Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9180GC99.9% 2268.6 / NA 653W116C (TGG→TGC) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (294/358);  total (295/358)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,980,9190CG99.9% 2268.4 / ‑6.4 661P117A (CCG→GCG) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (295/365);  minor base A (0/1);  total (295/366)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 679 total aligned reads displayed.

CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005
                                                                                       ||                                                                                      
cgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGtt                                                                                    >  1:2497769/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   <  1:3167340/93‑1 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  2:4640723/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  1:7824408/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  1:75445/1‑93 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  1:3805141/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:323227/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                <  2:1066475/93‑1 (MQ=255)
     cgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcg                                                                               >  2:8169384/1‑93 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             >  1:1223127/1‑93 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             >  2:7076076/1‑93 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             <  2:3719271/93‑1 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATAACGAATGGTGCGCGTTTATcgcg                                                                             >  2:4737052/1‑93 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  2:4008713/93‑1 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  1:1750838/93‑1 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  1:3662713/93‑1 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         <  2:6317469/93‑1 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         <  2:7545451/93‑1 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        <  2:5912880/93‑1 (MQ=255)
             gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       <  1:6095796/93‑1 (MQ=255)
             gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       >  2:6162694/1‑93 (MQ=255)
               gAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatca                                                                     <  2:4530758/93‑1 (MQ=255)
                  atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAttt                                                                  >  1:8395394/1‑93 (MQ=255)
                  atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAttt                                                                  <  1:6976338/93‑1 (MQ=255)
                   tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                 <  1:8517694/93‑1 (MQ=255)
                    atGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc                                                                <  1:3529315/93‑1 (MQ=255)
                    atGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTc                                                                >  2:2318721/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  2:7346715/93‑1 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               >  1:156718/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  1:661707/93‑1 (MQ=255)
                      ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                              >  2:8613720/1‑93 (MQ=255)
                      ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                              <  1:4096366/93‑1 (MQ=255)
                       gTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                             <  2:6691163/93‑1 (MQ=255)
                       gTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                             <  1:8623796/93‑1 (MQ=255)
                        tGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgt                                                            <  1:4483628/93‑1 (MQ=255)
                         gCTGGCAGGAAGATAATGAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGATCAACATTTTTctgtt                                                           >  2:7837601/1‑93 (MQ=255)
                         gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt                                                           <  1:6420209/93‑1 (MQ=255)
                         gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt                                                           >  2:5750684/1‑93 (MQ=255)
                          cTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgttg                                                          <  1:1022523/93‑1 (MQ=255)
                           tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTgg                                                         <  1:4229760/93‑1 (MQ=255)
                           tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTgg                                                         >  2:6043847/1‑93 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  1:3341535/93‑1 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  2:8341791/93‑1 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGCTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  1:7800269/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  1:4741752/93‑1 (MQ=255)
                              cAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGttt                                                      >  1:561484/1‑93 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     <  1:8019705/93‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     >  2:2099163/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGGGCATCATTTTATTGTTGGTTTAt                                                    >  2:2727424/1‑93 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  1:2151457/93‑1 (MQ=255)
                                ggAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAt                                                    <  2:2882534/93‑1 (MQ=255)
                                 gAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc                                                   <  2:4200171/93‑1 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  <  2:6942538/93‑1 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCgt                                                 >  1:2998181/1‑92 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                 <  2:4916451/93‑1 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  1:2645712/66‑1 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                <  2:5270945/93‑1 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                >  2:8740631/1‑93 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                >  2:8433282/1‑93 (MQ=255)
                                     aTAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTg                                               <  2:5577869/93‑1 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  1:943095/1‑93 (MQ=255)
                                      tAAGGAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              <  2:1843852/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           <  2:7960676/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           >  1:5017052/1‑93 (MQ=255)
                                          tAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATgg                                          >  2:7262596/1‑93 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGc                                         <  2:4298590/93‑1 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    >  1:1930753/1‑93 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  1:734167/93‑1 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  1:8139948/93‑1 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  2:6492330/93‑1 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   >  1:2257686/1‑93 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  2:1657310/93‑1 (MQ=255)
                                                  tCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTg                                  >  1:683356/1‑93 (MQ=255)
                                                    cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag                                <  2:5132566/93‑1 (MQ=255)
                                                     aGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgaga                               <  1:5802982/93‑1 (MQ=255)
                                                      gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAt                              <  1:6041020/93‑1 (MQ=255)
                                                      gACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGCTTATCGCTGGATGGCCCGCCTGAGAt                              >  1:5188416/1‑93 (MQ=255)
                                                         tAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCca                           >  1:5351930/1‑93 (MQ=255)
                                                          aaCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                          <  2:2575595/93‑1 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       >  1:2824326/1‑93 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      <  2:7627396/93‑1 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGAGCCAcaatc                      >  2:3363065/1‑93 (MQ=255)
                                                                  gctgctGTATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  <  1:1986950/93‑1 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  >  1:7142210/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  <  1:4943856/93‑1 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  <  2:3580661/93‑1 (MQ=255)
                                                                   ctgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAatat                 <  2:5445833/93‑1 (MQ=255)
                                                                       tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgcg             >  1:138569/1‑93 (MQ=255)
                                                                        ggATGACGAATGTTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:6248980/93‑1 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:8798625/93‑1 (MQ=255)
                                                                         gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTg           <  2:2223755/93‑1 (MQ=255)
                                                                           tGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAc         <  2:8539896/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  1:7463208/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       >  2:1440230/1‑93 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  2:3143234/93‑1 (MQ=255)
                                                                              cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaa      <  2:1116195/93‑1 (MQ=255)
                                                                              cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaa      <  1:1804461/93‑1 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAATAAAg    >  2:1238202/1‑93 (MQ=255)
                                                                                  tggtgcGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt  <  1:2335075/93‑1 (MQ=255)
                                                                                  tggtgcGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGt  <  1:5941024/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 352 total aligned reads displayed.
