Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,103,519 +T intergenic (‑268/‑530) cpxR ← / → yiiP transcriptional regulator in 2‑component system/putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5191.T99.5% 778.1 / ‑3.3 202intergenic (‑268/‑530)cpxR/yiiPtranscriptional regulator in 2‑component system/putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (95/106);  total (95/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

Only 100 of 233 total aligned reads displayed.

GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605
                                                                                             |                                                                                      
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       <  2:1094566/93‑3 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       <  2:1227113/93‑3 (MQ=255)
  ccTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagta                                                                                       <  1:3390688/93‑3 (MQ=255)
    tCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTAcgcagcgcagtacg                                                                                     >  2:557050/1‑89 (MQ=255)
      aaCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgca                                                                                   <  1:1097392/93‑1 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  <  1:5139721/93‑1 (MQ=255)
       aCGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcag                                                                                  >  2:2975621/1‑93 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 <  1:6150038/93‑1 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 >  1:330011/1‑93 (MQ=255)
        cGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCAGCCGGGTGAAGAACTTACGCAGCgcagtacgcaga                                                                                 <  2:3776531/93‑1 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  1:671499/1‑93 (MQ=255)
           tGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGcc                                                                              >  1:2943754/1‑93 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          <  1:1926736/93‑1 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          <  1:199281/93‑1 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          >  1:4743180/1‑93 (MQ=255)
               agtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCatat                                                                          <  1:3655954/93‑1 (MQ=255)
                gtcagtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATg                                                                         >  2:28319/1‑93 (MQ=255)
                   agtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTc                                                                      >  2:2218400/1‑93 (MQ=255)
                    gtTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCg                                                                     <  1:2659474/93‑1 (MQ=255)
                     ttCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGa                                                                    <  1:891530/93‑1 (MQ=255)
                      tCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAc                                                                   <  2:620511/93‑1 (MQ=255)
                        aTTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACgc                                                                 >  1:818674/1‑92 (MQ=255)
                         ttAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGc                                                                >  1:6024537/1‑93 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCa                                                               >  1:166948/1‑93 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCa                                                               >  2:3998322/1‑93 (MQ=255)
                          tAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCa                                                               >  2:4883165/1‑93 (MQ=255)
                           aaGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAt                                                              >  2:3345362/1‑93 (MQ=255)
                            aGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATa                                                             >  2:2768715/1‑93 (MQ=255)
                            aGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATa                                                             >  1:2435941/1‑93 (MQ=255)
                             gCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATaa                                                            >  2:4398840/1‑93 (MQ=255)
                              ccACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAg                                                           <  2:4433737/93‑1 (MQ=255)
                                aCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGtt                                                         <  2:2680856/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  2:2445425/93‑1 (MQ=255)
                                    tgctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAc                                                     <  2:1017955/93‑1 (MQ=255)
                                     gctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAAcc                                                    >  2:2326164/1‑93 (MQ=255)
                                      ctgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGTGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCg                                                   >  1:3554060/1‑93 (MQ=255)
                                       tgAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGa                                                  >  2:816050/1‑93 (MQ=255)
                                         aaGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAc                                                >  1:6203658/1‑93 (MQ=255)
                                          aGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACa                                               >  1:4690137/1‑93 (MQ=255)
                                           gTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAt                                              <  2:669031/93‑1 (MQ=255)
                                           gTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAt                                              >  1:3989215/1‑93 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             <  1:3063843/93‑1 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             <  2:3479378/93‑1 (MQ=255)
                                            tCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATc                                             <  1:5923329/93‑1 (MQ=255)
                                              ggTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAg                                           <  1:4857087/93‑1 (MQ=255)
                                               gTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGc                                          >  2:5040930/1‑93 (MQ=255)
