Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,532,411 A→G intergenic (‑1059/‑172) yjhQ ← / → yjhR hypothetical protein/putative frameshift suppressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,532,4110AG99.4% 1115.9 / ‑3.7 326intergenic (‑1059/‑172)yjhQ/yjhRhypothetical protein/putative frameshift suppressor
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (174/148);  total (177/149)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 336 total aligned reads displayed.

CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA  >  NC_000913/4532321‑4532500
                                                                                          |                                                                                         
cTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCggg                                                                                         <  2:780280/93‑1 (MQ=255)
cTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCggg                                                                                         <  1:255420/93‑1 (MQ=255)
 tGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGt                                                                                        >  1:786347/1‑93 (MQ=255)
 tGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCT‑AAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTa                                                                                       <  2:6950407/93‑1 (MQ=38)
    ccACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGc                                                                                     >  1:5451413/1‑93 (MQ=255)
    ccACGATTAAAGGCGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGAGTACTGCGGGTAGc                                                                                     >  2:3813660/1‑93 (MQ=255)
     cACGATTAAAGGTGGTCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  2:340943/1‑93 (MQ=255)
     cACGATTAAAGGTGGTCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  1:3839896/1‑93 (MQ=255)
     cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  1:1179838/1‑93 (MQ=255)
     cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  1:2912127/1‑93 (MQ=255)
      ccGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGccc                                                                                   <  2:6550973/92‑1 (MQ=255)
       cGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCa                                                                                  <  2:8347985/93‑1 (MQ=255)
        gATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAg                                                                                 <  1:6941955/93‑1 (MQ=255)
         aTTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGt                                                                                <  1:7436563/93‑1 (MQ=255)
          ttAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGtt                                                                               >  2:6326015/1‑93 (MQ=255)
           tAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTc                                                                              <  2:2292428/93‑1 (MQ=255)
           tAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTc                                                                              <  2:1347734/93‑1 (MQ=255)
             aaGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCaa                                                                            >  1:7035192/1‑93 (MQ=255)
              aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAc                                                                           >  1:5347745/1‑93 (MQ=255)
              aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAc                                                                           >  1:4360828/1‑93 (MQ=255)
              aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAc                                                                           >  1:1463579/1‑93 (MQ=255)
               ggTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAct                                                                          <  2:3900954/93‑1 (MQ=255)
               ggTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAACGTAATGCGGGTAGCCCAGTTCAAct                                                                          >  2:6701629/1‑93 (MQ=255)
                 tGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAActct                                                                        >  1:6260320/1‑93 (MQ=255)
                 tGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAActct                                                                        <  2:5856917/93‑1 (MQ=255)
                     cGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACt                                                                    >  1:480922/1‑93 (MQ=255)
                      gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTa                                                                   >  1:6485760/1‑93 (MQ=255)
                      gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTa                                                                   >  2:8203589/1‑93 (MQ=255)
                        tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAcc                                                                 >  2:5041605/1‑93 (MQ=255)
                         cAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCa                                                                >  2:269093/1‑93 (MQ=255)
                         cAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCa                                                                <  2:7973019/93‑1 (MQ=255)
                          aGGCGGTCACCGTATTCGATAATAAATGGGTCTGGATAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTCCCAg                                                               >  2:7364290/1‑93 (MQ=255)
                          aGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAg                                                               >  1:6025043/1‑93 (MQ=255)
                          aGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAg                                                               <  1:3297618/93‑1 (MQ=255)
                            gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                             <  1:5304132/93‑1 (MQ=255)
                             cGGTCACCGTATTCGATAATAACTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCCACTCTCACTACCAGTag                                                            >  1:5174058/1‑92 (MQ=255)
                             cGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAt                                                            <  1:902694/93‑1 (MQ=255)
                               gTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGa                                                          <  2:55929/93‑1 (MQ=255)
                                  aCCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATcc                                                       >  2:4435872/1‑93 (MQ=255)
                                   ccGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                      >  2:2507897/1‑93 (MQ=255)
                                   ccGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                      >  1:6664673/1‑93 (MQ=255)
                                     gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                    >  1:2806420/1‑93 (MQ=255)
                                     gTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCaa                                                    >  1:1435884/1‑93 (MQ=255)
                                      tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                   >  1:3035182/1‑93 (MQ=255)
                                       aTTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGt                                                  <  2:1628419/93‑1 (MQ=255)
