Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,980,918 2 bp→CG coding (348‑349/1236 nt) aslB → putative arylsulfatase regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,980,9180GC100.0% 870.6 / NA 248W116C (TGG→TGC) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (111/137);  total (111/137)
*NC_0009133,980,9190CG99.6% 851.6 / NA 248P117A (CCG→GCG) aslBputative arylsulfatase regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (110/137);  minor base T (0/1);  total (110/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005
                                                                                       ||                                                                                      
cgtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGtt                                                                                    >  1:5678459/1‑93 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   <  2:5066239/93‑1 (MQ=255)
 gtAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttt                                                                                   >  2:1974467/1‑93 (MQ=255)
  tAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                  >  2:2376718/1‑93 (MQ=255)
  tAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtc                                                                                  <  1:2413437/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 >  1:805343/1‑93 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  1:1551221/93‑1 (MQ=255)
   aGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtct                                                                                 <  1:2873731/93‑1 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGttacac                                                                                <  1:411059/93‑5 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                >  1:506085/1‑93 (MQ=255)
    gcgcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTtctc                                                                                >  1:4957653/1‑93 (MQ=255)
      gcTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgc                                                                              <  2:5224676/93‑1 (MQ=255)
       cTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTcgcg                                                                             >  2:4782626/1‑93 (MQ=255)
         aCAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa                                                                           <  2:220150/93‑1 (MQ=255)
           aGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGc                                                                         <  1:3693100/93‑1 (MQ=255)
            ggCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGca                                                                        >  2:806323/1‑93 (MQ=255)
             gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       <  2:5850180/93‑1 (MQ=255)
             gCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcat                                                                       <  2:5437741/93‑1 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    >  1:5853365/1‑93 (MQ=255)
                aaaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGcatcat                                                                    <  2:3989315/93‑1 (MQ=255)
                 aaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                   >  1:4579435/1‑93 (MQ=255)
                 aaTATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtt                                                                   <  2:1757197/93‑1 (MQ=255)
                  atatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAttt                                                                  >  1:6159776/1‑93 (MQ=255)
                   tatGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAtttt                                                                 >  2:1328106/1‑93 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  1:3886106/93‑1 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  2:5624343/93‑1 (MQ=255)
                     tGGTGCTGGCAGGAAGATAAGTAACAGCATCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCt                                                               <  2:6208651/93‑1 (MQ=255)
                      ggTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCtt                                                              <  2:5705136/93‑1 (MQ=255)
                       gTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttg                                                             >  1:4736709/1‑93 (MQ=255)
                         gCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgtt                                                           >  1:1397949/1‑93 (MQ=255)
                          cTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgttg                                                          <  2:4685263/93‑1 (MQ=255)
                           tGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCCTTTTCTTGTTgg                                                         <  1:5152211/93‑1 (MQ=255)
                            ggCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGt                                                        <  1:5920458/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  2:1618141/93‑1 (MQ=255)
                             gCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGtt                                                       <  2:6451922/93‑1 (MQ=255)
                               atgAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     <  1:6259249/91‑1 (MQ=255)
                               aGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTa                                                     <  2:2484455/93‑1 (MQ=255)
                                 gAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATc                                                   <  1:5642807/93‑1 (MQ=255)
                                  aaGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCg                                                  >  1:3205981/1‑93 (MQ=255)
                                   aGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGc                                                 >  1:4335085/1‑93 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                <  2:2942971/93‑1 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                >  2:3121339/1‑93 (MQ=255)
                                    gATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCt                                                >  1:3717222/1‑93 (MQ=255)
                                     aaaaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTg                                               <  1:3502277/91‑1 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  2:6352542/1‑93 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  2:86927/1‑93 (MQ=255)
                                      tAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTgg                                              >  1:4192645/1‑93 (MQ=255)
