Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,4041.C99.7% 1197.5 / ‑4.1 351intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (192/158);  total (192/159)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01
*NC_0009133,537,4042.G99.7% 1203.9 / ‑4.1 352intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (192/159);  total (192/160)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 395 total aligned reads displayed.

GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487
                                                                                             ||                                                                                    
gcgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCa                                                                                        <  1:4762886/93‑1 (MQ=255)
 cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                                     >  1:5229148/1‑93 (MQ=255)
 cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                                     >  2:303769/1‑93 (MQ=255)
 cgcTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                                     <  1:433311/93‑1 (MQ=255)
   cTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgc                                                                                   <  1:5702058/93‑3 (MQ=255)
   cTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgc                                                                                   >  2:7578277/1‑91 (MQ=255)
     ggcggcggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgccg                                                                                 >  1:8812547/1‑89 (MQ=255)
           ggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTg                                                                             >  2:5643074/1‑93 (MQ=38)
           ggcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTg                                                                             >  1:6521924/1‑93 (MQ=38)
            gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTgg                                                                            >  1:3823393/1‑93 (MQ=38)
            gcAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTgg                                                                            <  1:3207686/93‑1 (MQ=38)
              aaTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCa                                                                          >  2:5967565/1‑93 (MQ=37)
               aTGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaa                                                                         >  1:197683/1‑93 (MQ=37)
                 gggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacg                                                                       >  2:4221753/1‑93 (MQ=37)
                   gCAAAAAACGTTAACCGCCCGTACTGTCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTCCACGCCGCATCTGGCaacgaa                                                                     <  1:4958079/93‑1 (MQ=37)
                   gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaa                                                                     <  1:4504676/93‑1 (MQ=38)
                    cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaac                                                                    >  1:2149998/1‑93 (MQ=37)
                    cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaac                                                                    <  2:3398820/93‑1 (MQ=37)
                     aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaacg                                                                   >  1:468724/1‑93 (MQ=37)
                     aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCaacgaacg                                                                   <  1:5766034/93‑1 (MQ=38)
                      aaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGc                                                                  <  1:7965059/93‑1 (MQ=37)
                       aaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGcc                                                                 <  2:195733/93‑1 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCt                                                                >  1:8377606/1‑93 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCt                                                                >  2:2368442/1‑93 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCt                                                                <  2:7769336/93‑1 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCt                                                                >  1:6241760/1‑93 (MQ=37)
                        aaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCt                                                                >  1:3463568/1‑93 (MQ=37)
                         aaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCtt                                                               >  1:390267/1‑93 (MQ=37)
                         aaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCtt                                                               <  2:8395821/93‑1 (MQ=37)
                         aaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCtt                                                               >  2:6223203/1‑93 (MQ=37)
                          aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTg                                                              >  2:5446033/1‑93 (MQ=37)
                          aCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTg                                                              >  2:4304058/1‑93 (MQ=37)
                            gTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGcc                                                            >  2:6862149/1‑93 (MQ=37)
                             ttAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCt                                                           >  1:124402/1‑93 (MQ=38)
                             taaaaCGCCGGTACTGGCCTCGGGTGCGATTTTGCACGAAATCGTAGGGAAGATAGGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCt                                                           <  2:3304091/91‑1 (MQ=21)
                              tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTg                                                          >  2:4899981/1‑93 (MQ=38)
                              tAAACGCCCGTACTGGCCTACGGTTCGAATTGGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTg                                                          <  2:2833041/93‑1 (MQ=37)
                               aaaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGa                                                         <  1:1643865/93‑1 (MQ=37)
                                 aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACa                                                       >  2:1124116/1‑93 (MQ=38)
                                  cGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACAt                                                      >  1:7186018/1‑93 (MQ=37)