                                       aTTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGt                                                  <  1:5643839/93‑1 (MQ=255)
                                        ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                 >  2:749484/1‑93 (MQ=255)
                                           gATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGa                                              <  1:3456881/93‑1 (MQ=255)
                                           gATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGa                                              <  1:8293374/93‑1 (MQ=255)
                                            ataataAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAg                                             <  2:6162350/93‑1 (MQ=255)
                                             taataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgc                                            <  2:3602512/93‑1 (MQ=255)
                                              aataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCACGTTcgcgcg                                           >  1:3130104/1‑93 (MQ=255)
                                                taaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                         <  2:4972477/93‑1 (MQ=255)
                                                taaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                         <  1:1980882/93‑1 (MQ=255)
                                                  aaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGa                                       <  2:1040396/93‑1 (MQ=255)
                                                   aTGGGTCTGGAAAAAGTGCAGCATCTGGCATCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGaa                                      <  1:1005384/93‑1 (MQ=255)
                                                   aTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGag                                      >  2:1941678/1‑92 (MQ=255)
                                                    tGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAt                                     >  2:6620965/1‑93 (MQ=255)
                                                     gggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATg                                    <  1:6554700/93‑1 (MQ=255)
                                                      ggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGt                                   >  1:4685455/1‑93 (MQ=255)
                                                       gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                  >  2:2781044/1‑93 (MQ=255)
                                                       gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                  >  1:5903702/1‑93 (MQ=255)
                                                       gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                  >  1:4521423/1‑93 (MQ=255)
                                                       gTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTa                                  >  1:1865030/1‑93 (MQ=255)
                                                        tCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAt                                 <  2:2379992/93‑1 (MQ=255)
                                                         cTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATc                                >  2:2051520/1‑93 (MQ=255)
                                                         cTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATc                                <  2:6469387/93‑1 (MQ=255)
                                                         cTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATc                                <  1:4006501/93‑1 (MQ=255)
                                                         cTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATc                                <  1:2262407/93‑1 (MQ=255)
                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               <  1:8505752/93‑1 (MQ=255)
                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               <  1:3594074/93‑1 (MQ=255)
                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               <  2:7174968/93‑1 (MQ=255)
                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               >  2:5087917/1‑93 (MQ=255)
                                                           ggAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTg                              <  1:2690026/93‑1 (MQ=255)
                                                            gAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGt                             >  1:4777389/1‑93 (MQ=255)
                                                             aaaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCATGTTCGAGCGCGGAATGTATCTGTa                            <  1:5060194/93‑1 (MQ=255)
                                                             aaaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTa                            <  1:4607203/93‑1 (MQ=255)
                                                              aaaaGAGCAGCATCTGGCAGCGTAATGCAGGCAGCCTAGTTTAACCTTCACTACCAGGATTATTCCAAGTTCGAGCGCGGTATGTAGCTGTAt                           >  2:8042738/1‑93 (MQ=255)
                                                                aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                         >  1:4649265/1‑93 (MQ=255)
                                                                aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                         <  1:1083627/93‑1 (MQ=255)
                                                                  gTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                       <  2:7553702/93‑1 (MQ=255)
                                                                  gTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                       >  2:1555548/1‑93 (MQ=255)
                                                                    gcagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAt                     <  1:8598960/93‑1 (MQ=255)
                                                                    gcagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAt                     <  1:1626875/93‑1 (MQ=255)
                                                                      agcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcg                   <  1:8122412/93‑1 (MQ=255)
                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  <  2:4032379/93‑1 (MQ=255)
                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  <  1:7241146/93‑1 (MQ=255)
                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  >  1:2717824/1‑93 (MQ=255)
                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  <  1:207958/93‑1 (MQ=255)
                                                                         aTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                >  1:2390716/1‑93 (MQ=255)
                                                                          tCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGc               >  2:884003/1‑93 (MQ=255)
                                                                          tCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGc               >  1:7721675/1‑93 (MQ=255)
                                                                          tCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGc               <  1:2649772/93‑1 (MQ=255)
                                                                              gCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGtt           <  2:5330157/93‑1 (MQ=255)
                                                                               cAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAATATGGTCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGttt          <  2:8664155/93‑1 (MQ=255)
                                                                                 gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa        <  2:7319057/93‑1 (MQ=255)
                                                                                 gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa        >  1:57990/1‑93 (MQ=255)
