Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5191.T100.0% 815.3 / NA 229intergenic (‑268/‑530)cpxR/yiiPtranscriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (104/125);  total (104/125)

Only 100 of 288 total aligned reads displayed.

GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605
                                                                                             |                                                                                      
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  1:8118637/1‑89 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  2:720289/1‑89 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  >  1:4616562/1‑93 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 <  1:1780903/93‑1 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 >  1:4120801/1‑93 (MQ=255)
         gCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCagag                                                                                <  1:448088/93‑1 (MQ=255)
         gCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCagag                                                                                <  2:7297695/93‑1 (MQ=255)
          cTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGc                                                                               >  1:2072434/1‑93 (MQ=255)
          cTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGc                                                                               <  1:5168279/93‑1 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCTGGGTAAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              <  2:5874342/93‑1 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  2:8350884/1‑93 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              <  2:5744316/93‑1 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  2:5267246/1‑93 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              <  1:6750378/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  2:6739619/93‑1 (MQ=255)
              cagtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCata                                                                           <  2:2713071/93‑1 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          <  1:4105707/93‑1 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATg                                                                         <  1:3556436/93‑1 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGt                                                                        >  2:2388175/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGt                                                                        >  1:4913344/1‑93 (MQ=255)
                 tcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGt                                                                        <  2:7485892/93‑1 (MQ=255)
                  cagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGtt                                                                       >  2:4488822/1‑93 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      <  1:876725/93‑1 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  2:2790536/1‑93 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCg                                                                     >  1:5789588/1‑93 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    <  1:224872/93‑1 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    <  2:3798987/93‑1 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGACATATGTTCGACGGCa                                                               <  2:3289122/93‑1 (MQ=255)
                           aaGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAt                                                              >  2:1211455/1‑93 (MQ=255)
                            aGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTTGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATa                                                             >  2:790290/1‑93 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            >  1:4807732/1‑93 (MQ=255)
                              ccACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAg                                                           >  2:6002612/1‑93 (MQ=255)
                              ccACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAg                                                           >  1:4211717/1‑93 (MQ=255)
                              ccACGCTGCTGAAGTCGGTACAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAg                                                           <  1:7793218/93‑1 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGt                                                          <  2:2819515/93‑1 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGt                                                          <  2:3720337/93‑1 (MQ=255)
                               cACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCACATGTTCGACGGCATAAGt                                                          >  1:5421319/1‑93 (MQ=255)
                                  gctgctGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTa                                                       <  1:3949089/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     >  2:6449738/1‑93 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     >  1:7735664/1‑93 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAAATGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     >  1:556796/1‑93 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    <  2:635294/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    >  2:5508714/1‑93 (MQ=255)
                                      ctgATGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   >  2:5598071/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  2:548336/1‑93 (MQ=255)
                                        gAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGaa                                                 <  1:4052787/93‑1 (MQ=255)
                                        gAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGaa                                                 >  1:8521763/1‑93 (MQ=255)
                                           gTCGGTTCAGGCGATAACTGGCATCCGGGTGAATAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAt                                              >  1:8742035/1‑93 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             <  2:201695/93‑1 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           >  1:1462570/1‑93 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           <  1:3504130/93‑1 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          <  2:4366994/93‑1 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          >  2:1879495/1‑93 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          >  2:8726249/1‑93 (MQ=255)
                                                ttCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCg                                         >  1:646648/1‑93 (MQ=255)
                                                 tCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGt                                        >  1:4332440/1‑93 (MQ=255)
                                                 tCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGt                                        <  1:5672747/93‑1 (MQ=255)
