Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,588,160 A→G F128S (TTC→TCC)  ugpA ← UgpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,1600AG99.6% 843.0 / ‑5.1 240F128S (TTC→TCC) ugpAUgpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (115/124);  minor base T (1/0);  total (116/124)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 255 total aligned reads displayed.

ATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTA  >  NC_000913/3588070‑3588248
                                                                                          |                                                                                        
 ttGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGgaga                                                                                       <  1:982110/93‑1 (MQ=255)
 ttGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGgaga                                                                                       <  2:2651025/93‑1 (MQ=255)
 ttGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGgaga                                                                                       <  2:3468571/93‑1 (MQ=255)
  tGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGAt                                                                                      <  2:5151641/93‑1 (MQ=255)
    ccTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATcc                                                                                    >  2:4088826/1‑93 (MQ=255)
    ccTGACAGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATcc                                                                                    <  1:1258888/93‑1 (MQ=255)
        cccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACaa                                                                                <  2:4702244/92‑1 (MQ=255)
        aCCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACaa                                                                                <  1:6240107/93‑1 (MQ=255)
         ccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAAt                                                                               >  2:5074837/1‑93 (MQ=255)
          cGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                              >  1:561965/1‑93 (MQ=255)
          cGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                              >  1:198141/1‑93 (MQ=255)
          cGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                              >  2:3879089/1‑93 (MQ=255)
          cGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                              >  2:2423031/1‑93 (MQ=255)
          cGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGGTAAACAGGGAGATCCACAATa                                                                              >  1:3996269/1‑93 (MQ=255)
           gCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAc                                                                             >  2:4670107/1‑93 (MQ=255)
           gCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAc                                                                             <  1:3842781/93‑1 (MQ=255)
            cTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcg                                                                            <  1:2251271/93‑1 (MQ=255)
              gTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcggc                                                                          <  2:1810929/93‑1 (MQ=255)
              gTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcggc                                                                          >  1:4842488/1‑93 (MQ=255)
                 ttGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACggcggc                                                                       >  1:323275/1‑93 (MQ=255)
                      cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCaagcg                                                                  >  1:5485658/1‑90 (MQ=255)
                      cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcgg                                                                  >  2:3086799/1‑93 (MQ=255)
                      cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcgg                                                                  <  1:1035326/93‑1 (MQ=255)
                      cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcgg                                                                  <  2:5912571/93‑1 (MQ=255)
                      cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcgg                                                                  <  2:5267558/93‑1 (MQ=255)
                        tGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggcg                                                                >  2:2285063/1‑93 (MQ=255)
                         ggTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggcgg                                                               <  1:2052140/93‑1 (MQ=255)
                          gTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCggg                                                              <  1:31721/93‑1 (MQ=255)
                          gTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCggg                                                              >  1:2122113/1‑93 (MQ=255)
                           ttCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGa                                                             <  2:2981581/93‑1 (MQ=255)
                           ttCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGa                                                             >  1:2472266/1‑93 (MQ=255)
                            tCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGTAGATCCACAATACGGCGGCAACGGCGGGAg                                                            >  2:5768682/1‑93 (MQ=255)
                            tCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAg                                                            >  2:3069553/1‑93 (MQ=255)
                             ccAGTTGTAGGCTATCTCCGCGAGAAAATGGGTGATCAGTCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGc                                                           <  2:1550482/93‑1 (MQ=255)
                             ccAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGc                                                           <  2:4007869/93‑1 (MQ=255)
                             ccAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGAAACGGCgggggg                                                           >  1:5605445/1‑90 (MQ=255)
                              cAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGcc                                                          <  1:1712851/93‑1 (MQ=255)
                              cAGTCGTAGCCGAACTCCGCGAGAAAATGGGGGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGcc                                                          >  2:1870641/1‑93 (MQ=255)
                                gTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCAc                                                        <  1:5991351/93‑1 (MQ=255)
                                gTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCAc                                                        <  2:2040817/93‑1 (MQ=255)
                                  cGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACgg                                                      <  1:5155539/93‑1 (MQ=255)
                                       ccGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTaa                                                 <  2:5413725/93‑1 (MQ=255)
