Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,169 A→G H1116R (CAC→CGC)  rpoB → RNA polymerase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,1690AG99.3% 994.3 / NA 292H1116R (CAC→CGC) rpoBRNA polymerase beta subunit
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (146/144);  total (147/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258
                                                                                          |                                                                                         
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         <  1:4125951/93‑1 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         <  1:7199222/93‑1 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         <  2:14986/93‑1 (MQ=255)
cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         >  2:5483063/1‑93 (MQ=255)
  tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTg                                                                                       <  1:8634119/93‑1 (MQ=255)
   aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTgg                                                                                      <  2:8148671/93‑1 (MQ=255)
     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGt                                                                                    >  2:7629209/1‑93 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACGTCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTa                                                                                   <  2:4746079/93‑1 (MQ=255)
      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTa                                                                                   >  1:4921710/1‑93 (MQ=255)
         aaaaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATgg                                                                                >  2:8414368/1‑93 (MQ=255)
         aaaaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGGATgg                                                                                >  1:7324157/1‑93 (MQ=255)
          aaaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGc                                                                               >  1:6903683/1‑93 (MQ=255)
          aaaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGc                                                                               >  1:810447/1‑93 (MQ=255)
           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCt                                                                              >  2:7784319/1‑93 (MQ=255)
             cGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGc                                                                            <  1:6064912/93‑1 (MQ=255)
             cGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGc                                                                            >  1:1033833/1‑93 (MQ=255)
              cgTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCg                                                                           <  2:6492517/92‑1 (MQ=255)
                tACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGaa                                                                         >  2:7725523/1‑93 (MQ=255)
                  cGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAGCCCGCCTGGGTATGGCTGCGAAAg                                                                       <  2:3769252/93‑1 (MQ=255)
                   gCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAgg                                                                      >  2:1086364/1‑93 (MQ=255)
                     cGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTa                                                                    <  2:6705225/93‑1 (MQ=255)
                      ggTATACATCGTACTGAGCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTAt                                                                   <  1:3521209/93‑1 (MQ=255)
                        tAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCg                                                                 >  1:7123002/1‑93 (MQ=255)
                        tAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCg                                                                 <  1:3130678/93‑1 (MQ=255)
                        tAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCg                                                                 >  1:3040641/1‑93 (MQ=255)
                           aCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              >  2:2068552/1‑93 (MQ=255)
                           aCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              <  2:8035922/93‑1 (MQ=255)
                           aCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              >  1:1827343/1‑93 (MQ=255)
                              tCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATTGGGGACa                                                           >  2:7540048/1‑93 (MQ=255)
                               cGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          <  1:6480362/93‑1 (MQ=255)
                               cGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACaa                                                          <  1:5487386/93‑1 (MQ=255)
                                 tACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGa                                                        >  2:4969404/1‑93 (MQ=255)
                                 tACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                        <  1:4164538/93‑1 (MQ=255)
                                  aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  1:3661805/93‑1 (MQ=255)
                                  aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGAt                                                       >  1:4412123/1‑93 (MQ=255)
                                   cTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                      >  1:5267300/1‑93 (MQ=255)
                                   cTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATc                                                      <  1:4839944/93‑1 (MQ=255)
                                   cTGAACCCGCTGGGCGTACCGTCTCGGATGAACATCGGTCAGATCCTTGAAACCCGCCTGGGCATGGCTGCGAAAGGTATCGGCGACAAGATc                                                      >  2:8610989/1‑93 (MQ=255)
                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                    >  2:7129050/1‑93 (MQ=255)
                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                    >  1:5533519/1‑93 (MQ=255)
                                      aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   <  2:4250923/93‑1 (MQ=255)
                                      aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   >  1:7947047/1‑93 (MQ=255)
                                          cGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  2:4059813/93‑1 (MQ=255)
                                           gCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                              <  1:6762329/93‑1 (MQ=255)
                                             tGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                            >  2:513358/1‑93 (MQ=255)
                                              gggCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                           <  2:6237241/93‑1 (MQ=255)
                                              gggCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                           >  1:1953853/1‑93 (MQ=255)
                                              gggCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                           >  2:3231203/1‑93 (MQ=255)
                                                 cGTACCGTCTCGTATGAACATCAGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGATCAACGCCATGCTGaa                                        >  2:5526121/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  2:7010675/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  2:8308976/1‑93 (MQ=255)
                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  2:3663504/1‑93 (MQ=255)
                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                      >  1:6332489/1‑93 (MQ=255)
                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                      <  2:5783134/93‑1 (MQ=255)
