Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,175 T→C Q130R (CAG→CGG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1750TC100.0% 843.7 / NA 242Q130R (CAG→CGG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/144);  total (98/144)

Only 100 of 248 total aligned reads displayed.

TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC  >  NC_000913/3450085‑3450264
                                                                                          |                                                                                         
ttCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCgg                                                                                         >  2:1726208/1‑93 (MQ=255)
ttCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCgg                                                                                         <  1:839011/93‑1 (MQ=255)
ttCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCgg                                                                                         >  1:5526249/1‑93 (MQ=255)
 tCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCggg                                                                                        >  1:1182010/1‑93 (MQ=255)
  cTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGt                                                                                       >  2:3010408/1‑93 (MQ=255)
    tGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTAc                                                                                     <  2:3725205/93‑1 (MQ=255)
    tGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTAc                                                                                     >  1:6380714/1‑93 (MQ=255)
      ccTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACgg                                                                                   >  1:4313948/1‑93 (MQ=255)
       cTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGa                                                                                  <  2:3231973/93‑1 (MQ=255)
        tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACggca                                                                                 >  1:3504976/1‑91 (MQ=255)
        tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                 <  2:612740/93‑1 (MQ=255)
        tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                 <  1:2102552/93‑1 (MQ=255)
        tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                 >  1:2211557/1‑93 (MQ=255)
        tttGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGaa                                                                                 <  1:6429071/93‑1 (MQ=255)
          tGAACTCTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGt                                                                               <  2:5989314/93‑1 (MQ=255)
           gAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGtt                                                                              <  1:3804084/93‑1 (MQ=255)
            aaCACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTc                                                                             >  1:5835858/1‑93 (MQ=255)
              cacTTTGCCCGGAGTCTGGTTCTGACCGATAGAACTCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCa                                                                           <  2:259871/93‑1 (MQ=255)
              cacTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCa                                                                           >  2:2823043/1‑93 (MQ=255)
               acTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAg                                                                          <  1:5929548/93‑1 (MQ=255)
               acTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCAAg                                                                          >  1:1709355/1‑93 (MQ=255)
                cTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAgc                                                                         >  1:4612773/1‑93 (MQ=255)
                  ttGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAgcgc                                                                       <  2:3828859/93‑1 (MQ=255)
                      ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTaa                                                                   >  2:1500312/1‑93 (MQ=255)
                      ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTaa                                                                   <  2:5118340/93‑1 (MQ=255)
                      ccGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTaa                                                                   <  1:4105766/93‑1 (MQ=255)
                       cGGAGTCTGGTTCTGACCGATAGAACCCGGCACGCGGTGAGACAAGGAGCTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                  >  1:2326286/1‑93 (MQ=255)
                       cGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                  <  1:6183820/93‑1 (MQ=255)
                       cGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAc                                                                  <  2:4481748/93‑1 (MQ=255)
                        ggAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACATGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACg                                                                 <  2:6381006/93‑1 (MQ=255)
                        ggAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACg                                                                 >  1:1675784/1‑93 (MQ=255)
                        ggAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGGTACCGTGAGGAGCGTCCCAGGTACGGAAGTTCCAGCGCTTAACg                                                                 >  2:3190068/1‑93 (MQ=255)
                         gAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACgg                                                                <  2:1902387/93‑1 (MQ=255)
                           gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTa                                                              >  1:4086814/1‑93 (MQ=255)
                           gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTa                                                              <  1:2798575/93‑1 (MQ=255)
                           gTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTa                                                              <  2:395258/93‑1 (MQ=255)
                            tCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAc                                                             >  2:5694286/1‑93 (MQ=255)
                            tCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAc                                                             >  2:5383928/1‑93 (MQ=255)
                             cTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAcc                                                            <  2:2014966/93‑1 (MQ=255)
                               ggTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGTGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTg                                                          <  1:1786890/93‑1 (MQ=255)
                               ggTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTg                                                          <  1:1311957/93‑1 (MQ=255)
                                gTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGc                                                         <  1:1007973/93‑1 (MQ=255)
                                gTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGc                                                         <  1:3735044/93‑1 (MQ=255)
                                 ttCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCg                                                        >  1:1097102/1‑93 (MQ=255)
                                    tGACCGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGaaa                                                     <  1:719586/93‑1 (MQ=255)
                                    tGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGaaa                                                     >  2:3800306/1‑93 (MQ=255)
                                     gACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAac                                                    >  2:5025586/1‑93 (MQ=255)