                                                ttgaGGCGATAACTGATATCGGGGTAAAGAACTTACGCAGC‑AGT‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTc                                       <  1:4932154/90‑1 (MQ=255)
                                                 tCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGt                                        <  2:5391103/93‑1 (MQ=255)
                                                 tCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGt                                        <  1:5866718/93‑1 (MQ=255)
                                                  cAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTc                                       <  2:4473345/93‑1 (MQ=255)
                                                   aGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTca                                      <  2:6071810/93‑1 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  2:4654242/1‑93 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  1:4341414/1‑93 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     <  1:5419753/93‑1 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  2:6275999/1‑93 (MQ=255)
                                                    ggCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcag                                     >  1:1172703/1‑93 (MQ=255)
                                                     gCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagc                                    <  1:6116574/93‑1 (MQ=255)
                                                      cGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca                                   <  1:5760202/93‑1 (MQ=255)
                                                      cGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagca                                   <  2:5733823/93‑1 (MQ=255)
                                                       gATAACTGGCGTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTcagcag                                  <  2:3257712/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                <  1:1633832/93‑1 (MQ=255)
                                                         tAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAt                                >  2:5272539/1‑93 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              >  1:4162817/1‑93 (MQ=255)
                                                           aCTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGc                              >  1:1843028/1‑93 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            >  2:1426125/1‑93 (MQ=255)
                                                             tGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCga                            >  2:309068/1‑93 (MQ=255)
                                                               gCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCgaga                          <  1:4543406/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        >  1:5232721/1‑93 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  2:5167475/93‑1 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        >  1:557369/1‑93 (MQ=255)
                                                                 aTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATc                        <  2:1949005/93‑1 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCt                       <  2:340913/93‑1 (MQ=255)
                                                                  tCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCt                       >  2:529772/1‑93 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  1:5712600/93‑1 (MQ=255)
                                                                    cGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTa                     <  1:4459487/93‑1 (MQ=255)
                                                                       gTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGc                  >  2:2572279/1‑93 (MQ=255)
                                                                       gTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGc                  <  1:1495151/93‑1 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                >  1:5040698/1‑93 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                <  1:2350114/93‑1 (MQ=255)
                                                                         gaagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCaa                >  1:4112760/1‑93 (MQ=255)
                                                                          aagaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAc               <  2:1504628/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  1:4515133/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  1:766354/1‑93 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  2:2461610/1‑93 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  2:5828303/93‑1 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAGAGCCATATGGTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              >  2:2300598/1‑93 (MQ=255)
                                                                           agaaCTTACGCAGCGCAGTACGCAAAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACa              <  1:3914691/93‑1 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             >  2:5146167/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             >  2:4078428/1‑93 (MQ=255)
                                                                            gaaCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAg             <  2:1697855/93‑1 (MQ=255)
                                                                              aCTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGc           <  1:1053195/93‑1 (MQ=255)
                                                                               cTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGcc          >  1:3929308/1‑93 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  2:4626571/93‑1 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  1:2488088/93‑1 (MQ=255)
                                                                                ttACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGccc         <  2:1777200/93‑1 (MQ=255)
                                                                                    gcagcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCa     <  1:3732342/93‑1 (MQ=255)
                                                                                      agcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACg   >  2:5453829/1‑93 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  >  1:2795000/1‑93 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  <  1:4220866/93‑1 (MQ=255)
                                                                                       gcgcagtACGCAGAGCCATATGTTCGACGGCATAAGGTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGa  >  1:131868/1‑93 (MQ=255)
                                                                                             |                                                                                      
GGCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA  >  NC_000913/4103427‑4103605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG  >  NC_000913/4103428‑4103609