                                                    gccGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     <  2:7258179/92‑1 (MQ=255)
                                                    aCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     <  1:3351215/93‑1 (MQ=255)
                                                      cGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                   <  2:243497/93‑1 (MQ=255)
                                                       gTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGTAAGGTATCGGCGGCAAGATCAACGACATGCTGAAAcagca                                  >  2:4661515/1‑93 (MQ=255)
                                                       gTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                  <  1:6380414/93‑1 (MQ=255)
                                                        tctcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                 >  1:8763890/1‑93 (MQ=255)
                                                        tctcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                 <  1:8505052/93‑1 (MQ=255)
                                                         ctcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCCACGCCATGCTGAAACaacagc                                >  2:3489779/1‑93 (MQ=255)
                                                           cGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                              <  1:7651191/93‑1 (MQ=255)
                                                            gTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                             <  1:4547484/93‑1 (MQ=255)
                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:7893987/93‑1 (MQ=255)
                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:60543/93‑1 (MQ=255)
                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGATATGGTTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  2:6870082/93‑1 (MQ=255)
                                                               tGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                          <  2:3422596/93‑1 (MQ=255)
                                                               tGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                          <  1:7760531/93‑1 (MQ=255)
                                                                gAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt                         <  1:2254415/93‑1 (MQ=255)
                                                                gAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGt                         >  2:5282887/1‑93 (MQ=255)
                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  2:2660505/93‑1 (MQ=255)
                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        >  1:1189976/1‑93 (MQ=255)
                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  1:3875859/93‑1 (MQ=255)
                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  1:4274132/93‑1 (MQ=255)
                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        >  2:4882213/1‑93 (MQ=255)
                                                                   cATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      >  1:2939628/1‑93 (MQ=255)
                                                                    aTCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                     >  1:1678986/1‑93 (MQ=255)
                                                                        gTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 >  1:2176983/1‑93 (MQ=255)
                                                                         tCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt                <  2:1992112/93‑1 (MQ=255)
                                                                          cAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg               <  2:8578709/93‑1 (MQ=255)
                                                                          cAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg               <  2:2281377/93‑1 (MQ=255)
                                                                          cAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg               >  1:2598484/1‑93 (MQ=255)
                                                                           aGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc              <  2:3897407/93‑1 (MQ=255)
                                                                           aGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgc              <  1:2570815/93‑1 (MQ=255)
                                                                            gATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAAAAGTCGCGAAACTgcg             >  2:4426865/1‑93 (MQ=255)
                                                                              tCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg           >  1:1560842/1‑93 (MQ=255)
                                                                                 tCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        >  1:7338806/1‑93 (MQ=255)
                                                                                 tCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        >  1:5773238/1‑93 (MQ=255)
                                                                                 tCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt        <  2:6014804/93‑1 (MQ=255)
                                                                                  cGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt       <  2:3608134/93‑1 (MQ=255)
                                                                                  cGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt       >  1:8129855/1‑93 (MQ=255)
                                                                                    aaaCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa     >  1:6453098/1‑93 (MQ=255)
                                                                                     aaCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt    >  2:1625610/1‑93 (MQ=255)
                                                                                      gcccGCCTGGGGAGGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   <  1:6222292/92‑1 (MQ=255)
                                                                                      aCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   >  2:7427710/1‑93 (MQ=255)
                                                                                      aCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc   <  1:6969254/93‑1 (MQ=255)
                                                                                       cccGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  <  1:5115927/93‑1 (MQ=255)
                                                                                       cccGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  <  2:3021863/93‑1 (MQ=255)
                                                                                       cccGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  <  1:1435261/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4182076‑4182261
                                                                                             |                                                                                            
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCG                                                                                              >  SRR4302541.4586122/1‑94 (MQ=60)
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCG                                                                                              <  SRR4302541.4692482/94‑1 (MQ=60)
 GCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGC                                                                                             >  SRR4302541.7103770/1‑94 (MQ=60)
 GCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGC                                                                                             >  SRR4302541.8542111/1‑94 (MQ=60)
  CCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCC                                                                                            <  SRR4302541.7294178/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.8193350/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           >  SRR4302541.6035137/1‑94 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.7276025/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.7276024/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.4166707/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.6857012/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.4166705/94‑1 (MQ=60)