                                     ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaa                                                   >  1:3980069/1‑93 (MQ=38)
                                      cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                  <  2:1584176/93‑1 (MQ=37)
                                      cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                  <  1:7766640/93‑1 (MQ=37)
                                      cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                                  <  1:8464323/93‑1 (MQ=37)
                                      cGTACTGCCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGACTGACATaaaa                                                  <  2:8687529/93‑1 (MQ=25)
                                       gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                                                 >  2:49737/1‑93 (MQ=38)
                                        tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                                                >  1:4869133/1‑93 (MQ=38)
                                          cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc                                              <  1:8730716/93‑1 (MQ=37)
                                          cTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGc                                              <  1:3709197/93‑1 (MQ=37)
                                           tGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                                             <  1:2788783/93‑1 (MQ=37)
                                            ggCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg                                            >  1:7138040/1‑93 (MQ=38)
                                            ggCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg                                            <  2:3882222/93‑1 (MQ=37)
                                               cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                         >  1:3076894/1‑93 (MQ=37)
                                               cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                         >  2:7056071/1‑93 (MQ=38)
                                               cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                                         >  1:1616200/1‑93 (MQ=38)
                                                tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgaga                                        >  1:290370/1‑93 (MQ=38)
                                                  cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAat                                      >  1:4599844/1‑93 (MQ=37)
                                                  cGGTTCGAATTTGCACGAAATCGAAGGGCAGATAAAGCGTTCACGCCGCATCTAGCAAGAAACTCCCTTCCTGACATAAAAGAGCCGGAGAat                                      >  1:1224703/1‑93 (MQ=19)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                     <  1:1094508/93‑1 (MQ=37)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                     <  2:145035/93‑1 (MQ=37)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                     <  2:5314278/93‑1 (MQ=37)
                                                   ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAata                                     >  2:4686569/1‑93 (MQ=38)
                                                     ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                                   >  1:4378274/1‑93 (MQ=38)
                                                      tCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCGTTGCCTGACATAAAAGTGCCGGAGAATAtc‑t                                 <  2:7395367/93‑1 (MQ=37)
                                                      gcGAATTGGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc‑t                                 <  2:3572517/92‑1 (MQ=37)
                                                       cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc‑tc                                <  1:2470415/93‑1 (MQ=37)
                                                        gAATTTGCAGGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑Tcc                               <  2:3163876/93‑1 (MQ=37)
                                                         aagttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCg                              <  1:7313237/90‑1 (MQ=37)
                                                         aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCg                              <  1:4061703/93‑1 (MQ=37)
                                                           tttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGc                            >  1:7456967/1‑93 (MQ=38)
                                                            ttGCACGAAATCGTAGGGCAGATAAGGGGTTCACGCCGCATCTGGAAACGAAGGCTTTGCCTGAAATAAAAG‑GGCGGGGGATATCTTCCGGct                           >  1:4400612/1‑92 (MQ=12)
                                                            ttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCa                           <  1:8492297/93‑1 (MQ=38)
                                                             tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCAt                          >  2:7993136/1‑93 (MQ=38)
                                                               cACGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACGTAAAAGTGCCGGAGAATATC‑TCCGGCAttt                        <  1:2328280/93‑1 (MQ=37)
                                                                 cGAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCAttttt                      >  1:6725113/1‑93 (MQ=38)
                                                                  gAAATCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTa                     >  1:5182497/1‑93 (MQ=38)
                                                                   aaaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTAt                    >  2:4614909/1‑93 (MQ=38)
                                                                   aaaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTAt                    <  2:7166223/93‑1 (MQ=38)
                                                                   aaaTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTAt                    <  2:2572739/93‑1 (MQ=38)
                                                                     aTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTc                  <  2:4035463/93‑1 (MQ=38)
                                                                     aTCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTc                  <  1:4153381/93‑1 (MQ=38)
                                                                      tCGTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTcc                 <  1:6867851/93‑1 (MQ=38)
                                                                        gTAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCcac               <  1:8066422/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCcaca              <  1:5616589/93‑1 (MQ=38)
                                                                         tAGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCcaca              >  1:3525835/1‑93 (MQ=38)
