Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,257,838	+G	coding (2222/2241 nt)	tdcE ←	putative formate acetyltransferase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,257,837	1	.	G	100.0%	788.7 / NA	228	A741A (GCG→GCC)	tdcE	putative formate acetyltransferase 3
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (110/118); total (110/118)

Only 100 of 241 total aligned reads displayed.

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924

tttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc                                                                                          <  2:6426440/93‑1 (MQ=255)

 ttttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑g                                                                                        <  2:2878104/93‑1 (MQ=255)

  tttCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑gg                                                                                       <  2:3966440/93‑2 (MQ=255)

    tCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAc‑ggcg                                                                                     >  2:2602285/1‑90 (MQ=255)

        aCATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGc                                                                                  >  1:2774165/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                <  1:4582980/93‑1 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                >  2:1769391/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                >  1:636272/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                <  1:2255957/93‑1 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                >  1:3905581/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                >  2:3850097/1‑93 (MQ=255)

          aTCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCa                                                                                <  2:2823629/93‑1 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               <  2:5290706/93‑1 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               >  1:4580543/1‑93 (MQ=255)

           tcctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCag                                                                               >  1:4075305/1‑93 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  1:463964/1‑93 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  2:2202511/1‑93 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  1:6202418/1‑93 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             >  2:527275/1‑93 (MQ=255)

             ctccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCagag                                                                             <  1:1359156/93‑1 (MQ=255)

              tccGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAgaga                                                                            >  2:4382688/1‑93 (MQ=255)

                cGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGaca                                                                          <  2:1949429/93‑1 (MQ=255)

                 ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                                         <  2:5067110/93‑1 (MQ=255)

                 ggCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGacac                                                                         <  2:5924850/93‑1 (MQ=255)

                     tCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAt                                                                     >  1:4517808/1‑93 (MQ=255)

                       agagCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTg                                                                   <  2:5374912/93‑1 (MQ=255)

                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                <  1:2518311/93‑1 (MQ=255)

                          gcgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                <  1:514835/93‑1 (MQ=255)

                          gcgcCTGGCTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCa                                                                <  2:2316290/93‑1 (MQ=255)

                           cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                               <  1:4960467/93‑1 (MQ=255)

                           cgcCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAg                                                               <  1:306208/93‑1 (MQ=255)

                             ccTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGt                                                             <  1:1717996/93‑1 (MQ=255)

                              cTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGtt                                                            >  2:306228/1‑93 (MQ=255)

                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTa                                                           >  1:5852299/1‑93 (MQ=255)

                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTa                                                           <  1:5534552/93‑1 (MQ=255)

                               tGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTa                                                           >  2:2244708/1‑93 (MQ=255)

                                gggTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAg                                                          <  1:437478/93‑1 (MQ=255)

                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                        <  1:3121259/93‑1 (MQ=255)

                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                        >  2:4501221/1‑93 (MQ=255)

                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                        >  1:5346118/1‑93 (MQ=255)

                                  gTAAAGGTACGTGAAATAACATCCTGTTGCTGGTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGa                                                        <  1:1271630/93‑1 (MQ=255)

                                   tAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                       >  1:1075211/1‑93 (MQ=255)

                                   tAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGat                                                       <  1:1912782/93‑1 (MQ=255)

                                       ggTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttt                                                   >  1:1550900/1‑93 (MQ=255)

                                         tACGTGAAATAACATCCTGTTTCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                 <  1:1658071/93‑1 (MQ=255)

                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTTGAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAttttt                                                 <  1:263408/93‑1 (MQ=255)

                                         tACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAGGATAttttt                                                 >  2:3430537/1‑93 (MQ=255)

                                          aCGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTc                                                <  2:6199825/93‑1 (MQ=255)

                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                               <  1:3019428/93‑1 (MQ=255)

                                           cGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTcc                                               <  2:1526040/93‑1 (MQ=255)

                                            gTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCg                                              >  2:1333479/1‑93 (MQ=255)

                                             tGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCgg                                             <  2:2076018/93‑1 (MQ=255)

                                                 aTAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGc                                         <  2:3720125/93‑1 (MQ=255)

                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        >  2:896893/1‑93 (MQ=255)

