Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,949 +G intergenic (+82/‑76) nusG → / → rplK NusG/50S ribosomal subunit protein L11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,9471.G99.5% 727.0 / ‑4.0 210intergenic (+80/‑78)nusG/rplKNusG/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (102/107);  total (102/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 228 total aligned reads displayed.

TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAA  >  NC_000913/4175855‑4176036
                                                                                             |                                                                                          
tGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                                                                                             <  2:4304845/93‑1 (MQ=255)
 gAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑g                                                                                           <  2:159464/93‑1 (MQ=255)
  aaaaaGCCTAACCCAGTGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gg                                                                                          <  1:4777096/93‑1 (MQ=255)
   aaaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACACTTTCGCGCCTTTTGTTTTTAT‑ggg                                                                                         >  1:600823/1‑92 (MQ=255)
    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                        >  2:6225455/1‑91 (MQ=255)
    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                        <  1:1956862/93‑3 (MQ=255)
    aaaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggc                                                                                        >  2:3244240/1‑91 (MQ=255)
     aaGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑gggcc                                                                                       >  2:375893/1‑90 (MQ=255)
        ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                                                                                     <  1:509501/93‑1 (MQ=255)
        ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                                                                                     <  2:3977943/93‑1 (MQ=255)
        ccTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                                                                                     <  1:6214024/93‑1 (MQ=255)
         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                    <  1:5965273/93‑1 (MQ=255)
         cTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGc                                                                                    >  1:2077351/1‑93 (MQ=255)
           aaCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGccc                                                                                  >  1:5297729/1‑93 (MQ=255)
               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaa                                                                              >  2:5601981/1‑93 (MQ=255)
               cAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaa                                                                              >  2:2888220/1‑93 (MQ=255)
                aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa                                                                             <  2:5375367/93‑1 (MQ=255)
                aGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa                                                                             >  2:2703601/1‑93 (MQ=255)
                   gATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑Cg                                                                         >  2:1080111/1‑93 (MQ=255)
                   gATCAAAAAAGCGGCGATTCAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑Cg                                                                         >  1:6284052/1‑93 (MQ=255)
                    aTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGa                                                                        >  1:5772745/1‑93 (MQ=255)
                      cAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAtt                                                                      <  1:1262955/93‑1 (MQ=255)
                        aaaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAtttt                                                                    >  2:3384931/1‑93 (MQ=255)
                         aaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAttttt                                                                   <  1:4458196/93‑1 (MQ=255)
                         aaaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAttttt                                                                   >  2:1219268/1‑93 (MQ=255)
                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAtttttt                                                                  >  2:1336486/1‑93 (MQ=255)
                          aaaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGAtttttt                                                                  <  1:6176214/93‑1 (MQ=255)
                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTta                                                                 <  1:855160/93‑1 (MQ=255)
                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTta                                                                 <  1:1470311/93‑1 (MQ=255)
                           aaGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTta                                                                 <  2:1775564/93‑1 (MQ=255)
                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTtat                                                                >  1:6271782/1‑93 (MQ=255)
                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTtat                                                                <  1:3359717/93‑1 (MQ=255)
                            aGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTtat                                                                >  2:5733221/1‑93 (MQ=255)
                             gcggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTtata                                                               <  1:451594/93‑1 (MQ=255)
                              cggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTatat                                                              >  2:5499305/1‑93 (MQ=255)
                               ggcgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATc                                                             >  1:2851465/1‑93 (MQ=255)
                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCAc                                                           <  2:3295117/93‑1 (MQ=255)
                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCAc                                                           >  1:5634512/1‑93 (MQ=255)
                                 cgATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCAc                                                           <  2:6455167/93‑1 (MQ=255)
                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACg                                                          >  1:119489/1‑93 (MQ=255)
                                  gATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACg                                                          >  2:3122837/1‑93 (MQ=255)
                                   aTTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACgg                                                         <  1:1660548/93‑1 (MQ=255)
                                     ttAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACgggg                                                       <  1:4420954/93‑1 (MQ=255)
                                          cGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCct                                                  <  1:2329727/93‑1 (MQ=255)
                                            ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGTGGAGCctct                                                >  2:3602614/1‑93 (MQ=255)
                                            ttGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCctct                                                <  1:2652366/93‑1 (MQ=255)
