Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,169	A→G	H1116R (CAC→CGC)	rpoB →	RNA polymerase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,169	0	A	G	99.3%	967.7 / ‑4.8	284	H1116R (CAC→CGC)	rpoB	RNA polymerase beta subunit
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (156/126); total (158/126)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 292 total aligned reads displayed.

CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258

cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         >  2:4277757/1‑93 (MQ=255)

cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         <  1:1416603/93‑1 (MQ=255)

cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         <  1:4831201/93‑1 (MQ=255)

cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGcc                                                                                         >  2:2389968/1‑93 (MQ=255)

 ttACGATGAAAGCGGTACGCCGGTAGCCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGTTCAGATCCTCGAAACCCGCCt                                                                                        <  1:97383/93‑1 (MQ=255)

 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCt                                                                                        >  2:1539453/1‑93 (MQ=255)

 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCt                                                                                        >  1:2671637/1‑93 (MQ=255)

 ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCt                                                                                        >  1:2382686/1‑93 (MQ=255)

   aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTgg                                                                                      <  2:6030579/93‑1 (MQ=255)

     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGt                                                                                    >  1:6112874/1‑93 (MQ=255)

      aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCCCGGATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTa                                                                                   >  2:4145315/1‑93 (MQ=255)

       tGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTAt                                                                                  >  2:3839162/1‑93 (MQ=255)

           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCt                                                                              >  2:5839642/1‑93 (MQ=255)

           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCt                                                                              >  2:1801868/1‑93 (MQ=255)

           aaCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCt                                                                              >  2:4669776/1‑93 (MQ=255)

            aCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTg                                                                             >  2:527839/1‑93 (MQ=255)

            aCGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACCGCCTGGGTATGGCTg                                                                             >  1:3336143/1‑93 (MQ=255)

              ggTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCg                                                                           >  1:757913/1‑93 (MQ=255)

              ggTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCg                                                                           >  1:2468667/1‑93 (MQ=255)

                tACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGaa                                                                         >  1:404203/1‑93 (MQ=255)

                 aCGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGaaa                                                                        <  2:1737905/93‑1 (MQ=255)

                   gCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGGAAgg                                                                      >  1:393665/1‑93 (MQ=255)

                   gCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAgg                                                                      >  1:881926/1‑93 (MQ=255)

                   gCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCAGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAgg                                                                      <  2:3009418/93‑1 (MQ=255)

                    ccGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGt                                                                     >  2:6435473/1‑93 (MQ=255)

                    ccGGCAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGt                                                                     >  2:4915965/1‑93 (MQ=255)

                     cGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTa                                                                    <  2:4457483/93‑1 (MQ=255)

                      ggTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTAt                                                                   <  1:560175/93‑1 (MQ=255)

                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  >  2:4773649/1‑93 (MQ=255)

                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  <  1:2779818/93‑1 (MQ=255)

                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  >  1:6418336/1‑93 (MQ=255)

                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  >  2:3258082/1‑93 (MQ=255)

                       gTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATc                                                                  <  1:5045665/93‑1 (MQ=255)

                        tAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCg                                                                 <  2:164470/93‑1 (MQ=255)

                         aGACATCGTCCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCgg                                                                <  1:5517492/93‑1 (MQ=255)

                         aGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAACAGTATCgg                                                                >  1:5427044/1‑93 (MQ=255)

                          gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCggg                                                               >  2:5504577/1‑92 (MQ=255)

                          gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGc                                                               <  1:5877764/93‑1 (MQ=255)

                          gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGc                                                               >  2:5399028/1‑93 (MQ=255)

                          gACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGc                                                               >  1:667253/1‑93 (MQ=255)

                           aCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCg                                                              <  2:855161/93‑1 (MQ=255)

                             aTCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                            <  1:3992246/93‑1 (MQ=255)

                                gTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                         <  1:2572203/93‑1 (MQ=255)

