Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,175,949	+G	intergenic (+82/‑76)	nusG → / → rplK	NusG/50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,175,947	1	.	G	99.6%	799.1 / ‑4.0	234	intergenic (+80/‑78)	nusG/rplK	NusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (127/106); total (127/107)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 268 total aligned reads displayed.

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAt                                                                                           <  2:3606053/93‑1 (MQ=255)

tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAt                                                                                           >  2:7582194/1‑93 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑g                                                                                         >  2:8733764/1‑93 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑g                                                                                         >  2:6395267/1‑93 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑g                                                                                         <  1:1459659/93‑1 (MQ=255)

 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑g                                                                                         <  1:2964595/93‑1 (MQ=255)

  aaaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑gg                                                                                        <  1:8271751/93‑1 (MQ=255)

    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑gggc                                                                                      >  1:3884491/1‑91 (MQ=255)

     aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑gggcc                                                                                     <  1:4100819/93‑4 (MQ=255)

      aGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑gggcct                                                                                    <  2:8373018/93‑5 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGcc                                                                                >  1:6455502/1‑93 (MQ=255)

          tAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGcc                                                                                <  2:1405706/93‑1 (MQ=255)

           aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGccc                                                                               >  2:1572847/1‑93 (MQ=255)

             cccAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGt                                                                             >  1:1482017/1‑93 (MQ=255)

              ccAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTa                                                                            >  2:577375/1‑93 (MQ=255)

               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTaa                                                                           >  1:8660378/1‑93 (MQ=255)

               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTaa                                                                           <  2:2837027/93‑1 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTaaaa                                                                         >  2:7767559/1‑93 (MQ=255)

                 gCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTaaaa                                                                         >  2:3271368/1‑93 (MQ=255)

                  cGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAAc                                                                        <  2:1668810/93‑1 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACg                                                                       >  2:6156807/1‑93 (MQ=255)

                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACg                                                                       <  2:6840953/93‑1 (MQ=255)

                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACgt                                                                      >  1:7621304/1‑92 (MQ=255)

                     tCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGAt                                                                     >  1:3059611/1‑93 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTta                                                               <  1:8180202/93‑1 (MQ=255)

                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTta                                                               <  1:7309970/93‑1 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTTTGGG‑CCTTGCCCGTAAAACGATTTTTtat                                                              <  2:856818/93‑1 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTtat                                                              >  1:4304072/1‑93 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTtat                                                              >  2:7887468/1‑93 (MQ=255)

                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTtat                                                              >  1:6468351/1‑93 (MQ=255)

                            aGCGGCGATTTAATCGTCGCACAAGGCGTGAGATTGGAATCCAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATATTTtat                                                              <  1:5506267/93‑1 (MQ=255)

                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCTTAAAACGATTTTTtata                                                             <  2:469562/93‑1 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTatat                                                            <  2:6929849/93‑1 (MQ=255)

                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTatat                                                            <  2:1815715/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATc                                                           >  1:4405396/1‑93 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATc                                                           <  2:4985976/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATc                                                           <  1:3943131/93‑1 (MQ=255)

                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATc                                                           <  1:6201416/93‑1 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCa                                                          <  1:2166213/93‑1 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCa                                                          <  2:5173087/93‑1 (MQ=255)

                                gcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCa                                                          >  2:8094580/1‑93 (MQ=255)

                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCAc                                                         >  1:7538579/1‑93 (MQ=255)

                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCAc                                                         >  1:5861865/1‑93 (MQ=255)

                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACg                                                        >  2:8468903/1‑93 (MQ=255)

                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACgg                                                       <  1:3814914/93‑1 (MQ=255)

                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACgggg                                                     >  1:5285721/1‑93 (MQ=255)

                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACgggg                                                     >  2:8180781/1‑93 (MQ=255)

                                      tAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACTGGGa                                                    >  2:1188745/1‑93 (MQ=255)

                                      tAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGa                                                    <  2:5630300/93‑1 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  >  2:4206227/1‑93 (MQ=255)