   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           >  SRR4302541.1805000/1‑94 (MQ=60)
   CTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                           <  SRR4302541.6857013/94‑1 (MQ=60)
     TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGG                                                                                         <  SRR4302541.8726627/94‑1 (MQ=60)
     TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGG                                                                                         >  SRR4302541.740478/1‑94 (MQ=60)
     TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATACTCGAAACCCGTCTGG                                                                                         >  SRR4302541.6213336/1‑94 (MQ=60)
      ACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGG                                                                                        <  SRR4302541.5778908/94‑1 (MQ=60)
       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGT                                                                                       >  SRR4302541.2257596/1‑94 (MQ=60)
        GATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTA                                                                                      >  SRR4302541.4971926/1‑94 (MQ=60)
         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTAT                                                                                     <  SRR4302541.5588498/94‑1 (MQ=60)
         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTAT                                                                                     >  SRR4302541.2901333/1‑94 (MQ=60)
          TGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTTAGATCCTCGAAACCCGCCTGGGTATG                                                                                    <  SRR4302541.1475453/94‑1 (MQ=60)
           GAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGG                                                                                   <  SRR4302541.4967392/94‑1 (MQ=60)
           GAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGG                                                                                   >  SRR4302541.8288808/1‑94 (MQ=60)
           GAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGGATGG                                                                                   >  SRR4302541.7402369/1‑94 (MQ=60)
            AAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGC                                                                                  >  SRR4302541.6977350/1‑94 (MQ=60)
            AAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGC                                                                                  >  SRR4302541.6977349/1‑94 (MQ=60)
            AAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGC                                                                                  >  SRR4302541.2775372/1‑94 (MQ=60)
            AAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGC                                                                                  >  SRR4302541.817866/1‑94 (MQ=60)
             AAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCT                                                                                 >  SRR4302541.8607011/1‑94 (MQ=60)
             AAACGGTACGCCGGTAGACATAGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAACCTCGAAACCCGCCTGGGTATGGCT                                                                                 >  SRR4302541.746520/1‑94 (MQ=60)
              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTG                                                                                <  SRR4302541.3067634/94‑1 (MQ=60)
              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTG                                                                                >  SRR4302541.4048428/1‑94 (MQ=60)
               ACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGC                                                                               >  SRR4302541.1043377/1‑94 (MQ=60)
                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCG                                                                              <  SRR4302541.6126850/94‑1 (MQ=60)
                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGA                                                                             <  SRR4302541.4179670/94‑1 (MQ=60)
                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGA                                                                             <  SRR4302541.4179668/94‑1 (MQ=60)
                      GCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGT                                                                        <  SRR4302541.1873280/94‑1 (MQ=60)
                      GCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGT                                                                        <  SRR4302541.4883852/94‑1 (MQ=60)
                       CCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTA                                                                       >  SRR4302541.2498541/1‑94 (MQ=60)
                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTAT                                                                      >  SRR4302541.6364019/1‑94 (MQ=60)
                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGAT                                                                      >  SRR4302541.6479433/1‑94 (MQ=60)
                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGCAT                                                                      >  SRR4302541.3432375/1‑94 (MQ=60)
                         GGTATACATCGTACTGAGCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302541.3556636/94‑1 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     >  SRR4302541.5347127/1‑94 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302541.3556635/94‑1 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302541.8009647/94‑1 (MQ=60)
                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                     <  SRR4302541.7736206/94‑1 (MQ=60)
                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                    >  SRR4302541.3071369/1‑94 (MQ=60)
                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                    >  SRR4302541.7198877/1‑94 (MQ=60)
                           gcacacttcgtattaacccgctgggcggagcggcggcTATGAACATCGGTCAGATCCTGGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGG                                                                   <  SRR4302541.7302614/57‑1 (MQ=60)
                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGG                                                                   <  SRR4302541.7302613/94‑1 (MQ=60)
                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGG                                                                   <  SRR4302541.3162320/94‑1 (MQ=60)
                            AGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGC                                                                  >  SRR4302541.3896367/1‑94 (MQ=60)
                            AGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCATCGAAACCCGCCTGGGTATGGCGGCGAAAGGTttcgcg                                                                  >  SRR4302541.1874968/1‑88 (MQ=60)
                             GACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCG                                                                 >  SRR4302541.1845066/1‑94 (MQ=60)
                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGA                                                                >  SRR4302541.6570561/1‑94 (MQ=60)
                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGA                                                                >  SRR4302541.6470807/1‑94 (MQ=60)
                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCCAAAGGTATCGGCGA                                                                >  SRR4302541.1683880/1‑94 (MQ=60)
                               CATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAC                                                               >  SRR4302541.1327752/1‑94 (MQ=60)
