Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,458,811	C→T	A51V (GCG→GTG)	hofG →	putative general protein secretion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,458,811	0	C	T	99.3%	1010.6 / NA	290	A51V (GCG→GTG)	hofG	putative general protein secretion protein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (147/140); minor base G (2/0); total (150/140)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGA  >  NC_000913/3458721‑3458900

ttATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGgtgc                                                                                         >  2:8704484/1‑93 (MQ=255)

ttATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGgtgc                                                                                         <  1:572315/93‑1 (MQ=255)

ttATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGgtgc                                                                                         <  2:1099054/93‑1 (MQ=255)

   tCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTgg                                                                                      <  1:5508075/93‑1 (MQ=255)

      gCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGaaa                                                                                   >  1:2477841/1‑93 (MQ=255)

       cGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGaaaa                                                                                  <  2:1932001/93‑1 (MQ=255)

        gTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAc                                                                                 >  1:1456383/1‑93 (MQ=255)

        gTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAc                                                                                 >  2:6223324/1‑93 (MQ=255)

        gTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAc                                                                                 >  1:909046/1‑93 (MQ=255)

        gTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAc                                                                                 >  1:3211270/1‑93 (MQ=255)

         tatttGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACg                                                                                >  2:7825034/4‑93 (MQ=255)

         tACTTGCCAGTCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACg                                                                                >  1:1218076/1‑93 (MQ=255)

         tACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACg                                                                                >  2:6105156/1‑93 (MQ=255)

         tACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACg                                                                                <  1:926850/93‑1 (MQ=255)

          aCTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGc                                                                               >  2:1711352/1‑93 (MQ=255)

            ttGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGccc                                                                             <  1:5365632/93‑1 (MQ=255)

            ttGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGccc                                                                             <  1:3111219/93‑1 (MQ=255)

             tGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCt                                                                            >  1:6165218/1‑93 (MQ=255)

               ccAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTg                                                                          <  2:6753421/93‑1 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                        <  2:4145229/93‑1 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                        <  2:5251384/93‑1 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                        >  2:6514540/1‑93 (MQ=255)

                 aGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGat                                                                        >  2:1262190/1‑93 (MQ=255)

                  gCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGata                                                                       >  1:1611176/1‑93 (MQ=255)

                  gCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGata                                                                       >  2:6470323/1‑93 (MQ=255)

                  gCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGata                                                                       >  2:264463/1‑93 (MQ=255)

                   ccTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGatat                                                                      <  1:5856238/93‑1 (MQ=255)

                    cTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATg                                                                     >  2:8798345/1‑93 (MQ=255)

                     tggtggTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGt                                                                    <  1:3597528/93‑1 (MQ=255)

                      ggtggtTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTa                                                                   >  2:3506765/1‑93 (MQ=255)

                       gtggtTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTAc                                                                  >  2:762283/1‑93 (MQ=255)

                        tggtTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACa                                                                 <  2:2106661/93‑1 (MQ=255)

                        tggtTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACa                                                                 <  2:3689836/93‑1 (MQ=255)

                           ttCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAAc                                                              >  2:3866069/1‑93 (MQ=255)

                            tCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACt                                                             >  2:5510149/1‑93 (MQ=255)

                            tCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACt                                                             >  1:1503137/1‑93 (MQ=255)

                             cctaacctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTc                                                            <  2:593989/93‑1 (MQ=255)

                             cctaacctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTc                                                            >  2:188896/1‑93 (MQ=255)

                               taacctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGa                                                          <  2:6343930/93‑1 (MQ=255)

                                 acctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACa                                                        >  2:3350683/1‑93 (MQ=255)

                                 acctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACa                                                        >  2:6924773/1‑93 (MQ=255)

                                 acctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACa                                                        >  2:4956180/1‑93 (MQ=255)

                                  cctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACaa                                                       <  1:3900126/93‑1 (MQ=255)

