Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,269,236 G→C A59G (GCT→GGT)  b3124 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,2360GC100.0% 684.6 / NA 196A59G (GCT→GGT) b3124hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (99/97);  total (99/97)

Only 100 of 204 total aligned reads displayed.

CAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATT  >  NC_000913/3269147‑3269325
                                                                                         |                                                                                         
 aaTAAACGCGGGTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACcccc                                                                                       <  2:1116107/93‑1 (MQ=255)
   tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCt                                                                                     <  1:121887/93‑1 (MQ=255)
   tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCt                                                                                     <  1:1545935/93‑1 (MQ=255)
     aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTgg                                                                                   >  2:2147580/1‑93 (MQ=255)
      aCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTggg                                                                                  >  2:897551/1‑93 (MQ=255)
      aCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTggg                                                                                  >  2:3847839/1‑93 (MQ=255)
       ggcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGt                                                                                 <  1:749221/92‑1 (MQ=255)
        gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTg                                                                                >  1:2199679/1‑93 (MQ=255)
          ggTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGc                                                                              <  1:5860109/93‑1 (MQ=255)
           gTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCt                                                                             <  2:4843503/93‑1 (MQ=255)
            ttttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTg                                                                            >  2:1207581/1‑93 (MQ=255)
             tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGTCGTGACGTTCACCCCCCTGGGTGGCTGc                                                                           <  1:4185256/93‑1 (MQ=255)
             tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGc                                                                           >  1:2891007/1‑93 (MQ=255)
                gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAat                                                                        >  1:857273/1‑93 (MQ=255)
                 ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatc                                                                       <  1:941544/93‑1 (MQ=255)
                 ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatc                                                                       >  2:3581645/1‑93 (MQ=255)
                 ccATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatc                                                                       >  2:6095896/1‑93 (MQ=255)
                   ctCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatcat                                                                     <  1:1615869/92‑1 (MQ=255)
                    tCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatcatc                                                                    >  2:6345148/1‑93 (MQ=255)
                    tCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAatcatc                                                                    <  1:2745851/93‑1 (MQ=255)
                      gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGc                                                                  >  1:1027769/1‑93 (MQ=255)
                      gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGc                                                                  <  2:2060184/93‑1 (MQ=255)
                       ccGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCt                                                                 <  2:596660/93‑1 (MQ=255)
                       ccGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCt                                                                 <  2:5595475/93‑1 (MQ=255)
                           agaTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCtgtca                                                             >  1:5687478/1‑89 (MQ=255)
                           agaTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCa                                                             >  1:2791836/1‑93 (MQ=255)
                             aTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc                                                           >  2:3397525/1‑93 (MQ=255)
                             aTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc                                                           >  2:5757515/1‑93 (MQ=255)
                             aTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc                                                           <  1:194092/93‑1 (MQ=255)
                               cccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGt                                                         <  1:3688253/93‑1 (MQ=255)
                               cccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGt                                                         >  1:5788331/1‑93 (MQ=255)
                               cccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGt                                                         <  2:5118923/93‑1 (MQ=255)
                                ccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGtt                                                        >  2:5816963/1‑93 (MQ=255)
                                ccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGtt                                                        >  1:2449153/1‑93 (MQ=255)
                                ccccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGtt                                                        >  1:5044601/1‑93 (MQ=255)
                                  ccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTcc                                                      <  1:4679317/93‑1 (MQ=255)
                                   cAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACACAGGCGTGACGTTCGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCt                                                     <  2:4201831/93‑1 (MQ=255)
                                    aaCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCtt                                                    <  1:2500896/93‑1 (MQ=255)
                                    aaCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCtt                                                    >  2:4382754/1‑93 (MQ=255)
                                    aaCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCtt                                                    <  1:6013427/93‑1 (MQ=255)
                                     aCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTc                                                   >  2:6219747/1‑93 (MQ=255)
                                      cTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCg                                                  >  1:3861170/1‑93 (MQ=255)
                                      cTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCg                                                  <  1:6130507/93‑1 (MQ=255)
                                       tGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                 <  2:2995604/93‑1 (MQ=255)