                                 tACTGATCCCGCTGGGCGTTCGGTCTCGTATGAACATCGGTCAGATCTTCGAAGCCCGCCTGGGTATGGCTGCGAAGGGTAGCGGCGACAAGa                                                        <  1:2135560/93‑1 (MQ=255)

                                  aCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                       <  1:3538582/93‑1 (MQ=255)

                                    tGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                     <  1:5707210/93‑1 (MQ=255)

                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACCAGATCaa                                                    >  2:5898278/1‑93 (MQ=255)

                                     gAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCaa                                                    <  2:3654380/93‑1 (MQ=255)

                                      aaCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                   <  2:111906/93‑1 (MQ=255)

                                       aCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAACAACg                                                  >  1:4432184/1‑93 (MQ=255)

                                          cGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  2:3516194/93‑1 (MQ=255)

                                          cGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCa                                               <  1:3223520/93‑1 (MQ=255)

                                              gggCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCt                                           >  1:6390319/1‑93 (MQ=255)

                                               ggCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTg                                          >  1:4651556/1‑93 (MQ=255)

                                                  gTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                       >  2:5608437/1‑93 (MQ=255)

                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCGTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaaa                                      >  1:6273820/1‑92 (MQ=255)

                                                   tACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGacac                                      >  1:1033255/1‑93 (MQ=255)

                                                    aCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     >  1:3964687/1‑93 (MQ=255)

                                                    aCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                     <  1:2982441/93‑1 (MQ=255)

                                                      cGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                   >  2:2445603/1‑93 (MQ=255)

                                                         ctcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                <  1:1001704/93‑1 (MQ=255)

                                                          tcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCCACGCCATGCTGAAACAgcagca                               >  1:2606265/1‑93 (MQ=255)

                                                          tcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                               <  1:194124/93‑1 (MQ=255)

                                                          tcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                               <  2:6401132/93‑1 (MQ=255)

                                                          tcGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTCAAACAcgagca                               >  2:4496541/1‑93 (MQ=255)

                                                           cGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                              >  1:1669880/1‑93 (MQ=255)

                                                            gTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                             >  2:813462/1‑93 (MQ=255)

                                                            gTATGAACATCGGTCAGAGCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                             <  1:4313845/93‑1 (MQ=255)

                                                             tATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            >  1:1157242/1‑93 (MQ=255)

                                                             tATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            <  2:4974747/93‑1 (MQ=255)

                                                             tATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                            <  1:2829621/93‑1 (MQ=255)

                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGTTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           >  2:2668168/1‑93 (MQ=255)

                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  1:1506114/93‑1 (MQ=255)

                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           >  2:2227377/1‑93 (MQ=255)

                                                              aTGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaa                           <  1:4634825/93‑1 (MQ=255)

                                                                 aaCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATGGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                        <  2:3434242/93‑1 (MQ=255)

                                                                  aCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       >  1:2269360/1‑93 (MQ=255)

                                                                  aCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       <  2:140228/93‑1 (MQ=255)

                                                                  aCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       >  2:134684/1‑93 (MQ=255)

                                                                  aCATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                       <  1:915403/93‑1 (MQ=255)

                                                                   cATCGGTCAGATCCTCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      <  2:377745/93‑1 (MQ=255)

                                                                   cATCGGTCAGATCCTCGAAACGCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      >  1:4092085/1‑93 (MQ=255)

                                                                   cATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgc                      >  1:1744904/1‑93 (MQ=255)

                                                                    aTCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcgcg                     >  2:6478336/1‑93 (MQ=255)

                                                                       ggTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                  <  1:3153178/93‑1 (MQ=255)

                                                                       ggTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGaaa                  >  1:3740441/1‑93 (MQ=255)

                                                                        gTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 <  1:4522487/93‑1 (MQ=255)

                                                                        gTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                 <  2:3163010/93‑1 (MQ=255)

                                                                         tCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt                <  1:3666466/93‑1 (MQ=255)

                                                                          cAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg               >  2:1383922/1‑93 (MQ=255)

                                                                            gATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg             >  2:1924008/1‑93 (MQ=255)

                                                                            gATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg             >  2:1820732/1‑93 (MQ=255)