                                                  tAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCt                                        >  1:583366/1‑93 (MQ=255)

                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCg                                      >  2:4956113/1‑93 (MQ=255)

                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCg                                      >  1:3806707/1‑93 (MQ=255)

                                                    aCATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCg                                      >  1:2809671/1‑93 (MQ=255)

                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  1:3277136/93‑1 (MQ=255)

                                                     cATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGa                                     <  1:5417875/93‑1 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                   <  2:5387943/93‑1 (MQ=255)

                                                       tCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATg                                   <  2:3497975/93‑1 (MQ=255)

                                                        ccTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATgg                                  <  2:3536336/93‑1 (MQ=255)

                                                         cTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGc                                 <  2:36083/93‑1 (MQ=255)

                                                          tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCa                                <  1:1024667/93‑1 (MQ=255)

                                                          tGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCa                                >  1:5581129/1‑93 (MQ=255)

                                                           gTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAt                               <  1:5076260/93‑1 (MQ=255)

                                                               cTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                           <  1:487348/93‑1 (MQ=255)

                                                               cTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcag                           <  1:2208740/93‑1 (MQ=255)

                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                          <  2:2612235/93‑1 (MQ=255)

                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                          <  1:6287445/93‑1 (MQ=255)

                                                                tGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagc                          >  1:2571300/1‑93 (MQ=255)

                                                                 gTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagca                         >  1:829104/1‑93 (MQ=255)

                                                                  ttCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagcag                        <  1:501443/93‑1 (MQ=255)

                                                                  ttCACGGGTCAGTGCGTTGAAGCCCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCcagcag                        <  1:5989486/93‑1 (MQ=255)

                                                                    cACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAgcagca                      <  2:76322/93‑1 (MQ=255)

                                                                      cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                    >  1:3341333/1‑93 (MQ=255)

                                                                      cGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAtt                    <  1:1821784/93‑1 (MQ=255)

                                                                       gggTCAGTTCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAttt                   <  1:1370880/93‑1 (MQ=255)

                                                                        ggTCAGTGAGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTc                  >  2:6285268/1‑93 (MQ=255)

                                                                         gTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTcc                 >  2:2969273/1‑93 (MQ=255)

                                                                             gTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGAt             <  1:4541382/93‑1 (MQ=255)

                                                                               gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTc           <  1:4034215/93‑1 (MQ=255)

                                                                               gCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTc           >  2:314141/1‑93 (MQ=255)

                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         >  2:5693542/1‑93 (MQ=255)

                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         <  1:2338044/93‑1 (MQ=255)

                                                                                 gTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAt         >  2:4839497/1‑93 (MQ=255)

                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        >  2:2850270/1‑93 (MQ=255)

                                                                                  ttGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAtt        <  2:1502502/93‑1 (MQ=255)

                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:3756829/93‑1 (MQ=255)

                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:1682296/93‑1 (MQ=255)

                                                                                   tGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  1:3202319/93‑1 (MQ=255)

                                                                                   tGAAGCGCACGGCGTACATAGAGACACGGATTGTCAGGTTATGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTa       <  2:5005870/93‑1 (MQ=37)

                                                                                    gAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTac      <  2:405001/93‑1 (MQ=255)

                                                                                      aGCGCACGGCGTAGCCAGAGTCACGGATTGTCAGGTTAGCATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATGTCCCGATTCATTacgt    <  2:1163404/93‑1 (MQ=37)

                                                                                       gcgcACGGCGTAGCCAGAGACACGGATTGTCCGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgtt   >  2:5090516/1‑93 (MQ=255)

                                                                                       gcgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgtt   >  2:1053689/1‑93 (MQ=255)

                                                                                       gcgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgtt   <  2:4778313/93‑1 (MQ=255)

                                                                                        cgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg  >  1:6110323/1‑93 (MQ=255)

                                                                                        cgcACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTacgttg  <  1:2747815/93‑1 (MQ=255)

TTTTTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG  >  NC_000913/3257745‑3257924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257748‑3257931

TTCATACATCATCCGGCTACAGAGCGCCTTGGTAAAGGTACGTGAAATAACTTCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑Ggcg                                                                                            <  SRR4302537.642985/94‑4 (MQ=60)

  CATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTA                                                                                           >  SRR4302537.407807/1‑94 (MQ=60)

   ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                          >  SRR4302537.5061506/1‑94 (MQ=60)

   ATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAG                                                                                          <  SRR4302537.5042355/94‑1 (MQ=60)

    TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                         >  SRR4302537.2793558/1‑94 (MQ=60)

    TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                         >  SRR4302537.2821544/1‑94 (MQ=60)

    TACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGC                                                                                         >  SRR4302537.4040648/1‑94 (MQ=60)

      CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCA                                                                                       <  SRR4302537.6085050/94‑1 (MQ=60)

      CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCA                                                                                       >  SRR4302537.3932887/1‑94 (MQ=60)

      CATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCA                                                                                       >  SRR4302537.640598/1‑94 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      >  SRR4302537.4612229/1‑94 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      <  SRR4302537.2271880/94‑1 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      <  SRR4302537.4614678/94‑1 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      >  SRR4302537.308446/1‑94 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      >  SRR4302537.4103760/1‑94 (MQ=60)

       ATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAG                                                                                      >  SRR4302537.1292812/1‑94 (MQ=60)

         CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                    >  SRR4302537.6245166/1‑94 (MQ=60)

         CCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAG                                                                                    >  SRR4302537.467157/1‑94 (MQ=60)

          CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                   <  SRR4302537.1378033/94‑1 (MQ=60)

          CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                   <  SRR4302537.1368622/94‑1 (MQ=60)

          CTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGA                                                                                   >  SRR4302537.5393136/1‑94 (MQ=60)

           TCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGAC                                                                                  >  SRR4302537.5569594/1‑94 (MQ=60)

             CGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACAC                                                                                <  SRR4302537.5375634/94‑1 (MQ=60)

              GGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACG                                                                               >  SRR4302537.1584223/1‑94 (MQ=60)

               GCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGG                                                                              <  SRR4302537.3240909/94‑1 (MQ=60)

                CGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGA                                                                             >  SRR4302537.5292862/1‑94 (MQ=60)

                 GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                            >  SRR4302537.4549060/1‑94 (MQ=60)

                 GTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGAT                                                                            <  SRR4302537.807074/94‑1 (MQ=60)

                  TCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATT                                                                           >  SRR4302537.2623971/1‑94 (MQ=60)

                    AGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGT                                                                         >  SRR4302537.4641648/1‑94 (MQ=60)

                     GAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTC                                                                        >  SRR4302537.2771970/1‑94 (MQ=60)

                       GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302537.1074676/94‑1 (MQ=60)

                       GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302537.2536001/94‑1 (MQ=60)

                       GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302537.518376/94‑1 (MQ=60)

                       GCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAG                                                                      <  SRR4302537.6016564/94‑1 (MQ=60)

                        CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                     >  SRR4302537.1147143/1‑94 (MQ=60)

                        CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                     >  SRR4302537.4146203/1‑94 (MQ=60)

                        CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                     <  SRR4302537.308329/94‑1 (MQ=60)

                        CGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGG                                                                     <  SRR4302537.4994660/94‑1 (MQ=60)

                         GCCTGGGTAAATGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGT                                                                    >  SRR4302537.3346233/1‑94 (MQ=60)

                          CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                   <  SRR4302537.4507976/94‑1 (MQ=60)

                          CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                   <  SRR4302537.1729984/94‑1 (MQ=60)

                          CCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTT                                                                   >  SRR4302537.6064894/1‑94 (MQ=60)

                           CTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTA                                                                  >  SRR4302537.5892446/1‑94 (MQ=60)

                            TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 >  SRR4302537.1756169/1‑94 (MQ=60)

                            TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 <  SRR4302537.5572331/94‑1 (MQ=60)

                            TGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAG                                                                 >  SRR4302537.5045077/1‑94 (MQ=60)

                             GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTGAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                                >  SRR4302537.4576943/1‑94 (MQ=60)

                             GGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGG                                                                <  SRR4302537.440527/94‑1 (MQ=60)

                              GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               >  SRR4302537.1502327/1‑94 (MQ=60)

                              GGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGA                                                               >  SRR4302537.5382745/1‑94 (MQ=60)

                               GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                              >  SRR4302537.1082619/1‑94 (MQ=60)

