Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,411	A→G	intergenic (‑1059/‑172)	yjhQ ← / → yjhR	hypothetical protein/putative frameshift suppressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,411	0	A	G	100.0%	821.8 / NA	236	intergenic (‑1059/‑172)	yjhQ/yjhR	hypothetical protein/putative frameshift suppressor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (124/112); total (124/112)

Only 100 of 242 total aligned reads displayed.

CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA  >  NC_000913/4532321‑4532500

cTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCggg                                                                                         >  2:445483/1‑93 (MQ=255)

 tGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGt                                                                                        >  2:6287464/1‑93 (MQ=255)

 tGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGt                                                                                        <  2:2232708/93‑1 (MQ=255)

  gCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTa                                                                                       >  2:5003646/1‑93 (MQ=255)

  gCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTa                                                                                       <  1:3532801/93‑1 (MQ=255)

     cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  1:6209360/1‑93 (MQ=255)

     cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    <  1:2154273/93‑1 (MQ=255)

     cACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGcc                                                                                    >  1:5173865/1‑93 (MQ=255)

      aCGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGacc                                                                                   >  1:250609/1‑93 (MQ=255)

       cGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCa                                                                                  >  1:4805777/1‑93 (MQ=255)

          ttAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGtt                                                                               <  2:4442043/93‑1 (MQ=255)

             aaGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCaa                                                                            >  2:1405170/1‑93 (MQ=255)

             aaGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCaa                                                                            <  1:4323516/93‑1 (MQ=255)

              aGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGGTCAAc                                                                           >  2:1912372/1‑93 (MQ=255)

                 tGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAActct                                                                        <  1:2775023/93‑1 (MQ=255)

                   gCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCa                                                                      <  1:1645839/93‑1 (MQ=255)

                    ccGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCAc                                                                     <  2:5244493/93‑1 (MQ=255)

                      gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTa                                                                   >  2:829943/1‑93 (MQ=255)

                      gCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTa                                                                   >  2:4269466/1‑93 (MQ=255)

                       cTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAc                                                                  >  1:2870855/1‑93 (MQ=255)

                        tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAcc                                                                 >  1:1979943/1‑93 (MQ=255)

                        tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAcc                                                                 <  1:1569566/93‑1 (MQ=255)

                        tCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAcc                                                                 <  1:4255374/93‑1 (MQ=255)

                           ggCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGt                                                              >  1:1095293/1‑93 (MQ=255)

                            gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                             <  1:2359050/93‑1 (MQ=255)

                            gCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTa                                                             <  1:1384566/93‑1 (MQ=255)

                               gTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGa                                                          <  1:369819/93‑1 (MQ=255)

                               gTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGa                                                          <  2:2421192/93‑1 (MQ=255)

                                 cACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATc                                                        >  2:1258313/1‑93 (MQ=255)

                                  aCCGTATTCGGTAATAAATGGGACTAGAAAAACGGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCACTATGAtgc                                                       >  1:6250854/1‑91 (MQ=255)

                                   ccGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGTGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                      <  2:1289393/93‑1 (MQ=255)

                                   ccGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATccc                                                      >  1:14432/1‑93 (MQ=255)

                                    cGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCa                                                     <  1:3921990/93‑1 (MQ=255)

                                    cGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCa                                                     >  2:5659312/1‑93 (MQ=255)

                                      tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                   <  1:4094087/93‑1 (MQ=255)

                                      tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                   >  2:1183750/1‑93 (MQ=255)

                                      tATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAg                                                   >  2:3405821/1‑93 (MQ=255)

                                       aTTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGt                                                  <  1:4477872/93‑1 (MQ=255)

                                       aTTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGt                                                  <  1:1590928/93‑1 (MQ=255)

                                        ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                 >  1:2477074/1‑93 (MQ=255)

                                        ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                 <  1:5295579/93‑1 (MQ=255)

                                        ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                 <  2:668117/93‑1 (MQ=255)

                                        ttCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGtt                                                 <  1:1115068/93‑1 (MQ=255)

                                          cGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCg                                               <  2:4826370/93‑1 (MQ=255)

                                           gATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGCAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGa                                              >  2:1617419/1‑93 (MQ=255)