                                     gACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAac                                                    >  2:1018195/1‑93 (MQ=255)
                                       ccGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacct                                                  <  1:3865007/93‑1 (MQ=255)
                                         gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttt                                                >  2:1628968/1‑93 (MQ=255)
                                         gATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctgt                                                <  2:3371682/93‑3 (MQ=255)
                                          aTAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttta                                               <  1:3638804/93‑1 (MQ=255)
                                          aTAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAaccttta                                               <  1:3152710/93‑1 (MQ=255)
                                             gAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacct                                            >  2:1240156/1‑93 (MQ=255)
                                             gAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAacctttacct                                            >  2:5576542/1‑93 (MQ=255)
                                                cccGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAAcctttaccttta                                         <  2:3729280/93‑1 (MQ=255)
                                                 ccGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTag                                        <  1:3796488/93‑1 (MQ=255)
                                                   ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTagag                                      <  1:5871531/93‑1 (MQ=255)
                                                   ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTagag                                      >  2:3894144/1‑93 (MQ=255)
                                                   ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTagag                                      <  2:1250562/93‑1 (MQ=255)
                                                   ggAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGGACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTagag                                      >  1:1588387/1‑93 (MQ=255)
                                                    gAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAgg                                     >  2:3078004/1‑93 (MQ=255)
                                                     aaCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGt                                    <  2:4938869/93‑1 (MQ=255)
                                                     aaCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGt                                    <  2:6270096/93‑1 (MQ=255)
                                                     aaCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGt                                    >  2:3415287/1‑93 (MQ=255)
                                                      aCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTg                                   <  1:1533467/93‑1 (MQ=255)
                                                      aCGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTg                                   >  1:5565622/1‑93 (MQ=255)
                                                        gcgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGcc                                 <  1:1437251/93‑1 (MQ=255)
                                                         cgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCa                                <  2:5172285/93‑1 (MQ=255)
                                                         cgGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCa                                <  1:5759802/93‑1 (MQ=255)
                                                          ggTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAg                               >  2:4074232/1‑93 (MQ=255)
                                                            tGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGtt                             >  1:4904784/1‑93 (MQ=255)
                                                              agaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAc                           <  2:3869529/93‑1 (MQ=255)
                                                               gaCAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACg                          >  2:3613094/1‑93 (MQ=255)
                                                                 cAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTc                        >  2:2838783/1‑93 (MQ=255)
                                                                 cAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTc                        >  1:2076785/1‑93 (MQ=255)
                                                                  aaGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCa                       <  2:351607/93‑1 (MQ=255)
                                                                  aaGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCa                       >  1:4247852/1‑93 (MQ=255)
                                                                  aaGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCa                       <  2:2502403/93‑1 (MQ=255)
                                                                   aGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCaa                      >  2:6061026/1‑93 (MQ=255)
                                                                   aGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCaa                      >  2:837152/1‑93 (MQ=255)
                                                                   aGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCaa                      >  1:4872045/1‑93 (MQ=255)
                                                                    ggAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAAc                     <  2:5772889/93‑1 (MQ=255)
                                                                     gAGTTACCGTGAGTAGCGTCCCGGGTACGGACGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAAcc                    <  1:6467345/93‑2 (MQ=255)
                                                                     gAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACt                    <  2:1455825/93‑1 (MQ=255)
                                                                        ttACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACtttt                 <  1:3293481/93‑1 (MQ=255)
                                                                         tACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACttttt                <  2:4266413/93‑1 (MQ=255)
                                                                         tACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACttttt                <  1:5297535/93‑1 (MQ=255)
                                                                           ccGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTa              >  1:2623681/1‑93 (MQ=255)
                                                                            cGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTaa             <  2:4714042/93‑1 (MQ=255)
                                                                              tGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACg           >  1:290669/1‑93 (MQ=255)
                                                                              tGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACg           <  1:5159600/93‑1 (MQ=255)
                                                                                aGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTc         <  2:671721/93‑1 (MQ=255)
                                                                                 gTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCa        <  1:3851370/93‑1 (MQ=255)
                                                                                 gTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCa        <  2:5821055/93‑1 (MQ=255)