                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCaga                                            >  1:2724354/1‑93 (MQ=255)
                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCaga                                            <  1:2905996/93‑1 (MQ=255)
                                                acaAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCaga                                            <  2:1466579/93‑1 (MQ=255)
                                                 caAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCagag                                           <  1:3095434/93‑1 (MQ=255)
                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                         <  1:5397722/93‑1 (MQ=255)
                                                   aGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGc                                         >  1:1843601/1‑93 (MQ=255)
                                                     gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGt                                       <  1:3893853/93‑1 (MQ=255)
                                                     gCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGt                                       <  2:2789581/93‑1 (MQ=255)
                                                       gTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGtta                                     <  1:2936017/93‑1 (MQ=255)
                                                        tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttat                                    <  2:6081952/93‑1 (MQ=255)
                                                        tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttat                                    >  2:3513647/1‑93 (MQ=255)
                                                        tGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttat                                    >  1:4717860/1‑93 (MQ=255)
                                                         gagaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt                                   <  1:2211653/93‑1 (MQ=255)
                                                         gagaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGttatt                                   <  1:5916093/93‑1 (MQ=255)
                                                           gaTTGGAATACAATTTCGCGCGTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGCGGAGCCTCTCAGAGGCGTTATTAc                                 <  1:6225455/93‑1 (MQ=255)
                                                           gaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                                 <  1:1304686/93‑1 (MQ=255)
                                                           gaTTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAc                                 <  1:3618492/93‑1 (MQ=255)
                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                              >  1:3999841/1‑93 (MQ=255)
                                                              tGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCa                              <  1:1530672/93‑1 (MQ=255)
                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                            <  2:4328964/93‑1 (MQ=255)
                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                            <  1:2026465/93‑1 (MQ=255)
                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                            >  2:883088/1‑93 (MQ=255)
                                                                gAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAc                            >  2:1818548/1‑93 (MQ=255)
                                                                 aaTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACt                           <  2:1245263/93‑1 (MQ=255)
                                                                  aTACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACtt                          <  2:4845300/93‑1 (MQ=255)
                                                                   tACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTg                         >  2:2043766/1‑93 (MQ=255)
                                                                    aCAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                        <  1:2284781/93‑1 (MQ=255)
                                                                    aCAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGa                        <  1:3518684/93‑1 (MQ=255)
                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                       <  1:6450970/93‑1 (MQ=255)
                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                       >  1:6420932/1‑93 (MQ=255)
                                                                     cAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAg                       <  1:1539874/93‑1 (MQ=255)
                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                     >  2:2108964/1‑93 (MQ=255)
                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                     >  1:4866945/1‑93 (MQ=255)
                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                     >  2:6460763/1‑93 (MQ=255)
                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                     <  2:5106632/93‑1 (MQ=255)
                                                                       aTTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGa                     >  1:5082945/1‑93 (MQ=255)
                                                                         ttCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAt                   <  1:4028789/93‑1 (MQ=255)
                                                                           cgcgCCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAAttt                 <  2:1912342/93‑1 (MQ=255)
                                                                           agcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACGCAACTGGAGAAAttt                 >  1:189284/2‑93 (MQ=255)
                                                                            gcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta                >  1:1015385/1‑93 (MQ=255)
                                                                            gcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta                <  1:3426344/93‑1 (MQ=255)
                                                                            gcgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTta                >  1:4956822/1‑93 (MQ=255)
                                                                             cgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat               <  2:4710111/93‑1 (MQ=255)
                                                                             cgcCTTTTGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTtat               >  2:1638124/1‑93 (MQ=255)
                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg           <  1:6171031/93‑1 (MQ=255)
                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg           >  2:5158320/1‑93 (MQ=255)
                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg           <  2:3634542/93‑1 (MQ=255)
                                                                                 ttttGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATg           <  1:512645/93‑1 (MQ=255)
                                                                                    tGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCt        <  2:545928/93‑1 (MQ=255)
                                                                                    tGTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCt        <  2:1025771/93‑1 (MQ=255)
                                                                                     gTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTCTATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTa       >  1:2531324/1‑93 (MQ=255)
                                                                                     gTTTTTATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTa       <  1:1087884/93‑1 (MQ=255)
                                                                                      tttttATGGGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTaa      <  1:3844543/93‑1 (MQ=255)
