Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,677 +G intergenic (+175/‑264) kup → / → rbsD low affinity potassium transport system/RbsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,6761.G100.0% 885.5 / NA 252intergenic (+174/‑265)kup/rbsDlow affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (138/114);  total (138/114)

Only 100 of 282 total aligned reads displayed.

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA  >  NC_000913/3930584‑3930763
                                                                                              |                                                                                        
gTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑c                                                                                           <  2:1358436/93‑1 (MQ=255)
 tAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑g                                                                                         >  1:4623277/1‑93 (MQ=255)
 tAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑g                                                                                         <  1:3527707/93‑1 (MQ=255)
  agaagaAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑gg                                                                                        <  1:313177/93‑2 (MQ=255)
   gaagaaGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggc                                                                                       >  1:2252266/1‑91 (MQ=255)
    aagaagTTTTCCACCGCTGCGGTCTGGACGGATTAAGTCGCCGGATGATGGAAACCTCATTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggca                                                                                      >  2:1456450/1‑90 (MQ=255)
     agaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggcaa                                                                                     >  1:933961/1‑89 (MQ=255)
     agaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggcaa                                                                                     >  1:491874/1‑89 (MQ=255)
     agaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑ggcaa                                                                                     >  1:4378620/1‑89 (MQ=255)
       aagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAAc                                                                                   <  2:220469/93‑1 (MQ=255)
          ttttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGcc                                                                                <  1:4147074/93‑1 (MQ=255)
          ttttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGcc                                                                                <  2:1272230/93‑1 (MQ=255)
          ttttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGcc                                                                                >  1:5069948/1‑93 (MQ=255)
          ttttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGcc                                                                                >  1:3200753/1‑93 (MQ=255)
              ccACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTg                                                                            <  2:6385280/93‑1 (MQ=255)
               cACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTgg                                                                           <  2:2277845/93‑1 (MQ=255)
                aCCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGt                                                                          <  2:4794724/93‑1 (MQ=255)
                  cGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAt                                                                        <  2:4145219/93‑1 (MQ=255)
                  cGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAt                                                                        <  2:552647/93‑1 (MQ=255)
                  cGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAACTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAt                                                                        >  2:5267875/1‑93 (MQ=255)
                   gCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAtt                                                                       <  1:2887730/93‑1 (MQ=255)
                    cTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTAttt                                                                      >  2:6001720/1‑93 (MQ=255)
                     tGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTg                                                                     <  1:2560606/93‑1 (MQ=255)
                     tGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTg                                                                     <  2:4292776/93‑1 (MQ=255)
                       cGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCg                                                                   <  2:4522135/93‑1 (MQ=255)
                        ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGt                                                                  <  2:3520487/93‑1 (MQ=255)
                         gTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTc                                                                 >  2:302420/1‑93 (MQ=255)
                         gTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTc                                                                 >  1:2192064/1‑93 (MQ=255)
                          tCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCt                                                                >  1:2243198/1‑93 (MQ=255)
                          tCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCt                                                                <  2:532439/93‑1 (MQ=255)
                           cTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTg                                                               <  2:1082653/93‑1 (MQ=255)
                            tGGAAGGATTAAGTTGCCGGATGGTGGAAACCTCCTTCTTTATGACGCATGAGTCGCTGATCCTAG‑G‑CAAACGCCGGTGGTACTTGGGtacgc                                                              >  2:1020487/1‑89 (MQ=255)
                            tGGAAGCATTAAGTGGCCGCATAATGCAAACCTCCTTCTTTATGTCGCATGAGTCGTCGATCCT‑C‑GACAAAAGCCCGTGGTATTCGCGTATgc                                                              <  2:4416036/93‑1 (MQ=255)
                             ggAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgcg                                                             <  2:1149375/93‑1 (MQ=255)
                             ggAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTgcg                                                             <  2:4933502/93‑1 (MQ=255)
                                  gATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCa                                                        >  1:5026156/1‑93 (MQ=255)
                                  gATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCa                                                        >  2:5639719/1‑93 (MQ=255)
                                  gATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCa                                                        >  2:5697058/1‑93 (MQ=255)
                                   aTTAAGTTGCCTGATGATGGAAGCTTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑GCCAAACGCCCGTGGTATTTGCGTCTGCGCGGCaa                                                       <  2:3681333/93‑1 (MQ=255)
                                    ttAAGTTGCCGGATGATGGAAACCTCTTTTTTTATGTCGCATGAGGCGATGATCCT‑CGG‑GAAAAGCCCTTGGTATTTGCGTCTGCGCAGGAAg                                                      >  2:5042573/1‑93 (MQ=255)
                                    ttAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAg                                                      <  1:4131969/93‑1 (MQ=255)
                                     tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGc                                                     <  2:843659/93‑1 (MQ=255)
