <  SRR4302533.5290824/94‑1 (MQ=60)

                                                            GCGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCA                                   <  SRR4302533.4471826/94‑1 (MQ=60)

                                                             CGCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAG                                  <  SRR4302533.5400896/94‑1 (MQ=60)

                                                              GCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGC                                 >  SRR4302533.5164568/1‑94 (MQ=60)

                                                              GCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGC                                 >  SRR4302533.5090329/1‑94 (MQ=60)

                                                              GCAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGC                                 <  SRR4302533.6264615/94‑1 (MQ=60)

                                                               CAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCA                                <  SRR4302533.5561091/94‑1 (MQ=60)

                                                               CAAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCA                                >  SRR4302533.285724/1‑94 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               <  SRR4302533.5105713/94‑1 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               >  SRR4302533.5947664/1‑94 (MQ=60)

                                                                AAGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAG                               >  SRR4302533.659395/1‑94 (MQ=60)

                                                                 AGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGA                              >  SRR4302533.1576466/1‑94 (MQ=60)

                                                                 AGAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGA                              >  SRR4302533.4869096/1‑94 (MQ=60)

                                                                  GAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGAT                             >  SRR4302533.1274233/1‑94 (MQ=60)

                                                                  GAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGAT                             <  SRR4302533.5027726/94‑1 (MQ=60)

                                                                  GAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGAT                             >  SRR4302533.5630084/1‑94 (MQ=60)

                                                                  GAAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGAT                             >  SRR4302533.1191667/1‑94 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            <  SRR4302533.473880/94‑1 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            <  SRR4302533.2977690/94‑1 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            >  SRR4302533.3251630/1‑94 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            <  SRR4302533.5284464/94‑1 (MQ=60)

                                                                   AAAGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATG                            >  SRR4302533.3997104/1‑94 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302533.5693109/94‑1 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          >  SRR4302533.772477/1‑94 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302533.2243236/94‑1 (MQ=60)

                                                                     AGGCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGAT                          <  SRR4302533.4740442/94‑1 (MQ=60)

                                                                       GCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGC                        <  SRR4302533.4006656/94‑1 (MQ=60)

                                                                       GCCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGC                        >  SRR4302533.2076915/1‑94 (MQ=60)

                                                                        CCAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCT                       >  SRR4302533.1793737/1‑94 (MQ=60)

                                                                         CAACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTC                      >  SRR4302533.2385950/1‑94 (MQ=60)

                                                                           ACCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCG                    <  SRR4302533.6141007/94‑1 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302533.6206005/1‑94 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302533.2785904/1‑94 (MQ=60)

                                                                            CCATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGC                   >  SRR4302533.5242093/1‑94 (MQ=60)

                                                                             CATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCA                  >  SRR4302533.930951/1‑94 (MQ=60)

                                                                              ATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAA                 >  SRR4302533.1537140/1‑94 (MQ=60)

                                                                              ATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAA                 >  SRR4302533.2643264/1‑94 (MQ=60)

                                                                              ATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAA                 >  SRR4302533.6146990/1‑94 (MQ=60)

                                                                              ATTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAA                 >  SRR4302533.4025816/1‑94 (MQ=60)

                                                                               TTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAG                <  SRR4302533.5303310/94‑1 (MQ=60)

                                                                               TTAAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAG                <  SRR4302533.3963696/94‑1 (MQ=60)

                                                                                 AAGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAA              <  SRR4302533.733852/94‑1 (MQ=60)

                                                                                  AGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAT             >  SRR4302533.4905931/1‑94 (MQ=60)

                                                                                  AGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAT             <  SRR4302533.1261070/94‑1 (MQ=60)

                                                                                  AGGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAAT             >  SRR4302533.4905932/1‑94 (MQ=60)

                                                                                   GGCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATC            >  SRR4302533.915150/1‑94 (MQ=60)

                                                                                    GCTACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCA           <  SRR4302533.2522116/94‑1 (MQ=60)

                                                                                      TACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACA         >  SRR4302533.5266605/1‑94 (MQ=60)

                                                                                      TACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACA         >  SRR4302533.3986003/1‑94 (MQ=60)

                                                                                       ACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAA        >  SRR4302533.159814/1‑94 (MQ=60)

                                                                                       ACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAA        >  SRR4302533.1729997/1‑94 (MQ=60)

                                                                                       ACCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAA        <  SRR4302533.5980506/94‑1 (MQ=60)

                                                                                        CCAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAAT       >  SRR4302533.4557841/1‑94 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      >  SRR4302533.887631/1‑94 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      >  SRR4302533.404090/1‑94 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      <  SRR4302533.2325280/94‑1 (MQ=60)

                                                                                         CAGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATG      >  SRR4302533.2094524/1‑94 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     <  SRR4302533.2456684/94‑1 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     <  SRR4302533.4018540/94‑1 (MQ=60)

                                                                                          AGCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGT     <  SRR4302533.6142859/94‑1 (MQ=60)

                                                                                           GCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTG    <  SRR4302533.4910050/94‑1 (MQ=60)

                                                                                           GCTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTG    >  SRR4302533.117427/1‑94 (MQ=60)

                                                                                            CTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGC   <  SRR4302533.5675818/94‑1 (MQ=60)

                                                                                            CTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGC   >  SRR4302533.1368764/1‑94 (MQ=60)

                                                                                            CTATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGC   >  SRR4302533.4556702/1‑94 (MQ=60)

                                                                                             TATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  >  SRR4302533.769413/1‑94 (MQ=60)

                                                                                             TATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  <  SRR4302533.6100472/94‑1 (MQ=60)

CTGGCAAAGAAGGTGATGACGGCACCATCCCAGCCACCGTTATTGAGTTTACAGAAACAGGCGCAAGAAAGGCCAACCATTAAGGCTACCAGCCATCTTGGGTAACGTAATGGCTGAATTTGGCTAAATCGTTTCTCTACGCCTTTGTAATCCAGCAGATGATGCTCCGCAAGAATCACAATGTGCT  >  NC_000913/4599985‑4600171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`