                                                                                          ttt‑GGCCCTGCCCCTAAAACCGTTTTTTTATTCCCGGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGaaa  <  1:2775334/91‑1 (MQ=255)
                                                                                             |                                                                                          
TGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCTTGCCCGTAAAA‑CGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAA  >  NC_000913/4175855‑4176036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042
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GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG                                                                                                <  SRR4302534.2333798/94‑1 (MQ=60)
 AAAAAGCCTAACCCAGTGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑G                                                                                              <  SRR4302534.4906819/94‑1 (MQ=60)
 AAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACACTTTCGCGCCTTTTGTTTTTAT‑GG‑G                                                                                              >  SRR4302534.615468/1‑94 (MQ=60)
  AAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑GC                                                                                             >  SRR4302534.4562167/1‑94 (MQ=60)
  AAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑GC                                                                                             <  SRR4302534.1304089/94‑1 (MQ=60)
   AAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgcc                                                                                             <  SRR4302534.2008070/94‑4 (MQ=60)
   AAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGgcc                                                                                             >  SRR4302534.1311663/1‑91 (MQ=60)
     AGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTT                                                                                           >  SRR4302534.1878337/1‑94 (MQ=60)
      GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTG                                                                                          <  SRR4302534.62113/94‑1 (MQ=60)
      GCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTG                                                                                          <  SRR4302534.4005311/94‑1 (MQ=60)
       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGC                                                                                         >  SRR4302534.2131736/1‑94 (MQ=60)
       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGC                                                                                         <  SRR4302534.6382871/94‑1 (MQ=60)
       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGC                                                                                         <  SRR4302534.6382872/94‑1 (MQ=60)
       CCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGC                                                                                         <  SRR4302534.522184/94‑1 (MQ=60)
        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                        <  SRR4302534.6123424/94‑1 (MQ=60)
        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                        >  SRR4302534.2708338/1‑94 (MQ=60)
        CTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCC                                                                                        <  SRR4302534.6127266/94‑1 (MQ=60)
         TAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCC                                                                                       >  SRR4302534.5440711/1‑94 (MQ=60)
          AACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCG                                                                                      <  SRR4302534.2728956/94‑1 (MQ=60)
          AACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCG                                                                                      >  SRR4302534.2721931/1‑94 (MQ=60)
           ACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGT                                                                                     >  SRR4302534.4708562/1‑94 (MQ=60)
              CAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAA                                                                                  >  SRR4302534.6182433/1‑94 (MQ=60)
              CAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAA                                                                                  >  SRR4302534.1129406/1‑94 (MQ=60)
                GCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATCCAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAAC                                                                                <  SRR4302534.6442801/94‑1 (MQ=60)
                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                               >  SRR4302534.3039770/1‑94 (MQ=60)
                 CGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                               >  SRR4302534.5001163/1‑94 (MQ=60)
                 CGATCAAAAAAGCGGCGATTCAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                               >  SRR4302534.6454081/1‑94 (MQ=60)
                 CGATCAAAAAAGCGGCGATTAAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACG                                                                               >  SRR4302534.6461427/1‑94 (MQ=60)
                  GATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGA                                                                              >  SRR4302534.5929354/1‑94 (MQ=60)
                   ATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGAT                                                                             >  SRR4302534.3878491/1‑94 (MQ=60)
                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTT                                                                           <  SRR4302534.110193/94‑1 (MQ=60)
                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTT                                                                           <  SRR4302534.1296231/94‑1 (MQ=60)
                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTT                                                                           >  SRR4302534.640484/1‑94 (MQ=60)
                     CAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTGTGGTTTTATGGG‑CCTTGCCCGTAAAACGATTT                                                                           >  SRR4302534.2250116/1‑94 (MQ=60)
                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                          >  SRR4302534.1543910/1‑94 (MQ=60)
                      AAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTT                                                                          <  SRR4302534.2985524/94‑1 (MQ=60)
                       AAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTT                                                                         >  SRR4302534.3381770/1‑94 (MQ=60)
                       AAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTT                                                                         >  SRR4302534.1579116/1‑94 (MQ=60)
                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                        <  SRR4302534.4578722/94‑1 (MQ=60)
                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                        >  SRR4302534.3959790/1‑94 (MQ=60)
                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                        >  SRR4302534.5222566/1‑94 (MQ=60)
                        AAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTT                                                                        >  SRR4302534.347679/1‑94 (MQ=60)
                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       <  SRR4302534.6344285/94‑1 (MQ=60)
                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       <  SRR4302534.6344286/94‑1 (MQ=60)
                         AAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTA                                                                       >  SRR4302534.6180119/1‑94 (MQ=60)
                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      >  SRR4302534.2819494/1‑94 (MQ=60)
