Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,376,055 T→C T78A (ACC→GCC)  rplM ← 50S ribosomal subunit protein L13

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,0550TC100.0% 825.4 / NA 236T78A (ACC→GCC) rplM50S ribosomal subunit protein L13
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (131/105);  total (131/105)

Only 100 of 248 total aligned reads displayed.

TGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTA  >  NC_000913/3375965‑3376144
                                                                                          |                                                                                         
  ccTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCgtgg                                                                                       <  1:4670558/93‑1 (MQ=255)
   cTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCgtggg                                                                                      >  2:4687425/1‑92 (MQ=255)
     ttAGCCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGa                                                                                    <  1:5608112/93‑1 (MQ=255)
     ttAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGa                                                                                    <  2:5061703/93‑1 (MQ=255)
       aaCCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATa                                                                                  >  1:122962/1‑93 (MQ=255)
       aaCCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATa                                                                                  >  2:3283807/1‑93 (MQ=255)
        aCCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAg                                                                                 >  2:4713568/1‑93 (MQ=255)
           gcgATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTac                                                                              >  1:6322400/1‑93 (MQ=255)
             gATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTacat                                                                            >  1:1039299/1‑92 (MQ=255)
              aTTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACt                                                                           >  2:2165887/1‑93 (MQ=255)
               tttCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                          <  2:423004/93‑1 (MQ=255)
               tttCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACtt                                                                          >  1:898549/1‑93 (MQ=255)
                ttCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACttt                                                                         <  2:5134598/93‑1 (MQ=255)
                 tCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTg                                                                        <  2:6214899/93‑1 (MQ=255)
                  cAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGt                                                                       <  1:2767356/93‑1 (MQ=255)
                  cAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGt                                                                       >  2:3185203/1‑93 (MQ=255)
                   aaTCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTc                                                                      <  1:278034/93‑1 (MQ=255)
                    aTCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCa                                                                     >  1:1735680/1‑93 (MQ=255)
                     tCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAg                                                                    <  1:2428483/93‑1 (MQ=255)
                     tCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAg                                                                    >  2:1487996/1‑93 (MQ=255)
                     tCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAg                                                                    <  2:2385019/93‑1 (MQ=255)
                       acacGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTa                                                                  <  2:3084251/93‑1 (MQ=255)
                          cGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGc                                                               <  2:1447229/93‑1 (MQ=255)
                           gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCt                                                              >  1:3154128/1‑93 (MQ=255)
                           gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCt                                                              >  2:1307173/1‑93 (MQ=255)
                           gCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGCACGCt                                                              >  1:434525/1‑93 (MQ=255)
                             tCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttg                                                            <  1:5690335/93‑1 (MQ=255)
                              cAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttgt                                                           <  2:4808403/93‑1 (MQ=255)
                               aGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttgtt                                                          <  1:1903761/93‑1 (MQ=255)
                                ggACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCttgttg                                                         >  1:3539233/1‑93 (MQ=255)
                                 gtcGGCGAGCAATCATCTCTTCAAAGGTCGCGTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGc                                                        <  2:940681/91‑1 (MQ=255)
                                 gACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGc                                                        >  1:6088507/1‑93 (MQ=255)
                                 gACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGc                                                        >  1:2891738/1‑93 (MQ=255)
                                  aCGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATGCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGcc                                                       <  1:5787071/93‑1 (MQ=255)
                                   cGGCGAGCCATCATCTCTTTAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCg                                                      >  2:6328215/1‑93 (MQ=255)
                                   cGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCg                                                      <  1:710552/93‑1 (MQ=255)
                                     gCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGt                                                    <  2:6020317/93‑1 (MQ=255)
                                      ggAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGtt                                                   <  1:4842437/92‑1 (MQ=255)
                                      cGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGtt                                                   >  2:87923/1‑93 (MQ=255)
                                        aGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTAc                                                 <  2:1866961/93‑1 (MQ=255)
                                        aGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTAc                                                 >  1:4615161/1‑93 (MQ=255)
                                         gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACa                                                >  1:2856328/1‑93 (MQ=255)
                                         gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACa                                                >  1:682206/1‑93 (MQ=255)
                                         gCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACa                                                >  2:706820/1‑93 (MQ=255)
                                           aaTCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGc                                              >  2:3426988/1‑93 (MQ=255)
                                           aaTCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGc                                              <  2:1153173/93‑1 (MQ=255)