                                                                                           |                                                                                           
GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGta                                                                                           >  SRR4302534.6032877/1‑92 (MQ=60)
 CCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGtac                                                                                          <  SRR4302534.3481627/94‑4 (MQ=60)
  CTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACG                                                                                         <  SRR4302534.4909718/94‑1 (MQ=60)
   TCAACGCTGTCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGC                                                                                        >  SRR4302534.6491555/1‑94 (MQ=60)
   TCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGC                                                                                        >  SRR4302534.2650343/1‑94 (MQ=60)
     AACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAG                                                                                      <  SRR4302534.1125962/94‑1 (MQ=60)
      ACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                     >  SRR4302534.338489/1‑94 (MQ=60)
      ACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGA                                                                                     <  SRR4302534.5278425/94‑1 (MQ=60)
       CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                    <  SRR4302534.6317342/94‑1 (MQ=60)
       CGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAG                                                                                    <  SRR4302534.5393915/94‑1 (MQ=60)
         CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                  >  SRR4302534.3020999/1‑94 (MQ=60)
         CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                  >  SRR4302534.687868/1‑94 (MQ=60)
         CTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCC                                                                                  >  SRR4302534.5964236/1‑94 (MQ=60)
          TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGTAGAACTTACGCAGCGCAGTACGCAGAGCCT                                                                                 >  SRR4302534.2934614/1‑94 (MQ=60)
          TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                 >  SRR4302534.1954361/1‑94 (MQ=60)
          TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                 >  SRR4302534.1770631/1‑94 (MQ=60)
          TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                 <  SRR4302534.5989504/94‑1 (MQ=60)
          TGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCA                                                                                 >  SRR4302534.887985/1‑94 (MQ=60)
             CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              >  SRR4302534.4871939/1‑94 (MQ=60)
             CAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATAT                                                                              >  SRR4302534.6468726/1‑94 (MQ=60)
              AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             <  SRR4302534.1977106/94‑1 (MQ=60)
              AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             <  SRR4302534.204510/94‑1 (MQ=60)
              AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             <  SRR4302534.3754214/94‑1 (MQ=60)
              AGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATG                                                                             >  SRR4302534.4404362/1‑94 (MQ=60)
                TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           <  SRR4302534.6263087/94‑1 (MQ=60)
                TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           <  SRR4302534.6089489/94‑1 (MQ=60)
                TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           <  SRR4302534.2534418/94‑1 (MQ=60)
                TCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTT                                                                           >  SRR4302534.2301820/1‑94 (MQ=60)
                 TAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTC                                                                          >  SRR4302534.310392/1‑94 (MQ=60)
                  AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                         <  SRR4302534.5313853/94‑1 (MQ=60)
                  AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                         <  SRR4302534.247606/94‑1 (MQ=60)
                  AGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCG                                                                         >  SRR4302534.3561674/1‑94 (MQ=60)
                   GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        <  SRR4302534.3851657/94‑1 (MQ=60)
                   GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        <  SRR4302534.2729784/94‑1 (MQ=60)
                   GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        >  SRR4302534.4483924/1‑94 (MQ=60)
                   GTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGA                                                                        <  SRR4302534.4666297/94‑1 (MQ=60)
                    TTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                       <  SRR4302534.914108/94‑1 (MQ=60)
                    TTCATTAAGCCACGCTACTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAC                                                                       <  SRR4302534.4235620/94‑1 (MQ=60)
                      CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGG                                                                     <  SRR4302534.1157660/94‑1 (MQ=60)
                      CATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGC                                                                     >  SRR4302534.839255/1‑94 (MQ=60)
                       ATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGC                                                                    >  SRR4302534.6188262/1‑94 (MQ=60)
                        TTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCA                                                                   >  SRR4302534.171334/1‑94 (MQ=60)
                         TAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAT                                                                  <  SRR4302534.5677414/94‑1 (MQ=60)
                         TAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCAT                                                                  <  SRR4302534.3711693/94‑1 (MQ=60)
                          AAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATA                                                                 <  SRR4302534.1637607/94‑1 (MQ=60)
                          AAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATA                                                                 >  SRR4302534.2500689/1‑94 (MQ=60)
                            GCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAG                                                               <  SRR4302534.749253/94‑1 (MQ=60)