                                                                                  tAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAg       <  1:1863379/93‑1 (MQ=255)
                                                                                  tAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAg       <  1:880761/93‑1 (MQ=255)
                                                                                   aGCGTCCCGGGTACGGAAGTTCCAGCGCGTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGc      <  1:6214630/93‑1 (MQ=255)
                                                                                      gTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCaaa   >  1:4509032/1‑93 (MQ=255)
                                                                                      gTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCaaa   <  1:2169256/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC  >  NC_000913/3450085‑3450264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT  >  NC_000913/3450082‑3450267
                                                                                             |                                                                                            
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCC                                                                                              >  SRR4302537.10960/1‑94 (MQ=60)
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCC                                                                                              <  SRR4302537.472665/94‑1 (MQ=60)
  TTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGG                                                                                            >  SRR4302537.5563969/1‑94 (MQ=60)
   TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGG                                                                                           <  SRR4302537.3884547/94‑1 (MQ=60)
   TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGG                                                                                           >  SRR4302537.1190233/1‑94 (MQ=60)
   TTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGG                                                                                           <  SRR4302537.844758/94‑1 (MQ=60)
     CTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTA                                                                                         >  SRR4302537.2891134/1‑94 (MQ=60)
     CTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTA                                                                                         >  SRR4302537.3054891/1‑94 (MQ=60)
      TTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTAC                                                                                        >  SRR4302537.6424214/1‑94 (MQ=60)
      TTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTAC                                                                                        <  SRR4302537.5863977/94‑1 (MQ=60)
        GCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGG                                                                                      <  SRR4302537.4131725/94‑1 (MQ=60)
        GCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGG                                                                                      >  SRR4302537.4343935/1‑94 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGCA                                                                                    >  SRR4302537.3529774/1‑94 (MQ=60)
          CTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAA                                                                                    >  SRR4302537.2227150/1‑94 (MQ=60)
           TTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAG                                                                                   <  SRR4302537.4625440/94‑1 (MQ=60)
           TTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAG                                                                                   <  SRR4302537.6472752/94‑1 (MQ=60)
           TTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAG                                                                                   <  SRR4302537.2117378/94‑1 (MQ=60)
            TTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGT                                                                                  >  SRR4302537.3344391/1‑94 (MQ=60)
            TTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGT                                                                                  <  SRR4302537.727086/94‑1 (MQ=60)
             TGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTT                                                                                 <  SRR4302537.2145332/94‑1 (MQ=60)
              GAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTC                                                                                >  SRR4302537.3273850/1‑94 (MQ=60)
              GAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTC                                                                                <  SRR4302537.3808824/94‑1 (MQ=60)
              GAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTC                                                                                <  SRR4302537.3830764/94‑1 (MQ=60)
              GAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTC                                                                                >  SRR4302537.5875854/1‑94 (MQ=60)
              GAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTC                                                                                >  SRR4302537.1081406/1‑94 (MQ=60)
               AACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCC                                                                               >  SRR4302537.5498661/1‑94 (MQ=60)
               AACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCC                                                                               >  SRR4302537.1787487/1‑94 (MQ=60)
                 CACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCAAG                                                                             >  SRR4302537.1721297/1‑94 (MQ=60)
                 CACTTTGCCCGGAGTCTGGTACTGACCGATAGAACCCGGAACGCGGTGAGACAAGTAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAG                                                                             <  SRR4302537.3940880/94‑1 (MQ=60)
                  ACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGC                                                                            <  SRR4302537.1465071/94‑1 (MQ=60)
                  ACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGC                                                                            <  SRR4302537.5970237/94‑1 (MQ=60)
                  ACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGC                                                                            >  SRR4302537.4644712/1‑94 (MQ=60)
                  ACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGC                                                                            >  SRR4302537.6271174/1‑94 (MQ=60)
                   CTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGCCCCGGGTACGGAAGTTCCAGCG                                                                           >  SRR4302537.2095626/1‑94 (MQ=60)
                    TTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGC                                                                          <  SRR4302537.1448974/94‑1 (MQ=60)
                     TGGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCT                                                                         <  SRR4302537.1246660/94‑1 (MQ=60)
                      TGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACTGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTT                                                                        <  SRR4302537.609060/94‑1 (MQ=60)
                        CCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAA                                                                      <  SRR4302537.3555696/94‑1 (MQ=60)
                         CCGGAGTCTGGTTCTGACCGATAGAACCCGGCACGCGGTGAGACAAGGAGCTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAC                                                                     >  SRR4302537.2342697/1‑94 (MQ=60)