                                                                         gaGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCcaca              <  2:3698497/92‑1 (MQ=38)
                                                                          aGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAg             <  1:533747/93‑1 (MQ=38)
                                                                          aGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAg             >  1:1451927/1‑93 (MQ=38)
                                                                          aGGGCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAg             <  1:3657944/93‑1 (MQ=38)
                                                                           gggCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGc            >  2:5217750/1‑93 (MQ=38)
                                                                             gCAGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCa          >  1:5729319/1‑93 (MQ=38)
                                                                               aGATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCaaa        <  2:8132798/93‑1 (MQ=38)
                                                                               aGATAAGGCGTCCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCaaa        <  2:1904859/93‑1 (MQ=38)
                                                                                gATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAAc       <  1:5935813/93‑1 (MQ=38)
                                                                                gATAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAAc       >  1:7640345/1‑93 (MQ=39)
                                                                                 aTAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACt      <  2:6456342/93‑1 (MQ=38)
                                                                                 aTAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACt      >  1:5445379/1‑93 (MQ=39)
                                                                                 aTAAGGCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACt      <  2:1280566/93‑1 (MQ=38)
                                                                                     ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACTCata  >  1:2661455/1‑93 (MQ=255)
                                                                                     ggCGTTCACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACTCata  <  1:1761092/93‑1 (MQ=39)
                                                                                             ||                                                                                    
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC‑TCCGGCATTTTTATTCCACAGCCAAACTCATA  >  NC_000913/3537312‑3537487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 275 total aligned reads displayed.
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498
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GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               <  SRR4302541.5324143/94‑1 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               <  SRR4302541.7233105/94‑1 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               <  SRR4302541.6872081/94‑1 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               >  SRR4302541.5283028/1‑94 (MQ=60)
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               <  SRR4302541.4811530/94‑1 (MQ=60)
GCGCTGGCGGCAGCAATGGGCAAAAAACGTTAAACGCTCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑C                                                                                               <  SRR4302541.8759791/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑CG                                                                                              >  SRR4302541.3253742/1‑94 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑CG                                                                                              <  SRR4302541.437388/94‑1 (MQ=60)
 CGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑CG                                                                                              <  SRR4302541.1671341/94‑1 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgc                                                                                             >  SRR4302541.540387/1‑92 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgc                                                                                             >  SRR4302541.6182543/1‑92 (MQ=60)
  GCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgc                                                                                             >  SRR4302541.2538837/1‑92 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgcc                                                                                            <  SRR4302541.5760207/94‑4 (MQ=60)
   CTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgcc                                                                                            <  SRR4302541.4415674/94‑4 (MQ=60)
    TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccg                                                                                           >  SRR4302541.3904317/1‑90 (MQ=60)
    TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccg                                                                                           <  SRR4302541.6421606/94‑5 (MQ=60)
    TGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccg                                                                                           <  SRR4302541.8578623/94‑5 (MQ=60)
     GGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccgc                                                                                          >  SRR4302541.4718471/1‑89 (MQ=60)
     GGCGGCGGCAATGGGAAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccgc                                                                                          <  SRR4302541.4830560/94‑6 (MQ=60)
        GGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATC                                                                                         <  SRR4302541.3792369/94‑1 (MQ=60)
        GGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATC                                                                                         >  SRR4302541.6803524/1‑94 (MQ=60)
         GCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCT                                                                                        <  SRR4302541.971802/94‑1 (MQ=60)
         GCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCT                                                                                        <  SRR4302541.8455780/94‑1 (MQ=60)
          GGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTG                                                                                       <  SRR4302541.1417937/94‑1 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTG                                                                                       >  SRR4302541.3944521/1‑94 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTG                                                                                       >  SRR4302541.5270695/1‑94 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTG                                                                                       >  SRR4302541.71745/1‑94 (MQ=60)
          CGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTG                                                                                       >  SRR4302541.6588473/1‑94 (MQ=60)
             CAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCA                                                                                    <  SRR4302541.2642529/94‑1 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAA                                                                                   >  SRR4302541.199179/1‑94 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAA                                                                                   >  SRR4302541.4750137/1‑94 (MQ=60)
              AATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGCAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑Cgccgcatccggcac                                                                                 >  SRR4302541.2479752/1‑80 (MQ=60)
               ATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAAC                                                                                  >  SRR4302541.7755003/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACG                                                                                 >  SRR4302541.5546666/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACG                                                                                 >  SRR4302541.8028944/1‑94 (MQ=60)
                TGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACG                                                                                 <  SRR4302541.840379/94‑1 (MQ=60)
                 GGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGA                                                                                >  SRR4302541.5779820/1‑94 (MQ=60)
                  GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAA                                                                               <  SRR4302541.413134/94‑1 (MQ=60)
                   aaaaaaaccttaAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAAC                                                                              <  SRR4302541.5008815/82‑1 (MQ=60)
                   GCAAAAAACGTTAACCGCCCGTACTGTCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TC‑‑CACGCCGCATCTGGCAACGAAC                                                                              <  SRR4302541.5008814/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAAC                                                                              <  SRR4302541.4550119/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAAC                                                                              <  SRR4302541.4047597/94‑1 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAAC                                                                              >  SRR4302541.1892150/1‑94 (MQ=60)
                   GCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAAC                                                                              >  SRR4302541.2170789/1‑94 (MQ=60)
                    CAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACG                                                                             <  SRR4302541.3555413/94‑1 (MQ=60)
                     AAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGC                                                                            >  SRR4302541.3164999/1‑94 (MQ=60)
                     AAAAAACGTTAAACGCCCGGACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGC                                                                            <  SRR4302541.1246518/94‑1 (MQ=60)
                      AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCC                                                                           >  SRR4302541.1697669/1‑94 (MQ=60)
                      AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCC                                                                           <  SRR4302541.8052840/94‑1 (MQ=60)
                      AAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCC                                                                           >  SRR4302541.5387263/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCT                                                                          >  SRR4302541.8660352/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCT                                                                          >  SRR4302541.2393597/1‑94 (MQ=60)
                       AAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCT                                                                          >  SRR4302541.6305529/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATACGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTT                                                                         >  SRR4302541.8563644/1‑94 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTT                                                                         <  SRR4302541.4607755/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTT                                                                         <  SRR4302541.4607756/94‑1 (MQ=60)
                        AAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTT                                                                         <  SRR4302541.7412994/94‑1 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCC                                                                      >  SRR4302541.8220678/1‑94 (MQ=60)
                           CGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCC                                                                      >  SRR4302541.5732977/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                     >  SRR4302541.125385/1‑94 (MQ=60)
                            GTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCT                                                                     <  SRR4302541.4678211/94‑1 (MQ=60)
                              TAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGA                                                                   <  SRR4302541.885019/94‑1 (MQ=60)
                              TAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGA                                                                   >  SRR4302541.5236227/1‑94 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  <  SRR4302541.3631391/94‑1 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  <  SRR4302541.1660117/94‑1 (MQ=60)
                               AAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGAC                                                                  <  SRR4302541.2895860/94‑1 (MQ=60)
                                AACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACA                                                                 >  SRR4302541.7667438/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGCACGCCTTGCCTGACAT                                                                >  SRR4302541.6515361/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                >  SRR4302541.7262678/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                >  SRR4302541.4425416/1‑94 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                <  SRR4302541.3871001/94‑1 (MQ=60)