                                        aTCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGc                                                  >  1:6701526/1‑93 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 <  2:4421367/93‑1 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 >  1:1187379/1‑93 (MQ=255)

                                         tCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGcc                                                 >  2:2064578/1‑93 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                                >  2:5998069/1‑93 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                                <  2:256183/93‑1 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                                >  1:1106091/1‑93 (MQ=255)

                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCct                                                >  1:1733229/1‑93 (MQ=255)

                                           gTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctc                                               >  1:5664751/1‑93 (MQ=255)

                                            ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                              <  2:8030006/93‑1 (MQ=255)

                                            ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCctct                                              <  1:6539436/93‑1 (MQ=255)

                                             tGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCtctc                                             <  2:31380/93‑1 (MQ=255)

                                              gCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCa                                            <  1:5776682/93‑1 (MQ=255)

                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCaga                                          >  2:4060863/1‑93 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCTCGTAAAACGATTTTTTATATCACGGGAAGACTCTCAGAgg                                        >  1:2589981/1‑93 (MQ=255)

                                                  aaGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAgg                                        >  2:3975344/1‑93 (MQ=255)

                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                   <  2:7804096/93‑1 (MQ=255)

                                                          agaTTGGAATACAATTTCTCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGAGTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatta                                <  1:5098039/93‑1 (MQ=255)

                                                          agaTTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatta                                >  2:3439040/1‑93 (MQ=255)

                                                           gaTTGGAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                               >  2:3033271/1‑93 (MQ=255)

                                                               ggACTACAATTTCGCGCATTTTG‑TTTTTATGGG‑GCTTGGCCGCCAAACGATTTTTTATATCACGGGGAGACTCTCAGAGGCGTTACTAACCac                           >  2:275666/1‑92 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                          >  1:2485646/1‑93 (MQ=255)

                                                                gAATACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                          >  2:6317653/1‑93 (MQ=255)

                                                                 aaTACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                         <  1:2790856/93‑1 (MQ=255)

                                                                  aTACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt                        >  2:5485673/1‑93 (MQ=255)

                                                                   tACAATTTCGCGCTTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATTACGGGGAGCCTCTCACAGGCGTTATTACCCAACTTg                       >  1:6844881/1‑93 (MQ=255)

                                                                   tACAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTg                       <  2:3677262/93‑1 (MQ=255)

                                                                    aCAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                      <  1:7041877/93‑1 (MQ=255)

                                                                     cAATTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                     <  2:1026320/93‑1 (MQ=255)

                                                                      aaTTTCGCGCCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAgg                    <  2:7667942/93‑1 (MQ=255)

                                                                        gttCGCGCTTTTTGTTTTTTAG‑GG‑CCGTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTTTCAGAGGCGTTATTACCCAACTTGAGGaa                  <  2:2448122/92‑1 (MQ=255)

                                                                          tCGCGCCTTTTG‑TTTTTATGGG‑CCTTCCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAtt                <  2:4318636/93‑1 (MQ=255)

                                                                            gcgcCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta              >  1:67203/1‑93 (MQ=255)

                                                                             tgcattttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGTCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat             <  2:2266834/89‑1 (MQ=255)

                                                                             cgcCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat             >  1:3642188/1‑93 (MQ=255)

                                                                             cgcCTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat             <  1:2228247/93‑1 (MQ=255)

                                                                               ccTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATaa           >  1:4771835/1‑93 (MQ=255)

                                                                                cTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGTGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt          >  1:7935427/1‑93 (MQ=255)

                                                                                cTTTTG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAt          <  2:5582532/93‑1 (MQ=255)

                                                                                 ttttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  2:6574613/1‑93 (MQ=255)

                                                                                 ttttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGACTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg         >  1:8059715/1‑93 (MQ=255)

                                                                                  tttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATgg        >  2:7312355/1‑93 (MQ=255)

                                                                                  tttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATgg        <  1:5617729/93‑1 (MQ=255)

                                                                                   ttG‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGc       <  1:7059912/93‑1 (MQ=255)

                                                                                     g‑TTTTTATGGG‑CCTTGCCCTTAAATCGATTTTTAATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTa     <  2:5280496/93‑1 (MQ=255)