                                            atcatcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCa                                             >  2:5005267/1‑93 (MQ=255)
                                               atcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACt                                          >  1:350626/1‑93 (MQ=255)
                                               atcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACt                                          <  1:4481601/93‑1 (MQ=255)
                                               atcTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACt                                          >  1:1386288/1‑93 (MQ=255)
                                                tctcTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACtt                                         <  1:3079791/93‑1 (MQ=255)
                                                tctcTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACtt                                         >  2:5691360/1‑93 (MQ=255)
                                                tctcTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACtt                                         <  2:4970361/93‑1 (MQ=255)
                                                 ctctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACttt                                        <  1:2626593/93‑1 (MQ=255)
                                                  tctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTg                                       >  2:3110636/1‑93 (MQ=255)
                                                   ctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGt                                      >  1:2020464/1‑93 (MQ=255)
                                                   ctTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGt                                      <  1:6153468/93‑1 (MQ=255)
                                                     tCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                    <  2:5769853/93‑1 (MQ=255)
                                                     tCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                    <  2:2943133/93‑1 (MQ=255)
                                                     tCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                    >  2:1069887/1‑93 (MQ=255)
                                                     tCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                    >  1:4935606/1‑93 (MQ=255)
                                                     tCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCa                                    >  1:4370842/1‑93 (MQ=255)
                                                      cAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAg                                   <  2:4349700/93‑1 (MQ=255)
                                                      cAAAGGTCGCTTGTTTGATACCACCGATGTAGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAAATTTGTCAg                                   >  2:1776459/1‑93 (MQ=255)
                                                       aaaGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGc                                  >  2:2466606/1‑93 (MQ=255)
                                                        aaGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCg                                 >  1:2194274/1‑93 (MQ=255)
                                                           gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTc                              >  2:5940268/1‑93 (MQ=255)
                                                           gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTc                              >  1:4024451/1‑93 (MQ=255)
                                                           gTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTc                              >  2:4274658/1‑93 (MQ=255)
                                                            tCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCa                             >  2:6464274/1‑93 (MQ=255)
                                                            tCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCa                             <  1:5167255/93‑1 (MQ=255)
                                                             cGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAg                            <  1:2029080/93‑1 (MQ=255)
                                                              ggttGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGATACAGCAACTTTGTCAGCGTTCAGa                           >  1:6249489/3‑93 (MQ=255)
                                                              gCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGa                           <  2:4535719/93‑1 (MQ=255)
                                                               cTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGaa                          >  2:5310909/1‑93 (MQ=255)
                                                               cTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGaa                          <  2:2759625/93‑1 (MQ=255)
                                                                ttGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAAc                         <  1:4282090/93‑1 (MQ=255)
                                                                ttGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAAc                         >  2:2615052/1‑93 (MQ=255)
                                                                  gTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACga                       <  1:502780/93‑1 (MQ=255)
                                                                   tttGATGCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgat                      <  1:2137968/93‑1 (MQ=255)
                                                                   tttGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgat                      >  1:920771/1‑93 (MQ=255)
                                                                    ttGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatg                     <  1:5848353/93‑1 (MQ=255)
                                                                     tGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatgt                    >  2:3881451/1‑92 (MQ=255)
                                                                     tGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACgatga                    <  2:3717702/93‑1 (MQ=255)
                                                                          ccaccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTaa               >  1:4658468/1‑93 (MQ=255)
                                                                           caccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAAt              <  2:3493617/93‑1 (MQ=255)
                                                                           caccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAAt              >  2:5249962/1‑93 (MQ=255)
                                                                            cccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATc             <  1:3980946/92‑1 (MQ=255)
                                                                            accGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATc             >  2:3252645/1‑93 (MQ=255)
                                                                             ccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCa            >  1:1741882/1‑93 (MQ=255)
                                                                             ccGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCa            <  1:2386403/93‑1 (MQ=255)
                                                                               gATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAcc          >  2:5465003/1‑93 (MQ=255)
                                                                               gATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAcc          >  1:128929/1‑93 (MQ=255)
                                                                                 tgtgGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCgg        >  2:4012287/1‑93 (MQ=255)
                                                                                  gtgGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGt       <  2:3324645/93‑1 (MQ=255)
                                                                                   tgGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTa      <  1:5927736/93‑1 (MQ=255)