                                                   aGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTca                                      <  2:6834871/93‑1 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     <  1:7161509/93‑1 (MQ=255)
                                                       gATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagcag                                  >  1:7913739/1‑93 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  1:4751884/93‑1 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  2:794178/93‑1 (MQ=255)
                                                        aTAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGa                                 <  2:3692182/93‑1 (MQ=255)
                                                          aaCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATg                               <  2:3200656/93‑1 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              <  2:6060330/93‑1 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            <  1:3277246/93‑1 (MQ=255)
                                                              ggCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgag                           >  2:6578985/1‑93 (MQ=255)
                                                               gCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          >  2:8226689/1‑93 (MQ=255)
                                                               gCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          >  2:1370919/1‑93 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  1:3039903/93‑1 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCt                       >  1:2442869/1‑93 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCt                       >  2:4688577/1‑93 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAGTTTCGACGGCTTAAGGTTAACCGTACATCAGCGTCAGCAGATGTGAGATCt                       >  2:7785339/1‑93 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  1:7360691/93‑1 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     >  1:2963910/1‑93 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTACCCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    <  2:8565989/93‑1 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    >  1:943319/1‑93 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    >  1:1234779/1‑93 (MQ=255)
                                                                     gggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAt                    <  2:3514209/93‑1 (MQ=255)
                                                                      ggTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATg                   >  1:3740025/1‑93 (MQ=255)
                                                                       gTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGc                  >  1:4572199/1‑93 (MQ=255)
                                                                        tGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCa                 <  1:8202547/93‑1 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                <  2:4185198/93‑1 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaaa               >  1:6109023/1‑92 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               >  1:8019629/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               <  2:2079023/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  1:1019087/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  1:4418640/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  1:7564358/1‑93 (MQ=255)
                                                                             aaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAgg            >  1:6703498/1‑93 (MQ=255)
                                                                              aCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGc           <  1:338448/93‑1 (MQ=255)
                                                                               cTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGcc          <  1:5381290/93‑1 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  2:5005452/93‑1 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  2:3044400/93‑1 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     >  1:6223752/1‑93 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     >  2:4041230/1‑93 (MQ=255)
                                                                                     cagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAc    <  2:6277843/93‑1 (MQ=255)
                                                                                     cagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAc    <  2:7397594/93‑1 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCCGCGTCAGCAGATGCGAGATCTTATGCACCAGGCCCGGCACGa  >  1:2946566/1‑93 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  >  1:702076/1‑93 (MQ=255)
                                                                                             |                                                                                      
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103426‑4103612
                                                                                             |                                                                                              
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                >  SRR4302541.420715/1‑94 (MQ=60)
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG                                                                                                >  SRR4302541.4041434/1‑94 (MQ=60)
 GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑T                                                                                              <  SRR4302541.2062806/94‑1 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              >  SRR4302541.930229/1‑92 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              <  SRR4302541.5770602/94‑3 (MQ=60)
  GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                              <  SRR4302541.4905331/94‑3 (MQ=60)
   CCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGtac                                                                                             <  SRR4302541.3333832/94‑4 (MQ=60)
    CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                            >  SRR4302541.8207557/1‑94 (MQ=60)
     TCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGC                                                                                           <  SRR4302541.3923580/94‑1 (MQ=60)
      CAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCA                                                                                          >  SRR4302541.1634569/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTCCGCAGCGCAGTACGCAG                                                                                         >  SRR4302541.4663478/1‑94 (MQ=60)
       AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                         >  SRR4302541.4663479/1‑94 (MQ=60)
        acgcgcgcagtgagttcgtgAAGCCACGCTGCTGAAGTCGGTGCAGGCGATGACTGGCATCCGCGGGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                        <  SRR4302541.2758725/74‑1 (MQ=58)
        ACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                        >  SRR4302541.4161497/1‑94 (MQ=60)
        ACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                        >  SRR4302541.7463679/1‑94 (MQ=60)
        ACGCCGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                        <  SRR4302541.4583827/94‑1 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302541.1798194/94‑1 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302541.4624759/94‑1 (MQ=60)
         CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTAAGCAGAG                                                                                       >  SRR4302541.7243929/1‑94 (MQ=60)
         CGCTGGCAGCCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                       <  SRR4302541.4074480/94‑1 (MQ=60)
          GCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGC                                                                                      <  SRR4302541.452348/94‑1 (MQ=60)
          GCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGC                                                                                      >  SRR4302541.2092467/1‑94 (MQ=60)
          GCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGC                                                                                      <  SRR4302541.3039519/94‑1 (MQ=60)
           CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                     <  SRR4302541.5221463/94‑1 (MQ=60)
            TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                    <  SRR4302541.6822539/94‑1 (MQ=60)
             GGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCAT                                                                                   >  SRR4302541.4444772/1‑94 (MQ=60)
              GCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATA                                                                                  <  SRR4302541.564866/94‑1 (MQ=60)
               CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                                 <  SRR4302541.6790231/94‑1 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                >  SRR4302541.6891076/1‑94 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                >  SRR4302541.5560620/1‑94 (MQ=60)
                AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                                <  SRR4302541.4146243/94‑1 (MQ=60)
                 GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               >  SRR4302541.4963493/1‑94 (MQ=60)
                 GTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGT                                                                               <  SRR4302541.3592413/94‑1 (MQ=60)
                  TCAGTTCATTAAGCCACGCTGCCGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                              <  SRR4302541.8181229/94‑1 (MQ=60)
                   TAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302541.5392997/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302541.4817449/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302541.5392995/94‑1 (MQ=60)
                   CAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                             <  SRR4302541.6888186/94‑1 (MQ=60)
                    AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            <  SRR4302541.884734/94‑1 (MQ=60)
                    AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                            >  SRR4302541.5848422/1‑94 (MQ=60)
                     GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                           <  SRR4302541.7416328/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302541.8849771/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302541.4358863/94‑1 (MQ=60)
                      TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                          <  SRR4302541.226607/94‑1 (MQ=60)
                       TCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACG                                                                         <  SRR4302541.679175/94‑1 (MQ=60)
                        CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                        <  SRR4302541.2272319/94‑1 (MQ=60)
                        CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                        <  SRR4302541.803544/94‑1 (MQ=60)
                          TTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCA                                                                      <  SRR4302541.5731357/94‑1 (MQ=60)
                           TAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAT                                                                     <  SRR4302541.8484653/94‑1 (MQ=60)
                             AGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAA                                                                   >  SRR4302541.4856764/1‑94 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  >  SRR4302541.4253611/1‑94 (MQ=60)
                              GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                                  <  SRR4302541.6560717/94‑1 (MQ=60)
                               CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302541.5476934/1‑94 (MQ=60)
                               CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 >  SRR4302541.7856475/1‑94 (MQ=60)
                               CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCACATGTTCGACGGCATAAGT                                                                 >  SRR4302541.5476936/1‑94 (MQ=60)
                               CCACGCTGCTGAAGTCGGTACAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                                 <  SRR4302541.7876783/94‑1 (MQ=60)
                                CACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGCTCGACGGCATAAGTT                                                                >  SRR4302541.7823480/1‑94 (MQ=60)
                                  CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                              >  SRR4302541.6291715/1‑94 (MQ=60)
                                   GCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAA                                                             >  SRR4302541.5707246/1‑94 (MQ=60)
                                   GCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAA                                                             <  SRR4302541.3988358/94‑1 (MQ=60)
                                   GCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAA                                                             >  SRR4302541.51277/1‑94 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            >  SRR4302541.7817963/1‑94 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            >  SRR4302541.7134287/1‑94 (MQ=60)
                                    CTGCTGAAGTCGGTTCAGGCGATAAATGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAC                                                            >  SRR4302541.562036/1‑94 (MQ=60)
                                     TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                           <  SRR4302541.3482419/94‑1 (MQ=60)
                                      GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                          <  SRR4302541.2605076/94‑1 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                         >  SRR4302541.2095349/1‑94 (MQ=60)