                                        cGAACTCCGCGATAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAg                                                <  1:694319/93‑1 (MQ=255)
                                        cGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCGGCgcgag                                                >  1:253063/1‑89 (MQ=255)
                                        cGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAg                                                <  1:3428599/93‑1 (MQ=255)
                                        cGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGTAAg                                                >  1:2856965/1‑93 (MQ=255)
                                           aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAggga                                             >  2:5562757/1‑91 (MQ=255)
                                           aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgca                                             <  1:5861445/93‑1 (MQ=255)
                                           aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgca                                             <  1:2894245/93‑1 (MQ=255)
                                           aCTCCGCGAGAAAAGGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgca                                             <  1:1298140/93‑1 (MQ=255)
                                            cTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGGCCCGGCGGAAAgcag                                            >  2:3508206/1‑93 (MQ=255)
                                             tCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCGGCGGAAGgcagc                                           >  1:3815463/1‑93 (MQ=255)
                                             tCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgcagc                                           <  2:3267663/93‑1 (MQ=255)
                                                 cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACa                                       <  2:1657735/93‑1 (MQ=255)
                                                 cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACa                                       <  2:6124829/93‑1 (MQ=255)
                                                 cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACa                                       <  2:2124384/93‑1 (MQ=255)
                                                  gagaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAt                                      <  1:7724/93‑1 (MQ=255)
                                                  gagaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAt                                      <  1:4181636/93‑1 (MQ=255)
                                                   agaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAtt                                     >  1:1630335/1‑93 (MQ=255)
                                                   agaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAtt                                     <  1:3107327/93‑1 (MQ=255)
                                                    gaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTa                                    <  1:1359426/93‑1 (MQ=255)
                                                    gaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGTGAGGCAGCAACAATa                                    >  1:105524/1‑93 (MQ=255)
                                                     aaaaTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCTACGGCGTAAGGCAGCAACATTaa                                   <  1:87334/93‑1 (MQ=255)
                                                     aaaaTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTaa                                   <  1:4935726/93‑1 (MQ=255)
                                                      aaaTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAg                                  <  2:2780999/93‑1 (MQ=255)
                                                        aTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGTAAGGCAGCAACAATAAGGt                                >  2:4234453/1‑93 (MQ=255)
                                                        aTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGt                                <  2:1243292/93‑1 (MQ=255)
                                                        aTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGt                                <  1:3707507/93‑1 (MQ=255)
                                                        aTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGt                                <  1:2382502/93‑1 (MQ=255)
                                                             tGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGAt                           >  1:5358367/1‑93 (MQ=255)
                                                             tGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGAt                           <  2:3713157/93‑1 (MQ=255)
                                                              gATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCGGCAACATTAAGGTTTGATa                          <  2:6284482/93‑1 (MQ=255)
                                                                tCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGa                        <  1:1622318/93‑1 (MQ=255)
                                                                 cAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGaa                       <  1:3554724/93‑1 (MQ=255)
                                                                  aGCCCGCGACCGGTGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGaaa                      <  2:1049165/93‑1 (MQ=255)
                                                                   gCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCCACGGCGGGAGCCACCGGCTAAGGCAGCCACATTTAGATTTTGTAGATAc                     >  2:2545899/1‑93 (MQ=255)
                                                                   gCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAAGCAGCAACATTAAAGTTTGATAGAAAc                     >  1:6371795/1‑93 (MQ=255)
                                                                   ccccGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAAc                     <  2:2633416/92‑1 (MQ=255)
                                                                      cgcgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCAAGGCGGAAGGCAGCAACATTAAGGGTTGATAGAAACCGc                  >  1:5311626/1‑93 (MQ=255)
                                                                      cgcgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAAACTAAGGGTTGATAGAAAAggg                  >  2:4864624/1‑92 (MQ=255)
                                                                       gcgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCt                 <  1:6434376/93‑1 (MQ=255)
                                                                         gACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGc               <  1:2933738/93‑1 (MQ=255)
                                                                          aCCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGcc              <  1:17520/93‑1 (MQ=255)
                                                                          aCCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGcc              <  1:3995282/93‑1 (MQ=255)
                                                                          aCCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAAGTTTGATAGAAAAGGCTGcc              >  2:1412710/1‑93 (MQ=255)
                                                                          aCCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAAGCAGCAACATTAAAGGTTGATAGAAAAGGGTGcc              >  2:2569397/1‑93 (MQ=255)
