Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,252 G→A G33D (GGT→GAT)  trkA → TrkA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,2520GA100.0% 838.0 / NA 233G33D (GGT→GAT) trkATrkA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (125/108);  total (125/108)

Only 100 of 236 total aligned reads displayed.

TTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG  >  NC_000913/3434162‑3434341
                                                                                          |                                                                                         
ttATCATTCTGGGTGCCGGCCAGGTTGGCTGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATg                                                                                         <  2:3995718/93‑1 (MQ=255)
ttATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATg                                                                                         <  2:5139461/93‑1 (MQ=255)
 tATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGa                                                                                        <  2:3531273/93‑1 (MQ=255)
   tCNTTCNGGCTGCCGGCCAGGNNGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGc                                                                                      <  1:3015427/93‑1 (MQ=255)
   tCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGc                                                                                      <  2:5649661/93‑1 (MQ=255)
     aTTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGt                                                                                    <  2:1644171/93‑1 (MQ=255)
      ttCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTc                                                                                   >  2:1416816/1‑93 (MQ=255)
      ttCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTc                                                                                   >  2:1845450/1‑93 (MQ=255)
      ttCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGGTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGtt                                                                                   >  2:3844672/1‑92 (MQ=255)
       tCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCt                                                                                  >  1:5038773/1‑93 (MQ=255)
         tGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                <  1:2215425/93‑1 (MQ=255)
         tGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                >  1:4858575/1‑93 (MQ=255)
         tGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                >  1:861957/1‑93 (MQ=255)
         tGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                >  2:4740705/1‑93 (MQ=255)
            gTGCCGGCCAGGCTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGa                                                                             >  2:6430898/1‑93 (MQ=255)
                cGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCtt                                                                         <  2:2180109/93‑1 (MQ=255)
                 ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTa                                                                        <  2:1807238/93‑1 (MQ=255)
                  gCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTAc                                                                       >  2:2719002/1‑93 (MQ=255)
                     aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAgg                                                                    <  2:2104803/93‑1 (MQ=255)
                      ggTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGa                                                                   <  2:793352/93‑1 (MQ=255)
                      ggTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGa                                                                   >  2:257635/1‑93 (MQ=255)
                      ggTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGa                                                                   >  1:5582407/1‑93 (MQ=255)
                      ggTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGa                                                                   >  1:2754570/1‑93 (MQ=255)
                       gTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGAt                                                                  >  1:2774311/1‑93 (MQ=255)
                        ttGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTATTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATa                                                                 <  1:4552061/93‑1 (MQ=255)
                           gcggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAAt                                                              <  1:11162/93‑1 (MQ=255)
                           gcggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAAt                                                              <  2:5873049/93‑1 (MQ=255)
                               cacaCTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGa                                                          <  2:4624932/93‑1 (MQ=255)
                                acacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAc                                                         >  1:3432115/1‑93 (MQ=255)
                                acacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAc                                                         <  1:4992321/93‑1 (MQ=255)
                                acacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAc                                                         <  1:6231981/93‑1 (MQ=255)
                                 cacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAcc                                                        <  1:4102639/93‑1 (MQ=255)
                                 cacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAcc                                                        <  2:3345847/93‑1 (MQ=255)
                                   cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTg                                                      <  1:4808721/93‑1 (MQ=255)
                                    tGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGc                                                     <  1:1151725/93‑1 (MQ=255)
                                    tGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGc                                                     <  1:935749/93‑1 (MQ=255)
                                       cGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCggg                                                  >  1:5455603/1‑93 (MQ=255)
                                         gAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTc                                                >  1:4650408/1‑93 (MQ=255)
                                         gAAAACCTGGTTAGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTc                                                >  2:6273547/1‑93 (MQ=255)
                                            aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTg                                             <  1:1051044/93‑1 (MQ=255)
                                             aCCTGGTTGGCGAGAACAACGATATTACTGTTGGCGATACCAACGATGACCGTCTGCGGACCTTACAGCATAAATTTTACCTGCAGGCCGAGc                                            >  2:4275735/1‑93 (MQ=255)
                                               cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                          >  2:3735575/1‑93 (MQ=255)
                                               cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                          >  2:1638123/1‑93 (MQ=255)