                             CCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGT                                                              >  SRR4302534.3631669/1‑94 (MQ=60)
                               CCGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTT                                                            <  SRR4302534.2587339/94‑1 (MQ=60)
                               ACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTT                                                            >  SRR4302534.4736405/1‑94 (MQ=60)
                                CGCTGCTGAAGTCGGTTCAGGCGATAACTGGCGTCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTA                                                           <  SRR4302534.1482400/94‑1 (MQ=60)
                                   TGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACC                                                        <  SRR4302534.4077017/94‑1 (MQ=60)
                                    GCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGTGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCG                                                       >  SRR4302534.3649638/1‑94 (MQ=60)
                                      TGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAA                                                     >  SRR4302534.4007994/1‑94 (MQ=60)
                                       GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                    >  SRR4302534.6372357/1‑94 (MQ=60)
                                       GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                    <  SRR4302534.1702207/94‑1 (MQ=60)
                                       GAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAAC                                                    <  SRR4302534.1240669/94‑1 (MQ=60)
                                        AAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACA                                                   >  SRR4302534.4333192/1‑94 (MQ=60)
                                        AAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACA                                                   <  SRR4302534.5910475/94‑1 (MQ=60)
                                        AAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACA                                                   >  SRR4302534.4817449/1‑94 (MQ=60)
                                         AGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACAT                                                  >  SRR4302534.4095942/1‑94 (MQ=60)
                                           TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                <  SRR4302534.3144260/94‑1 (MQ=60)
                                           TCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCA                                                <  SRR4302534.6084162/94‑1 (MQ=60)
                                             GGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGC                                              <  SRR4302534.4988861/94‑1 (MQ=60)
                                               TTGAGGCGATAACTGATATCGGGGTAAAGAACTTACGCA‑‑GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                          <  SRR4302534.5065833/94‑1 (MQ=60)
                                                TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATACAGTTAACCGAACATCAGCGTC                                           >  SRR4302534.1020959/1‑94 (MQ=60)
                                                TCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTC                                           <  SRR4302534.6026011/94‑1 (MQ=60)
                                                 CAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCA                                          >  SRR4302534.4587070/1‑94 (MQ=60)
                                                  AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                         >  SRR4302534.5198853/1‑94 (MQ=60)
                                                  AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                         >  SRR4302534.4458706/1‑94 (MQ=60)
                                                  AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                         <  SRR4302534.6361866/94‑1 (MQ=60)
                                                  AGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAG                                         >  SRR4302534.1203532/1‑94 (MQ=60)
                                                   GGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGC                                        <  SRR4302534.5565588/94‑1 (MQ=60)
                                                    GCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCA                                       <  SRR4302534.6282963/94‑1 (MQ=60)
                                                    GCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCA                                       <  SRR4302534.3108329/94‑1 (MQ=60)
                                                    GCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCA                                       >  SRR4302534.814203/1‑94 (MQ=60)
                                                     CGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAG                                      <  SRR4302534.5916465/94‑1 (MQ=60)
                                                      GATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGA                                     >  SRR4302534.4123533/1‑94 (MQ=60)
                                                       ATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGAT                                    <  SRR4302534.5860581/94‑1 (MQ=60)
                                                        TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302534.1676426/94‑1 (MQ=60)
                                                        TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302534.5144366/94‑1 (MQ=60)
                                                        TAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTCCGACGGCATAGGTTTAACCGAACATCAGCGTCAGCAGATG                                   <  SRR4302534.3279068/94‑1 (MQ=60)
                                                         AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                  >  SRR4302534.1890927/1‑94 (MQ=60)
                                                         AACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGC                                  >  SRR4302534.4275258/1‑94 (MQ=60)
                                                          ACTGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCAAACATCAGCGTCAGCAGATGCG                                 <  SRR4302534.6315712/94‑1 (MQ=60)
                                                            TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                               <  SRR4302534.1480720/94‑1 (MQ=60)
                                                            TGGCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAG                               >  SRR4302534.4917931/1‑94 (MQ=60)
                                                              GCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAT                             <  SRR4302534.5688011/94‑1 (MQ=60)
                                                              GCATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGAT                             <  SRR4302534.4666568/94‑1 (MQ=60)
