Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,256,893 +C coding (405/423 nt) yhaQ ← putative L‑serine dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,8911.C99.6% 1847.0 / ‑2.2 530coding (407/423 nt)yhaQputative L‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (265/263);  total (266/264)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 578 total aligned reads displayed.

CGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC  >  NC_000913/3256798‑3256979
                                                                                              |                                                                                        
 gTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                         <  2:730510/94‑1 (MQ=255)
  tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cc                                                                                        <  1:1697778/94‑1 (MQ=255)
   ttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                       >  1:10581472/1‑93 (MQ=255)
    tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccct                                                                                      <  2:4877745/94‑3 (MQ=255)
     cGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAGCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                     <  2:8912801/94‑4 (MQ=255)
     cGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                     <  1:9912337/94‑4 (MQ=255)
        cATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCa                                                                                   >  2:4668936/1‑94 (MQ=255)
           cGTGCGACATGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGCGTAATAGGTTTTACTCAATAGCTCTTCCTGTCCCTTCCAgcc                                                                                >  2:9830255/1‑92 (MQ=255)
             tGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAg                                                                              >  2:9117289/1‑94 (MQ=255)
               cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                            >  1:10051985/1‑94 (MQ=255)
               cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTg                                                                            >  1:756865/1‑94 (MQ=255)
                gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                           >  1:10212525/1‑94 (MQ=255)
                gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                           >  2:868163/1‑94 (MQ=255)
                gACAGGCCGAAGCGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGa                                                                           >  2:5179790/1‑94 (MQ=255)
                  cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATc                                                                         >  2:5457561/1‑94 (MQ=255)
                  cAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATc                                                                         >  2:8234704/1‑94 (MQ=255)
                   aGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATcc                                                                        <  1:4472816/94‑1 (MQ=255)
                     gCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGc                                                                      >  2:6149430/1‑94 (MQ=255)
                        gAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCAtt                                                                   >  2:1943944/1‑94 (MQ=255)
                          aGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTcc                                                                 <  2:5865741/94‑1 (MQ=255)
                           gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCg                                                                <  2:7898713/94‑1 (MQ=255)
                            tgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGt                                                               <  2:8117395/94‑1 (MQ=255)
                             gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGtt                                                              >  2:2368991/1‑94 (MQ=255)
                               tcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtct                                                            >  1:8535018/1‑94 (MQ=255)
                                cttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTtctc                                                           <  2:432727/94‑1 (MQ=255)
                                 ttcttgGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                          <  2:7568989/94‑1 (MQ=255)
                                  tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTCTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGCGATCCGCATTCCGTTCTCAt                                                         >  1:3633464/1‑94 (MQ=255)
                                   cttcGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATg                                                        <  1:5042294/94‑1 (MQ=255)
                                      cGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCg                                                     <  2:2682967/94‑1 (MQ=255)
                                         cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAgg                                                  <  1:2559529/94‑1 (MQ=255)
                                            tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa                                               >  1:8176100/1‑94 (MQ=255)
                                               aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAt                                            <  1:3270220/94‑1 (MQ=255)
                                                cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                           >  1:6772331/1‑94 (MQ=255)
                                                cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                           >  1:7413255/1‑94 (MQ=255)
                                                cccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGAGAGGCGACaat                                           >  1:5923798/1‑92 (MQ=255)
                                                 ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                          <  2:3703102/94‑1 (MQ=255)
                                                 ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                          <  2:1612609/94‑1 (MQ=255)
                                                 ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                          <  1:1299087/94‑1 (MQ=255)
                                                  cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                         >  2:3928855/1‑94 (MQ=255)
                                                  cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                         <  2:3529246/94‑1 (MQ=255)
                                                  cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                         >  1:7189862/1‑94 (MQ=255)
                                                  cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTc                                         <  1:2958634/94‑1 (MQ=255)
                                                   gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                        <  1:7725599/94‑1 (MQ=255)
                                                   gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                        >  1:2690029/1‑94 (MQ=255)
                                                   gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                        <  2:6751162/94‑1 (MQ=255)
                                                   gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                        <  1:8778595/94‑1 (MQ=255)
                                                   gccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCg                                        <  1:6501230/94‑1 (MQ=255)
                                                    ccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                       <  2:5094165/94‑1 (MQ=255)
                                                       ccgACAGAGTATTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAt                                    <  1:6105275/94‑1 (MQ=255)
                                                        cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                   >  1:9460873/1‑94 (MQ=255)
                                                        cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                   <  2:1033883/94‑1 (MQ=255)