                                     tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGc                                                     <  1:4532424/93‑1 (MQ=255)
                                     tAAGTCGCCGTATGATGGAAGCCTCCTTCTTTATCTCGCATGAGCCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGc                                                     <  2:2117797/93‑1 (MQ=255)
                                      aaGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCt                                                    >  1:2990060/1‑93 (MQ=255)
                                       aGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTg                                                   >  1:3914854/1‑93 (MQ=255)
                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                  <  1:1671003/93‑1 (MQ=255)
                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                  >  2:421030/1‑93 (MQ=255)
                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                  >  1:762799/1‑93 (MQ=255)
                                         ttGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTa                                                 >  1:5973851/1‑93 (MQ=255)
                                          tGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                                                <  2:2032619/93‑1 (MQ=255)
                                          tGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                                                >  1:2478356/1‑93 (MQ=255)
                                            ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                                              <  2:2272882/93‑1 (MQ=255)
                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                            >  2:3376985/1‑93 (MQ=255)
                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         >  1:6367333/1‑93 (MQ=255)
                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         <  2:373040/93‑1 (MQ=255)
                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         <  2:4538892/93‑1 (MQ=255)
                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                         >  1:1017984/1‑93 (MQ=255)
                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                      >  2:5823829/1‑93 (MQ=255)
                                                     ggAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCa                                     >  1:277467/1‑93 (MQ=255)
                                                      gAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCaa                                    <  1:5707429/93‑1 (MQ=255)
                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                  >  1:6174583/1‑93 (MQ=255)
                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                  >  2:5208447/1‑93 (MQ=255)
                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                 >  1:144157/1‑93 (MQ=255)
                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGtg                                >  1:5161309/1‑92 (MQ=255)
                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                                >  1:2037536/1‑93 (MQ=255)
                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                                >  1:5561109/1‑93 (MQ=255)
                                                              cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc                            >  2:2534866/1‑93 (MQ=255)
                                                              cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc                            <  1:1766345/93‑1 (MQ=255)
                                                              cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc                            >  1:2723487/1‑93 (MQ=255)
                                                              cttcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc                            >  2:3274123/1‑93 (MQ=255)
                                                                tcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          <  1:3345995/93‑1 (MQ=255)
                                                                tcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          >  2:5849457/1‑93 (MQ=255)
                                                                tcttTATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          >  2:1904351/1‑93 (MQ=255)
                                                                tcttTATGTCGCATGAGTCGGTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                          >  1:1816108/1‑93 (MQ=255)
                                                                   ttATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc                       >  1:2367565/1‑93 (MQ=255)
                                                                   ttATGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc                       <  2:4663205/93‑1 (MQ=255)
                                                                     aTGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGt                     <  2:5297115/93‑1 (MQ=255)
                                                                      tGTCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTg                    >  1:6074264/1‑93 (MQ=255)
                                                                        tCGCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCa                  >  2:5610771/1‑93 (MQ=255)
                                                                          gCATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAcc                <  1:2151573/93‑1 (MQ=255)
                                                                          gCATGAGTAGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAcc                <  2:6409497/93‑1 (MQ=255)
                                                                           cATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCa               <  2:888659/93‑1 (MQ=255)
                                                                           cATGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCa               >  2:2781943/1‑93 (MQ=255)
                                                                             tGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa             <  2:3900776/93‑1 (MQ=255)
                                                                             tGAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa             >  1:2812108/1‑93 (MQ=255)
                                                                              gAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAt            <  2:754682/93‑1 (MQ=255)
                                                                              gAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAt            <  2:1721632/93‑1 (MQ=255)
                                                                              gAGTCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAt            <  2:1415512/93‑1 (MQ=255)
                                                                                 tCGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCaa         >  2:4009833/1‑93 (MQ=255)
                                                                                  cGTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAt        >  2:3614425/1‑93 (MQ=255)
                                                                                   gTTGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt       <  1:1148884/93‑1 (MQ=255)
                                                                                   gTTGAGCCT‑CGG‑CAAACGCTCGTTGTATTTGCGTCTGCGCGGCAAGCTGTCCTTGCTGCTGTAACGTAATGCGCTGCGTGCACCAGATCAAtt       <  2:2967335/93‑1 (MQ=255)
                                                                                    ttGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt      >  2:1664221/1‑93 (MQ=255)
                                                                                    ttGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt      >  2:6434162/1‑93 (MQ=255)
