Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,448,691 A→G I104T (ATC→ACC)  rplB ← 50S ribosomal subunit protein L2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,6910AG99.2% 2069.3 / ‑0.2 596I104T (ATC→ACC) rplB50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (304/286);  total (306/290)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 619 total aligned reads displayed.

GTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGA  >  NC_000913/3448600‑3448781
                                                                                           |                                                                                          
 ttGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGgtgt                                                                                         <  1:49371/94‑1 (MQ=255)
 ttGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGgtgt                                                                                         <  1:3132903/94‑1 (MQ=255)
   gcgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTaa                                                                                       <  2:821188/94‑1 (MQ=255)
    cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAAc                                                                                      >  2:3579017/1‑94 (MQ=255)
     gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACg                                                                                     <  2:2290652/94‑1 (MQ=255)
      cATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACgg                                                                                    >  2:5032819/1‑94 (MQ=255)
       aTCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGc                                                                                   >  1:88398/1‑94 (MQ=255)
          ggCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGtt                                                                                >  2:3347590/1‑94 (MQ=255)
          ggCAGGGAGGTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGAGTAACGGCGtt                                                                                >  1:5919219/1‑94 (MQ=255)
            cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGAGTAACGGCGTTCa                                                                              >  1:984181/1‑94 (MQ=255)
             aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                             >  2:10111053/1‑94 (MQ=255)
             aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                             >  1:6839604/1‑94 (MQ=255)
             aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                             <  1:6885633/94‑1 (MQ=255)
             aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                             <  2:10412349/94‑1 (MQ=255)
                gtgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGt                                                                          >  1:6062883/1‑94 (MQ=255)
                 tgtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTc                                                                         >  2:882969/1‑94 (MQ=255)
                  gtTACCTGTTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCt                                                                        <  1:1937076/94‑1 (MQ=255)
                  gtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCt                                                                        >  1:4181770/1‑94 (MQ=255)
                  gtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCt                                                                        >  2:9353581/1‑94 (MQ=255)
                  gtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCt                                                                        >  2:9509804/1‑94 (MQ=255)
                   ttACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGGGTAACGGCGTTCACCGTCtt                                                                       >  2:4576030/1‑94 (MQ=255)
                    tACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTTGCCAGCTTTCAGGCCTTTAGGGGCCAGCGCGTAAGGGCGTTCAACGCCttt                                                                      >  1:8656108/1‑94 (MQ=255)
                    tACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCttt                                                                      >  2:7025672/1‑94 (MQ=255)
                      ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGt                                                                    >  2:7869513/1‑94 (MQ=255)
                      ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTCAGGGGCCAGGGTGTAACGGCGTTTACCGTCTTTGt                                                                    >  1:9073738/1‑94 (MQ=255)
                        cggTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCTTCTTTGTAc                                                                  <  1:3770037/93‑1 (MQ=255)
                          gTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAg                                                                <  1:522068/94‑1 (MQ=255)
                          gTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAg                                                                >  2:5551901/1‑94 (MQ=255)
                             tGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAc                                                             <  2:9308970/94‑1 (MQ=255)
                                ttGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAg                                                          <  2:1187370/94‑1 (MQ=255)
                                  gcagcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAgcg                                                        <  1:5101727/94‑1 (MQ=255)
                                  gcagcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAgcg                                                        >  1:148646/1‑94 (MQ=255)
                                   cagcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAgcgc                                                       >  1:10143195/1‑94 (MQ=255)
                                    agcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGcgcg                                                      <  1:4723896/94‑1 (MQ=255)
                                    agcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGcgcg                                                      <  2:3249882/94‑1 (MQ=255)
                                      caTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGAt                                                    <  1:9944596/94‑1 (MQ=255)
                                      caTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGAt                                                    >  1:2567037/1‑94 (MQ=255)
                                       aTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATg                                                   >  1:10010537/1‑94 (MQ=255)
                                         cAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGtt                                                 >  1:342568/1‑94 (MQ=255)