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007
                                                                                             |                                                                                         
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGG‑GGC                                                                                          >  SRR4302541.680310/1‑94 (MQ=60)
 CGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCC                                                                                          >  SRR4302541.7264543/1‑94 (MQ=60)
      CGTAGCGTTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302541.3487265/94‑1 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     >  SRR4302541.2350847/1‑94 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     >  SRR4302541.76087/1‑94 (MQ=60)
       GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTC                                                                                    >  SRR4302541.7901641/1‑94 (MQ=60)
       GTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTC                                                                                    <  SRR4302541.5168776/94‑1 (MQ=60)
        TAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCT                                                                                   <  SRR4302541.8149993/94‑1 (MQ=60)
        TAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCT                                                                                   >  SRR4302541.664062/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302541.957638/94‑1 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302541.3843448/94‑1 (MQ=60)
          GCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCG                                                                                 >  SRR4302541.6172204/1‑94 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                <  SRR4302541.253743/94‑1 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                <  SRR4302541.704087/94‑1 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                <  SRR4302541.8842771/94‑1 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                >  SRR4302541.5033064/1‑94 (MQ=60)
            GTTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCG                                                                               <  SRR4302541.6909483/94‑1 (MQ=60)
            GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCG                                                                               <  SRR4302541.7886673/94‑1 (MQ=60)
             CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              >  SRR4302541.5889932/1‑94 (MQ=60)
             CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              >  SRR4302541.8709002/1‑94 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             <  SRR4302541.3772927/94‑1 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             <  SRR4302541.105291/94‑1 (MQ=60)
              TACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGA                                                                             <  SRR4302541.1787223/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302541.3713314/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302541.2396593/94‑1 (MQ=60)
                CAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGC                                                                           <  SRR4302541.6119996/94‑1 (MQ=60)
                  GGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCAT                                                                         <  SRR4302541.186986/94‑1 (MQ=60)
                  GGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCAT                                                                         <  SRR4302541.6459007/94‑1 (MQ=60)
                  GGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCAT                                                                         >  SRR4302541.3811411/1‑94 (MQ=60)
                   GCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATC                                                                        <  SRR4302541.3214786/94‑1 (MQ=60)
                   GCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATA                                                                        >  SRR4302541.2556197/1‑94 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       >  SRR4302541.924463/1‑94 (MQ=60)
                      AAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGAGTGCTGCTGGATGACGAATGGTGGGCGTTTCTCGCGGCGCATCATT                                                                     >  SRR4302541.3098818/1‑94 (MQ=60)
                       AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    >  SRR4302541.8486259/1‑94 (MQ=60)
                       AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    >  SRR4302541.5696777/1‑94 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   >  SRR4302541.3925354/1‑94 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   <  SRR4302541.3409519/94‑1 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   >  SRR4302541.2102824/1‑94 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302541.4049160/94‑1 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302541.8609370/94‑1 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  >  SRR4302541.1973166/1‑94 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302541.3689575/94‑1 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 <  SRR4302541.1676294/94‑1 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 <  SRR4302541.8803099/94‑1 (MQ=60)
                           TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                <  SRR4302541.667951/94‑1 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               <  SRR4302541.4136801/94‑1 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               >  SRR4302541.2598758/1‑94 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               <  SRR4302541.5005142/94‑1 (MQ=60)
                             GTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              <  SRR4302541.2307581/94‑1 (MQ=60)
                             GTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              <  SRR4302541.2307582/94‑1 (MQ=60)
                             GGGCTGGCAGGAAGATAAGTAACAGCTTCCAGTCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              <  SRR4302541.6005935/94‑1 (MQ=60)
                              TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302541.1965646/94‑1 (MQ=60)
                              TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302541.4528819/94‑1 (MQ=60)
                               GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            <  SRR4302541.6485662/94‑1 (MQ=60)
                               GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            >  SRR4302541.8724888/1‑94 (MQ=60)
                               GCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTG                                                            <  SRR4302541.8487668/94‑1 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           >  SRR4302541.5892490/1‑94 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           <  SRR4302541.1716987/94‑1 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           <  SRR4302541.1031970/94‑1 (MQ=60)
                                 TGGCAGTAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGT                                                          <  SRR4302541.1789582/94‑1 (MQ=60)
                                 TGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGT                                                          <  SRR4302541.4271897/94‑1 (MQ=60)
                                 TGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGT                                                          <  SRR4302541.6993658/94‑1 (MQ=60)
                                 TGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGT                                                          <  SRR4302541.7823505/94‑1 (MQ=60)
                                  GGCAGGAAGATACGTAACAGCTCCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302541.902273/94‑1 (MQ=60)