                                                                           ccGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGTAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcg             <  1:2695391/93‑1 (MQ=255)
                                                                           ccGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAAAGGCTGCcg             >  2:3682622/1‑93 (MQ=255)
                                                                           ccGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAACATTAAGGTTTGATAGAAACGGGTGCcg             >  1:1096066/1‑93 (MQ=255)
                                                                            cGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcgc            <  2:3225025/93‑1 (MQ=255)
                                                                            cGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcgc            <  2:4840888/93‑1 (MQ=255)
                                                                             ggggTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcgcg           >  2:549882/1‑93 (MQ=255)
                                                                                gTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACAGGTGGCGCGCAc        >  1:4729361/1‑93 (MQ=255)
                                                                                gTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCGCGGCGTAAGGCAGCAAAATTAAGGTTTGATAGAAACGGCTGgcgcgccc        >  1:4355544/1‑91 (MQ=255)
                                                                                gTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGgcgcgccc        >  1:6082364/1‑91 (MQ=255)
                                                                                gTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCAc        <  2:4344749/93‑1 (MQ=255)
                                                                                    aaCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACCATg    >  1:3350099/1‑93 (MQ=255)
                                                                                     aCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGt   <  2:2833715/93‑1 (MQ=255)
                                                                                     aCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAAATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGt   <  2:2191365/93‑1 (MQ=255)
                                                                                      cAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTa  <  1:264720/93‑1 (MQ=255)
                                                                                          |                                                                                        
ATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTA  >  NC_000913/3588070‑3588248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCC  >  NC_000913/3588067‑3588252
                                                                                             |                                                                                            
AACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGG                                                                                              >  SRR4302537.648817/1‑94 (MQ=60)
  CATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAG                                                                                            <  SRR4302537.1010719/94‑1 (MQ=60)
  CATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAG                                                                                            <  SRR4302537.3697338/94‑1 (MQ=60)
   ATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGA                                                                                           >  SRR4302537.6041512/1‑94 (MQ=60)
   ATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGA                                                                                           <  SRR4302537.297799/94‑1 (MQ=60)
   ATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGA                                                                                           >  SRR4302537.2566537/1‑94 (MQ=60)
    TTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGAT                                                                                          <  SRR4302537.988893/94‑1 (MQ=60)
    TTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGAT                                                                                          <  SRR4302537.2432976/94‑1 (MQ=60)
      GCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCC                                                                                        <  SRR4302537.2882563/94‑1 (MQ=60)
      GCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCC                                                                                        >  SRR4302537.2054739/1‑94 (MQ=60)
       CCTGACAGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCA                                                                                       <  SRR4302537.1267649/94‑1 (MQ=60)
        CTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCAC                                                                                      >  SRR4302537.1193271/1‑94 (MQ=60)
           ACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAAT                                                                                   <  SRR4302537.6283007/94‑1 (MQ=60)
            CCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATA                                                                                  >  SRR4302537.565833/1‑94 (MQ=60)
            CCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATA                                                                                  <  SRR4302537.4336655/94‑1 (MQ=60)
            CCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATA                                                                                  >  SRR4302537.199556/1‑94 (MQ=60)
            CCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGGTAAACAGGGAGATCCACAATA                                                                                  >  SRR4302537.4024245/1‑94 (MQ=60)
             CGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGGTAAACAGGGAGATCCACAATAC                                                                                 >  SRR4302537.2727734/1‑94 (MQ=60)
              GCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACG                                                                                >  SRR4302537.1003760/1‑94 (MQ=60)
              GCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACG                                                                                <  SRR4302537.3869692/94‑1 (MQ=60)
              GCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACG                                                                                <  SRR4302537.3223622/94‑1 (MQ=60)
              GCTGTTTGGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACG                                                                                <  SRR4302537.4457123/94‑1 (MQ=60)
               CTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGG                                                                               >  SRR4302537.6313784/1‑94 (MQ=60)
               CTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGG                                                                               <  SRR4302537.2267165/94‑1 (MQ=60)
                TGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGC                                                                              >  SRR4302537.4875912/1‑94 (MQ=60)
                TGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGC                                                                              >  SRR4302537.3247718/1‑94 (MQ=60)
                TGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGC                                                                              >  SRR4302537.2229606/1‑94 (MQ=60)
                TGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGC                                                                              >  SRR4302537.1636864/1‑94 (MQ=60)
                   TTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGC                                                                           >  SRR4302537.325517/1‑94 (MQ=60)
                    TTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCA                                                                          <  SRR4302537.3613183/94‑1 (MQ=60)
                     TGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAA                                                                         <  SRR4302537.1707954/94‑1 (MQ=60)
                       CGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACG                                                                       <  SRR4302537.5727977/94‑1 (MQ=60)
                       CGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACG                                                                       <  SRR4302537.982658/94‑1 (MQ=60)
                        GCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAgcg                                                                      >  SRR4302537.5523105/1‑91 (MQ=60)
                        GCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGG                                                                      >  SRR4302537.5383492/1‑94 (MQ=60)
                         CGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGC                                                                     <  SRR4302537.1042457/94‑1 (MQ=60)
                         CGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGC                                                                     >  SRR4302537.2556946/1‑94 (MQ=60)
                          GTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGAACCACAATACGGCGGCAACGGCG                                                                    >  SRR4302537.4879072/1‑94 (MQ=60)
                           TGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGG                                                                   <  SRR4302537.1300368/94‑1 (MQ=60)
                            GGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGG                                                                  <  SRR4302537.2066534/94‑1 (MQ=60)
                            GGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGG                                                                  >  SRR4302537.2137045/1‑94 (MQ=60)
                            GGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGG                                                                  <  SRR4302537.1858530/94‑1 (MQ=60)
                            GGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGAGGG                                                                  >  SRR4302537.6485282/1‑94 (MQ=60)
                             GTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGA                                                                 <  SRR4302537.31974/94‑1 (MQ=60)
                             GTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGA                                                                 >  SRR4302537.2489645/1‑94 (MQ=60)
                               TCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGAAACGGCGGGggg                                                               >  SRR4302537.5643769/1‑91 (MQ=60)
                                 CAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGgcc                                                             >  SRR4302537.4059412/1‑91 (MQ=60)
                                 CAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCA                                                             <  SRR4302537.1724811/94‑1 (MQ=60)
                                  AGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCAC                                                            >  SRR4302537.3378348/1‑94 (MQ=60)
                                  AGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCAC                                                            <  SRR4302537.2669519/94‑1 (MQ=60)
                                  AGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCAC                                                            >  SRR4302537.3849907/1‑94 (MQ=60)
                                  AGTCGTAGCCGAACTCCGCGAGAAAATGGGAGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACCGCGGGAGgccc                                                            >  SRR4302537.3068500/1‑90 (MQ=60)
                                   GTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACG                                                           <  SRR4302537.3866156/94‑1 (MQ=60)
                                   GTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACG                                                           <  SRR4302537.6032499/94‑1 (MQ=60)
                                   GTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACG                                                           <  SRR4302537.6120107/94‑1 (MQ=60)
                                    TCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGG                                                          >  SRR4302537.2236871/1‑94 (MQ=60)
                                    TCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGG                                                          <  SRR4302537.4210613/94‑1 (MQ=60)
                                     CGTAGCCTACCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGC                                                         <  SRR4302537.174936/94‑1 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGcgcccccac                                                         >  SRR4302537.2534933/1‑85 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGC                                                         <  SRR4302537.1422571/94‑1 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGC                                                         <  SRR4302537.5191063/94‑1 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGC                                                         <  SRR4302537.3837503/94‑1 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGC                                                         <  SRR4302537.6445469/94‑1 (MQ=60)
                                     CGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGC                                                         >  SRR4302537.1422306/1‑94 (MQ=60)
                                      GTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCG                                                        <  SRR4302537.2401251/94‑1 (MQ=60)
                                        AGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGA                                                      >  SRR4302537.3676549/1‑94 (MQ=60)
                                        AGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGGA                                                      >  SRR4302537.3265968/1‑94 (MQ=60)
                                         GCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGagccggaggaa                                                     >  SRR4302537.1390038/1‑83 (MQ=60)