                                       aaGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGa                                             >  1:3976162/1‑93 (MQ=255)
                                        aGTAACAGCTTCCGGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                            >  1:3124079/1‑93 (MQ=255)
                                        aGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAt                                            <  2:1473823/93‑1 (MQ=255)
                                        aGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATAGCTGGAt                                            <  2:1639905/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATg                                           <  1:1153907/93‑1 (MQ=255)
                                         gTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCACTTTCTTGTTGGGTTATCGCTGGATg                                           >  2:4426176/1‑93 (MQ=255)
                                           aaCAGCTTCCAGACTAACGGCGTGCAGCTGGATGACGAATGGTGCGCGGTTCTCGCGGAGCATCATATTCTTGTTGGTTTATCGCTGGATgac                                         >  1:5201511/1‑91 (MQ=255)
                                            aCAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc                                        >  2:6129941/1‑93 (MQ=255)
                                              aGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcccc                                      >  2:4985388/1‑92 (MQ=255)
                                               gCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGc                                     >  2:2816101/1‑93 (MQ=255)
                                                cTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGcc                                    <  1:5487016/93‑1 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   >  2:1136627/1‑93 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  2:1570087/93‑1 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  1:3421457/93‑1 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   >  2:1921797/1‑93 (MQ=255)
                                                 ttCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCt                                   <  2:428658/93‑1 (MQ=255)
                                                   ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTga                                 <  1:890287/93‑1 (MQ=255)
                                                   ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTga                                 >  2:3892728/1‑93 (MQ=255)
                                                   ccAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTga                                 <  1:3910132/93‑1 (MQ=255)
                                                    cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag                                >  1:6228827/1‑93 (MQ=255)
                                                    cAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTgag                                >  1:2725582/1‑93 (MQ=255)
                                                       aCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATc                             <  2:1293925/93‑1 (MQ=255)
                                                       aCTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATc                             >  2:776090/1‑93 (MQ=255)
                                                          aaCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                          >  1:1036597/1‑93 (MQ=255)
                                                          aaCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcac                          >  1:923729/1‑93 (MQ=255)
                                                           aCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcaca                         >  2:3388245/1‑93 (MQ=255)
                                                           aCGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCcaca                         <  1:1454402/93‑1 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       <  1:2126745/93‑1 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       <  1:2348542/93‑1 (MQ=255)
                                                             ggCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaat                       <  1:5742080/93‑1 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      >  1:672670/1‑93 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      >  1:1582769/1‑93 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      <  2:6425782/93‑1 (MQ=255)
                                                              gCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatc                      <  2:4562531/93‑1 (MQ=255)
                                                                gTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaatcaa                    >  1:2553270/1‑93 (MQ=255)
                                                                  gctgctGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAata                  <  1:1696327/93‑1 (MQ=255)
                                                                    tgctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGGCCGCCTGAGATCCACAATCAACATc                >  2:3400820/1‑93 (MQ=255)
                                                                     gctgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcg               >  2:6040740/1‑93 (MQ=255)
                                                                       tgGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgcg             <  2:1193690/93‑1 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:4853609/93‑1 (MQ=255)
                                                                        ggATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGt            <  1:1876659/93‑1 (MQ=255)
                                                                         gATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTg           <  2:2096741/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  2:2049929/93‑1 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       >  1:5386311/1‑93 (MQ=255)
                                                                             aCGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTa       <  2:1063561/93‑1 (MQ=255)
                                                                              cGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaa      >  1:4417799/1‑93 (MQ=255)
                                                                               gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa     <  2:5955532/93‑1 (MQ=255)
                                                                               gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa     <  2:2587451/93‑1 (MQ=255)
                                                                               gAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTCGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa     <  2:4528277/93‑1 (MQ=255)
                                                                               gAATGGGGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGCATCGTCCGCCTGAGATCCACAATCAATATCGCGTGACTaaa     >  1:4896099/1‑93 (MQ=255)
