Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,114,335 T→C A156A (GCA→GCG)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,3350TC99.6% 747.9 / ‑5.5 222A156A (GCA→GCG) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (107/114);  total (108/114)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 235 total aligned reads displayed.

GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT  >  NC_000913/4114245‑4114424
                                                                                          |                                                                                         
gTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACgcgc                                                                                         >  1:6807956/1‑93 (MQ=255)
 ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                        >  1:8124104/1‑93 (MQ=255)
 ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                        >  2:3658317/1‑93 (MQ=255)
 ttGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCa                                                                                        >  1:23929/1‑93 (MQ=255)
   tgtgtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACg                                                                                      <  2:6950195/92‑1 (MQ=255)
    gtgtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGc                                                                                     <  1:8454713/93‑1 (MQ=255)
      gtAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTc                                                                                   >  1:2146514/1‑93 (MQ=255)
       tAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcg                                                                                  <  2:4917316/93‑1 (MQ=255)
         aTCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTcgcg                                                                                <  2:3072514/93‑1 (MQ=255)
          tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCga                                                                               >  1:1076315/1‑93 (MQ=255)
          tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCga                                                                               <  2:1172176/93‑1 (MQ=255)
          tCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCga                                                                               <  2:4833415/93‑1 (MQ=255)
           cGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCgag                                                                              <  2:4510918/93‑1 (MQ=255)
            ggTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCgaga                                                                             >  2:8536836/1‑93 (MQ=255)
             gTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGagag                                                                            >  2:3771067/1‑93 (MQ=255)
                 caTGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCtt                                                                        <  1:8694530/93‑1 (MQ=255)
                  aTGGACACGGCCCTGAGTCCTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTc                                                                       >  2:7949724/1‑93 (MQ=255)
                    ggACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGCGCCTTCca                                                                     >  1:7717509/1‑93 (MQ=255)
                     gACACGGCCCTGAGTCATTTTCCAGGTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcac                                                                    >  1:16970/1‑93 (MQ=255)
                     gACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcac                                                                    <  1:2403538/93‑1 (MQ=255)
                      gcacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                   <  1:1884022/92‑1 (MQ=255)
                      acacGGCCCTGAGTCATTTTCCAGATAATCCACGTATCAACCGTACAAAACAGCAATCCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                   <  2:5512690/93‑1 (MQ=255)
                      acacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCcaca                                                                   <  1:3959939/93‑1 (MQ=255)
                       cacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAt                                                                  <  1:3660062/93‑1 (MQ=255)
                       cacGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAt                                                                  <  2:246334/93‑1 (MQ=255)
                         cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                >  1:1185187/1‑93 (MQ=255)
                         cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                <  1:2955257/93‑1 (MQ=255)
                         cGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATgg                                                                <  1:4627146/93‑1 (MQ=255)
                          ggCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGt                                                               >  1:1640277/1‑93 (MQ=255)
                          ggCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGt                                                               <  2:7374245/93‑1 (MQ=255)
                           gCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTc                                                              <  1:8177550/93‑1 (MQ=255)
                            cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCg                                                             <  2:4727067/93‑1 (MQ=255)
                            cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCg                                                             >  2:4358902/1‑93 (MQ=255)
                            cccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACACGGTCg                                                             <  1:4275191/93‑1 (MQ=255)
                             ccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGa                                                            >  1:5504658/1‑93 (MQ=255)
                             ccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGa                                                            <  2:8140612/93‑1 (MQ=255)
                             ccTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGa                                                            >  2:1626329/1‑93 (MQ=255)
                              cTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                           >  2:6318909/1‑93 (MQ=255)
                              cTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                           >  1:811255/1‑93 (MQ=255)
                              cTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAg                                                           >  1:7766303/1‑93 (MQ=255)
                               tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCTCGAGAGCCTTCCACATGGTCGAgg                                                          >  1:2258056/1‑93 (MQ=255)
                               tGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAgg                                                          <  2:8704724/93‑1 (MQ=255)
                                 aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  1:3194202/93‑1 (MQ=255)
                                 aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  1:5206590/93‑1 (MQ=255)
                                 aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  2:5655677/93‑1 (MQ=255)
                                 aGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGAt                                                        <  2:7561183/93‑1 (MQ=255)
                                  gTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATc                                                       >  2:3967811/1‑93 (MQ=255)