                               GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                              <  SRR4302537.3143087/94‑1 (MQ=60)

                               GTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGATAGGAT                                                              >  SRR4302537.3910629/1‑94 (MQ=60)

                               GTAAAGGTACGTGAAATAACATCCTGTTGCTGGTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAT                                                              <  SRR4302537.1280469/94‑1 (MQ=60)

                                TAAAGGTACGTGAAATAACATCCTGTTGCTGTTTACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             >  SRR4302537.2816315/1‑94 (MQ=60)

                                TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             <  SRR4302537.4613469/94‑1 (MQ=60)

                                TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             <  SRR4302537.4880281/94‑1 (MQ=60)

                                TAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATA                                                             <  SRR4302537.1926061/94‑1 (MQ=60)

                                 AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            <  SRR4302537.6307197/94‑1 (MQ=60)

                                 AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            >  SRR4302537.4416743/1‑94 (MQ=60)

                                 AAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATAT                                                            <  SRR4302537.2441260/94‑1 (MQ=60)

                                   AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTT                                                          >  SRR4302537.1561723/1‑94 (MQ=60)

                                   AGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGAAATTT                                                          >  SRR4302537.1357800/1‑94 (MQ=60)

                                    GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                         >  SRR4302537.3811484/1‑94 (MQ=60)

                                    GGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                         <  SRR4302537.39849/94‑1 (MQ=60)

                                    AGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTT                                                         <  SRR4302537.2875645/94‑1 (MQ=60)

                                      TACGTGAAATAACATCCTGTTTCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                       <  SRR4302537.1669692/94‑1 (MQ=60)

                                      TACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTTGAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTC                                                       <  SRR4302537.265262/94‑1 (MQ=60)

                                        CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302537.933962/94‑1 (MQ=60)

                                        CGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCG                                                     <  SRR4302537.3040477/94‑1 (MQ=60)

                                         GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                    <  SRR4302537.3066866/94‑1 (MQ=60)

                                         GTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGG                                                    >  SRR4302537.1912123/1‑94 (MQ=60)

                                           GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGT                                                  >  SRR4302537.2579946/1‑94 (MQ=60)

                                           GAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGGTAGGATATTTTTACGGGG                                                  >  SRR4302537.2247971/1‑94 (MQ=60)

                                              ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               >  SRR4302537.587354/1‑94 (MQ=60)

                                              ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               <  SRR4302537.5598764/94‑1 (MQ=60)

                                              ATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCT                                               >  SRR4302537.5473416/1‑94 (MQ=60)

                                               TAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTC                                              <  SRR4302537.168372/94‑1 (MQ=60)

                                                AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             >  SRR4302537.2103894/1‑94 (MQ=60)

                                                AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             >  SRR4302537.2829312/1‑94 (MQ=60)

                                                AACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCG                                             >  SRR4302537.3833397/1‑94 (MQ=60)

                                                 ACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGA                                            <  SRR4302537.3059823/94‑1 (MQ=60)

                                                  CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                           <  SRR4302537.5454806/94‑1 (MQ=60)

                                                  CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                           <  SRR4302537.3300262/94‑1 (MQ=60)

                                                  CATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGAT                                           >  SRR4302537.1420244/1‑94 (MQ=60)

                                                     CCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGC                                        >  SRR4302537.2387624/1‑94 (MQ=60)

                                                      CTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCA                                       >  SRR4302537.5619276/1‑94 (MQ=60)

                                                       TGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCAT                                      <  SRR4302537.1031715/94‑1 (MQ=60)

                                                        GTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATC                                     <  SRR4302537.5111203/94‑1 (MQ=60)

                                                         TTGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCC                                    <  SRR4302537.3184045/94‑1 (MQ=60)

                                                          TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302537.3451796/94‑1 (MQ=60)

                                                          TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   <  SRR4302537.3149092/94‑1 (MQ=60)

                                                          TGCTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCA                                   >  SRR4302537.4960373/1‑94 (MQ=60)

                                                            CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                 <  SRR4302537.490695/94‑1 (MQ=60)

                                                            CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                 <  SRR4302537.2224311/94‑1 (MQ=60)

                                                            CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                 >  SRR4302537.2589311/1‑94 (MQ=60)