                                       tGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                 >  2:4131829/1‑93 (MQ=255)
                                       tGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGc                                                 >  1:3394845/1‑93 (MQ=255)
                                        ggCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGcc                                                <  2:2846617/93‑1 (MQ=255)
                                        ggCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGcc                                                >  1:4348653/1‑93 (MQ=255)
                                         gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCa                                               <  1:4251637/93‑1 (MQ=255)
                                          cATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAc                                              <  2:5117603/93‑1 (MQ=255)
                                             tCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGt                                           <  2:1951386/93‑1 (MQ=255)
                                              cACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTc                                          <  2:4574304/93‑1 (MQ=255)
                                                cTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCgg                                        <  1:2564075/93‑1 (MQ=255)
                                                 tttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGc                                       >  2:1373045/1‑93 (MQ=255)
                                                 tttCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGc                                       <  1:352858/93‑1 (MQ=255)
                                                        cccAGCGCCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGa                                >  1:367694/1‑93 (MQ=255)
                                                         ccAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGaa                               >  1:2681664/1‑93 (MQ=255)
                                                          cAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAc                              <  2:4215554/93‑1 (MQ=255)
                                                           aGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACa                             <  1:1689678/93‑1 (MQ=255)
                                                            gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                            <  1:319809/93‑1 (MQ=255)
                                                            gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                            <  1:2837027/93‑1 (MQ=255)
                                                            gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                            >  1:6355023/1‑93 (MQ=255)
                                                            gCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAg                            <  1:4363843/93‑1 (MQ=255)
                                                             cGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGa                           <  1:1745682/93‑1 (MQ=255)
                                                              ggCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaa                          >  2:3002286/1‑93 (MQ=255)
                                                               gCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                         <  2:1063486/93‑1 (MQ=255)
                                                               gCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaaa                         >  2:4775853/1‑93 (MQ=255)
                                                                cccTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAc                        >  2:3300647/1‑93 (MQ=255)
                                                                  cTGTACCCCAGGCGTGCCGTTCTCCCCCCTGGGTGGCTGCAATCATCGCTTCCGCCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                      <  2:4703966/93‑1 (MQ=255)
                                                                  cTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGt                      >  1:6166068/1‑93 (MQ=255)
                                                                   tGTAACCCAGGCGTGACGTTCGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                     <  2:1436881/93‑1 (MQ=255)
                                                                   tGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                     <  2:3191830/93‑1 (MQ=255)
                                                                    gTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAc                    <  1:5997739/93‑1 (MQ=255)
                                                                    gTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTACCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAc                    <  2:3713528/93‑1 (MQ=255)
                                                                     tAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACt                   <  1:239292/93‑1 (MQ=255)
                                                                      agcccAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtg                  <  2:2277849/91‑1 (MQ=255)
                                                                      aaCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtg                  <  2:2711824/93‑1 (MQ=255)
                                                                      aaCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtg                  >  2:4413674/1‑93 (MQ=255)
                                                                       aCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgt                 >  1:1795365/1‑93 (MQ=255)
                                                                       aCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgt                 >  2:4438374/1‑93 (MQ=255)
                                                                       aCCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgt                 <  2:2771469/93‑1 (MQ=255)
                                                                        cccAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgtg                <  2:1725642/93‑1 (MQ=255)
                                                                        cccAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgtg                <  2:2332396/93‑1 (MQ=255)
                                                                        cccAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACtgtg                <  1:557523/93‑1 (MQ=255)
                                                                          cAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAGAGAAACGAACTGTGct              >  1:3365984/1‑92 (MQ=255)
                                                                          cAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCa              >  1:5739907/1‑93 (MQ=255)
                                                                           aGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAt             <  2:3067115/93‑1 (MQ=255)
                                                                           aGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAt             >  1:5253947/1‑93 (MQ=255)
                                                                            ggCGTGACGTTCACCCCCCTGGGTGTCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATc            <  1:1374939/93‑1 (MQ=255)
                                                                            ggCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATc            <  1:3763635/93‑1 (MQ=255)
                                                                            ggCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATc            >  1:3094129/1‑93 (MQ=255)