                                   tCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATcc                                                      <  1:2845466/93‑1 (MQ=255)
                                     aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                    >  2:3879535/1‑93 (MQ=255)
                                     aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                    >  1:5680493/1‑93 (MQ=255)
                                     aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                    >  2:1602282/1‑93 (MQ=255)
                                     aTTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcac                                                    <  2:793835/93‑1 (MQ=255)
                                      ttttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcact                                                   <  2:2548391/93‑1 (MQ=255)
                                       tttCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcactt                                                  >  2:5206150/1‑93 (MQ=255)
                                            aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             >  1:4712154/1‑93 (MQ=255)
                                            aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             <  2:5377703/93‑1 (MQ=255)
                                            aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCcacttcactt                                             <  1:4409763/93‑1 (MQ=255)
                                            aGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGCATCcacttcactt                                             >  1:7496992/1‑93 (MQ=255)
                                             gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                            >  2:5885541/1‑93 (MQ=255)
                                             gATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACttt                                            <  1:1431532/93‑1 (MQ=255)
                                              aTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTg                                           >  2:8183123/1‑93 (MQ=255)
                                                  gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                       >  1:3605605/1‑93 (MQ=255)
                                                  gCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGc                                       >  1:6411020/1‑93 (MQ=255)
                                                   ccACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGcc                                      >  1:6015069/1‑93 (MQ=255)
                                                    cACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCa                                     <  2:1535225/93‑1 (MQ=255)
                                                       gTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaa                                  >  1:7433462/1‑93 (MQ=255)
                                                        tATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaa                                 >  2:8056621/1‑93 (MQ=255)
                                                        tATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaa                                 <  1:7612287/93‑1 (MQ=255)
                                                        tATCAACCGTACCAAACAGCAATTCAACCCGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaa                                 >  1:6993236/1‑93 (MQ=255)
                                                         aTCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaaa                                >  2:7225886/1‑93 (MQ=255)
                                                         aTCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGaaaa                                <  2:6037153/93‑1 (MQ=255)
                                                          tCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAg                               >  2:8422527/1‑93 (MQ=255)
                                                            aaCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTa                             >  1:7209706/1‑93 (MQ=255)
                                                             aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                            <  1:6257328/93‑1 (MQ=255)
                                                             aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTAc                            >  2:6850615/1‑93 (MQ=255)
                                                             aCCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACCTCACTTTGGTGCCAGAAAAGTAc                            <  1:917874/93‑1 (MQ=255)
                                                              ccGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACg                           <  2:1381576/93‑1 (MQ=255)
                                                               cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                          <  2:340816/93‑1 (MQ=255)
                                                               cGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACgg                          <  2:7792512/93‑1 (MQ=255)
                                                                gTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                         <  2:6935336/93‑1 (MQ=255)
                                                                gTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACggg                         <  2:8702664/93‑1 (MQ=255)
                                                                 tACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCTAGTAAAGTACgggc                        >  1:1748458/1‑92 (MQ=255)
                                                                 tACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGt                        >  2:829034/1‑93 (MQ=255)
                                                                  aCCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTc                       <  2:1956974/93‑1 (MQ=255)
                                                                     aaaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAt                    >  1:4892446/1‑93 (MQ=255)
                                                                      aaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATc                   <  1:4318294/93‑1 (MQ=255)
                                                                      aaCAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATc                   >  2:3562145/1‑93 (MQ=255)
                                                                        cAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAc                 <  1:5837379/93‑1 (MQ=255)
                                                                          gCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCa               <  2:2164705/93‑1 (MQ=255)
                                                                             aTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            <  2:6744819/93‑1 (MQ=255)
                                                                             aTTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAc            <  1:6109261/93‑1 (MQ=255)
                                                                              ttCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAcc           <  2:202859/93‑1 (MQ=255)
                                                                                caccacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCgg         <  1:3439428/93‑1 (MQ=255)
                                                                                   cacGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCGCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAt      >  1:8601014/1‑93 (MQ=255)
                                                                                    acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     >  2:5177350/1‑93 (MQ=255)
                                                                                    acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     >  1:704904/1‑93 (MQ=255)
