Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,434,252 G→A G33D (GGT→GAT)  trkA → TrkA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,434,2520GA99.0% 1896.8 / 0.4 495G33D (GGT→GAT) trkATrkA
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base A (229/261);  total (233/262)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

Only 100 of 503 total aligned reads displayed.

ATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGC  >  NC_000913/3434161‑3434342
                                                                                           |                                                                                          
aTTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATg                                                                                          <  2:4981501/94‑1 (MQ=255)
 ttATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGa                                                                                         <  1:10068426/94‑1 (MQ=255)
 ttATCATTCTGGGTGCCGACCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGa                                                                                         >  1:9265552/1‑94 (MQ=255)
  tATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAg                                                                                        >  2:2303274/1‑94 (MQ=255)
   aTCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGc                                                                                       <  2:1172615/94‑1 (MQ=255)
     cATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGt                                                                                     <  2:3749479/94‑1 (MQ=255)
      aTTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTc                                                                                    <  1:1177142/94‑1 (MQ=255)
       ttCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCt                                                                                   >  2:7431884/1‑94 (MQ=255)
         cTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                 >  1:5490092/1‑94 (MQ=255)
         cTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGc                                                                                 <  2:4574971/94‑1 (MQ=255)
          tGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCg                                                                                >  2:1850309/1‑94 (MQ=255)
           gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCgg                                                                               <  2:8079691/94‑1 (MQ=255)
           gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCgg                                                                               <  1:846149/94‑1 (MQ=255)
            ggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGa                                                                              <  2:4219032/94‑1 (MQ=255)
              tgcctgccAGGTTGGCGGCGCACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAcc                                                                            <  1:230385/94‑1 (MQ=255)
              tGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAcc                                                                            >  2:6640976/1‑94 (MQ=255)
              tGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAcc                                                                            >  1:9550660/1‑94 (MQ=255)
              tGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGAcc                                                                            >  1:8238152/1‑94 (MQ=255)
               gCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTTGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCt                                                                           >  1:6688864/1‑94 (MQ=255)
                 cGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTa                                                                         <  2:9792262/94‑1 (MQ=255)
                 cGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACCATGAGCGTCTGCGGGCCTTa                                                                         >  1:1990034/1‑94 (MQ=255)
                  ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTAc                                                                        <  2:1373532/94‑1 (MQ=255)
                    gcAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAg                                                                      <  2:6710586/93‑1 (MQ=255)
                    ccAGGTTGGCGGCACACTGGCGGAAATCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAg                                                                      <  1:7626903/94‑1 (MQ=255)
                    ccAGGTTGGCGGCACACTGGCGGAAACCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAg                                                                      <  1:10450249/94‑1 (MQ=255)
                     cAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAgg                                                                     >  2:9462469/1‑94 (MQ=255)
                     cAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAgg                                                                     >  2:8953285/1‑94 (MQ=255)
                        gTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATa                                                                  <  2:2198581/94‑1 (MQ=255)
                            gcggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAAtt                                                              <  2:2997119/94‑1 (MQ=255)
                              ggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTg                                                            <  2:1616994/94‑1 (MQ=255)
                               gcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTgc                                                           >  2:3602376/1‑93 (MQ=255)
                                cacaCTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAc                                                          >  2:1247756/1‑94 (MQ=255)
                                cacaCTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGAc                                                          <  1:2810062/94‑1 (MQ=255)
                                   acTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTg                                                       <  1:3155839/94‑1 (MQ=255)
                                   acTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTg                                                       <  1:3976733/94‑1 (MQ=255)
                                    cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGc                                                      <  1:7303788/94‑1 (MQ=255)
                                     tGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCg                                                     <  1:3518045/94‑1 (MQ=255)
                                     tGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCg                                                     >  1:2459815/1‑94 (MQ=255)
                                         ggAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTc                                                 <  1:3427310/94‑1 (MQ=255)
                                         ggAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTc                                                 >  1:2572641/1‑94 (MQ=255)
                                          gAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCg                                                <  1:4354918/94‑1 (MQ=255)
                                           aaaaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGt                                               >  2:4084037/1‑94 (MQ=255)
                                             aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGc                                             >  2:6446875/1‑94 (MQ=255)
                                             aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGc                                             <  1:1664534/94‑1 (MQ=255)
                                             aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGc                                             >  1:5235380/1‑94 (MQ=255)
                                             aaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGc                                             >  1:4835051/1‑94 (MQ=255)
                                              aCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCa                                            <  1:4529837/94‑1 (MQ=255)
                                               ccTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                           <  1:8536831/94‑1 (MQ=255)
                                               ccTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                           >  2:4705286/1‑94 (MQ=255)