                                                                             gCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCa           >  2:2421360/1‑93 (MQ=255)
                                                                              cGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAg          >  2:2516052/1‑93 (MQ=255)
                                                                                tGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGa        <  1:328428/93‑1 (MQ=255)
                                                                                   cGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaa     >  2:283494/1‑93 (MQ=255)
                                                                                    gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa    >  1:4938743/1‑93 (MQ=255)
                                                                                    gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa    >  1:4565571/1‑93 (MQ=255)
                                                                                    gTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGaaaaa    >  1:3013523/1‑93 (MQ=255)
                                                                                     ttCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAt   >  1:4481158/1‑93 (MQ=255)
                                                                                      tCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGGCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAtt  <  2:1884879/93‑1 (MQ=255)
                                                                                         |                                                                                         
CAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATT  >  NC_000913/3269147‑3269325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  NC_000913/3269143‑3269329
                                                                                             |                                                                                             
ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAC                                                                                               <  SRR4302534.5748794/94‑1 (MQ=60)
 TTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACC                                                                                              <  SRR4302534.4764388/94‑1 (MQ=60)
  TTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCC                                                                                             <  SRR4302534.1834224/94‑1 (MQ=60)
   TCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCC                                                                                            >  SRR4302534.3313477/1‑94 (MQ=60)
       TAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTG                                                                                        <  SRR4302534.125117/94‑1 (MQ=60)
       TAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTG                                                                                        <  SRR4302534.1586450/94‑1 (MQ=60)
        AAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGG                                                                                       >  SRR4302534.5343039/1‑94 (MQ=60)
        AAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGAAACCCAGGCGTGACGTTCACCCCCCTGG                                                                                       <  SRR4302534.1604666/94‑1 (MQ=60)
         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      >  SRR4302534.1769963/1‑94 (MQ=60)
         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      >  SRR4302534.1797239/1‑94 (MQ=60)
         AACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTCCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGG                                                                                      <  SRR4302534.3925101/94‑1 (MQ=60)
          ACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGT                                                                                     >  SRR4302534.5854103/1‑94 (MQ=60)
          ACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGG                                                                                     >  SRR4302534.3849942/1‑94 (MQ=60)
           GGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTG                                                                                    <  SRR4302534.767785/94‑1 (MQ=60)
           CGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTG                                                                                    >  SRR4302534.2257886/1‑94 (MQ=60)
           CGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTG                                                                                    >  SRR4302534.1853355/1‑94 (MQ=60)
              GGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCT                                                                                 >  SRR4302534.6017950/1‑94 (MQ=60)
              GGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCT                                                                                 <  SRR4302534.6019219/94‑1 (MQ=60)
              GGTTTTGCCATCGCCGGAGA‑CCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTG                                                                                <  SRR4302534.2974007/94‑1 (MQ=60)
               GTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTG                                                                                <  SRR4302534.2596023/94‑1 (MQ=60)
                TTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGC                                                                               >  SRR4302534.2966871/1‑94 (MQ=60)
                 TTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGTCGTGACGTTCACCCCCCTGGGTGGCTGCA                                                                              <  SRR4302534.4298364/94‑1 (MQ=60)
                   TGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAAT                                                                            >  SRR4302534.878905/1‑94 (MQ=60)
                     CCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCA                                                                          >  SRR4302534.4444630/1‑94 (MQ=60)
                     CCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCA                                                                          >  SRR4302534.4742402/1‑94 (MQ=60)
                     CCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCA                                                                          <  SRR4302534.965490/94‑1 (MQ=60)
                      CATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCAT                                                                         >  SRR4302534.3465668/1‑94 (MQ=60)
                       CTCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATC                                                                        <  SRR4302534.1658030/94‑1 (MQ=60)
                        TCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCG                                                                       <  SRR4302534.2818169/94‑1 (MQ=60)
                        TCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCG                                                                       <  SRR4302534.3785076/94‑1 (MQ=60)
                         CGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGC                                                                      >  SRR4302534.6537129/1‑94 (MQ=60)
                         CGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGC                                                                      >  SRR4302534.1054240/1‑94 (MQ=60)