                                                                                    ttGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt      >  2:3547190/1‑93 (MQ=255)
                                                                                     tGATCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTg     <  1:324330/93‑1 (MQ=255)
                                                                                       aTCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaa   <  2:2420713/93‑1 (MQ=255)
                                                                                       aTCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaa   >  1:191452/1‑93 (MQ=255)
                                                                                        tCCT‑CGG‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaaa  <  1:1219084/93‑1 (MQ=255)
                                                                                              |                                                                                        
GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCT‑C‑G‑CAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA  >  NC_000913/3930584‑3930763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  >  NC_000913/3930584‑3930770
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GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               >  SRR4302537.4655276/1‑94 (MQ=60)
GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               >  SRR4302537.5117087/1‑94 (MQ=60)
 TAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GG                                                                                              <  SRR4302537.3552657/94‑1 (MQ=60)
 TAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GG                                                                                              >  SRR4302537.4672386/1‑94 (MQ=60)
 TAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GG                                                                                              <  SRR4302537.2975722/94‑1 (MQ=60)
 TAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GG                                                                                              <  SRR4302537.2262040/94‑1 (MQ=60)
  AGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggc                                                                                             >  SRR4302537.2268165/1‑92 (MQ=60)
  AGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggc                                                                                             <  SRR4302537.315343/94‑3 (MQ=60)
   GAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggca                                                                                            >  SRR4302537.5493955/1‑91 (MQ=60)
    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302537.4408847/1‑94 (MQ=60)
    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302537.495257/1‑94 (MQ=60)
    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302537.940395/1‑94 (MQ=60)
     AGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA                                                                                           <  SRR4302537.2317004/94‑1 (MQ=60)
     AGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA                                                                                           >  SRR4302537.5871427/1‑94 (MQ=60)
         GTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCC                                                                                       >  SRR4302537.3223254/1‑94 (MQ=60)
         GTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCC                                                                                       >  SRR4302537.5104849/1‑94 (MQ=60)
          TTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCC                                                                                      <  SRR4302537.4176014/94‑1 (MQ=60)
          TTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCC                                                                                      <  SRR4302537.2797618/94‑1 (MQ=60)
            TTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGT                                                                                    >  SRR4302537.4374344/1‑94 (MQ=60)
            TTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGT                                                                                    >  SRR4302537.4174031/1‑94 (MQ=60)
            TTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGT                                                                                    >  SRR4302537.5713992/1‑94 (MQ=60)
              CCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGG                                                                                  >  SRR4302537.300466/1‑94 (MQ=60)
              CCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGG                                                                                  >  SRR4302537.1887752/1‑94 (MQ=60)
              CCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGG                                                                                  >  SRR4302537.4036073/1‑94 (MQ=60)
              CCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGG                                                                                  >  SRR4302537.6179019/1‑94 (MQ=60)
               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAACTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGT                                                                                 >  SRR4302537.1691842/1‑94 (MQ=60)
                   GCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTT                                                                             <  SRR4302537.2907997/94‑1 (MQ=60)
                   GCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTT                                                                             >  SRR4302537.884588/1‑94 (MQ=60)
                   GCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTT                                                                             <  SRR4302537.2907809/94‑1 (MQ=60)
                    CTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTG                                                                            <  SRR4302537.2182783/94‑1 (MQ=60)
                    CTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTG                                                                            <  SRR4302537.3664397/94‑1 (MQ=60)
                     TGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGC                                                                           <  SRR4302537.2578548/94‑1 (MQ=60)
                      GCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCG                                                                          <  SRR4302537.2763262/94‑1 (MQ=60)
                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTC                                                                        >  SRR4302537.2207506/1‑94 (MQ=60)
                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTC                                                                        >  SRR4302537.3291213/1‑94 (MQ=60)
                         GTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCT                                                                       >  SRR4302537.2259018/1‑94 (MQ=60)
                         GTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCT                                                                       <  SRR4302537.5653364/94‑1 (MQ=60)
                          TCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTG                                                                      <  SRR4302537.1603653/94‑1 (MQ=60)
                            TGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCG                                                                    <  SRR4302537.4499527/94‑1 (MQ=60)
                            TAGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCG                                                                    >  SRR4302537.4760638/1‑94 (MQ=60)