                                                                                     aaTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATaa    >  1:4933341/1‑93 (MQ=255)
                                                                                      aTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAc   <  1:8719456/93‑1 (MQ=255)
                                                                                       tGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAca  >  1:6853479/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA  >  NC_000913/4532321‑4532500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532319‑4532504
                                                                                            |                                                                                             
AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              <  SRR4302541.8749222/94‑1 (MQ=60)
 ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGGAGCATCTGGCAGCGTAATGCGGG                                                                                             >  SRR4302541.1742157/1‑94 (MQ=60)
 ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCGGCGTAATGCGGG                                                                                             >  SRR4302541.1459424/1‑94 (MQ=60)
 ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGG                                                                                             >  SRR4302541.1671611/1‑94 (MQ=60)
  CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGT                                                                                            >  SRR4302541.933058/1‑94 (MQ=60)
  CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGT                                                                                            <  SRR4302541.257493/94‑1 (MQ=60)
  CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGT                                                                                            >  SRR4302541.793645/1‑94 (MQ=60)
   TGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTA                                                                                           >  SRR4302541.2054919/1‑94 (MQ=60)
   TGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTA                                                                                           <  SRR4302541.3769790/94‑1 (MQ=60)
    GCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAG                                                                                          >  SRR4302541.4732736/1‑94 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         <  SRR4302541.5208321/94‑1 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         >  SRR4302541.2442512/1‑94 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         <  SRR4302541.5575527/94‑1 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         <  SRR4302541.5375185/94‑1 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         >  SRR4302541.5507348/1‑94 (MQ=60)
     CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGGAGC                                                                                         >  SRR4302541.3020665/1‑94 (MQ=60)
      CCACGATTAAAGGTGGTCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.3878569/1‑94 (MQ=60)
      CCACGATTAAAGGTGGTCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.3878568/1‑94 (MQ=60)
      CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.2941409/1‑94 (MQ=60)
      CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.1191241/1‑94 (MQ=60)
      CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.1191242/1‑94 (MQ=60)
      CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302541.6163787/1‑94 (MQ=60)
       CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       >  SRR4302541.4604822/1‑94 (MQ=60)
       CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       <  SRR4302541.6430948/94‑1 (MQ=60)
       CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       >  SRR4302541.2165441/1‑94 (MQ=60)
       CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       <  SRR4302541.5290110/94‑1 (MQ=60)
         CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     >  SRR4302541.4790639/1‑94 (MQ=60)
         CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     >  SRR4302541.5462387/1‑94 (MQ=60)
         CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     >  SRR4302541.2732593/1‑94 (MQ=60)
          GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    <  SRR4302541.7015996/94‑1 (MQ=60)
          GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    >  SRR4302541.7393134/1‑94 (MQ=60)
          GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    <  SRR4302541.7015995/94‑1 (MQ=60)
           ATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTT                                                                                   >  SRR4302541.490164/1‑94 (MQ=60)
           ATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTT                                                                                   >  SRR4302541.7278126/1‑94 (MQ=60)
           ATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTT                                                                                   <  SRR4302541.7516051/94‑1 (MQ=60)
           ATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTT                                                                                   >  SRR4302541.6503302/1‑94 (MQ=60)
             TAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTTGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCA                                                                                 <  SRR4302541.3079466/94‑1 (MQ=60)
             TAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCA                                                                                 <  SRR4302541.7221187/94‑1 (MQ=60)
             TAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCA                                                                                 >  SRR4302541.273697/1‑94 (MQ=60)
              AAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAA                                                                                >  SRR4302541.7109976/1‑94 (MQ=60)
               AAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAC                                                                               >  SRR4302541.5402712/1‑94 (MQ=60)
               AAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAC                                                                               >  SRR4302541.4404400/1‑94 (MQ=60)
               AAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAC                                                                               >  SRR4302541.1477911/1‑94 (MQ=60)
                AGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACT                                                                              >  SRR4302541.6247428/1‑94 (MQ=60)
                AGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACT                                                                              <  SRR4302541.6075220/94‑1 (MQ=60)
                AGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTAAACT                                                                              >  SRR4302541.7475086/1‑94 (MQ=60)
                  GTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCT                                                                            >  SRR4302541.6324256/1‑94 (MQ=60)
                  GTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCT                                                                            <  SRR4302541.5757799/94‑1 (MQ=60)
                   tgtcctctgcGGCGGTCACCATATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           <  SRR4302541.6322625/84‑1 (MQ=60)
                   TGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           >  SRR4302541.5888358/1‑94 (MQ=60)
                   TGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           >  SRR4302541.5888357/1‑94 (MQ=60)
