Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,430,453 +C coding (745/747 nt) smf ← putative Rossmann‑fold nucleotide‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,430,4531.C99.3% 1470.4 / 0.3 425coding (745/747 nt)smfputative Rossmann‑fold nucleotide‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (242/180);  total (243/182)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 480 total aligned reads displayed.

AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT  >  NC_000913/3430360‑3430540
                                                                                              |                                                                                       
aGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAgg                                                                                          <  2:2293035/94‑1 (MQ=255)
aGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAgg                                                                                          <  1:2017247/94‑1 (MQ=255)
 gAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggc                                                                                         >  1:9192381/1‑93 (MQ=255)
 gAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggc                                                                                         <  2:3784018/94‑2 (MQ=255)
  aaTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAggca                                                                                        >  2:3105266/1‑92 (MQ=255)
   aTTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaagg‑caa                                                                                      <  2:5541426/94‑1 (MQ=255)
   aTTTCTTCCGGCTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTggcaagg‑caa                                                                                      <  2:1035649/94‑1 (MQ=255)
       cttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑caaaaac                                                                                  >  1:1752815/1‑92 (MQ=255)
        ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACt                                                                                  >  1:9815867/1‑94 (MQ=255)
        ttcCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACt                                                                                  <  2:3247818/94‑1 (MQ=255)
          ccGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtc                                                                                >  2:5748686/1‑94 (MQ=255)
           cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtct                                                                               >  2:9687484/1‑94 (MQ=255)
           cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtct                                                                               >  2:2629973/1‑94 (MQ=255)
           cGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtct                                                                               <  1:22568/94‑1 (MQ=255)
            ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtcta                                                                              >  2:1796978/1‑93 (MQ=255)
            ggTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAAATtctc                                                                              >  2:6272282/1‑94 (MQ=255)
             gTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTtctca                                                                             <  1:6604509/94‑2 (MQ=255)
               tCCGTCACAAGAGTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcc                                                                           >  1:10445808/1‑94 (MQ=255)
                 cGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGcccc                                                                         >  2:7459180/1‑94 (MQ=255)
                   tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTg                                                                       <  1:6931465/94‑1 (MQ=255)
                   tCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTg                                                                       >  1:5444389/1‑94 (MQ=255)
                    cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTTGCACGGCAAAAACTTCTCGCCCCTGc                                                                      >  1:7634598/1‑94 (MQ=255)
                    cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                      >  2:9118702/1‑94 (MQ=255)
                    cacaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGc                                                                      <  1:10169175/94‑1 (MQ=255)
                     acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                     >  1:4857414/1‑94 (MQ=255)
                     acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                     <  2:9857238/94‑1 (MQ=255)
                     acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                     <  1:10281419/94‑1 (MQ=255)
                     acaAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCt                                                                     <  2:3412755/94‑1 (MQ=255)
                        aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCaa                                                                  >  2:2663321/1‑94 (MQ=255)
                        aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCaa                                                                  <  1:7188804/94‑1 (MQ=255)
                        aGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCaa                                                                  <  2:446512/94‑1 (MQ=255)
                          aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgc                                                                <  2:9439367/94‑1 (MQ=255)
                          aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgc                                                                <  2:873776/94‑1 (MQ=255)
                          aaTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgc                                                                >  1:1172429/1‑94 (MQ=255)
                           aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgcg                                                               >  1:9498110/1‑94 (MQ=255)
                           aTCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAgcg                                                               <  2:689832/94‑1 (MQ=255)
                             cGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGca                                                             >  2:10477639/1‑94 (MQ=255)
                                  cTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACg                                                        <  1:8661230/94‑1 (MQ=255)
                                    tGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTg                                                      >  2:10081941/1‑94 (MQ=255)
                                     gTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGc                                                     >  1:451031/1‑94 (MQ=255)
                                      ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCt                                                    >  2:6733678/1‑94 (MQ=255)