                                  GGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302541.7504158/94‑1 (MQ=60)
                                  GGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGCTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302541.7884212/94‑1 (MQ=60)
                                   GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        <  SRR4302541.4790164/94‑1 (MQ=60)
                                   GCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTT                                                        >  SRR4302541.566763/1‑94 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       >  SRR4302541.5204270/1‑94 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       >  SRR4302541.3995884/1‑94 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       <  SRR4302541.3653237/94‑1 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       >  SRR4302541.8203380/1‑94 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       >  SRR4302541.7667170/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302541.368377/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302541.6417580/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302541.2916035/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302541.8107880/94‑1 (MQ=60)
                                      GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302541.2185876/94‑1 (MQ=60)
                                      GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302541.2172261/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302541.7132572/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302541.5260576/94‑1 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGT                                                   >  SRR4302541.3028442/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302541.623861/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302541.8405506/1‑94 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302541.4262656/1‑94 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302541.8540299/94‑1 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302541.6200791/1‑94 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302541.2703680/94‑1 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  <  SRR4302541.4678217/94‑1 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302541.1422797/1‑94 (MQ=60)
                                          GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 <  SRR4302541.4482350/94‑1 (MQ=60)
                                          GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 <  SRR4302541.8443845/94‑1 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                <  SRR4302541.4636893/94‑1 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                <  SRR4302541.2796032/94‑1 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                >  SRR4302541.5408076/1‑94 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                >  SRR4302541.7519498/1‑94 (MQ=60)
                                            TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGT                                               >  SRR4302541.1223634/1‑94 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATTGCTGGAG                                              >  SRR4302541.2137793/1‑94 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302541.715315/94‑1 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              <  SRR4302541.7217311/94‑1 (MQ=60)
                                             AAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAGTTTCTTGTTGGTTTATCGCTGGAT                                              >  SRR4302541.8038623/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             <  SRR4302541.23600/94‑1 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302541.5808370/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302541.5068624/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             <  SRR4302541.43106/94‑1 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302541.3189058/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             >  SRR4302541.7601097/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          <  SRR4302541.2436737/94‑1 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302541.7323354/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302541.7899534/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302541.3224071/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302541.1296051/1‑94 (MQ=60)
                                                  ACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCC                                         <  SRR4302541.4373772/94‑1 (MQ=60)
                                                   CAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCTCTGGATGGCCCG                                        >  SRR4302541.6890655/1‑94 (MQ=60)
                                                   AAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCG                                        <  SRR4302541.894669/94‑1 (MQ=60)
                                                    AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       >  SRR4302541.5605506/1‑94 (MQ=60)
                                                    AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCAC                                       <  SRR4302541.4992609/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302541.8632521/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     >  SRR4302541.2279490/1‑94 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302541.8229010/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302541.741148/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302541.6765135/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     >  SRR4302541.1787036/1‑94 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGACCGACT                                     >  SRR4302541.8911320/1‑94 (MQ=60)
                                                       TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTG                                    <  SRR4302541.3592474/94‑1 (MQ=60)
                                                       TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATGTTCTTGTTGGGTTATCGCTGGATGGCCCGCCTG                                    >  SRR4302541.7927773/1‑94 (MQ=60)
                                                        TCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGA                                   <  SRR4302541.1968774/94‑1 (MQ=60)
                                                        TCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGA                                   >  SRR4302541.7971769/1‑94 (MQ=60)
                                                        TCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGA                                   >  SRR4302541.6111452/1‑94 (MQ=60)
                                                         CCAGCCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302541.1935336/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302541.6306869/1‑94 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302541.3170549/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302541.1617259/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302541.1266248/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302541.6705567/94‑1 (MQ=60)
                                                          CAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGA                                 >  SRR4302541.6340741/1‑94 (MQ=60)
                                                          CAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGA                                 >  SRR4302541.5909976/1‑94 (MQ=60)
                                                          CAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGA                                 <  SRR4302541.4920922/94‑1 (MQ=60)
                                                          CAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCCGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGA                                 >  SRR4302541.5152769/1‑94 (MQ=60)
                                                            GACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATC                               >  SRR4302541.2237469/1‑94 (MQ=60)