                                             taataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgc                                            <  2:3600390/93‑1 (MQ=255)

                                               ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                          >  1:557537/1‑93 (MQ=255)

                                               ataaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAgcgc                                          >  1:5194777/1‑93 (MQ=255)

                                                taaATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGcgcg                                         >  2:469643/1‑93 (MQ=255)

                                                 aaaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCgg                                        >  1:586766/1‑93 (MQ=255)

                                                 aaaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCgg                                        >  2:557097/1‑93 (MQ=255)

                                                  aaTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGa                                       >  1:475477/1‑93 (MQ=255)

                                                   aTGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGaa                                      <  2:2650018/93‑1 (MQ=255)

                                                     gggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATg                                    >  2:6133658/1‑93 (MQ=255)

                                                     gggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATg                                    >  1:3498899/1‑93 (MQ=255)

                                                      ggTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGt                                   <  1:206115/93‑1 (MQ=255)

                                                        tCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTAt                                 <  2:4675183/93‑1 (MQ=255)

                                                         cTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATc                                <  1:6175679/93‑1 (MQ=255)

                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               >  1:5418851/1‑93 (MQ=255)

                                                          tGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCt                               <  1:3957837/93‑1 (MQ=255)

                                                          cggAAAAAGTGCAGCATCTGGCAGCGTAATGCGGCTAGCCCAGTTCAACTCTCACTACCAGTATGAACCCAAGTTCGAGCGCGGAATGTATCt                               <  2:3937788/92‑1 (MQ=255)

                                                           tgAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTg                              <  2:3823942/92‑1 (MQ=255)

                                                            gAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGt                             <  2:2475557/93‑1 (MQ=255)

                                                             aaaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCACAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTa                            >  1:3347023/1‑93 (MQ=255)

                                                              aaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAt                           <  2:2005555/93‑1 (MQ=255)

                                                              aaaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTAt                           <  1:5159535/93‑1 (MQ=255)

                                                               aaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATg                          <  2:2486797/93‑1 (MQ=255)

                                                               aaaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATg                          >  2:3452655/1‑93 (MQ=255)

                                                                aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                         <  1:2990027/93‑1 (MQ=255)

                                                                aaGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGa                         >  1:494629/1‑93 (MQ=255)

                                                                 aGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAg                        <  2:3174339/93‑1 (MQ=255)

                                                                  gTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGc                       <  2:5904897/93‑1 (MQ=255)

                                                                   tGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATGCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCa                      >  2:5618089/1‑93 (MQ=255)

                                                                    gcagcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAt                     >  1:669708/1‑93 (MQ=255)

                                                                      agcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcg                   >  2:4603181/1‑93 (MQ=255)

                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGCGAGCGGAATGCATCTGTATGAGCAtcgt                  >  2:2586900/1‑93 (MQ=255)

                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  <  2:2131418/93‑1 (MQ=255)

                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  >  2:3546114/1‑93 (MQ=255)

                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  <  2:1568662/93‑1 (MQ=255)

                                                                       gcaTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgt                  >  1:3626484/1‑93 (MQ=255)

                                                                        caTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtc                 <  1:3106291/93‑1 (MQ=255)

                                                                         aTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                <  1:6359513/93‑1 (MQ=255)

                                                                         aTCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAtcgtcg                >  1:4631237/1‑93 (MQ=255)

                                                                          tCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGc               <  1:404083/93‑1 (MQ=255)

                                                                          tCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGc               >  1:5939732/1‑93 (MQ=255)

                                                                            tGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTg             >  2:6421877/1‑93 (MQ=255)

                                                                             ggCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGt            >  2:4100397/1‑93 (MQ=255)

                                                                             ggCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGt            >  1:4206156/1‑93 (MQ=255)

                                                                             ggCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGt            >  1:980334/1‑93 (MQ=255)

                                                                              gCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGtt           <  1:2238711/93‑1 (MQ=255)

                                                                              gCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGtt           <  1:1033960/93‑1 (MQ=255)

                                                                                aGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTc         >  1:710397/1‑93 (MQ=255)

                                                                                aGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATAGTCGCTGTTTc         >  2:4496317/1‑93 (MQ=255)

                                                                                 gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa        >  1:5915721/1‑93 (MQ=255)