                                      ttttATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCt                                                    >  1:4295411/1‑94 (MQ=255)
                                        ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGt                                                  >  2:4523376/1‑94 (MQ=255)
                                        ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGt                                                  >  2:4031151/1‑94 (MQ=255)
                                        ttattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGt                                                  >  1:3236695/1‑94 (MQ=255)
                                         tattaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTc                                                 >  1:2076875/1‑94 (MQ=255)
                                          attaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCa                                                >  1:2451098/1‑94 (MQ=255)
                                           ttaACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCAc                                               >  2:2077263/1‑94 (MQ=255)
                                             aaCGAGTGAGGCCCTTCGCTTCCCGCATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                             <  2:727191/94‑1 (MQ=255)
                                             aaCCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCa                                             <  1:4197209/94‑1 (MQ=255)
                                               ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                           <  2:8462243/94‑1 (MQ=255)
                                               ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                           <  1:8745008/94‑1 (MQ=255)
                                               ccAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGc                                           >  2:1580537/1‑94 (MQ=255)
                                                cAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCg                                          <  1:4639376/94‑1 (MQ=255)
                                                   tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAc                                       >  2:8914117/1‑94 (MQ=255)
                                                   tGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAc                                       <  2:4890989/94‑1 (MQ=255)
                                                    gAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAAcc                                      >  1:1336485/1‑94 (MQ=255)
                                                     aGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCa                                     >  1:3678162/1‑94 (MQ=255)
                                                      ggCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCAc                                    >  2:10421913/1‑94 (MQ=255)
                                                        cccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTa                                  >  2:4395917/1‑94 (MQ=255)
                                                         ccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                 <  1:6438624/94‑1 (MQ=255)
                                                         ccTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTAc                                 >  2:8295342/1‑94 (MQ=255)
                                                          cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                                >  2:7061112/1‑94 (MQ=255)
                                                          cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                                >  1:80989/1‑94 (MQ=255)
                                                          cTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACg                                >  1:7869372/1‑94 (MQ=255)
                                                          cTTCGCTTCCCGAATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTTCTGTCACCAGCGAACCACTACg                                <  1:1563372/94‑1 (MQ=255)
                                                           ttCGCTTCCCGGATTCCCTATTGGACCTGGTAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                               >  1:6531095/1‑94 (MQ=255)
                                                           ttCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACga                               >  2:2956036/1‑93 (MQ=255)
                                                           ttCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGc                               >  1:5219929/1‑94 (MQ=255)
                                                            tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCa                              >  2:60821/1‑94 (MQ=255)
                                                            tCGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCa                              >  2:8818829/1‑94 (MQ=255)
                                                             cGCTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCaa                             >  1:5669115/1‑94 (MQ=255)
                                                               cTTCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTGACCAGCGAACCACTACGCagag                           >  2:5093617/1‑94 (MQ=255)
                                                                 tCCCGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGcc                         <  2:1259096/94‑1 (MQ=255)
                                                                  cccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                        <  1:879605/94‑1 (MQ=255)
                                                                  cccGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCg                        <  1:6703096/94‑1 (MQ=255)
                                                                    cGGATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCt                      >  1:288730/1‑94 (MQ=255)
                                                                     ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCtt                     <  1:3924793/94‑1 (MQ=255)
                                                                     ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCtt                     <  1:8361867/94‑1 (MQ=255)
                                                                     ggATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCtt                     >  1:1004668/1‑94 (MQ=255)
                                                                      gATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTc                    >  1:187674/1‑94 (MQ=255)
                                                                      gATTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACCACGCAAAGCCGCCTc                    >  1:6817944/1‑94 (MQ=255)
                                                                       aTTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                   >  1:6092373/1‑94 (MQ=255)
                                                                       aTTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                   >  1:5079132/1‑94 (MQ=255)