                                          aaCGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTGACCGTCTTTGTACAGAACCAGCGCGATGTTc                                                >  2:1887555/1‑94 (MQ=255)
                                           aCGCCAGACTGAATCTGGTGGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTcg                                               >  2:5403062/1‑94 (MQ=255)
                                             gCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTcgcg                                             <  1:8057127/94‑1 (MQ=255)
                                             gCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTcgcg                                             <  1:649146/94‑1 (MQ=255)
                                              ccAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCgg                                            >  1:1681873/1‑94 (MQ=255)
                                               cAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGa                                           >  1:5405/1‑94 (MQ=255)
                                                aGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGaa                                          >  1:8560280/1‑94 (MQ=255)
                                                aGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGaa                                          >  1:9365141/1‑94 (MQ=255)
                                                 gACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAc                                         <  2:7603539/94‑1 (MQ=255)
                                                 gACTGAATCTGGTCGCCAGCTTTCAGCCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAc                                         <  2:5949589/94‑1 (MQ=255)
                                                  aCTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACg                                        >  1:2935980/1‑94 (MQ=255)
                                                   cTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACgg                                       >  2:1337668/1‑94 (MQ=255)
                                                    tGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAGGGt                                      >  1:2140197/1‑94 (MQ=255)
                                                    tGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGt                                      >  2:3110136/1‑94 (MQ=255)
                                                      aaTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTc                                    <  1:9671495/94‑1 (MQ=255)
                                                      aaTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTc                                    <  1:1123732/94‑1 (MQ=255)
                                                      aaTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTc                                    >  1:4022264/1‑94 (MQ=255)
                                                        tCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCgg                                  >  2:4168630/1‑94 (MQ=255)
                                                          tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGAt                                >  2:2920466/1‑94 (MQ=255)
                                                          tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGAt                                >  1:2222692/1‑94 (MQ=255)
                                                          tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGGGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGAt                                >  2:8712701/1‑94 (MQ=255)
                                                           ggTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATc                               >  2:855090/1‑94 (MQ=255)
                                                           ggTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATc                               <  2:255089/94‑1 (MQ=255)
                                                            gTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCg                              <  1:5864633/94‑1 (MQ=255)
                                                              cGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTa                            >  1:3565082/1‑94 (MQ=255)
                                                              cGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTa                            <  2:5132685/94‑1 (MQ=255)
                                                               gCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTAc                           >  1:8273763/1‑94 (MQ=255)
                                                               gCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTAc                           >  2:6821576/1‑94 (MQ=255)
                                                                ccAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACt                          >  1:9506850/1‑94 (MQ=255)
                                                                ccAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACt                          >  2:6472650/1‑94 (MQ=255)
                                                                ccAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACt                          <  2:3815301/94‑1 (MQ=255)
                                                                ccAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACt                          >  1:431893/1‑94 (MQ=255)
                                                                  aGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCa                        >  1:6155537/1‑94 (MQ=255)
                                                                   gCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCaa                       <  1:9228396/94‑1 (MQ=255)
                                                                   gCTGTCAGGCCTTGAGGGGCCAGGGTGTAGCGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCaa                       <  1:4534351/94‑1 (MQ=255)
                                                                    cTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCaaa                      >  2:9776288/1‑93 (MQ=255)
                                                                      ttCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGAc                    <  1:7352663/94‑1 (MQ=255)
                                                                       tCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACg                   <  1:7973515/94‑1 (MQ=255)
                                                                       tCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACg                   <  1:1294922/94‑1 (MQ=255)
                                                                         aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGtt                 >  2:6169858/1‑94 (MQ=255)
                                                                         aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAACGTCTTTGTACAGAACCACCGCGATGTTCGCGGCACGGTTCGGATCGTACTCAAGACGtt                 >  2:76943/1‑94 (MQ=255)
                                                                          ggCCGTGAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTc                <  1:5990900/94‑1 (MQ=255)