                    GGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCA                                                                          >  SRR4302541.689154/1‑94 (MQ=60)
                      CCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACT                                                                        <  SRR4302541.4723680/94‑1 (MQ=60)
                      CCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACT                                                                        >  SRR4302541.485480/1‑94 (MQ=60)
                       CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       >  SRR4302541.6551870/1‑94 (MQ=60)
                       CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       >  SRR4302541.7821315/1‑94 (MQ=60)
                          TCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCA                                                                    <  SRR4302541.8838933/94‑1 (MQ=60)
                           CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   >  SRR4302541.6044334/1‑94 (MQ=60)
                           CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   >  SRR4302541.6086773/1‑94 (MQ=60)
                           CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   <  SRR4302541.7090344/94‑1 (MQ=60)
                           CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   >  SRR4302541.2689847/1‑94 (MQ=60)
                            AGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGT                                                                  <  SRR4302541.3330781/94‑1 (MQ=60)
                            AGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGT                                                                  >  SRR4302541.2086249/1‑94 (MQ=60)
                             GGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTA                                                                 >  SRR4302541.2373512/1‑94 (MQ=60)
                             GGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTA                                                                 <  SRR4302541.6977320/94‑1 (MQ=60)
                              GCGGTCACCGTATTCGATAATAACTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCCACTCTCACTACCAGTAG                                                                >  SRR4302541.5227308/1‑94 (MQ=60)
                              GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302541.8788811/94‑1 (MQ=60)
                              GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                >  SRR4302541.5227309/1‑94 (MQ=60)
                              GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302541.5358726/94‑1 (MQ=60)
                               CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               <  SRR4302541.4130419/94‑1 (MQ=60)
                               CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               <  SRR4302541.3388724/94‑1 (MQ=60)
                               CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               <  SRR4302541.911066/94‑1 (MQ=60)
                                GGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGA                                                              <  SRR4302541.1558877/94‑1 (MQ=60)
                                   CACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCC                                                           >  SRR4302541.1934864/1‑94 (MQ=60)
                                    ACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          >  SRR4302541.6733070/1‑94 (MQ=60)
                                      CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCTCAA                                                        >  SRR4302541.8213954/1‑94 (MQ=60)
                                      CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302541.1449854/1‑94 (MQ=60)
                                      CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302541.4629238/1‑94 (MQ=60)
                                      CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302541.2834806/1‑94 (MQ=60)
                                      CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        >  SRR4302541.6532576/1‑94 (MQ=60)
                                       GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       >  SRR4302541.6585925/1‑94 (MQ=60)
                                       GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       <  SRR4302541.1755043/94‑1 (MQ=60)
                                       GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       >  SRR4302541.3065832/1‑94 (MQ=60)
                                        TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCTGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      >  SRR4302541.2821859/1‑94 (MQ=60)
                                         ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     >  SRR4302541.8393038/1‑94 (MQ=60)
                                         ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     <  SRR4302541.1347336/94‑1 (MQ=60)
                                         ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     <  SRR4302541.5701517/94‑1 (MQ=60)
                                           TCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCG                                                   >  SRR4302541.5175831/1‑94 (MQ=60)
                                            CGATAATAAATGGGTCTTGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGA                                                  >  SRR4302541.3666825/1‑94 (MQ=60)
                                            CGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTTGA                                                  >  SRR4302541.7825295/1‑94 (MQ=60)
                                            CGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGA                                                  >  SRR4302541.7755209/1‑94 (MQ=60)
                                            CGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGA                                                  >  SRR4302541.1020435/1‑94 (MQ=60)
                                             GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302541.8383555/94‑1 (MQ=60)
                                             GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302541.3491418/94‑1 (MQ=60)
                                             GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302541.3491420/94‑1 (MQ=60)
                                             GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCCGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302541.1274015/94‑1 (MQ=60)
                                              ATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGC                                                >  SRR4302541.8235328/1‑94 (MQ=60)
                                               TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCACGTTCGCGCG                                               >  SRR4302541.3161742/1‑94 (MQ=60)
                                               TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               <  SRR4302541.4677734/94‑1 (MQ=60)
                                               TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302541.3161740/1‑94 (MQ=60)
                                               TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302541.4511919/1‑94 (MQ=60)
                                               TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302541.1454521/1‑94 (MQ=60)
                                                 ATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCG                                             >  SRR4302541.2299039/1‑94 (MQ=60)
                                                 ATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCG                                             >  SRR4302541.6902045/1‑94 (MQ=60)
                                                  TAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGG                                            <  SRR4302541.6694688/94‑1 (MQ=60)