                                                        cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                   >  2:33013/1‑94 (MQ=255)
                                                        cgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGACAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                   >  1:6282366/1‑94 (MQ=255)
                                                         gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                  <  1:3727937/94‑1 (MQ=255)
                                                         gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGCGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                  >  1:9627992/1‑94 (MQ=255)
                                                         caCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                  <  1:7260345/93‑1 (MQ=255)
                                                          aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                 <  1:7015018/94‑1 (MQ=255)
                                                           cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                                <  1:2810691/94‑1 (MQ=255)
                                                            agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATTGCGAGGCAACATTTCGGGATGGAAg                               <  1:5436051/94‑1 (MQ=255)
                                                            agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                               >  1:4274608/1‑94 (MQ=255)
                                                            agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                               >  2:6940950/1‑94 (MQ=255)
                                                            agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAg                               <  2:653195/94‑1 (MQ=255)
                                                              agTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                             <  2:1045846/94‑1 (MQ=255)
                                                               gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATa                            >  1:10342272/1‑94 (MQ=255)
                                                                 aaTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt                          >  2:2887697/1‑94 (MQ=255)
                                                                  aTAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                         >  2:215957/1‑94 (MQ=255)
                                                                  aTAGGTGTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATGCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                         <  1:4633224/94‑1 (MQ=255)
                                                                   tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                        <  1:3135530/94‑1 (MQ=255)
                                                                   tAGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                        >  1:2110141/1‑94 (MQ=255)
                                                                    aGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTGGGGGTGAAAGATAATGtt                       >  2:3129886/1‑94 (MQ=255)
                                                                    aGGTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGtt                       <  2:6276274/94‑1 (MQ=255)
                                                                     ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                      >  2:1340563/1‑94 (MQ=255)
                                                                     ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                      <  1:9846948/94‑1 (MQ=255)
                                                                     ggTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                      >  1:6911915/1‑94 (MQ=255)
                                                                      gTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                     <  1:4861772/94‑1 (MQ=255)
                                                                      gTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                     >  1:911916/1‑94 (MQ=255)
                                                                      gTTTTACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                     >  2:5590950/1‑94 (MQ=255)
                                                                        tttACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                   >  1:1136686/1‑94 (MQ=255)
                                                                          tACTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                 >  2:2585712/1‑94 (MQ=255)
                                                                            cTTAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTa               >  1:5787269/1‑94 (MQ=255)
                                                                             ttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc              >  1:1896941/1‑94 (MQ=255)
                                                                             ttAATAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc              >  1:2280623/1‑94 (MQ=255)
                                                                               aaTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAg            >  1:2427153/1‑94 (MQ=255)
                                                                                aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAgg           >  1:8381335/1‑94 (MQ=255)
                                                                                aTAGCTCTTCCTGTCCCTTCCAGGCAGTGATACGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAgg           >  2:4056370/1‑94 (MQ=255)
                                                                                  aGCTCTACCAGTCCCTTCCAGGCAGTGATCAGCATTCCGTTATAATGGCGGGACAACATATCAGGATAGACGATAATGTTCTTTGCTACAGGat         <  2:9359337/94‑2 (MQ=255)
                                                                                   gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa        <  2:776380/94‑1 (MQ=255)
                                                                                   gCTCTTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGCTCTTTGCTACAGGaaa        >  2:8497864/1‑94 (MQ=255)
                                                                                    ctctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa       <  2:9752682/94‑1 (MQ=255)
                                                                                    ctctTCCTGTACCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa       <  2:5269416/94‑1 (MQ=255)
                                                                                     tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt      >  1:519631/1‑94 (MQ=255)
                                                                                     tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt      <  1:7927152/94‑1 (MQ=255)
                                                                                     tctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt      <  1:2011589/94‑1 (MQ=255)
                                                                                      ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc     <  1:4019239/94‑1 (MQ=255)
                                                                                      ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc     <  1:3507441/94‑1 (MQ=255)
                                                                                      ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc     >  2:7144839/1‑94 (MQ=255)
                                                                                      ctTCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc     <  2:4491132/94‑1 (MQ=255)
                                                                                       ttCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATca    <  1:10419889/94‑1 (MQ=255)
                                                                                        tCCTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa   >  1:9548839/1‑94 (MQ=255)
                                                                                         ccTGTCCCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaac  <  2:10144674/94‑1 (MQ=255)
                                                                                              |                                                                                        
CGTTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC  >  NC_000913/3256798‑3256979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 289 total aligned reads displayed.