                                   ctaaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                                      >  2:311109/1‑93 (MQ=255)

                                   ctaaTGGACAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAat                                                      <  2:4901069/93‑1 (MQ=255)

                                     aaTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatca                                                    <  2:5314608/93‑1 (MQ=255)

                                      aTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatcat                                                   <  1:8557760/93‑1 (MQ=255)

                                      aTGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatcat                                                   <  2:7685013/93‑1 (MQ=255)

                                       tGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAatcatc                                                  <  1:696693/93‑1 (MQ=255)

                                        gggCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                                                 >  2:5388121/1‑93 (MQ=255)

                                        gggCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAtcatca                                                 <  1:1040911/93‑1 (MQ=255)

                                         ggCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAc                                                <  2:4664038/93‑1 (MQ=255)

                                         ggCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAc                                                >  1:6763911/1‑93 (MQ=255)

                                          gCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACt                                               >  1:7774180/1‑93 (MQ=255)

                                          gCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCGTGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACt                                               <  2:7519901/93‑1 (MQ=255)

                                           cAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTa                                              <  1:5268539/93‑1 (MQ=255)

                                            aaTAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAt                                             >  2:605880/1‑93 (MQ=255)

                                             aTAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATc                                            >  1:407959/1‑93 (MQ=255)

                                              tAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATcc                                           <  2:626886/93‑1 (MQ=255)

                                               aaaGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACACACTCGACAATCATCACTATCCa                                          >  2:1169240/1‑93 (MQ=255)

                                                 aGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAAc                                        <  2:5518821/93‑1 (MQ=255)

                                                 aGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAAc                                        <  1:3638770/93‑1 (MQ=255)

                                                  gAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATCTTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAAcc                                       >  1:1816520/1‑93 (MQ=255)

                                                  gAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAAcc                                       >  1:1729925/1‑93 (MQ=255)

                                                   aaaaGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACca                                      >  2:6635894/1‑93 (MQ=255)

                                                    aaaGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACcac                                     >  1:3845680/1‑93 (MQ=255)

                                                       ggCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaaa                                  >  2:7161584/1‑93 (MQ=255)

                                                       ggCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACaaa                                  >  1:1956431/1‑93 (MQ=255)

                                                        gCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAAt                                 >  1:5332662/1‑93 (MQ=255)

                                                        gCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAAt                                 <  2:8565849/93‑1 (MQ=255)

                                                            aTAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgg                             >  2:6670359/1‑93 (MQ=255)

                                                            aTAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgg                             <  2:3608229/93‑1 (MQ=255)

                                                            aTAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgg                             <  2:8118801/93‑1 (MQ=255)

                                                             tAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAggg                            >  1:8052296/1‑93 (MQ=255)

                                                              aaGTAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAgggg                           <  2:1526240/93‑1 (MQ=255)

                                                               aGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACTACAAATCAgtgga                          >  2:4095534/1‑92 (MQ=255)

                                                               aGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGc                          >  1:8411357/1‑93 (MQ=255)

                                                               aGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGc                          >  2:2477000/1‑93 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                         <  1:5670575/93‑1 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                         <  1:6270970/93‑1 (MQ=255)

                                                                gCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCt                         >  1:4751175/1‑93 (MQ=255)

                                                                 cAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt                        <  1:593890/93‑1 (MQ=255)

                                                                 cAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCtt                        <  2:103922/93‑1 (MQ=255)

                                                                    aaaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCATTATCCAACCACAAATCAGGGGCTTGaa                     >  2:5664973/1‑93 (MQ=255)

                                                                    aaaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGaa                     <  1:727793/93‑1 (MQ=255)

                                                                     aaGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAAt                    <  1:4858551/93‑1 (MQ=255)

                                                                      aGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATc                   >  1:6818997/1‑93 (MQ=255)

                                                                       gCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATcc                  <  1:2807628/93‑1 (MQ=255)