                                                                       aTTCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcc                   <  2:5226226/94‑1 (MQ=255)
                                                                        ttCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTcca                  >  2:10583892/1‑94 (MQ=255)
                                                                         tCCCTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccac                 >  1:1424776/1‑94 (MQ=255)
                                                                          cccTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacc                <  1:2570085/94‑1 (MQ=255)
                                                                           ccTATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccacca               >  1:5874211/1‑94 (MQ=255)
                                                                             tATTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacc             >  2:9060949/1‑94 (MQ=255)
                                                                              aTTGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTccaccacca            <  1:6994368/94‑1 (MQ=255)
                                                                                tGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGt          >  1:8177767/1‑94 (MQ=255)
                                                                                tGGACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGt          >  2:9242950/1‑94 (MQ=255)
                                                                                 ggACCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTa         >  2:6390954/1‑94 (MQ=255)
                                                                                   aCCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTaca       >  2:4611549/1‑94 (MQ=255)
                                                                                   aCCTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTaca       <  1:744398/94‑1 (MQ=255)
                                                                                    ccTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTacac      <  1:2972247/94‑1 (MQ=255)
                                                                                     cTGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACAcc     <  1:2707015/94‑1 (MQ=255)
                                                                                      tGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt    >  2:8009940/1‑94 (MQ=255)
                                                                                      tGGCAAGGCAAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCt    >  1:1683464/1‑94 (MQ=255)
                                                                                              |                                                                                       
AGAATTTCTTCCGGTTCCGTCACAAGAATCGCACCTTGTTTTATTAACCAGTGAGGCCCTTCGCTTCCCGGATTCCCTATTGGACCTGGCAAGG‑AAAAACTTCTCGCCCCTGCTCAAGCGCACAACGTGCTGTCACCAGCGAACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTT  >  NC_000913/3430360‑3430540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 265 total aligned reads displayed.
AAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  NC_000913/3434159‑3434345
                                                                                             |                                                                                             
AAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGA                                                                                               <  SRR4302545.9724063/94‑1 (MQ=60)
 AATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGAT                                                                                              <  SRR4302545.1928038/94‑1 (MQ=60)
 AATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGAT                                                                                              <  SRR4302545.10394925/94‑1 (MQ=60)
  ATTATCATTCTGGGTGCCGGTCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATG                                                                                             <  SRR4302545.747071/94‑1 (MQ=60)
  ATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATG                                                                                             >  SRR4302545.7280601/1‑94 (MQ=60)
  ATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATG                                                                                             <  SRR4302545.10785406/94‑1 (MQ=60)
   TTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGA                                                                                            <  SRR4302545.7911237/94‑1 (MQ=60)
   TTATCATTCTGGGTGCCGACCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGA                                                                                            >  SRR4302545.9537192/1‑94 (MQ=60)
    TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           >  SRR4302545.2528384/1‑94 (MQ=60)
    TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           >  SRR4302545.10293483/1‑94 (MQ=60)
    TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           >  SRR4302545.8736933/1‑94 (MQ=60)
    TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           >  SRR4302545.2145675/1‑94 (MQ=60)
    TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           <  SRR4302545.5565045/94‑1 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          <  SRR4302545.10454559/94‑1 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          >  SRR4302545.6417208/1‑94 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          <  SRR4302545.3661130/94‑1 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          >  SRR4302545.4767532/1‑94 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          >  SRR4302545.7917770/1‑94 (MQ=60)
     ATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGC                                                                                          <  SRR4302545.121322/94‑1 (MQ=60)
      TCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCG                                                                                         <  SRR4302545.5853577/94‑1 (MQ=60)
       CATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGT                                                                                        >  SRR4302545.166249/1‑94 (MQ=60)
       CATTCTGGGGGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGCACAACGATATTACTGTTGTCGATACCAACGATGAGCGT                                                                                        >  SRR4302545.6582369/1‑94 (MQ=60)
        ATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTC                                                                                       <  SRR4302545.210751/94‑1 (MQ=60)
        ATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTC                                                                                       >  SRR4302545.10105099/1‑94 (MQ=60)
         TTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCT                                                                                      <  SRR4302545.332281/94‑1 (MQ=60)
          TCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTG                                                                                     <  SRR4302545.2573412/94‑1 (MQ=60)
          TCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTG                                                                                     >  SRR4302545.4655576/1‑94 (MQ=60)
           CTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGC                                                                                    >  SRR4302545.7960604/1‑94 (MQ=60)
           CTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGC                                                                                    >  SRR4302545.10868563/1‑94 (MQ=60)
            TGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCG                                                                                   >  SRR4302545.1202970/1‑94 (MQ=60)
             GGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGG                                                                                  <  SRR4302545.5061539/94‑1 (MQ=60)
             GGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGG                                                                                  <  SRR4302545.865124/94‑1 (MQ=60)
             GGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGG                                                                                  <  SRR4302545.4469283/94‑1 (MQ=60)
              GGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATTCCAACGATGAGCGTCTGCGGA                                                                                 <  SRR4302545.2885159/94‑1 (MQ=60)
               GTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAC                                                                                >  SRR4302545.8660851/1‑94 (MQ=60)
                TGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACC                                                                               >  SRR4302545.9832055/1‑94 (MQ=60)
                TGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACC                                                                               <  SRR4302545.6558801/94‑1 (MQ=60)
                TGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACC                                                                               >  SRR4302545.8476653/1‑94 (MQ=60)
                 GCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTTGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCT                                                                              >  SRR4302545.6872240/1‑94 (MQ=60)
                 GCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCT                                                                              <  SRR4302545.10829994/94‑1 (MQ=60)
                  CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             >  SRR4302545.487318/1‑94 (MQ=60)
                  CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             >  SRR4302545.3579120/1‑94 (MQ=60)
                  CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             <  SRR4302545.577548/94‑1 (MQ=60)
                  CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             >  SRR4302545.2761518/1‑94 (MQ=60)
                  CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             >  SRR4302545.1918812/1‑94 (MQ=60)
                   ctgccagtgtgcCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTA                                                                            <  SRR4302545.5350905/82‑1 (MQ=60)
                   CGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTA                                                                            >  SRR4302545.6312212/1‑94 (MQ=60)
                   CGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTA                                                                            <  SRR4302545.3430711/94‑1 (MQ=60)
                   CGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTA                                                                            <  SRR4302545.10093716/94‑1 (MQ=60)
                   CGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACCATGAGCGTCTGCGGGCCTTA                                                                            >  SRR4302545.2039270/1‑94 (MQ=60)
                   CGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATAGTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTA                                                                            <  SRR4302545.5350907/94‑1 (MQ=60)
                    GGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTAC                                                                           >  SRR4302545.10135186/1‑94 (MQ=60)
                     GCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACA                                                                          >  SRR4302545.10096070/1‑94 (MQ=60)
                      CCAGGTTGGCGGCACACTGGCGGAAATCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302545.7842725/94‑1 (MQ=60)
                      CCAGGTTGGCGGCACACTGGCGGAAACCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302545.10758273/94‑1 (MQ=60)
                      CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302545.2169253/94‑1 (MQ=60)
                      CCAGATTGGCGGCACACTGGCGGAAATCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302545.7842724/94‑1 (MQ=60)
                       CAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGG                                                                        >  SRR4302545.5359917/1‑94 (MQ=60)
                       CAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGG                                                                        >  SRR4302545.4927970/1‑94 (MQ=60)
                       CAGGTTGGCGGCAAACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGG                                                                        >  SRR4302545.1253514/1‑94 (MQ=60)
                        AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGCACAACGCTCTTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTCACACGA                                                                       >  SRR4302545.9517590/1‑94 (MQ=60)
                        AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302545.6115456/1‑94 (MQ=60)
                        AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302545.9517589/1‑94 (MQ=60)
                        AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302545.3502172/1‑94 (MQ=60)
                        AGGTTGGCGGCACACTGGCGGAAAACCTGATTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGCCCTTACAGGC                                                                       >  SRR4302545.2056777/1‑94 (MQ=60)
                         GGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGAT                                                                      <  SRR4302545.7241543/94‑1 (MQ=60)
                         GGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGAT                                                                      <  SRR4302545.5135286/94‑1 (MQ=60)
                          GTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATA                                                                     <  SRR4302545.739620/94‑1 (MQ=60)
                          GTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATA                                                                     >  SRR4302545.7340946/1‑94 (MQ=60)
                             GGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAAT                                                                  <  SRR4302545.2176959/94‑1 (MQ=60)