                                                                                   tgGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTa      <  2:2142329/93‑1 (MQ=255)
                                                                                     gCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATc    <  1:1199643/93‑1 (MQ=255)
                                                                                     gCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATc    <  1:5457335/93‑1 (MQ=255)
                                                                                     gCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATc    <  2:4569100/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTA  >  NC_000913/3375965‑3376144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTG  >  NC_000913/3375962‑3376148
                                                                                             |                                                                                             
ACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGC                                                                                               >  SRR4302537.5778578/1‑94 (MQ=60)
  ATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGT                                                                                             >  SRR4302537.2793034/1‑94 (MQ=60)
   TGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTG                                                                                            >  SRR4302537.176914/1‑94 (MQ=60)
   TGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTG                                                                                            <  SRR4302537.413133/94‑1 (MQ=60)
    GCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGG                                                                                           >  SRR4302537.4022171/1‑94 (MQ=60)
     CCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGT                                                                                          <  SRR4302537.4702872/94‑1 (MQ=60)
     CCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGG                                                                                          >  SRR4302537.1623068/1‑94 (MQ=60)
        TTAGCCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGAT                                                                                       <  SRR4302537.5646452/94‑1 (MQ=60)
        TTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGAT                                                                                       >  SRR4302537.1801030/1‑94 (MQ=60)
         TAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATA                                                                                      >  SRR4302537.509324/1‑94 (MQ=60)
         TAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATA                                                                                      >  SRR4302537.123849/1‑94 (MQ=60)
         TAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGGTTGATACCACCGATGTGGCCGGCGTGGTAATA                                                                                      >  SRR4302537.3813578/1‑94 (MQ=60)
          AACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAG                                                                                     >  SRR4302537.4958835/1‑94 (MQ=60)
            CCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTA                                                                                   >  SRR4302537.4378678/1‑94 (MQ=60)
            CCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTA                                                                                   >  SRR4302537.1932213/1‑94 (MQ=60)
             CGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTAC                                                                                  >  SRR4302537.6365657/1‑94 (MQ=60)
             CGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTAC                                                                                  >  SRR4302537.203869/1‑94 (MQ=60)
              GCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACA                                                                                 >  SRR4302537.353099/1‑94 (MQ=60)
               CGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACAT                                                                                >  SRR4302537.1046452/1‑94 (MQ=60)
               CGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACAC                                                                                >  SRR4302537.1240181/1‑94 (MQ=60)
               CGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACAC                                                                                >  SRR4302537.4811338/1‑94 (MQ=60)
                GATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACT                                                                               >  SRR4302537.278136/1‑94 (MQ=60)
                 ATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTT                                                                              >  SRR4302537.904744/1‑94 (MQ=60)
                  TTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTT                                                                             <  SRR4302537.4170161/94‑1 (MQ=60)
                     CAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTC                                                                          <  SRR4302537.2786699/94‑1 (MQ=60)
                      AATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCA                                                                         >  SRR4302537.1578427/1‑94 (MQ=60)
                      AATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCA                                                                         <  SRR4302537.279976/94‑1 (MQ=60)
                      AATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCA                                                                         >  SRR4302537.1747787/1‑94 (MQ=60)
                       ATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAG                                                                        >  SRR4302537.82280/1‑94 (MQ=60)
                       ATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAG                                                                        <  SRR4302537.2034933/94‑1 (MQ=60)
                        TCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATCCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGT                                                                       <  SRR4302537.5659579/94‑1 (MQ=60)
                        TCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGT                                                                       <  SRR4302537.2445592/94‑1 (MQ=60)
                          ACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTAC                                                                     <  SRR4302537.2872628/94‑1 (MQ=60)
                             CGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCT                                                                  >  SRR4302537.3176275/1‑94 (MQ=60)
                             CGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGCACGCT                                                                  >  SRR4302537.437555/1‑94 (MQ=60)
                              GCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTT                                                                 >  SRR4302537.4663202/1‑94 (MQ=60)
                                TCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGT                                                               <  SRR4302537.5729256/94‑1 (MQ=60)
                                  AGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTG                                                             <  SRR4302537.2762357/94‑1 (MQ=60)
                                  AGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTG                                                             <  SRR4302537.1916981/94‑1 (MQ=60)
                                  AGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTG                                                             >  SRR4302537.3564229/1‑94 (MQ=60)
                                  AGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTG                                                             <  SRR4302537.2952658/94‑1 (MQ=60)