                                                                          gTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta               >  1:4208310/1‑93 (MQ=255)

                                                                          gTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta               <  1:2125859/93‑1 (MQ=255)

                                                                          ctCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTta               <  1:6469669/92‑1 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa          <  1:3435026/93‑1 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa          <  1:4416111/93‑1 (MQ=255)

                                                                               cGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGa          <  1:6030411/93‑1 (MQ=255)

                                                                                gATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGaa         <  1:1471540/93‑1 (MQ=255)

                                                                                  tatTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgc       <  2:7319715/93‑1 (MQ=255)

                                                                                  tatTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgc       <  2:4252706/93‑1 (MQ=255)

                                                                                    ttGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAgcgc     >  1:3332064/1‑93 (MQ=255)

                                                                                     tgtgGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGcc    <  2:5080411/93‑1 (MQ=255)

                                                                                      gtggtgCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCg   <  2:27384/93‑1 (MQ=255)

TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGA  >  NC_000913/3458721‑3458900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  >  NC_000913/3458718‑3458904

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGT                                                                                               <  SRR4302541.4480810/94‑1 (MQ=60)

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGT                                                                                               <  SRR4302541.3769803/94‑1 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              <  SRR4302541.317712/94‑1 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              <  SRR4302541.7817206/94‑1 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              >  SRR4302541.3542653/1‑94 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              <  SRR4302541.3808522/94‑1 (MQ=60)

 CATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              >  SRR4302541.230685/1‑94 (MQ=60)

 CATTATCGGCATACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTG                                                                                              <  SRR4302541.364883/94‑1 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302541.1471543/1‑94 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302541.1471544/1‑94 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302541.8780254/1‑94 (MQ=60)

  ATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGC                                                                                             >  SRR4302541.8333280/1‑94 (MQ=60)

   TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCT                                                                                            <  SRR4302541.1947291/94‑1 (MQ=60)

   TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCT                                                                                            <  SRR4302541.577706/94‑1 (MQ=60)

   TTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCT                                                                                            <  SRR4302541.2282015/94‑1 (MQ=60)

      TCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGA                                                                                         <  SRR4302541.5564482/94‑1 (MQ=60)

       CGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAA                                                                                        >  SRR4302541.8609736/1‑94 (MQ=60)

       CGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAA                                                                                        >  SRR4302541.3545355/1‑94 (MQ=60)

       CGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAA                                                                                        <  SRR4302541.7728058/94‑1 (MQ=60)

       CGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAACCCGTCACCGATATTGGGGTGCTGGAA                                                                                        >  SRR4302541.3903528/1‑94 (MQ=60)

        GGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAA                                                                                       >  SRR4302541.2502684/1‑94 (MQ=60)

        GGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAA                                                                                       >  SRR4302541.6272043/1‑94 (MQ=60)

         GCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAA                                                                                      >  SRR4302541.5807198/1‑94 (MQ=60)

          CGTACTTGCCAGGCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     <  SRR4302541.5033794/94‑1 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     >  SRR4302541.3243715/1‑94 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     <  SRR4302541.6128851/94‑1 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     >  SRR4302541.917506/1‑94 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAAC                                                                                     >  SRR4302541.1470597/1‑94 (MQ=60)

          CGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTCTGGTGCTGGAAAAC                                                                                     >  SRR4302541.108412/1‑94 (MQ=60)

           GTACTTGCCAGTCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACG                                                                                    >  SRR4302541.1229978/1‑94 (MQ=60)

           GTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACG                                                                                    >  SRR4302541.3281075/1‑94 (MQ=60)

           GTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACG                                                                                    <  SRR4302541.5367651/94‑1 (MQ=60)

            TACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCTTCAGCGATATTGTGGTGCTGGAAAACGC                                                                                   >  SRR4302541.6277956/1‑94 (MQ=60)

            TACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGC                                                                                   <  SRR4302541.3339753/94‑1 (MQ=60)

            TACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGC                                                                                   <  SRR4302541.935569/94‑1 (MQ=60)

             TCTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCC                                                                                  <  SRR4302541.5350643/94‑1 (MQ=60)

             ACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCC                                                                                  >  SRR4302541.5943140/1‑94 (MQ=60)

              CTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCC                                                                                 >  SRR4302541.6184227/1‑94 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGTCGTACAAGCAGAAGGCGGCAAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302541.5409107/94‑1 (MQ=53)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302541.5420725/94‑1 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                <  SRR4302541.3142715/94‑1 (MQ=60)

               TTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCT                                                                                >  SRR4302541.6228216/1‑94 (MQ=60)

                TGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTT                                                                               <  SRR4302541.5470550/94‑1 (MQ=60)

                 GCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTG                                                                              <  SRR4302541.4714296/94‑1 (MQ=60)

                 GCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTG                                                                              >  SRR4302541.2116563/1‑94 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            <  SRR4302541.3562550/94‑1 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            <  SRR4302541.5736032/94‑1 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            >  SRR4302541.4601710/1‑94 (MQ=60)

                   CAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGAT                                                                            <  SRR4302541.6996614/94‑1 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           >  SRR4302541.1627189/1‑94 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           >  SRR4302541.1424121/1‑94 (MQ=60)

                    AGCCTGGTGGTTCCTAACCTAATGCGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATA                                                                           <  SRR4302541.665875/94‑1 (MQ=60)

                     GCCTGGTGTGTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATAT                                                                          <  SRR4302541.1824962/94‑1 (MQ=60)

                     GCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATAT                                                                          <  SRR4302541.3994615/94‑1 (MQ=60)

                      CCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATG                                                                         <  SRR4302541.926087/94‑1 (MQ=60)

                      CCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATG                                                                         <  SRR4302541.5915786/94‑1 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       <  SRR4302541.3634015/94‑1 (MQ=60)

                        TGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTA                                                                       >  SRR4302541.6508022/1‑94 (MQ=60)

                           TGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAA                                                                    <  SRR4302541.2354025/94‑1 (MQ=60)

                            GGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAA                                                                   <  SRR4302541.4231057/94‑1 (MQ=60)

                            GGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGGGCTGGAAAACGCCCTTGATATGTACAAA                                                                   >  SRR4302541.4159782/1‑94 (MQ=60)

                              TTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACT                                                                 >  SRR4302541.1518026/1‑94 (MQ=60)

                              TTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACT                                                                 >  SRR4302541.1518027/1‑94 (MQ=60)

                               tcctagcctaatgggcaataaagaaatttttttcaagcagaagacggcatacgagATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTC                                                                <  SRR4302541.486109/39‑1 (MQ=60)

                               TCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGCTATTGTGGTGCTGGAAACCGCCCTTGATATGTACACACTC                                                                >  SRR4302541.6361976/1‑94 (MQ=60)

                                CCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCG                                                               >  SRR4302541.7051048/1‑94 (MQ=60)

                                  TAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGAC                                                             <  SRR4302541.1632695/94‑1 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          <  SRR4302541.3939155/94‑1 (MQ=60)

                                     CCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAAT                                                          <  SRR4302541.1101104/94‑1 (MQ=60)

                                      CTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATC                                                         >  SRR4302541.4094143/1‑94 (MQ=60)

                                      CTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATC                                                         >  SRR4302541.3132980/1‑94 (MQ=60)

                                      CTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATC                                                         >  SRR4302541.7874336/1‑94 (MQ=60)

                                      CTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATC                                                         >  SRR4302541.4390751/1‑94 (MQ=60)

                                        AATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCAT                                                       <  SRR4302541.5779434/94‑1 (MQ=60)

                                         ATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATC                                                      <  SRR4302541.8649712/94‑1 (MQ=60)

                                          TGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCA                                                     <  SRR4302541.703242/94‑1 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAC                                                    >  SRR4302541.8241787/1‑94 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAC                                                    >  SRR4302541.6836242/1‑94 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCAC                                                    <  SRR4302541.1050557/94‑1 (MQ=60)