                                                                                     g‑TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTa     >  1:2359397/1‑93 (MQ=255)

                                                                                       tttttATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTaa    >  2:6948336/1‑93 (MQ=255)

                                                                                        ttttATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAg   <  1:4500172/93‑1 (MQ=255)

                                                                                         tttATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGa  <  1:1392514/93‑1 (MQ=255)

                                                                                         tttAT‑GGTCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGa  <  2:8792402/93‑1 (MQ=255)

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG‑TTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA  >  NC_000913/4175855‑4176034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               >  SRR4302541.3047428/1‑94 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302541.2994446/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               <  SRR4302541.1473931/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                               >  SRR4302541.2387505/1‑94 (MQ=60)

 AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                              <  SRR4302541.1136224/94‑1 (MQ=60)

 AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGG                                                                                              <  SRR4302541.8361761/94‑1 (MQ=60)

  AAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGGC                                                                                             >  SRR4302541.3923471/1‑94 (MQ=60)

   AAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgcc                                                                                            <  SRR4302541.6941050/94‑4 (MQ=60)

    AAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCT                                                                                            <  SRR4302541.4141300/94‑1 (MQ=60)

     AGCCTACCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           <  SRR4302541.4400870/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           <  SRR4302541.3107357/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           <  SRR4302541.8452274/94‑1 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           >  SRR4302541.428930/1‑94 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           >  SRR4302541.5643256/1‑94 (MQ=60)

     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTT                                                                                           <  SRR4302541.7423684/94‑1 (MQ=60)

      GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTG                                                                                          <  SRR4302541.5572237/94‑1 (MQ=60)

      GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTG                                                                                          <  SRR4302541.7150610/94‑1 (MQ=60)

       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGC                                                                                         >  SRR4302541.7463789/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCC                                                                                        >  SRR4302541.6521291/1‑94 (MQ=60)

        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCC                                                                                        >  SRR4302541.2314090/1‑94 (MQ=60)

         TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCC                                                                                       <  SRR4302541.568458/94‑1 (MQ=60)

           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGT                                                                                     >  SRR4302541.838425/1‑94 (MQ=60)

           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGT                                                                                     >  SRR4302541.6560309/1‑94 (MQ=60)

           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGT                                                                                     >  SRR4302541.1496575/1‑94 (MQ=60)

           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGT                                                                                     >  SRR4302541.1496578/1‑94 (MQ=60)

             CCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAA                                                                                   >  SRR4302541.8753074/1‑94 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA                                                                                 <  SRR4302541.1696332/94‑1 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA                                                                                 >  SRR4302541.1299888/1‑94 (MQ=60)

               AGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA                                                                                 >  SRR4302541.7700303/1‑94 (MQ=60)

                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAC                                                                                <  SRR4302541.2839779/94‑1 (MQ=60)

                 CGATCAAAAAAGCGTCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACG                                                                               <  SRR4302541.5297310/94‑1 (MQ=60)

                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACG                                                                               >  SRR4302541.5341064/1‑94 (MQ=60)

                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACG                                                                               <  SRR4302541.805480/94‑1 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGT                                                                              >  SRR4302541.7702678/1‑94 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGA                                                                              >  SRR4302541.4518544/1‑94 (MQ=60)

                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTAGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGA                                                                              >  SRR4302541.5008310/1‑94 (MQ=60)

                   ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAT                                                                             >  SRR4302541.3090606/1‑94 (MQ=60)

                    TCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATT                                                                            <  SRR4302541.6814263/94‑1 (MQ=60)

                    TCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATT                                                                            <  SRR4302541.8274066/94‑1 (MQ=60)

                    TCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGAAAAACGATT                                                                            <  SRR4302541.8274304/94‑1 (MQ=60)

                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTT                                                                          >  SRR4302541.8252625/1‑94 (MQ=60)

                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTA                                                                       >  SRR4302541.6485136/1‑94 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTAT                                                                      >  SRR4302541.6534275/1‑94 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302541.8269516/94‑1 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302541.7388005/94‑1 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTAT                                                                      >  SRR4302541.4347054/1‑94 (MQ=60)