                                                                           gCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTca               >  2:4490332/1‑94 (MQ=255)
                                                                           gCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTca               <  2:6404485/94‑1 (MQ=255)
                                                                           gCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTca               <  2:2729027/94‑1 (MQ=255)
                                                                            ccTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaa              >  2:4849855/1‑94 (MQ=255)
                                                                              tttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaaca            <  1:7533033/94‑1 (MQ=255)
                                                                              tttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaaca            <  2:7519690/94‑1 (MQ=255)
                                                                                tAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCaacaac          <  2:10576643/94‑1 (MQ=255)
                                                                                tAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCaacaac          >  2:7145017/1‑94 (MQ=255)
                                                                                  ggggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTg        <  1:5695200/94‑1 (MQ=255)
                                                                                  ggggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTg        <  2:5319655/94‑1 (MQ=255)
                                                                                  ggggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTg        <  1:8168306/94‑1 (MQ=255)
                                                                                    ggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGcc      <  2:1804378/94‑1 (MQ=255)
                                                                                     gCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCg     >  2:2105536/1‑94 (MQ=255)
                                                                                       cAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCggg   >  1:2823991/1‑94 (MQ=255)
                                                                                       cAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCggg   >  2:4693473/1‑94 (MQ=255)
                                                                                       cAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCggg   <  2:9124253/94‑1 (MQ=255)
                                                                                        aGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGa  >  1:6299738/1‑94 (MQ=255)
                                                                                        aGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGa  >  1:6607358/1‑94 (MQ=255)
                                                                                        aGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGa  >  1:10473253/1‑94 (MQ=255)
                                                                                           |                                                                                          
GTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGA  >  NC_000913/3448600‑3448781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 320 total aligned reads displayed.
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTT  >  NC_000913/3450082‑3450268
                                                                                             |                                                                                             
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCC                                                                                               >  SRR4302545.188685/1‑94 (MQ=60)
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCC                                                                                               <  SRR4302545.8122288/94‑1 (MQ=60)
 TTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCG                                                                                              >  SRR4302545.8776666/1‑94 (MQ=60)
  TTTCTTTCCTTTGAACACTTTGCCCGGAGGCTGGTTCTTACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGG                                                                                             <  SRR4302545.3313678/94‑1 (MQ=60)
  TTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGG                                                                                             <  SRR4302545.2382566/94‑1 (MQ=60)
  TTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGG                                                                                             <  SRR4302545.5815829/94‑1 (MQ=60)
   TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGG                                                                                            >  SRR4302545.1987369/1‑94 (MQ=60)
   TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGG                                                                                            >  SRR4302545.5626143/1‑94 (MQ=60)
    TCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGT                                                                                           <  SRR4302545.1263754/94‑1 (MQ=60)
     CTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTA                                                                                          >  SRR4302545.7882484/1‑94 (MQ=60)
     CTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTA                                                                                          >  SRR4302545.8321972/1‑94 (MQ=60)
       TGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACG                                                                                        <  SRR4302545.9551393/94‑1 (MQ=60)
       TGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACG                                                                                        <  SRR4302545.5065946/94‑1 (MQ=60)
       TGCCTTTGAACACTTTGCCCGGAGTATGGTTCTGACCGATAGAACCCGGAACGCGGTGCGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACG                                                                                        <  SRR4302545.6873453/94‑1 (MQ=60)
        GCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTGGCGTCCCGGGTACGG                                                                                       <  SRR4302545.4431938/94‑1 (MQ=60)
        GCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGG                                                                                       >  SRR4302545.8369469/1‑94 (MQ=60)
        GCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGG                                                                                       >  SRR4302545.6060976/1‑94 (MQ=60)
         CCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGA                                                                                      <  SRR4302545.7424622/94‑1 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAA                                                                                     >  SRR4302545.7873204/1‑94 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAA                                                                                     <  SRR4302545.1905263/94‑1 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAA                                                                                     >  SRR4302545.4044358/1‑94 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAA                                                                                     <  SRR4302545.611138/94‑1 (MQ=60)
            TTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGT                                                                                   <  SRR4302545.3624470/94‑1 (MQ=60)
             TGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTT                                                                                  >  SRR4302545.1107751/1‑94 (MQ=60)
                ACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGTTACGGAAGTTCCA                                                                               <  SRR4302545.2243772/94‑1 (MQ=60)
                ACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCA                                                                               >  SRR4302545.467489/1‑94 (MQ=60)
                ACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCA                                                                               <  SRR4302545.3909004/94‑1 (MQ=60)
                ACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCA                                                                               <  SRR4302545.1769304/94‑1 (MQ=60)
                ACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGCTACGGAAGTTCCA                                                                               >  SRR4302545.6022941/1‑94 (MQ=60)
                 CGCTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAG                                                                              <  SRR4302545.3713290/94‑1 (MQ=60)
                 CACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAG                                                                              >  SRR4302545.124422/1‑94 (MQ=60)
                 CACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAG                                                                              >  SRR4302545.4848419/1‑94 (MQ=60)
                 CACTCTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAG                                                                              <  SRR4302545.8908752/94‑1 (MQ=60)
                  ACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGC                                                                             <  SRR4302545.2906765/94‑1 (MQ=60)
                   CTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCG                                                                            >  SRR4302545.3081324/1‑94 (MQ=60)
                   CTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCG                                                                            >  SRR4302545.6062009/1‑94 (MQ=60)
                   CTTTGCCCGGAGGCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGGAGCGTCCCGGGTACGGAAGTTCCAGCG                                                                            >  SRR4302545.3476976/1‑94 (MQ=60)
                    TTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGC                                                                           <  SRR4302545.10847979/94‑1 (MQ=60)
                     TTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCT                                                                          <  SRR4302545.8017512/94‑1 (MQ=60)
                      TGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTT                                                                         <  SRR4302545.549103/94‑1 (MQ=60)
                      TGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGCTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTT                                                                         >  SRR4302545.10399999/1‑94 (MQ=60)
                       GCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTA                                                                        >  SRR4302545.7200537/1‑94 (MQ=60)
                          CGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACG                                                                     >  SRR4302545.2184539/1‑94 (MQ=60)
                           GGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGACGTTACAGCGCTTAAAGG                                                                    >  SRR4302545.1490093/1‑94 (MQ=60)
                            GAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGT                                                                   <  SRR4302545.4070997/94‑1 (MQ=60)
                            GAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGT                                                                   >  SRR4302545.7733715/1‑94 (MQ=60)
                            GAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGT                                                                   <  SRR4302545.5279760/94‑1 (MQ=60)
                             AGTCTGGTTCTGGCCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTA                                                                  <  SRR4302545.1596844/94‑1 (MQ=60)
                             AGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTA                                                                  <  SRR4302545.10037728/94‑1 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                 >  SRR4302545.2211492/1‑94 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                 <  SRR4302545.9448634/94‑1 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                 >  SRR4302545.8620447/1‑94 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                 >  SRR4302545.1893934/1‑94 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACAGAAGTTCCAGCGCTTAACGGTAC                                                                 >  SRR4302545.7461875/1‑94 (MQ=60)
                               TCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACC                                                                <  SRR4302545.8236215/94‑1 (MQ=60)
                               TCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACA                                                                <  SRR4302545.2617214/94‑1 (MQ=60)
                                CTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCT                                                               >  SRR4302545.8824948/1‑94 (MQ=60)
                                CTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCT                                                               <  SRR4302545.4023603/94‑1 (MQ=60)
                                CTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCT                                                               <  SRR4302545.1349731/94‑1 (MQ=60)