                                       CTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGCGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGA                                                         >  SRR4302541.8649456/1‑94 (MQ=60)
                                        TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAA                                                        >  SRR4302541.8613469/1‑94 (MQ=60)
                                         GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                       <  SRR4302541.4092779/94‑1 (MQ=60)
                                         GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                       >  SRR4302541.2989470/1‑94 (MQ=60)
                                          AAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACA                                                      <  SRR4302541.4755520/94‑1 (MQ=60)
                                           AGTCGGTTCAGGCTATAACTGGAATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     <  SRR4302541.8820750/94‑1 (MQ=60)
                                           AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAATAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     >  SRR4302541.8835600/1‑94 (MQ=60)
                                           AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                     >  SRR4302541.2149106/1‑94 (MQ=60)
                                            GTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATC                                                    <  SRR4302541.3551127/94‑1 (MQ=60)
                                             TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                   <  SRR4302541.272442/94‑1 (MQ=60)
                                             GCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                   <  SRR4302541.6216036/94‑1 (MQ=60)
                                              CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAATTTTAACCGAACATCAG                                                  >  SRR4302541.7908210/1‑94 (MQ=60)
                                              CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  >  SRR4302541.1476881/1‑94 (MQ=60)
                                              CGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAG                                                  >  SRR4302541.1476882/1‑94 (MQ=60)
                                               GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                                 <  SRR4302541.3539315/94‑1 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                <  SRR4302541.4562855/94‑1 (MQ=60)
                                                GTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCG                                                >  SRR4302541.652758/1‑94 (MQ=60)
                                                 TTCAGGCGATAACTGGCGTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGT                                               <  SRR4302541.7921857/94‑1 (MQ=60)
                                                 TTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGT                                               <  SRR4302541.928193/94‑1 (MQ=60)
                                                 TTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGT                                               >  SRR4302541.4375694/1‑94 (MQ=60)
                                                  TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                              <  SRR4302541.3699796/94‑1 (MQ=60)
                                                  TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                              >  SRR4302541.8466334/1‑94 (MQ=60)
                                                  TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                              <  SRR4302541.5730643/94‑1 (MQ=60)
                                                  TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                              <  SRR4302541.7667896/94‑1 (MQ=60)
                                                    AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                            >  SRR4302541.4340571/1‑94 (MQ=60)
                                                     GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                           <  SRR4302541.7237852/94‑1 (MQ=60)
                                                       CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                         >  SRR4302541.8000821/1‑94 (MQ=60)
                                                        GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                        >  SRR4302541.5540804/1‑94 (MQ=60)
                                                         ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                       <  SRR4302541.4800437/94‑1 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                      >  SRR4302541.6319370/1‑94 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                      <  SRR4302541.6749718/94‑1 (MQ=60)
                                                          TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                      <  SRR4302541.338011/94‑1 (MQ=60)
                                                           AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                     <  SRR4302541.4471491/94‑1 (MQ=60)
                                                           AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                     <  SRR4302541.3803632/94‑1 (MQ=60)
                                                            ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCG                                    <  SRR4302541.3522320/94‑1 (MQ=60)
                                                             CTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGA                                   <  SRR4302541.4705222/94‑1 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302541.3310297/94‑1 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302541.8213107/94‑1 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  <  SRR4302541.8284556/94‑1 (MQ=60)
                                                              TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                                  >  SRR4302541.409735/1‑94 (MQ=60)
                                                                 CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                               >  SRR4302541.3578316/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              >  SRR4302541.2467100/1‑94 (MQ=60)
                                                                  ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCT                              <  SRR4302541.3070618/94‑1 (MQ=60)
                                                                    CCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTA                            >  SRR4302541.7120781/1‑94 (MQ=60)
                                                                    CCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTA                            >  SRR4302541.2993744/1‑94 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           <  SRR4302541.7439346/94‑1 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           >  SRR4302541.952301/1‑94 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           <  SRR4302541.4456236/94‑1 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           >  SRR4302541.1246937/1‑94 (MQ=60)