                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTAT                                                                      >  SRR4302541.4274544/1‑94 (MQ=60)

                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATA                                                                     >  SRR4302541.5614548/1‑94 (MQ=60)

                           AGCGGCGATTTAATCGTCGCACAAGGCGTGAGATTGGAATCCAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATATTTTATA                                                                     <  SRR4302541.5562661/94‑1 (MQ=60)

                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATAT                                                                    <  SRR4302541.276996/94‑1 (MQ=60)

                             CGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATC                                                                   >  SRR4302541.4449470/1‑94 (MQ=60)

                              GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302541.3982357/94‑1 (MQ=60)

                              GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302541.6264784/94‑1 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 <  SRR4302541.2187163/94‑1 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 >  SRR4302541.7094068/1‑94 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 >  SRR4302541.5921487/1‑94 (MQ=60)

                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 >  SRR4302541.7619156/1‑94 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTTGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACG                                                                >  SRR4302541.1256312/1‑94 (MQ=60)

                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302541.387935/94‑1 (MQ=60)

                                 GATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGG                                                               <  SRR4302541.443992/94‑1 (MQ=60)

                                  ATTTAATCGTTGCACAAGGCGTTAAATTTGAATTCAATTTCGCGCCTTTTTTTTTTATGGGCCTTGCCCCTAAAACGATTTTTTATATCACGGG                                                              <  SRR4302541.4823015/94‑1 (MQ=60)

                                  ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGG                                                              <  SRR4302541.5886891/94‑1 (MQ=60)

                                  ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGG                                                              <  SRR4302541.3853343/94‑1 (MQ=60)

                                   TTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGG                                                             >  SRR4302541.5340139/1‑94 (MQ=60)

                                    TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                            >  SRR4302541.2844665/1‑94 (MQ=60)

                                     TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                           <  SRR4302541.5749934/94‑1 (MQ=60)

                                     TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                           <  SRR4302541.5696347/94‑1 (MQ=60)

                                      AATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGC                                                          >  SRR4302541.6771779/1‑94 (MQ=60)

                                      AATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGC                                                          >  SRR4302541.6771780/1‑94 (MQ=60)

                                       ATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         >  SRR4302541.7974845/1‑94 (MQ=60)

                                       ATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         >  SRR4302541.1198868/1‑94 (MQ=60)

                                        TCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCT                                                        >  SRR4302541.1750152/1‑94 (MQ=60)

                                        TCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCT                                                        <  SRR4302541.2376906/94‑1 (MQ=60)

                                        TCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCT                                                        >  SRR4302541.1116462/1‑94 (MQ=60)

                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302541.5722594/1‑94 (MQ=60)

                                          GTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCT                                                      <  SRR4302541.7369947/94‑1 (MQ=60)

                                           TTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTC                                                     <  SRR4302541.6606218/94‑1 (MQ=60)

                                             GCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAG                                                   <  SRR4302541.5835427/94‑1 (MQ=60)

                                               ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 <  SRR4302541.652328/94‑1 (MQ=60)

                                               ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 <  SRR4302541.6096943/94‑1 (MQ=60)

                                                CAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCTCGTAAAACGATTTTTTATATCACGGGAAGACTCTCAGAGG                                                >  SRR4302541.2615932/1‑94 (MQ=60)

                                                CAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGG                                                >  SRR4302541.140602/1‑94 (MQ=60)

                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               >  SRR4302541.6429052/1‑94 (MQ=60)

                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               >  SRR4302541.2457924/1‑94 (MQ=60)

                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                           >  SRR4302541.6590388/1‑94 (MQ=60)

                                                       TGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATT                                         <  SRR4302541.4012438/94‑1 (MQ=60)

                                                       TGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATT                                         >  SRR4302541.4650896/1‑94 (MQ=60)

                                                       TGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATT                                         >  SRR4302541.2012632/1‑94 (MQ=60)

                                                         AGATTGGAATACAATTTCTCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAGTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       <  SRR4302541.5150419/94‑1 (MQ=60)

                                                         AGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       <  SRR4302541.6857159/94‑1 (MQ=60)