                                 TGGTTCTGACCGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTG                                                              <  SRR4302545.2246269/94‑1 (MQ=60)
                                 TGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTG                                                              <  SRR4302545.981672/94‑1 (MQ=60)
                                 TGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTG                                                              <  SRR4302545.6595569/94‑1 (MQ=60)
                                 TGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTG                                                              <  SRR4302545.9395607/94‑1 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                             >  SRR4302545.8521629/1‑94 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                             <  SRR4302545.592508/94‑1 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                            <  SRR4302545.8461505/94‑1 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                            >  SRR4302545.10174559/1‑94 (MQ=60)
                                     TCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAA                                                          >  SRR4302545.2799146/1‑94 (MQ=60)
                                       TGACCGATAGAACCCGGAACGCGGTGAGACAAGGATTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAAC                                                        >  SRR4302545.4715315/1‑94 (MQ=60)
                                        GACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACC                                                       >  SRR4302545.4811957/1‑94 (MQ=60)
                                        GACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACC                                                       >  SRR4302545.2140363/1‑94 (MQ=60)
                                         ACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCT                                                      <  SRR4302545.1462388/94‑1 (MQ=60)
                                         ACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGGACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCT                                                      >  SRR4302545.7321246/1‑94 (MQ=60)
                                          CCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTT                                                     <  SRR4302545.430688/94‑1 (MQ=60)
                                          CCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTT                                                     <  SRR4302545.9127036/94‑1 (MQ=60)
                                           CGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    <  SRR4302545.10649198/94‑1 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    >  SRR4302545.4947633/1‑94 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    >  SRR4302545.2602457/1‑94 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    <  SRR4302545.10261243/94‑1 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    <  SRR4302545.396242/94‑1 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                    <  SRR4302545.1299525/94‑1 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTTACGGTACCTGCGAAACCTTTA                                                   <  SRR4302545.10494605/94‑1 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTA                                                   <  SRR4302545.5521278/94‑1 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTA                                                   >  SRR4302545.9976573/1‑94 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTA                                                   >  SRR4302545.2437436/1‑94 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTA                                                   >  SRR4302545.953405/1‑94 (MQ=60)
                                            GAGAGAACCCGGATCGCGCAGAGACGAGGAGTGACCATGAGTAGCGTCCCGGGTACGGAATTTCCAGCGCTTAACGGTACCAGCGAAACCTTTA                                                   <  SRR4302545.10673580/94‑1 (MQ=46)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  >  SRR4302545.4847265/1‑94 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  <  SRR4302545.2360043/94‑1 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  <  SRR4302545.10200733/94‑1 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  <  SRR4302545.10161580/94‑1 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  >  SRR4302545.1968729/1‑94 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                  >  SRR4302545.1581286/1‑94 (MQ=60)
                                              TAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACC                                                 >  SRR4302545.7818305/1‑94 (MQ=60)
                                              TAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACC                                                 >  SRR4302545.10043314/1‑94 (MQ=60)
                                              TAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACC                                                 <  SRR4302545.391863/94‑1 (MQ=60)
                                               AGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCT                                                >  SRR4302545.657845/1‑94 (MQ=60)
                                                 AACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTT                                              <  SRR4302545.2704262/94‑1 (MQ=60)
                                                 AACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTT                                              >  SRR4302545.10572594/1‑94 (MQ=60)
                                                 AACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTT                                              >  SRR4302545.4751025/1‑94 (MQ=60)
                                                  ACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTA                                             >  SRR4302545.2583096/1‑94 (MQ=60)
                                                  ACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTA                                             >  SRR4302545.7242373/1‑94 (MQ=60)
                                                  ACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTA                                             <  SRR4302545.6187434/94‑1 (MQ=60)
                                                  ACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTA                                             <  SRR4302545.9288174/94‑1 (MQ=60)
                                                   CCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAG                                            <  SRR4302545.2308310/94‑1 (MQ=60)
                                                   CCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAG                                            <  SRR4302545.453356/94‑1 (MQ=60)
                                                    CCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGA                                           <  SRR4302545.7645271/94‑1 (MQ=60)