                                                                                aaTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAg    <  2:4341122/93‑1 (MQ=255)
                                                                                 aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAgg   <  2:3087844/93‑1 (MQ=255)
                                                                                 aTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAgg   <  1:5214133/93‑1 (MQ=255)
                                                                                       ||                                                                                      
CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT  >  NC_000913/3980831‑3981005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007
                                                                                             |                                                                                         
CCGTGCCGTAGCGCGACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGC                                                                                           <  SRR4302534.2908428/94‑1 (MQ=60)
     CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTT                                                                                      <  SRR4302534.5155727/94‑1 (MQ=60)
     CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTT                                                                                      >  SRR4302534.5832198/1‑94 (MQ=60)
     CCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTT                                                                                      <  SRR4302534.2779545/94‑1 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302534.2244074/94‑1 (MQ=60)
      CGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTT                                                                                     <  SRR4302534.2695801/94‑1 (MQ=60)
        TAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCT                                                                                   >  SRR4302534.5170984/1‑94 (MQ=60)
        TAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCT                                                                                   <  SRR4302534.2477621/94‑1 (MQ=60)
        TAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCT                                                                                   >  SRR4302534.825577/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302534.1591863/94‑1 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  >  SRR4302534.6402473/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302534.2482265/94‑1 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  >  SRR4302534.4458412/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  >  SRR4302534.5091910/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  >  SRR4302534.518667/1‑94 (MQ=60)
         AGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTC                                                                                  <  SRR4302534.2949190/94‑1 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                >  SRR4302534.4994130/1‑94 (MQ=60)
           CGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGC                                                                                >  SRR4302534.3743323/1‑94 (MQ=60)
             CTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGG                                                                              <  SRR4302534.2240758/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302534.1545715/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302534.3613721/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAG                                                                            <  SRR4302534.6330843/94‑1 (MQ=60)
               ACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGGTTCTCGCGGCG                                                                            >  SRR4302534.2817608/1‑94 (MQ=60)
                 AGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCA                                                                          <  SRR4302534.3792402/94‑1 (MQ=60)
                  GGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTTTCGCGGAGCAT                                                                         >  SRR4302534.1004902/1‑94 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       <  SRR4302534.3975402/94‑1 (MQ=60)
                    CGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCA                                                                       <  SRR4302534.5480821/94‑1 (MQ=60)
                     GAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCAT                                                                      >  SRR4302534.6012289/1‑94 (MQ=60)
                      AAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATT                                                                     >  SRR4302534.2450673/1‑94 (MQ=60)
                      AAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATT                                                                     >  SRR4302534.4703642/1‑94 (MQ=60)
                      AAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATT                                                                     >  SRR4302534.5014411/1‑94 (MQ=60)
                      AAATATGGTACTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGGCGAATGGTGCGCGGTTCTCGCGGCGCATCATT                                                                     >  SRR4302534.6488527/1‑94 (MQ=60)
                       AATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTT                                                                    >  SRR4302534.6327318/1‑94 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   >  SRR4302534.3856036/1‑94 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   <  SRR4302534.775009/94‑1 (MQ=60)
                        ATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTT                                                                   >  SRR4302534.1444316/1‑94 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  <  SRR4302534.5696864/94‑1 (MQ=60)
                         TATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTC                                                                  >  SRR4302534.4935167/1‑94 (MQ=60)
                          ATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCT                                                                 >  SRR4302534.3474194/1‑94 (MQ=60)
                           TGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTT                                                                <  SRR4302534.3990432/94‑1 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               >  SRR4302534.4865316/1‑94 (MQ=60)
                            GGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTG                                                               <  SRR4302534.136864/94‑1 (MQ=60)
                             GTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGT                                                              <  SRR4302534.4587084/94‑1 (MQ=60)