                           CCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTT                                                                    >  SRR4302534.6065573/1‑94 (MQ=60)
                              GAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTgtca                                                                 >  SRR4302534.5841497/1‑90 (MQ=60)
                              GAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCA                                                                 >  SRR4302534.5720872/1‑94 (MQ=60)
                              GAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCA                                                                 >  SRR4302534.2865262/1‑94 (MQ=60)
                               AGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCAC                                                                <  SRR4302534.6328522/94‑1 (MQ=60)
                                GATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACC                                                               <  SRR4302534.3443173/94‑1 (MQ=60)
                                 ATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCG                                                              <  SRR4302534.199175/94‑1 (MQ=60)
                                  TCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGT                                                             >  SRR4302534.5945355/1‑94 (MQ=60)
                                   CCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTT                                                            >  SRR4302534.675655/1‑94 (MQ=60)
                                   CCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTT                                                            <  SRR4302534.3787418/94‑1 (MQ=60)
                                   CCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTT                                                            >  SRR4302534.2514232/1‑94 (MQ=60)
                                   CCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTT                                                            >  SRR4302534.5180821/1‑94 (MQ=60)
                                      CCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCT                                                         <  SRR4302534.4806360/94‑1 (MQ=60)
                                        AACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTC                                                       <  SRR4302534.6176825/94‑1 (MQ=60)
                                        AACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTC                                                       <  SRR4302534.2109022/94‑1 (MQ=60)
                                        AACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTC                                                       <  SRR4302534.2567301/94‑1 (MQ=60)
                                         ACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCG                                                      >  SRR4302534.1248347/1‑94 (MQ=60)
                                         ACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCG                                                      >  SRR4302534.3964845/1‑94 (MQ=60)
                                          CTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGC                                                     <  SRR4302534.6297263/94‑1 (MQ=60)
                                          CTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGC                                                     >  SRR4302534.3485936/1‑94 (MQ=60)
                                           TGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCC                                                    >  SRR4302534.233198/1‑94 (MQ=60)
                                           TGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCC                                                    >  SRR4302534.4466066/1‑94 (MQ=60)
                                             GCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCAC                                                  <  SRR4302534.4366337/94‑1 (MQ=60)
                                                 TCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTC                                              <  SRR4302534.3180007/94‑1 (MQ=60)
                                                  CACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             <  SRR4302534.4102156/94‑1 (MQ=60)
                                                  CACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             <  SRR4302534.4610610/94‑1 (MQ=60)
                                                  CACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             >  SRR4302534.142807/1‑94 (MQ=60)
                                                  CACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCG                                             >  SRR4302534.2468648/1‑94 (MQ=60)
                                                    CTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGC                                           <  SRR4302534.2632034/94‑1 (MQ=60)
                                                     TTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCA                                          <  SRR4302534.361888/94‑1 (MQ=60)
                                                     TTTCGCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATTATCGCTTCCACCGTTCCTTCGCCACCGTCGGCA                                          <  SRR4302534.6492726/94‑1 (MQ=60)
                                                        CTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACC                                       <  SRR4302534.1665668/94‑1 (MQ=60)
                                                         TCGCCCAGCTGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCG                                      <  SRR4302534.2952699/94‑1 (MQ=60)
                                                         TCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCG                                      <  SRR4302534.482575/94‑1 (MQ=60)
                                                           GCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGA                                    >  SRR4302534.5910135/1‑94 (MQ=60)
                                                           GCCCAGCGCCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGA                                    >  SRR4302534.377071/1‑94 (MQ=60)
                                                            CGCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGCGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAA                                   >  SRR4302534.6165123/1‑94 (MQ=60)
                                                            CCCAGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAA                                   >  SRR4302534.2752551/1‑94 (MQ=60)
                                                               acgtGCCCTGTAACCCAGGCGTGACGTTCACCGCCCTGTGTGGCTGGAATCATCGCTTCCACCGTTCCTACGCGACCGTCGGCAGCCGGAACAG                                <  SRR4302534.2491811/90‑1 (MQ=60)
                                                               AGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAG                                <  SRR4302534.2551690/94‑1 (MQ=60)
                                                               AGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAG                                <  SRR4302534.1733606/94‑1 (MQ=60)
                                                               AGCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAG                                >  SRR4302534.6526648/1‑94 (MQ=60)
                                                                GCGGCCCTGTAACCGAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGA                               <  SRR4302534.4481512/94‑1 (MQ=60)
                                                                GCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGA                               <  SRR4302534.328051/94‑1 (MQ=60)