                                                    CCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACATGCGAAACCTTTTCCTTTAGA                                           <  SRR4302545.6366043/94‑1 (MQ=60)
                                                     CGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAG                                          <  SRR4302545.2059041/94‑1 (MQ=60)
                                                     CGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAG                                          >  SRR4302545.4143236/1‑94 (MQ=60)
                                                      GGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGG                                         <  SRR4302545.3139148/94‑1 (MQ=60)
                                                      GGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGG                                         >  SRR4302545.10248282/1‑94 (MQ=60)
                                                       GAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGT                                        <  SRR4302545.6523620/94‑1 (MQ=60)
                                                       GAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGT                                        <  SRR4302545.9281020/94‑1 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                       >  SRR4302545.9169791/1‑94 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                       <  SRR4302545.8840993/94‑1 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                       <  SRR4302545.375507/94‑1 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                       <  SRR4302545.10539340/94‑1 (MQ=60)
                                                          CGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCC                                     >  SRR4302545.6950622/1‑94 (MQ=60)
                                                           GCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCA                                    <  SRR4302545.5367238/94‑1 (MQ=60)
                                                           GCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCA                                    <  SRR4302545.1455811/94‑1 (MQ=60)
                                                           GCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCA                                    >  SRR4302545.4850474/1‑94 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.4356192/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.4156258/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.2879980/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.1782624/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.1782623/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   >  SRR4302545.5282615/1‑94 (MQ=60)
                                                            CGGTGAGACAAGGAGTGACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                   <  SRR4302545.7935495/94‑1 (MQ=60)
                                                              GTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTT                                 >  SRR4302545.6079699/1‑94 (MQ=60)
                                                              GTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTT                                 <  SRR4302545.10439146/94‑1 (MQ=60)
                                                               TGAGACAGGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTA                                >  SRR4302545.7775484/1‑94 (MQ=60)
                                                               TGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTA                                >  SRR4302545.7934741/1‑94 (MQ=60)
                                                                GAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAC                               >  SRR4302545.10102341/1‑94 (MQ=60)
                                                                GAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAC                               >  SRR4302545.2054743/1‑94 (MQ=60)
                                                                GAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAC                               <  SRR4302545.1035276/94‑1 (MQ=60)
                                                                GAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAC                               <  SRR4302545.4549320/94‑1 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                              >  SRR4302545.10697148/1‑94 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                              >  SRR4302545.425225/1‑94 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                              >  SRR4302545.2537147/1‑94 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                              <  SRR4302545.2918217/94‑1 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGGTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGGGCCAGTTACG                              >  SRR4302545.7614501/1‑94 (MQ=60)
                                                                  GACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGT                             <  SRR4302545.1820507/94‑1 (MQ=60)
                                                                   ACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTATCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTC                            <  SRR4302545.1809320/94‑1 (MQ=60)
                                                                   ACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGGAACCTTTACCTTTAGAGGTGCCAGTTACGTC                            >  SRR4302545.8944362/1‑94 (MQ=60)
                                                                   ACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTA                            >  SRR4302545.10124180/1‑94 (MQ=60)
                                                                    CAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCA                           <  SRR4302545.551748/94‑1 (MQ=60)
                                                                    CAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCA                           <  SRR4302545.3383606/94‑1 (MQ=60)
                                                                    CAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCA                           <  SRR4302545.714456/94‑1 (MQ=60)
                                                                     AAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAA                          >  SRR4302545.2958153/1‑94 (MQ=60)
                                                                     AAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAA                          >  SRR4302545.1894746/1‑94 (MQ=60)
                                                                     AAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAA                          <  SRR4302545.9786086/94‑1 (MQ=60)
                                                                     AAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGGTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAA                          >  SRR4302545.4404914/1‑94 (MQ=60)
                                                                      AGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAAC                         >  SRR4302545.9788014/1‑94 (MQ=60)