                                                                                 gCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCa        >  1:565136/1‑93 (MQ=255)

                                                                                    tAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCGTCGTCGCTGTTTCAATa     <  2:5051190/93‑1 (MQ=255)

                                                                                    tAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATa     >  2:4541333/1‑93 (MQ=255)

                                                                                     taTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATaa    <  2:3904929/92‑1 (MQ=255)

                                                                                      aTGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAc   >  2:1045917/1‑93 (MQ=255)

                                                                                       tGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAca  <  2:5482008/93‑1 (MQ=255)

CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACA  >  NC_000913/4532321‑4532500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532318‑4532504

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCG                                                                                               >  SRR4302534.3490059/1‑94 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              <  SRR4302534.6142117/94‑1 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              <  SRR4302534.5052082/94‑1 (MQ=60)

 AACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGG                                                                                              >  SRR4302534.4191203/1‑94 (MQ=60)

  ACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGG                                                                                             >  SRR4302534.6258647/1‑94 (MQ=60)

   CTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGT                                                                                            <  SRR4302534.1436009/94‑1 (MQ=60)

     GCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAG                                                                                          <  SRR4302534.2641227/94‑1 (MQ=60)

     GCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAG                                                                                          <  SRR4302534.3627830/94‑1 (MQ=60)

      CCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGC                                                                                         >  SRR4302534.500042/1‑94 (MQ=60)

       CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302534.6378145/1‑94 (MQ=60)

       CCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCC                                                                                        >  SRR4302534.5313297/1‑94 (MQ=60)

        CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       <  SRR4302534.2211141/94‑1 (MQ=60)

        CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCC                                                                                       >  SRR4302534.5339660/1‑94 (MQ=60)

        CACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGACC                                                                                       >  SRR4302534.257146/1‑94 (MQ=60)

         ACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCA                                                                                      >  SRR4302534.1016388/1‑94 (MQ=60)

         ACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCA                                                                                      >  SRR4302534.2983068/1‑94 (MQ=60)

         ACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCA                                                                                      >  SRR4302534.4936255/1‑94 (MQ=60)

          CGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAG                                                                                     <  SRR4302534.716837/94‑1 (MQ=60)

           GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    >  SRR4302534.681100/1‑94 (MQ=60)

           GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    >  SRR4302534.2987871/1‑94 (MQ=60)

           GATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGT                                                                                    >  SRR4302534.3468376/1‑94 (MQ=60)

             TTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTC                                                                                  <  SRR4302534.2474847/94‑1 (MQ=60)

             TTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTC                                                                                  <  SRR4302534.6064089/94‑1 (MQ=60)

               AAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAA                                                                                >  SRR4302534.6359352/1‑94 (MQ=60)

                AAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAC                                                                               <  SRR4302534.4440512/94‑1 (MQ=60)

                AAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAAC                                                                               <  SRR4302534.3364766/94‑1 (MQ=60)

                   GTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCT                                                                            <  SRR4302534.4519598/94‑1 (MQ=60)

                    TGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           <  SRR4302534.3588301/94‑1 (MQ=60)

                    TGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           <  SRR4302534.2848041/94‑1 (MQ=60)

                    GGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTC                                                                           <  SRR4302534.4389014/94‑1 (MQ=60)

                      GCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCAC                                                                         <  SRR4302534.1688737/94‑1 (MQ=60)

                       CCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACT                                                                        >  SRR4302534.232539/1‑94 (MQ=60)

                       CCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACT                                                                        <  SRR4302534.4908333/94‑1 (MQ=60)

                        CGCTCAGGCGGTCACCGTATTCGATAATCAATGGGCCTGGAAAAATCGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       >  SRR4302534.5157503/1‑94 (MQ=60)

                        CGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTA                                                                       <  SRR4302534.4155253/94‑1 (MQ=60)

                         GCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTAC                                                                      >  SRR4302534.2946236/1‑94 (MQ=60)

                          CTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACC                                                                     >  SRR4302534.2031787/1‑94 (MQ=60)

                           TCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCA                                                                    <  SRR4302534.4370226/94‑1 (MQ=60)

                           TCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCA                                                                    <  SRR4302534.1610670/94‑1 (MQ=60)

                            CAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAG                                                                   <  SRR4302534.946852/94‑1 (MQ=60)