                                                                     CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                           >  SRR4302541.3262866/1‑94 (MQ=60)
                                                                      GGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATG                          >  SRR4302541.3777664/1‑94 (MQ=60)
                                                                       GGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGC                         <  SRR4302541.6156474/94‑1 (MQ=60)
                                                                       GGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGC                         >  SRR4302541.4618504/1‑94 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        <  SRR4302541.803858/94‑1 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        >  SRR4302541.4518521/1‑94 (MQ=60)
                                                                        GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                        <  SRR4302541.1482931/94‑1 (MQ=60)
                                                                         TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                       <  SRR4302541.8292054/94‑1 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      >  SRR4302541.8107804/1‑94 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                      >  SRR4302541.1238225/1‑94 (MQ=60)
                                                                          GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAA                      >  SRR4302541.6171399/1‑94 (MQ=60)
                                                                           AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCaagtcctaatgaacag                     >  SRR4302541.8568054/1‑78 (MQ=60)
                                                                           AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                     >  SRR4302541.7645200/1‑94 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302541.1382704/94‑1 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302541.4462976/94‑1 (MQ=60)
                                                                            AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                    <  SRR4302541.1028503/94‑1 (MQ=60)
                                                                             GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   <  SRR4302541.2282108/94‑1 (MQ=60)
                                                                             GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                   >  SRR4302541.6773940/1‑94 (MQ=60)
                                                                               ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC                 <  SRR4302541.341480/94‑1 (MQ=60)
                                                                               ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC                 >  SRR4302541.8168105/1‑94 (MQ=60)
                                                                                CTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCC                >  SRR4302541.5202924/1‑94 (MQ=60)
                                                                                CTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCC                <  SRR4302541.5436522/94‑1 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            >  SRR4302541.6287339/1‑94 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            >  SRR4302541.3606957/1‑94 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            <  SRR4302541.4908286/94‑1 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            <  SRR4302541.7710781/94‑1 (MQ=60)
                                                                                    CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA            >  SRR4302541.5448993/1‑94 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG          <  SRR4302541.4157043/94‑1 (MQ=60)
                                                                                      CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG          <  SRR4302541.4157045/94‑1 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCCGCGTCAGCAGATGCGAGATCTTATGCACCAGGCCCGGCACGA         >  SRR4302541.2976188/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         >  SRR4302541.4181600/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         >  SRR4302541.6283401/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         >  SRR4302541.708676/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         >  SRR4302541.2976189/1‑94 (MQ=60)
                                                                                       AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA         >  SRR4302541.2050445/1‑94 (MQ=60)
                                                                                        GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA        <  SRR4302541.3171839/94‑1 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       >  SRR4302541.1951742/1‑94 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       <  SRR4302541.5239466/94‑1 (MQ=60)
                                                                                         CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC       <  SRR4302541.4069486/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      <  SRR4302541.2253207/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      <  SRR4302541.3717400/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      >  SRR4302541.2435933/1‑94 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      >  SRR4302541.8344672/1‑94 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      <  SRR4302541.4668094/94‑1 (MQ=60)
                                                                                          GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA      >  SRR4302541.4527553/1‑94 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302541.853668/94‑1 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302541.8521365/94‑1 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302541.8385611/1‑94 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     >  SRR4302541.4855331/1‑94 (MQ=60)
                                                                                           CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG     <  SRR4302541.652671/94‑1 (MQ=60)
                                                                                             T‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCCCGAACAGCCT  <  SRR4302541.8774805/94‑1 (MQ=60)
                                                                                             T‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  SRR4302541.6161600/1‑94 (MQ=60)
                                                                                             |                                                                                              
TGGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAGCCT  >  NC_000913/4103426‑4103612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