                         CCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAC                                                                     <  SRR4302537.4134444/94‑1 (MQ=60)
                         CCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAAC                                                                     >  SRR4302537.4240473/1‑94 (MQ=60)
                          CGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACG                                                                    >  SRR4302537.6446243/1‑94 (MQ=60)
                          CGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACG                                                                    >  SRR4302537.1687514/1‑94 (MQ=60)
                          CGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACG                                                                    <  SRR4302537.6226416/94‑1 (MQ=60)
                           GGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGG                                                                   >  SRR4302537.4403990/1‑94 (MQ=60)
                             AGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTA                                                                 <  SRR4302537.3756639/94‑1 (MQ=60)
                             AGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTA                                                                 >  SRR4302537.4115353/1‑94 (MQ=60)
                             AGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGCAAGTTCCAGCGgttactgttc                                                                 >  SRR4302537.647733/1‑84 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                <  SRR4302537.4187284/94‑1 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                <  SRR4302537.1966503/94‑1 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                >  SRR4302537.1708950/1‑94 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                <  SRR4302537.5531336/94‑1 (MQ=60)
                              GTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTAC                                                                <  SRR4302537.2818141/94‑1 (MQ=60)
                               TCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACC                                                               <  SRR4302537.3692241/94‑1 (MQ=60)
                                CTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCT                                                              <  SRR4302537.5907842/94‑1 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGTGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                            <  SRR4302537.1799375/94‑1 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                            <  SRR4302537.1321086/94‑1 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                            <  SRR4302537.5929213/94‑1 (MQ=60)
                                  GGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGC                                                            >  SRR4302537.6499750/1‑94 (MQ=60)
                                   GTTCTGACCGATAGAGCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                           <  SRR4302537.1380813/94‑1 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                           <  SRR4302537.3761279/94‑1 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                           <  SRR4302537.845688/94‑1 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                           >  SRR4302537.1104683/1‑94 (MQ=60)
                                   GTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCG                                                           <  SRR4302537.1014928/94‑1 (MQ=60)
                                    TTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGA                                                          >  SRR4302537.435469/1‑94 (MQ=60)
                                    TTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCCGGGACGGAAGTTCCAGCGCTTAACGGCACCTGCGA                                                          >  SRR4302537.4305912/1‑94 (MQ=60)
                                    GTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGA                                                          <  SRR4302537.401942/94‑1 (MQ=60)
                                      CTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAA                                                        >  SRR4302537.721484/1‑94 (MQ=60)
                                       TGACCGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAAC                                                       <  SRR4302537.724501/94‑1 (MQ=60)
                                       TGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCTGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAAC                                                       >  SRR4302537.1658059/1‑94 (MQ=60)
                                         ACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCT                                                     <  SRR4302537.4905894/94‑1 (MQ=60)
                                          CCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTT                                                    <  SRR4302537.3892060/94‑1 (MQ=60)
                                          CCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTT                                                    <  SRR4302537.3026912/94‑1 (MQ=60)
                                           CGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTT                                                   >  SRR4302537.5128259/1‑94 (MQ=60)
                                            GATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTA                                                  >  SRR4302537.5690311/1‑94 (MQ=60)
                                            GAGAGAACCCGAAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTAAACGGTACCTGCGAAACCTTTA                                                  <  SRR4302537.485417/94‑1 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                 <  SRR4302537.3664425/94‑1 (MQ=60)
                                             ATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTAC                                                 <  SRR4302537.3174846/94‑1 (MQ=60)
                                               AGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCT                                               <  SRR4302537.1600624/94‑1 (MQ=60)
                                               AGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCT                                               >  SRR4302537.2807413/1‑94 (MQ=60)
                                                 AACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTTCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTT                                             <  SRR4302537.3436172/94‑1 (MQ=60)
                                                 AACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTT                                             <  SRR4302537.4733001/94‑1 (MQ=60)
                                                    CCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGA                                          <  SRR4302537.3823125/94‑1 (MQ=60)
                                                    CCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGA                                          <  SRR4302537.5082588/94‑1 (MQ=60)
                                                     CGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGGACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAG                                         >  SRR4302537.1599490/1‑94 (MQ=60)
                                                      GGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGG                                        <  SRR4302537.5989317/94‑1 (MQ=60)
                                                      GGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGG                                        <  SRR4302537.5911806/94‑1 (MQ=60)
                                                       GAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGT                                       <  SRR4302537.5667776/94‑1 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                      >  SRR4302537.5603661/1‑94 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                      >  SRR4302537.5308114/1‑94 (MQ=60)