                              GCGGTACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTTGCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATT                                                                 <  SRR4302545.5895100/94‑1 (MQ=60)
                              GCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATT                                                                 <  SRR4302545.8928004/94‑1 (MQ=60)
                              GCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATT                                                                 <  SRR4302545.6774131/94‑1 (MQ=60)
                               CGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTT                                                                <  SRR4302545.3523363/94‑1 (MQ=60)
                                  CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             <  SRR4302545.3292654/94‑1 (MQ=60)
                                  CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             <  SRR4302545.8533535/94‑1 (MQ=60)
                                  CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             >  SRR4302545.1794408/1‑94 (MQ=60)
                                  CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             >  SRR4302545.9871412/1‑94 (MQ=60)
                                   ACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACC                                                            <  SRR4302545.776212/94‑1 (MQ=60)
                                     ACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTG                                                          <  SRR4302545.3233452/94‑1 (MQ=60)
                                     ACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTG                                                          <  SRR4302545.2024059/94‑1 (MQ=60)
                                     ACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTG                                                          <  SRR4302545.4076213/94‑1 (MQ=60)
                                     ACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTG                                                          <  SRR4302545.3894213/94‑1 (MQ=60)
                                      CTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGC                                                         >  SRR4302545.8749738/1‑94 (MQ=60)
                                      CTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGC                                                         <  SRR4302545.7508600/94‑1 (MQ=60)
                                       TGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCG                                                        <  SRR4302545.3604327/94‑1 (MQ=60)
                                       TGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCG                                                        >  SRR4302545.2519381/1‑94 (MQ=60)
                                        GGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGG                                                       <  SRR4302545.4067585/94‑1 (MQ=60)
                                          CGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGT                                                     >  SRR4302545.7245303/1‑94 (MQ=60)
                                           GGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    <  SRR4302545.3511056/94‑1 (MQ=60)
                                           GGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    >  SRR4302545.2634616/1‑94 (MQ=60)
                                           GGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    <  SRR4302545.3046056/94‑1 (MQ=60)
                                           GAAAAACCTGGTTGGCGAGAACCACGATATTATTGTTGTCGATACCAACGATGAGCGTCTGTGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    <  SRR4302545.3046323/94‑1 (MQ=60)
                                            GAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCG                                                   >  SRR4302545.7528607/1‑94 (MQ=60)
                                            GAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCG                                                   >  SRR4302545.4282262/1‑94 (MQ=60)
                                            GAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCG                                                   <  SRR4302545.4464969/94‑1 (MQ=60)
                                              AAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTG                                                 >  SRR4302545.8881949/1‑94 (MQ=60)
                                              AAACCTGGGTGGCGAGAACAACGATATTACTGATGTCGATAACAACGATGGGCGTCTGCCGACCTAACAGGATAAAATTGAGCTGTGGGGCGTG                                                 >  SRR4302545.7165245/1‑94 (MQ=36)
                                               AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                >  SRR4302545.5370420/1‑94 (MQ=60)
                                               AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                >  SRR4302545.4958382/1‑94 (MQ=60)
                                               AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                <  SRR4302545.1703904/94‑1 (MQ=60)
                                                ACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCA                                               <  SRR4302545.4645901/94‑1 (MQ=60)
                                                ACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCA                                               <  SRR4302545.7598284/94‑1 (MQ=60)
                                                ACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCA                                               <  SRR4302545.4051279/94‑1 (MQ=60)
                                                 CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              >  SRR4302545.10747671/1‑94 (MQ=60)
                                                 CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              <  SRR4302545.9843855/94‑1 (MQ=60)
                                                 CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              <  SRR4302545.2788172/94‑1 (MQ=60)
                                                 CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              >  SRR4302545.4271801/1‑94 (MQ=60)
                                                  CTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGG                                             >  SRR4302545.235758/1‑94 (MQ=60)
                                                  CTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGG                                             <  SRR4302545.3006520/94‑1 (MQ=60)
                                                  CTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGG                                             >  SRR4302545.8196902/1‑94 (MQ=60)
                                                   TGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGG                                            <  SRR4302545.5147732/94‑1 (MQ=60)
                                                    GGATGGCGAGAACAACTATATTACTGTTGTCGATTCCAACAATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGG                                           <  SRR4302545.3377983/94‑1 (MQ=60)
                                                     GTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGC                                          <  SRR4302545.635057/94‑1 (MQ=60)