                                           GGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGGGCTGGAAAACGCCCTTGATATGTACAACATAGACCATCATCAC                                                    >  SRR4302541.6836243/1‑94 (MQ=60)

                                            GGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACT                                                   <  SRR4302541.8830861/94‑1 (MQ=60)

                                            GGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACT                                                   <  SRR4302541.2030997/94‑1 (MQ=60)

                                            GGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACT                                                   >  SRR4302541.365429/1‑94 (MQ=60)

                                            GGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACT                                                   >  SRR4302541.7856879/1‑94 (MQ=60)

                                            GGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACT                                                   >  SRR4302541.5392258/1‑94 (MQ=60)

                                              CAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTAT                                                 <  SRR4302541.5322821/94‑1 (MQ=60)

                                               AATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATC                                                >  SRR4302541.411739/1‑94 (MQ=60)

                                                 AAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCA                                              <  SRR4302541.3149146/94‑1 (MQ=60)

                                                    AGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATCTTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACC                                           >  SRR4302541.1834176/1‑94 (MQ=60)

                                                    AGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACC                                           >  SRR4302541.1746817/1‑94 (MQ=60)

                                                    AGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACC                                           <  SRR4302541.3675664/94‑1 (MQ=60)

                                                     GAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCA                                          <  SRR4302541.4884439/94‑1 (MQ=60)

                                                      AAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGTAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCAC                                         >  SRR4302541.5668042/1‑94 (MQ=60)

                                                      AAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCAC                                         >  SRR4302541.3884402/1‑94 (MQ=60)

                                                       AAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATTCAACCACA                                        >  SRR4302541.992139/1‑94 (MQ=60)

                                                       AAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACA                                        >  SRR4302541.2905352/1‑94 (MQ=60)

                                                        AAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAA                                       >  SRR4302541.3017533/1‑94 (MQ=60)

                                                        AAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAA                                       >  SRR4302541.5137081/1‑94 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      <  SRR4302541.545610/94‑1 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      <  SRR4302541.5628697/94‑1 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      <  SRR4302541.1417649/94‑1 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      >  SRR4302541.1975289/1‑94 (MQ=60)

                                                         AGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAA                                      >  SRR4302541.1717103/1‑94 (MQ=60)

                                                          GGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAAT                                     >  SRR4302541.5387518/1‑94 (MQ=60)

                                                             GGATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAG                                  <  SRR4302541.3163896/94‑1 (MQ=60)

                                                              GATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGG                                 <  SRR4302541.3427759/94‑1 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                <  SRR4302541.1959323/94‑1 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                >  SRR4302541.8140813/1‑94 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                >  SRR4302541.1494805/1‑94 (MQ=60)

                                                               ATAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGG                                <  SRR4302541.925758/94‑1 (MQ=60)

                                                                TAAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGG                               <  SRR4302541.4492473/94‑1 (MQ=60)

                                                                 AAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGC                              >  SRR4302541.8502319/1‑94 (MQ=60)

                                                                 AAGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGC                              >  SRR4302541.7063353/1‑94 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCTACAATCATCACTATCCAACCACAAATCAGGGGCT                             <  SRR4302541.3907746/94‑1 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCT                             <  SRR4302541.8603349/94‑1 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCT                             >  SRR4302541.4799718/1‑94 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCT                             >  SRR4302541.370315/1‑94 (MQ=60)

                                                                  AGCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCT                             <  SRR4302541.4273204/94‑1 (MQ=60)

                                                                   GCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTT                            <  SRR4302541.5728457/94‑1 (MQ=60)

                                                                   GCAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTT                            <  SRR4302541.6334998/94‑1 (MQ=60)

                                                                    CAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTG                           >  SRR4302541.1784774/1‑94 (MQ=60)

                                                                    CAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTG                           <  SRR4302541.599488/94‑1 (MQ=60)