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257745‑3257931
                                                                                               |                                                                                             
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑G                                                                                               <  SRR4302545.7025932/94‑1 (MQ=60)
  TTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑Ggc                                                                                             <  SRR4302545.2444606/94‑3 (MQ=60)
    TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGT                                                                                            <  SRR4302545.3332525/94‑1 (MQ=60)
    TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGT                                                                                            <  SRR4302545.3650621/94‑1 (MQ=60)
    TCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGT                                                                                            <  SRR4302545.2860403/94‑1 (MQ=60)
     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTA                                                                                           >  SRR4302545.5682263/1‑94 (MQ=60)
     CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTA                                                                                           <  SRR4302545.6875856/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAG                                                                                          >  SRR4302545.1015707/1‑94 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAG                                                                                          <  SRR4302545.10809814/94‑1 (MQ=60)
      ATACATCCTCCGGCGTCAGAGCCCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAG                                                                                          <  SRR4302545.4284637/94‑1 (MQ=60)
       TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGC                                                                                         >  SRR4302545.9133210/1‑94 (MQ=60)
       TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGC                                                                                         >  SRR4302545.365509/1‑94 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCC                                                                                        <  SRR4302545.6437088/94‑1 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCC                                                                                        <  SRR4302545.830391/94‑1 (MQ=60)
        ACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCC                                                                                        >  SRR4302545.361881/1‑94 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCA                                                                                       >  SRR4302545.714693/1‑94 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCA                                                                                       >  SRR4302545.59994/1‑94 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCA                                                                                       >  SRR4302545.3367627/1‑94 (MQ=60)
         CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCA                                                                                       <  SRR4302545.9956186/94‑1 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAG                                                                                      >  SRR4302545.3137345/1‑94 (MQ=60)
          ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAG                                                                                      <  SRR4302545.1316066/94‑1 (MQ=60)
           TCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGA                                                                                     <  SRR4302545.3130844/94‑1 (MQ=60)
           TCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGA                                                                                     >  SRR4302545.5470624/1‑94 (MQ=60)
            CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAG                                                                                    >  SRR4302545.9844932/1‑94 (MQ=60)
            CCTCCGGCGTCAGAGCGCCTGAGTAAAGGGACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAG                                                                                    >  SRR4302545.5388466/1‑94 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGA                                                                                   >  SRR4302545.3334789/1‑94 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGA                                                                                   >  SRR4302545.4220148/1‑94 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGA                                                                                   >  SRR4302545.5760188/1‑94 (MQ=60)
             CTCCGGCGTCAGAGCGCCTGGGAAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑Ggcgtagacagagc                                                                                  >  SRR4302545.3482734/1‑81 (MQ=60)
              TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGAC                                                                                  <  SRR4302545.7331403/94‑1 (MQ=60)
              TCCGCCGTAAGAGCTGCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGAC                                                                                  <  SRR4302545.8024920/94‑1 (MQ=60)
               CCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACA                                                                                 <  SRR4302545.1885663/94‑1 (MQ=60)
                CGGCGTCAGAGCGCCTGGGTAAAGGTATGTGAAATAACATCCTGTTGCTGTTCACGGGTCTGTGCGTCGAAGCGCA‑CGGCGTAGCCAGAGACAC                                                                                >  SRR4302545.9665953/1‑94 (MQ=60)
                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACAC                                                                                >  SRR4302545.6271094/1‑94 (MQ=60)
                CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACAC                                                                                >  SRR4302545.9920346/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAATCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.6030973/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.10551046/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.223892/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.5088379/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.3071104/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               <  SRR4302545.907795/94‑1 (MQ=60)
                 GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACG                                                                               >  SRR4302545.4620486/1‑94 (MQ=60)
                 GGCGTCAGAGCGCCTAGGAAAAGGTACGTGATATAACATCCTGTTGCTGTTCACGGATCAGAGAGTTGAAGCGCA‑C‑Ggcgcagtcacagacacg                                                                              >  SRR4302545.5633257/1‑77 (MQ=58)
                  GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGG                                                                              <  SRR4302545.10462325/94‑1 (MQ=60)
                  GCGTCAGAGCGCCTGGGTAAAGGCACGTGAAATAACATCCTGTGGCTGTTCCCGCGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGG                                                                              <  SRR4302545.5922392/94‑1 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGA                                                                             <  SRR4302545.2222758/94‑1 (MQ=60)