                              TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             <  SRR4302534.4044403/94‑1 (MQ=60)
                              TGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTT                                                             >  SRR4302534.1434732/1‑94 (MQ=60)
                                CTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGG                                                           <  SRR4302534.4367789/94‑1 (MQ=60)
                                 TGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCCTTTTCTTGTTGGT                                                          <  SRR4302534.5291191/94‑1 (MQ=60)
                                  GGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTT                                                         <  SRR4302534.6081205/94‑1 (MQ=60)
                                    CAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTA                                                       >  SRR4302534.5558617/1‑94 (MQ=60)
                                     ATGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302534.6428839/94‑1 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCTCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302534.1859242/94‑1 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      <  SRR4302534.5926758/94‑1 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302534.3286622/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302534.1594363/1‑94 (MQ=60)
                                     AGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTAT                                                      >  SRR4302534.4404686/1‑94 (MQ=60)
                                      GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302534.507510/94‑1 (MQ=60)
                                      GGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATC                                                     <  SRR4302534.5563589/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302534.5795552/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    <  SRR4302534.5962361/94‑1 (MQ=60)
                                       GAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCG                                                    >  SRR4302534.3291002/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302534.4452279/1‑94 (MQ=60)
                                        AAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGC                                                   >  SRR4302534.1329239/1‑94 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302534.3817158/1‑94 (MQ=60)
                                         AGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCT                                                  >  SRR4302534.5808194/1‑94 (MQ=60)
                                          GATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 <  SRR4302534.3094407/94‑1 (MQ=60)
                                          GATAAGTAACAGCGTCCAGACTAACGGCGGGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTG                                                 <  SRR4302534.1636093/94‑1 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTTG                                                <  SRR4302534.6377382/94‑1 (MQ=60)
                                           ATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                >  SRR4302534.4305910/1‑94 (MQ=60)
                                           AAAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGG                                                <  SRR4302534.3596483/94‑1 (MQ=60)
                                            TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGA                                               <  SRR4302534.4107857/94‑1 (MQ=60)
                                            TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGA                                               >  SRR4302534.4082571/1‑94 (MQ=60)
                                            TAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGA                                               <  SRR4302534.1674941/94‑1 (MQ=60)
                                             AAGTAACAGCTTCCGGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGAT                                              >  SRR4302534.3206213/1‑94 (MQ=60)
                                              AGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATG                                             <  SRR4302534.2654546/94‑1 (MQ=60)
                                               GTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGG                                            <  SRR4302534.1184157/94‑1 (MQ=60)
                                                TAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGC                                           >  SRR4302534.866821/1‑94 (MQ=60)
                                                TAACAGCTTCCAGACTAACGGCGTGCAGCTGGATGACGAATGGTGCGCGGTTCTCGCGGAGCATCATATTCTTGTTGGTTTATCGCTGGATGAC                                           >  SRR4302534.5341830/1‑94 (MQ=60)
                                                 AACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCC                                          >  SRR4302534.2132916/1‑94 (MQ=60)
                                                  ACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCC                                         <  SRR4302534.2084248/94‑1 (MQ=60)
                                                  ACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCC                                         <  SRR4302534.4972688/94‑1 (MQ=60)
                                                  ACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCC                                         <  SRR4302534.1137016/94‑1 (MQ=60)
                                                    AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       >  SRR4302534.904208/1‑94 (MQ=60)
                                                    AGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGC                                       <  SRR4302534.5359708/94‑1 (MQ=60)
                                                      CTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCT                                     <  SRR4302534.5634999/94‑1 (MQ=60)
                                                       TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTG                                    <  SRR4302534.3513388/94‑1 (MQ=60)
                                                       TTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTG                                    >  SRR4302534.3452732/1‑94 (MQ=60)
                                                        TCCAGACTAAGGGCGTGCTGCTGGATGACAACTGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGACTGA                                   >  SRR4302534.3185139/1‑94 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302534.5867814/1‑94 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302534.6397917/1‑94 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302534.912828/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  <  SRR4302534.4014977/94‑1 (MQ=60)