                                                  TAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGG                                            <  SRR4302541.2000019/94‑1 (MQ=60)
                                                   AAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCTGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGA                                           <  SRR4302541.2638928/94‑1 (MQ=60)
                                                     ATGGGTCTGGAAAAAGTGCAGCATCTGGCATCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAT                                         <  SRR4302541.1014719/94‑1 (MQ=60)
                                                     ATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAT                                         <  SRR4302541.6936604/94‑1 (MQ=60)
                                                      TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCTAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        >  SRR4302541.7534746/1‑94 (MQ=60)
                                                       GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       <  SRR4302541.5397486/94‑1 (MQ=60)
                                                       GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       <  SRR4302541.6621698/94‑1 (MQ=60)
                                                       GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       <  SRR4302541.8711842/94‑1 (MQ=60)
                                                       GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       >  SRR4302541.4733180/1‑94 (MQ=60)
                                                        GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      >  SRR4302541.1883005/1‑94 (MQ=60)
                                                        GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      >  SRR4302541.5963787/1‑94 (MQ=60)
                                                        GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      <  SRR4302541.5586419/94‑1 (MQ=60)
                                                        GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      >  SRR4302541.4567055/1‑94 (MQ=60)
                                                        GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCCCGGAATGTA                                      >  SRR4302541.3941460/1‑94 (MQ=60)
                                                         GTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAT                                     <  SRR4302541.5514853/94‑1 (MQ=60)
                                                         GTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAT                                     <  SRR4302541.2891241/94‑1 (MQ=60)
                                                           CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302541.7156567/1‑94 (MQ=60)
                                                           CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302541.4046138/94‑1 (MQ=60)
                                                           CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302541.2284252/94‑1 (MQ=60)
                                                           CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302541.857104/94‑1 (MQ=60)
                                                           CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302541.1612536/94‑1 (MQ=60)
                                                            TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302541.8597339/94‑1 (MQ=60)
                                                            TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302541.3630512/94‑1 (MQ=60)
                                                            TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGGATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  >  SRR4302541.8849674/1‑94 (MQ=60)
                                                             GGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGT                                 >  SRR4302541.4826157/1‑94 (MQ=60)
                                                             GGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGT                                 <  SRR4302541.2716746/94‑1 (MQ=60)
                                                              GAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTA                                >  SRR4302541.4703299/1‑94 (MQ=60)
                                                              GAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTA                                >  SRR4302541.8175511/1‑94 (MQ=60)
                                                              GAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTA                                >  SRR4302541.2632090/1‑94 (MQ=60)
                                                               AAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCATGTTCGAGCGCGGAATGTATCTGTAT                               <  SRR4302541.5112236/94‑1 (MQ=60)
                                                               AAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAT                               <  SRR4302541.4653980/94‑1 (MQ=60)
                                                               AAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCACAAGTTCGAGCGCGGAATGTATCTGTAT                               <  SRR4302541.2476743/94‑1 (MQ=60)
                                                                AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302541.8267545/94‑1 (MQ=60)
                                                                AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302541.5049245/94‑1 (MQ=60)
                                                                AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302541.3248345/94‑1 (MQ=60)
                                                                AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302541.3406810/94‑1 (MQ=60)
                                                                AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              >  SRR4302541.3472872/1‑94 (MQ=60)
                                                                 AAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGA                             <  SRR4302541.5821502/94‑1 (MQ=60)
                                                                 AAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGA                             >  SRR4302541.4696605/1‑94 (MQ=60)
                                                                 AAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGA                             >  SRR4302541.4151361/1‑94 (MQ=60)
                                                                  AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302541.8798251/94‑1 (MQ=60)
                                                                  AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302541.1093741/94‑1 (MQ=60)
                                                                  AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            >  SRR4302541.292587/1‑94 (MQ=60)
                                                                  AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            >  SRR4302541.2943862/1‑94 (MQ=60)
                                                                   AGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           <  SRR4302541.7674940/94‑1 (MQ=60)
                                                                   AGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           >  SRR4302541.4913004/1‑94 (MQ=60)
                                                                   AGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATTCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           >  SRR4302541.8067025/1‑94 (MQ=60)
                                                                     TGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAT                         >  SRR4302541.119423/1‑94 (MQ=60)
                                                                     TGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAC                         >  SRR4302541.6590781/1‑94 (MQ=60)