                                                        AACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTG                                      <  SRR4302537.1861031/94‑1 (MQ=60)
                                                         ACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGC                                     <  SRR4302537.1544181/94‑1 (MQ=60)
                                                           GCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCA                                   <  SRR4302537.2209777/94‑1 (MQ=60)
                                                           GCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCA                                   <  SRR4302537.1447245/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                  <  SRR4302537.5799253/94‑1 (MQ=60)
                                                            CGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAG                                  >  SRR4302537.1745030/1‑94 (MQ=60)
                                                              GTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTT                                <  SRR4302537.5596341/94‑1 (MQ=60)
                                                              GTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTT                                >  SRR4302537.4938639/1‑94 (MQ=60)
                                                              GTGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTT                                <  SRR4302537.6497459/94‑1 (MQ=60)
                                                               TGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAGCCTTTACCTTTAGAGGTGCCAGTTA                               >  SRR4302537.207882/1‑94 (MQ=60)
                                                               TGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTA                               >  SRR4302537.1187174/1‑94 (MQ=60)
                                                               TGAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTA                               <  SRR4302537.2335668/94‑1 (MQ=60)
                                                                GAGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTAC                              <  SRR4302537.3824777/94‑1 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                             >  SRR4302537.1295860/1‑94 (MQ=60)
                                                                 AGACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACG                             <  SRR4302537.590230/94‑1 (MQ=60)
                                                                   ACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTC                           >  SRR4302537.2091411/1‑94 (MQ=60)
                                                                   ACAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTC                           >  SRR4302537.6405855/1‑94 (MQ=60)
                                                                    CAAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCA                          >  SRR4302537.4277372/1‑94 (MQ=60)
                                                                     AAGGAGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAA                         >  SRR4302537.4905672/1‑94 (MQ=60)
                                                                        GAGTTACCGTGAGTAGCGTCCCGGGTACGGACGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACCT                      <  SRR4302537.6511164/94‑1 (MQ=60)
                                                                         AGTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTT                     <  SRR4302537.3845684/94‑1 (MQ=60)
                                                                          GTTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTT                    <  SRR4302537.993221/94‑1 (MQ=60)
                                                                           TTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTT                   >  SRR4302537.3825461/1‑94 (MQ=60)
                                                                           TTACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTT                   <  SRR4302537.3316760/94‑1 (MQ=60)
                                                                            TACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTT                  <  SRR4302537.5333925/94‑1 (MQ=60)
                                                                             ACCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTA                 >  SRR4302537.2642069/1‑94 (MQ=60)
                                                                              CCGTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAA                <  SRR4302537.6243771/94‑1 (MQ=60)
                                                                                GTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACG              >  SRR4302537.1587903/1‑94 (MQ=60)
                                                                                GTGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACG              >  SRR4302537.292694/1‑94 (MQ=60)
                                                                                 TGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGT             <  SRR4302537.5195159/94‑1 (MQ=60)
                                                                                 TGAGTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGT             <  SRR4302537.2662709/94‑1 (MQ=60)
                                                                                    GTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAG          <  SRR4302537.3878337/94‑1 (MQ=60)
                                                                                    GTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAG          <  SRR4302537.828312/94‑1 (MQ=60)
                                                                                    GTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAG          <  SRR4302537.2365773/94‑1 (MQ=60)
                                                                                    GTAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAG          <  SRR4302537.2319302/94‑1 (MQ=60)
                                                                                     TAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGC         <  SRR4302537.1876355/94‑1 (MQ=60)
                                                                                     TAGCGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGC         <  SRR4302537.886821/94‑1 (MQ=60)
                                                                                      AGCGTCCCGGGTACGGAAGTTCCAGCGCGTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCA        <  SRR4302537.6257426/94‑1 (MQ=60)
                                                                                        CGTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAA      >  SRR4302537.4540210/1‑94 (MQ=60)
                                                                                         GTCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAAC     <  SRR4302537.2184538/94‑1 (MQ=60)
                                                                                          TCCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACA    <  SRR4302537.2118894/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG   <  SRR4302537.6294356/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG   <  SRR4302537.4207912/94‑1 (MQ=60)
                                                                                           CCCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAG   <  SRR4302537.869962/94‑1 (MQ=60)
                                                                                            CCGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT  <  SRR4302537.6164513/94‑1 (MQ=60)
                                                                                             |                                                                                            
ATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGGTACCTGCGAAACCTTTACCTTTAGAGGTGCCAGTTACGTCAACTTTTTTAACGTCAGCAAACAGT  >  NC_000913/3450082‑3450267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