                                 ACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACAT                                                                >  SRR4302541.5468337/1‑94 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               >  SRR4302541.5843373/1‑94 (MQ=60)
                                  CGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATA                                                               >  SRR4302541.4256831/1‑94 (MQ=60)
                                    CCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAA                                                             >  SRR4302541.4019531/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                           >  SRR4302541.2802200/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                           >  SRR4302541.7184821/1‑94 (MQ=60)
                                      CGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAG                                                           <  SRR4302541.7849263/94‑1 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302541.4918808/1‑94 (MQ=60)
                                       GTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGT                                                          >  SRR4302541.4843705/1‑94 (MQ=60)
                                        TACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTG                                                         <  SRR4302541.3050145/94‑1 (MQ=60)
                                          CTTGCCTACGGTTTGAATTTGCCCGAAATCGTAGGGCAGATAAGGCG‑‑TTCCCG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                       <  SRR4302541.7217050/94‑1 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                       >  SRR4302541.5699055/1‑94 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                       <  SRR4302541.8824193/94‑1 (MQ=60)
                                          CTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCC                                                       <  SRR4302541.3746634/94‑1 (MQ=60)
                                           TGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCG                                                      <  SRR4302541.2816928/94‑1 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302541.2191280/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302541.3108113/1‑94 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   <  SRR4302541.2446442/94‑1 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   <  SRR4302541.6962431/94‑1 (MQ=60)
                                              CCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAG                                                   >  SRR4302541.4271186/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302541.4654922/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302541.292881/1‑94 (MQ=60)
                                               CTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGA                                                  >  SRR4302541.2896120/1‑94 (MQ=60)
                                                TACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAA                                                 <  SRR4302541.2110827/94‑1 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                >  SRR4302541.4646525/1‑94 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                >  SRR4302541.5778242/1‑94 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                >  SRR4302541.1236712/1‑94 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAAT                                                <  SRR4302541.1518992/94‑1 (MQ=60)
                                                 ACGGTTCGAATTTGCACGAAATCGAAGGGCAGATAAAGCG‑‑TT‑‑CACGCCGCATCTAGCAAGAAACTCCCTTCCTGACATAAAAGAGCCGGAGAAT                                                >  SRR4302541.1236710/1‑94 (MQ=26)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCGGATAAGGCG‑‑TT‑‑CACGCCGAATATGGCAAAGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               <  SRR4302541.7868200/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               <  SRR4302541.7354721/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               <  SRR4302541.5544761/94‑1 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302541.3383714/1‑94 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302541.5962616/1‑94 (MQ=60)
                                                  CGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATA                                               >  SRR4302541.1471287/1‑94 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              <  SRR4302541.1104738/94‑1 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              >  SRR4302541.8913347/1‑94 (MQ=60)
                                                   GGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              >  SRR4302541.2269486/1‑94 (MQ=60)
                                                   GGTTCGAATTTACACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAT                                              <  SRR4302541.5888566/94‑1 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTTACATAAAAGTGCCGGAGAATATC                                             >  SRR4302541.4414621/1‑94 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302541.1886000/1‑94 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302541.385677/1‑94 (MQ=60)
                                                    GTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATC                                             >  SRR4302541.4422025/1‑94 (MQ=60)
                                                     TTCTAATTTGCACGAAATCGTAGGGCAAATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCT                                            <  SRR4302541.130061/94‑1 (MQ=60)
                                                      TCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTC                                           >  SRR4302541.3714402/1‑94 (MQ=60)
                                                       CGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCG                                          >  SRR4302541.3009753/1‑94 (MQ=60)
                                                       CGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCC                                          <  SRR4302541.2495129/94‑1 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         >  SRR4302541.3498266/1‑94 (MQ=60)
                                                        GAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCG                                         <  SRR4302541.1647123/94‑1 (MQ=60)
                                                         AATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        <  SRR4302541.4101787/94‑1 (MQ=60)
                                                         AATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        <  SRR4302541.7391307/94‑1 (MQ=60)
                                                         AATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCCGAGAATAattcccg                                        >  SRR4302541.6315915/1‑87 (MQ=60)