                                                               CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                            >  SRR4302534.571109/1‑94 (MQ=60)
                                                               CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                            >  SRR4302534.5373995/1‑94 (MQ=60)
                                                               CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                            >  SRR4302534.1122969/1‑94 (MQ=60)
                                                               CATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATC                            <  SRR4302534.4835132/94‑1 (MQ=60)
                                                                ATCCGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCC                           <  SRR4302534.3807535/94‑1 (MQ=60)
                                                                   CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                        >  SRR4302534.6224786/1‑94 (MQ=60)
                                                                   CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                        <  SRR4302534.4580058/94‑1 (MQ=60)
                                                                   CGGGTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTAT                        <  SRR4302534.5867367/94‑1 (MQ=60)
                                                                      GTGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCA                     <  SRR4302534.1534488/94‑1 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGAGGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.6441097/1‑94 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.5176831/1‑94 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.4223397/1‑94 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.2197163/1‑94 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.2005862/1‑94 (MQ=60)
                                                                       TGAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAA                    >  SRR4302534.5056505/1‑94 (MQ=60)
                                                                        GAAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAAC                   <  SRR4302534.2412485/94‑1 (MQ=60)
                                                                         AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                  >  SRR4302534.785400/1‑94 (MQ=60)
                                                                         AAGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACA                  <  SRR4302534.2742917/94‑1 (MQ=60)
                                                                          AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                 <  SRR4302534.4637444/94‑1 (MQ=60)
                                                                          AGAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                 <  SRR4302534.2626080/94‑1 (MQ=60)
                                                                          AGAACTTACGCAGCGCAGTACGCAAAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAG                 <  SRR4302534.4019646/94‑1 (MQ=60)
                                                                           GAACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGG                <  SRR4302534.5242765/94‑1 (MQ=60)
                                                                            AACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGC               <  SRR4302534.4103498/94‑1 (MQ=60)
                                                                             ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC              <  SRR4302534.1080460/94‑1 (MQ=60)
                                                                             ACTTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCC              >  SRR4302534.4034564/1‑94 (MQ=60)
                                                                               TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCG            <  SRR4302534.1821899/94‑1 (MQ=60)
                                                                               TTACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCG            <  SRR4302534.2554185/94‑1 (MQ=60)
                                                                                TACGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGG           <  SRR4302534.4021618/94‑1 (MQ=60)
                                                                                  CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA         >  SRR4302534.880032/1‑94 (MQ=60)
                                                                                  CGCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCA         >  SRR4302534.441054/1‑94 (MQ=60)
                                                                                   GCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAC        >  SRR4302534.4660215/1‑94 (MQ=60)
                                                                                   GCAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCAC        <  SRR4302534.3832629/94‑1 (MQ=60)
                                                                                    CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       <  SRR4302534.29462/94‑1 (MQ=60)
                                                                                    CAGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACG       <  SRR4302534.340837/94‑1 (MQ=60)
                                                                                     AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA      >  SRR4302534.2868515/1‑94 (MQ=60)
                                                                                     AGCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGGTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGA      >  SRR4302534.135349/1‑94 (MQ=60)
                                                                                      GCGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAA     <  SRR4302534.4334651/94‑1 (MQ=60)
                                                                                       CGCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAAC    >  SRR4302534.6604170/1‑94 (MQ=60)
                                                                                        GCAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACA   >  SRR4302534.3193159/1‑94 (MQ=60)
                                                                                         CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGCACAG  >  SRR4302534.1425839/1‑94 (MQ=60)
                                                                                         CAGTACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG  >  SRR4302534.3566669/1‑94 (MQ=60)
                                                                                           |                                                                                           
GCCTCAACGCTGGCAGTCAGTTCATTAAGCCACGCTGCTGAAGTCGGTTCAGGCGATAACTGGCATCCGGGTGAAGAACTTACGCAGCGCAG‑ACGCAGAGCCATATGTTCGACGGCATAAGTTTAACCGAACATCAGCGTCAGCAGATGCGAGATCTTATGCAACAGGCCCGGCACGAACAG  >  NC_000913/4103428‑4103609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