                                               ccTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAg                                           <  2:7870920/94‑1 (MQ=255)
                                                cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAgg                                          >  1:7970318/1‑94 (MQ=255)
                                                cTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAgg                                          <  1:2935145/94‑1 (MQ=255)
                                                 tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAggg                                         <  1:5020458/94‑1 (MQ=255)
                                                 tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAggg                                         >  1:1781447/1‑94 (MQ=255)
                                                   gTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGc                                       <  2:616469/94‑1 (MQ=255)
                                                      ggCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATg                                    <  1:2141225/94‑1 (MQ=255)
                                                       gCGAGAACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATgg                                   <  2:3442240/94‑1 (MQ=255)
                                                         gagaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGct                                 <  2:6312228/94‑1 (MQ=255)
                                                           gaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCTGGTCGTGCAGGGGCATGGctct                               <  2:2456318/94‑1 (MQ=255)
                                                           gaACAACGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGctct                               <  2:8269743/94‑1 (MQ=255)
                                                            aacaacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCtctc                              >  2:6939019/1‑94 (MQ=255)
                                                             acaacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCa                             <  2:702549/94‑1 (MQ=255)
                                                             acaacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCa                             <  1:8887633/94‑1 (MQ=255)
                                                              caacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCAt                            >  2:2680819/1‑94 (MQ=255)
                                                               aacGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATc                           >  2:10210704/1‑94 (MQ=255)
                                                                acGATATTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATcc                          <  1:7352107/94‑1 (MQ=255)
                                                                   atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                       >  1:9819887/1‑94 (MQ=255)
                                                                   atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                       <  2:9110113/94‑1 (MQ=255)
                                                                   atatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                       <  2:3231529/94‑1 (MQ=255)
                                                                    tatTACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcgc                      >  1:10606539/1‑94 (MQ=255)
                                                                     atTACTGTTGTCGATACCAACTATGAGCGTCTGCGGCCCTTACAGGATAAATTTGACCTGCTGGTCGTGCAGGGGCATGGCTCTCATTCActct                     >  1:9642811/1‑91 (MQ=255)
                                                                      ttACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGt                    >  1:2697442/1‑94 (MQ=255)
                                                                      ttACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGt                    >  2:5397205/1‑94 (MQ=255)
                                                                       tACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGTGGCATGGCTCTCATCCACGCGTa                   >  1:5830690/1‑94 (MQ=255)
                                                                       tACTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTa                   >  1:6064870/1‑94 (MQ=255)
                                                                         cTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAtt                 >  1:4660466/1‑94 (MQ=255)
                                                                         cTGTTGTCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAtt                 >  1:629585/1‑94 (MQ=255)
                                                                          tgttgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCTTCCACGCGTATTg                >  1:7067142/1‑94 (MQ=255)
                                                                            ttgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCg              <  2:7254549/94‑1 (MQ=255)
                                                                            ttgtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCg              >  2:10363333/1‑94 (MQ=255)
                                                                              gtCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCggg            <  1:3795068/94‑1 (MQ=255)
                                                                               tCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGa           <  2:8345614/94‑1 (MQ=255)
                                                                               tCGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGa           <  2:7641396/94‑1 (MQ=255)
                                                                                cGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAg          >  1:520212/1‑94 (MQ=255)
                                                                                cGATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAg          <  2:10542843/94‑1 (MQ=255)
                                                                                 gATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAgg         <  2:8527043/94‑1 (MQ=255)
                                                                                 gATACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAgg         <  2:2825373/94‑1 (MQ=255)
                                                                                  aTACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGc        <  2:5965566/94‑1 (MQ=255)
                                                                                   tACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCCCGCGTATTGCGGGAGGcg       >  2:8715179/1‑93 (MQ=255)
                                                                                   tACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCa       <  2:7549918/94‑1 (MQ=255)
                                                                                   tACCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCa       <  2:5772819/94‑1 (MQ=255)
                                                                                    aCCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAg      <  2:559647/94‑1 (MQ=255)
                                                                                    aCCAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAg      >  1:7166899/1‑94 (MQ=255)
                                                                                     ccAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAgg     <  2:3067901/94‑1 (MQ=255)
                                                                                      cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAggg    >  1:7550191/1‑93 (MQ=255)
                                                                                      cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAggg    >  1:3276084/1‑93 (MQ=255)
                                                                                      cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGt    <  1:1324011/94‑1 (MQ=255)
                                                                                      cAACGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGt    <  2:5143707/94‑1 (MQ=255)
                                                                                       aaCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTg   >  2:7121168/1‑94 (MQ=255)
                                                                                       aaCGATGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTg   >  1:2069441/1‑94 (MQ=255)
                                                                                           |                                                                                          
ATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGC  >  NC_000913/3434161‑3434342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 415 total aligned reads displayed.
CCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCT  >  NC_000913/3438627‑3438813
                                                                                             |                                                                                             
CCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGC                                                                                               <  SRR4302545.6206335/94‑1 (MQ=60)
CCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGC                                                                                               >  SRR4302545.2805689/1‑94 (MQ=60)
CCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGC                                                                                               <  SRR4302545.9438888/94‑1 (MQ=60)
 CAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCG                                                                                              >  SRR4302545.2220153/1‑94 (MQ=60)
 CAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCG                                                                                              <  SRR4302545.1706907/94‑1 (MQ=60)
 CAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCG                                                                                              >  SRR4302545.1609642/1‑94 (MQ=60)
 CAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCG                                                                                              >  SRR4302545.1504042/1‑94 (MQ=60)
 CAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCG                                                                                              >  SRR4302545.5221974/1‑94 (MQ=60)
  CGGCGTGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGT                                                                                             <  SRR4302545.8720203/94‑1 (MQ=60)
  AGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGT                                                                                             >  SRR4302545.9472605/1‑94 (MQ=60)
   GGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTT                                                                                            >  SRR4302545.9220155/1‑94 (MQ=60)
   GGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTT                                                                                            <  SRR4302545.10067777/94‑1 (MQ=60)
   GGCGCCGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTT                                                                                            <  SRR4302545.3070000/94‑1 (MQ=60)
    GCGCGGTTTTAGAAACTCTGTCACAGCACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTC                                                                                           <  SRR4302545.2868073/94‑1 (MQ=60)
    GCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTC                                                                                           <  SRR4302545.10905771/94‑1 (MQ=60)
    GCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTC                                                                                           <  SRR4302545.5619109/94‑1 (MQ=60)
    GCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTC                                                                                           <  SRR4302545.2312256/94‑1 (MQ=60)
      GCTGTTTTAGAATCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGT                                                                                         <  SRR4302545.4382567/94‑1 (MQ=60)
      GCGGTTTTAGATACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGT                                                                                         <  SRR4302545.7363546/94‑1 (MQ=60)
      GCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGT                                                                                         <  SRR4302545.2592935/94‑1 (MQ=60)
       CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTT                                                                                        >  SRR4302545.1169511/1‑94 (MQ=60)
       CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTT                                                                                        >  SRR4302545.10044969/1‑94 (MQ=60)
       CGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTT                                                                                        >  SRR4302545.9714630/1‑94 (MQ=60)
        TGTTTTAGAAACGCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTA                                                                                       <  SRR4302545.7188862/94‑1 (MQ=60)
         GTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAA                                                                                      <  SRR4302545.8519619/94‑1 (MQ=60)
         GTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAA                                                                                      <  SRR4302545.8524234/94‑1 (MQ=60)
         GTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAA                                                                                      >  SRR4302545.903996/1‑94 (MQ=60)
          TTTTAGAAGCTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACGAAGCTTTGCTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAAT                                                                                     <  SRR4302545.8491855/94‑1 (MQ=60)
          TTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAAT                                                                                     <  SRR4302545.2621243/94‑1 (MQ=60)
          TTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAAT                                                                                     <  SRR4302545.5277272/94‑1 (MQ=60)
          TTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAAT                                                                                     <  SRR4302545.3186197/94‑1 (MQ=60)
           TTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATG                                                                                    <  SRR4302545.10432843/94‑1 (MQ=60)
            tgatAAACTCGGTCACAGAACCTTGCTGTGTGTCCTCTCTTTGGTACTAAGCCTTACTTGTAGTAAAGCTCGACGATCTGGTGTTCGcgaaagc                                                                                   >  SRR4302545.9697521/5‑87 (MQ=57)
             TAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTC                                                                                  <  SRR4302545.5879780/94‑1 (MQ=60)
             TAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTC                                                                                  >  SRR4302545.6368945/1‑94 (MQ=60)
              AGATACTCGGGCACAGAACCCTGCATTGTTTCCTCTCTTTGGTACTAAGCTTTACTGGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCC                                                                                 <  SRR4302545.4506004/94‑1 (MQ=60)
              AGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCC                                                                                 >  SRR4302545.6003790/1‑94 (MQ=60)
               GAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCG                                                                                >  SRR4302545.4834690/1‑94 (MQ=60)
               GAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCG                                                                                <  SRR4302545.6504812/94‑1 (MQ=60)
                AAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGC                                                                               >  SRR4302545.3720401/1‑94 (MQ=60)
                 AACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCA                                                                              <  SRR4302545.9544777/94‑1 (MQ=60)
                  ACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAG                                                                             <  SRR4302545.6866516/94‑1 (MQ=60)
                    TCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGAC                                                                           >  SRR4302545.10104070/1‑94 (MQ=60)
                     CTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACA                                                                          <  SRR4302545.6791701/94‑1 (MQ=60)
                      TGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAG                                                                         <  SRR4302545.10327023/94‑1 (MQ=60)
                       GTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGA                                                                        >  SRR4302545.8063775/1‑94 (MQ=60)
                       GTCACAGAACCCTGCATTGTGTCCTCTCTTTGGGACGAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGGCCGCAGACAGA                                                                        <  SRR4302545.10483640/94‑1 (MQ=60)
                        TCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGAT                                                                       >  SRR4302545.1898045/1‑94 (MQ=60)
                        TCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGAT                                                                       <  SRR4302545.9259410/94‑1 (MQ=60)
                         CACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATC                                                                      >  SRR4302545.3960011/1‑94 (MQ=60)
                         CACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATC                                                                      <  SRR4302545.8236199/94‑1 (MQ=60)
                         CACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATC                                                                      >  SRR4302545.5017043/1‑94 (MQ=60)
                         CACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATC                                                                      >  SRR4302545.265086/1‑94 (MQ=60)
                         CACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATC                                                                      >  SRR4302545.5252756/1‑94 (MQ=60)
                          ACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCA                                                                     <  SRR4302545.10907440/94‑1 (MQ=60)
                          ACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCA                                                                     <  SRR4302545.8889733/94‑1 (MQ=60)
                          ACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCA                                                                     <  SRR4302545.2109517/94‑1 (MQ=60)
                          ACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGAGTTCGTTAATGTCCGCAGACAGATCA                                                                     >  SRR4302545.6136636/1‑94 (MQ=60)
                           CAGAACCCTGCATTGTGTCCTCTGTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAG                                                                    <  SRR4302545.5969758/94‑1 (MQ=60)
                            AGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGA                                                                   >  SRR4302545.9019960/1‑94 (MQ=60)
                            AGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGA                                                                   >  SRR4302545.5778458/1‑94 (MQ=60)
                            AGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGA                                                                   <  SRR4302545.9374336/94‑1 (MQ=60)
                            AGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGA                                                                   >  SRR4302545.4779158/1‑94 (MQ=60)
                             GAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAA                                                                  <  SRR4302545.8041644/94‑1 (MQ=60)
                             GAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAA                                                                  <  SRR4302545.4205865/94‑1 (MQ=60)
                             GAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAA                                                                  <  SRR4302545.3590024/94‑1 (MQ=60)
                             GAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCACGCGTTCGTTAATGTCCGCAGACAGATCAGAA                                                                  >  SRR4302545.10239068/1‑94 (MQ=60)
                             AAACCCTGCCGTGTGTCCTCTCTTTGGTACTAAGCTTTAAATTGAGTAAAACTCGCCGATCAGGCGTTCCTTAATGTCCGCAGACAGATCAGAA                                                                  <  SRR4302545.9309711/94‑1 (MQ=50)
                              AACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAAC                                                                 <  SRR4302545.5823264/94‑1 (MQ=60)
                              AACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAAC                                                                 <  SRR4302545.917294/94‑1 (MQ=60)
                               ACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACG                                                                >  SRR4302545.4573119/1‑94 (MQ=60)
                                 CCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCT                                                              >  SRR4302545.1801136/1‑94 (MQ=60)
                                  CTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCAGCAGACAGATCAGCACGCTC                                                             >  SRR4302545.5378741/1‑94 (MQ=60)
                                   TGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCC                                                            >  SRR4302545.676499/1‑94 (MQ=60)
                                   TGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCC                                                            <  SRR4302545.670110/94‑1 (MQ=60)
                                   TGCATTGTGTCCTCTCTGTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCC                                                            <  SRR4302545.931476/94‑1 (MQ=60)
                                    GCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCG                                                           <  SRR4302545.8127757/94‑1 (MQ=60)
                                    GCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCG                                                           <  SRR4302545.6643947/94‑1 (MQ=60)