                                                       TGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAT                                        >  SRR4302545.2832424/1‑94 (MQ=60)
                                                        GGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATG                                       <  SRR4302545.2193681/94‑1 (MQ=60)
                                                         GCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGG                                      >  SRR4302545.2239361/1‑94 (MQ=60)
                                                            AGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTC                                   >  SRR4302545.8515999/1‑94 (MQ=60)
                                                            AGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTC                                   >  SRR4302545.2931293/1‑94 (MQ=60)
                                                            AGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTC                                   >  SRR4302545.9716886/1‑94 (MQ=60)
                                                            AGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTC                                   <  SRR4302545.2340328/94‑1 (MQ=60)
                                                              AACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTC                                 <  SRR4302545.382218/94‑1 (MQ=60)
                                                               ACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCA                                <  SRR4302545.9145912/94‑1 (MQ=60)
                                                                CAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAT                               >  SRR4302545.2306352/1‑94 (MQ=60)
                                                                CAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAT                               <  SRR4302545.353497/94‑1 (MQ=60)
                                                                CAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAT                               <  SRR4302545.5204695/94‑1 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             <  SRR4302545.7558708/94‑1 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             <  SRR4302545.7780700/94‑1 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             >  SRR4302545.5592142/1‑94 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             <  SRR4302545.7147968/94‑1 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             <  SRR4302545.8320552/94‑1 (MQ=60)
                                                                  ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             <  SRR4302545.1773308/94‑1 (MQ=60)
                                                                    GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           <  SRR4302545.4950945/94‑1 (MQ=60)
                                                                    GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           >  SRR4302545.4467500/1‑94 (MQ=60)
                                                                     ATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          >  SRR4302545.10111656/1‑94 (MQ=60)
                                                                     ATATGACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          <  SRR4302545.7280782/94‑1 (MQ=60)
                                                                      TATTACTGTTGTGGATACCAACGATGAGCGTCTGCGGGCATTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGC                         >  SRR4302545.6299403/1‑94 (MQ=60)
                                                                      TATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGC                         >  SRR4302545.10918822/1‑94 (MQ=60)
                                                                       ATTACTGTTGTCGATACCAACTATGAGCGTCTGCGGCCCTTACAGGATAAATTTGACCTGCTGGTCGTGCAGGGGCATGGCTCTCATtcactct                        >  SRR4302545.9927382/1‑87 (MQ=60)
                                                                       ATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        >  SRR4302545.7540142/1‑94 (MQ=60)
                                                                       ATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        <  SRR4302545.9825156/94‑1 (MQ=60)
                                                                       ATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        <  SRR4302545.3710479/94‑1 (MQ=60)
                                                                       ATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        <  SRR4302545.3201000/94‑1 (MQ=60)
                                                                       ATTACTGTTGACGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        >  SRR4302545.7246383/1‑94 (MQ=60)
                                                                        TTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGT                       >  SRR4302545.2762429/1‑94 (MQ=60)
                                                                        TTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGT                       <  SRR4302545.2443587/94‑1 (MQ=60)
                                                                         TACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGTGGCATGGCTCTCATCCACGCGTA                      >  SRR4302545.5987644/1‑94 (MQ=60)
                                                                         TACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTA                      >  SRR4302545.10941340/1‑94 (MQ=60)
                                                                         TACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTA                      >  SRR4302545.6229470/1‑94 (MQ=60)
                                                                          ACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAT                     <  SRR4302545.4768712/94‑1 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    <  SRR4302545.10267508/94‑1 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    <  SRR4302545.1524181/94‑1 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    <  SRR4302545.8661187/94‑1 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    >  SRR4302545.2889661/1‑94 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    <  SRR4302545.7705185/94‑1 (MQ=60)
                                                                           CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    >  SRR4302545.4779469/1‑94 (MQ=60)
                                                                           CTGTTGTAGATACCAACGATGAGCGTCAGCGGACCTTACAGGATAAATTTGACCTGCTGTTCGTGCAGGGTCATGGCTCTCATCCTCGCGTATT                    <  SRR4302545.6020680/94‑1 (MQ=60)
                                                                            TGTTGTCTATACGAACGATGAGCGCCTGCGGGCCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTG                   <  SRR4302545.5877477/94‑1 (MQ=60)
                                                                             GTTGTCGATACCAACGGTGAGCGTCTGCGGACCGTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTTC                  >  SRR4302545.10543052/1‑94 (MQ=60)
                                                                             GTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGG                  >  SRR4302545.9180040/1‑94 (MQ=60)