                                                                    CAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTG                           <  SRR4302541.3718078/94‑1 (MQ=60)

                                                                    CAAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTG                           >  SRR4302541.8796050/1‑94 (MQ=60)

                                                                     AAAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGA                          >  SRR4302541.7860285/1‑94 (MQ=60)

                                                                      AAAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACACATCAGGGCCTTGAC                         >  SRR4302541.6621767/1‑94 (MQ=60)

                                                                       AAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAAT                        <  SRR4302541.734693/94‑1 (MQ=60)

                                                                       AAAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAAT                        >  SRR4302541.5977981/1‑94 (MQ=60)

                                                                        AAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATC                       >  SRR4302541.958385/1‑94 (MQ=60)

                                                                        AAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATC                       <  SRR4302541.4908096/94‑1 (MQ=60)

                                                                        AAGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATC                       >  SRR4302541.6891964/1‑94 (MQ=60)

                                                                         AGCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCC                      >  SRR4302541.2768459/1‑94 (MQ=60)

                                                                          GCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCT                     <  SRR4302541.2836019/94‑1 (MQ=60)

                                                                          GCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCT                     >  SRR4302541.3655271/1‑94 (MQ=60)

                                                                          GCCGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCT                     >  SRR4302541.6972932/1‑94 (MQ=60)

                                                                            CGTTAGCGATATTGGGGTGCTGGAAAACGACCTTGATATGTACAAACTCGACAATCAGAACTATCCAACCACAAATCAGGTGGCTGAATCCTTA                   >  SRR4302541.4250157/1‑94 (MQ=57)

                                                                            CGTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTA                   >  SRR4302541.4250156/1‑94 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302541.2146422/94‑1 (MQ=60)

                                                                             GTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302541.6057267/94‑1 (MQ=60)

                                                                             CTCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAG                  <  SRR4302541.6535606/94‑1 (MQ=60)

                                                                              TCAGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGT                 <  SRR4302541.6836484/94‑1 (MQ=60)

                                                                                AGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTAGAAACTCAACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAA               >  SRR4302541.7534780/1‑94 (MQ=60)

                                                                                AGCGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAG               <  SRR4302541.2822105/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302541.6092179/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302541.3469342/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302541.5858651/94‑1 (MQ=60)

                                                                                  CGATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAA             <  SRR4302541.4460396/94‑1 (MQ=60)

                                                                                   GATATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAG            <  SRR4302541.1485976/94‑1 (MQ=60)

                                                                                      ATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         >  SRR4302541.1542789/1‑94 (MQ=60)

                                                                                      ATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         >  SRR4302541.151827/1‑94 (MQ=60)

                                                                                      ATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         >  SRR4302541.3365436/1‑94 (MQ=60)

                                                                                      ATTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGC         >  SRR4302541.6138566/1‑94 (MQ=60)

                                                                                       TTGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACTACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCC        >  SRR4302541.6039490/1‑94 (MQ=60)

                                                                                        TGTGGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCG       <  SRR4302541.3426489/94‑1 (MQ=60)

                                                                                           GGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACA    >  SRR4302541.8756024/1‑94 (MQ=60)

                                                                                           GGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACA    >  SRR4302541.7963608/1‑94 (MQ=60)

                                                                                           GGTGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACA    >  SRR4302541.687056/1‑94 (MQ=60)

                                                                                             TGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  <  SRR4302541.1145248/94‑1 (MQ=60)

                                                                                             TGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  <  SRR4302541.1028722/94‑1 (MQ=60)

TCATTATCGGCGTACTTGCCAGCCTGGTGGTTCCTAACCTAATGGGCAATAAAGAAAAGGCGGATAAGCAAAAAGCCGTCAGCGATATTGTGGCGCTGGAAAACGCCCTTGATATGTACAAACTCGACAATCATCACTATCCAACCACAAATCAGGGGCTTGAATCCTTAGTAGAAGCGCCGACACT  >  NC_000913/3458718‑3458904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`