                                               cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                          >  1:3350548/1‑93 (MQ=255)
                                                tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAgg                                         >  1:3082754/1‑93 (MQ=255)
                                                 ggTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAggg                                        >  2:4986906/1‑93 (MQ=255)
                                                    tGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCa                                     <  1:5385778/93‑1 (MQ=255)
                                                    tGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCa                                     <  2:1481066/93‑1 (MQ=255)
                                                     ggCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAt                                    >  2:378059/1‑93 (MQ=255)
                                                     ggCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAt                                    <  2:5548124/93‑1 (MQ=255)
                                                     ggCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAt                                    <  1:4925791/93‑1 (MQ=255)
                                                     ggCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAt                                    <  2:2889083/93‑1 (MQ=255)
                                                        gagaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGc                                 >  2:263276/1‑93 (MQ=255)
                                                        gagaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGc                                 >  2:2057515/1‑93 (MQ=255)
                                                         agaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGct                                >  1:2643750/1‑93 (MQ=255)
                                                          gaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGctc                               >  2:3662529/1‑93 (MQ=255)
                                                            acaacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCtctc                             >  2:6064405/1‑93 (MQ=255)
                                                            acaacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCtctc                             <  2:2070078/93‑1 (MQ=255)
                                                             caacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCACGGCTCTCa                            <  1:2911585/93‑1 (MQ=255)
                                                             caacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGCGTCGTGCAGGGGCATGGCTCTCa                            >  1:3635103/1‑93 (MQ=255)
                                                              aacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAt                           >  2:584281/1‑93 (MQ=255)
                                                              aacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAt                           >  2:1459963/1‑93 (MQ=255)
                                                              aacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAt                           >  1:2515776/1‑93 (MQ=255)
                                                               acGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATc                          >  2:630181/1‑93 (MQ=255)
                                                               acGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATc                          >  2:3695580/1‑93 (MQ=255)
                                                                cGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATcc                         >  1:3566599/1‑93 (MQ=255)
                                                                cGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATcc                         >  1:5741215/1‑93 (MQ=255)
                                                                 gATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCa                        <  1:2396756/93‑1 (MQ=255)
                                                                 catatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGGCCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCa                        <  2:3016121/92‑1 (MQ=255)
                                                                  atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAc                       <  2:2326526/93‑1 (MQ=255)
                                                                  atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAc                       >  2:3068050/1‑93 (MQ=255)
                                                                  atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAc                       <  1:5649412/93‑1 (MQ=255)
                                                                  atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAc                       <  2:5417031/93‑1 (MQ=255)
                                                                  atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAc                       <  1:1642294/93‑1 (MQ=255)
                                                                   tatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                      >  1:4377900/1‑93 (MQ=255)
                                                                   tatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                      >  1:1741651/1‑93 (MQ=255)
                                                                   tatTACTGTTGTCGATACCAACGATGAGAGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                      >  2:3699320/1‑93 (MQ=255)
                                                                    atTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGGCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcgc                     <  2:2816243/93‑1 (MQ=255)
                                                                     ttACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcgcg                    >  2:2590753/1‑93 (MQ=255)
                                                                     ttACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcgcg                    >  1:4268634/1‑93 (MQ=255)
                                                                        cTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAt                 <  1:5338238/93‑1 (MQ=255)
                                                                        cTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAt                 >  1:1217480/1‑93 (MQ=255)
                                                                         tgttgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAtt                >  1:1407976/1‑93 (MQ=255)
                                                                           ttgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGc              >  2:1206490/1‑93 (MQ=255)
                                                                            tgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCg             >  1:5801503/1‑93 (MQ=255)
                                                                            tgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCg             >  1:205360/1‑93 (MQ=255)
                                                                             gtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCgg            >  1:5121428/1‑93 (MQ=255)
                                                                              tCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCggg           <  2:1861673/93‑1 (MQ=255)