                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      >  SRR4302534.6441585/1‑94 (MQ=60)
                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302534.876736/94‑1 (MQ=60)
                          AAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTAT                                                                      <  SRR4302534.1508989/94‑1 (MQ=60)
                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     >  SRR4302534.4619153/1‑94 (MQ=60)
                           AGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATA                                                                     <  SRR4302534.3449705/94‑1 (MQ=60)
                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                    <  SRR4302534.463033/94‑1 (MQ=60)
                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                    <  SRR4302534.4298670/94‑1 (MQ=60)
                            GCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATAT                                                                    >  SRR4302534.1942958/1‑94 (MQ=60)
                             CGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATC                                                                   >  SRR4302534.2926377/1‑94 (MQ=60)
                              GGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCA                                                                  <  SRR4302534.5405739/94‑1 (MQ=60)
                               GCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCAC                                                                 >  SRR4302534.5787013/1‑94 (MQ=60)
                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                                >  SRR4302534.2508142/1‑94 (MQ=60)
                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                                >  SRR4302534.122661/1‑94 (MQ=60)
                                CGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACG                                                                <  SRR4302534.5400973/94‑1 (MQ=60)
                                  ATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGG                                                              <  SRR4302534.1703801/94‑1 (MQ=60)
                                    TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                            <  SRR4302534.4540309/94‑1 (MQ=60)
                                    TTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGA                                                            <  SRR4302534.206894/94‑1 (MQ=60)
                                     TAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAG                                                           <  SRR4302534.1404692/94‑1 (MQ=60)
                                       ATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCC                                                         >  SRR4302534.817745/1‑94 (MQ=60)
                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       <  SRR4302534.2391538/94‑1 (MQ=60)
                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       <  SRR4302534.6597763/94‑1 (MQ=60)
                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302534.48635/1‑94 (MQ=60)
                                         CGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTC                                                       >  SRR4302534.1985075/1‑94 (MQ=60)
                                           TTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTC                                                     <  SRR4302534.2722510/94‑1 (MQ=60)
                                           GTGCACAAGCCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CTTTGCCCGTAAAACGATTTTTTATATCGCGGGGAGCCTCTC                                                     <  SRR4302534.1786887/94‑1 (MQ=60)
                                            TGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCA                                                    <  SRR4302534.4119988/94‑1 (MQ=60)
                                              CACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGA                                                  >  SRR4302534.2796225/1‑94 (MQ=60)
                                               ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 <  SRR4302534.2982288/94‑1 (MQ=60)
                                               ACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAG                                                 >  SRR4302534.4801084/1‑94 (MQ=60)
                                                CAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGG                                                <  SRR4302534.2344619/94‑1 (MQ=60)
                                                CAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGG                                                <  SRR4302534.324795/94‑1 (MQ=60)
                                                CAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGG                                                <  SRR4302534.3176867/94‑1 (MQ=60)
                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               >  SRR4302534.1891512/1‑94 (MQ=60)
                                                 AAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGC                                               >  SRR4302534.4429129/1‑94 (MQ=60)
                                                  AGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCG                                              >  SRR4302534.1291586/1‑94 (MQ=60)
                                                  AGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCG                                              <  SRR4302534.5543049/94‑1 (MQ=60)
                                                   GGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGT                                             <  SRR4302534.3686107/94‑1 (MQ=60)
                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTT                                            <  SRR4302534.6004850/94‑1 (MQ=60)
                                                    GCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTT                                            <  SRR4302534.3998368/94‑1 (MQ=60)
                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGCGGCGTTA                                           >  SRR4302534.3568272/1‑94 (MQ=60)
                                                     CGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTA                                           >  SRR4302534.2348956/1‑94 (MQ=60)
                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                          <  SRR4302534.5110176/94‑1 (MQ=60)
                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                          <  SRR4302534.3013076/94‑1 (MQ=60)
                                                      GTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTAT                                          >  SRR4302534.4845976/1‑94 (MQ=60)
                                                       TGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATT                                         <  SRR4302534.2886930/94‑1 (MQ=60)
                                                        GAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTA                                        <  SRR4302534.2270226/94‑1 (MQ=60)
                                                        GAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTA                                        <  SRR4302534.6076740/94‑1 (MQ=60)
                                                         AGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       <  SRR4302534.6435016/94‑1 (MQ=60)
                                                         AGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTAC                                       >  SRR4302534.1682152/1‑94 (MQ=60)
                                                          GATTGGAATACAATTTCGCGCGTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGCGGAGCCTCTCAGAGGCGTTATTACC                                      <  SRR4302534.6394481/94‑1 (MQ=60)
                                                          GATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACC                                      <  SRR4302534.1339104/94‑1 (MQ=60)
                                                          GATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACC                                      <  SRR4302534.3715777/94‑1 (MQ=60)