                                                            TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    <  SRR4302541.3837650/94‑1 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302541.2510639/1‑94 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  <  SRR4302541.3575033/94‑1 (MQ=60)

                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302541.2510638/1‑94 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                <  SRR4302541.665914/94‑1 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                >  SRR4302541.1451806/1‑94 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                <  SRR4302541.2819037/94‑1 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                <  SRR4302541.7703511/94‑1 (MQ=60)

                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                >  SRR4302541.8056052/1‑94 (MQ=60)

                                                                 ATACAATTTCGCGCTTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATTACGGGGAGCCTCTCACAGGCGTTATTACCCAACTTG                               >  SRR4302541.6918068/1‑94 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302541.7116752/94‑1 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302541.7122691/94‑1 (MQ=60)

                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302541.5770889/94‑1 (MQ=60)

                                                                     AATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA                           >  SRR4302541.5507653/1‑94 (MQ=60)

                                                                     AATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA                           >  SRR4302541.6673186/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      >  SRR4302541.67758/1‑94 (MQ=60)

                                                                          CGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      <  SRR4302541.2227460/94‑1 (MQ=60)

                                                                           GCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTAT                     <  SRR4302541.3969748/94‑1 (MQ=60)

                                                                           GCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTAT                     >  SRR4302541.3679126/1‑94 (MQ=60)

                                                                            CGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    <  SRR4302541.2249830/94‑1 (MQ=60)

                                                                             GCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGCATTTATAA                   >  SRR4302541.625/1‑94 (MQ=60)

                                                                             GCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAA                   >  SRR4302541.4527233/1‑94 (MQ=60)

                                                                             GCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAA                   >  SRR4302541.4820558/1‑94 (MQ=60)

                                                                              CCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGTGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  >  SRR4302541.8022822/1‑94 (MQ=60)

                                                                              CCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  <  SRR4302541.1868526/94‑1 (MQ=60)

                                                                               CTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGACTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 >  SRR4302541.8148270/1‑94 (MQ=60)

                                                                                 TTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGC               <  SRR4302541.5675220/94‑1 (MQ=60)

                                                                                 TTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATAGC               >  SRR4302541.5164649/1‑94 (MQ=60)

                                                                                  TTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT              <  SRR4302541.7134996/94‑1 (MQ=60)

                                                                                  TTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCT              <  SRR4302541.1519554/94‑1 (MQ=60)

                                                                                   TGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTA             >  SRR4302541.2382380/1‑94 (MQ=60)

                                                                                     TTTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACCTGAGGAATTTATAATGGCTAAG           <  SRR4302541.280355/94‑1 (MQ=60)

                                                                                      TTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA          >  SRR4302541.3934829/1‑94 (MQ=60)

                                                                                      TTTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGA          <  SRR4302541.4545565/94‑1 (MQ=60)

                                                                                       TTTATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA         <  SRR4302541.1406162/94‑1 (MQ=60)

                                                                                         TATGGGCCTTGCTCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       <  SRR4302541.1296736/94‑1 (MQ=60)

                                                                                         TATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       <  SRR4302541.8904873/94‑1 (MQ=60)

                                                                                         TATGGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAG       >  SRR4302541.3241961/1‑94 (MQ=60)

                                                                                          atgGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGT      >  SRR4302541.8315392/4‑94 (MQ=60)

                                                                                           tgGGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGTGGAATTTATAATGGCTAAGAAAGTA     >  SRR4302541.5199759/3‑94 (MQ=60)

                                                                                           G‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    <  SRR4302541.5086431/94‑1 (MQ=60)

                                                                                           G‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    >  SRR4302541.4095003/1‑94 (MQ=60)

                                                                                           G‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    >  SRR4302541.311929/1‑94 (MQ=60)

                                                                                             GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302541.617914/94‑1 (MQ=60)

                                                                                             GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   <  SRR4302541.5038161/94‑1 (MQ=60)

                                                                                              GCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  <  SRR4302541.6982463/94‑1 (MQ=60)

                                                                                              GCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  <  SRR4302541.5224417/94‑1 (MQ=60)

GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`