                                                                GCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGA                               <  SRR4302534.4481513/94‑1 (MQ=60)
                                                                GCGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGA                               <  SRR4302534.2911570/94‑1 (MQ=60)
                                                                 CGGCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAA                              <  SRR4302534.1790984/94‑1 (MQ=60)
                                                                   GCCCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAAC                            <  SRR4302534.1337336/94‑1 (MQ=60)
                                                                    CCCTGTAGCCCAGGGGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCGCCGTTCCTTCGCCACCGTCGGCAGCCGGAACAGAAACG                           <  SRR4302534.4854734/94‑1 (MQ=60)
                                                                     CCTGTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGT                          >  SRR4302534.6333780/1‑94 (MQ=60)
                                                                        GTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACT                       <  SRR4302534.3526944/94‑1 (MQ=60)
                                                                        GTAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACT                       <  SRR4302534.6160677/94‑1 (MQ=60)
                                                                         TAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTG                      >  SRR4302534.6270347/1‑94 (MQ=60)
                                                                         TAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTG                      <  SRR4302534.245559/94‑1 (MQ=60)
                                                                         TAACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTG                      >  SRR4302534.5830218/1‑94 (MQ=60)
                                                                          AACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGT                     >  SRR4302534.2822350/1‑94 (MQ=60)
                                                                          AACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGT                     <  SRR4302534.2885715/94‑1 (MQ=60)
                                                                          AACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGT                     >  SRR4302534.1841999/1‑94 (MQ=60)
                                                                           ACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTG                    <  SRR4302534.5087079/94‑1 (MQ=60)
                                                                           ACCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTG                    <  SRR4302534.952419/94‑1 (MQ=60)
                                                                            CCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGC                   <  SRR4302534.2777460/94‑1 (MQ=60)
                                                                            CCCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGC                   <  SRR4302534.571267/94‑1 (MQ=60)
                                                                             CCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAAGAGAAACGAACTGTGCT                  >  SRR4302534.3456155/1‑94 (MQ=60)
                                                                             CCAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCA                  >  SRR4302534.5895478/1‑94 (MQ=60)
                                                                              CAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAT                 >  SRR4302534.5395925/1‑94 (MQ=60)
                                                                              CAGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCAT                 <  SRR4302534.3490769/94‑1 (MQ=60)
                                                                               TGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATC                >  SRR4302534.829453/1‑94 (MQ=60)
                                                                               AGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATC                >  SRR4302534.4334110/1‑94 (MQ=60)
                                                                               AGGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATC                >  SRR4302534.3175520/1‑94 (MQ=60)
                                                                               AGGCGTGACGTTCACCCACCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATC                <  SRR4302534.4594358/94‑1 (MQ=60)
                                                                                GGCGTGACGTTCACCCCCCTGGGTGTCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCA               <  SRR4302534.1411163/94‑1 (MQ=60)
                                                                                GGCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCA               <  SRR4302534.3864675/94‑1 (MQ=60)
                                                                                 GCGTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAG              <  SRR4302534.3644414/94‑1 (MQ=60)
                                                                                   GTGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGA            >  SRR4302534.2723654/1‑94 (MQ=60)
                                                                                    TGACGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAA           <  SRR4302534.336867/94‑1 (MQ=60)
                                                                                       CGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAA        >  SRR4302534.4689349/1‑94 (MQ=60)
                                                                                       CGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAA        >  SRR4302534.5072559/1‑94 (MQ=60)
                                                                                       CGTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAA        >  SRR4302534.3092396/1‑94 (MQ=60)
                                                                                        GTTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAAT       >  SRR4302534.4602383/1‑94 (MQ=60)
                                                                                         TTCACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATT      >  SRR4302534.313061/1‑94 (MQ=60)
                                                                                            ACCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCC   >  SRR4302534.2294673/1‑94 (MQ=60)
                                                                                             CCCCCCTGGTTGCCTCCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGCAACAGAAACGTCCTGTGCATCAGGAAAAATTTCCC  <  SRR4302534.4696347/94‑1 (MQ=60)
                                                                                             CCCCCCTGGGTGGCTGCAATCCTCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  <  SRR4302534.1203674/94‑1 (MQ=60)
                                                                                             CCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  SRR4302534.6089430/1‑94 (MQ=60)
                                                                                             CCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  SRR4302534.871836/1‑94 (MQ=60)
                                                                                             CCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  SRR4302534.2982130/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCC  >  NC_000913/3269143‑3269329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