                                                         AAGTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGG                                        <  SRR4302541.7391308/94‑1 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302541.7536607/1‑94 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGC                                       >  SRR4302541.8528350/1‑94 (MQ=60)
                                                          ATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTACGGC                                       >  SRR4302541.6656754/1‑94 (MQ=60)
                                                           TTTGCACGAAATCGTAGGGCAGATAAGGGG‑‑TT‑‑CACGCCGCATCTGGAAACGAAGGCTTTGCCTGAAATAAAAGggcgggggatatcttccggct                                      >  SRR4302541.4444627/1‑73 (MQ=37)
                                                           TTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCA                                      <  SRR4302541.8509765/94‑1 (MQ=60)
                                                           TTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTACGGCA                                      >  SRR4302541.4444629/1‑94 (MQ=60)
                                                             TGCACGAAATCGTAGTGGAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTACCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                    <  SRR4302541.1502529/94‑1 (MQ=60)
                                                             TGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATT                                    <  SRR4302541.3736020/94‑1 (MQ=60)
                                                              GCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTT                                   >  SRR4302541.1648459/1‑94 (MQ=60)
                                                               CACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACGTAAAAGTGCCGGAGAATATCTCCGGCATTTT                                  <  SRR4302541.2350875/94‑1 (MQ=60)
                                                               CACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                  >  SRR4302541.1411328/1‑94 (MQ=60)
                                                               CACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTT                                  >  SRR4302541.6764476/1‑94 (MQ=60)
                                                                ACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTT                                 >  SRR4302541.6796879/1‑94 (MQ=60)
                                                                 CGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                                >  SRR4302541.4430211/1‑94 (MQ=60)
                                                                 CGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                                <  SRR4302541.5212105/94‑1 (MQ=60)
                                                                 CGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTA                                >  SRR4302541.5235810/1‑94 (MQ=60)
                                                                   AAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                              <  SRR4302541.3602719/94‑1 (MQ=60)
                                                                   AAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT                              >  SRR4302541.8002893/1‑94 (MQ=60)
                                                                    AATCGTAGGTCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTC                             <  SRR4302541.4070546/94‑1 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302541.3973549/94‑1 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302541.8893658/94‑1 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302541.4194463/94‑1 (MQ=60)
                                                                     ATCGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCC                            <  SRR4302541.4111819/94‑1 (MQ=60)
                                                                       CGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                          >  SRR4302541.7811719/1‑94 (MQ=60)
                                                                       CGTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCAC                          >  SRR4302541.4903272/1‑94 (MQ=60)
                                                                        GTAGGGCAGATAGGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302541.8153690/94‑1 (MQ=60)
                                                                        GTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         <  SRR4302541.8155009/94‑1 (MQ=60)
                                                                        GTAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACA                         >  SRR4302541.7517030/1‑94 (MQ=60)
                                                                         TAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        <  SRR4302541.5674073/94‑1 (MQ=60)
                                                                         GAGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGTCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAG                        <  SRR4302541.5487764/94‑1 (MQ=60)
                                                                          AGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       <  SRR4302541.3695059/94‑1 (MQ=60)
                                                                          AGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       <  SRR4302541.538751/94‑1 (MQ=60)
                                                                          AGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       >  SRR4302541.3215700/1‑94 (MQ=60)
                                                                          AGGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGC                       >  SRR4302541.3754063/1‑94 (MQ=60)
                                                                           GGGCAGATAAGGCGTCTA‑‑CG‑‑CCGCATCTGGCACCGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCC                      <  SRR4302541.850452/94‑1 (MQ=60)
                                                                           GGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGGGCATATTTCCGGCATTTTTATTCTACACCC                      >  SRR4302541.4811432/1‑94 (MQ=60)
                                                                           GGGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCC                      <  SRR4302541.687951/94‑1 (MQ=60)
                                                                            GGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                     <  SRR4302541.8558178/94‑1 (MQ=60)
                                                                            GGCAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCA                     >  SRR4302541.5787725/1‑94 (MQ=60)
                                                                              CAGATAAGGGT‑‑TT‑‑CACCCCGCATCAGCCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                   <  SRR4302541.110361/94‑1 (MQ=60)