                                                                             GTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGC                  <  SRR4302545.2776710/94‑1 (MQ=60)
                                                                              TTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCG                 <  SRR4302545.5573610/94‑1 (MQ=60)
                                                                               TGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGG                >  SRR4302545.4525869/1‑94 (MQ=60)
                                                                                GTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGG               <  SRR4302545.3889121/94‑1 (MQ=60)
                                                                                GTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGG               <  SRR4302545.6224408/94‑1 (MQ=60)
                                                                                GTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGG               >  SRR4302545.7675105/1‑94 (MQ=60)
                                                                                 TCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGA              >  SRR4302545.2635339/1‑94 (MQ=60)
                                                                                 TCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGA              <  SRR4302545.5254134/94‑1 (MQ=60)
                                                                                 TCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGA              <  SRR4302545.8732529/94‑1 (MQ=60)
                                                                                  CGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAG             >  SRR4302545.531841/1‑94 (MQ=60)
                                                                                  CGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAG             <  SRR4302545.261867/94‑1 (MQ=60)
                                                                                  CGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAG             >  SRR4302545.8324334/1‑94 (MQ=60)
                                                                                   GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTGTCATCCACGCGTATTGCGGGAGG            >  SRR4302545.7802109/1‑94 (MQ=60)
                                                                                   GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGG            <  SRR4302545.3278434/94‑1 (MQ=60)
                                                                                   GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGG            <  SRR4302545.5813299/94‑1 (MQ=60)
                                                                                   GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGG            <  SRR4302545.2097101/94‑1 (MQ=60)
                                                                                     TACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCG          >  SRR4302545.9104012/1‑94 (MQ=60)
                                                                                     TACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCA          >  SRR4302545.1344546/1‑94 (MQ=60)
                                                                                      ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         <  SRR4302545.3259203/94‑1 (MQ=60)
                                                                                      ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         <  SRR4302545.10925899/94‑1 (MQ=60)
                                                                                      ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         >  SRR4302545.7365942/1‑94 (MQ=60)
                                                                                      ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         <  SRR4302545.2726667/94‑1 (MQ=60)
                                                                                       CCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGG        >  SRR4302545.1981626/1‑94 (MQ=60)
                                                                                        CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGT       <  SRR4302545.1353447/94‑1 (MQ=60)
                                                                                        CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGT       >  SRR4302545.1690424/1‑94 (MQ=60)
                                                                                        CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGT       <  SRR4302545.9864048/94‑1 (MQ=60)
                                                                                        CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGG       >  SRR4302545.5304417/1‑94 (MQ=60)
                                                                                        CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGG       >  SRR4302545.7763644/1‑94 (MQ=60)
                                                                                         AACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG      <  SRR4302545.7492519/94‑1 (MQ=60)
                                                                                         AACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG      >  SRR4302545.2120391/1‑94 (MQ=60)
                                                                                         AACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG      <  SRR4302545.2383797/94‑1 (MQ=60)
                                                                                          ACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGC     <  SRR4302545.1812973/94‑1 (MQ=60)
                                                                                           CGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGtaggtttt    >  SRR4302545.9954351/1‑86 (MQ=60)
                                                                                           CGATGAGCGTATGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCC    >  SRR4302545.7436547/1‑94 (MQ=60)
                                                                                            GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCTGGAGGCAGGagcag   >  SRR4302545.1284961/1‑89 (MQ=60)
                                                                                            GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCG   >  SRR4302545.612467/1‑94 (MQ=60)
                                                                                            GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCG   >  SRR4302545.7392979/1‑94 (MQ=60)
                                                                                            GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACACGTATTGCGGGAGGCAGGTGCCG   >  SRR4302545.6050906/1‑94 (MQ=60)
                                                                                            GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTAATCCACGCGTATTGCGGGAGGCAGGTGCCG   >  SRR4302545.4278408/1‑94 (MQ=60)
                                                                                             ATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  SRR4302545.901142/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  NC_000913/3434159‑3434345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