                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGC                                                                   >  SRR4302537.3694984/1‑94 (MQ=60)
                              GAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCG                                                                  <  SRR4302537.2177301/94‑1 (MQ=60)
                              GAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCG                                                                  >  SRR4302537.1199999/1‑94 (MQ=60)
                              GAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCG                                                                  <  SRR4302537.1110998/94‑1 (MQ=60)
                                 GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCA                                                               >  SRR4302537.5060769/1‑94 (MQ=60)
                                  GATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAA                                                              >  SRR4302537.3985244/1‑94 (MQ=60)
                                   ATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                             <  SRR4302537.3492638/94‑1 (MQ=60)
                                   ATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                             <  SRR4302537.952356/94‑1 (MQ=60)
                                   ATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                             >  SRR4302537.1943672/1‑94 (MQ=60)
                                   ATTAAGTTGCCGGATGATGGAAACCTCCTTCGTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAG                                                             <  SRR4302537.3187648/94‑1 (MQ=60)
                                    TTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGC                                                            <  SRR4302537.4614295/94‑1 (MQ=60)
                                    TTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGC                                                            >  SRR4302537.2110429/1‑94 (MQ=60)
                                    TTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGC                                                            <  SRR4302537.4160805/94‑1 (MQ=60)
                                     TAAGTTGCCGGATGATGGAACCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCT                                                           <  SRR4302537.5420614/94‑1 (MQ=60)
                                     TAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCT                                                           <  SRR4302537.4563811/94‑1 (MQ=60)
                                     TAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCT                                                           >  SRR4302537.3010936/1‑94 (MQ=60)
                                      AAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTG                                                          >  SRR4302537.3942219/1‑94 (MQ=60)
                                      AAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTG                                                          >  SRR4302537.179607/1‑94 (MQ=60)
                                      AAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTG                                                          >  SRR4302537.136722/1‑94 (MQ=60)
                                       AGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGT                                                         >  SRR4302537.768012/1‑94 (MQ=60)
                                        GTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTA                                                        >  SRR4302537.5129374/1‑94 (MQ=60)
                                        GTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTA                                                        <  SRR4302537.1682706/94‑1 (MQ=60)
                                        GTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTA                                                        >  SRR4302537.6014858/1‑94 (MQ=60)
                                         TTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAC                                                       >  SRR4302537.2495780/1‑94 (MQ=60)
                                         TTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAC                                                       >  SRR4302537.3374609/1‑94 (MQ=60)
                                           gacgaATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTT                                                     <  SRR4302537.435603/89‑1 (MQ=60)
                                           GCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTT                                                     >  SRR4302537.1519281/1‑94 (MQ=60)
                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                    <  SRR4302537.2519459/94‑1 (MQ=60)
                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                    <  SRR4302537.1231566/94‑1 (MQ=60)
                                             CGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGC                                                   <  SRR4302537.6285138/94‑1 (MQ=60)
                                              GGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCT                                                  >  SRR4302537.5306075/1‑94 (MQ=60)
                                               GATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTG                                                 <  SRR4302537.2370281/94‑1 (MQ=60)
                                                ATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGC                                                >  SRR4302537.1024997/1‑94 (MQ=60)
                                                ATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGC                                                >  SRR4302537.6410759/1‑94 (MQ=60)
                                                 TGATGTAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCT                                               <  SRR4302537.5882619/94‑1 (MQ=60)
                                                    TGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCA                                            >  SRR4302537.279408/1‑94 (MQ=60)
                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          <  SRR4302537.89760/94‑1 (MQ=60)
                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          <  SRR4302537.2037577/94‑1 (MQ=60)
                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          <  SRR4302537.5746487/94‑1 (MQ=60)
                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         <  SRR4302537.496203/94‑1 (MQ=60)
                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         >  SRR4302537.6217110/1‑94 (MQ=60)
                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         >  SRR4302537.6217109/1‑94 (MQ=60)
                                                        AACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGT                                        >  SRR4302537.145185/1‑94 (MQ=60)
                                                         ACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTG                                       >  SRR4302537.5196892/1‑94 (MQ=60)
                                                         ACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTA                                       >  SRR4302537.3262318/1‑94 (MQ=60)
                                                         ACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTA                                       >  SRR4302537.2051742/1‑94 (MQ=60)
                                                         ACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTA                                       >  SRR4302537.5599111/1‑94 (MQ=60)
                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAACCTGTACTTGCTGCTGCAACGTAAT                                     >  SRR4302537.5810129/1‑94 (MQ=60)