                               CATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAC                                                               >  SRR4302541.7402057/1‑94 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                              >  SRR4302541.4904002/1‑94 (MQ=60)
                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                              <  SRR4302541.1076077/94‑1 (MQ=60)
                                  CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAG                                                            <  SRR4302541.5543629/94‑1 (MQ=60)
                                  CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAG                                                            <  SRR4302541.8001518/94‑1 (MQ=60)
                                  CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAG                                                            <  SRR4302541.6546410/94‑1 (MQ=60)
                                  CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAG                                                            >  SRR4302541.2120215/1‑94 (MQ=60)
                                    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAT                                                          <  SRR4302541.4205778/94‑1 (MQ=60)
                                    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGAT                                                          >  SRR4302541.4456330/1‑94 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         >  SRR4302541.5321570/1‑94 (MQ=60)
                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                         <  SRR4302541.3698956/94‑1 (MQ=60)
                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                        <  SRR4302541.4889277/94‑1 (MQ=60)
                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                        >  SRR4302541.4828889/1‑94 (MQ=60)
                                       TGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAA                                                       >  SRR4302541.5590164/1‑94 (MQ=60)
                                        GAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAC                                                      >  SRR4302541.8034591/1‑94 (MQ=60)
                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCGGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAATATCAAgc                                                     >  SRR4302541.3901129/1‑92 (MQ=60)
                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAGAAGATCAACA                                                     >  SRR4302541.8165229/1‑94 (MQ=60)
                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                     >  SRR4302541.3166931/1‑94 (MQ=60)
                                          ACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGATCAACGC                                                    >  SRR4302541.6693479/1‑94 (MQ=60)
                                           CCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCC                                                   >  SRR4302541.5647339/1‑94 (MQ=60)
                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                  >  SRR4302541.8275092/1‑94 (MQ=60)
                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGCGAAGGCCGGCTGGGGGGGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                  <  SRR4302541.8004398/94‑1 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 <  SRR4302541.600043/94‑1 (MQ=60)
                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                 <  SRR4302541.4419047/94‑1 (MQ=60)
                                              GCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATG                                                <  SRR4302541.6834645/94‑1 (MQ=60)
                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                              >  SRR4302541.1972685/1‑94 (MQ=60)
                                                 GGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTG                                             <  SRR4302541.7098450/94‑1 (MQ=60)
                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                         <  SRR4302541.6462121/94‑1 (MQ=60)
                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                         >  SRR4302541.6397231/1‑94 (MQ=60)
                                                      TACCGTCTGGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACA                                        >  SRR4302541.4276224/1‑94 (MQ=60)
                                                       GCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302541.534191/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       >  SRR4302541.7599283/1‑94 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302541.3384687/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302541.2270719/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302541.4649967/94‑1 (MQ=60)
                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                       <  SRR4302541.3172955/94‑1 (MQ=60)
                                                         CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGGAAGGTATCGGCGACAAGATCAAAGCCATGCTGAAACAGAA                                     >  SRR4302541.1135761/1‑94 (MQ=60)
                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    <  SRR4302541.6445591/94‑1 (MQ=60)
                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    >  SRR4302541.8857616/1‑94 (MQ=60)
                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    <  SRR4302541.514955/94‑1 (MQ=60)
                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    <  SRR4302541.8216788/94‑1 (MQ=60)
                                                          CTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                    <  SRR4302541.2491721/94‑1 (MQ=60)
                                                           TCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGC                                   <  SRR4302541.8596631/94‑1 (MQ=60)
                                                            CTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCA                                  <  SRR4302541.1779659/94‑1 (MQ=60)
                                                            CTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCA                                  >  SRR4302541.3612119/1‑94 (MQ=60)
                                                              TGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                >  SRR4302541.5556966/1‑94 (MQ=60)
                                                              CGTATGACCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                <  SRR4302541.6327668/94‑1 (MQ=60)
                                                              CGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                <  SRR4302541.3085076/94‑1 (MQ=60)
                                                              CGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                <  SRR4302541.315070/94‑1 (MQ=60)
                                                              CGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAG                                <  SRR4302541.7732702/94‑1 (MQ=60)
                                                               GTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                               <  SRR4302541.4593483/94‑1 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             >  SRR4302541.4550030/1‑94 (MQ=60)
                                                                 ATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                             <  SRR4302541.8762059/94‑1 (MQ=60)
                                                                  TGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGGTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGT                            <  SRR4302541.8127813/94‑1 (MQ=60)
                                                                  TGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGT                            >  SRR4302541.2082714/1‑94 (MQ=60)
                                                                  TGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGT                            <  SRR4302541.7843100/94‑1 (MQ=60)