                                                                             aTCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc            <  1:5204994/93‑1 (MQ=255)

                                                                               ccTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa          >  2:1158402/1‑93 (MQ=255)

                                                                                  cGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt       >  1:1257045/1‑93 (MQ=255)

                                                                                  cGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAtt       >  2:1268552/1‑93 (MQ=255)

                                                                                    aaaCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa     >  2:5164885/1‑93 (MQ=255)

                                                                                     aaCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAt    >  2:288001/1‑93 (MQ=255)

                                                                                      aCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGTAGCAAGAAGTCGCGAAAATGTGCGAATTCATc   >  2:2783132/1‑93 (MQ=255)

                                                                                       cccGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc  >  2:1059170/1‑93 (MQ=255)

CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC  >  NC_000913/4182079‑4182258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4182076‑4182260

TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCG                                                                                             >  SRR4302534.5623906/1‑94 (MQ=60)

 GCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGCCAGATCCTCTAAACCCGC                                                                                            >  SRR4302534.2568756/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.1343395/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.2445998/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.2742246/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          <  SRR4302534.1453869/94‑1 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.408184/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          <  SRR4302534.4962274/94‑1 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.6577525/1‑94 (MQ=60)

   CTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCT                                                                                          >  SRR4302534.6647308/1‑94 (MQ=60)

    TTACGATGAAAGCGGTACGCCGGTAGCCATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGTTCAGATCCTCGAAACCCGCCTG                                                                                         <  SRR4302534.99987/94‑1 (MQ=60)

    TTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTG                                                                                         <  SRR4302534.3784947/94‑1 (MQ=60)

      ACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGG                                                                                       <  SRR4302534.2769274/94‑1 (MQ=60)

       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGT                                                                                      >  SRR4302534.6279167/1‑94 (MQ=60)

       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGT                                                                                      <  SRR4302534.3588649/94‑1 (MQ=60)

       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGT                                                                                      <  SRR4302534.3345749/94‑1 (MQ=60)

       CGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGT                                                                                      >  SRR4302534.246952/1‑94 (MQ=60)

        GATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTA                                                                                     >  SRR4302534.4717371/1‑94 (MQ=60)

         ATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTAT                                                                                    <  SRR4302534.1033352/94‑1 (MQ=60)

            AAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGC                                                                                 >  SRR4302534.815432/1‑94 (MQ=60)

             AAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCT                                                                                >  SRR4302534.960158/1‑94 (MQ=60)

             AAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCT                                                                                <  SRR4302534.3376860/94‑1 (MQ=60)

             AAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCT                                                                                >  SRR4302534.5643600/1‑94 (MQ=60)

              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTG                                                                               >  SRR4302534.1483216/1‑94 (MQ=60)

              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTG                                                                               <  SRR4302534.2588267/94‑1 (MQ=60)

              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTG                                                                               >  SRR4302534.363312/1‑94 (MQ=60)

              AACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAACCGCCTGGGTATGGCTG                                                                               >  SRR4302534.3425400/1‑94 (MQ=60)

                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCG                                                                             >  SRR4302534.2534286/1‑94 (MQ=60)

                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCG                                                                             >  SRR4302534.6054035/1‑94 (MQ=60)

                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCG                                                                             <  SRR4302534.2316391/94‑1 (MQ=60)

                CGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCG                                                                             >  SRR4302534.776712/1‑94 (MQ=60)

                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGA                                                                            <  SRR4302534.2437793/94‑1 (MQ=60)

                 GGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGA                                                                            <  SRR4302534.723011/94‑1 (MQ=60)

                  GTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAA                                                                           >  SRR4302534.414531/1‑94 (MQ=60)

                  GTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAA                                                                           <  SRR4302534.2731995/94‑1 (MQ=60)

                    ACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAG                                                                         >  SRR4302534.59467/1‑94 (MQ=60)

                     CGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGGAAGG                                                                        >  SRR4302534.403728/1‑94 (MQ=60)

                     CGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGG                                                                        >  SRR4302534.904229/1‑94 (MQ=60)