                                   GGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGC                                                            >  SRR4302537.6130334/1‑94 (MQ=60)
                                   GGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGC                                                            >  SRR4302537.2912040/1‑94 (MQ=60)
                                     ACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATGCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCG                                                          <  SRR4302537.5826753/94‑1 (MQ=60)
                                      CGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGG                                                         <  SRR4302537.715416/94‑1 (MQ=60)
                                        GCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTT                                                       <  SRR4302537.1395016/94‑1 (MQ=60)
                                         GGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTA                                                      <  SRR4302537.4875861/94‑1 (MQ=60)
                                         CGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTA                                                      >  SRR4302537.6501560/1‑94 (MQ=60)
                                          GAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTAC                                                     >  SRR4302537.4647123/1‑94 (MQ=60)
                                           AGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACA                                                    >  SRR4302537.2876356/1‑94 (MQ=60)
                                           AGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACA                                                    <  SRR4302537.5732975/94‑1 (MQ=60)
                                           AGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACA                                                    >  SRR4302537.686883/1‑94 (MQ=60)
                                            ACNANCNTNTCTTCAAAGGTCGNTTGTTTGATACCACCGNTGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAG                                                   <  SRR4302537.1556307/94‑1 (MQ=60)
                                             CAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGC                                                  >  SRR4302537.2974454/1‑94 (MQ=60)
                                             CAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGC                                                  >  SRR4302537.6215500/1‑94 (MQ=60)
                                              AATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCA                                                 >  SRR4302537.6181241/1‑94 (MQ=60)
                                              AATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCA                                                 >  SRR4302537.4686124/1‑94 (MQ=60)
                                               TTCATCTCTTCAAAGGTCTTTTTTTTGCTCCCACGGATGTGGCGGGGGTGGTGATAGTACTCTTTGTCAGTACGTTTGTTGCCGGTTACAGCAA                                                <  SRR4302537.2884674/94‑1 (MQ=40)
                                                 CATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACT                                              >  SRR4302537.353061/1‑94 (MQ=60)
                                                 CATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACT                                              >  SRR4302537.1395956/1‑94 (MQ=60)
                                                  ATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTT                                             >  SRR4302537.625030/1‑94 (MQ=60)
                                                  ATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTT                                             >  SRR4302537.1239366/1‑94 (MQ=60)
                                                  ATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTT                                             <  SRR4302537.4512601/94‑1 (MQ=60)
                                                  ATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTT                                             <  SRR4302537.4343351/94‑1 (MQ=60)
                                                  ATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTT                                             >  SRR4302537.953599/1‑94 (MQ=60)
                                                   TCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTT                                            <  SRR4302537.3101240/94‑1 (MQ=60)
                                                   TCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTT                                            <  SRR4302537.5609374/94‑1 (MQ=60)
                                                    CTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTG                                           <  SRR4302537.2644994/94‑1 (MQ=60)
                                                    CTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTG                                           >  SRR4302537.707416/1‑94 (MQ=60)
                                                     TCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGT                                          >  SRR4302537.2034558/1‑94 (MQ=60)
                                                      CTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTC                                         <  SRR4302537.6195801/94‑1 (MQ=60)
                                                       TTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCA                                        >  SRR4302537.4401044/1‑94 (MQ=60)
                                                       TTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCA                                        >  SRR4302537.4969645/1‑94 (MQ=60)
                                                        TCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAG                                       >  SRR4302537.242929/1‑94 (MQ=60)
                                                          AAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCG                                     >  SRR4302537.5930748/1‑94 (MQ=60)
                                                          AAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCG                                     <  SRR4302537.2294767/94‑1 (MQ=60)
                                                          AAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCG                                     >  SRR4302537.2209734/1‑94 (MQ=60)
                                                           AAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGT                                    >  SRR4302537.4452073/1‑94 (MQ=60)
                                                           AAGGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGT                                    <  SRR4302537.29012/94‑1 (MQ=60)
                                                             GGTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTC                                  >  SRR4302537.4052626/1‑94 (MQ=60)
                                                              GTCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCA                                 >  SRR4302537.643296/1‑94 (MQ=60)
                                                               TCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAG                                <  SRR4302537.5202852/94‑1 (MQ=60)
                                                               TCGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAG                                >  SRR4302537.1753320/1‑94 (MQ=60)
                                                                CGGTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGATACAGCAACTTTGTCAGCGTTCAGA                               >  SRR4302537.6292422/1‑94 (MQ=60)
                                                                CGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGA                               >  SRR4302537.2442250/1‑94 (MQ=60)
                                                                CGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGA                               <  SRR4302537.2043226/94‑1 (MQ=60)
                                                                CGCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGA                               >  SRR4302537.3131662/1‑94 (MQ=60)