                                          CCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCGGCGcgag                                                    >  SRR4302537.254860/1‑90 (MQ=60)
                                          CCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGTAAG                                                    >  SRR4302537.2876996/1‑94 (MQ=60)
                                           CGAACTCCGCGATAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGG                                                   <  SRR4302537.699080/94‑1 (MQ=60)
                                           CGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGgggcccggcggaagg                                                   >  SRR4302537.5420631/1‑79 (MQ=60)
                                           CGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGGCACGGCGTAAGG                                                   >  SRR4302537.6337832/1‑94 (MQ=60)
                                           CGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGG                                                   <  SRR4302537.3452867/94‑1 (MQ=60)
                                           CGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGG                                                   <  SRR4302537.1691384/94‑1 (MQ=60)
                                            GAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGC                                                  <  SRR4302537.402329/94‑1 (MQ=60)
                                             ACCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCA                                                 <  SRR4302537.206843/94‑1 (MQ=60)
                                             AACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGgggcacgggcgaaggga                                                 >  SRR4302537.5627645/1‑77 (MQ=60)
                                             AACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCGGCGTAAGGCA                                                 >  SRR4302537.716803/1‑94 (MQ=60)
                                              ACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAgggggggggccacgcggtaagggag                                                >  SRR4302537.3949653/1‑69 (MQ=60)
                                              ACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAG                                                <  SRR4302537.5901643/94‑1 (MQ=60)
                                              ACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAG                                                <  SRR4302537.2914561/94‑1 (MQ=60)
                                              ACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAG                                                <  SRR4302537.2730956/94‑1 (MQ=60)
                                              ACTCCGCGAGAAAAGGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAG                                                <  SRR4302537.1307164/94‑1 (MQ=60)
                                               CTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGGCACGGCGTAAGGGAGC                                               >  SRR4302537.665813/1‑94 (MQ=60)
                                               CTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCGGCGGAAGGCAGC                                               >  SRR4302537.3842217/1‑94 (MQ=60)
                                               CTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGC                                               >  SRR4302537.2879803/1‑94 (MQ=60)
                                               CTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGC                                               <  SRR4302537.5371554/94‑1 (MQ=60)
                                                 CCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCCCGGCGGAAGGGAGCAA                                             >  SRR4302537.5868713/1‑94 (MQ=60)
                                                     GAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATT                                         <  SRR4302537.4210828/94‑1 (MQ=60)
                                                     GAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATT                                         >  SRR4302537.1641754/1‑94 (MQ=60)
                                                     GAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATT                                         <  SRR4302537.7779/94‑1 (MQ=60)
                                                      AGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGgggcccgggcgaaagcagcaacatta                                        >  SRR4302537.5986594/1‑68 (MQ=60)
                                                      AGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTA                                        <  SRR4302537.3129028/94‑1 (MQ=60)
                                                      AGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGTGAGGCAGCAACAATA                                        >  SRR4302537.106283/1‑94 (MQ=60)
                                                       GAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGGAAGGCAGCAACATTAA                                       >  SRR4302537.1360436/1‑94 (MQ=60)
                                                       GAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGgccccgcgtaaaggagcaacattta                                       >  SRR4302537.129022/1‑69 (MQ=60)
                                                       GAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAA                                       <  SRR4302537.1368893/94‑1 (MQ=60)
                                                        AAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCTACGGCGTAAGGCAGCAACATTAAG                                      <  SRR4302537.87967/94‑1 (MQ=60)
                                                        AAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAG                                      >  SRR4302537.3462488/1‑94 (MQ=60)
                                                        AAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAG                                      <  SRR4302537.4969765/94‑1 (MQ=60)
                                                        AAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAG                                      <  SRR4302537.1068146/94‑1 (MQ=60)
                                                         AAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGG                                     <  SRR4302537.3169894/94‑1 (MQ=60)
                                                          AATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGT                                    <  SRR4302537.4619432/94‑1 (MQ=60)
                                                          AATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGT                                    <  SRR4302537.1043767/94‑1 (MQ=60)
                                                           ATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTT                                   <  SRR4302537.3733578/94‑1 (MQ=60)
                                                           ATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTT                                   >  SRR4302537.2012365/1‑94 (MQ=60)
                                                           ATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTT                                   <  SRR4302537.2399290/94‑1 (MQ=60)
                                                            TGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATCCGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTT                                  <  SRR4302537.3941812/94‑1 (MQ=60)