                      GCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGT                                                                       >  SRR4302534.1930061/1‑94 (MQ=60)

                       CCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGA                                                                      >  SRR4302534.5267265/1‑94 (MQ=60)

                        CGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTAT                                                                     <  SRR4302534.3971811/94‑1 (MQ=60)

                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                    <  SRR4302534.573970/94‑1 (MQ=60)

                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATC                                                                    >  SRR4302534.6591478/1‑94 (MQ=60)

                         GGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGCATC                                                                    >  SRR4302534.3002580/1‑94 (MQ=60)

                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                   <  SRR4302534.5181913/94‑1 (MQ=60)

                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                   <  SRR4302534.4776194/94‑1 (MQ=60)

                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                   <  SRR4302534.2852930/94‑1 (MQ=60)

                          GTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCG                                                                   <  SRR4302534.6578934/94‑1 (MQ=60)

                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAACAGTATCGG                                                                  >  SRR4302534.5573124/1‑94 (MQ=60)

                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGG                                                                  <  SRR4302534.889115/94‑1 (MQ=60)

                           TAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGCATGGCTGCGAAAGGTATCGG                                                                  >  SRR4302534.5652237/1‑94 (MQ=60)

                           TAGACATCGTACTGAACCCGCTGGGCGTAACGTCTCGTATGAACATAGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGgacggggtagg                                                                  >  SRR4302534.2221540/1‑83 (MQ=60)

                            AGACATCGTCCTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCGGC                                                                 <  SRR4302534.5666372/94‑1 (MQ=60)

                            AGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGC                                                                 >  SRR4302534.4953911/1‑94 (MQ=60)

                            AGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGC                                                                 >  SRR4302534.683498/1‑94 (MQ=60)

                             GACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCG                                                                <  SRR4302534.6037348/94‑1 (MQ=60)

                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGCAAGGTATCGGCGA                                                               >  SRR4302534.285551/1‑94 (MQ=60)

                              ACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGA                                                               >  SRR4302534.4369838/1‑94 (MQ=60)

                               CATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGAC                                                              <  SRR4302534.5822948/94‑1 (MQ=60)

                                ATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACA                                                             <  SRR4302534.4099112/94‑1 (MQ=60)

                                  CGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAG                                                           >  SRR4302534.2939834/1‑94 (MQ=60)

                                   GTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGACACGTATCAGCAACAAGA                                                          >  SRR4302534.5671179/1‑94 (MQ=60)

                                   GTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGA                                                          <  SRR4302534.2640364/94‑1 (MQ=60)

                                    TACTGATCCCGCTGGGCGTTCGGTCTCGTATGAACATCGGTCAGATCTTCGAAGCCCGCCTGGGTATGGCTGCGAAGGGTAGCGGCGACAAGAT                                                         <  SRR4302534.2191862/94‑1 (MQ=60)

                                    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAT                                                         <  SRR4302534.4046736/94‑1 (MQ=60)

                                    TACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAT                                                         <  SRR4302534.364089/94‑1 (MQ=60)

                                     TCTGACCCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                        <  SRR4302534.1618565/94‑1 (MQ=60)

                                     ACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATC                                                        <  SRR4302534.3633772/94‑1 (MQ=60)

                                      ctgacccatctgggtgtatcgtgtaaTATGAACATCGGACTGATACTCGAACCCCGCCTGGGTATGGCTGCGAAAAGTATCGGCGACAAGATCA                                                       <  SRR4302534.6610974/68‑1 (MQ=40)

                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                       <  SRR4302534.4439213/94‑1 (MQ=60)

                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                       <  SRR4302534.6320709/94‑1 (MQ=60)

                                      CTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCA                                                       <  SRR4302534.4762371/94‑1 (MQ=60)

                                       TGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAA                                                      <  SRR4302534.5861814/94‑1 (MQ=60)

                                        GAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCTGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGCATCGCCGACAAGcaccac                                                     >  SRR4302534.2681752/1‑88 (MQ=60)

                                        GAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAC                                                     >  SRR4302534.4165249/1‑94 (MQ=60)