                                                                                gATACCAACGATGAGCGTCTGCGTACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAg         >  2:1263893/1‑93 (MQ=255)
                                                                                gATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAg         <  2:4679830/93‑1 (MQ=255)
                                                                                  tACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAgga       >  1:714186/1‑92 (MQ=255)
                                                                                  tACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGc       >  2:3272579/1‑93 (MQ=255)
                                                                                   aCCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCa      <  1:2106852/93‑1 (MQ=255)
                                                                                   aCCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCa      >  1:575716/1‑93 (MQ=255)
                                                                                     cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAgg    <  2:1996037/93‑1 (MQ=255)
                                                                                     cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAgg    >  2:5058973/1‑93 (MQ=255)
                                                                                      aaCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAggg   >  1:6057385/1‑92 (MQ=255)
                                                                                      aaCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGt   <  1:1892568/93‑1 (MQ=255)
                                                                                       aCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTg  >  2:4454722/1‑93 (MQ=255)
                                                                                       aCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTg  <  1:1947513/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG  >  NC_000913/3434162‑3434341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  NC_000913/3434160‑3434345
                                                                                            |                                                                                             
AATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGAT                                                                                              <  SRR4302537.1685538/94‑1 (MQ=60)
 ATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATG                                                                                             <  SRR4302537.3278550/94‑1 (MQ=60)
  TTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGA                                                                                            >  SRR4302537.2431163/1‑94 (MQ=60)
   TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           >  SRR4302537.136297/1‑94 (MQ=60)
   TATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAG                                                                                           <  SRR4302537.5804913/94‑1 (MQ=60)
     TCNTTCNGGCTGCCGGCCAGGNNGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCG                                                                                         <  SRR4302537.3036450/94‑1 (MQ=60)
        TTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCT                                                                                      >  SRR4302537.5073458/1‑94 (MQ=60)
          CTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGC                                                                                    >  SRR4302537.4892097/1‑94 (MQ=60)
          CTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGC                                                                                    >  SRR4302537.1947584/1‑94 (MQ=60)
          CTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGC                                                                                    >  SRR4302537.867865/1‑94 (MQ=60)
           TGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCG                                                                                   <  SRR4302537.2231047/94‑1 (MQ=60)
                 CCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTT                                                                             <  SRR4302537.1496959/94‑1 (MQ=60)
                   GGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTAC                                                                           <  SRR4302537.582440/94‑1 (MQ=60)
                   GGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTAA                                                                           >  SRR4302537.5783918/1‑94 (MQ=60)
                     CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302537.4199693/94‑1 (MQ=60)
                     CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302537.5645375/94‑1 (MQ=60)
                     CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         <  SRR4302537.2572314/94‑1 (MQ=60)
                     CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         >  SRR4302537.115828/1‑94 (MQ=60)
                     CCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAG                                                                         >  SRR4302537.4941775/1‑94 (MQ=60)
                      CAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGG                                                                        >  SRR4302537.2089507/1‑94 (MQ=60)
                       AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302537.1736477/1‑94 (MQ=60)
                       AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302537.5620568/1‑94 (MQ=60)
                       AGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGA                                                                       >  SRR4302537.2773801/1‑94 (MQ=60)
                        GGTTGGCGGTACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGAT                                                                      >  SRR4302537.4899900/1‑94 (MQ=60)
                        GGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGAT                                                                      >  SRR4302537.2793704/1‑94 (MQ=60)
                         GTTGGCGGCACACTGGCGGAAAATCTGGTTGGCGAGAACCACGCTATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAGCTTACGGGATA                                                                     >  SRR4302537.2275674/1‑94 (MQ=60)
                          TTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTATTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAA                                                                    <  SRR4302537.4583559/94‑1 (MQ=60)
                             GCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATT                                                                 <  SRR4302537.11244/94‑1 (MQ=60)
                               GGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTG                                                               <  SRR4302537.451125/94‑1 (MQ=60)
                                 CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             >  SRR4302537.6378469/1‑94 (MQ=60)
                                 CACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAC                                                             >  SRR4302537.3456407/1‑94 (MQ=60)
                                  ACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACC                                                            <  SRR4302537.5026723/94‑1 (MQ=60)
                                  ACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACC                                                            <  SRR4302537.6274846/94‑1 (MQ=60)