                             AGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGT                                                                  >  SRR4302534.1123783/1‑94 (MQ=60)

                              GGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTA                                                                 >  SRR4302534.3632314/1‑94 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302534.1421021/94‑1 (MQ=60)

                               GCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTAT                                                                <  SRR4302534.2421671/94‑1 (MQ=60)

                                CTGTCACCGTATTCGATAATAAATGGGACTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATG                                                               >  SRR4302534.2060022/1‑94 (MQ=60)

                                CGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTTAACTCTCACTACCAGTATG                                                               >  SRR4302534.1851907/1‑94 (MQ=60)

                                 GGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGA                                                              >  SRR4302534.4281298/1‑94 (MQ=60)

                                 GGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGA                                                              >  SRR4302534.3627650/1‑94 (MQ=60)

                                  GTCTCCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGAT                                                             <  SRR4302534.4887312/94‑1 (MQ=60)

                                  GTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGAT                                                             <  SRR4302534.379245/94‑1 (MQ=60)

                                   TCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATC                                                            >  SRR4302534.6308643/1‑94 (MQ=60)

                                    CACCGTATTCGGTAATAAATGGGACTAGAAAAACGGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCACTATGATGC                                                           >  SRR4302534.6420341/1‑94 (MQ=60)

                                     CCCGTCTTCGATAATAAATGGGACTGGAAAAAGTGCAGCATCTGGCAGCGTCATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          >  SRR4302534.2816902/1‑94 (MQ=60)

                                     ACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          >  SRR4302534.14816/1‑94 (MQ=60)

                                     ACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCC                                                          <  SRR4302534.5571932/94‑1 (MQ=60)

                                       CGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAA                                                        <  SRR4302534.4027079/94‑1 (MQ=60)

                                        GTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAG                                                       >  SRR4302534.6093418/1‑94 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      >  SRR4302534.2163905/1‑94 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      >  SRR4302534.3702196/1‑94 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      <  SRR4302534.4204107/94‑1 (MQ=60)

                                         TATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGT                                                      <  SRR4302534.4020138/94‑1 (MQ=60)

                                          ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     <  SRR4302534.4599013/94‑1 (MQ=60)

                                          ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     <  SRR4302534.1632536/94‑1 (MQ=60)

                                          ATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTT                                                     >  SRR4302534.2542900/1‑94 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    <  SRR4302534.5438504/94‑1 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    <  SRR4302534.3875757/94‑1 (MQ=60)

                                           TTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTC                                                    <  SRR4302534.1144179/94‑1 (MQ=60)

                                            TCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCG                                                   <  SRR4302534.2123092/94‑1 (MQ=60)

                                              GATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAG                                                 <  SRR4302534.4046613/94‑1 (MQ=60)

                                               ATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGC                                                <  SRR4302534.1798456/94‑1 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302534.2237744/1‑94 (MQ=60)

                                                TAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCG                                               >  SRR4302534.636133/1‑94 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302534.571282/1‑94 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302534.4811603/1‑94 (MQ=60)

                                                 AATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGC                                              >  SRR4302534.5334862/1‑94 (MQ=60)

                                                   TAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGG                                            >  SRR4302534.601097/1‑94 (MQ=60)

                                                    AAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGA                                           >  SRR4302534.487439/1‑94 (MQ=60)

                                                     AATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAA                                          <  SRR4302534.3078953/94‑1 (MQ=60)

                                                      ATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAAT                                         <  SRR4302534.3757487/94‑1 (MQ=60)

                                                       TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        >  SRR4302534.3592993/1‑94 (MQ=60)

                                                       TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        <  SRR4302534.5274933/94‑1 (MQ=60)

                                                       TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        <  SRR4302534.1594855/94‑1 (MQ=60)

                                                       TGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATG                                        <  SRR4302534.4326116/94‑1 (MQ=60)

                                                        GGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGT                                       <  SRR4302534.6398851/94‑1 (MQ=60)

                                                         GGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTA                                      <  SRR4302534.211521/94‑1 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302534.6343720/94‑1 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   >  SRR4302534.5564656/1‑94 (MQ=60)

                                                            CTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCT                                   <  SRR4302534.4127693/94‑1 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302534.4063814/94‑1 (MQ=60)