                   CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGA                                                                             >  SRR4302545.8363097/1‑94 (MQ=60)
                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTG                                                                          <  SRR4302545.3169790/94‑1 (MQ=60)
                      CAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTG                                                                          >  SRR4302545.4037847/1‑94 (MQ=60)
                       AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGT                                                                         >  SRR4302545.7605967/1‑94 (MQ=60)
                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCA                                                                       <  SRR4302545.10930903/94‑1 (MQ=60)
                         AGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCA                                                                       <  SRR4302545.5075324/94‑1 (MQ=60)
                           CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGG                                                                     <  SRR4302545.5525166/94‑1 (MQ=60)
                            GCCTGGGTATATGTACGTGAAATATCATCCTGTTCTTGTTCACGTGGCAGTTCGTTGAAGCGCT‑CGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    <  SRR4302545.4696213/94‑1 (MQ=36)
                            GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    <  SRR4302545.7316993/94‑1 (MQ=60)
                            GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    <  SRR4302545.2256411/94‑1 (MQ=60)
                            GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    >  SRR4302545.7499715/1‑94 (MQ=60)
                            GCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    >  SRR4302545.5032196/1‑94 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                   <  SRR4302545.6516437/94‑1 (MQ=60)
                             CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                   <  SRR4302545.8650466/94‑1 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGCTTGTCAagttt                                                                  >  SRR4302545.3919545/1‑89 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  >  SRR4302545.6186799/1‑94 (MQ=60)
                              CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  >  SRR4302545.7963420/1‑94 (MQ=60)
                               gtggtaacgtTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 <  SRR4302545.8499536/84‑1 (MQ=60)
                               TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 <  SRR4302545.2545852/94‑1 (MQ=60)
                               TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 <  SRR4302545.9384820/94‑1 (MQ=60)
                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                                >  SRR4302545.4935369/1‑94 (MQ=60)
                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                                <  SRR4302545.936910/94‑1 (MQ=60)
                                GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                                >  SRR4302545.1362994/1‑94 (MQ=60)
                                 GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               >  SRR4302545.10072748/1‑94 (MQ=60)
                                 GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               >  SRR4302545.3740966/1‑94 (MQ=60)
                                 GGTAAAGGTACGTAAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               <  SRR4302545.1420806/94‑1 (MQ=60)
                                  GTAAAGGTATGTTAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                              <  SRR4302545.9056516/94‑1 (MQ=60)
                                  GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                              <  SRR4302545.7823063/94‑1 (MQ=60)
                                   TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             >  SRR4302545.6542273/1‑94 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            <  SRR4302545.10037610/94‑1 (MQ=60)
                                    AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            >  SRR4302545.8358462/1‑94 (MQ=60)
                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                           <  SRR4302545.781516/94‑1 (MQ=60)
                                     AAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATT                                                           <  SRR4302545.377552/94‑1 (MQ=60)
                                        GTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTT                                                        <  SRR4302545.5461086/94‑1 (MQ=60)
                                         TACGTGAAATAACATCCGGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                       <  SRR4302545.5643117/94‑1 (MQ=60)
                                          acgaaaaaaAACATCCTGTTGCTGTTCACGGTTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                      <  SRR4302545.10128115/85‑1 (MQ=60)
                                          ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                      >  SRR4302545.2059968/1‑94 (MQ=60)
                                          ACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCC                                                      <  SRR4302545.2660740/94‑1 (MQ=60)
                                           CGTGAAATAACATTCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACTCGGATTGTCAGGCTAGGATATTTTTACG                                                     >  SRR4302545.5567214/1‑94 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     >  SRR4302545.5056825/1‑94 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     >  SRR4302545.2993380/1‑94 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     >  SRR4302545.9940147/1‑94 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302545.10594606/94‑1 (MQ=60)
                                           CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302545.6357739/94‑1 (MQ=60)
                                           CGGGAAATAACATCCTGTTGCTGTTCACGGGTCAGGGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATCGTCAGGATAGGATATTTTTTCG                                                     >  SRR4302545.5993532/1‑94 (MQ=60)
                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                    >  SRR4302545.2431163/1‑94 (MQ=60)
                                            GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGCTAGGATATTTTTCCGG                                                    >  SRR4302545.1284408/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   >  SRR4302545.9767178/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   <  SRR4302545.8421191/94‑1 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   >  SRR4302545.10063977/1‑94 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   <  SRR4302545.4216491/94‑1 (MQ=60)
                                             TGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGG                                                   <  SRR4302545.10832093/94‑1 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                  <  SRR4302545.1471188/94‑1 (MQ=60)
                                              GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTCTTCCGGCT                                                  <  SRR4302545.6648483/94‑1 (MQ=60)
                                              GAAAGAACATCCTGTTGCTGTTCAGGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGAGT                                                  <  SRR4302545.1839438/94‑1 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                 <  SRR4302545.4244958/94‑1 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                 >  SRR4302545.6257580/1‑94 (MQ=60)