                                  ACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACC                                                            <  SRR4302537.871242/94‑1 (MQ=60)
                                   CACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCT                                                           <  SRR4302537.4131290/94‑1 (MQ=60)
                                     CTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGC                                                         <  SRR4302537.4841911/94‑1 (MQ=60)
                                     CTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGC                                                         <  SRR4302537.3395156/94‑1 (MQ=60)
                                      TGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCG                                                        <  SRR4302537.6264534/94‑1 (MQ=60)
                                      TGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCG                                                        <  SRR4302537.1159734/94‑1 (MQ=60)
                                      TGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCG                                                        <  SRR4302537.942196/94‑1 (MQ=60)
                                       GGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGG                                                       >  SRR4302537.1083596/1‑94 (MQ=60)
                                        GCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGG                                                      >  SRR4302537.5492817/1‑94 (MQ=60)
                                          GGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    >  SRR4302537.5723209/1‑94 (MQ=60)
                                          GGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTC                                                    >  SRR4302537.4682560/1‑94 (MQ=60)
                                            AAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGT                                                  >  SRR4302537.2778433/1‑94 (MQ=60)
                                             AAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTG                                                 >  SRR4302537.2852280/1‑94 (MQ=60)
                                              AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                <  SRR4302537.1058295/94‑1 (MQ=60)
                                              AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                <  SRR4302537.2027141/94‑1 (MQ=60)
                                              AACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGC                                                <  SRR4302537.4680952/94‑1 (MQ=60)
                                               ACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATGAATTTGACTTGCGGGTCGTGCG                                               >  SRR4302537.5134879/1‑94 (MQ=60)
                                                CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              >  SRR4302537.3374271/1‑94 (MQ=60)
                                                CCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAG                                              >  SRR4302537.2187352/1‑94 (MQ=60)
                                                 CTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGG                                             >  SRR4302537.1185125/1‑94 (MQ=60)
                                                 CTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGG                                             >  SRR4302537.3104227/1‑94 (MQ=60)
                                                      TGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAT                                        <  SRR4302537.5640534/94‑1 (MQ=60)
                                                      TGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCAT                                        <  SRR4302537.5422594/94‑1 (MQ=60)
                                                       GGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATG                                       >  SRR4302537.273683/1‑94 (MQ=60)
                                                       GGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATG                                       <  SRR4302537.4959749/94‑1 (MQ=60)
                                                       GGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATG                                       <  SRR4302537.5015455/94‑1 (MQ=60)
                                                        GCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGG                                      <  SRR4302537.2311142/94‑1 (MQ=60)
                                                        GCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGG                                      <  SRR4302537.1751984/94‑1 (MQ=60)
                                                         CGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGC                                     <  SRR4302537.2986677/94‑1 (MQ=60)
                                                          GAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCT                                    >  SRR4302537.5517102/1‑94 (MQ=60)
                                                          GAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCT                                    >  SRR4302537.2662243/1‑94 (MQ=60)
                                                            GAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCT                                  <  SRR4302537.4872809/94‑1 (MQ=60)
                                                            GAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCT                                  <  SRR4302537.2273616/94‑1 (MQ=60)
                                                            GAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCT                                  >  SRR4302537.6506257/1‑94 (MQ=60)
                                                              ACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCA                                >  SRR4302537.5134031/1‑94 (MQ=60)
                                                              ACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCA                                >  SRR4302537.1222619/1‑94 (MQ=60)
                                                              ACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGCGTCGTGCAGGGGCATGGCTCTCA                                >  SRR4302537.3660706/1‑94 (MQ=60)
                                                               CAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAT                               >  SRR4302537.2533454/1‑94 (MQ=60)
                                                               CAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCACGGCTCTCAT                               <  SRR4302537.2932040/94‑1 (MQ=60)
                                                                AACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATC                              <  SRR4302537.3641409/94‑1 (MQ=60)
                                                                AACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATC                              >  SRR4302537.5488756/1‑94 (MQ=60)
                                                                 ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             >  SRR4302537.5780525/1‑94 (MQ=60)
                                                                 ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             >  SRR4302537.5460031/1‑94 (MQ=60)
                                                                 ACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC                             >  SRR4302537.3591750/1‑94 (MQ=60)