                                                                                    acgacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAtt     <  2:5733274/93‑1 (MQ=255)
                                                                                      gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   <  2:5049322/93‑1 (MQ=255)
                                                                                      gacgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgc   <  1:38211/93‑1 (MQ=255)
                                                                                       acgCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATtgct  >  1:2545327/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT  >  NC_000913/4114245‑4114424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4114243‑4114428
                                                                                            |                                                                                             
GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCG                                                                                              <  SRR4302541.7983195/94‑1 (MQ=60)
GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCG                                                                                              <  SRR4302541.809832/94‑1 (MQ=60)
 CGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGC                                                                                             <  SRR4302541.6549217/94‑1 (MQ=60)
 CGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGC                                                                                             >  SRR4302541.6880767/1‑94 (MQ=60)
 CGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGC                                                                                             >  SRR4302541.6880768/1‑94 (MQ=60)
  GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCA                                                                                            >  SRR4302541.623846/1‑94 (MQ=60)
  GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCA                                                                                            >  SRR4302541.2067059/1‑94 (MQ=60)
  GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCA                                                                                            >  SRR4302541.24120/1‑94 (MQ=60)
  GTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCA                                                                                            >  SRR4302541.8213070/1‑94 (MQ=60)
     GGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGC                                                                                         <  SRR4302541.5329041/94‑1 (MQ=60)
      GTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCT                                                                                        <  SRR4302541.8545955/94‑1 (MQ=60)
      GTATAAGCGGTCACATGGACACGGCTCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCT                                                                                        <  SRR4302541.4393932/94‑1 (MQ=60)
       TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTC                                                                                       >  SRR4302541.2167280/1‑94 (MQ=60)
       TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTC                                                                                       <  SRR4302541.4320803/94‑1 (MQ=60)
       TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTC                                                                                       <  SRR4302541.7812078/94‑1 (MQ=60)
       TGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTC                                                                                       >  SRR4302541.5733163/1‑94 (MQ=60)
        GTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCG                                                                                      <  SRR4302541.2924769/94‑1 (MQ=60)
           ATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGA                                                                                   >  SRR4302541.1086345/1‑94 (MQ=60)
                   CATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTC                                                                           <  SRR4302541.8787595/94‑1 (MQ=60)
                    ATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCC                                                                          >  SRR4302541.3425242/1‑94 (MQ=60)
                     TGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGCGCCTTCCA                                                                         >  SRR4302541.7799595/1‑94 (MQ=60)
                     TGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCA                                                                         <  SRR4302541.8044121/94‑1 (MQ=60)
                      GGACACGGCCCTGAGTCATTTTCCAGGTAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCAC                                                                        >  SRR4302541.17109/1‑94 (MQ=60)
                      GGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCAC                                                                        >  SRR4302541.466140/1‑94 (MQ=60)
                      GGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGCATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCAC                                                                        >  SRR4302541.2380667/1‑94 (MQ=60)
                       GACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACA                                                                       >  SRR4302541.4697226/1‑94 (MQ=60)
                       GACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACA                                                                       <  SRR4302541.2427135/94‑1 (MQ=60)
                        GCACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAT                                                                      <  SRR4302541.1902118/94‑1 (MQ=60)
                        ACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACAT                                                                      <  SRR4302541.3999279/94‑1 (MQ=60)
                         CACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATG                                                                     >  SRR4302541.766539/1‑94 (MQ=60)
                         CACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATG                                                                     <  SRR4302541.3697202/94‑1 (MQ=60)
                          ACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGG                                                                    >  SRR4302541.1196646/1‑94 (MQ=60)
                          ACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGG                                                                    <  SRR4302541.8521291/94‑1 (MQ=60)
                           CGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGT                                                                   <  SRR4302541.4674214/94‑1 (MQ=60)
                           CGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGT                                                                   >  SRR4302541.1656501/1‑94 (MQ=60)
                           CGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGT                                                                   <  SRR4302541.2984964/94‑1 (MQ=60)
                             GCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCG                                                                 <  SRR4302541.8266832/94‑1 (MQ=60)
                              CCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGA                                                                >  SRR4302541.5561039/1‑94 (MQ=60)
                              CCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACACGGTCGA                                                                <  SRR4302541.4317900/94‑1 (MQ=60)