                                    GCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCG                                                           <  SRR4302545.1075249/94‑1 (MQ=60)
                                    GCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCG                                                           <  SRR4302545.1204548/94‑1 (MQ=60)
                                     CATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGG                                                          >  SRR4302545.10931392/1‑94 (MQ=60)
                                      ATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGC                                                         <  SRR4302545.7512468/94‑1 (MQ=60)
                                       TTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGTCAGATCAGAACGCTCCGGCT                                                        <  SRR4302545.2217871/94‑1 (MQ=60)
                                       TTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCT                                                        >  SRR4302545.4756343/1‑94 (MQ=60)
                                        TGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTT                                                       >  SRR4302545.9809036/1‑94 (MQ=60)
                                         GTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTA                                                      <  SRR4302545.8567184/94‑1 (MQ=60)
                                           GTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACG                                                    >  SRR4302545.2422928/1‑94 (MQ=60)
                                           GTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACG                                                    <  SRR4302545.7707175/94‑1 (MQ=60)
                                           GTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACG                                                    >  SRR4302545.2811693/1‑94 (MQ=60)
                                           GTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACG                                                    >  SRR4302545.10141151/1‑94 (MQ=60)
                                            TCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGC                                                   >  SRR4302545.5584125/1‑94 (MQ=60)
                                              CTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTT                                                 <  SRR4302545.2741211/94‑1 (MQ=60)
                                              CTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTT                                                 <  SRR4302545.4897658/94‑1 (MQ=60)
                                              CTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTT                                                 >  SRR4302545.10562548/1‑94 (MQ=60)
                                              CTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTT                                                 <  SRR4302545.5907924/94‑1 (MQ=60)
                                               TCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTA                                                >  SRR4302545.638451/1‑94 (MQ=60)
                                               TCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTA                                                >  SRR4302545.8822309/1‑94 (MQ=60)
                                               TCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTA                                                >  SRR4302545.4915505/1‑94 (MQ=60)
                                               TCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTA                                                <  SRR4302545.4062731/94‑1 (MQ=60)
                                                CTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAA                                               <  SRR4302545.10459030/94‑1 (MQ=60)
                                                CTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAA                                               >  SRR4302545.1393194/1‑94 (MQ=60)
                                                 TCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAA                                              >  SRR4302545.6260023/1‑94 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             <  SRR4302545.1614615/94‑1 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             >  SRR4302545.10193045/1‑94 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             >  SRR4302545.2072003/1‑94 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             >  SRR4302545.6609537/1‑94 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             >  SRR4302545.7583835/1‑94 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             <  SRR4302545.3335526/94‑1 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             <  SRR4302545.8633770/94‑1 (MQ=60)
                                                  CTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAAC                                             <  SRR4302545.8298098/94‑1 (MQ=60)
                                                   TTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACG                                            <  SRR4302545.10360625/94‑1 (MQ=60)
                                                   TTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACG                                            >  SRR4302545.3423941/1‑94 (MQ=60)
                                                    TTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGT                                           >  SRR4302545.907676/1‑94 (MQ=60)
                                                     TGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTA                                          <  SRR4302545.4670903/94‑1 (MQ=60)
                                                      GGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGTTCCGACTCACGCTTAAACGTAC                                         >  SRR4302545.9113919/1‑94 (MQ=60)
                                                      GGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTAC                                         >  SRR4302545.1599926/1‑94 (MQ=60)
                                                       GTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACC                                        >  SRR4302545.9568044/1‑94 (MQ=60)
                                                       GTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACC                                        >  SRR4302545.7951160/1‑94 (MQ=60)
                                                       GTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACC                                        <  SRR4302545.1551024/94‑1 (MQ=60)
                                                        TACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCT                                       >  SRR4302545.4937495/1‑94 (MQ=60)
                                                         ACTAAGCTTTTCTTGGAGCAACGCTCGACGATCAGGCGTTCTTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTT                                      <  SRR4302545.4257807/94‑1 (MQ=60)
                                                         ACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCTGGCTTACGCTTAAACGTACCTT                                      >  SRR4302545.7608237/1‑94 (MQ=60)