                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                    >  SRR4302534.6461191/1‑94 (MQ=60)

                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                    >  SRR4302534.6187472/1‑94 (MQ=60)

                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                    <  SRR4302534.2216157/94‑1 (MQ=60)

                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACG                                                    >  SRR4302534.2857176/1‑94 (MQ=60)

                                         AACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAACAACG                                                    >  SRR4302534.4551863/1‑94 (MQ=60)

                                           CCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCC                                                  <  SRR4302534.2661794/94‑1 (MQ=60)

                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                 >  SRR4302534.444013/1‑94 (MQ=60)

                                            CCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCA                                                 <  SRR4302534.2693992/94‑1 (MQ=60)

                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                >  SRR4302534.2431900/1‑94 (MQ=60)

                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAT                                                <  SRR4302534.6535548/94‑1 (MQ=60)

                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAA                                                <  SRR4302534.925930/94‑1 (MQ=60)

                                             CGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAA                                                <  SRR4302534.3309130/94‑1 (MQ=60)

                                              GCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACTCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATG                                               >  SRR4302534.6641493/1‑94 (MQ=60)

                                               CTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGC                                              >  SRR4302534.3216514/1‑94 (MQ=60)

                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                             >  SRR4302534.6562920/1‑94 (MQ=60)

                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                             >  SRR4302534.3582464/1‑94 (MQ=60)

                                                TGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCT                                             <  SRR4302534.4625598/94‑1 (MQ=60)

                                                 GGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTG                                            >  SRR4302534.4777838/1‑94 (MQ=60)

                                                  GGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGA                                           <  SRR4302534.4142982/94‑1 (MQ=60)

                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAA                                         >  SRR4302534.5951920/1‑94 (MQ=60)

                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAA                                         >  SRR4302534.309167/1‑94 (MQ=60)

                                                    CGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAA                                         >  SRR4302534.3251252/1‑94 (MQ=60)

                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCGTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAA                                        >  SRR4302534.6443660/1‑94 (MQ=60)

                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGACAC                                        >  SRR4302534.1059915/1‑94 (MQ=60)

                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                        <  SRR4302534.4091108/94‑1 (MQ=60)

                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                        <  SRR4302534.153327/94‑1 (MQ=60)

                                                     GTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAC                                        <  SRR4302534.3765683/94‑1 (MQ=60)

                                                      TACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACA                                       >  SRR4302534.2152324/1‑94 (MQ=60)

                                                      TACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACA                                       >  SRR4302534.4070825/1‑94 (MQ=60)

                                                       ACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAG                                      <  SRR4302534.3060473/94‑1 (MQ=60)

                                                         CGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCA                                    >  SRR4302534.4182003/1‑94 (MQ=60)

                                                          GTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAG                                   <  SRR4302534.5812856/94‑1 (MQ=60)

                                                            CTCGTATGAACATCGGTCAGGTCCTCGCAACCCACCTGGGTATAGCTGAGAAAGGCATCCGCGACAAGATCAACGCCGTGGTGAAACAGCAGCC                                 >  SRR4302534.2852042/1‑94 (MQ=60)

                                                            CTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCCACGCCATGCTGAAACAGCAGCA                                 >  SRR4302534.2675225/1‑94 (MQ=60)

                                                            CTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCA                                 <  SRR4302534.1027384/94‑1 (MQ=60)

                                                             TCGTATGAGCATCGGTCAGATCCTCGAATCCCGACTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAA                                <  SRR4302534.3291339/94‑1 (MQ=60)

                                                             TCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAA                                <  SRR4302534.199209/94‑1 (MQ=60)

                                                             TCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAA                                >  SRR4302534.1713346/1‑94 (MQ=60)

                                                               GTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                              >  SRR4302534.1187595/1‑94 (MQ=60)

                                                               GTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                              <  SRR4302534.1886350/94‑1 (MQ=60)

                                                               GTATGAACATCGGTCAGAGCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGA                              <  SRR4302534.4430688/94‑1 (MQ=60)

                                                                TATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAA                             >  SRR4302534.173248/1‑94 (MQ=60)