                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                    <  SRR4302537.3352339/94‑1 (MQ=60)
                                                             CCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGC                                   >  SRR4302537.2742509/1‑94 (MQ=60)
                                                             CCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGC                                   >  SRR4302537.2801987/1‑94 (MQ=60)
                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                  >  SRR4302537.168386/1‑94 (MQ=60)
                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                  >  SRR4302537.2237048/1‑94 (MQ=60)
                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                  <  SRR4302537.1778644/94‑1 (MQ=60)
                                                               TTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGC                                 >  SRR4302537.4985902/1‑94 (MQ=60)
                                                               TTCTTTATGTCGCATGAGTCGGTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGC                                 >  SRR4302537.1828768/1‑94 (MQ=60)
                                                                TCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCT                                >  SRR4302537.4631535/1‑94 (MQ=60)
                                                                TCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCT                                <  SRR4302537.3369682/94‑1 (MQ=60)
                                                                  TTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGC                              <  SRR4302537.1212283/94‑1 (MQ=60)
                                                                  TTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGC                              >  SRR4302537.2384253/1‑94 (MQ=60)
                                                                    TATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGT                            <  SRR4302537.427219/94‑1 (MQ=60)
                                                                     ATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG                           >  SRR4302537.6116006/1‑94 (MQ=60)
                                                                     ATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG                           >  SRR4302537.508282/1‑94 (MQ=60)
                                                                     ATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG                           >  SRR4302537.3171439/1‑94 (MQ=60)
                                                                     ATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTG                           <  SRR4302537.3144353/94‑1 (MQ=60)
                                                                       GTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCA                         >  SRR4302537.2248322/1‑94 (MQ=60)
                                                                       GTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCA                         >  SRR4302537.3013165/1‑94 (MQ=60)
                                                                       GTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCA                         <  SRR4302537.4506331/94‑1 (MQ=60)
                                                                         CGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACC                       >  SRR4302537.210900/1‑94 (MQ=60)
                                                                          GCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCA                      <  SRR4302537.2166739/94‑1 (MQ=60)
                                                                           CATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAG                     >  SRR4302537.2441261/1‑94 (MQ=60)
                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    >  SRR4302537.2831773/1‑94 (MQ=60)
                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    >  SRR4302537.2872072/1‑94 (MQ=60)
                                                                             TGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAT                   >  SRR4302537.717229/1‑94 (MQ=60)
                                                                             AGAGTCGTTGATACTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAT                   <  SRR4302537.6032355/94‑1 (MQ=60)
                                                                               AGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCA                 >  SRR4302537.2689710/1‑94 (MQ=60)
                                                                               AGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCA                 >  SRR4302537.256108/1‑94 (MQ=60)
                                                                                GTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAA                <  SRR4302537.713575/94‑1 (MQ=60)
                                                                                 TCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAT               >  SRR4302537.2611030/1‑94 (MQ=60)
                                                                                   GTTGATCCTCGGCAAACGCCCGTGTTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             <  SRR4302537.1899546/94‑1 (MQ=60)
                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             <  SRR4302537.2406894/94‑1 (MQ=60)
                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             <  SRR4302537.1156872/94‑1 (MQ=60)
                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             >  SRR4302537.6151777/1‑94 (MQ=60)
                                                                                     TGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGA           <  SRR4302537.326579/94‑1 (MQ=60)
                                                                                      GATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA          >  SRR4302537.192813/1‑94 (MQ=60)
                                                                                        TCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAT        <  SRR4302537.1227564/94‑1 (MQ=60)
                                                                                         CCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATC       <  SRR4302537.6099751/94‑1 (MQ=60)
                                                                                          CTCGGCAAACGCCCGTGGTATTTGTGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC      <  SRR4302537.3439448/94‑1 (MQ=60)
                                                                                            cgGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG    <  SRR4302537.3727846/92‑1 (MQ=60)
                                                                                            cgGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG    <  SRR4302537.3269540/92‑1 (MQ=60)
                                                                                            cgGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG    <  SRR4302537.1850801/92‑1 (MQ=60)
                                                                                            G‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC   <  SRR4302537.3791620/94‑1 (MQ=60)
                                                                                            G‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC   <  SRR4302537.1492588/94‑1 (MQ=60)
                                                                                              GAAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  <  SRR4302537.746224/94‑1 (MQ=60)
                                                                                              |                                                                                             
GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  >  NC_000913/3930584‑3930770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