                                                         ACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTT                                      <  SRR4302545.6930263/94‑1 (MQ=60)
                                                         ACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTT                                      >  SRR4302545.9828887/1‑94 (MQ=60)
                                                         ACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTT                                      >  SRR4302545.5632549/1‑94 (MQ=60)
                                                          CTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTC                                     >  SRR4302545.6590389/1‑94 (MQ=60)
                                                          CTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTC                                     >  SRR4302545.7646861/1‑94 (MQ=60)
                                                           aaagcaatttatcGAGTAAAGCTAGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCC                                    <  SRR4302545.1850970/81‑1 (MQ=60)
                                                            AAGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCA                                   <  SRR4302545.10358861/94‑1 (MQ=60)
                                                             AGCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCAT                                  >  SRR4302545.5043543/1‑94 (MQ=60)
                                                              GCTTTGCTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATC                                 <  SRR4302545.9052017/94‑1 (MQ=60)
                                                              GCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATC                                 >  SRR4302545.10566018/1‑94 (MQ=60)
                                                              GCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATC                                 <  SRR4302545.4589605/94‑1 (MQ=60)
                                                              GCTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATC                                 >  SRR4302545.1154707/1‑94 (MQ=60)
                                                               CTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCT                                <  SRR4302545.10290742/94‑1 (MQ=60)
                                                               CTTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCT                                <  SRR4302545.4846636/94‑1 (MQ=60)
                                                                TTTACTTGGGGTAAAGCGCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTT                               <  SRR4302545.8216154/94‑1 (MQ=60)
                                                                TTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTT                               >  SRR4302545.6955268/1‑94 (MQ=60)
                                                                TTTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTT                               <  SRR4302545.3513103/94‑1 (MQ=60)
                                                                 TTACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTG                              >  SRR4302545.4439051/1‑94 (MQ=60)
                                                                   ACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTATGCTTAAACGTACCTTCCATCTTGCC                            >  SRR4302545.5910740/1‑94 (MQ=60)
                                                                   ACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCC                            <  SRR4302545.3556026/94‑1 (MQ=60)
                                                                   ACTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCC                            >  SRR4302545.4365361/1‑94 (MQ=60)
                                                                    CTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCA                           >  SRR4302545.7318007/1‑94 (MQ=60)
                                                                    CTTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCACACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCA                           <  SRR4302545.8426277/94‑1 (MQ=60)
                                                                     TTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAG                          <  SRR4302545.10668462/94‑1 (MQ=60)
                                                                     TTGGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAG                          <  SRR4302545.9391838/94‑1 (MQ=60)
                                                                       GGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCA                        <  SRR4302545.9190260/94‑1 (MQ=60)
                                                                       GGAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCA                        >  SRR4302545.7483359/1‑94 (MQ=60)
                                                                        GAGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCAT                       <  SRR4302545.3630285/94‑1 (MQ=60)
                                                                         AGTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATC                      >  SRR4302545.2241069/1‑94 (MQ=60)
                                                                          GTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCA                     <  SRR4302545.2963487/94‑1 (MQ=60)
                                                                          GTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCA                     >  SRR4302545.2899376/1‑94 (MQ=60)
                                                                          GTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCA                     >  SRR4302545.8471226/1‑94 (MQ=60)
                                                                          GTAAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCA                     >  SRR4302545.6104969/1‑94 (MQ=60)
                                                                            AAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAAC                   >  SRR4302545.4002775/1‑94 (MQ=60)
                                                                            AAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAAC                   <  SRR4302545.3774945/94‑1 (MQ=60)
                                                                            AAAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAAC                   >  SRR4302545.5546565/1‑94 (MQ=60)
                                                                             AAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACT                  <  SRR4302545.9669668/94‑1 (MQ=60)
                                                                             AAGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACT                  >  SRR4302545.8752751/1‑94 (MQ=60)
                                                                             AAGCTCGACGACCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACT                  <  SRR4302545.7733741/94‑1 (MQ=60)