                                                                TATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAA                             >  SRR4302534.1588269/1‑94 (MQ=60)

                                                                TATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAA                             <  SRR4302534.2903991/94‑1 (MQ=60)

                                                                GATGAACCTCGGTCAGAGCCGCGAACCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAA                             <  SRR4302534.4680633/94‑1 (MQ=60)

                                                                 ATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                            <  SRR4302534.5477242/94‑1 (MQ=60)

                                                                 ATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                            <  SRR4302534.4760592/94‑1 (MQ=60)

                                                                 ATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAG                            <  SRR4302534.1545734/94‑1 (MQ=60)

                                                                   GAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                          <  SRR4302534.2090039/94‑1 (MQ=60)

                                                                   GAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTC                          <  SRR4302534.1756737/94‑1 (MQ=60)

                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                         >  SRR4302534.2329536/1‑94 (MQ=60)

                                                                    AACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCG                         >  SRR4302534.5068131/1‑94 (MQ=60)

                                                                     ACATCGGTCAGATCCTCGAAACGCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGC                        >  SRR4302534.4202042/1‑94 (MQ=60)

                                                                     ACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGC                        >  SRR4302534.1790190/1‑94 (MQ=60)

                                                                     ACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGC                        <  SRR4302534.938691/94‑1 (MQ=60)

                                                                      CATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                       >  SRR4302534.1286350/1‑94 (MQ=60)

                                                                      CATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCG                       >  SRR4302534.1117140/1‑94 (MQ=60)

                                                                       ATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGA                      <  SRR4302534.4130913/94‑1 (MQ=60)

                                                                       ATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGA                      <  SRR4302534.4040523/94‑1 (MQ=60)

                                                                       ATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGA                      >  SRR4302534.913508/1‑94 (MQ=60)

                                                                         CGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAA                    >  SRR4302534.3840938/1‑94 (MQ=60)

                                                                          GGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAC                   <  SRR4302534.3236214/94‑1 (MQ=60)

                                                                           GTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACT                  <  SRR4302534.4645001/94‑1 (MQ=60)

                                                                            TCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTG                 <  SRR4302534.3765006/94‑1 (MQ=60)

                                                                             CAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGC                >  SRR4302534.4997669/1‑94 (MQ=60)

                                                                             CAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGC                <  SRR4302534.4941407/94‑1 (MQ=60)

                                                                              AGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCG               >  SRR4302534.283376/1‑94 (MQ=60)

                                                                              AGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCG               <  SRR4302534.5291562/94‑1 (MQ=60)

                                                                              AGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGAAAGAAGCCGCGAAACTGCG               >  SRR4302534.1200897/1‑94 (MQ=60)

                                                                                ATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCG             <  SRR4302534.5345435/94‑1 (MQ=60)

                                                                                 TCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGA            >  SRR4302534.4633866/1‑94 (MQ=60)

                                                                                 TCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGA            >  SRR4302534.2496162/1‑94 (MQ=60)

                                                                                  CCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA           >  SRR4302534.5684407/1‑94 (MQ=60)

                                                                                  CCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAA           >  SRR4302534.2661535/1‑94 (MQ=60)

                                                                                    TCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATT         >  SRR4302534.1290169/1‑94 (MQ=60)

                                                                                     CGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC        <  SRR4302534.770019/94‑1 (MQ=60)

                                                                                      GAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCA       <  SRR4302534.6416926/94‑1 (MQ=60)

                                                                                       AAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCAT      >  SRR4302534.1387110/1‑94 (MQ=60)

                                                                                        AACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC     <  SRR4302534.4328898/94‑1 (MQ=60)

                                                                                         ACCCGCCGGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCC    <  SRR4302534.1186392/94‑1 (MQ=60)

                                                                                          CCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCA   >  SRR4302534.4308149/1‑94 (MQ=60)

                                                                                           CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  <  SRR4302534.3091607/94‑1 (MQ=60)

                                                                                           CCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  <  SRR4302534.1864187/94‑1 (MQ=60)

TGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4182076‑4182260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`