                                                                 GCTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAA                              >  SRR4302537.2101641/1‑94 (MQ=60)
                                                                  CTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAAC                             >  SRR4302537.6006449/1‑94 (MQ=60)
                                                                  CTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAAC                             >  SRR4302537.1136853/1‑94 (MQ=60)
                                                                  CTTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAAC                             >  SRR4302537.4532814/1‑94 (MQ=60)
                                                                   TTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACG                            >  SRR4302537.220316/1‑94 (MQ=60)
                                                                   TTGTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACG                            <  SRR4302537.4311857/94‑1 (MQ=60)
                                                                     GTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGAT                          <  SRR4302537.506236/94‑1 (MQ=60)
                                                                     GTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGAT                          >  SRR4302537.927124/1‑94 (MQ=60)
                                                                     GTTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGAT                          >  SRR4302537.5661912/1‑94 (MQ=60)
                                                                      TTTGATGCCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATG                         <  SRR4302537.2153017/94‑1 (MQ=60)
                                                                      TTTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATG                         <  SRR4302537.6040694/94‑1 (MQ=60)
                                                                       TTGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGA                        <  SRR4302537.5888457/94‑1 (MQ=60)
                                                                        TGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGAT                       <  SRR4302537.6309027/94‑1 (MQ=60)
                                                                        TGATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGAT                       >  SRR4302537.4448325/1‑94 (MQ=60)
                                                                         GATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATG                      >  SRR4302537.5016805/1‑94 (MQ=60)
                                                                          ATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGT                     <  SRR4302537.1230547/94‑1 (MQ=60)
                                                                          ATACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGT                     <  SRR4302537.325307/94‑1 (MQ=60)
                                                                            ACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAA                   >  SRR4302537.4690675/1‑94 (MQ=60)
                                                                            ACCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAA                   <  SRR4302537.4990246/94‑1 (MQ=60)
                                                                             CCACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAAT                  >  SRR4302537.914240/1‑94 (MQ=60)
                                                                              CACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATC                 >  SRR4302537.4777962/1‑94 (MQ=60)
                                                                              CACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATC                 <  SRR4302537.83695/94‑1 (MQ=60)
                                                                               CCCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCA                <  SRR4302537.4008814/94‑1 (MQ=60)
                                                                               ACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCA                >  SRR4302537.5266383/1‑94 (MQ=60)
                                                                               ACCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCA                >  SRR4302537.1754026/1‑94 (MQ=60)
                                                                                CCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAC               >  SRR4302537.3801114/1‑94 (MQ=60)
                                                                                CCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAC               <  SRR4302537.5422554/94‑1 (MQ=60)
                                                                                CCGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCAC               <  SRR4302537.2403210/94‑1 (MQ=60)
                                                                                 CGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACC              >  SRR4302537.3461672/1‑94 (MQ=60)
                                                                                 CGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACC              >  SRR4302537.129845/1‑94 (MQ=60)
                                                                                 CGATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACC              <  SRR4302537.2858469/94‑1 (MQ=60)
                                                                                  GATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCG             >  SRR4302537.2582109/1‑94 (MQ=60)
                                                                                  GATGTGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCG             >  SRR4302537.5830324/1‑94 (MQ=60)
                                                                                      TGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCCCCGGTAT         <  SRR4302537.584437/94‑1 (MQ=60)
                                                                                      TGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTAT         <  SRR4302537.5968415/94‑1 (MQ=60)
                                                                                      TGGCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTAT         <  SRR4302537.2180525/94‑1 (MQ=60)
                                                                                        GCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCT       <  SRR4302537.1207980/94‑1 (MQ=60)
                                                                                        GCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCT       <  SRR4302537.5494558/94‑1 (MQ=60)
                                                                                        GCCGGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCT       <  SRR4302537.4691511/94‑1 (MQ=60)
                                                                                           GGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACG    >  SRR4302537.5416096/1‑94 (MQ=60)
                                                                                           GGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACG    >  SRR4302537.4705060/1‑94 (MQ=60)
                                                                                           GGCGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACG    >  SRR4302537.5869995/1‑94 (MQ=60)
                                                                                             CGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTG  >  SRR4302537.3306129/1‑94 (MQ=60)
                                                                                             CGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTG  <  SRR4302537.6197185/94‑1 (MQ=60)
                                                                                             CGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTG  >  SRR4302537.4325033/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACATGCCTTTAACCGCGATTTCAATCACACGCTCAGGACGGCGAGCAATCATCTCTTCAAAGGTCGCTTGTTTGATACCACCGATGTGGCCGGTGTGGTGATAGTACACTTTGTCAGTACGCTTGTTGCCGGTTACAGCAACTTTGTCAGCGTTCAGAACGATGATGTAATCACCGGTATCTACGTG  >  NC_000913/3375962‑3376148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