                                                           ATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCC                                     >  SRR4302534.2968684/1‑94 (MQ=60)
                                                            TTGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    >  SRR4302534.4106912/1‑94 (MQ=60)
                                                            TTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GGCCCTTGCCCGTAAAACGATTTTTTATATCCCGGGGAGCCTCTCAGAGGCGTTATTACCCA                                    <  SRR4302534.2221391/94‑1 (MQ=60)
                                                             TGGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAA                                   <  SRR4302534.1570831/94‑1 (MQ=60)
                                                              GGAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAAC                                  >  SRR4302534.4924430/1‑94 (MQ=60)
                                                               GAATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACT                                 <  SRR4302534.2079477/94‑1 (MQ=60)
                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                <  SRR4302534.5532416/94‑1 (MQ=60)
                                                                AATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTT                                <  SRR4302534.3757927/94‑1 (MQ=60)
                                                                 ATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               >  SRR4302534.5569149/1‑94 (MQ=60)
                                                                 ATACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTG                               >  SRR4302534.5867954/1‑94 (MQ=60)
                                                                  TACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGA                              <  SRR4302534.4042416/94‑1 (MQ=60)
                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             >  SRR4302534.6594123/1‑94 (MQ=60)
                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302534.3613321/94‑1 (MQ=60)
                                                                   ACAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAG                             <  SRR4302534.2345415/94‑1 (MQ=60)
                                                                    CAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGG                            <  SRR4302534.1580239/94‑1 (MQ=60)
                                                                    CAATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGG                            <  SRR4302534.6624814/94‑1 (MQ=60)
                                                                     AATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA                           >  SRR4302534.4998965/1‑94 (MQ=60)
                                                                     AATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGA                           >  SRR4302534.5220135/1‑94 (MQ=60)
                                                                      ATTTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATAACGGGGAGCCTCTCAGAGGCGTTATTACCCAccttaaggat                          >  SRR4302534.832437/1‑84 (MQ=60)
                                                                        TTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATT                        <  SRR4302534.4136802/94‑1 (MQ=60)
                                                                        TTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATT                        <  SRR4302534.1669168/94‑1 (MQ=60)
                                                                        TTCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATT                        <  SRR4302534.5245728/94‑1 (MQ=60)
                                                                         TCGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTT                       <  SRR4302534.6164542/94‑1 (MQ=60)
                                                                         TAGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACGCAACTGGAGAAATTT                       >  SRR4302534.194255/1‑94 (MQ=60)
                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      >  SRR4302534.1041524/1‑94 (MQ=60)
                                                                          CGCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTA                      >  SRR4302534.5091061/1‑94 (MQ=60)
                                                                           GCGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTAT                     <  SRR4302534.3518400/94‑1 (MQ=60)
                                                                            CGCCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATA                    >  SRR4302534.603052/1‑94 (MQ=60)
                                                                              CCTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAAT                  >  SRR4302534.2091682/1‑94 (MQ=60)
                                                                               CTTTTTTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 <  SRR4302534.5114444/94‑1 (MQ=60)
                                                                               CTTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATG                 >  SRR4302534.1893890/1‑94 (MQ=60)
                                                                                TTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG                <  SRR4302534.6338895/94‑1 (MQ=60)
                                                                                TTTTGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGG                <  SRR4302534.525406/94‑1 (MQ=60)
                                                                                   TGTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTCTATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTA             >  SRR4302534.2598487/1‑94 (MQ=60)
                                                                                    GTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAA            <  SRR4302534.293770/94‑1 (MQ=60)
                                                                                    GTTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAA            <  SRR4302534.1116181/94‑1 (MQ=60)
                                                                                     TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG           <  SRR4302534.3947797/94‑1 (MQ=60)
                                                                                     TTTTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAG           <  SRR4302534.882528/94‑1 (MQ=60)
                                                                                       TTTATGGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAA         >  SRR4302534.4071871/1‑94 (MQ=60)
                                                                                          atgGG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGT      >  SRR4302534.1682172/4‑94 (MQ=60)
                                                                                           G‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    <  SRR4302534.439461/94‑1 (MQ=60)
                                                                                           G‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTAC    >  SRR4302534.3473745/1‑94 (MQ=60)
                                                                                             GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACA   >  SRR4302534.5787227/1‑94 (MQ=60)
                                                                                              G‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGAGTTATTACCGCACTTGAGGAATCTATAATGGCTAAGAAAGTACAA  >  SRR4302534.710248/1‑94 (MQ=60)
                                                                                              |                                                                                              
GAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAT‑GG‑CCTTGCCCGTAAAACGATTTTTTATATCACGGGGAGCCTCTCAGAGGCGTTATTACCCAACTTGAGGAATTTATAATGGCTAAGAAAGTACAA  >  NC_000913/4175856‑4176042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