                                                             GGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTG                                 <  SRR4302537.5574821/94‑1 (MQ=60)
                                                               GTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGAT                               >  SRR4302537.5395062/1‑94 (MQ=60)
                                                               GTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGGTTGAT                               >  SRR4302537.922126/1‑94 (MQ=60)
                                                                TGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGTAAGGGAGCAACATTAAGGGTTGATA                              >  SRR4302537.2944002/1‑94 (MQ=60)
                                                                TGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCCCCGCGTAAGGCgccaatatttagagttgata                              >  SRR4302537.4950579/1‑74 (MQ=60)
                                                                 GATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGgaccagggcggaagggagcaacactcagatgtggtaa                             >  SRR4302537.6007035/1‑57 (MQ=60)
                                                                 GATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAG                             <  SRR4302537.4841208/94‑1 (MQ=60)
                                                                  ATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGA                            >  SRR4302537.5304224/1‑94 (MQ=60)
                                                                   TCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAA                           <  SRR4302537.1633666/94‑1 (MQ=60)
                                                                    CAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAA                          <  SRR4302537.3579818/94‑1 (MQ=60)
                                                                    CAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAA                          <  SRR4302537.2531765/94‑1 (MQ=60)
                                                                     AGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAAGCAGCAACATTAAAGTTTGATAGAAAC                         >  SRR4302537.6415249/1‑94 (MQ=60)
                                                                      GCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACG                        <  SRR4302537.765849/94‑1 (MQ=60)
                                                                      GCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACG                        <  SRR4302537.2383126/94‑1 (MQ=60)
                                                                      GCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACG                        <  SRR4302537.5531211/94‑1 (MQ=60)
                                                                      GCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAAGTTTGATAGAAACG                        >  SRR4302537.5302358/1‑94 (MQ=60)
                                                                       CCCGCGACCGGGGTTAAATAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGAGTAAGGCAGCAACATTAAGGTTTGATAGAAACGG                       <  SRR4302537.5127442/94‑1 (MQ=60)
                                                                        CCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCAAGGCGGAAGGCAGCAACATTAAGGGTTGATAGAAACCGC                      >  SRR4302537.5348110/1‑94 (MQ=60)
                                                                          GCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTG                    <  SRR4302537.6478071/94‑1 (MQ=60)
                                                                           CGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGCGCCACGGCGGAAGGCAGCAAAATTAAGGTTTGATAGAAAAGGCTGC                   >  SRR4302537.1064281/1‑94 (MQ=60)
                                                                            GACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGTAAGGCAGCAACATTAAAGTTTTATAGAAAagggtgtc                  >  SRR4302537.868083/1‑86 (MQ=60)
                                                                            GACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCC                  <  SRR4302537.2954339/94‑1 (MQ=60)
                                                                             ACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCG                 <  SRR4302537.17659/94‑1 (MQ=60)
                                                                             ACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCG                 <  SRR4302537.4023247/94‑1 (MQ=60)
                                                                             ACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAACATTAAGGTTTGATAGAAACGGGTGCCG                 >  SRR4302537.1103639/1‑94 (MQ=60)
                                                                              CCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGTAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGC                <  SRR4302537.2714224/94‑1 (MQ=60)
                                                                                  GGTTAAACAGGGATATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCAC            >  SRR4302537.1824249/1‑94 (MQ=60)
                                                                                  GGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACAGGTGGCGCGCAC            >  SRR4302537.4762044/1‑94 (MQ=60)
                                                                                  GGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCGCGGCGTAAGGCAGCAAAATTAAGGTTTGATAGAAACGGCTGGCGCGCCC            >  SRR4302537.4385698/1‑94 (MQ=60)
                                                                                  GGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGGCGCGCCC            >  SRR4302537.6124152/1‑94 (MQ=60)
                                                                                  GGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAAAGGCTGCCGCGCAC            >  SRR4302537.5650431/1‑94 (MQ=60)
                                                                                      AAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAGGCAGCAACATTAAGGGTTGATAGAAAAGGCTGCCGCGCACGATG        >  SRR4302537.3436775/1‑94 (MQ=60)
                                                                                      AAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACCATG        >  SRR4302537.3373816/1‑94 (MQ=60)
                                                                                         CAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTAC     <  SRR4302537.266580/94‑1 (MQ=60)
                                                                                         CAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTAC     <  SRR4302537.6180461/94‑1 (MQ=60)
                                                                                          AGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGGTTGATAGAAACGGCTGCCGCGCACGATGTACT    >  SRR4302537.5698892/1‑94 (MQ=60)
                                                                                            GGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCC  <  SRR4302537.4617187/94‑1 (MQ=60)
                                                                                            GGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCC  <  SRR4302537.5072209/94‑1 (MQ=60)
                                                                                             |                                                                                            
AACATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACTCC  >  NC_000913/3588067‑3588252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