                                                                              CAGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAA                   >  SRR4302541.2720649/1‑94 (MQ=60)
                                                                               AGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302541.5795889/1‑94 (MQ=60)
                                                                               AGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302541.8077687/1‑94 (MQ=60)
                                                                               AGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302541.8896523/1‑94 (MQ=60)
                                                                               AGATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAAC                  >  SRR4302541.7721806/1‑94 (MQ=60)
                                                                                GATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACT                 <  SRR4302541.5996286/94‑1 (MQ=60)
                                                                                 ATAAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                >  SRR4302541.4940962/1‑94 (MQ=60)
                                                                                 ATAAGGCG‑‑TC‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTC                <  SRR4302541.3550609/94‑1 (MQ=60)
                                                                                   AAGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCAT              >  SRR4302541.3272762/1‑94 (MQ=60)
                                                                                    AGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302541.5812832/1‑94 (MQ=60)
                                                                                    AGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302541.2059495/1‑94 (MQ=60)
                                                                                    AGGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATA             >  SRR4302541.2687920/1‑94 (MQ=60)
                                                                                     GGCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAA            <  SRR4302541.1778242/94‑1 (MQ=60)
                                                                                      GCG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAAT           <  SRR4302541.7757577/94‑1 (MQ=60)
                                                                                       CG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA          >  SRR4302541.7020065/1‑94 (MQ=60)
                                                                                       CG‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATA          >  SRR4302541.8875553/1‑94 (MQ=60)
                                                                                        G‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTTCACAGCCAAACTCATAATAT         >  SRR4302541.7920956/1‑94 (MQ=60)
                                                                                        G‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         <  SRR4302541.7232796/94‑1 (MQ=60)
                                                                                        G‑‑TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATAT         >  SRR4302541.7380355/1‑94 (MQ=60)
                                                                                           TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA        >  SRR4302541.7667207/1‑94 (MQ=60)
                                                                                           TT‑‑CACGCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATA        >  SRR4302541.4731647/1‑94 (MQ=60)
                                                                                              tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATACTATAT       <  SRR4302541.8816212/89‑1 (MQ=60)
                                                                                              tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       <  SRR4302541.5118239/89‑1 (MQ=60)
                                                                                              tcacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATAT       >  SRR4302541.7184375/6‑94 (MQ=60)
                                                                                               cacgCCGCATCTGGCAACGAACTCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      <  SRR4302541.6404118/90‑1 (MQ=60)
                                                                                               cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      >  SRR4302541.1136691/5‑94 (MQ=60)
                                                                                               cacgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATT      <  SRR4302541.3930046/90‑1 (MQ=60)
                                                                                               CG‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCC    <  SRR4302541.4464992/94‑1 (MQ=60)
                                                                                                acgCCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTC     <  SRR4302541.5727485/91‑1 (MQ=60)
                                                                                                G‑‑CCGCATCTGGCAACGAACGCCTTGCCTTACATAAAAGTGCCGCAGGATATCTCCGGCCTTTTTATTCCACACGCAAAATCATAATATATTACG   >  SRR4302541.3595102/1‑94 (MQ=60)
                                                                                                G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGGATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATTTATTCCA   >  SRR4302541.6485285/1‑94 (MQ=60)
                                                                                                G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   >  SRR4302541.2117661/1‑94 (MQ=60)
                                                                                                G‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCG   <  SRR4302541.4327109/94‑1 (MQ=60)
                                                                                                   CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  SRR4302541.1356114/1‑94 (MQ=60)
                                                                                                   CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  <  SRR4302541.5746960/94‑1 (MQ=60)
                                                                                                   CCGCATCTGGCAACGAACGCCTTCCCTGACATAAAAGTGCCGGAGAAGATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  <  SRR4302541.6829837/94‑1 (MQ=60)
                                                                                                   |                                                                                             
GCGCTGGCGGCGGCAATGGGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCG‑‑TT‑‑CA‑‑CCGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATTCCACAGCCAAACTCATAATATATTCCGG  >  NC_000913/3537312‑3537498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