                                                                              AGCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTT                 >  SRR4302545.5329556/1‑94 (MQ=60)
                                                                               GCTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTC                >  SRR4302545.2450291/1‑94 (MQ=60)
                                                                                CTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGagatcagagatcgga               <  SRR4302545.1987747/94‑16 (MQ=60)
                                                                                CTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCACCTTCC               >  SRR4302545.408898/1‑94 (MQ=60)
                                                                                CTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCC               <  SRR4302545.7171395/94‑1 (MQ=60)
                                                                                CTCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCC               >  SRR4302545.1795878/1‑94 (MQ=60)
                                                                                CGCGACGATCAGGTCTTCGTTAATGCCCGCAGACAGAGCAGAACGCTCCGGCTTACGCTGAAACGTACCTGCCAACTTGCCAGCATCAACTTCC               <  SRR4302545.5757200/94‑1 (MQ=60)
                                                                                 TCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCA              >  SRR4302545.7802287/1‑94 (MQ=60)
                                                                                 TCGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCA              >  SRR4302545.653588/1‑94 (MQ=60)
                                                                                  CGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAG             <  SRR4302545.3291225/94‑1 (MQ=60)
                                                                                  CGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAG             >  SRR4302545.2178255/1‑94 (MQ=60)
                                                                                  CGACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCGTCCATCTTGCCAGCATCAACTTCCAG             <  SRR4302545.10689990/94‑1 (MQ=60)
                                                                                   GACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGC            >  SRR4302545.7043033/1‑94 (MQ=60)
                                                                                   GACGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGC            >  SRR4302545.7020013/1‑94 (MQ=60)
                                                                                    ACGATCAGGCGTTCGTTAATGTCCGCAGCCAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCACCTTCCAGCC           >  SRR4302545.1554275/1‑94 (MQ=60)
                                                                                     CGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCA          >  SRR4302545.7396898/1‑94 (MQ=60)
                                                                                     CGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCA          >  SRR4302545.6420451/1‑94 (MQ=60)
                                                                                     CGATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCA          >  SRR4302545.2709691/1‑94 (MQ=60)
                                                                                      GATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAG         >  SRR4302545.10451356/1‑94 (MQ=60)
                                                                                      GATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAG         >  SRR4302545.5463911/1‑94 (MQ=60)
                                                                                       ATCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGG        <  SRR4302545.9803110/94‑1 (MQ=60)
                                                                                        TCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGT       <  SRR4302545.2044773/94‑1 (MQ=60)
                                                                                        TCAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGT       >  SRR4302545.6459496/1‑94 (MQ=60)
                                                                                         CAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTT      >  SRR4302545.4241099/1‑94 (MQ=60)
                                                                                         CAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTT      >  SRR4302545.6094020/1‑94 (MQ=60)
                                                                                         CAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTT      >  SRR4302545.8865961/1‑94 (MQ=60)
                                                                                         CAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTT      <  SRR4302545.8717374/94‑1 (MQ=60)
                                                                                         CAGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGATTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGGT      >  SRR4302545.5713814/1‑94 (MQ=60)
                                                                                          AGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTG     >  SRR4302545.5435122/1‑94 (MQ=60)
                                                                                          AGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTG     <  SRR4302545.7091935/94‑1 (MQ=60)
                                                                                          AGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTG     >  SRR4302545.5886888/1‑94 (MQ=60)
                                                                                          AGGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTG     >  SRR4302545.8741780/1‑94 (MQ=60)
                                                                                           GGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGG    <  SRR4302545.6473318/94‑1 (MQ=60)
                                                                                           GGCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGG    <  SRR4302545.10843792/94‑1 (MQ=60)
                                                                                            GCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGgtggg   >  SRR4302545.971066/1‑89 (MQ=60)
                                                                                            GCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGC   >  SRR4302545.4135782/1‑94 (MQ=60)
                                                                                            GCGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGC   >  SRR4302545.5268449/1‑94 (MQ=60)
                                                                                             CGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCT  <  SRR4302545.9147610/94‑1 (MQ=60)
                                                                                             CGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCT  <  SRR4302545.7760232/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGTGTTCGTTAATGTCCGCAGACAGATCAGAACGCTCCGGCTTACGCTTAAACGTACCTTCCATCTTGCCAGCATCAACTTCCAGCCAGGTTGGCT  >  NC_000913/3438627‑3438813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