                                                                     TGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTNCAACTCTCACTACCAGTATGATCCCAAGTTCGNGCNNNGANTGTATCTNNANNNGNAT                         >  SRR4302541.468595/1‑94 (MQ=60)
                                                                      GCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATC                        <  SRR4302541.1642998/94‑1 (MQ=60)
                                                                      GCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATC                        >  SRR4302541.4887900/1‑94 (MQ=60)
                                                                      GCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATC                        <  SRR4302541.8691211/94‑1 (MQ=60)
                                                                       CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       <  SRR4302541.4694552/94‑1 (MQ=60)
                                                                       CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       <  SRR4302541.4043866/94‑1 (MQ=60)
                                                                        AGCATCTGGCAGTGTAATGCGGGTAGCCCAGTTCAACTCTAACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      >  SRR4302541.3030911/1‑94 (MQ=60)
                                                                        AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGActgaatctgaatgacaatcgc                      >  SRR4302541.2744936/1‑73 (MQ=60)
                                                                        AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      <  SRR4302541.8211363/94‑1 (MQ=60)
                                                                        AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      >  SRR4302541.2744937/1‑94 (MQ=60)
                                                                         GCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTC                     <  SRR4302541.209532/94‑1 (MQ=60)
                                                                         GCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTC                     <  SRR4302541.7318370/94‑1 (MQ=60)
                                                                          CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    >  SRR4302541.1014989/1‑94 (MQ=60)
                                                                          CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    >  SRR4302541.2414111/1‑94 (MQ=60)
                                                                          CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    <  SRR4302541.195716/94‑1 (MQ=60)
                                                                           ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCGCTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   <  SRR4302541.3515151/94‑1 (MQ=60)
                                                                           ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302541.3201687/1‑94 (MQ=60)
                                                                           ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302541.7803812/1‑94 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302541.4857325/94‑1 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302541.2193722/94‑1 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302541.8619973/94‑1 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  >  SRR4302541.4843955/1‑94 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302541.2676148/94‑1 (MQ=60)
                                                                            TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302541.2734127/94‑1 (MQ=60)
                                                                             CTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTG                 <  SRR4302541.118493/94‑1 (MQ=60)
                                                                               GGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTT               <  SRR4302541.2670213/94‑1 (MQ=60)
                                                                               GGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTT               >  SRR4302541.4491177/1‑94 (MQ=60)
                                                                               GGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTT               <  SRR4302541.220660/94‑1 (MQ=60)
                                                                                  AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302541.58451/1‑94 (MQ=60)
                                                                                   GCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAA           <  SRR4302541.6845073/94‑1 (MQ=60)
                                                                                    CGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAAT          <  SRR4302541.2701651/94‑1 (MQ=60)
                                                                                    CGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAAT          >  SRR4302541.5050979/1‑94 (MQ=60)
                                                                                     GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         >  SRR4302541.5419712/1‑94 (MQ=60)
                                                                                      TAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAA        >  SRR4302541.4983679/1‑94 (MQ=60)
                                                                                       AATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATCAC       >  SRR4302541.8606762/1‑94 (MQ=60)
                                                                                        ATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA      >  SRR4302541.6926733/1‑94 (MQ=60)
                                                                                        ATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA      <  SRR4302541.8812707/94‑1 (MQ=60)
                                                                                         TGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACAT     <  SRR4302541.4415936/94‑1 (MQ=60)
                                                                                         TGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACAT     <  SRR4302541.534874/94‑1 (MQ=60)
                                                                                         TGCGGGTAGCCCAGTTCAACTATCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACAT     >  SRR4302541.6960612/1‑94 (MQ=60)
                                                                                           CGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA   <  SRR4302541.1696941/94‑1 (MQ=60)
                                                                                            GGGTAGCCCAGTTCAACTCTCACTTCCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  <  SRR4302541.5544272/94‑1 (MQ=60)
                                                                                            GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  <  SRR4302541.4664947/94‑1 (MQ=60)
                                                                                            GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  <  SRR4302541.7479025/94‑1 (MQ=60)
                                                                                            GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  SRR4302541.3246295/1‑94 (MQ=60)
                                                                                            GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAACATCGTCGCTGTTTCAATAACATCAT  >  SRR4302541.5549729/1‑94 (MQ=60)
                                                                                            |                                                                                             
AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532319‑4532504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