                                                            GACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATC                               >  SRR4302541.4758003/1‑94 (MQ=60)
                                                            GACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATC                               <  SRR4302541.6102841/94‑1 (MQ=60)
                                                             ACTAACGGGGTGCTGCT‑GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             <  SRR4302541.2938517/94‑1 (MQ=60)
                                                             ACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCACCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCC                              >  SRR4302541.7067690/1‑94 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302541.5406925/1‑94 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             <  SRR4302541.6624699/94‑1 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302541.2967163/1‑94 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             >  SRR4302541.2675950/1‑94 (MQ=60)
                                                                AACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACA                           >  SRR4302541.5304398/1‑94 (MQ=60)
                                                                AACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACA                           >  SRR4302541.8305088/1‑94 (MQ=60)
                                                                  CGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAAT                         >  SRR4302541.7603063/1‑94 (MQ=60)
                                                                  CGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAAT                         >  SRR4302541.2852812/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        >  SRR4302541.1113865/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATA                        >  SRR4302541.7593848/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTCTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        <  SRR4302541.7008718/94‑1 (MQ=60)
                                                                    GCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCA                       >  SRR4302541.3251874/1‑94 (MQ=60)
                                                                    GCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCA                       >  SRR4302541.5272366/1‑94 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      >  SRR4302541.12559/1‑94 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      <  SRR4302541.811777/94‑1 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      >  SRR4302541.6011308/1‑94 (MQ=60)
                                                                      GTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAT                     <  SRR4302541.5212696/94‑1 (MQ=60)
                                                                      GTGCTGCTGGATGACGAATGGTGCGCGTTTATCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAAT                     <  SRR4302541.5368594/94‑1 (MQ=60)
                                                                       TGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATA                    >  SRR4302541.2007984/1‑94 (MQ=60)
                                                                        GCTGCTGTATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302541.5427087/94‑1 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302541.4994350/94‑1 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302541.4994349/94‑1 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302541.2006161/94‑1 (MQ=60)
                                                                         GTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCAGCCTGAGATCCACAATCAATATC                  <  SRR4302541.1539459/94‑1 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCATAATCAATATC                  <  SRR4302541.6059502/94‑1 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  <  SRR4302541.4134205/94‑1 (MQ=60)
                                                                          TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302541.5957624/94‑1 (MQ=60)
                                                                          TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302541.1741810/94‑1 (MQ=60)
                                                                          TGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302541.7524214/94‑1 (MQ=60)
                                                                           GCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGC                >  SRR4302541.4166419/1‑94 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGTATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               <  SRR4302541.8570892/94‑1 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               >  SRR4302541.7401173/1‑94 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               >  SRR4302541.222261/1‑94 (MQ=60)
                                                                            CTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCG               >  SRR4302541.3507933/1‑94 (MQ=60)
                                                                             TGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACGATCAATATCGCGT              <  SRR4302541.6503613/94‑1 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302541.5763933/94‑1 (MQ=60)
                                                                               GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCCCAATCAATATCGCGTGA            >  SRR4302541.2753014/1‑94 (MQ=60)
                                                                               GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            >  SRR4302541.7373177/1‑94 (MQ=60)
                                                                                ATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAC           >  SRR4302541.3368482/1‑94 (MQ=60)
                                                                                ATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAC           >  SRR4302541.1868085/1‑94 (MQ=60)
                                                                                ATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGAC           <  SRR4302541.1326905/94‑1 (MQ=60)
                                                                                 TGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACT          <  SRR4302541.6153553/94‑1 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302541.7783268/94‑1 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302541.7542957/94‑1 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        >  SRR4302541.2639022/1‑94 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        <  SRR4302541.8887174/94‑1 (MQ=60)
                                                                                    CGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAA       <  SRR4302541.1821985/94‑1 (MQ=60)
                                                                                     GAATGGTGCGTGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302541.1409235/94‑1 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      >  SRR4302541.2389539/1‑94 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      >  SRR4302541.6128840/1‑94 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302541.1409234/94‑1 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     <  SRR4302541.8680578/94‑1 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     >  SRR4302541.204656/1‑94 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     <  SRR4302541.3403311/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   <  SRR4302541.4211935/94‑1 (MQ=60)
                                                                                         GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302541.1904066/94‑1 (MQ=60)
                                                                                             |                                                                                         
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