                               CCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAG                                                               >  SRR4302541.818678/1‑94 (MQ=60)
                               CCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAG                                                               <  SRR4302541.1649925/94‑1 (MQ=60)
                               CCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAG                                                               >  SRR4302541.7848923/1‑94 (MQ=60)
                                CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCTCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302541.2279861/1‑94 (MQ=60)
                                CTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGG                                                              >  SRR4302541.4033887/1‑94 (MQ=60)
                                   AGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATC                                                           <  SRR4302541.3643030/94‑1 (MQ=60)
                                   AGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATC                                                           <  SRR4302541.5260230/94‑1 (MQ=60)
                                   AGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATC                                                           <  SRR4302541.3226469/94‑1 (MQ=60)
                                     TCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCA                                                         >  SRR4302541.7092224/1‑94 (MQ=60)
                                     TCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCA                                                         <  SRR4302541.2874078/94‑1 (MQ=60)
                                      CATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCAC                                                        >  SRR4302541.5738450/1‑94 (MQ=60)
                                       ATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACT                                                       >  SRR4302541.623707/1‑94 (MQ=60)
                                         TTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTC                                                     <  SRR4302541.8807739/94‑1 (MQ=60)
                                          TTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCA                                                    <  SRR4302541.4249384/94‑1 (MQ=60)
                                          TACCAGATAAGCCGCGTATCAGCCGTTCCAAACGCCAATTCACCGCGACGCGCGCGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCA                                                    <  SRR4302541.5415367/94‑1 (MQ=60)
                                           TCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCAC                                                   >  SRR4302541.3883513/1‑94 (MQ=60)
                                             CAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTT                                                 >  SRR4302541.4760189/1‑94 (MQ=60)
                                             CAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGCATCCACTTCACTT                                                 >  SRR4302541.7577140/1‑94 (MQ=60)
                                              AGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTT                                                >  SRR4302541.1661228/1‑94 (MQ=60)
                                              AGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTT                                                <  SRR4302541.4453940/94‑1 (MQ=60)
                                              AGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTT                                                >  SRR4302541.319282/1‑94 (MQ=60)
                                               GATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTG                                               <  SRR4302541.827790/94‑1 (MQ=60)
                                               GATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTG                                               <  SRR4302541.1445453/94‑1 (MQ=60)
                                                ATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGG                                              >  SRR4302541.6066818/1‑94 (MQ=60)
                                                ATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGG                                              <  SRR4302541.1719202/94‑1 (MQ=60)
                                                 TAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGT                                             >  SRR4302541.3830901/1‑94 (MQ=60)
                                                 TAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGT                                             >  SRR4302541.2281514/1‑94 (MQ=60)
                                                  AAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTG                                            <  SRR4302541.2184609/94‑1 (MQ=60)
                                                  AAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTC                                            >  SRR4302541.3353681/1‑94 (MQ=60)
                                                   AGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302541.6476405/1‑94 (MQ=60)
                                                   AGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302541.3642152/1‑94 (MQ=60)
                                                   AGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302541.5157638/1‑94 (MQ=60)
                                                   AGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGC                                           >  SRR4302541.8734245/1‑94 (MQ=60)
                                                   AGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACGTCACTTTGGTGC                                           >  SRR4302541.8214701/1‑94 (MQ=60)
                                                    GCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCC                                          >  SRR4302541.4898191/1‑94 (MQ=60)
                                                    GCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCC                                          >  SRR4302541.6076707/1‑94 (MQ=60)
                                                     CCACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCA                                         >  SRR4302541.4674208/1‑94 (MQ=60)
                                                      CACGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAG                                        >  SRR4302541.74812/1‑94 (MQ=60)
                                                       CCGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGA                                       <  SRR4302541.2706389/94‑1 (MQ=60)
                                                       CCGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGA                                       <  SRR4302541.1838563/94‑1 (MQ=60)
                                                        CGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAA                                      <  SRR4302541.7194952/94‑1 (MQ=60)
                                                        CGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAA                                      >  SRR4302541.5814429/1‑94 (MQ=60)
                                                        CGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAA                                      >  SRR4302541.7512930/1‑94 (MQ=60)
                                                        CGTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAA                                      >  SRR4302541.8813080/1‑94 (MQ=60)
                                                         GTATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAA                                     >  SRR4302541.7059209/1‑94 (MQ=60)