                                                                   GAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                           >  SRR4302541.1201492/1‑94 (MQ=60)
                                                                   GAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                           >  SRR4302541.6175407/1‑94 (MQ=60)
                                                                   GAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                           <  SRR4302541.2276188/94‑1 (MQ=60)
                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                          <  SRR4302541.4316828/94‑1 (MQ=60)
                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                          >  SRR4302541.6404630/1‑94 (MQ=60)
                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                          <  SRR4302541.3005115/94‑1 (MQ=60)
                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                          <  SRR4302541.3914805/94‑1 (MQ=60)
                                                                     ACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGC                         >  SRR4302541.2969193/1‑94 (MQ=60)
                                                                     ACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGC                         >  SRR4302541.5173201/1‑94 (MQ=60)
                                                                      CATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                        <  SRR4302541.7836149/94‑1 (MQ=60)
                                                                      CATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                        >  SRR4302541.1695508/1‑94 (MQ=60)
                                                                      CATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                        <  SRR4302541.4223985/94‑1 (MQ=60)
                                                                        TCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAA                      >  SRR4302541.4578758/1‑94 (MQ=60)
                                                                         CGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAA                     <  SRR4302541.2610621/94‑1 (MQ=60)
                                                                         CGGTCAGATCCGCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAA                     <  SRR4302541.8624821/94‑1 (MQ=60)
                                                                          GGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAC                    >  SRR4302541.2198017/1‑94 (MQ=60)
                                                                           GTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                   <  SRR4302541.979257/94‑1 (MQ=60)
                                                                           GTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                   <  SRR4302541.7055968/94‑1 (MQ=60)
                                                                           GTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                   <  SRR4302541.1306305/94‑1 (MQ=60)
                                                                           GTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                   <  SRR4302541.6543626/94‑1 (MQ=60)
                                                                            TCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTG                  >  SRR4302541.2624509/1‑94 (MQ=60)
                                                                             CAGATCCTCGAAGCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGC                 <  SRR4302541.438314/94‑1 (MQ=60)
                                                                             CAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGC                 <  SRR4302541.6544179/94‑1 (MQ=60)
                                                                              AGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCG                <  SRR4302541.2596590/94‑1 (MQ=60)
                                                                               GATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGC               <  SRR4302541.6418453/94‑1 (MQ=60)
                                                                                ATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG              >  SRR4302541.7208208/1‑94 (MQ=60)
                                                                                ATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG              >  SRR4302541.1576467/1‑94 (MQ=60)
                                                                                 TCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGA             >  SRR4302541.3596200/1‑94 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           <  SRR4302541.1497920/94‑1 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           >  SRR4302541.5831959/1‑94 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           >  SRR4302541.7417177/1‑94 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           <  SRR4302541.2998485/94‑1 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           >  SRR4302541.7417176/1‑94 (MQ=60)
                                                                                   CTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGACATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAT           >  SRR4302541.5226254/1‑94 (MQ=60)
                                                                                    TCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT          >  SRR4302541.8218851/1‑94 (MQ=60)
                                                                                     CGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC         >  SRR4302541.1652570/1‑94 (MQ=60)
                                                                                      GAAACCCGGCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA        <  SRR4302541.8728952/94‑1 (MQ=60)
                                                                                      GAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA        >  SRR4302541.6518863/1‑94 (MQ=60)
                                                                                       AAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT       >  SRR4302541.6992270/1‑94 (MQ=60)
                                                                                        AACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC      <  SRR4302541.3828357/94‑1 (MQ=60)
                                                                                        AACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC      >  SRR4302541.2612807/1‑94 (MQ=60)
                                                                                         ACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC     <  SRR4302541.7043529/94‑1 (MQ=60)
                                                                                         ACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC     >  SRR4302541.5132496/1‑94 (MQ=60)
                                                                                          CCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA    >  SRR4302541.2597567/1‑94 (MQ=60)
                                                                                          CCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA    <  SRR4302541.1449217/94‑1 (MQ=60)
                                                                                          CCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA    <  SRR4302541.5168478/94‑1 (MQ=60)
                                                                                           CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG   <  SRR4302541.8544968/94‑1 (MQ=60)
                                                                                            CGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  <  SRR4302541.2453247/94‑1 (MQ=60)
                                                                                            CGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  <  SRR4302541.4152173/94‑1 (MQ=60)
                                                                                             |                                                                                            
TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4182076‑4182261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