                                                                      AGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAAC                         <  SRR4302545.7104130/94‑1 (MQ=60)
                                                                         AGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTT                      >  SRR4302545.1851969/1‑94 (MQ=60)
                                                                         AGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTT                      <  SRR4302545.3971430/94‑1 (MQ=60)
                                                                         AGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTT                      >  SRR4302545.9047980/1‑94 (MQ=60)
                                                                          GTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTT                     <  SRR4302545.1165594/94‑1 (MQ=60)
                                                                          GTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTT                     <  SRR4302545.6646654/94‑1 (MQ=60)
                                                                            TACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTT                   >  SRR4302545.3069965/1‑94 (MQ=60)
                                                                            TACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTT                   <  SRR4302545.7286813/94‑1 (MQ=60)
                                                                            TACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGGACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTT                   >  SRR4302545.1663763/1‑94 (MQ=60)
                                                                             ACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTA                  <  SRR4302545.8663676/94‑1 (MQ=60)
                                                                               CGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAC                <  SRR4302545.6715953/94‑1 (MQ=60)
                                                                               CGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAC                >  SRR4302545.4617822/1‑94 (MQ=60)
                                                                               CGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAC                <  SRR4302545.3443566/94‑1 (MQ=60)
                                                                               CGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAC                <  SRR4302545.3073402/94‑1 (MQ=60)
                                                                               CGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAAC                <  SRR4302545.1227425/94‑1 (MQ=60)
                                                                                GTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACG               >  SRR4302545.6163751/1‑94 (MQ=60)
                                                                                 TGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGT              <  SRR4302545.323068/94‑1 (MQ=60)
                                                                                 TGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGT              <  SRR4302545.3696359/94‑1 (MQ=60)
                                                                                  GAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTC             <  SRR4302545.6882810/94‑1 (MQ=60)
                                                                                   AGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCTACTTTTTTAACGTCA            <  SRR4302545.6344079/94‑1 (MQ=60)
                                                                                   AGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCA            <  SRR4302545.2019514/94‑1 (MQ=60)
                                                                                   AGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCA            <  SRR4302545.3019454/94‑1 (MQ=60)
                                                                                    GTAGCGTCCCGAGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTGAG           <  SRR4302545.9261627/94‑1 (MQ=60)
                                                                                     TAGCGTCCCGGGTACTGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGC          <  SRR4302545.7587497/94‑1 (MQ=60)
                                                                                      AGCGTCCCGGGTGCGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCA         <  SRR4302545.5919537/94‑1 (MQ=60)
                                                                                       GCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAA        <  SRR4302545.8209079/94‑1 (MQ=60)
                                                                                        CGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGCGCCAGTTACGTCAACTTTTTTAACGTCAGCAAA       <  SRR4302545.6698602/94‑1 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC      <  SRR4302545.9259990/94‑1 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC      >  SRR4302545.6830074/1‑94 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC      <  SRR4302545.10616976/94‑1 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTGTATTAACGTCAGCAAAC      >  SRR4302545.9649161/1‑94 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGGACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCACCTTTTTTCACGTCAGCAAAC      >  SRR4302545.2928222/1‑94 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGAGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC      >  SRR4302545.6830073/1‑94 (MQ=60)
                                                                                          TCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACA     <  SRR4302545.9351213/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG    <  SRR4302545.3269011/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG    <  SRR4302545.4318085/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG    >  SRR4302545.9903775/1‑94 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG    <  SRR4302545.6192954/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG    <  SRR4302545.8911606/94‑1 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT   <  SRR4302545.8101712/94‑1 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT   >  SRR4302545.5763431/1‑94 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT   <  SRR4302545.154922/94‑1 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT   <  SRR4302545.2444830/94‑1 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT   >  SRR4302545.8736345/1‑94 (MQ=60)
                                                                                             CGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAACCCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTT  <  SRR4302545.72183/94‑1 (MQ=60)
                                                                                             CGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTT  >  SRR4302545.5473337/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGTT  >  NC_000913/3450082‑3450268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