                                                         GTATCAACCGTACCAAACAGCAATTCAACCCGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAA                                     >  SRR4302541.7067685/1‑94 (MQ=60)
                                                          TATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAA                                    <  SRR4302541.7693626/94‑1 (MQ=60)
                                                           ATCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAG                                   <  SRR4302541.1099936/94‑1 (MQ=60)
                                                            TCAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGT                                  >  SRR4302541.3631891/1‑94 (MQ=60)
                                                             CAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTA                                 >  SRR4302541.7286611/1‑94 (MQ=60)
                                                             CAACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTA                                 >  SRR4302541.5742709/1‑94 (MQ=60)
                                                               ACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302541.6321241/94‑1 (MQ=60)
                                                               ACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302541.261297/94‑1 (MQ=60)
                                                               ACCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACCTCACTTTGGTGCCAGAAAAGTACG                               <  SRR4302541.926458/94‑1 (MQ=60)
                                                                CCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGG                              >  SRR4302541.2418556/1‑94 (MQ=60)
                                                                CCGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGG                              <  SRR4302541.6727474/94‑1 (MQ=60)
                                                                 CGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGG                             >  SRR4302541.5097734/1‑94 (MQ=60)
                                                                 CGTACCAAACAGCAATTCACCACGACGCGCACGCTCGCCAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGG                             <  SRR4302541.7722279/94‑1 (MQ=60)
                                                                  GTACCAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCTAGTAAAGTACGGGC                            >  SRR4302541.1765522/1‑94 (MQ=60)
                                                                      CANACAGNANATCACCACGACNCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAT                        <  SRR4302541.6107088/94‑1 (MQ=60)
                                                                      CAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAT                        >  SRR4302541.4942363/1‑94 (MQ=60)
                                                                      CAAACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAAT                        >  SRR4302541.6392449/1‑94 (MQ=60)
                                                                        AACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      >  SRR4302541.7589471/1‑94 (MQ=60)
                                                                        AACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      <  SRR4302541.1484371/94‑1 (MQ=60)
                                                                        AACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCA                      <  SRR4302541.4361408/94‑1 (MQ=60)
                                                                         ACAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCAC                     <  SRR4302541.2483691/94‑1 (MQ=60)
                                                                          CAGCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACC                    <  SRR4302541.5896731/94‑1 (MQ=60)
                                                                            GCAATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAG                  <  SRR4302541.1440681/94‑1 (MQ=60)
                                                                              AATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGAC                <  SRR4302541.1864514/94‑1 (MQ=60)
                                                                               ATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               <  SRR4302541.7391421/94‑1 (MQ=60)
                                                                               ATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               <  SRR4302541.6171641/94‑1 (MQ=60)
                                                                               ATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               <  SRR4302541.8021817/94‑1 (MQ=60)
                                                                               ATTCACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACC               <  SRR4302541.8587844/94‑1 (MQ=60)
                                                                                  CACCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGT            <  SRR4302541.3473798/94‑1 (MQ=60)
                                                                                   ACCACGACGCGCACGCTCGCGAGAGCCTTCAACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTA           <  SRR4302541.7569684/94‑1 (MQ=60)
                                                                                    CCACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCGCTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTAT          >  SRR4302541.8693285/1‑94 (MQ=60)
                                                                                     CACGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATT         >  SRR4302541.711531/1‑94 (MQ=60)
                                                                                      CCGACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTG        <  SRR4302541.3850647/94‑1 (MQ=60)
                                                                                        GACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT      >  SRR4302541.2570866/1‑94 (MQ=60)
                                                                                        GACGCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCT      <  SRR4302541.38504/94‑1 (MQ=60)
                                                                                           GCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG   <  SRR4302541.2143101/94‑1 (MQ=60)
                                                                                           GCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG   <  SRR4302541.588427/94‑1 (MQ=60)
                                                                                           GCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG   >  SRR4302541.5285916/1‑94 (MQ=60)
                                                                                           GCGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCG   >  SRR4302541.5394190/1‑94 (MQ=60)
                                                                                            CGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  SRR4302541.5416154/1‑94 (MQ=60)
                                                                                            CGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  SRR4302541.2847598/1‑94 (MQ=60)
                                                                                            CGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  SRR4302541.5604531/1‑94 (MQ=60)
                                                                                            |                                                                                             
GCGTTGGTGTAATCGGTCACATGGACACGGCCCTGAGTCATTTTCCAGATAAGCCACGTATCAACCGTACCAAACAGCAATTCACCACGACGTGCACGCTCGCGAGAGCCTTCCACATGGTCGAGGATCCACTTCACTTTGGTGCCAGAAAAGTACGGGTCAATCACCAGACCGGTATTGCTGCGG  >  NC_000913/4114243‑4114428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