                                                         CCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAG                                  >  SRR4302534.2797480/1‑94 (MQ=60)
                                                           AGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGGTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGGCCGCCTGAGAT                                >  SRR4302534.5096564/1‑94 (MQ=60)
                                                            GACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATC                               <  SRR4302534.6583083/94‑1 (MQ=60)
                                                            GACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATC                               <  SRR4302534.2926342/94‑1 (MQ=60)
                                                              CTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCA                             <  SRR4302534.3271354/94‑1 (MQ=60)
                                                               TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAC                            >  SRR4302534.947252/1‑94 (MQ=60)
                                                               TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAC                            >  SRR4302534.5339874/1‑94 (MQ=60)
                                                               TAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAC                            >  SRR4302534.1063345/1‑94 (MQ=60)
                                                                AACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACA                           >  SRR4302534.5353998/1‑94 (MQ=60)
                                                                 ACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAA                          <  SRR4302534.1492658/94‑1 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        <  SRR4302534.2410869/94‑1 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        >  SRR4302534.689071/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        >  SRR4302534.1624167/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        <  SRR4302534.5897732/94‑1 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATC                        >  SRR4302534.1588439/1‑94 (MQ=60)
                                                                   GGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATA                        <  SRR4302534.2182753/94‑1 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      >  SRR4302534.2620962/1‑94 (MQ=60)
                                                                     CGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAA                      >  SRR4302534.3798164/1‑94 (MQ=60)
                                                                       TGCTGTTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATA                    <  SRR4302534.4666119/94‑1 (MQ=60)
                                                                       TGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATA                    >  SRR4302534.3555811/1‑94 (MQ=60)
                                                                        GCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATAT                   <  SRR4302534.1740413/94‑1 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  >  SRR4302534.3993056/1‑94 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  >  SRR4302534.6061536/1‑94 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  >  SRR4302534.929567/1‑94 (MQ=60)
                                                                         CTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATC                  >  SRR4302534.6166219/1‑94 (MQ=60)
                                                                          TGCTGTATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCG                 <  SRR4302534.6278685/94‑1 (MQ=60)
                                                                           GCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGC                >  SRR4302534.4023369/1‑94 (MQ=60)
                                                                             TGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGT              >  SRR4302534.5809846/1‑94 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302534.4985293/94‑1 (MQ=60)
                                                                              GGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTG             <  SRR4302534.1925515/94‑1 (MQ=60)
                                                                               GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            <  SRR4302534.4244623/94‑1 (MQ=60)
                                                                               GATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGA            <  SRR4302534.2300502/94‑1 (MQ=60)
                                                                                  GACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTA         >  SRR4302534.5531391/1‑94 (MQ=60)
                                                                                   ACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAA        >  SRR4302534.4537068/1‑94 (MQ=60)
                                                                                    CGAATGGGGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGCATCGTCCGCCTGAGATCCACAATCAATATCGCGTGACTAAA       >  SRR4302534.5028839/1‑94 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302534.1004338/94‑1 (MQ=60)
                                                                                     GAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAG      <  SRR4302534.5280234/94‑1 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCCTTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATACACAATCAATATCGCGTGACTAAAGG     >  SRR4302534.4828455/1‑94 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     <  SRR4302534.2554854/94‑1 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     <  SRR4302534.4119401/94‑1 (MQ=60)
                                                                                      AATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGG     >  SRR4302534.5327796/1‑94 (MQ=60)
                                                                                       ATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGT    <  SRR4302534.5354854/94‑1 (MQ=60)
                                                                                        TGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTG   >  SRR4302534.3421613/1‑94 (MQ=60)
                                                                                         GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302534.736601/94‑1 (MQ=60)
                                                                                         GGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  <  SRR4302534.3992214/94‑1 (MQ=60)
                                                                                             |                                                                                         
CCGTGCCGTAGCGCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGGCCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGG  >  NC_000913/3980825‑3981007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