                                                            CTGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGC                                 >  SRR4302537.847075/1‑94 (MQ=60)

                                                             TGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                <  SRR4302537.6330544/94‑1 (MQ=60)

                                                             TGTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCA                                >  SRR4302537.834780/1‑94 (MQ=60)

                                                              GTTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAG                               >  SRR4302537.5887904/1‑94 (MQ=60)

                                                               TTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                              >  SRR4302537.6364231/1‑94 (MQ=60)

                                                               TTCACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                              <  SRR4302537.504891/94‑1 (MQ=60)

                                                               TTCACGGGTCAGTGCGTTGAAGCCCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGC                              <  SRR4302537.6030620/94‑1 (MQ=60)

                                                                 CACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCAT                            >  SRR4302537.2194085/1‑94 (MQ=60)

                                                                  ACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           >  SRR4302537.4592351/1‑94 (MQ=60)

                                                                  ACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           >  SRR4302537.3364989/1‑94 (MQ=60)

                                                                  ACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           <  SRR4302537.6318659/94‑1 (MQ=60)

                                                                  ACGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATT                           <  SRR4302537.5803092/94‑1 (MQ=60)

                                                                   CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                          <  SRR4302537.1834486/94‑1 (MQ=60)

                                                                   CGGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTT                          <  SRR4302537.2602358/94‑1 (MQ=60)

                                                                    GGGTCAGTTCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         <  SRR4302537.1380430/94‑1 (MQ=60)

                                                                    GGGTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTC                         >  SRR4302537.5761439/1‑94 (MQ=60)

                                                                      GTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                       <  SRR4302537.4558938/94‑1 (MQ=60)

                                                                      GTCAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCC                       <  SRR4302537.500234/94‑1 (MQ=60)

                                                                        CAGTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGA                     >  SRR4302537.6163707/1‑94 (MQ=60)

                                                                          GTGCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATT                   <  SRR4302537.4572818/94‑1 (MQ=60)

                                                                            GCGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCA                 <  SRR4302537.4062453/94‑1 (MQ=60)

                                                                             CGTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCAT                <  SRR4302537.5114891/94‑1 (MQ=60)

                                                                              GTTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATT               <  SRR4302537.2354526/94‑1 (MQ=60)

                                                                               TTGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTA              <  SRR4302537.2306490/94‑1 (MQ=60)

                                                                                TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC             <  SRR4302537.4097783/94‑1 (MQ=60)

                                                                                TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC             <  SRR4302537.3224832/94‑1 (MQ=60)

                                                                                TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC             >  SRR4302537.2710919/1‑94 (MQ=60)

                                                                                TGAAGCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTAC             <  SRR4302537.179447/94‑1 (MQ=60)

                                                                                    GCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         >  SRR4302537.6152316/1‑94 (MQ=60)

                                                                                    GCGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTG         <  SRR4302537.4886136/94‑1 (MQ=60)

                                                                                     CGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        >  SRR4302537.1728392/1‑94 (MQ=60)

                                                                                     CGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        >  SRR4302537.2639574/1‑94 (MQ=60)

                                                                                     CGCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGA        <  SRR4302537.2766979/94‑1 (MQ=60)

                                                                                      GCACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGAC       <  SRR4302537.5230742/94‑1 (MQ=60)

                                                                                       CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      <  SRR4302537.5019915/94‑1 (MQ=60)

                                                                                       CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      >  SRR4302537.640856/1‑94 (MQ=60)

                                                                                       CACGGCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACG      <  SRR4302537.577331/94‑1 (MQ=60)

                                                                                        ccgGCGTAGCCAGAGACACGGATTGTCAGGTTAGTATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGT     <  SRR4302537.513963/91‑1 (MQ=60)

                                                                                           GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  <  SRR4302537.2005604/94‑1 (MQ=60)

TTCATACATCCTCCGGCGTCAGAGCGCCTGGGTAAAGGTACGTGAAATAACATCCTGTTGCTGTTCACGGGTCAGTGCGTTGAAGCGCAC‑GCGTAGCCAGAGACACGGATTGTCAGGTTAGGATATTTTTCCGGGTGCTCGATGGCATCCAGCAGCATTTCCCGATTCATTACGTTGACGTTGA  >  NC_000913/3257748‑3257931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`