                                               AAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTG                                                 >  SRR4302545.5611338/1‑94 (MQ=60)
                                                AATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGC                                                <  SRR4302545.10331165/94‑1 (MQ=60)
                                                AATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGC                                                <  SRR4302545.2756145/94‑1 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               <  SRR4302545.7027613/94‑1 (MQ=60)
                                                 ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               >  SRR4302545.5496004/1‑94 (MQ=60)
                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                              >  SRR4302545.7265867/1‑94 (MQ=60)
                                                  TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                              <  SRR4302545.10182162/94‑1 (MQ=60)
                                                   AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             <  SRR4302545.379651/94‑1 (MQ=60)
                                                     CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTGCGGGTGCTCGAT                                           >  SRR4302545.8964896/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                          >  SRR4302545.2094008/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                          >  SRR4302545.7950019/1‑94 (MQ=60)
                                                      ATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATG                                          <  SRR4302545.2008512/94‑1 (MQ=60)
                                                      ATCATGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTGGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGATCGATG                                          >  SRR4302545.5375431/1‑94 (MQ=60)
                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                         >  SRR4302545.4115895/1‑94 (MQ=60)
                                                       TCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGG                                         <  SRR4302545.5138927/94‑1 (MQ=60)
                                                        CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                        <  SRR4302545.965170/94‑1 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCT                                       >  SRR4302545.6379889/1‑94 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       <  SRR4302545.66758/94‑1 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       >  SRR4302545.3067319/1‑94 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       <  SRR4302545.2607571/94‑1 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       >  SRR4302545.8945413/1‑94 (MQ=60)
                                                         CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       >  SRR4302545.5439089/1‑94 (MQ=60)
                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                      >  SRR4302545.8348991/1‑94 (MQ=60)
                                                          TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                      <  SRR4302545.3305029/94‑1 (MQ=60)
                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                     <  SRR4302545.4870696/94‑1 (MQ=60)
                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                     >  SRR4302545.2158705/1‑94 (MQ=60)
                                                           GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                     >  SRR4302545.1770697/1‑94 (MQ=60)
                                                            TTGCTGTTCGTGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGCATTGTCGGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                    <  SRR4302545.9920237/94‑1 (MQ=60)
                                                            TTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                    <  SRR4302545.9609366/94‑1 (MQ=60)
                                                             TGTTGTTCACGGGTCGGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302545.8665914/94‑1 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302545.6881180/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302545.1549450/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302545.1039483/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302545.996925/94‑1 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302545.3998714/1‑94 (MQ=60)
                                                             TGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302545.5826114/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                  >  SRR4302545.1768103/1‑94 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                  <  SRR4302545.4155143/94‑1 (MQ=60)
                                                              GCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAG                                  <  SRR4302545.5887042/94‑1 (MQ=60)
                                                               CTGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                 >  SRR4302545.821346/1‑94 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                >  SRR4302545.6380431/1‑94 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                <  SRR4302545.7602156/94‑1 (MQ=60)
                                                                TGTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                >  SRR4302545.32409/1‑94 (MQ=60)
                                                                 GTTCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAG                               <  SRR4302545.503706/94‑1 (MQ=60)
                                                                   TCACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCA                             >  SRR4302545.2728550/1‑94 (MQ=60)
                                                                    CACGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGCTGCTCGATGGCATCCAGCAGCAT                            >  SRR4302545.7800509/1‑94 (MQ=60)
                                                                     CCGGGTCAGTGCGTTGAAGCTCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           <  SRR4302545.2488362/94‑1 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                          >  SRR4302545.671968/1‑94 (MQ=60)
                                                                      CGGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTA                          <  SRR4302545.10874437/94‑1 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         >  SRR4302545.2026135/1‑94 (MQ=60)
                                                                       GGGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         >  SRR4302545.5517227/1‑94 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                        >  SRR4302545.4470627/1‑94 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                        <  SRR4302545.9930146/94‑1 (MQ=60)
                                                                        GGTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCC                        >  SRR4302545.5667063/1‑94 (MQ=60)
                                                                         GTCAGTGCGTTGAAGCGCA‑CGGCTTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                       <  SRR4302545.7799172/94‑1 (MQ=60)
                                                                         GTCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                       >  SRR4302545.1153597/1‑94 (MQ=60)