                                                                   GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           >  SRR4302537.3185437/1‑94 (MQ=60)
                                                                   GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           <  SRR4302537.2413631/94‑1 (MQ=60)
                                                                   GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           <  SRR4302537.4780343/94‑1 (MQ=60)
                                                                   GATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAC                           <  SRR4302537.4188507/94‑1 (MQ=60)
                                                                    ATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          <  SRR4302537.5688078/94‑1 (MQ=60)
                                                                    ATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          <  SRR4302537.1653797/94‑1 (MQ=60)
                                                                    ATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          >  SRR4302537.1753791/1‑94 (MQ=60)
                                                                    ATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACG                          >  SRR4302537.4408127/1‑94 (MQ=60)
                                                                     TATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGC                         >  SRR4302537.5333076/1‑94 (MQ=60)
                                                                     TATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGC                         <  SRR4302537.2037096/94‑1 (MQ=60)
                                                                      ATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCG                        >  SRR4302537.4298293/1‑94 (MQ=60)
                                                                         ACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAT                     >  SRR4302537.1225946/1‑94 (MQ=60)
                                                                         ACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAT                     >  SRR4302537.3507509/1‑94 (MQ=60)
                                                                          CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    <  SRR4302537.5374834/94‑1 (MQ=60)
                                                                          CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    >  SRR4302537.1417782/1‑94 (MQ=60)
                                                                          CTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATT                    >  SRR4302537.5949083/1‑94 (MQ=60)
                                                                           TGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTG                   <  SRR4302537.541969/94‑1 (MQ=60)
                                                                           TGTTGGCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTG                   <  SRR4302537.2570832/94‑1 (MQ=60)
                                                                             TTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCG                 >  SRR4302537.5841277/1‑94 (MQ=60)
                                                                             TTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCG                 >  SRR4302537.206832/1‑94 (MQ=60)
                                                                              TGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGG                >  SRR4302537.5156727/1‑94 (MQ=60)
                                                                               GTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGG               >  SRR4302537.5072215/1‑94 (MQ=60)
                                                                                  GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGG            <  SRR4302537.3622148/94‑1 (MQ=60)
                                                                                  GATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGG            <  SRR4302537.4677067/94‑1 (MQ=60)
                                                                                   ATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGA           >  SRR4302537.719071/1‑94 (MQ=60)
                                                                                    TACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCA          <  SRR4302537.3846022/94‑1 (MQ=60)
                                                                                    TACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCA          <  SRR4302537.313581/94‑1 (MQ=60)
                                                                                    TACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCA          >  SRR4302537.579662/1‑94 (MQ=60)
                                                                                     ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         >  SRR4302537.80750/1‑94 (MQ=60)
                                                                                     ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         <  SRR4302537.2121703/94‑1 (MQ=60)
                                                                                     ACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAG         <  SRR4302537.2810903/94‑1 (MQ=60)
                                                                                       CACCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGT       <  SRR4302537.5640510/94‑1 (MQ=60)
                                                                                       CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGT       <  SRR4302537.1784854/94‑1 (MQ=60)
                                                                                       CAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGG       >  SRR4302537.6098995/1‑94 (MQ=60)
                                                                                        AACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG      <  SRR4302537.1905722/94‑1 (MQ=60)
                                                                                        AACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTG      <  SRR4302537.3037725/94‑1 (MQ=60)
                                                                                         ACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGC     <  SRR4302537.1961056/94‑1 (MQ=60)
                                                                                         ACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGC     <  SRR4302537.4401665/94‑1 (MQ=60)
                                                                                           GATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCG   >  SRR4302537.1614760/1‑94 (MQ=60)
                                                                                            ATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  SRR4302537.5023728/1‑94 (MQ=60)
                                                                                            ATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  <  SRR4302537.4993508/94‑1 (MQ=60)
                                                                                            ATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  SRR4302537.3274212/1‑94 (MQ=60)
                                                                                            |                                                                                             
AATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGA  >  NC_000913/3434160‑3434345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