                                                             TGGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTG                                  <  SRR4302534.4701649/94‑1 (MQ=60)

                                                              GGAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGT                                 <  SRR4302534.672087/94‑1 (MQ=60)

                                                               GAAAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCACAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTA                                >  SRR4302534.3436628/1‑94 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302534.1549404/94‑1 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              >  SRR4302534.6504811/1‑94 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              <  SRR4302534.5298655/94‑1 (MQ=60)

                                                                 AAAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATG                              >  SRR4302534.5309386/1‑94 (MQ=60)

                                                                  AAAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGA                             >  SRR4302534.506971/1‑94 (MQ=60)

                                                                   AAGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302534.3068197/94‑1 (MQ=60)

                                                                   AAGTGCAGCAGCTGCCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAG                            <  SRR4302534.1164029/94‑1 (MQ=60)

                                                                    AGTGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGC                           >  SRR4302534.4356326/1‑94 (MQ=60)

                                                                      TGCAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCAT                         >  SRR4302534.686028/1‑94 (MQ=60)

                                                                        CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       <  SRR4302534.1065311/94‑1 (MQ=60)

                                                                        CAGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCG                       >  SRR4302534.1159146/1‑94 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      <  SRR4302534.1686403/94‑1 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      >  SRR4302534.3723963/1‑94 (MQ=60)

                                                                         AGCATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGT                      <  SRR4302534.530603/94‑1 (MQ=60)

                                                                           CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    >  SRR4302534.5394396/1‑94 (MQ=60)

                                                                           CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    <  SRR4302534.3187983/94‑1 (MQ=60)

                                                                           CATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCG                    >  SRR4302534.4756907/1‑94 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   <  SRR4302534.6531261/94‑1 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302534.6101012/1‑94 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302534.1617592/1‑94 (MQ=60)

                                                                            ATCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGC                   >  SRR4302534.5977937/1‑94 (MQ=60)

                                                                             TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  >  SRR4302534.5561694/1‑94 (MQ=60)

                                                                             TCTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCT                  <  SRR4302534.414409/94‑1 (MQ=60)

                                                                              CTGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTG                 <  SRR4302534.241173/94‑1 (MQ=60)

                                                                               TGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGT                >  SRR4302534.1005351/1‑94 (MQ=60)

                                                                               TGGCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGT                >  SRR4302534.4319687/1‑94 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              <  SRR4302534.2298037/94‑1 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              >  SRR4302534.5976066/1‑94 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              <  SRR4302534.1060637/94‑1 (MQ=60)

                                                                                 GCAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTT              >  SRR4302534.5733630/1‑94 (MQ=60)

                                                                                  CAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTGCCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTC             <  SRR4302534.4155819/94‑1 (MQ=60)

                                                                                  CAGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTC             >  SRR4302534.727843/1‑94 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            <  SRR4302534.2910048/94‑1 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302534.579031/1‑94 (MQ=60)

                                                                                   AGCGTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCA            >  SRR4302534.6076359/1‑94 (MQ=60)

                                                                                      GTAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATA         <  SRR4302534.5702280/94‑1 (MQ=60)

                                                                                       TAATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAA        >  SRR4302534.1143177/1‑94 (MQ=60)

                                                                                        AATGCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAC       <  SRR4302534.4453863/94‑1 (MQ=60)

                                                                                        AATGCGGGTAGCACAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAAC       >  SRR4302534.2545080/1‑94 (MQ=60)

                                                                                           GCGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATC    <  SRR4302534.804012/94‑1 (MQ=60)

                                                                                            CGGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCA   <  SRR4302534.4530841/94‑1 (MQ=60)

                                                                                             GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  <  SRR4302534.4233580/94‑1 (MQ=60)

                                                                                             GGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  <  SRR4302534.240236/94‑1 (MQ=60)

TAACTGCCCACGATTAAAGGTGGCCGCTCAGGCGGTCACCGTATTCGATAATAAATGGGTCTGGAAAAAGTGCAGCATCTGGCAGCGTAATGCAGGTAGCCCAGTTCAACTCTCACTACCAGTATGATCCCAAGTTCGAGCGCGGAATGTATCTGTATGAGCATCGTCGCTGTTTCAATAACATCAT  >  NC_000913/4532318‑4532504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`