                                                                          TCGGTGCGTTTAAGCGCA‑CGGCGTAGCCAGAGGCACTGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                      <  SRR4302545.3565803/94‑1 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                      >  SRR4302545.5246797/1‑94 (MQ=60)
                                                                          TCAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCG                      <  SRR4302545.4394206/94‑1 (MQ=60)
                                                                           CAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                     <  SRR4302545.5484712/94‑1 (MQ=60)
                                                                           CAGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                     >  SRR4302545.10842717/1‑94 (MQ=60)
                                                                            AGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAT                    <  SRR4302545.317968/94‑1 (MQ=60)
                                                                            AGTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCCAT                    >  SRR4302545.3902653/1‑94 (MQ=60)
                                                                             GTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATT                   >  SRR4302545.2122657/1‑94 (MQ=60)
                                                                             GTGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATT                   <  SRR4302545.1760270/94‑1 (MQ=60)
                                                                              TGCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTC                  <  SRR4302545.8800839/94‑1 (MQ=60)
                                                                               GCGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCA                 <  SRR4302545.2853509/94‑1 (MQ=60)
                                                                                CGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT                >  SRR4302545.10830161/1‑94 (MQ=60)
                                                                                CGTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT                >  SRR4302545.9142963/1‑94 (MQ=60)
                                                                                 GTTGAAGCGCAGG‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT               >  SRR4302545.8922366/1‑94 (MQ=60)
                                                                                 GTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT               <  SRR4302545.283058/94‑1 (MQ=60)
                                                                                 GTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT               >  SRR4302545.6193834/1‑94 (MQ=60)
                                                                                 GTTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT               <  SRR4302545.6498048/94‑1 (MQ=60)
                                                                                  TTGAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA              <  SRR4302545.1235928/94‑1 (MQ=60)
                                                                                    GAAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTTCCGCTTCATTACG            >  SRR4302545.2343654/1‑94 (MQ=60)
                                                                                     AAGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGT           <  SRR4302545.7675106/94‑1 (MQ=60)
                                                                                      AGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGCTATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          >  SRR4302545.6374731/1‑94 (MQ=60)
                                                                                      AGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          >  SRR4302545.5550347/1‑94 (MQ=60)
                                                                                      AGCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTT          <  SRR4302545.3740096/94‑1 (MQ=60)
                                                                                       GCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         >  SRR4302545.4633056/1‑94 (MQ=60)
                                                                                       GCGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         <  SRR4302545.2404791/94‑1 (MQ=60)
                                                                                        CGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        <  SRR4302545.7851698/94‑1 (MQ=60)
                                                                                        CGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        <  SRR4302545.2566920/94‑1 (MQ=60)
                                                                                        CGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        >  SRR4302545.211717/1‑94 (MQ=60)
                                                                                        CGCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        <  SRR4302545.8545671/94‑1 (MQ=60)
                                                                                         GCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302545.5650383/94‑1 (MQ=60)
                                                                                         GCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       >  SRR4302545.8066572/1‑94 (MQ=60)
                                                                                         GCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302545.5650381/94‑1 (MQ=60)
                                                                                         GCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302545.6249101/94‑1 (MQ=60)
                                                                                         GCA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302545.10097316/94‑1 (MQ=60)
                                                                                          CA‑CGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      >  SRR4302545.3010893/1‑94 (MQ=60)
                                                                                          CA‑CGGCGTAGCCAGAGACACGGAATGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      <  SRR4302545.7860715/94‑1 (MQ=60)
                                                                                             cgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT    >  SRR4302545.7676238/3‑94 (MQ=60)
                                                                                             cgGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTT    >  SRR4302545.1502323/3‑94 (MQ=60)
                                                                                             G‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG   <  SRR4302545.2301535/94‑1 (MQ=60)
                                                                                             G‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTG   <  SRR4302545.4050670/94‑1 (MQ=60)
                                                                                               GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  <  SRR4302545.8765606/94‑1 (MQ=60)
                                                                                               |                                                                                             
TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCA‑C‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257745‑3257931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